ライフサイエンスコーパス: pulse label

1 he greatest rate (approximately 90 min after pulse labeling).

2 ction was characterized by hydrogen exchange pulse labeling.

3 ediate was studied by hydrogen exchange (HX) pulse labeling.

4 in the far-UV region and by NMR quench-flow pulse labeling.

5 to levels that were detectable other than by pulse labeling.

6 -UTP and FITC-conjugated CTP within 5 min of pulse labeling.

7 gical volumetry and bromodeoxyuridine (BrdU) pulse labeling.

8 d peripheral T cell proliferation by genetic pulse labeling.

9 early folding events has been analyzed by pH-pulse labeling.

10 al DNA synthesis by bromodeoxyuridine (BrdU) pulse-labeling.

11 of 5-bromo-2-deoxyuridine following in vivo pulse-labeling.

12 ne H3 immunoreactivity and bromodeoxyuridine pulse labelling.

13 mide only under chase labeling but not acute pulse labeling, 3) the induction of the levels of sphing

14 under the conditions used for the [(32)P]GTP pulse labeling, (32)P was incorporated into the entire m

15 Assays for pulse-labeled [3H]DNA and for total DNA indicated that t

16 produce full-length Lhcb as demonstrated by pulse-labeling: a new radioactively labeled band of a si

17 In the absence of substantial SecA, pulse-labeled AcrB was retained in the cytoplasm even af

18 DcxCreER(T2) transgenic mice to permanently pulse-label age-defined cohorts of granule cells born ei

19 Pulse-labeling alkaline elution showed deficiency of dam

20 Pulse-labeling analyses show that formation of prenyl-pA

21 In this study using ex vivo pulse label analysis, we demonstrate that AIPL1 is not i

22 Pulse-labeling analysis disclosed a time-dependent reduc

23 Pulse-labeling analysis indicated that Nodals and Leftys

24 ll kinetic folding, the present work used HX pulse labeling analyzed by a fragment separation-mass sp

25 After a 30-min [35S]methioneine/cysteine pulse labeling and 120 min of chase, all of the nascent

26 Pulse labeling and chase of mitochondrial translation pr

27 he nonprocessed D1 precursor was observed by pulse labeling and immunodetection in LAHG-grown PS I-le

28 H24L/H119F were studied by hydrogen exchange pulse labeling and interrupted hydrogen/deuterium exchan

29 ompared at high resolution by quench-flow pH-pulse labeling and interrupted hydrogen/deuterium exchan

30 In this study, we employed a combined pulse labeling and neutral-neutral two-dimensional gel-b

31 lts from kinetic deuterium hydrogen exchange pulse labeling and protein engineering studies.

32 In organello, pulse labelling and pulse-chase experiments have enabled

33 Pulse labelling and treatment of cells with the translat

34 Pulse-label and pulse-chase experiments show that GPA is

35 that had not been looked at previously were pulse-labeled and analyzed for the presence of newly tra

36 r membrane surface proteins were vectorially pulse-labeled and flagella and vesicles were analyzed fo

37 half-lives than wild-type controls for total pulse-labeled and individual mRNAs.

38 change using 5-bromo-2'-deoxyuridine (BrdU) pulse-labeling and DAPI (4',6-diamidino-2-phenylindole)

39 ons, Western blot (immunoblot) analysis, and pulse-labeling and immunoprecipitation of both fusion pr

40 We report here the results of pulse-labeling and immunoprecipitation studies of this r

41 Pulse-labeling and metabolic chase analysis suggested th

42 is increased by ppGpp as judged by both RpoS pulse-labeling and promoter-independent effects on lacZ

43 during superinduction, yet Western blotting, pulse labeling, and the use of bicistronic vectors showe

44 ransfer activity was inhibited at the end of pulse labeling, apoB100 secretion was abolished.

