ライフサイエンスコーパス: pulldown assay

1 Plk1 decreased its affinity for IKKgamma in pulldown assay.

2 or PP2A-B) using a glutathione S-transferase-pulldown assay.

3 active state of AIP1 assessed by an in vitro pulldown assay.

4 ation in the glutathione S-transferase (GST) pulldown assay.

5 ction by the glutathione S-transferase (GST) pulldown assay.

6 endogenous ARF6, but not ARF1, using a novel pulldown assay.

7 n vitro by a glutathione S-transferase (GST) pulldown assay.

8 d to form a stable complex with SpoIVFB in a pulldown assay.

9 led-coil domain of MuRF1 was demonstrated by pulldown assay.

10 f apoE, as determined by an in vitro heparin pulldown assay.

11 ently confirmed by glutathione S-transferase pulldown assays.

12 nal domain of RPW8.2, which was confirmed by pulldown assays.

13 oprecipitation and glutathione S-transferase pulldown assays.

14 tion with Akt2 by co-immunoprecipitation and pulldown assays.

15 al 44 amino acids of PDZD11, as shown by GST-pulldown assays.

16 iation of PG with ANX II was demonstrated in pulldown assays.

17 and SHP-2 phosphatase activity, and in vitro pulldown assays.

18 determined by yeast two-hybrid assays and by pulldown assays.

19 n, a result that was confirmed by GST-fusion pulldown assays.

20 ast two-hybrid and glutathione S-transferase pulldown assays.

21 h phosphatase abolishes their association in pulldown assays.

22 and in vitro glutathione S-transferase (GST) pulldown assays.

23 otein complex immunoprecipitation and biotin pulldown assays.

24 ), as shown by yeast two-hybrid and in vitro pulldown assays.

25 by using RNA immunoprecipitation and biotin pulldown assays.

26 cence, flow cytometry, real-time RT-PCR, and pulldown assays.

27 co-immunoprecipitation and in vitro protein pulldown assays.

28 d as the 14-3-3 binding region by GST-14-3-3 pulldown assays.

29 d the TM 4,5-loop was demonstrated using GST pulldown assays.

30 1 for binding to both PP2Acalpha isoforms in pulldown assays.

31 th E2 and interacted only weakly with NS3 in pulldown assays.

32 n protein (amino acids 475-589) on liposomal pulldown assays.

33 s by confocal microscopy and in an in vitro "pulldown" assay.

34 In vitro pulldown assays also indicate that DinB(C66A) binds RecA

35 The pulldown assays also indicated the presence of Cox16p in

36 Interaction was confirmed by a GST pulldown assay and by coimmunoprecipitation in human H29

37 s was confirmed by glutathione S-transferase pulldown assay and co-immunoprecipitation assay in human

38 rabidopsis and Nicotiana benthamiana using a pulldown assay and fluorescence resonance energy transfe

39 em and an in vitro glutathione-S-transferase pulldown assay and observed interactions between cyclin

40 egion of AKAP79 was able to bind PP1 by both pulldown assay and surface plasmon resonance.

41 interaction by site-directed mutagenesis and pulldown assay and thereby confirm that the major bindin

42 Furthermore, our results from both GST pulldown assays and analytical ultracentrifugation show

43 and NRL-leucine zipper was validated by GST pulldown assays and co-immunoprecipitation from bovine r

44 ee classes of aptamers bound to Smads by GST pulldown assays and co-immunoprecipitation from mammalia

45 using a combination of affinity interaction pulldown assays and co-immunoprecipitations from brain a

46 In vitro pulldown assays and coimmunoprecipitation analysis furth

47 een aldolase and SUR was confirmed using GST pulldown assays and coimmunoprecipitation assays.

