ライフサイエンスコーパス: purified protein

1 d from assays using recombinantly generated, purified protein.

2 mammalian cells, and biophysical analysis of purified protein.

3 c mechanism has been hindered by the lack of purified protein.

4 in vivo to that previously observed for the purified protein.

5 ent from in vitro enzymatic assays using the purified protein.

6 een limited due to difficulties in obtaining purified protein.

7 xtensive biochemical characterization of the purified protein.

8 ractions in cell lysates and in solutions of purified protein.

9 netic deletion and enzymatic analysis of the purified protein.

10 tly analyze glycopeptides generated from the purified protein.

11 than that displayed in cell-free assays with purified protein.

12 r As(V) uptake was assessed with the His-tag purified protein.

13 previously been reconstituted in vitro with purified proteins.

14 arm axonemal dynein in motility assays using purified proteins.

15 eractions compared with methods that require purified proteins.

16 ng synthesis in a reticulocyte extract or as purified proteins.

17 zation of pAz on live cells, fixed cells and purified proteins.

18 d spores by comparison with known amounts of purified proteins.

19 of unlabeled ligand and typically use single purified proteins.

20 d by coimmunoprecipitation analyses and with purified proteins.

21 try performed on recombinantly expressed and purified proteins.

22 n of protein carbonylation in homogenates or purified proteins.

23 n protein folding in the ER and refolding of purified proteins.

24 eparations and x-ray crystallography data of purified proteins.

25 om brain tissue and shown to be direct using purified proteins.

26 , demonstrated by pulldown experiments using purified proteins.

27 process using only DNA templates instead of purified proteins.

28 studies of all the A3H variants using highly purified proteins.

29 for conferral of DNA-binding activity on the purified proteins.

30 idine HCl-induced unfolding titrations using purified proteins.

31 and enhanced stability and solubility of the purified proteins.

32 d ClyA-GFP fusion protein alone and equal to purified proteins absorbed to aluminum hydroxide, a stan

33 fs25) was expressed in Escherichia coli, and purified protein after simple oxidative refolding steps

34 allows a flow-based fractionation of highly purified protein aggregates and simultaneous measurement

35 nd C10F3.4, respectively) and found that the purified proteins also display GDP-hexose phosphorylase

36 Using purified proteins, an ATPase-defective Cdc6 mutant 'Cdc6

37 Mass spectrometry and purified protein analysis identified, mitochondrial HMG-

38 caftor stimulates the ATPase activity of the purified protein and can compete with the transport of t

39 rd protein immunoblotting procedures on both purified protein and crude nuclear extracts from HEK 293

40 d vitronectin, relative to non-risk, both in purified protein and in cellular models.

41 plates, requires sub-milligram quantities of purified protein and small quantities of detergents and

42 acterised for their Fab affinity against the purified protein and their Fc affinity to Protein A/G as

43 n vitro lysophospholipid binding assay using purified protein and transport assays with E. coli spher

44 This study demonstrates for purified protein and virus that the NA of the zoonotic H

45 Selected variants were then produced as purified proteins and characterized by surface plasmon r

46 T recruitment to viral assembly sites, using purified proteins and giant unilamellar vesicles.

47 ed by co-immunroprecipitation in vitro using purified proteins and in cells.

48 With purified proteins and in intact cells, our protein inter

49 ed with the isoforms both in vitro using the purified proteins and in vivo by fluorescence analysis i

50 Using purified proteins and isolated mitochondria, we show her

51 andidates in clinical development are highly purified proteins and peptides relying on adjuvants to e

52 ollowing a minimal systems approach, we used purified proteins and photolithographically patterned me

53 For purified proteins and protein complexes, our workflow us

54 Here, using purified proteins and the lipid bilayer system, we chara

55 ants for the reactions, reconstitutions with purified proteins and theoretical modeling to account fo

56 al genomics efforts, as most methods require purified proteins and/or are labor-intensive.

57 d assay, biochemical characterization of the purified protein, and in vivo complementation, demonstra

58 coli cells, characterizing properties of the purified proteins, and analysis of in vivo and in vitro

59 gns were successful in both lysates and with purified proteins, and that FlAsH binding was dependent

60 eraction between ROCK and SOX9 was tested on purified proteins, and was verified within a cellular co

61 mass spectrometry for identification of the purified proteins, and we identify novel gamma-secretase

62 After capture, the affinity-purified proteins are digested into peptides and compreh

63 have cloned, expressed, and used these three purified proteins as additives in synthetic magnetite pr

64 f Escherichia coli cells expressing LOA1 and purified proteins as enzyme sources.