45 was verified using an in vivo stable isotope pulse-labeling approach, and their exact ribosomal prote

46 uses is polyadenylated like bacterial mRNAs, pulse-labelled as well as the steady-state population of

47 Using a dye (PKH26) to pulse label ATMs in vivo, we purified macrophages recrui

48 Approximately 25% of all pulse-labeled beta-catenin destined for E-cadherin assoc

49 We found that pulse-labeled beta-catenin replaces the beta-catenin bou

50 y inhibit the assembly of apoB-100 VLDL) and pulse-labeled (+BFA) and chased (+BFA) for 30 min to obt

51 ional viral RC, detected by incorporation of pulse-labeled bromodeoxyuridine into newly synthesized D

52 on in HCMV-infected cells, they incorporated pulse-labeled bromodeoxyuridine, and their formation was

53 oteins for anterograde axonal transport were pulse labeled by intravitreous injection of (35)S-methio

54 , which accumulated in the medium, even from pulse labeled cells, was predominantly in the high molec

55 association of MTP and apoB, as assessed in pulse-labeled cells by co-immunoprecipitation, was trans

56 In pulse label-chase experiments, [Cl-] was 19 mM just afte

57 In the present study, we pulse-labeled chick embryos by injecting low doses of th

58 or 14 days after crushing the sciatic nerve; pulse-labeled class II and class III beta-tubulin were i

59 pped-flow fluorescence and hydrogen exchange pulse labeling coupled with mass spectrometry.

60 n T-87 cell lipid assembly were evaluated by pulse labeling cultures with [(14)C]acetate and [(14)C]g

61 ication and endoreduplication in nuclei from pulse-labeled developing maize root tips.

62 iofilms using immunofluorescent detection of pulse-labeled DNA and also an inducible green fluorescen

63 Furthermore, bromodeoxyuridine (BrdU)-pulse-labeled DNA synthesis initiation sites colocalized

64 Nevertheless, replication foci pulse labeled during any short interval of S phase were

65 Previous pulse labeling efforts have always assumed EX2 exchange

66 f the two UDP-sugar substrates separately to pulse label either the beginning or the end of HA chains

67 5 days in labeled medium and also in a 1-day pulse labeling experiment where the washout of label was

68 Here, we revisited the pulse labeling experiment with barnase and detected no s

69 tudies, an amide hydrogen/deuterium exchange pulse-labeling experiment detected a stable submilliseco

70 on of a double-jump experiment followed by a pulse-labeling experiment.

71 e novo sphingolipid pathway as determined by pulse labeling experiments and inhibition studies with m

72 Pulse labeling experiments demonstrated that newly synth

73 nt for NMR measurements after quench-flow pH-pulse labeling experiments gives a greatly increased dat

74 Pulse labeling experiments indicate that int6Delta signi

75 Pulse labeling experiments indicate that LS is synthesiz

76 Hydrogen exchange pulse labeling experiments indicate that, in contrast to

77 Pulse labeling experiments reveal that, in immature cons

78 Pulse labeling experiments revealed that rates of protei

79 tion did not alter PIKK mRNA levels, in vivo pulse labeling experiments showed that Tel2 controls the

80 In pulse labeling experiments, AAI protected TraR against p

81 labeling by amino acids in cell culture and pulse labeling experiments.

82 Finally, pulse labelling experiments demonstrated that metabolic

83 In contrast, pulse labelling experiments of GDF-5-infected limbs show

84 Moreover, yeast pulse-labeling experiments argue against there being a s

85 s observed in the previous hydrogen exchange pulse-labeling experiments at pH 6.2 and 10 degrees C.

86 Consistent with this, pulse-labeling experiments demonstrate a significant red

87 [35S]Methionine pulse-labeling experiments demonstrated that GIRK4 assoc

88 uch that the results of our model agree with pulse-labeling experiments from three different nerve ce

89 Strikingly, pulse-labeling experiments indicate that total poly(A)(+

90 Pulse-labeling experiments indicate that ubiquilin facil

91 BrdU pulse-labeling experiments revealed that virtually all s

92 Hydrogen exchange pulse-labeling experiments show that the slow-folding ph

93 Pulse-labeling experiments showed that expression of at

94 Pulse-labeling experiments showed that in vivo CL biosyn

95 Pulse-labeling experiments showed that most major late p

96 Pulse-labeling experiments showed that newly synthesized

97 5-Iododeoxyuridine/5-chlorodeoxyuridine pulse-labeling experiments showed that RAD6 is necessary

