ライフサイエンスコーパス: purification

1 well as ligand binding, without the need for purification.

2 the van der Waals interactions upon isotope purification.

3 ed by high-performance liquid chromatography purification.

4 olenes with one equivalent of iodine without purification.

5 rom non-endothelial cells by immune-affinity purification.

6 sters that were directly used in NCL without purification.

7 ically required for catalyst preparation and purification.

8 tion cost and the byproducts released during purification.

9 -molecule precision but without the need for purification.

10 ies were evaluated following chromatographic purification.

11 ligands for inexpensive large-scale protein purification.

12 ng processes less costly and enabling easier purification.

13 r E2-related factor 2 (NRF2) through complex purification.

14 e laborious and time-consuming syntheses and purification.

15 zirconia-coated silica particles for extract purification.

16 at are recalcitrant to classical biochemical purification.

17 nes in good yields, and with straightforward purification.

18 good stability during protein expression and purification.

19 for optimisation of affinity chromatography purification.

20 ilized SpyCatcher-SrtA fusion protein during purification.

21 s for medical diagnostics and pharmaceutical purification.

22 , which require prior in vitro culturing and purification.

23 cations often require suitable selection and purification.

24 complex sample preparation, fragmentation or purification.

25 ied in reconstitution due to difficulties in purification.

26 nt and selective manner without intermediate purification.

27 ire several cleanup steps via chromatography purification.

28 are used to identify cells for analysis and purifications.

29 ter hydrophilic-lipophilic balance cartridge purification, (68)Ga-HZ220 was obtained with a radiochem

30 nd Retro-TRAP (translating ribosome affinity purification), a previously reported molecular profiling

31 dividual cell types typically requires prior purification, a stressful procedure that can itself alte

32 od to access heterocycles without workup and purification after each step.

33 dge assembled with reagents for nucleic acid purification and amplification.

34 ard to the reduced cost of catalyst, polymer purification and by-product recycling.

35 4 and CO2/CH4, with the potential for biogas purification and carbon capture demonstrated for relevan

36 Here, we report purification and characterization of a recombinant PNKP-

37 tical techniques that continue to hinder the purification and characterization of DNA-based structure

38 tally confirm this hypothesis, we report the purification and characterization of the catalytic core

39 omplex is pulled down onto capture beads for purification and concentration.

40 new tools for their recombinant expression, purification and even crystallization.

41 possibilities for the use of GO membranes in purification and filtration technologies.

42 al culture, protein isolation, denaturation, purification and finally protein assembly.

43 Here, we report the purification and functional characterization of the full

44 le one-step protocol was established for the purification and immobilization of SrtA containing a His

45 We employed affinity purification and immunoblotting to validate the interact

46 differentiated, most probably as a result of purification and isolation processes.

47 tein is released, simplifying target protein purification and labeling to a single step.

48 n be applied to both high-efficiency protein purification and large-scale proteomics analysis of cell

49 Accordingly, we used tandem affinity purification and LC-MS/MS to search for novel proteins t

50 lasmid DNA is included during subsequent DNA purification and library preparation steps to prevent lo

51 ipate that Fc-d E1E2 will provide a scalable purification and manufacturing process using protein A/G

52 ssODN-mediated affinity purification and mass spectrometry identified low-abunda

53 Using affinity-purification and mass spectrometry of interactors of the

54 Using immunoaffinity purification and mass spectrometry we show that minispry

55 iprocal tagging in combination with affinity purification and mass spectrometry, we demonstrate that

56 served for a compound that was identified by purification and nuclear magnetic resonance as syringyl

57 In this work, we describe the occurrence, purification and partial characterization of a xylan in

58 Following their purification and physicochemical characterization, the a

59 Purification and quality-control protocols for the oligo

60 be studied in vitro, after their biochemical purification and reconstitution in artificial lipid bila

61 Affinity purification and removal of the Fc tag from E1E2 resulte

62 Using chromatin isolation by RNA purification and sequencing (ChIRP-Seq), we identified M

63 Furthermore, translating ribosome affinity purification and single-cell RNA sequencing identify can

64 A purification and storage protocol was developed to prepa

65 ffect different DNA nanostructures during LC purification and suggest that shear forces may be releva

66 eveloped that significantly simplify product purification and the transfer agent recycling.