65 sphorylation of RKIP at serine 153 utilizing purified proteins as well as in cells overexpressing RKI

66 Application of purified proteins at pH ranges in which PMEI inhibition

67 e rapid assembly of rods in vitro from these purified proteins at physiological concentration shows t

68 e yielded a large body of information on how purified proteins attain their native state when refolde

69 ry near the observed molecular weight of the purified protein based on gel electrophoresis.

70 release that is observed solely in NpHR via purified protein-based assays, demonstrating that indeed

71 econstituted this sophisticated machine with purified proteins, beginning with regulated CMG assembly

72 icating subgenomic replicon and analyzed the purified protein by mass spectrometry.

73 Analysis of the purified protein by size-exclusion chromatography sugges

74 We have characterized the anaerobically purified protein by UV-visible and EPR spectroscopies.

75 the complex with Rrp6-TAP, identified the co-purified proteins by mass spectrometry, and found karyop

76 detailed investigation of cell extracts and purified proteins by top-down MS, NMR spectroscopy, and

77 tro, complexes could be generated using four purified proteins-C3, factor B, factor D, and target pro

78 models have confirmed that brain lysates and purified protein can accelerate brain pathology in a man

79 ) as a model system, we demonstrate that the purified protein can be enzymatically modified with eith

80 process, based on Anfinsen's discovery that purified proteins can fold on their own after removal fr

81 The purified protein carrying the p.V742G variant showed red

82 richia coli from F(-) toxicity, and that the purified proteins catalyze transport of F(-) in liposome

83 Mass spectrometric analysis of the purified protein combined with isolation and sequencing

84 tandem affinity purification produces highly purified protein complexes at high concentrations in a h

85 We purified protein complexes containing biotin-tagged TR2/

86 g biologically relevant HIS-tag and FLAG-tag purified protein complexes, we demonstrate the separatio

87 ribe the reconstitution of E. coli FAS using purified protein components and report detailed kinetic

88 We have developed an assay, using purified protein components, in which inhibitors of any

89 e has never been reconstituted in vitro from purified protein components.

90 y involves assembly of multiple individually purified protein components.

91 nal flexibility measured experimentally with purified protein components.

92 , a majority of these techniques need to use purified protein, concentrated enough in the solution to

93 Intrinsic fluorescence spectroscopy of purified proteins confirmed a stronger binding reduction

94 Comparison with purified proteins confirmed that the PKCepsilon levels a

95 molybdenum-containing proteins, whereas four purified proteins contained sub-stoichiometric amounts o

96 The purified protein contains a mixture of zinc and iron and

97 In vitro kinetic analysis using purified protein demonstrated that the inhibition of 2-A

98 Immunoprecipitation and pulldown assays of purified proteins demonstrated a direct interaction betw

99 omparative in vitro binding experiments with purified proteins demonstrated an approximate 1:1 bindin

100 nd pulmonary trafficking or accumulation for purified protein deritative (PPD)-specific T effector ce

101 Purified protein derivative (PPD) is a widely used reage

102 s were cocultured with autologous T-Regs and purified protein derivative (PPD) preprimed T-Reg-deplet

103 d HIV-positive, TB-positive patients but not purified protein derivative (PPD)-negative or PPD-positi

104 Purified protein derivative (PPD)-specific immune recove

105 o Toll-like receptor (TLR)-2, -4 or -7/8, or purified protein derivative (PPD).

106 ntigen and 2 tuberculosis-specific antigens (purified protein derivative [PPD] and ESAT-6/CFP10), fol

107 However, 48 h after tuberculin purified protein derivative administration in the ipsila

108 Purified protein derivative ELISPOT responses increased

109 in skin test, and (4) Battey skin test using purified protein derivative from the Battey bacillus.

110 increased the in vitro cytokine responses to purified protein derivative of Mycobacterium tuberculosi

111 Polyclonal and purified protein derivative of tuburculin-specific T-cel

112 en both vaccines were administered together, purified protein derivative responses were enhanced in f

113 f IFN-gamma ELISPOT responses to recall Ags (purified protein derivative, Tetanus toxoid, or flu/EBV/

114 gamma release was significantly reduced when purified protein derivative- and Ag85B-specific CD4(+) T

115 The production of M. tuberculosis and purified protein derivative-induced IFN-gamma, TNF-alpha

116 absence of anti-LipC antibodies in sera from purified protein derivative-positive (PPD+) healthy subj

117 Immunization with BCG generated low purified protein derivative-specific CD4 T cell response

118 We observed that purified protein derivative-specific human CD4(+) T lymp

119 e stimulated with DEP and M. tuberculosis or purified protein derivative.

