ライフサイエンスコーパス: purify

1 with non-denaturing detergents, we affinity-purified 785 endogenously tagged CEPs and identified sta

2 Here, we purify a T4S system with VirD4 bound, define the biochem

3 HCAECs were stimulated with purified Aa serotype b lipopolysaccharide (LPS) (Aa-LPS)

4 10 weeks, followed by five oral dosing with purified AHC or ovalbumin on alternate days and continue

5 as well as toluene-(13)C7 in nitrogen and in purified air as drift gas.

6 of this equation with Blanc's law when using purified air as drift gas.

7 in healthy neutrophils after treatment with purified albumin from the study groups.

8 Here, we show that when highly purified, anatomically and phenotypically confirmed club

9 Here, we purified and assessed the origin, concentration and cont

10 nt molecules, vacuole membrane proteins were purified and binding to the surface molecules present in

11 We purified and compared the transcriptomes of pre-HSCs, HS

12 EVs including exosomes, and dense EVs, were purified and processed for proteomic profiling.

13 Here, we demonstrate that by combining purified and reconstituted in vitro translation, quantit

14 Here we show that the panda receptor, when purified and reconstituted into liposomes, forms an intr

15 Here we have employed purified and reconstituted P-glycoprotein to study its i

16 e generated against OTC molecule (anti-OTC), purified and the quality was assessed by enzyme linked i

17 n isoforms (rbeta) that we overexpressed and purified and to their natural counterparts in different

18 Here we report a simplified method to purify and amplify DNA from complex samples in a minimal

19 Here the authors purify and carryout a proteomics analysis of the protein

20 imeric proteins are easy to design, express, purify and crystallize.

21 metal affinity and immunospecific capture to purify and enrich multiple target biomarkers.

22 d in insect cells infected with baculovirus, purified, and crystallized.

23 Moreover, purified annexin A2 was able to stimulate the proliferat

24 Using purified antigen and specific tetramers, we showed that

25 PfVIT purified as a monomer and, as measured by intrinsic prot

26 The GTP-binding domain of CrSEPT purified as a nucleotide-free monomer that hydrolyzes GT

27 The 4 CD23 variants were purified as monomeric and structurally folded proteins,

28 MCLA-128 could be readily produced and purified at industrial scale with a standard mammalian c

29 The purified autophagosomes had double- or multiple-membrane

30 Purified BlArsM methylated both As(III) and methylarseni

31 e measured here the forces that ensembles of purified budding yeast kinesin-5 Cin8 produce in microtu

32 ans NRRL Y-2311-1 produced on wheat bran was purified by a single-step chromatographic procedure.

33 malian vectors, secreted into culture media, purified by affinity chromatography and characterized by

34 alt form, could be conveniently isolated and purified by crystallization.

35 mma (IFNG)-producing and non-producing cells purified by flow cytometry.

36 xtracted with 80% (v/v) aqueous methanol and purified by liquid chromatography.

37 An IgG1 monoclonal antibody (mAb) purified by Protein A column elution, cation exchange ch

38 Bovine hemolysate was purified by size exclusion chromatography, and one high

39 idge Extraction (LLCE), the FFAs extract was purified by Solid Phase Extraction (SPE), methylated by

40 EVs have traditionally been purified by ultracentrifugation (UC), however UC has lim

41 Addition of purified C3 cleavage products to cultured neurons sugges

42 C or monocytes were directly stimulated with purified C3a or C5a.

43 presence of intact human serum complement or purified C3a or C5a.

44 Purified CA polymers are sufficient to promote longevity

45 coupling the systematic genomic analysis of purified cancer cells in distinct malignant states from

46 ve regimen, followed by adoptive transfer of purified CD4(+) T cells, retrovirally transduced with MA

47 heral blood mononuclear cells (PBMC), and in purified cell subsets from affected and unaffected famil

48 Flow cytometry and adoptive transfer of purified cells show that antibiotic-induced suppression

49 nI activity, this study next determined that purified chymotrypsin or mouse intestinal fluids can als

50 ent study for the first time isolated highly purified club cells for in vitro study and demonstrated

51 ated lysine acetylation, and the presence of purified CobB protects TopA from inactivation by such no

