1 s were determined using (35) S radiolabeling
pulse chase.
2 rasynaptic components would be compared with
pulse-chase (
15)N labeling in mice and (14)C content in
3 However,
pulse-chase 5-bromodeoxyuridine-labeling assay revealed
4 Herein, we develop ratiometric
pulse-chase amidination mass spectrometry (rPAm-MS) as a
5 In the latter samples,
pulse-chase analyses demonstrated the disappearance of e
6 e determine translation rates of messages by
pulse-chase analyses in living Escherichia coli cells an
7 Pulse-chase analyses of alpha-Syn half-lives in neurons
8 Pulse-chase analyses of the radiolabeled polypeptides sy
9 Pulse-chase analyses reveal that active site inhibitors
10 Moreover,
pulse-chase analyses reveal that S-palmitoylation is imp
11 Pulse-chase analyses revealed that a portion of Tpk2 kin
12 Pulse-chase analyses revealed that residues 9-20 of the
13 Pulse-chase analyses revealed that the epitope-deleted a
14 Immunoblot and
pulse-chase analyses revealed that the glycosylation-def
15 Pulse-chase analyses revealed that the immature form was
16 In this study,
pulse-chase analyses were used to demonstrate that newly
17 Localization and metabolic
pulse-chase analyses with site-directed mutants and chim
18 Metabolic
pulse-chase analyses, small molecule inhibitor treatment
19 Using a combination of steady state and
pulse-chase analyses, we show that FKBP38 knockdown incr
20 Furthermore, the primer-extension
pulse-chase analysis affirmed that the reconstituted N.
21 However, ex vivo
pulse-chase analysis clearly shows that in the absence o
22 Pulse-chase analysis demonstrated that the absence of th
23 Pulse-chase analysis demonstrates delayed kinetics of 35
24 In agreement, bromodeoxyuridine (BrdU)
pulse-chase analysis demonstrates that the absence of AS
25 Pulse-chase analysis in HEK293F cells indicated that the
26 Pulse-chase analysis indicated that in S6-expressing cel
27 Pulse-chase analysis indicated that the steady-state tur
28 Pulse-chase analysis indicates that these Hsp70/Hsp110 t
29 Fluorescence
pulse-chase analysis of an internally tagged Cse4 reveal
30 HLA-B27 assembly was assessed by
pulse-chase analysis of B27 molecules, and UPR triggerin
31 erived macrophages (MDM) and microglia using
pulse-chase analysis of fibrillar and oligomer (125)I-Ab
32 with orthogonal clickable fluorophores, dual
pulse-chase analysis of Lck revealed accelerated palmita
33 Pulse-chase analysis of p21 protein stability revealed t
34 Pulse-chase analysis of PrP and APP by fluorescent gel i
35 Pulse-chase analysis revealed that all six nsp2 species
36 Pulse-chase analysis revealed that N470D had a prolonged
37 Pulse-chase analysis revealed that PP4 decreased the hal
38 Pulse-chase analysis revealed that pre-termination ribos
39 Pulse-chase analysis revealed that the half-life of CYP1
40 Pulse-chase analysis revealed that the initial particle
41 In addition,
pulse-chase analysis revealed that the proteasome-depend
42 ne was also predominantly extrasynaptic, and
pulse-chase analysis revealed that these newly inserted
43 ion augmented surface CTLA-4 expression, and
pulse-chase analysis showed a more rapid transport of CT
44 Pulse-chase analysis showed that PP4 increased the half-
45 Pulse-chase analysis showed that the rates and amount of
46 Pulse-chase analysis showed that Triton-insoluble, newly
47 In parallel, we used
pulse-chase analysis to measure the transport of two sec
48 temperature to slow the assembly process and
pulse-chase analysis with immunodetection methods, we fo
49 Quantitative imaging,
pulse-chase analysis, and high-resolution ratiometric li
50 yzed by Western blotting, Northern blotting,
pulse-chase analysis, and immunofluorescence to assess t
51 Parabiosis, bromodoxyuridine (BrdU)
pulse-chase analysis, and intranasal instillation of tra
52 As shown through a
pulse-chase analysis, the mdVp1s were derived from the n
53 By
pulse-chase analysis, the prp43 mutant is defective in t
54 Using
pulse-chase analysis, we characterized SMN protein turno
55 Using microscopy-based
pulse-chase analysis, we find that Smo moves through a l
56 Moreover, using
pulse-chase analysis, we show that this activation is ma
57 Pulse chase and arrest of autophagy at the pre-proteolys
58 Pulse chase and mutational analysis indicated that HRD1
59 eased the transcription of Bach1 as shown by
pulse chase and real time PCR experiments.