67 y reduce energy costs for gas separation and purification and thus facilitate a possible technologica

68 ition, the use of reporter genes allows FACS-purification and tracking of cells that have had multipl

69 Importantly, upon purification and transfer of donor-derived Tregs from an

70 proximity ligation step that eliminates bead purification and washing steps.

71 an be engineered in the absence of intensive purification and/or extensive rounds of design optimizat

72 s in biochemical assays, diagnosis, affinity purification, and drug delivery.

73 We report here the expression, purification, and functional characterization of murine

74 Using cross-linking, affinity purification, and mass spectrometry, we identified the E

75 re need to focus on improved subcultivation, purification, and preservation techniques to recover and

76 neering of phage, liter-scale amplification, purification, and self-templating assembly, and suggest

77 ell preparation, autonomous differentiation, purification, and subsequent differentiation, takes betw

78 thesized oligo pool (479 oligos) without pre-purification, and the error-frequency was reduced from 1

79 hin film composite (TFC) membranes for water purification applications.

80 ve been determined either in bioassay-guided purification approaches or in bioassays with plants in w

81 tional technologies for their extraction and purification are exceedingly energy and chemical intensi

82 large-scale implementation of NMs for water purification, are also highlighted.

83 cellular processes, we performed an affinity purification-based proteomic profiling to identify prote

84 or PCR to generate the library and then bead purification before sequencing.

85 n contents increased with the progression of purification (bulk soil --> crude colloid --> IEF colloi

86 ing flue-gas desulfurization and natural-gas purification, but the design of porous materials with hi

87 phenol-chloroform followed by polyphosphate purification by binding to silica columns or ethanol pre

88 s to the corneal limbus and their subsequent purification by FACS.

89 ting cells for translating ribosome affinity purification by features such as their projections or ac

90 t protein of interest, nanodisc assembly and purification can be achieved within 12-24 h.

91 on of the expressed protein, so that protein purification can be avoided.

92 rcuit-directed translating ribosome affinity purification can be broadly applied to identify molecula

93 and selectivity, and thus provides a record purification capacity for the removal of trace C2 H2 fro

94 ffer from contamination by homodimers posing purification challenges.

95 tation (ChIP) and chromatin isolation by RNA purification (ChIRP) experiments revealed enrichments fo

96 regeneration of toxin-binding albumin by two purification circuits altering the binding capacities of

97 ns in mouse embryonic stem cells by affinity purification coupled to mass spectrometry.

98 ay, we used two "omics" approaches: affinity purification coupled with mass spectrometry and a high t

99 XAP (in vivo cross-linking-assisted affinity purification), coupled with stable isotope labeling with

100 Having access to the purification date can give precious information on the h

101 ater or other unpurified water through water purification, desalination, and distillation.

102 no standardized procedures available for the purification, detection, and characterization of EVs.

103 tural drinking water and an affordable water-purification device is made using the same.

104 inable and cheap option for disposable water purification devices.

105 and includes a kinetic-proofreading step for purification, enabling both enhanced sensitivity and red

106 be immobilized from folding solution without purification, even in the presence of excess staple stra

107 Co-purification experiments of ChlB with Strep-ChlN suggest

108 , which resulted in an 86.3% yield, and 53.8 purification factor.

109 By using translational ribosome affinity purification followed by RNA-Seq, we profiled astroglial

110 sented protocol for histamine extraction and purification followed by SERS analysis coupled with chem

111 asma samples were processed by immunocapture purification, followed by liquid chromatography (LC) wit

112 Moreover, purifications for structural and industrial applications

113 ng a fairly simple and inexpensive method of purification from alkaline acai extract.

114 uire either expression in mammalian cells or purification from immunized mammals.

115 Using affinity purifications from C. albicans cell extracts, we demonst

116 Isotopic purification has enabled quantum coherence times on the

117 ed by specific antibodies for immunoaffinity purification (IAP) and subsequent identification of ubiq

118 se hippocampal neurons by ribosomal affinity purification identified upregulation of chemokine signal

119 mbinase-dependent miRNA tagging and affinity purification in mice.

120 Here, we describe a CRISPR affinity purification in situ of regulatory elements (CAPTURE) ap

121 gy-efficient, adsorbent-based separation and purification in the future.

122 Translating ribosome affinity purification is a method initially developed for profili

123 Protein purification is an essential primary step in numerous bi

124 Leaf protein extraction and purification is applied by other disciplines, such as pr

125 terification and (iv) triacylglycerols (TAG) purification (liquid column chromatography).

