ライフサイエンスコーパス: pulse labeling

1 he greatest rate (approximately 90 min after pulse labeling).

2 in the far-UV region and by NMR quench-flow pulse labeling.

3 to levels that were detectable other than by pulse labeling.

4 -UTP and FITC-conjugated CTP within 5 min of pulse labeling.

5 gical volumetry and bromodeoxyuridine (BrdU) pulse labeling.

6 d peripheral T cell proliferation by genetic pulse labeling.

7 early folding events has been analyzed by pH-pulse labeling.

8 ction was characterized by hydrogen exchange pulse labeling.

9 ediate was studied by hydrogen exchange (HX) pulse labeling.

10 al DNA synthesis by bromodeoxyuridine (BrdU) pulse-labeling.

11 of 5-bromo-2-deoxyuridine following in vivo pulse-labeling.

12 mide only under chase labeling but not acute pulse labeling, 3) the induction of the levels of sphing

13 under the conditions used for the [(32)P]GTP pulse labeling, (32)P was incorporated into the entire m

14 produce full-length Lhcb as demonstrated by pulse-labeling: a new radioactively labeled band of a si

15 Pulse-labeling alkaline elution showed deficiency of dam

16 Pulse-labeling analyses show that formation of prenyl-pA

17 Pulse-labeling analysis disclosed a time-dependent reduc

18 Pulse-labeling analysis indicated that Nodals and Leftys

19 ll kinetic folding, the present work used HX pulse labeling analyzed by a fragment separation-mass sp

20 After a 30-min [35S]methioneine/cysteine pulse labeling and 120 min of chase, all of the nascent

21 Pulse labeling and chase of mitochondrial translation pr

22 he nonprocessed D1 precursor was observed by pulse labeling and immunodetection in LAHG-grown PS I-le

23 H24L/H119F were studied by hydrogen exchange pulse labeling and interrupted hydrogen/deuterium exchan

24 ompared at high resolution by quench-flow pH-pulse labeling and interrupted hydrogen/deuterium exchan

25 In this study, we employed a combined pulse labeling and neutral-neutral two-dimensional gel-b

26 lts from kinetic deuterium hydrogen exchange pulse labeling and protein engineering studies.

27 change using 5-bromo-2'-deoxyuridine (BrdU) pulse-labeling and DAPI (4',6-diamidino-2-phenylindole)

28 ons, Western blot (immunoblot) analysis, and pulse-labeling and immunoprecipitation of both fusion pr

29 We report here the results of pulse-labeling and immunoprecipitation studies of this r

30 Pulse-labeling and metabolic chase analysis suggested th

31 is increased by ppGpp as judged by both RpoS pulse-labeling and promoter-independent effects on lacZ

32 during superinduction, yet Western blotting, pulse labeling, and the use of bicistronic vectors showe

33 ransfer activity was inhibited at the end of pulse labeling, apoB100 secretion was abolished.

34 was verified using an in vivo stable isotope pulse-labeling approach, and their exact ribosomal prote

35 pped-flow fluorescence and hydrogen exchange pulse labeling coupled with mass spectrometry.

36 n T-87 cell lipid assembly were evaluated by pulse labeling cultures with [(14)C]acetate and [(14)C]g

37 Previous pulse labeling efforts have always assumed EX2 exchange

38 5 days in labeled medium and also in a 1-day pulse labeling experiment where the washout of label was

39 Here, we revisited the pulse labeling experiment with barnase and detected no s

40 tudies, an amide hydrogen/deuterium exchange pulse-labeling experiment detected a stable submilliseco

41 on of a double-jump experiment followed by a pulse-labeling experiment.

42 e novo sphingolipid pathway as determined by pulse labeling experiments and inhibition studies with m

43 Pulse labeling experiments demonstrated that newly synth

44 nt for NMR measurements after quench-flow pH-pulse labeling experiments gives a greatly increased dat

45 Pulse labeling experiments indicate that int6Delta signi

46 Pulse labeling experiments indicate that LS is synthesiz

47 Hydrogen exchange pulse labeling experiments indicate that, in contrast to

48 Pulse labeling experiments reveal that, in immature cons

49 Pulse labeling experiments revealed that rates of protei

50 tion did not alter PIKK mRNA levels, in vivo pulse labeling experiments showed that Tel2 controls the

51 In pulse labeling experiments, AAI protected TraR against p

52 labeling by amino acids in cell culture and pulse labeling experiments.

53 Moreover, yeast pulse-labeling experiments argue against there being a s

54 s observed in the previous hydrogen exchange pulse-labeling experiments at pH 6.2 and 10 degrees C.