52 The stability and high yield of the purified complex should allow for further efforts toward

53 Using purified components, and Loqs-PD truncations, we provide

54 In addition to affinity measurements with purified components, Kd values for unpurified proteins i

55 The purified compound showed similar properties compared wit

56 Component sugar analysis of purified CPS-PG identified only CPS and PG sugars in the

57 In vitro reconstitution experiments with purified cyt b6f and recombinant Stt7 kinase domain show

58 lated chromatin segments (PICh) technique to purify deprotected telomeres, PHF11 was enriched as cell

59 ces have provided a framework for rationally purifying, describing, and manipulating macrophage polar

60 Purified dimeric species of 1-4DeltaMBD-7B were characte

61 Recombinantly expressed, purified DmGSTE6 produces predominantly 2-glutathionyl-4

62 In recalcified PFP, purified DNA triggered contact-dependent thrombin genera

63 Here, we show that purified Drosophila Fhod and human FHOD1 both accelerate

64 tal-benign bio-measure that could be used to purify dye-contaminated wastewater.

65 ite bread of adding crude (E1) and partially purified (E2) arabinoxylans (AX) from wheat bran to part

66 Purified EMC in synthetic liposomes catalyzed the insert

67 tute a dynamic tubular membrane network with purified endoplasmic reticulum proteins.

68 nation have relied on in vitro systems using purified enzymes and were limited by availability of deu

69 g Ir(Me)-PIX in cell lysates, rather than as purified enzymes.

70 Finally, we show that V. fischeri, purified EroS, and other bacterial chondroitin lyases in

71 IN10-dependent phosphorylation of WRI1 using purified Escherichia coli-expressed components.

72 Moreover, purified EVs as well as the MASP SP inhibit the action o

73 ze exclusion chromatography (BE-SEC) columns purify EVs with high yield (recovery 80%) in a time-ef

74 Circuitoids of purified excitatory interneurons were sufficient to gene

75 Whereas purified fibrinogen from healthy controls displayed no p

76 contributed to the binding of host cells and purified fibronectin and laminin, as well as Yop deliver

77 We developed a purified formalin-inactivated Zika virus vaccine (ZPIV)

78 s along culture growth, and their profile in purified fractions (bodies and faeces) and international

79 s a protease with high activity in the cold, purified from a psychrophilic microorganism.

80 phosphorylation sites in the NaV1.5 proteins purified from adult WT and failing CaMKIIdeltac-overexpr

81 d a quantitative proteomics analysis of eHAV purified from cell-culture supernatant fluids by isopycn

82 significantly more HLA-DR and KLH than those purified from control MoDC, and KLH was concentrated spe

83 We studied PSII core complexes purified from D1-N87A and D1-N87D variant strains to pro

84 cytoplasmic C terminus in cultured cells and purified from Escherichia coli The alpha-helical motif w

85 Furthermore, monocytes purified from ET(flox/flox);Tie2-Cre( + ) mice, which co

86 Strikingly, exosomes containing vsRNAs, purified from haemolymph of infected flies, confer passi

87 uronan/Pentraxin 3 (HC-HA/PTX3) is a complex purified from human amniotic membrane (AM) and known to

88 was assessed in innate lymphoid cell subsets purified from human PBMCs and mouse lung ILC2s.

89 DWV was purified from infected adult worker bees to pursue bioch

90 Here, we profiled Schwann cells acutely purified from intact nerves and from the wound and dista

91 uentially passaged in BALB/c mice and plaque-purified from lung samples.

92 Anti-LSA3-C-specific antibody purified from Malian adult plasmas showed GIA activity,

93 )) and 4 IgG2c nonadjuvanted, isologous mAbs purified from serum-free medium.

94 oreactor treatment, and the C15H8O2 compound purified from soil extracts was determined to be genotox

95 Ms in the chloroplast ATP synthase (cATPase) purified from spinach leaves.

96 ntal assays performed on OL progenitor cells purified from Thap1 null mice.

97 Biochemical analysis of WRN protein purified from TT lymphoblast cell lines confirmed that t

98 gial cells as well as in glomeruli that were purified from type I and type II diabetic mice.