126 PAT methods were tested by sampling from two purification locations in a continuous mAb process.

127 Affinity purification mass spectrometry also demonstrated that bo

128 We used affinity purification mass spectrometry to uncover multiple prote

129 oteomics techniques: BioID and Flag affinity purification mass spectrometry.

130 Using affinity purification, mass spectrometry, and phosphopeptide enri

131 rived complexes), which uses robust affinity purification-mass spectrometry methodology to elucidate

132 te (semi-)quantitative results from affinity purification-mass spectrometry, split-luciferase and yea

133 A repeat-binding protein, and using affinity purification-mass spectrometry, we show that Zfp106 inte

134 heir extracellular polymeric matrix clog the purification membranes and reduce their efficiency.

135 By direct analysis of water purification membranes using ambient ionization mass spe

136 mental friendly, efficient and promising dye-purification method and deserved further attention in fu

137 mportance of choosing a proper postsynthesis purification method for polyelectrolyte-wrapped NPs and

138 Hence, the BE-SEC based EV purification method represents an important methodologic

139 teractions, we establish a ribosome affinity purification method that unexpectedly identifies hundred

140 odifications to sequence, troubleshooting of purification method, and glycosylation differences are a

141 the Env sequence, the construct design, the purification method, and the producer cell type.

142 Membrane based purification methods are used extensively in water treat

143 However, simpler and more cost-effective purification methods need to be developed compared to ch

144 d blood cells, require efficient sorting and purification methods to remove components potentially ha

145 those obtained from conventional poly-A tail purification methods, indicating both enumerate the same

146 asing need for fast, reliable, and automated purification methods.

147 emain difficult to control, which means that purification must be used to obtain the desired products

148 olate's secondary metabolome resulted in the purification of a 22-mer peptaibol, gichigamin A (1).

149 d processes in the extraction and separation/purification of a large range of bioactive compounds (in

150 Here we describe the purification of a neurotoxin precursor processing protea

151 We recently described the purification of a novel trimer, TBCD*ARL2*beta-tubulin.

152 This work describes purification of a protease from the visceral mass of the

153 w reports that demonstrate the isolation and purification of a protein folding intermediate in native

154 Because the purification of a protein in native form is, in many sit

155 ed the task of optimizing the expression and purification of a recombinant form of FABP (Fh15).

156 on with mass spectrometry), for the affinity purification of a sequence-specific single-copy endogeno

157 e demonstrated its utility with one-step HHH purification of a wide range of traditionally challengin

158 Immunoaffinity purification of AtMC1-containing complexes led us to the

159 lotation and affinity chromatography for the purification of autophagosomes from cells that harbor an

160 ful for efficient prokaryotic production and purification of bioactive hFGF21.

161 Although purification of biotinylated molecules is highly efficie

162 The purification of biotinylated nuclei was redesigned by re

163 Purification of budding yeast condensin reveals that it

164 Purification of cell type-specific RNAs remains a signif

165 The preparation and purification of chemical dye- or reporter protein-antibo

166 is approach could lead to fast and automated purification of DNA nanostructures of various shapes and

167 NATMs offers transformative opportunities in purification of drugs, removal of residual reactants, bi

168 and strategies that allow the expression and purification of endotoxin-free antibody reagents suitabl

169 , we describe two methods for production and purification of filovirus glycoproteins in insect and ma

170 The co-purification of guanine nucleotide on the beta-tubulin i

171 port the heterologous expression and partial purification of His-tagged CesA5 from Physcomitrella pat

172 ltimode cation exchange chromatography based purification of human serum albumin.

173 d in vitro transcription allow synthesis and purification of hundreds of RNA mutants in a cost-effect

174 study describes the cloning, expression and purification of hypoxanthine-guanine-xanthine phosphorib

175 d in an operationally simple fashion without purification of intermediates.

176 deal molecular sieves for the separation and purification of light hydrocarbons are rarely realized.

177 system for the preparation, modification and purification of liposomes which offers lab-on-chip scale

178 s could enable development of new routes for purification of manufactured red blood cells.

179 nce of protein A chromatography resin during purification of monoclonal antibodies.

180 hesis of MPNs, (ii) conjugation with DNA and purification of monovalent MPNs, (iii) modular targeting

181 Hence, the purification of MPs from organic materials is crucial pr

182 traction (SPME) method was developed for the purification of mRNA (mRNA) from complex biological samp

183 reby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attr

184 modified cellulosic fibers is desirable for purification of natural water.