48 Using glutathione S-transferase pulldown assays and coimmunoprecipitation techniques, we

49 nteracts physically with Abl and Trio in GST-pulldown assays and in co-immunoprecipitation experiment

50 y impaired both the UL54-UL44 interaction in pulldown assays and long-chain DNA synthesis without aff

51 Using pulldown assays and mass spectrometry, we have identifie

52 e in eIF5 interaction with eIF1 and eIF3c in pulldown assays and reduces the eIF5-mediated stimulatio

53 70-binding site in SOD2, we used a series of pulldown assays and showed that hsp70 binds to the amino

54 in two-hybrid and glutathione S-transferase pulldown assays and that interaction with the mutant TFI

55 DM2 required for p21(Waf1) degradation using pulldown assays and Western blotting and then examined t

56 ch as affinity precipitation of protein, GST-pulldown assay, and coimmunoprecipitation of proteins, w

57 ctivity enzyme-linked immunosorbent assay, a pulldown assay, and immunostaining with a monoclonal ant

58 Interactions were verified in pulldown assays, and colocalization with PC-TP was confi

59 ith MLF1 by yeast two-hybrid analysis and in pulldown assays, and colocalizes with it in both the nuc

60 nity chromatography, co-immunoprecipitation, pulldown assays, and enzyme-linked immunosorbent assay,

61 Here, using size-exclusion chromatography, pulldown assays, and small angle x-ray scattering, we sh

62 brid mating and co-transformation protocols, pulldown assays, and surface plasmon resonance analysis.

63 ient transfection, yeast two-hybrid, and GST pulldown assays are used to show not only that nuclear r

64 s work establishes the single-molecule lipid pulldown assay as a simple and highly sensitive approach

65 Complementary to this, we applied pulldown assays as well as microscale thermophoresis as

66 ation analysis, immunoprecipitation, and GST pulldown assays based on the theoretical predictions rev

67 Similarly, a glutathione S-transferase pulldown assay between DNMT1 and Sp1 demonstrates a dire

68 Size exclusion chromatography and pulldown assays both indicate that the lower pH conforma

69 d system, and bound Ckidelta and -epsilon in pulldown assays but did not interact with Ckialpha.

70 n was confirmed by glutathione-S-transferase pulldown assays, by coimmunoprecipitation, and by actin

71 We used L-selectin cytoplasmic tail peptide pulldown assays combined with high sensitivity liquid ch

72 Using pulldown assays combined with mass spectrometry analysis

73 oprecipitation and glutathione S-transferase pulldown assays confirm the complex formation between TB

74 Glutathione S-transferase pulldown assays confirmed a direct interaction between H

75 Co-immunoprecipitation and pulldown assays confirmed PKC and beta-catenin as bindin

76 Both coimmunoprecipitation and in vitro pulldown assays confirmed that ASAP1 directly binds to F

77 Pulldown assays confirmed that the binding between the p

78 Pulldown assays confirmed the presence of newly translat

79 In vitro pulldown assays confirmed this interaction, which was fo

80 Co-immunoprecipitation and pulldown assays coupled with site-directed mutagenesis d

81 In pulldown assays, CR binding to fusion proteins containin

82 Glutathione transferase pulldown assays demonstrate that RPD3 binds directly to

83 GST pulldown assays demonstrate that the dimerization domain

84 Glutathione S-transferase pulldown assays demonstrated direct Nov-BMP interactions

85 Pulldown assays demonstrated interaction between betaCaM

86 erminal kinase domain combined with in vitro pulldown assays demonstrated that eriodictyol, a flavano

87 Pak-CRIB pulldown assays demonstrated that Norbin promotes the P-

88 and glutathione S-transferase fusion protein pulldown assays demonstrated that Rab8 interacted with t

89 RNA pulldown assays demonstrated that SRSF3 binds to an alte

90 Glutathione S-transferase (GST) pulldown assays demonstrated that the hnRNP H NLS intera

91 oprecipitation and glutathione S-transferase pulldown assays demonstrated that the N terminus of ClC-

92 Two-hybrid and pulldown assays demonstrated that UL20, but no other HSV

93 GST pulldown assays demonstrated that vIRF1 interacts with U

94 biophysical methods, including heterocomplex pulldown assays, far-UV CD spectroscopy, the thioflavin

95 ombination of kindlin knockdown, biochemical pulldown assays, fluorescence microscopy, fluorescence r

96 roteins within platelets and confirmation by pulldown assays followed by immunoblotting, we identifie

97 eract with UL44 by glutathione S-transferase pulldown assays, for basal DNA polymerase activity, and