98 In pulse-labeling experiments that followed nascent MYOC ov

99 Pulse-labeling experiments using 5-bromo-2-deoxyuridine

100 Results of pulse-labeling experiments with [35S]methionine further

101 Hydrogen exchange pulse-labeling experiments, with NMR detection, were per

102 lly induced dynamic nuclear polarization NMR pulse-labelling experiments that involve rapid in situ p

103 rogen deuterium exchange (HDX) data from NMR pulsed labelling experiments, and uses backbone and side

104 d a severe reduction in the incorporation of pulse-labelled flagellin into the membrane/flagellum fra

105 Hydrogen exchange pulse-labeling followed by mass spectrometry reveals det

106 hin a cell population can be demonstrated by pulse-labeling followed by PCR amplification of immunopr

107 When HSP16.9 is pulse labeled for 10 ms, residues 1-40 and 131-151 are s

108 First, McA RH7777 cells were pulse-labeled for 20 min with [35S]methionine/cysteine a

109 When pulse-labeled Gag precursors from High Five cells were f

110 alphaherpesvirus gI homologs, a fraction of pulse-labeled gI synthesized in BHV-1-infected cells app

111 d to establish the structure of the PUFs and pulse-labelled HDX NMR was used to show that the PUFs an

112 l phosphates on prothymosin alpha in vivo by pulse-labeling HeLa cells with [32P]orthophosphate and c

113 , and also the recently introduced transient pulse-labeling HX experiments.

114 using circular dichroism, fluorescence, and pulse-labeling hydrogen exchange.

115 Pulse-labeling hydrogen-deuterium exchange experiments m

116 In pulse-labelled hydrogen/deuterium exchange experiments m

117 data set of early folding residues based on pulsed labeling hydrogen deuterium exchange experiments.

118 ived from aldolase incubated in 3 M urea and pulse labeled in (2)H2O.

119 rongylocentrotus droebachiensis embryos were pulse labeled in the presence of colchicine or taxol at

120 Uridine pulse labeling in intact CS-B fibroblasts and lymphoblas

121 Using pulse labeling in vivo and synchronized translation in v

122 nt transport waves obtained by radioisotopic pulse labeling in vivo.

123 fragments derived from folded cytochrome c, pulse-labeled in the same manner, to indicate the percen

124 Pulse-labeling in the presence of cycloheximide indicate

125 no presaturation pulse, (2) a presaturation pulse labeling inferior vena cava (IVC) blood (signal vo

126 Interestingly, shortly after pulse labeling INS cells, a substantial fraction of both

127 ombination of 5-bromo-2'-deoxyuridine (BrDU) pulse labeling, intracellular biocytin labeling, and imm

128 egral membrane proteins, indicating that the pulse-labeled Lhcb is readily integrated into the membra

129 resolution by an advanced hydrogen-exchange pulse-labeling mass-spectrometry method (HX MS).

130 Pmel17 immunoprecipitates from metabolically pulse labeled melanoma cells and melanocytes contain, in

131 ring folding and used this to provide an NMR pulse labeling method for determining structures of fold

132 Key developments over time include the HX pulse labeling method with nuclear magnetic resonance an

133 ic mice overexpressing NF-M by the classical pulse-labeling method using 35S-methionine.

134 ng" method, which is less accurate than the "pulse-labeling" method typically used in mammals.