185 NT* also allows efficient expression and purification of non-transmembrane proteins, which are ot

186 Here, we integrate sucrose-gradient-assisted purification of nuclei with droplet microfluidics to dev

187 A full-scale purification of oligomeric proanthocyanidins (OPCs) deri

188 the direct saponification of the sample and purification of oxysterols by reversed phase C18-SPE fol

189 eport on the heterologous overexpression and purification of PfVIT, a vacuolar iron transporter homol

190 The purification of phlorotannins might expand their use as

191 The highest level of purification of phlorotannins was obtained with XAD-16N,

192 For the purification of phlorotannins, six resins (HP-20, SP-850

193 Following the purification of phytaspase from tomato leaves, two tomat

194 Purification of rare earth elements is challenging due t

195 mon tags used in the affinity chromatography purification of recombinant proteins.

196 Purification of resting CD4(+) T cells from whole PBMC i

197 their bound RNAs; partial RNA digestion and purification of RNA duplexes interacting with the specif

198 Here we show purification of SCF(Fbxo4) complexes results in the iden

199 to design catch-and-release systems for the purification of surface waters.

200 ethynyl group allows efficient detection and purification of tagged RNAs.

201 gh-copy expression plasmids enables the bulk purification of the aminoacyl-tRNA synthetases and trans

202 2,2-diphenyl-1-picrylhydrazyl (DPPH)-guided purification of the aqueous extract of Z. jujuba Mill.

203 In addition, purification of the crude product was tested.

204 Further purification of the dihydrazides 2, beyond simple isolat

205 We found that shear degradation hindered LC purification of the DNA nanoswitches, removing oligonucl

206 of NtRbcS-T in Chlamydomonas reinhardtii and purification of the full Rubisco complex showed that thi

207 This study reports the expression and purification of the full-length L protein of RABV and th

208 esterol analogue was required for the stable purification of the LAT1 with its chaperon CD98 (4F2hc,S

209 RNA editosome, we performed tandem affinity purification of the plastidial CHLOROPLAST BIOGENESIS 19

210 ur experiences with possible difficulties in purification of the proteins will facilitate other resea

211 We have combined biochemical purification of the Saccharomyces cerevisiae Mediator fr

212 Drying and purification of the sensing layer resulted in a well-def

213 Purification of the soluble cellulase activity from a 30

214 ing human embryonic kidney cells enabled the purification of the TBCD.ARL2.beta-tubulin trimer found

215 ic agents to treat S. aureus infections, and purification of the transmembrane transporter will enabl

216 have recently succeeded in the isolation and purification of these unique proteins, and the present s

217 nt here a hybridization capture strategy for purification of unspliced full-length HIV RNA-protein co

218 (PCGC) is the central technique used for the purification of volatile or semivolatile organic compoun

219 to very large constructs for expression and purification of whole pathways.

220 extraction solvent, the buffer salt, or the purification procedure, have been evaluated.

221 By establishing a purification procedure, performing further protein separ

222 rcomplex is absent or may be lost during the purification procedure.

223 rom bovine serum by an established four-step purification procedure.

224 Here, purification procedures were tested to eliminate anthocy

225 Difficulty in isolation and purification procedures, toxicity associated with the ac

226 uents prevent excess thionation, simplifying purification procedures.

227 ligands that must be removed via additional purification procedures.

228 on has emerged as a powerful tool to support purification process development.

229 Thus, the downstream purification process is designed to demonstrate robust r

230 ted at different steps and conditions of the purification process, including different culture durati

231 initial P. patens culture after a multistep purification process.

232 tocols because of the multistep construction/purification process.

233 pture of SO2 is of great significance in gas-purification processes including flue-gas desulfurizatio

234 Using two different galectin-3 affinity purification processes, we extracted four cell membrane

235 and may lead to innovative Bk separation and purification processes.

236 l monoclonal antibody (mAb) fermentation and purification processes.

237 tures of different particles, post-synthetic purification promises to provide insights into nanostruc

238 A basic enzymatic purification protocol (BEPP) proved to be efficient whil

239 biophysical techniques and an expression and purification protocol that generates clean, monomeric Ht

240 ammalian cell culture platform and a routine purification protocol.

241 lytical workflow both protein extraction and purification protocols were optimized and finally a sens

242 t culture durations, harvest procedures, and purification protocols were used to compare the methods.