99 hycobilisomes with the PSI-LHCI supercomplex purified from various light conditions, suggesting that

100 stability of VVTL1, a Thaumatin-like protein purified from wine, was analyzed by Synchrotron Radiatio

101 Lumacaftor partially stabilized purified full-length F508del-CFTR and slightly delayed d

102 Here, we expressed and purified full-length porcine INSL3 (pINSL3) using a silk

103 Purified fusion protein was encapsulate into MNPs with a

104 TRPM3 co-immunoprecipitated with Gbeta, and purified Gbetagamma proteins applied to excised inside-o

105 lysis of glomerular cells from magnetic bead-purified glomeruli have identified glomerulus-infiltrati

106 s in brain microsomes from wild-type mice or purified GPI-anchorless amyloid fibrils).

107 icacy of KDM1A targeting drugs was tested on purified GSCs isolated from established and patient-deri

108 demonstrated that administration of a highly purified (>95%) short-chain galactooligosaccharide (GOS)

109 h-affinity magnetic immunocapture to rapidly purify HA-tagged mitochondria from homogenized mammalian

110 Purified Hb auto-oxidized to metHb more rapidly than Hb

111 microscale immunoaffinity chromatography to purify HBeAg from individual patient plasmas.

112 Our global proteomic analysis of purified HCs extends the existing HC transcriptome, reve

113 ontrol breeder chow diet (25% fat) or a semi-purified HFD (45% fat) 4 weeks prior to mating with WT/K

114 We produced and purified HIV-1 particles from SERINC5293T cells with ver

115 The purified hNGF was highly functional as it displayed the

116 ntifibrotic effects of repeated infusions of purified HSCs.

117 On the contrary, we found that recombinant purified human apurinic/apyrimidinic endonuclease-1 (APE

118 A test dataset was generated using highly purified human classical and nonclassical monocyte subse

119 Although the [2Fe-2S] cluster in purified human mitoNEET and Miner1 fails to bind nitric

120 changes in microRNA expression occurring in purified human naive CD4 T cells in response to TCR stim

121 human skin cryosections were incubated with purified human peripheral blood eosinophils with or with

122 Purified human telomerase catalyzes processive repeat sy

123 anscription following injection of different purified IFNs.

124 tamine from human mast cells sensitized with purified IgE of patients with CSU but not control subjec

125 Mice injected with purified IgG from rabbit serum that was taken before imm

126 Intravenous immunoglobulin (IVIG) are purified IgG preparations made from the pooled plasma fr

127 growth of cells that do not secrete IL-9, as purified IL-9 secretors demonstrate the same loss of IL-

128 ed, ex vivo Ag-driven molecular profiling of purified immune cells to detect predictive biomarkers of

129 atically performed microchemotaxis assays on purified immune subsets including human pan-T cells, CD4

130 CopA from Escherichia coli was expressed and purified in fully functional form and demonstrated to bi

131 n that it can be produced quickly and easily purified in large quantities from Nicotiana benthamiana

132 Here we show that transfection of purified in vitro generated circRNA into mammalian cells

133 tion of culture medium containing Mcc(ia) or purified in vitro-generated Mcc(ia) into the culture ind

134 Comparison of the profiles of purified iPSC-derived DaNs derived from Parkinson's dise

135 at 40% amplitude increased the solubility of purified kafirin from 6.5mug/mL to 173.3mug/mL.

136 Synthetic or honey-purified leptosperin not only displayed an identical flu

137 Purified lipase had Michaelis-Menten constant (Km) and c

138 Purified LLMs invaded erythrocytes by an increase of 10-

139 The electrochemical responses of purified low and high density lipoproteins (LDL and HDL,

140 Transmigration of purified lymphocytes was dependent on ADAM10 regulation

141 the range of low ppm to over 100 ppm in the purified mAb sample.