120 zyme-linked immunospot responses against BCG purified protein derivative.

121 In vitro analyses with purified proteins determined that the isolated Ras-GAP S

122 able to show that recombinantly produced and purified protein does not bind any known phytochrome chr

123 The purified protein exhibits a "hyper-helicase" unwinding a

124 Remarkably, cell extract and purified protein experiments show that MB induces disass

125 f some industrial lupin protein isolates and purified protein fractions were tested.

126 -supplied exogenously as genetic elements or purified protein fragments-had no significant catalytic

127 We show here, using purified proteins from budding yeast, that Dpb11 alone b

128 We show in this manuscript, using purified proteins from budding yeast, that Mcm10 directl

129 LCMS proteomic analysis of LAP-TAP-purified proteins from HeLa cells containing a tetracycl

130 Here, in a series of experiments using purified proteins from mammalian cells, bacteria, and a

131 reconstitute dynein plus-end transport using purified proteins from S. cerevisiae and dissect the mec

132 Here, we address this issue using purified proteins from Saccharomyces cerevisiae and a co

133 ction of X-ray fluorescence signals for 3879 purified proteins from several hundred different protein

134 Examples include purified proteins from the alpha-hemolysin pore from Sta

135 ion of DNA replication free in solution with purified proteins from the budding yeast Saccharomyces c

136 maturation was reconstituted in vitro using purified proteins from Thermococcus species 9 degrees N

137 onstituted system containing genomic DNA and purified proteins from yeast, Krietenstein et al. uncove

138 xogenous fatty acid supplementation, and the purified protein had less than 5% of the enzymatic activ

139 SDS-PAGE showed that the purified protein had molecular weight (24kDa) in concord

140 The purified protein has been crystallized in complex with t

141 ilarity with thiamin phosphate synthase, the purified protein has no thiamin phosphate synthase activ

142 X-ray structural analysis of the purified protein has revealed that the dimer is held tog

143 nstitution of diphthamide biosynthesis using purified proteins has not been reported.

144 Whereas MGs of various purified proteins have been probed to date, no data are

145 s isolated following in vitro interaction of purified proteins (hCtf4 plus the hCMG complex), coinfec

146 Using purified protein, here we show that the heterogeneous nu

147 specific interactions were detected even for purified proteins, highlighting the importance of correc

148 The purified proteins hydrolyzed the enamines/imines formed

149 mice and analytic size exclusion studies of purified proteins identify that interactions between cyc

150 The purified protein in a representative preparation contain

151 donuclease can be introduced into cells as a purified protein in complex with a single guide RNA (sgR

152 s the majority of spectroscopic studies, use purified protein in detergent micelles.

153 The purified protein in detergent showed a weak basal ATPase

154 limited due to inability of obtaining stable purified protein in sufficient quantities.

155 When recapitulated with purified protein in vitro, this modification completely

156 p, in part because it is difficult to obtain purified protein in well defined lipid systems.

157 Here we use purified proteins in a reconstituted system and in vivo

158 In conclusion, we utilized purified proteins in defined lipid membranes to quantita

159 refore, structural studies are restricted to purified proteins in vitro and these findings are extrap

160 s to complement activation peptide C5a using purified proteins in vitro, and by ex vivo depletion of

161 s and reconstituted assays of resection with purified proteins in vitro, we show that DNA-dependent p

162 nd to the sides of filaments faster than the purified proteins in vitro.

163 Reaction of the purified protein, in detergent, with the thiol-reactive

164 s of Pot1a-Tpt1-Pat1 complex formation using purified proteins indicated that Tpt1 interacts directly

165 Co-immunoprecipitation with purified proteins indicates that FANCI interacts with FA

166 The purified protein inhibited mitogen-activated proliferati

167 assay that reconstitutes this process using purified proteins instead of cytosol.

168 We show that the purified protein is a fully functional ion channel with

169 The expressed and purified protein is properly folded and has increased al

170 Because pegylation of purified proteins is commonly used as a method to increa

171 Obtaining highly purified proteins is essential to begin investigating th

172 PTO can be reconstituted in vitro with three purified proteins (KaiA, KaiB, and KaiC) and ATP.

173 assays using synthetic peptides showed that purified protein kinase A (PKA) could phosphorylate all

174 Both sites were phosphorylated by purified protein kinase A during in vitro assays.

175 utilizing traditional activity assays, where purified protein kinases are necessary.

176 nant rat DAT N-terminal peptide (NDAT) using purified protein kinases.