55 Consistent with this, pulse-labeling experiments demonstrate a significant red

56 [35S]Methionine pulse-labeling experiments demonstrated that GIRK4 assoc

57 uch that the results of our model agree with pulse-labeling experiments from three different nerve ce

58 Strikingly, pulse-labeling experiments indicate that total poly(A)(+

59 Pulse-labeling experiments indicate that ubiquilin facil

60 BrdU pulse-labeling experiments revealed that virtually all s

61 Hydrogen exchange pulse-labeling experiments show that the slow-folding ph

62 Pulse-labeling experiments showed that expression of at

63 Pulse-labeling experiments showed that in vivo CL biosyn

64 Pulse-labeling experiments showed that most major late p

65 Pulse-labeling experiments showed that newly synthesized

66 5-Iododeoxyuridine/5-chlorodeoxyuridine pulse-labeling experiments showed that RAD6 is necessary

67 In pulse-labeling experiments that followed nascent MYOC ov

68 Pulse-labeling experiments using 5-bromo-2-deoxyuridine

69 Results of pulse-labeling experiments with [35S]methionine further

70 Hydrogen exchange pulse-labeling experiments, with NMR detection, were per

71 Hydrogen exchange pulse-labeling followed by mass spectrometry reveals det

72 hin a cell population can be demonstrated by pulse-labeling followed by PCR amplification of immunopr

73 l phosphates on prothymosin alpha in vivo by pulse-labeling HeLa cells with [32P]orthophosphate and c

74 , and also the recently introduced transient pulse-labeling HX experiments.

75 using circular dichroism, fluorescence, and pulse-labeling hydrogen exchange.

76 Pulse-labeling hydrogen-deuterium exchange experiments m

77 data set of early folding residues based on pulsed labeling hydrogen deuterium exchange experiments.

78 Uridine pulse labeling in intact CS-B fibroblasts and lymphoblas

79 Using pulse labeling in vivo and synchronized translation in v

80 nt transport waves obtained by radioisotopic pulse labeling in vivo.

81 Pulse-labeling in the presence of cycloheximide indicate

82 no presaturation pulse, (2) a presaturation pulse labeling inferior vena cava (IVC) blood (signal vo

83 Interestingly, shortly after pulse labeling INS cells, a substantial fraction of both

84 ombination of 5-bromo-2'-deoxyuridine (BrDU) pulse labeling, intracellular biocytin labeling, and imm

85 resolution by an advanced hydrogen-exchange pulse-labeling mass-spectrometry method (HX MS).

86 ring folding and used this to provide an NMR pulse labeling method for determining structures of fold

87 Key developments over time include the HX pulse labeling method with nuclear magnetic resonance an

88 ic mice overexpressing NF-M by the classical pulse-labeling method using 35S-methionine.

89 ng" method, which is less accurate than the "pulse-labeling" method typically used in mammals.

90 initiation and elongation, as determined by pulse-labeling nucleotide incorporation in replication f

91 rage of approximately 1,100 RS after a 5-min pulse labeling of 3T3 mouse fibroblast cells in early S-

92 In vivo pulse labeling of an mto1 mutant, however, indicate incr

93 Pulse labeling of ATMs with PKH26 assessed the recruitme

94 thesis by TGF-beta and CTGF was confirmed by pulse labeling of cells with [35S]methionine.

95 Bromodeoxyuridine (BrdU) pulse labeling of cortical slices cultured in NMDA antag

96 Pulse labeling of endothelial cells with cholera toxin B

97 Biosynthetic pulse labeling of five human glycoproteins showed that e

98 In this study, we used stable isotope pulse labeling of human pulmonary artery endothelial cel

99 Western blotting experiments and pulse labeling of infected cells with [(35)S]methionine

100 Pulse labeling of mitochondrial protein synthesis produc

101 lable single-nucleus RNA-Seq (sNuc-Seq) with pulse labeling of proliferating cells by 5-ethynyl-2'-de

102 Immediately after UV irradiation and a short pulse labeling of repair patches, intact nuclei were dig

103 n analysis of ATPase transcripts and in vivo pulse labeling of the mitochondrial translation products

104 Pulse labeling of tumor and TDLN T cells with BrdU confi

105 Pulse-labeling of cell wall glucans indicated wall synth

106 Pulse-labeling of DNA with EdU and RNA with BrU and test

107 Pulse-labeling of infected cells revealed that LEF-12 mu

108 Pulse-labeling of lpt1Delta strains showed a 30% reducti

109 Metabolic pulse-labeling of nascent RNA with 4'Thiouridine was use

110 Pulse-labeling of progenitors with bromodeoxyuridine sho

111 tu hybridization analyses to detect mRNA and pulse-labeling of proteins were used to examine several

112 n viral protein accumulation were studied by pulse-labeling proteins in infected protoplasts.

113 Using a novel pulse labeling/quantitative mass spectrometry technique,

114 However, short pulse labeling revealed that the initial translation rat

115 Multipoint BrdU pulse-labeling revealed that, compared to cells actively

116 Pulse-labeling RNA studies showed a PARP-dependent incre

117 Pulse labeling showed that the rate of recruitment of ne

118 Second, pulse labeling studies demonstrated increased production

119 Pulse labeling studies indicated that newly replicated m

120 PtdIns dramatically in both steady-state and pulse labeling studies, suggesting that the observed eff

121 Pulse-labeling studies and immunoblot analyses showed th

122 We have used pulse-labeling studies and the expression of the ARG8(m)

123 m unloading, making it a suitable tracer for pulse-labeling studies of phloem transport.

124 Pulse-labeling studies reveal that extracellular secreti

125 Pulse-labeling studies show that when K(IR)6.2 is expres

126 Results from our pulse-labeling studies showed that Cdc37 protects nascen

127 BrdU, although cell cycle analyses and BrdU pulse-labeling studies suggested that most of this proli

128 d stability of the molecule, as indicated by pulse-labeling studies, demonstrating a prolonged half-l

129 For the G8 mutant, from these assays and pulse-labeling studies, we determined the ratio of synth

130 By performing BrdU pulse-labeling studies, we found that MBC formation prec

131 ntracellular betaPPs and Abeta shortly after pulse labeling suggests that Abeta is produced in the se

132 blood (signal void), and (3) a presaturation pulse labeling superior vena cava (SVC) blood.

133 use footpads using a newly developed in situ pulse labeling technique.

134 Here we used steady-state and pulse-labeling techniques to follow Notch receptors in s

135 the IL-6(-/-) mice show more hepatocyte BrdU pulse labeling than the IL-6(+/+) controls at 24 hours,

136 Within 10 min of pulse labeling, the mutant protein undergoes a molecular

137 tivities with gp160 at different times after pulse-labeling, the MAbs were sorted into groups that ex

138 We use live imaging and pulse labeling to quantitatively determine the fates of

139 Thus, we were able to use FSPM pulse-labeling to localize PBP4 activity in live cells,

140 Hydrogen exchange pulse labeling was used to establish the structure of th

141 baculoviruses (BV) were plaque purified, and pulse-labeling was used to verify the synthesis and secr

142 hetic rate, measured using [(35)S]methionine pulse labeling, was decreased by 75% in the diabetic adi

143 Using SNAP-based fluorescent pulse labeling, we now demonstrate that cell cycle-restr

144 Here, using 13CO2 pulse labeling, we show that natural densities of the nu

145 ng intermediates were probed by H/D exchange pulsed labeling, which showed the coexistence of noncomp

146 Pulse labeling with [(35)S]methionine and biotinylation

147 The 41-kDa species, emerging within 5 min of pulse labeling with [(35)S]methionine, is converted into

148 y incorporated into camalexin during a 1.5-h pulse labeling with [14C]anthranilate also increased wit

149 incorporation into camalexin during a 1.5-h pulse labeling with [14C]indole was similar to that with

150 TP photoaffinity inhibitor BMS-192951 before pulse labeling with [35S]methionine/cysteine led to an 8

151 Following pulse labeling with [3H]arachidonic acid ([3H]AA), its i

152 Single pulse labeling with [3H]thymidine at 36 h labeled Thy 1-

153 Pulse labeling with [3H]thymidine confirmed in vitro neu

154 Single as well as multiple pulse labeling with [3H]thymidine confirmed that the ent

155 ease H was studied by hydrogen exchange (HX) pulse labeling with analysis by an advanced fragment sep

156 n 17 HIV-infected patients by 30 min in vivo pulse labeling with bromodeoxyuridine (BrdU).

157 x HIV-1-infected patients studied by in vivo pulse labeling with bromodeoxyuridine.

158 Recent work using HX pulse labeling with MS analysis finds that a number of p

159 ucted by genome-wide molecular combing after pulse labeling with two thymidine analogues.

160 Pulse-labeling with (15)N-labeled medium time-stamps the

161 in guanidine hydrochloride (GdHCl) or urea, pulse-labeling with 2H2O and analyzing the intact protei

162 lutions of TIM unfolded in GdHCl or urea and pulse-labeling with 2H2O at different times.

163 ing after 24 hours was preceded by 2 hour of pulse-labeling with 5-bromodeoxyuridine.

164 smission of herpes simplex virus (HSV) using pulse-labeling with ethynyl nucleotides and cycloadditio

165 ra, transducin activation, Western blotting, pulse-labeling with immunoprecipitation, and immunocytoc