ライフサイエンスコーパス: pyridoxal phosphate

1 by catalyzing incorporation of its cofactor, pyridoxal phosphate.

2 do not form heterotetramers, and do not bind pyridoxal phosphate.

3 e pyrroline ring with the aldehyde carbon of pyridoxal phosphate.

4 nting stable formation of a Schiff base with pyridoxal phosphate.

5 idue interacts with the pyridine nitrogen of pyridoxal phosphate.

6 s form external aldimines with the coenzyme, pyridoxal phosphate.

7 The enzyme contains covalently bound pyridoxal phosphate.

8 to stabilize the 3'O- functionality of bound pyridoxal phosphate.

9 ition about 8 angstrom from the phosphate of pyridoxal phosphate.

10 nd the nonselective P2 receptor antagonists, pyridoxal phosphate 6-azophenyl-2',4'-disulfonic acid an

11 rents were blocked by 100 microm suramin and pyridoxal phosphate 6-azophenyl-2',4'-disulfonic acid.

12 The P2Y receptor antagonists suramin and pyridoxal phosphate 6-azophenyl-2'-4'-disulfonic acid bo

13 Pyridoxal phosphate 6-azophenyl-2,4'-disulfonic acid, an

14 ythroidine and reduced by the P2X antagonist pyridoxal phosphate-6-azo (benzene-2,4-disulfonic acid (

15 r ATP, and by blocking P2 purinoceptors with pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid) t

16 PPADS [pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid)],

17 ts were potentiated by low concentrations of pyridoxal phosphate-6-azophenyl-2', 4'-disulfonic acid a

18 Suramin and pyridoxal phosphate-6-azophenyl-2', 4'-disulfonic acid w

19 osine-3', 5'-bisphosphate (K(i)=900 nM), and pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (K

20 ramin (100 micromol/L) or the P2X antagonist pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (P

21 as blunted after the P2X receptor antagonist pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (P

22 ed in low divalent cation medium, blocked by pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (P

23 e) inhibition by Brilliant Blue G, Cu2+, and pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid te

24 (1 microM), but persisted in the presence of pyridoxal phosphate-6-azophenyl-2',4'-disulphonic acid (

25 ion of the P2 purinergic receptor antagonist pyridoxal phosphate-6-azophenyl-2',4'-disulphonic acid (

26 5-na phthalene-trisulphonic acid) and PPADS (pyridoxal phosphate-6-azophenyl-2',4'-disulphonic acid).

27 Hindlimb intra-arterial infusion of pyridoxal phosphate-6-azophenyl-2,4-disulfonic acid (PPA

28 Pyridoxal-phosphate-6-azophenyl-,2',4'-disulphonic acid

29 eptor antagonists periodate-oxidized ATP and pyridoxal-phosphate-6-azophenyl-2', 4'-disulfonic acid,

30 adult rats show that bilateral injections of pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS

31 blocked by P2Y receptor antagonists suramin, pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS

32 The ENPP1 inhibitor pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS

33 s with the broad-spectrum antagonists PPADS (pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate) or RB

34 by the antagonists suramin (300 microM) and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (3

35 e sensitivity to the antagonists suramin and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (P

36 d by a pyridoxal-phosphate-based antagonist (pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid) o

37 was blocked by the P(2) receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

38 P2 receptor antagonists suramin (0.02 M) and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

39 by 97 +/- 2% by the P2X receptor antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

40 blocked by the broad P2 receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

41 blocked by the broad P2 receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

42 ed to MA; (iii) the P2X receptor antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

43 yrrhetinic acid, blocking ATP receptors with pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (

44 Reactive Blue 2 and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid i

45 e or no effect on sensitivity to suramin and pyridoxal-phosphate-6-azophenyl-2,4-disulfonate in chime

46 r, which is inhibited by NF449, suramin, and pyridoxal-phosphate-6-azophenyl-2,4-disulfonate, with re

47 ), which forms an internal aldimine with the pyridoxal phosphate and catalyzes the abstraction of the

48 unusual in its dependence on two cofactors: pyridoxal phosphate and heme.