142 mediated by hemozoin, because incubation of purified malaria pigment with DNase abrogated IFN-beta i

143 e used a reconstitution strategy with highly purified mammalian GIRK2 channels incorporated into lipo

144 Finally, we use the purified membrane protein as an antigen to discover nati

145 A plaque-purified MERSMA clone caused weight loss and fatal infec

146 ned physical and chemical characteristics of purified meta-Cresol Purple (mCP) pH indicator dye suita

147 Here we present gene expression profiles of purified microglia isolated at autopsy from the parietal

148 Cytochrome c oxidase (COX) was initially purified more than 70 years ago.

149 Lipid binding by purified MpPR-1 occurs with micromolar affinity and is s

150 Furthermore, affinity-purified MRP ribonucleoprotein (RNP) from HeLa cells cle

151 RNA-dependent interaction with chromatin, we purified native nucleosomes from mouse ES cells and dete

152 Although the effects of purified NET components including DNA, histone proteins,

153 ARE-containing planar-supported bilayers and purified neuroendocrine dense core vesicles (DCVs) as fu

154 found a difference in activation state, with purified neutrophils showing signs of increased reactivi

155 The assay successfully detected purified norovirus RNA from multiple patient outbreak is

156 P-X also cross-linked to purified NPC1 that was incorporated into lipid bilayer n

157 Purified NRP1(+) cells from BAL had even greater functio

158 NSCs, and concentrating and transporting the purified NSCs to the surgery room, could be integrated a

159 rotid administration of MSC-oHSV, but not of purified oHSV alone, effectively tracks metastatic tumor

160 nctional aspects of ligand interactions with purified P-gp and other ATP-binding cassette transporter

161 al protein A elution starting from either a) purified parental mAbs, b) in-supernatant crossed parent

162 To overcome this limitation, purified patient-specific Tregs are expanded ex vivo and

163 We show that purified PBP2a can cross-link glycan strands bearing pen

164 We performed RNA-seq on purified peripheral afferent neurons, but found no strik

165 Wild-type T. denticola and the purified PF triggered activation of NF-kappaB through TL

166 In addition, highly purified PF were able to dose dependently stimulate cyto

167 The purified phosphorylated protein bound to a phosphotyrosi

168 aride standards and to de novo sequencing of purified plant metabolite glycoconjugates, showing that

169 However, the lyase activity of purified pol gamma was weak against the 5'-AMP-dRP block

170 Purified PP1c bound to recombinant Gbeta1-GST protein, a

171 analysis showed directly binds and inhibits purified ppGalNAc-T3 with no detectable activity against

172 -alpha receptor 1-deficient mice, as well as purified primary ILC2s, to reach our objective.

173 e for the first time that TGFbeta can induce purified primary lens epithelial cells within the same c

174 e presence of nonphysiological aggregates in purified properdin preparations and experimental models

175 rotein ubiquitination was prevented, or when purified proteasomes hydrolyzed the associated ubiquitin

176 caftor stimulates the ATPase activity of the purified protein and can compete with the transport of t

177 The purified protein carrying the p.V742G variant showed red

178 nal flexibility measured experimentally with purified protein components.

179 donuclease can be introduced into cells as a purified protein in complex with a single guide RNA (sgR

180 limited due to inability of obtaining stable purified protein in sufficient quantities.

181 When recapitulated with purified protein in vitro, this modification completely

182 The tests carried out using purified protein solution and clinical serum samples dep

183 The purified protein was a dimer whose dimer interface invol

184 Reaction of the purified protein, in detergent, with the thiol-reactive

185 d from assays using recombinantly generated, purified protein.

186 Application of purified proteins at pH ranges in which PMEI inhibition

187 detailed investigation of cell extracts and purified proteins by top-down MS, NMR spectroscopy, and

188 process, based on Anfinsen's discovery that purified proteins can fold on their own after removal fr

189 CD45 from bound receptor-ligand pairs using purified proteins on model membranes.

190 The SERS signal from the purified proteins provides basis spectra to analyze the

191 Experimental spectroscopy studies with purified proteins validate our MD predictions and corrob

192 stimulated A3G deamination activity when the purified proteins were used in in vitro reactions.

193 Using purified proteins, we reconstitute the regulation of the

194 Using purified proteins, we show that the replicative polymera

195 try performed on recombinantly expressed and purified proteins.

196 As purified PvdO was inactive, additional factors are requi

197 Mice gavaged with purified R gnavus developed airway hyper-responsiveness

198 trafast transient absorption spectroscopy of purified RC/YFP complexes show that the YFP-RC intermole

199 Purified recombinant 3MST and lysates of COS cells expre

200 ynthetic microalga Chlamydomonas reinhardtii Purified recombinant ADHE catalyzed the reversible NADH-

201 ed and recombinant lipoprotein(a) as well as purified recombinant apo(a) variants were able to specif

202 Purified recombinant apo-DnrF bound its target promoter

203 nsic fluorescence to study the activities of purified recombinant ApoL1.