60 As measured by
pulse-chase and cycloheximide chase assays, a major bind
61 Pulse-chase and cycloheximide experiments revealed that
62 Pulse-chase and cycloheximide-chase assays demonstrated
63 Pulse-chase and endoglycosidase H analysis demonstrate t
64 Pulse-chase and kinetic spectral experiments and modelin
65 Employing histone and nucleotide double-
pulse-chase and lineage tracing, we show that the early
66 We have developed
pulse-chase and mating assays to follow the fate of exis
67 ng and sequencing (Bru-Seq) and bromouridine
pulse-chase and sequencing (BruChase-Seq) to assess geno
68 king to the cell surface tested in metabolic
pulse-chase and surface biotinylation assays, respective
69 rotein processing and stability by metabolic
pulse-chase and surface On-Cell Western blots revealed t
70 We used EdU
pulse-chase and tissue-reconstruction approaches to anal
71 We used stable-isotope
pulse-chase and total proteome mass-spectrometry to iden
72 Pulse-chase application of azidohomoalanine and homoprop
73 Here we show, by combining two different
pulse-chase approaches--genetic fate-mapping with stable
74 A
pulse-chase assay of common SDHB missense mutations in t
75 Moreover, a
pulse-chase assay showed that two chimeric precursors wi
76 We report the development of a
pulse-chase assay to monitor function recovery after che
77 ntaining radioactive auxin to be removed for
pulse chase assays that determine transport rates.
78 on-N-glycosylated F27C variants in metabolic
pulse-chase assays coupled with flow cytometry and cell
79 (3)H-uridine
pulse-chase assays demonstrate that BLM expression is re
80 rrays of immobilized 11-residue peptides and
pulse-chase assays to examine the substrate specificity
81 Pulse-chase assays with BrdU confirmed intracellular rep
82 2)H formation and a lack of equilibration in
pulse-chase assays) were also seen with (rat) P450 2B1,
83 Data from Western immunoblot assays,
pulse-chase assays, and immunoprecipitation assays show
84 Using [(35)S]methionine
pulse-chase assays, we observed that the Isc pathway, bu
85 Pulse-chase biosynthesis experiments demonstrate that re
86 cocaine analog JHC1-64 and by reversible and
pulse-chase biotinylation assays showing evidence for ly
87 Indeed,
pulse-chase biotinylation experiments in IECs lacking An
88 with (35)S-methionine and (35)S-cysteine and
pulse-chase biotinylation of cell surface MRP1 suggests
89 Prox-1 immunohistochemistry and
pulse-chase bromodeoxyuridine labeling showed that proge
90 Pulse-chases combined with mutagenesis studies reveal th
91 neuroendocrine cell line lacking PC1, under
pulse-chase conditions release is constitutive and compo
92 Pulse-chase dye labeling experiments revealed that the e
93 owever, in vitro binding studies utilizing a
pulse-chase electrophoretic mobility shift assay protoco
94 tail loss, we performed a bromodeoxyuridine
pulse-chase experiment and found that a subset of ependy
95 Examination of selected mutants during a
pulse-chase experiment demonstrated an increase in F pro
96 ks the starting point for a crystallographic
pulse-chase experiment of the active site during turnove
97 A
pulse-chase experiment on living cells showed that the r
98 Interestingly, both Western blotting and a
pulse-chase experiment showed co-immunoprecipitation of
99 We employed a carbon-13
pulse-chase experiment to investigate how a temperate es
100 A
pulse-chase experiment was used to demonstrate that even
101 Here, using a (13)C
pulse-chase experiment, we demonstrate how trophic struc
102 cells when compared with control cells in a
pulse-chase experiment.