135 Pull-down assays of pulse-labeled mitochondria enabled us to characterize Co

136 FE) has been studied using hydrogen exchange pulse labelling monitored by 2D 1H NMR, and by stopped f

137 We also found that the rate at which pulse-labeled monophosphorylated MAPK became bisphosphor

138 More than 50% of pulse-labelled mutant DNA was in short chains characteri

139 In vivo pulse-labeled myosin Va advances along axons at slow tra

140 initiation and elongation, as determined by pulse-labeling nucleotide incorporation in replication f

141 -retaining cells (LRCs) were determined by a pulse label of newborn mice with BrdU, followed by a cha

142 rage of approximately 1,100 RS after a 5-min pulse labeling of 3T3 mouse fibroblast cells in early S-

143 In vivo pulse labeling of an mto1 mutant, however, indicate incr

144 Pulse labeling of ATMs with PKH26 assessed the recruitme

145 thesis by TGF-beta and CTGF was confirmed by pulse labeling of cells with [35S]methionine.

146 Bromodeoxyuridine (BrdU) pulse labeling of cortical slices cultured in NMDA antag

147 Pulse labeling of endothelial cells with cholera toxin B

148 Biosynthetic pulse labeling of five human glycoproteins showed that e

149 In this study, we used stable isotope pulse labeling of human pulmonary artery endothelial cel

150 Western blotting experiments and pulse labeling of infected cells with [(35)S]methionine

151 Pulse labeling of mitochondrial protein synthesis produc

152 lable single-nucleus RNA-Seq (sNuc-Seq) with pulse labeling of proliferating cells by 5-ethynyl-2'-de

153 Immediately after UV irradiation and a short pulse labeling of repair patches, intact nuclei were dig

154 n analysis of ATPase transcripts and in vivo pulse labeling of the mitochondrial translation products

155 Pulse labeling of tumor and TDLN T cells with BrdU confi

156 Pulse labelling of CDP-DG and PG, shown previously to in

157 However, pulse labelling of PS, which normally increases during i

158 Subcellular fractionation after pulse labelling of the cells with 125I-ABL for 2 h at 4

159 Pulse-labeling of cell wall glucans indicated wall synth

160 Pulse-labeling of DNA with EdU and RNA with BrU and test

161 Pulse-labeling of infected cells revealed that LEF-12 mu

162 Pulse-labeling of lpt1Delta strains showed a 30% reducti

163 Metabolic pulse-labeling of nascent RNA with 4'Thiouridine was use

164 Pulse-labeling of progenitors with bromodeoxyuridine sho

165 tu hybridization analyses to detect mRNA and pulse-labeling of proteins were used to examine several

166 We also note that the pulse-labelling pattern of proteins at 42 C (displayed o

167 hosphatase, whereas in F- cells, < 5% of the pulse-labelled polypeptide was processed.

168 aline phosphatase activities and patterns of pulse-labelled polypeptides indicated that TraA'-'PhoA-1

169 retory granules, ISGs): Inhibiting export of pulse-labeled POMC by brefeldin A (BFA) or a 20 degrees

170 eta-cell lines, we have followed the fate of pulse-labeled procathepsin B (ProB, a lysosomal proenyzm

171 ysis of the peptic peptides derived from the pulse-labeled product of the sub-millisecond folding rea

172 ed by Western blot or immunoprecipitation of pulse-labeled protein.

173 PC precursors were detected among pulse-labeled proteins in transformants with N-terminal

174 Furthermore, analysis of pulse-labeled proteins indicated prolonged synthesis of

175 n viral protein accumulation were studied by pulse-labeling proteins in infected protoplasts.

176 Using a novel pulse labeling/quantitative mass spectrometry technique,

177 However, short pulse labeling revealed that the initial translation rat

178 Multipoint BrdU pulse-labeling revealed that, compared to cells actively

179 Moreover, although pulse-labeled RNA decays normally in orn mutant cells un

180 At 44 degrees C, the half-life of total pulse-labeled RNA rose from 2.9 min in a wild-type strai

181 Pulse-labeling RNA studies showed a PARP-dependent incre

182 led to a decrease in the half-life of total pulse-labelled RNA along with decreased half-lives of th

183 We found that about 85% of pulse-labeled rp mRNA was associated with polysomes in e

184 Pulse labeling showed that the rate of recruitment of ne

185 zonal centrifugation.; (ii) fractionation of pulse-labeled steady-state cultures according to cell ag