204 The purified recombinant CrACX2 expressed in Escherichia col

205 In vitro experiments with purified recombinant CYP46A1 indicated that CYP46A1 is a

206 Here, we purified recombinant DDX43 protein to near homogeneity a

207 Here, we purified recombinant FA core complex to reveal the funct

208 more, dose-dependent binding of human VWF to purified recombinant human MGL was confirmed using surfa

209 inetics revealed that the Km and Vmax of the purified recombinant MzASMT9 protein for melatonin synth

210 We also prepared a specific Ab against the purified recombinant NC1 domain peptide.

211 Visible-absorption spectra of purified recombinant TcAPx-CcP after H2O2 reaction denot

212 s36115365-C, further supported by binding of purified recombinant ZNF148.

213 ls and animals, we developed preparations of purified, recombinant, murine Arl13b protein.

214 5% kcal from fat enriched in palmitate) or a purified regular diet (16.9% kcal from fat) for 3, 6, 9

215 Moreover, the addition of purified RelE to cell lysates shows promise as a method

216 In conclusion, purified REP with efficient bioavailability may be safe

217 ected boa constrictors and ball pythons with purified reptarenavirus.

218 alization immediately after axonal injury in purified retinal ganglion cells.

219 Here, we evaluated the safety and effects of purified rice endosperm protein (REP), which contains le

220 The released, labeled, and purified RNA is detected by a sensitive and compact fluo

221 embrane association of HIV-1 Gag, as well as purified Rous sarcoma virus (RSV) MA and Gag, depends st

222 In vitro, purified RPA directly enhances the association of RNaseH

223 y and a lower maximal turnover rate than the purified sarco(endo)plasmic reticulum Ca(2+)-ATPase (SER

224 lar vesicles (GUVs) and smaller liposomes or purified secretory granules with high temporal and spati

225 n years old), only three showed evidence for purifying selection (omega </= 0.14).

226 he ND2 and ND5 genes against a background of purifying selection across the mitogenome.

227 lated bacterial and archaeal genomes reveals purifying selection affecting AUG codons.

228 is-regulatory variation (i) are under weaker purifying selection and (ii) undergo less frequent posit

229 metric inheritance maintains the efficacy of purifying selection at the level of the cell, the absenc

230 Finding a signature of purifying selection in a gene is usually interpreted as

231 ing protein-coding genes are typically under purifying selection in full MHs.

232 synonymous variant supported the presence of purifying selection in Koreans.

233 n evolution revealed a significant effect of purifying selection in the concatenated protein-coding g

234 ent drug pressure bears a clear signature of purifying selection leading to apparent genetic stabilit

235 cause, multiple lines of evidence support a purifying selection model above a mutational bias explan

236 es, such CGIs were found to be under greater purifying selection relative to CGIs upstream of silence

237 Strikingly, a clear signal of purifying selection remains even when all these major ca

238 ry variation in C. grandiflora is under weak purifying selection, and that gene-specific functional c

239 es, HLA haplotype diversity patterns suggest purifying selection, as certain HLA allele combinations

240 e-onset effects are kept at low frequency by purifying selection.

241 tations, we show that prfA and hly are under purifying selection.

242 l start codon that is actively maintained by purifying selection.

243 ed with efficient covalent immobilization of purified single-stranded DNA probe oligomers on cleaned

244 By reconstituting purified Smoothened in vitro, we show that cholesterol w

245 tanding optical and electronic properties of purified, solution-processed semiconducting (6,5) single

246 We then purified Spc29 and reconstituted the ternary Mps2-Bbp1-S

247 The purified SPCA1a displays a higher apparent Ca(2+) affini

248 In purified stimulated human B cells, 9cRA markedly reduced

249 Our analyses revealed that purified StmPr1 behaves more similarly to subtilisin tha

250 We also determined that purified StmPr1 likely induces cell rounding and detachm

251 KMD formulas relatively more abundant in the purified subfraction where Pu had been observed than in

252 Purified SUV39H1 directly binds nucleic acids through it

253 ene expression analysis using low amounts of purified, synthetic DNA in well characterized samples un

254 Strikingly, signaling downstream of PD-1 in purified T cell subsets did not correlate with PD-1 surf

255 ation assay containing human cell lysate and purified target mRNA fused to a reporter was used to ide

256 including micro-RNA switch technologies that purify target cell types are also outlined.