243 tag sequences commonly added to proteins for purification purposes can distort the capture of the phy

244 nucleic acid extraction, concentration, and purification; refrigeration-free storage of reagents wit

245 econstitution from heterologous systems, and purification relies on scarce endogenous sources.

246 Nanoparticle tracking analysis and gradient purification revealed an increase in extracellular vesic

247 kinase (IKK) complex, and unbiased affinity purification revealed that MC005 interacts with the IKK

248 ermining the properties of proteins prior to purification saves time and labor.

249 ilization of an MsrQ-GFP fusion and set up a purification scheme allowing the production of pure MsrQ

250 arides are subjected to a greatly simplified purification scheme followed by structural remodeling us

251 uding nanomaterial synthesis or exfoliation, purification, separation, assembly, hybrid integration,

252 cellular carcinoma (HCC), the assay has high purification specificity (20 glycopeptides) with 2-fold

253 h step is >91% and it requires only a single purification step.

254 and 2-phenylethylamine, which eliminates the purification step.

255 The setup and purification steps are typically accomplished within 1-3

256 +) media without the need of progenitor cell-purification steps or support by a feeder cell layer.

257 f final (68)Ga-PSMA(HBED), making subsequent purification steps unnecessary.

258 ducing the effort required in the subsequent purification steps.

259 litates the protocol by eliminating two PAGE purification steps.

260 driven self-assembly with no chromatographic purification steps.

261 x (RSI) without need for separate work-up or purification steps.

262 Conventional biomolecule purification strategies achieve target capture using sol

263 rocess, it is critical to evaluate different purification strategies to obtain a clean final product

264 y(allylamine hydrochloride) (PAH), and three purification strategies were applied: (a) diafiltration

265 A detailed purification strategy for thaumatin is reported resultin

266 Pull down experiments completed by co-purification study prove that DauA and DctA interact phy

267 We anticipate that this expression/purification system and these RhoGC mutants will facilit

268 Here, we establish the first purification system for heteromeric cis-PT and show that

269 We present here an expression and purification system for isolation of the full-length Rho

270 efore, highly desirable to have an efficient purification system with a potential to meet the HHH ben

271 Portable purification systems are easy ways to obtain clean drink

272 nsideration when choosing a suitable protein purification tag.

273 elicolor lysate based on the tandem affinity purification (TAP).

274 This protocol obviates the purification techniques such as column chromatography fo

275 Water purification technologies such as microfiltration/ultraf

276 Using tandem affinity purification technology, we provide evidences that CDK20

277 After purification, the isoforms were physicochemically charac

278 Following purification, the RyR1 mutants G4934D, G4934K, and G4941

279 ni allow rapid bioconjugation and consequent purification through complexation with immobilized metal

280 onditions followed by column chromatographic purification to afford azaBODIPYs appended with one and

281 NT* enables transmembrane peptide purification to homogeneity without chromatography and m

282 tionation with translating ribosome affinity purification to identify ribosome-bound mRNA in processe

283 parations in applications ranging from water purification to petroleum refining, chemicals production

284 s generated by Translating Ribosome Affinity Purification (TRAP) and CREB-target gene repositories.

285 Translating ribosome affinity purification (TRAP) and in vitro luciferase reporter ass

286 We used translating ribosome affinity purification (TRAP) and RNA-seq to identify mistranslati

287 BAC aldh1l1-translational ribosome affinity purification (TRAP) mice (both sexes).

288 ne neurons for Translating Ribosome Affinity Purification(TRAP).

289 high binding capacity for the separation and purification under magnetic actuation of a wide range of

290 nthesized on a gram scale followed by simple purification via distillation.

291 e commonly fused to proteins to aid in their purification via metal affinity chromatography.

292 se neutrophils become easily activated after purification, we carried out ex vivo comparisons with ne

293 Using translating ribosomal affinity purification, we isolate cardiomyocyte-specific translat

294 This protocol is free from chromatographic purification, which makes it applicable for large-scale

295 n of histamine with 0.4M perchloric acid and purification with 1-butanol significantly shortened samp

296 ; it offers effective and rapid nanomedicine purification with high lipid recovery (> 98%) combined w

297 ed ER as confirmed by co-fluorescence and co-purification with known ER proteins.

298 a surface reporter for gentle and efficient purification with long-read single-molecule real-time se

299 Here, we use ChAP-MS (chromatin affinity purification with mass spectrometry), for the affinity p

300 Here, using co-purification with TOC159 from Arabidopsis, we discovered