177 The purified protein likely exists in a monomer-dimer equili

178 Bioluminescence can be detected from the purified protein, live Drosophila Schneider 2 cells, and

179 lerated when compared to the dissociation of purified proteins measured in vitro.

180 The purified protein mediates Ca(2+)-dependent Cl(-) transpo

181 ractions cannot be studied using traditional purified protein methods, demonstrating the importance o

182 ter intranasal immunization of mice with the purified proteins mixed with the Th17 adjuvant curdlan,

183 n the minimal PURE system, which consists of purified proteins necessary for transcription and transl

184 obustly than their wild-type parent, and the purified protein of those mutants showed a decrease in c

185 was detected in both 1-tag cell lysates and purified proteins of R124C by the authors' custom-made a

186 CD45 from bound receptor-ligand pairs using purified proteins on model membranes.

187 Most previous studies on NM II used tissue-purified protein or expressed fragments of the molecule,

188 uctase (GR) inhibitors, directly against the purified proteins or in cell extracts.

189 f sugar nucleotidyltransferase activities of purified proteins or in cell lysates using a mass-differ

190 t screening allowing to obtain tens of mg of purified protein per liter of culture.

191 aled ERK1/2 as a positive regulator, whereas purified protein phosphatase 1 (PP1), dephosphorylated T

192 The purified protein possesses significant alpha-helical str

193 ry structure and oligomeric state using both purified protein preparations and in-cell NMR on E. coli

194 osomes containing functionally reconstituted purified protein provided strong evidence for active eff

195 of antibodies elicited by immunization with purified protein provides strong support for further eva

196 The SERS signal from the purified proteins provides basis spectra to analyze the

197 h different cysteine oxidation states of the purified proteins, providing a link between translation,

198 otrimer both in insect cell membranes and as purified protein reconstituted into a phospholipid bilay

199 Here we use purified protein reconstituted into liposomes to thoroug

200 ion of ETS2 and MESP1 or cell treatment with purified proteins reprograms fibroblasts into cardiac pr

201 ion of promoter nucleosome organization with purified proteins resolves this problem and is therefore

202 type biofilm morphology, indicating that the purified protein retained biological activity.

203 An initial spectroscopic characterization of purified protein revealed that the photocycle and the tr

204 Binding studies using purified proteins revealed that FX binds specifically (h

205 iated with cellular HSP70, and analyses with purified proteins revealed that SIP directly bound HSP70

206 ular enzyme-linked immunosorbent assay using purified proteins revealed that the binding affinity was

207 otypic analyses and biochemical studies with purified proteins revealed that the mono-haem c-type cyt

208 ST fusion proteins or GST-free counterparts, purified proteins revealed that the PX domain is suffici

209 In a purified assay using FVa R506Q/R679Q, purified protein S D95A was shown to have greatly reduce

210 The timescale from cell lysis to purified protein sample is 5-6 d.

211 Compared to purified protein samples, we show that pretreatment of c

212 subunits, and the UV-visible spectrum of the purified protein showed absorbance peaks at 380 and 460

213 e LXR4 was heterologously expressed, and the purified protein showed high specificity for L-xylo-3-he

214 Enzymatic assays using purified protein showed that unlike OdaA, which did not

215 The purified proteins showed similar kinetic behavior and co

216 In vitro binding assays using purified proteins showed strong affinity for the substra

217 ein-detergent complexes (PDCs) consisting of purified protein solubilized in a particular detergent.

218 The tests carried out using purified protein solution and clinical serum samples dep

219 hniques to measure polymer concentrations in purified protein solutions, but few are applicable in vi

220 agonize one another, and binding assays with purified proteins suggest that the association of RelA a

221 t plasmids containing pre-RCs assembled with purified proteins support complete and semi-conservative

222 Using purified proteins, surface plasmon resonance, and report

223 In a purified protein system, FXI is activated by beta-thromb

224 d SNAP-23 were tested for actin binding in a purified protein system, only syntaxin-4 associated dire

225 ecific pAz ligation and CuAAC on cells or on purified proteins takes 40 min-3 h.

226 igand screening or selection methods using a purified protein target.

227 ges for generating affinity reagents against purified protein targets, and we have now significantly

228 e utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leadi

229 Here, we purified proteins that bind to the 5' cap during the Ara

230 We show using purified proteins that HDHB physically interacts with th

231 We also demonstrate using purified proteins that MAP6-F binds directly to microtub

232 We show using purified proteins that Siah2 acts as an E3 ligase to dir

233 We further demonstrate, using purified protein, that the mutant protein is very stable

234 In addition to purified protein, the proposed immunosensor effectively

235 l recent efforts toward reconstituting, with purified proteins, the basic structural motifs that recu

236 umen formation can be rescued by addition of purified protein to knockdown cultures.