103 Pulse chase experiments established that the reaction in
104 Pulse chase experiments in 293 T cells expressing rhGAA
105 Pulse chase experiments indicate that the newly-generate
106 Pulse chase experiments show that Tap/NXT significantly
107 Pulse-chase experiments after transferrin uptake showed
108 Using nucleoside
pulse-chase experiments and clonal analysis, we determin
109 EdU
pulse-chase experiments and in vivo tracking of individu
110 Here we performed
pulse-chase experiments and showed that the C29/C30 ster
111 The study shows that (13)CO2
pulse-chase experiments are powerful in elucidating, und
112 Lipid turnover rates were studied by
pulse-chase experiments at the single cell level.
113 Isotope
pulse-chase experiments confirm that all intermediates o
114 Pulse-chase experiments confirm that LRAD3 expression si
115 (14)CO2
pulse-chase experiments confirmed that water deficit enh
116 Pulse-chase experiments confirmed the stress-dependent M
117 Pulse-chase experiments could not detect newly assembled
118 rrest of tumour cell proliferation with TMZ,
pulse-chase experiments demonstrate a tumour re-growth c
119 Last,
pulse-chase experiments demonstrate that Hsp70 preferent
120 Pulse-chase experiments demonstrate that in cells expres
121 EdU
pulse-chase experiments demonstrated a perivascular canc
122 Western immunoblots and
pulse-chase experiments demonstrated that EsaR is stable
123 Pulse-chase experiments demonstrated that in the presenc
124 BrdU
pulse-chase experiments demonstrated that LRCs were dist
125 Pulse-chase experiments demonstrated that NGF treatment
126 Pulse-chase experiments demonstrated that SpoIVFA synthe
127 Pulse-chase experiments demonstrated that the down-regul
128 BrdU
pulse-chase experiments demonstrated the longevity of th
129 biosynthesis of both proteins were similar,
pulse-chase experiments established that the apparent ha
130 In organello, pulse labelling and
pulse-chase experiments have enabled us to track the mit
131 Pulse-chase experiments identified sites of cell surface
132 Pulse-chase experiments in COS-7 cells show that there i
133 Pulse-chase experiments in gonococci demonstrated that L
134 f the elemental effect using dNTPalphaS, and
pulse-chase experiments indicate that a rapid phosphodie
135 Pulse-chase experiments indicated decreased protein turn
136 y of PDI is sufficient for wild-type growth,
pulse-chase experiments monitoring the maturation of car
137 They enable regional optical marking in
pulse-chase experiments on live cells and tissues, and t
138 Pulse-chase experiments reveal a delay in rRNA processin
139 First, BrdU
pulse-chase experiments reveal that CD44(+) cells coloca
140 Immunofluorescence microscopy and
pulse-chase experiments reveal that stabilization of ORF
141 nding occurred at 2.1 muM(-1) s(-1), and the
pulse-chase experiments revealed an ATP-promoted isomeri
142 Pulse-chase experiments revealed that A774wt and avirule
143 Pulse-chase experiments revealed that CPAF was initially
144 Pulse-chase experiments revealed that IE2-86 but not IE1
145 Pulse-chase experiments revealed that newly made protein
146 nously expressing PC1, both steady-state and
pulse-chase experiments revealed that peptides derived f
147 Fate mapping using BrdU
pulse-chase experiments revealed that such deficits may
148 Furthermore,
pulse-chase experiments revealed that the binding of QPD
149 A series of
pulse-chase experiments revealed that the origin of aort
150 HO cells stably expressing the hKOR-N25/39Q,
pulse-chase experiments revealed that the turnover rate
151 Pulse-chase experiments revealed that the turnover rate
152 Rather,
pulse-chase experiments show that decreases in steady st
153 Pulse and
pulse-chase experiments show that IL-2 stimulation resul
154 Metabolic
pulse-chase experiments show that TC10 did not affect CF
155 Pulse-chase experiments showed an enhanced degradation a
156 affinity of the aldehydes for P450 2A6, but
pulse-chase experiments showed only limited exchange wit
157 Pulse-chase experiments showed that a fraction of gO rem
158 Pulse-chase experiments showed that collagen type I secr
159 Significantly,
pulse-chase experiments showed that cotransfection of Nr
160 Pulse-chase experiments showed that PreGN C-terminal cle
161 Pulse-chase experiments showed that SR-BII rapidly inter
162 Pulse-chase experiments showed that the contribution of
163 Pulse-chase experiments showed that the interaction betw
164 Long-term
pulse-chase experiments showed that the mature DeltaRI/D
165 Pulse-chase experiments showed that UT-A1 half-life is r
166 ckdown, REGgamma-deficient MEF analysis, and
pulse-chase experiments substantiate that REGgamma promo
167 Here, we report genetic
pulse-chase experiments that define osteoblastic cells a
168 rd commitment to catalysis was determined by
pulse-chase experiments to be 0.70%.
169 To overcome this difficulty, we used Mg(2+)
pulse-chase experiments to differentiate each reaction i
170 echniques to follow formation of NR by using
pulse-chase experiments to examine protein and lipid del
171 In
pulse-chase experiments to examine the effects of Ad-Acs
172 r organ culture and bromodeoxyuridine (BrdU)
pulse-chase experiments to identify and evaluate stem ce
173 Finally,
pulse-chase experiments using [(14)C]serine revealed tha
174 nucleocapsids into virions as determined in
pulse-chase experiments was dependent on the activity of
175 action time courses, substrate trapping, and
pulse-chase experiments were used to assess folate relea
176 Pulse-chase experiments with (14)CO(2) show that transpo
177 inetic lags were observed in acid formation;
pulse-chase experiments with carrier aldehydes showed on
178 Optical
pulse-chase experiments with Dendra2-tagged aSyn version
179 Through
pulse-chase experiments with halogenated thymidine analo
180 Pulse-chase experiments with radiolabeled sugars and ami
181 Bromodeoxyuridine
pulse-chase experiments with short survival times sugges
182 In
pulse-chase experiments, 14-3-3beta increased the synthe
183 In fact, as directly demonstrated by
pulse-chase experiments, EECs in the vascularized, but n
184 In
pulse-chase experiments, either miR-33 overexpression or
185 In
pulse-chase experiments, recycled peptidoglycan was not
186 In
pulse-chase experiments, the primary Vhs translation pro
187 ng, molecular imaging, and [(35)S]methionine
pulse-chase experiments, together with lysosome (chloroq
188 Using
pulse-chase experiments, we also discovered that the pec
189 lizing in vivo imaging and bromodeoxyuridine
pulse-chase experiments, we have analyzed growth and reg
190 es to different parts of the molecule and in
pulse-chase experiments, we showed that the C terminus o
191 ociation of the intermediates as revealed by
pulse-chase experiments.
192 immunohistochemistry, and bromodeoxyuridine
pulse-chase experiments.
193 r Abeta levels and hasten its degradation in
pulse-chase experiments.
194 ar behavior of these mutants was assessed in
pulse-chase experiments.
195 reaction amplitudes between pulse-quench and
pulse-chase experiments.
196 tect replicase intermediates and products in
pulse-chase experiments.
197 7 +/- 17 and 245 +/- 29 min as determined by
pulse-chase experiments.
198 e observed processivity in pregnenolone/DHEA
pulse-chase experiments.
199 cinal plant Pentalinon andrieuxii by (13)CO2
pulse-chase experiments.
200 track the fate of adult cardiomyocytes in a '
pulse-chase'
fashion: after a 4-OH-tamoxifen pulse, gree
201 Fluorescent
pulse-chase fate-tracking documented dynamic nucleo-cyto
202 We use a new in cellula
pulse-chase imaging protocol with photoactivatable GFP t
203 Pulse-chase,
immunofluorescence, and electron microscopy
204 Pulse-chase immunoprecipitation experiments indicated th
205 Pulse-chase,
immunoprecipitation/immunoblotting analyses
206 Finally, a
pulse-chase isotope enrichment experiment was conducted
207 Pulse-chase kinetic transport assays on four of the top-
208 Acid-quench and
pulse-chase kinetics indicated that an isomerization ste
209 0A on wild-type activity and single-turnover
pulse-chase kinetics.
210 eins bind and initiate assembly prior to the
pulse-chase kinetics.
211 traperitoneally delivered every other day to
pulse-chase label in vivo endogenous cardiac replication
212 Pulse-chase labeling analysis demonstrated that PML over
213 Pulse-chase labeling and cell surface biotinylation expe
214 otein turnover in adipocytes using metabolic
pulse-chase labeling and high resolution mass spectromet
215 We addressed this question using metabolic
pulse-chase labeling and quantitative mass spectrometry
216 A new covalent fluorescent
pulse-chase labeling approach using SNAP tagging has now
217 Pulse-chase labeling demonstrated that the LHDeltaT dime
218 In the present study we use
pulse-chase labeling experiments in conjunction with iso
219 Pulse-chase labeling experiments indicate instead that r
220 n, in vivo lineage tracing, and HSC-specific
pulse-chase labeling have provided novel insights on B1a
221 In vivo
pulse-chase labeling identified galactoglycerolipid pool
222 Pulse-chase labeling indicates that growth is heterogene
223 Improved
pulse-chase labeling of endogenous interphase chromosome
224 Pulse-chase labeling of mitochondrial translation produc
225 Pulse-chase labeling of MRP1 with (35)S-methionine and (
226 Using in vivo
pulse-chase labeling of neutrophil DNA with 6,6-(2)H2-gl
227 In addition,
pulse-chase labeling of Notch in living tissues indicate
228 Here, we describe a metabolic
pulse-chase labeling protocol using 4-thiouracil combine
229 Finally,
pulse-chase labeling reveals that ataxin-3 is degraded b
230 Pulse-chase labeling showed that TRP32 is a stable prote
231 Pulse-chase labeling studies show that in fatb-ko leaves
232 nt AMPA receptor subunits by using two-color
pulse-chase labeling.
233 Pulse-chase labelling experiments with calcein AM sugges
234 epithelial Caco-2 cells using techniques of
pulse-chase labelling, domain-specific biotinylation and
235 he SNAP-tag for studying protein turnover by
pulse-chase labelling.
236 Quantitative
pulse-chase live imaging experiments showed that overexp
237 Immunoblot analysis and
pulse-chase metabolic labeling revealed that hephaestin
238 Pulse-chase metabolic labeling studies demonstrated that
239 scopy, cell-surface cross-linking, FRET, and
pulse-chase metabolic labeling, we demonstrate that dele
240 conflict greatly, with a study employing C14
pulse-chase methodology concluding 1% annual turnover in
241 with amino acids in cell culture (SILAC) and
pulse-chase methods to generate a global quantitative ma
242 We used fluorescence-based
pulse-chase methods to visualize the fate of pre-existin
243 in cells have been carried out with pulse or
pulse-chase methods using radioactive isotopes.
244 Using a novel system for
pulse-chase microscopy, we have visualized the postsynth
245 ng assumptions and restrictive models in the
pulse-chase model artificially eliminated high-turnover
246 Similarly, the
pulse-chase model was acutely sensitive to assumptions o
247 Previously, a
pulse-chase monitored by quantitative mass spectrometry
248 Here we have developed a method involving
pulse-chase monitored by quantitative mass spectrometry
249 in the hippocampal dentate gyrus, using dual
pulse-chase,
multicolour gamma-retroviral tracing, trans
250 cells, we employed an inducible transgenic "
pulse-chase"
murine model (K5Tta x TRE-H2BGFP) to locali
251 Pulse-chase,
Northern blotting, and primer extension ana
252 Using a combination of lineage tracing and
pulse-chase of actively proliferating chondrocytes, we h
253 Pulse-chase photoconversion experiments show that molecu
254 A BrdU
pulse-chasing protocol was also introduced as an additio
255 incorporation into replicating viral DNA and
pulse-chase protocols, we found that viral DNA synthesis
256 The same
pulse/chase protocols were followed by iodixanol subcell
257 r actin, claudin strands break and reanneal;
pulse-chase-
pulse analysis using SNAP-tagged claudins sh
258 Pulse-chase-
pulse experiments with BrdU and EdU, and DNA
259 using a combination of RNA footprinting and
pulse-chase quantitative mass spectrometry paints a pict
260 Pulse-chase radioactive labeling and immunoprecipitation
261 y of IAV-infected cells and biochemically by
pulse-chase radiolabeling experiments.
262 utants and fibroblasts from a human patient,
pulse-chase radiolabeling of newly synthesized proteins
263 Pulse-chase radiolabeling reveals that a ypk1Delta mutan
264 Using
pulse-chase radiolabeling techniques, we find that newly
265 tration, while the half-life was measured by
pulse-chase radiolabelling.
266 also present several lines of evidence (from
pulse-chase,
rapid chemical quench-flow, and stopped-flo
267 kinetic analysis using 14-C tracers and 33-P
pulse-chasing revealed mutation-associated changes in pu
268 findings demonstrate that single time point
pulse-chase SILAC mass spectrometry-based proteomics (pS
269 Using a
pulse-chase SILAC mass spectrometry-based proteomics app
270 oteins in rats were measured in vivo using a
pulse-chase stable isotope labeling experiment.
271 Using a dual
pulse-chase strategy comparing palmitate and protein hal
272 -wide maps of protein synthesis as well as a
pulse-chase strategy for determining rates of translatio
273 pha protein as determined by [35S]methionine
pulse-chase studies by inducing the calcium-activated pr
274 Pulse-chase studies confirmed impaired secretion and inc
275 GH
pulse-chase studies established that the internalized GH
276 Pulse-chase studies found that CYP2E1 protein level is u
277 Pulse-chase studies indicate that PINK1 is rapidly proce
278 Pulse-chase studies indicated that HAX-1 depletion did n
279 Protein turnover was assayed in
pulse-chase studies of 35S-methionine incorporation.
280 Pulse-chase studies of isotopically labeled AChE molecul
281 to 154 decreases pX stability, determined by
pulse-chase studies of WT pX and pX1-140, suggesting tha
282 of rx3 chk mutant/rx3 morphant fish and EdU
pulse-chase studies reveal that rx3 is required to selec
283 Pulse-chase studies revealed that Blebbistatin, a specif
284 Pulse-chase studies showed that osterix-expressing osteo
285 Pulse-chase studies were performed to follow the flow of
286 In contrast,
pulse-chase studies with isotope-labeled nutrients revea
287 Here, we report
pulse-chase studies, which unambiguously establish the o
288 izes pVHL, as shown by protein stability and
pulse-chase studies.
289 A
pulse-chase study of beta(1) integrin biosynthesis indic
290 Pulse-chase study showed that 14-3-3zeta siRNA decreased
291 Using a tetracycline-regulated
pulse-chase system, we measured population turnover rate
292 was investigated in perfused rat liver by a
pulse chase technique.
293 nducible histone2B-green fluorescent protein
pulse-chase techniques, we identify a label-retaining ce
294 mational change, a greater amplitude for the
pulse-chase than the pulse-quench reaction, and an activ
295 By using BrdU
pulse-chase to label S-phase cells and follow their prog
296 opic expression followed by cycloheximide or
pulse-chase treatment demonstrated that phospho-mutants
297 a proteome misfolding stress is applied in a
pulse-chase-
type experiment.
298 Using
pulse-chase vital dye labeling to mark newly forming tee
299 In adult mice administered (15)N-thymidine
pulse-chase,
we find that proliferating crypt cells dilu
300 Pulse-chase with immunoprecipitation analyses revealed a
301 Here, we combine H2B-GFP-based
pulse-chasing with cell-surface markers to distinguish q