257 We previously purified TBCD from bovine tissues and showed that it tig

258 Fluorescence-activated cell sorting (FACS)-purified Tbx18-expressing cells behaved as MSCs in vitro

259 The tomato-paste purified TCMPs retained the resistance to gastrointestin

260 strain DSMZ 1677(T) in Escherichia coli and purified the enzyme under anoxic conditions.

261 Here, we expressed and purified the luminal domain of Saccharomyces cerevisiae

262 Here, we have purified the major E3 ubiquitin ligases from human cells

263 e induced the differentiation of spheres and purified the myofibroblast-like cells.

264 protein cofactor (P) in Escherichia coli and purified the resulting proteins by affinity and size exc

265 Using a chemical biology approach, we purified the sigma2 receptor from tissue, revealing its

266 of individual isoforms, we overexpressed and purified the three full-length human isoforms from suspe

267 activity of TCMPs, we developed a method for purifying the proteins from tomato paste.

268 Once purified, the transcriptomic profiles of iPSC-derived Da

269 arms were assembled to form BsAbs that were purified through protein A affinity chromatography to de

270 n to modulate TLR activation by synthetic or purified TLR ligands and may play an important role in t

271 MBC4 and the parental allergens were purified to homogeneity.

272 The relaxation activity of purified TopA is decreased by in vitro non-enzymatic ace

273 cholate and chenodeoxycholate interact with purified ToxRp and destabilize it.

274 We purified trace-active derivatives of kynurenine and iden

275 Here we show that purified transposase binds specifically to the Muta1 end

276 The resultant purified triacylglycerols accomplished with the oxidativ

277 ilding upon previous work demonstrating that purified trypsin can activate NanI activity, this study

278 an inexpensive, simple and fast procedure to purify trypsin based on affinity binding with ferromagne

279 8)F(-) with SO3-pyridine complex in MeCN and purified using alumina and quaternary methyl ammonium so

280 irmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography.

281 Addition of purified Usp14 to the WT and Usp14-defficient proteasome

282 t H. pylori strains, treatment of cells with purified VacA proteins and infection of a mouse model, w

283 Using a PV replicon and purified virion RNA, we also show that eIF4E promotes th

284 Proteomic analysis of purified virions suggests that packaging of antirestrict

285 induced aggregation and the stabilization of purified VLPs.

286 Using cell-free reconstitution with purified Vps13p, we show that Vps13p is directly require

287 n be restored by exposure to WT plasma or to purified VWF depending on the VWF integrin binding site.

288 l-free process produced on average 1.5 mg of purified, water-soluble mMOMP-tNLP complex in a 1-ml cel

289 Our proteomic analysis of purified whole secretory granules or secretory granule m

290 heterodimers similar to those formed by GNA-purified wild-type (WT) E1E2 and exhibited nearly identi

291 Here, by examining purified wild-type and mutant BRCA1-BARD1, we show that

292 Compared with purified wild-type CFTR, the full-length F508del-CFTR wa

293 used calixarene detergent to solubilize and purify wild-type non-aggregated and homogenous KCC2.

294 ied an essential light chain (PfELC) that co-purified with PfMyoA isolated from parasite lysates.

295 mass spectrometry we show that minispryn co-purifies with myospryn and the major cardiac ryanodine r

296 ever, we detected cellular nucleases that co-purify with Cas10-Csm, and show that Csm5 regulates thei

297 c rPfs25/8(CDeltaS) was expressed and easily purified, with the Pfs25 domain bearing the proper confo

298 Purified Wogonin mimicked the effects of F4 in IL-1beta-

299 The Km and Vmax values for purified Xyl2 were 9.6mg/mL and 28.57mumol/min/mg, respe

300 chemical characterization of the recombinant purified Ydr109c and FGGY proteins showed a clear substr