186 along with a 5-bromo-2'-deoxyuridine (BrdU) pulse-label strategy, we compared memory cells to their

187 We pulse-labeled striosomal cells (S cells) and matrix cell

188 Second, pulse labeling studies demonstrated increased production

189 Pulse labeling studies indicated that newly replicated m

190 PtdIns dramatically in both steady-state and pulse labeling studies, suggesting that the observed eff

191 Immunofluorescence and pulse labelling studies indicate that this is a previous

192 Pulse-labeling studies and immunoblot analyses showed th

193 We have used pulse-labeling studies and the expression of the ARG8(m)

194 m unloading, making it a suitable tracer for pulse-labeling studies of phloem transport.

195 Pulse-labeling studies reveal that extracellular secreti

196 Pulse-labeling studies show that when K(IR)6.2 is expres

197 Results from our pulse-labeling studies showed that Cdc37 protects nascen

198 BrdU, although cell cycle analyses and BrdU pulse-labeling studies suggested that most of this proli

199 d stability of the molecule, as indicated by pulse-labeling studies, demonstrating a prolonged half-l

200 For the G8 mutant, from these assays and pulse-labeling studies, we determined the ratio of synth

201 By performing BrdU pulse-labeling studies, we found that MBC formation prec

202 ntracellular betaPPs and Abeta shortly after pulse labeling suggests that Abeta is produced in the se

203 blood (signal void), and (3) a presaturation pulse labeling superior vena cava (SVC) blood.

204 onses, we used radioactive orthophosphate to pulse-label suspension-cultured cells of Arabidopsis in

205 use footpads using a newly developed in situ pulse labeling technique.

206 Here we used steady-state and pulse-labeling techniques to follow Notch receptors in s

207 the IL-6(-/-) mice show more hepatocyte BrdU pulse labeling than the IL-6(+/+) controls at 24 hours,

208 We pulse-labelled the soil surrounding wheat (Triticum aest

209 Within 10 min of pulse labeling, the mutant protein undergoes a molecular

210 tivities with gp160 at different times after pulse-labeling, the MAbs were sorted into groups that ex

211 xamined by sucrose gradient fractionation of pulse-labeled thylakoids; the accumulation of high-molec

212 We use live imaging and pulse labeling to quantitatively determine the fates of

213 Thus, we were able to use FSPM pulse-labeling to localize PBP4 activity in live cells,

214 We used (13) C pulse-labelling to trace assimilated C in mosses (Sphagn

215 About 70% of the pulse-labelled TraA-'PhoA-121 polypeptide was rapidly pr

216 Pulse-labeled transcription complexes elongated in the p

217 Pulse-labeled transcription complexes established from i

218 intermediate promoter but is observed using pulse-labeled transcription complexes initiated from all

219 We used 5-ethynyluridine to pulse-label transcripts during mitosis and mitotic exit

220 ys, and also strongly influenced the fate of pulse-labeled TraR.

221 In contrast, only 50% of pulse-labeled type I receptor is converted to the Golgi-

222 Vascular SMCs were genetically pulse-labeled using the tamoxifen-dependent Cre recombin

223 Hydrogen exchange pulse labeling was used to establish the structure of th

224 baculoviruses (BV) were plaque purified, and pulse-labeling was used to verify the synthesis and secr

225 hetic rate, measured using [(35)S]methionine pulse labeling, was decreased by 75% in the diabetic adi

226 Release of ABL from the cell, after pulse labelling, was assessed using both fluorescein iso

227 Using SNAP-based fluorescent pulse labeling, we now demonstrate that cell cycle-restr

228 Here, using 13CO2 pulse labeling, we show that natural densities of the nu

229 ng intermediates were probed by H/D exchange pulsed labeling, which showed the coexistence of noncomp

230 e from cytoplasm to chloroplast, Euglena was pulse labeled with 35S-sulfate and the organelles were s

231 maize seedlings at the third-leaf stage were pulse labeled with [(14)C]O(2) and Golgi membranes were