98 Pulldown assays from Arabidopsis thaliana tissue culture

99 GRFdeltaC binds H-Ras.GTP in both pulldown assays from bacterial lysates and by coimmunopr

100 noprecipitation or glutathione S-transferase pulldown assays from detergent-solubilized membrane extr

101 oupled receptor in glutathione S-transferase pulldown assays from rat brain lysates coupled with high

102 oplasmic capping complex was demonstrated by pulldown assays, gel filtration and proximity-dependent

103 Pulldown assays, gel filtration, isothermal titration ca

104 trometry and glutathione S-transferase (GST) pulldown assays identified integrin alpha5 as a novel Sc

105 t with pU(L)6 in a glutathione S-transferase pulldown assay in the absence of other viral proteins an

106 oprecipitation and glutathione S-transferase pulldown assay in vitro.

107 GST pulldown assays in yeast lysates demonstrated physical i

108 A RAS-binding domain pulldown assay indicated that RIT1 A57G and Y89H were hi

109 , isothermal titration calorimetry data, and pulldown assays indicated that CaM-N and CaM-C both can

110 Structural results and pulldown assays indicated that L3 renders an in-built ge

111 In this study, glutathione S-transferase pulldown assays indicated that residues 1 to 68 of UL84

112 findings indicate that the streptavidin-bead pulldown assay is valuable for determining the binding o

113 Pulldown assay of GST-KOPR-C-tail with HA-GEC1 or HA-GAB

114 Pulldown assays of a Orai1-CMBD(W76E) mutant, gel filtra

115 Pulldown assays of epitope-tagged S100A2 and yeast two-h

116 Immunoprecipitation and pulldown assays of purified proteins demonstrated a dire

117 With the use of pulldown assays, PC and ezrin were found to interact dir

118 As shown with affinity pulldown assays, PrgJ and the K471E mutant protein inter

119 Finally, pulldown assays reveal a bipartite physical interaction

120 Co-immunopurification and pulldown assays reveal that P2X4 receptors complex with

121 Coimmunoprecipitation and in vitro pulldown assays reveal that phosphorylation of MyoGEF at

122 Glutathione S-transferase pulldown assays revealed binding of CFTR to alpha-AP-2 (

123 Rho pulldown assays revealed that Cryptococcus induces activ

124 Pulldown assays revealed that either of CSS2A, CSS2B, an

125 pitation and glutathione S-transferase (GST) pulldown assays revealed that GBP1 interacted with the N

126 GST pulldown assays revealed that the central loop of the Na

127 Yeast two-hybrid and direct pulldown assays revealed that the N-terminal domain of t

128 Coimmunoprecipitation and pulldown assays revealed that vimentin interacted with A

129 hione S-transferase-Ras-binding domain (RBD) pulldown assays revealed that, although high-grade TCR s

130 In immunoprecipitation and pulldown assays, ShcA, via its SH2 domain, was associate

131 Results from pulldown assays show that ARF6 exchanges GDP for GTP in

132 Circular dichroism and pulldown assays show that full-length Tat binds to the K

133 Both in vitro and in vivo pulldown assays show that MyoGEF interacts with CSPP.

134 GST pulldown assay showed a direct, ouabain-regulated, and m

135 A pulldown assay showed that S100A4 binds to RAGE in chond

136 Glutathione S-transferase pulldown assay showed that the NH2-terminal ATRX homolog

137 r hemin exporter, results with hemin-agarose pulldown assays showed that Abc3 binds to hemin.

138 Oligo pulldown assays showed that binding of Myc to the Inr el

139 Pulldown assays showed that each C/EBPepsilon isoform in

140 Pulldown assays showed that NS2 forms complexes with bot

141 by absorbance spectroscopy and hemin-agarose pulldown assays showed that Shu1 interacts with hemin, w

142 In vitro and in vivo pulldown assays showed that the carboxyl-terminal region

143 RNA pulldown assays showed that UL84 interacted with IRS1 mR

144 GST-VCP/p97 bound purified PP2A in pulldown assays, showing direct protein-protein interact

145 In vitro translation and pulldown assays suggest direct interaction between BCL10

146 Although pulldown assays suggest that the presence of N- and C-te

147 rectly interacts with importin beta in a GST-pulldown assay, suggesting that the SMN complex might re