49 a-His(86) plays a structural role in binding pyridoxal phosphate and in stabilizing the correct orien

50 The presence of pyridoxal phosphate and pyridoxal as ligands was confirm

51 The results of this screen identified pyridoxal phosphate and pyridoxamine phosphate as equili

52 al to a compound previously synthesized from pyridoxal phosphate and pyruvate.

53 s of very slow biological reactions, notably pyridoxal phosphate and the ceric ion, are shown to meet

54 ny chromogenic cofactor, and the presence of pyridoxal phosphate and the mechanistically related pyru

55 interaction of His(86) with the phosphate of pyridoxal phosphate and with Lys(87) lowers the pK(a) of

56 Unexpectedly, the double bond linking the pyridoxal-phosphate and benzoate moieties was reduced by

57 NAD, pyridoxal phosphate, and coumermycin A1, which exhibit n

58 en fasting plasma levels of vitamin B(6), as pyridoxal phosphate, and subsequent myocardial infarctio

59 xygen-sensitive and biotin-, ATP-, thiamin-, pyridoxal phosphate-, and metal-ion-independent, reversi

60 The pyridoxal phosphates appear to be primarily present as g

61 This E. coli protein contains pyridoxal phosphate as a cofactor and catalyzes the remo

62 as a molecular weight of 45 kDa and contains pyridoxal phosphate as a cofactor.

63 hould be measured, and therapeutic trials of pyridoxal phosphate as well as pyridoxine should be cons

64 The protein contained a highly conserved pyridoxal phosphate attachment site in the C-terminal do

65 ed the serine/threonine dehydratase types of pyridoxal-phosphate attachment site.

66 LPS-induced iNOS expression is mimicked by a pyridoxal-phosphate-based antagonist (pyridoxal-phosphat

67 isomal aggregation (Gly41Arg), inhibition of pyridoxal phosphate binding and loss of catalytic activi

68 cement of His(86) by leucine (H86L) weakened pyridoxal phosphate binding approximately 20-fold and ab

69 DhpH is a multidomain protein, in which a pyridoxal phosphate binding domain is fused to an N-acet

70 Mutations in the pyridoxal phosphate binding site of the tryptophan synth

71 at several key residues directly involved in pyridoxal phosphate binding were strictly conserved.

72 acid sequence of CobD identified a consensus pyridoxal phosphate-binding motif.

73 o acid sequence of CGS1 contains a consensus pyridoxal phosphate-binding site and is similar to MetB

74 amer comprising two obligate dimers and four pyridoxal phosphate-bound active sites.

75 8 to participate in nucleophilic attack of a pyridoxal phosphate-bound cysteine substrate.

76 studies of the enzyme because the different pyridoxal phosphate-bound intermediates can be followed

77 lyzes the second step in the biosynthesis of pyridoxal phosphate by oxidizing 4-phospho-d-erythronate

78 tational study of nonenzymatic and enzymatic pyridoxal phosphate-catalyzed decarboxylation of 2-amino

79 ne forms a stable external aldimine with the pyridoxal phosphate coenzyme at the active site of the b

80 tated by the spectroscopic properties of the pyridoxal phosphate coenzyme that forms a series of inte

81 tated by the spectroscopic properties of the pyridoxal phosphate coenzyme.

82 e provides information on the binding of the pyridoxal phosphate cofactor as well as on amino acid re

83 ocated on a helix that connects the heme and pyridoxal phosphate cofactor domains.

84 ases via reactions of the compounds with the pyridoxal phosphate cofactor forming an irreversible add

85 interacts with the pyridine nitrogen of the pyridoxal phosphate cofactor from a neutral Ser (beta-Se

86 L-Allothreonine reacts with the pyridoxal phosphate cofactor to form a stable 3-methyl a

87 ing strain of E. coli and shown to contain a pyridoxal phosphate cofactor, as judged by ultraviolet/v

88 s328 is positioned greater than 17A from the pyridoxal phosphate cofactor, suggesting that a large co

89 ond of the serine side-chain mediated by the pyridoxal phosphate cofactor.

90 PtaA is homodimeric and contains a pyridoxal phosphate cofactor.

91 of Cys328 to within approximately 3A of the pyridoxal phosphate cofactor.

92 A of the glycine alpha-carbon of the glycine-pyridoxal phosphate complex; the complex appears to be p

93 Folate, vitamin B12, and pyridoxal phosphate concentrations were also determined.

94 Residual activity of K165M is insensitive to pyridoxal phosphate, confirming K165 as the target of th

95 r and UV-visible spectra characteristic of a pyridoxal phosphate containing enzyme.

96 Like NifS, IscS is a pyridoxal-phosphate containing homodimer.

97 We recently discovered that the pyridoxal phosphate-containing enzyme PvdN is responsibl

98 NIFS protein from Azobacter vinelandii is a pyridoxal phosphate-containing homodimer that catalyzes

99 e enzyme binds the substrate cysteine in the pyridoxal phosphate-containing site, and a persulfide is

100 rnal aldimine form of NtdA with the cofactor pyridoxal phosphate covalently attached to Lys-247.

101 The pyridoxal phosphate dependent alanine racemase catalyzes

102 ucose-6-phosphate 3-dehydrogenase, NtdA is a pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:

103 ylacetaldehyde (4-HPAA), Rhodiola contains a pyridoxal phosphate-dependent 4-HPAA synthase that direc

104 at the primary amino acid level with several pyridoxal phosphate-dependent amino acid decarboxylases.

105 The S-signal was generated by the pyridoxal phosphate-dependent aminotransferase ScrA; sig

106 ine beta-synthase found in yeast catalyzes a pyridoxal phosphate-dependent condensation of homocystei

107 Sequence homology identified the pyridoxal phosphate-dependent decarboxylase-like protein

108 ts that it may be a member of a new class of pyridoxal phosphate-dependent decarboxylases.

109 nzymological source of the amine moiety as a pyridoxal phosphate-dependent decarboxylating enzyme tha

110 uted cysteine desulfurase that catalyzes the pyridoxal phosphate-dependent desulfuration of L-cystein

111 The pyridoxal phosphate-dependent enzyme 1-aminocyclopropane

112 The catalytic effects of perdeuterating the pyridoxal phosphate-dependent enzyme alanine racemase fr

113 S. cerevisiae FKF had been reported to be a pyridoxal phosphate-dependent enzyme encoded by BNA3.

114 Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a ga

115 oxymethyltransferase (SHMT) is a tetrameric, pyridoxal phosphate-dependent enzyme that catalyzes the

116 Serine hydroxymethyltransferase (SHMT) is a pyridoxal phosphate-dependent enzyme that catalyzes the

117 Cystathionine beta-synthase (CBS) is a pyridoxal phosphate-dependent enzyme that catalyzes the

118 e aminotransferase (GABA-AT) is a tetrameric pyridoxal phosphate-dependent enzyme that catalyzes tran

119 The pyridoxal phosphate-dependent enzyme, 1-aminocyclopropan

120 te, which is converted to Ala(P) by a second pyridoxal phosphate-dependent enzyme, DhpD.

121 site of cystathionine beta-synthase (CBS), a pyridoxal phosphate-dependent enzyme.

122 rboxylate (ACC) synthase as well as of other pyridoxal phosphate-dependent enzymes.

123 a sugar aminotransferase that catalyzes the pyridoxal phosphate-dependent equatorial transamination

124 Alanine racemase catalyzes the pyridoxal phosphate-dependent interconversion of the D-

125 vivo and in vitro enzyme assays, supports a pyridoxal phosphate-dependent mechanism of Sec-tRNA(Sec)

126 y Sep-tRNA:Cys-tRNA synthase (SepCysS) via a pyridoxal phosphate-dependent mechanism.

127 erature, and of isotopic substitution on the pyridoxal phosphate-dependent reaction of L-serine with

128 and homocysteine to form cystathionine in a pyridoxal phosphate-dependent reaction.

129 steine, a toxic metabolite, with serine in a pyridoxal phosphate-dependent reaction.

130 Monovalent cations activate the pyridoxal phosphate-dependent reactions of tryptophan sy

131 Among them, we identified the L136 gene as a pyridoxal phosphate-dependent sugar aminotransferase.

132 e and that AGXT2L1 and AGXT2L2 catalyzed the pyridoxal-phosphate-dependent breakdown of phosphoethano

133 Cystathionine beta-synthase (CBS) is a pyridoxal-phosphate-dependent enzyme that catalyzes a be

134 three-gene operon that potentially encodes a pyridoxal-phosphate-dependent enzyme, an extracellular s

135 1 and AGXT2L2, two closely related, putative pyridoxal-phosphate-dependent enzymes encoded by vertebr

136 not necessarily required for recognition of pyridoxal phosphate derivatives.

137 homogeneous enzyme were characteristic of a pyridoxal phosphate enzyme and showed the absence of hem

138 (EC 2.1.2.1), a member of the alpha-class of pyridoxal phosphate enzymes, catalyzes the reversible in

139 which belong to two different fold types of pyridoxal phosphate enzymes: an aspartate aminotransfera

140 anistic studies showed that the enzyme-bound pyridoxal phosphate forms a Schiff's base with the amino

141 cular terms together with the earlier solved pyridoxal phosphate forms of the enzyme.

142 plants, the pathway for de novo synthesis of pyridoxal phosphate has been well characterized, however

143 In contrast, pyridoxal phosphate (IC50 = 18 microM and coumermycin A1

144 PRK-K53M retains sensitivity to pyridoxal phosphate, implicating K165 as the target of t

145 nd in stabilizing the correct orientation of pyridoxal phosphate in the active site of the beta subun

146 t the active site lysine that normally binds pyridoxal phosphate in the cSHMT protein is exposed to s

147 nd to interact with the pyridine nitrogen of pyridoxal phosphate in the structure of alanine racemase

148 imelate epimerase is a representative of the pyridoxal phosphate-independent amino acid racemases, fo

149 To study the physiological role of Ala AT, a pyridoxal phosphate inhibitor, aminooxyacetic acid, was

150 to form L-cystathionine through a series of pyridoxal phosphate intermediates.

151 The requirement for pyridoxal phosphate is expected on the basis of the natu

152 Pyridoxal phosphate is the cofactor for the enzyme histi

153 Vitamin B6 (pyridoxal phosphate) is an essential cofactor in enzymat

154 y X-rays to create a covalent linkage of the pyridoxal-phosphate moiety to lysine 120 in the binding

155 se is a unique heme protein that catalyzes a pyridoxal phosphate (or PLP)-dependent beta-replacement

156 ositions Thr313 for its interaction with the pyridoxal phosphate oxygens and substrate alpha-carboxyl

157 ciated with plasma folate, vitamin B-12, and pyridoxal phosphate (P for trend < 0.001).

158 ervation that the active form of vitamin B6 (pyridoxal phosphate, P5P) modulates the self-assembly of

159 e one, are dimers containing one molecule of pyridoxal phosphate per subunit.

160 e vitamin B-6 vitamer composition of plasma [pyridoxal phosphate (PLP) > pyridoxic acid (PA) > pyrido

161 olated and recombinant enzymes are devoid of pyridoxal phosphate (PLP) and are rapidly inactivated up

162 It is unique in being dependent on both pyridoxal phosphate (PLP) and heme for activity.

163 Plasma pyridoxal phosphate (PLP) and pyridoxal concentrations a

164 SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolygluta

165 transaminase is a bacterial enzyme that uses pyridoxal phosphate (PLP) as a cofactor to catalyze the

166 Nonenzymatic pyridoxal phosphate (PLP) catalyzed decarboxylations and

167 Heme and pyridoxal phosphate (PLP) cofactors are necessary to cat

168 The pyridoxal phosphate (PLP) dependent enzyme dialkylglycin

169 D-Amino acid transaminase, a pyridoxal phosphate (PLP) enzyme, is inactivated by its

170 I reflect on my research on pyridoxal phosphate (PLP) enzymes over fifty-five years

171 ve been determined crystallographically: the pyridoxal phosphate (PLP) form and a complex with the re

172 ridoxal 5'-phosphate and failure to maintain pyridoxal phosphate (PLP) levels results in epilepsy.

173 enzyme that requires the cofactors heme and pyridoxal phosphate (PLP) to catalyze the condensation o

174 their cofactors (the pterins and vitamin B6 (pyridoxal phosphate (PLP))) in human cerebrospinal fluid

175 Baseline serum riboflavin, pyridoxal phosphate (PLP), folate, vitamin B12, and flav

176 CAS and the Gm-CAS K95A mutant with a linked pyridoxal phosphate (PLP)-Cys molecule in the active sit

177 a-synthase is a hemeprotein that catalyzes a pyridoxal phosphate (PLP)-dependent condensation of seri

178 Both classes of enzymes catalyze a pyridoxal phosphate (PLP)-dependent condensation of seri

179 nzyme is a unique hemeprotein that catalyzes pyridoxal phosphate (PLP)-dependent condensation of seri

180 Cysteine desulfurases perform pyridoxal phosphate (PLP)-dependent desulfuration of cys

181 t: (i) the bioinformatics analysis reveals a pyridoxal phosphate (PLP)-dependent domain, we termed cy

182 n to be a mechanism-based inactivator of the pyridoxal phosphate (PLP)-dependent enzyme gamma-aminobu

183 xymethyltransferase (cSHMT) is a tetrameric, pyridoxal phosphate (PLP)-dependent enzyme that catalyze

184 lglycine decarboxylase (DGD) is a tetrameric pyridoxal phosphate (PLP)-dependent enzyme that catalyze

185 to the recent report that E. coli BioB is a pyridoxal phosphate (PLP)-dependent enzyme with intrinsi

186 Pyridoxal phosphate (PLP)-dependent enzymes are unrivale

187 Pyridoxal phosphate (PLP)-dependent enzymes catalyze man

188 The mechanistic fate of pyridoxal phosphate (PLP)-dependent enzymes diverges aft

189 l therapies for inherited diseases involving pyridoxal phosphate (PLP)-dependent enzymes, including p

190 ase (PanD), the enzyme in M. jannaschii is a pyridoxal phosphate (PLP)-dependent l-aspartate decarbox

191 onic acid (DAPA) synthase (EC 2.6.1.62) is a pyridoxal phosphate (PLP)-dependent transaminase that ca

192 inactivated by the lysine-specific reagent, pyridoxal phosphate (PLP).

193 amin pyrimidine is formed from histidine and pyridoxal phosphate (PLP).

194 ecies (ROS) detoxification and production of pyridoxal phosphate (PLP).

195 l of homocysteine in mammals begins with the pyridoxal phosphate- (PLP-) dependent beta-replacement r

196 The active sites of the homologous pyridoxal phosphate- (PLP-) dependent enzymes 1-aminocyc

197 S-adenosyl-L-methionine (SAM) in the modeled pyridoxal phosphate quinonoid complex with SAM.

198 hildren with intractable seizures respond to pyridoxal phosphate rather than pyridoxine, including a

199 bilizing the protonated pyridine nitrogen of pyridoxal phosphate, reducing the pKa of the internal al

200 and formation of the external aldimine with pyridoxal phosphate required for early steps in SufS cat

201 optimum, Km values, and the requirement for pyridoxal phosphate resemble those of bacterial racemase

202 r pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway.

203 two enzymes of the biosynthetic pathway for pyridoxal phosphate (SerC and PdxA), we have found that

204 kinase (PRK) is inactivated upon exposure to pyridoxal phosphate/sodium borohydride, suggesting a rea

205 ues 1-353) is catalytically active and binds pyridoxal phosphate stoichiometrically establishes that

206 concentrations of glutamate or the cofactor pyridoxal phosphate, suggesting a noncompetitive inhibit

207 te kinetic parameters including the K(d) for pyridoxal phosphate, suggesting that the C-terminal resi

208 lyzed by this enzyme suggests a role for the pyridoxal phosphate, the role of the heme is uncertain.

209 lly thought to be due to abnormal binding of pyridoxal phosphate to glutamic acid decarboxylase resul

210 referred to as Pat) that, in the presence of pyridoxal phosphate, transfers the primary amino group o

211 We hypothesized that pyridoxal phosphate (vitamin B6 coenzyme) was de-activat

212 The pyridoxal phosphate was converted to the pyridoxamine fo

213 Fasting plasma concentration of pyridoxal phosphate was inversely associated with myocar

214 5'-phosphate as the product, indicated that pyridoxal phosphate was not directly involved in the rea

215 Plasma levels of pyridoxal phosphate were inversely associated with risk

216 Pyridoxal phosphate, which binds at the intersubunit act