232 Asynchronously cycling cells are pulse labeled with the nucleotide analog 5-bromo-2-deoxy

233 Pulse labeling with [(35)S]methionine and biotinylation

234 The 41-kDa species, emerging within 5 min of pulse labeling with [(35)S]methionine, is converted into

235 y incorporated into camalexin during a 1.5-h pulse labeling with [14C]anthranilate also increased wit

236 incorporation into camalexin during a 1.5-h pulse labeling with [14C]indole was similar to that with

237 TP photoaffinity inhibitor BMS-192951 before pulse labeling with [35S]methionine/cysteine led to an 8

238 Following pulse labeling with [3H]arachidonic acid ([3H]AA), its i

239 Single pulse labeling with [3H]thymidine at 36 h labeled Thy 1-

240 Pulse labeling with [3H]thymidine confirmed in vitro neu

241 Single as well as multiple pulse labeling with [3H]thymidine confirmed that the ent

242 ease H was studied by hydrogen exchange (HX) pulse labeling with analysis by an advanced fragment sep

243 n 17 HIV-infected patients by 30 min in vivo pulse labeling with bromodeoxyuridine (BrdU).

244 x HIV-1-infected patients studied by in vivo pulse labeling with bromodeoxyuridine.

245 Recent work using HX pulse labeling with MS analysis finds that a number of p

246 ucted by genome-wide molecular combing after pulse labeling with two thymidine analogues.

247 s transduced with HPV-18 E7 were pulse-chase-pulse-labeled with (3)H-thymidine ((3)H-TdR) and bromode

248 Moreover, when intact neurons were pulse-labeled with 3H-labeled sugars at low temperature

249 whereby unsynchronized cell populations are pulse-labeled with 5-bromo-2'-deoxyuridine (BrdU), fract

250 newly synthesized (pro)insulin, islets were pulse-labeled with [(3)H]tyrosine (40 microCi) for 20 mi

251 eated with 62.5 microM 6AN for 21 h and then pulse-labeled with [(35)S]methionine for 1 h, increased

252 riton X-100-fractionated extracts from cells pulse-labeled with [(35)S]methionine indicated that HMW1

253 idine residues at its carboxyl terminus were pulse-labeled with [35S]cysteine, and the labeled norrin

254 Human monocyte-derived macrophages were pulse-labeled with [35S]Met and prepared for affinity ch

255 thesis, WI-38 human diploid fibroblasts were pulse-labeled with [35S]methionine, and calpain was immu

256 Frog retinas were pulse-labeled with [35S]methionine/cysteine and [3H]DHA

257 4 and Chinese hamster ovary (CHO) cells were pulse-labeled with BAC-TMR-dextran by fluid-phase endocy

258 Samples withdrawn during dialysis were pulse-labeled with deuterium to identify unfolded region

259 wing dilution from 8 M urea, the protein was pulse-labeled with deuterium, quenched with acid and mas

260 ation of urea, the amide hydrogen atoms were pulse-labeled with deuterium, the labeled samples were q

261 nd late-replicating chromosomal domains were pulse-labeled with halogenated nucleotides and prelabele

262 periods of time with chlorodeoxyuridine and pulse-labeled with iododeoxyuridine 8 h before tumor rem

263 in a high-speed quenched-flow apparatus and pulse-labeled with protium to identify unfolded regions.

264 Proteins were pulse-labeled with radioactive isotope (35S or 14C) in c

265 Pulse-labeling with (15)N-labeled medium time-stamps the

266 in guanidine hydrochloride (GdHCl) or urea, pulse-labeling with 2H2O and analyzing the intact protei

267 lutions of TIM unfolded in GdHCl or urea and pulse-labeling with 2H2O at different times.

268 ing after 24 hours was preceded by 2 hour of pulse-labeling with 5-bromodeoxyuridine.

269 smission of herpes simplex virus (HSV) using pulse-labeling with ethynyl nucleotides and cycloadditio

270 ra, transducin activation, Western blotting, pulse-labeling with immunoprecipitation, and immunocytoc

271 Pulse-labelled YopE, but not YopQ, could be secreted aft