148 acted with the helicase domain of BKV Tag in pulldown assays, suggesting that NFI helps recruit Tag t

149 p300 also interacted with Tal1 in protein pulldown assays, suggesting this was a direct interactio

150 sary for PIP3 regulation, and a biochemical "pulldown" assay suggests that PIP3 directly binds this r

151 to embryonic organ explants, with a microRNA pulldown assay that allows direct identification of micr

152 Here, we show by yeast two-hybrid and pulldown assays that SpoVID also interacts directly with

153 selected biochemical pathways; (c) affinity pulldown assays that, in some cases, confirm and even ex

154 ipt in human T cells and found, using biotin pulldown assay, that HuR specifically interacts with its

155 In a pulldown assay, the His-tagged Myb1 interacted with a GS

156 By GST pulldown assays, the interaction domains between HMG2L1

157 In pulldown assays, the rank order of AnkG binding strength

158 Next, we used a rhotekin pulldown assay to confirm directly that IL-1beta deactiv

159 To address this, we used an in vitro pulldown assay to define a series of five arginine resid

160 Rosetta pLysS cells, purified, and used in a pulldown assay to identify interacting proteins from hum

161 We exploited the p21-activated kinase pulldown assay to identify proteins associated with acti

162 al mRNA-binding proteins identified from RNA pulldown assays to determine which of these exhibit bona

163 Here, we used biolayer interferometry and pulldown assays to identify regions of RAG1 necessary fo

164 e interaction with PP2A as demonstrated by a pulldown assay using a fusion of this domain with glutat

165 A pulldown assay using biotin-labeled S100A4 was used to d

166 A luciferase reporter assay and a pulldown assay using biotinylated INS-class I VNTR probe

167 A pulldown assay using purified proteins demonstrates that

168 ly a GST-NF-kappaB p65 fusion protein in GST pulldown assays was tested.

169 Using G protein activity and in vitro pulldown assays we demonstrate that G alpha(i3) is a bet

170 By using a pulldown assay, we observed that STAT6 in WT Th1 cells b

171 g luciferase p-miR-Report constructs and RNA pulldown assays, we confirmed that miR-511 bound directl

172 Using pulldown assays, we demonstrate that SIRT1-Delta2/9 bind

173 Using pulldown assays, we demonstrate that the interaction of

174 Using EMSA, supershift assays, and promoter pulldown assays, we demonstrated that CREB, ATF-2, and c

175 By pulldown assays, we discovered that in addition to the p

176 Furthermore, using pulldown assays, we discovered that Sam68 is a possible

177 By using yeast two-hybrid and in vitro pulldown assays, we have documented that PITX2a can form

178 ce energy transfer experiments, and in vitro pulldown assays, we have now identified the key residues

179 Using the human Cad11 cytoplasmic domain in pulldown assays, we identified human angiomotin (Amot),

180 pitation (co-IP), mass spectrometry, and GST pulldown assays, we identified poly(ADP-ribose) polymera

181 assays, and glutathione S-transferase (GST) pulldown assays, we show that NR2A subunits interact dir

182 depending on its CTD phosphorylation state, pulldown assays were performed using the CTD of the duck

183 tial effect, glutathione S-transferase (GST) pulldown assays were performed, revealing that Y544 is c

184 Glutathione-S-transferase (GST) pulldown assays were used to show that RPTPrho interacts

185 rmed in vitro by a glutathione S-transferase pulldown assay, which allowed us to detect 2C/2C associa

186 V envelope glycoproteins were also used in a pulldown assay with beads coated with heparin, a close H

187 However, our enzyme pulldown assays with different polymeric substrates sugg

188 o-immunoprecipitation, two-hybrid assay, and pulldown assays with expressed proteins.

189 For the active residues, we performed pulldown assays with membrane-impermeable 2-aminoethyl m

190 o-immunoprecipitation with ACCA antibody and pulldown assays with recombinant AKR1B10 protein.

191 ed both in vitro by Far-Western and antibody pulldown assays with recombinant proteins and in vivo by

192 say that combines principles of conventional pulldown assays with single-molecule fluorescence micros

193 We next performed pulldown assays, with GGGGCC5, in conjunction with mass