237 on studies in cells and on direct binding of purified protein to lipids.

238 We used purified proteins to assess interaction of the cytoplasm

239 a wide variety of preparations, ranging from purified proteins to reconstituted eukaryotic cell membr

240 Experimental spectroscopy studies with purified proteins validate our MD predictions and corrob

241 The purified protein was a dimer whose dimer interface invol

242 Further, glycation of this purified protein was carried out.

243 The expressed, purified protein was found to behave similarly to other

244 Purified protein was reconstituted into proteoliposomes

245 The purified protein was shown to be activated in a pH-depen

246 The purified protein was shown to be active with fatty acyl-

247 The purified protein was shown to be properly folded with st

248 The purified protein was then chemically modified with the b

249 This confirmed that the purified protein was trypsin.

250 as a lethal mutation in single copy, and the purified protein was unable to transfer biotin from enzy

251 Kinase activity of the affinity-purified proteins was assayed as autophosphorylation at

252 One of the purified proteins was identified as tenascin-R (TNR) by

253 Using a steady-state kinetics approach with purified proteins we demonstrate that eIF2B binds to eIF

254 Using purified proteins we found that arrestin-3 directly bind

255 Using purified proteins we show that arrestin-3 directly inter

256 Using purified proteins we show that DNA translocases, includi

257 Using purified protein, we report that human FANCA has intrins

258 Using purified proteins, we addressed the mechanistic defects.

259 Using purified proteins, we also found that PTP1B is relativel

260 Using purified proteins, we also show that the PARP1-XPC compl

261 By using purified proteins, we confirmed the direct interaction,

262 Using natively purified proteins, we demonstrate that the pyruvate and

263 Using purified proteins, we demonstrated that each VP24 binds

264 Using purified proteins, we determined that processing of the

265 Finally, using purified proteins, we find that Hrr25 phosphorylates the

266 Using purified proteins, we find that PLCbeta binds approximat

267 Finally, using purified proteins, we found that CsgE prohibited the sel

268 Using purified proteins, we found that DnaD inhibited the abil

269 Finally, using purified proteins, we found that MRN could stimulate bot

270 mass spectrometry and mutational analysis of purified proteins, we found that Set7/9 methylates the N

271 say and repair assays using cell extracts or purified proteins, we have shown that DNA double-strand

272 Using purified proteins, we observe a direct interaction betwe

273 Using purified proteins, we observed a direct interaction betw

274 When the pathway was reconstituted with purified proteins, we observed the formation of four acy

275 Using purified proteins, we reconstitute the regulation of the

276 ammalian cells, Xenopus nuclear extracts and purified proteins, we show that after DNA damage, PCNA l

277 Using purified proteins, we show that desmosomal cadherins and

278 Using purified proteins, we show that Sgs1, Top3, Rmi1, and re

279 Using purified proteins, we show that the replicative polymera

280 Using purified proteins, we showed that SATB1 interacts direct

281 ys with both mammalian cell-free extract and purified proteins, we unexpectedly discovered that lesio

282 rowth, and the biochemical properties of the purified protein were indistinguishable from those of Fa

283 The methyltransferase activities of the purified proteins were analyzed by methyl incorporation

284 The purified proteins were analyzed for the presence of lysi

285 Purified proteins were assessed for V3 loop binding usin

286 Purified proteins were evaluated for secondary structure

287 When the purified proteins were mixed, they became strongly but r

288 precursor protein, were identified, and the purified proteins were shown to inhibit the phagocytosis

289 Purified proteins were subjected to size exclusion chrom

290 stimulated A3G deamination activity when the purified proteins were used in in vitro reactions.

291 nteractions with signaling phospholipids use purified proteins, which do not take into account the ef

292 in detail and recapitulated it in vitro with purified proteins, which suggests direct interaction.

293 l Out Regulatory Elements (PORE), which uses purified protein with a stable genomic library to isolat

294 reagent, 2,2'-dithiodipyridine, to generate purified protein with its cysteine residues in the nativ

295 structure and biochemical properties of the purified protein with that of the recombinant human GCas

296 d to site-specifically label cell-surface or purified proteins with chemical probes in two steps: pro

297 We have combined X-ray crystallography of purified proteins with electron cryotomography of native

298 immunoblotting and by digesting recombinant/purified proteins with exogenous MMPs.

299 ing only five purification steps with a high purified protein yield (160 mg LTP1 and LTP1b from 200 g

300 d crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyc