ライフサイエンスコーパス: pyrin

1 ich encodes a 781-amino acid protein denoted pyrin.

2 were cleaved more efficiently than wild-type pyrin.

3 evere familial Mediterranean fever in TRIM20/pyrin.

4 rocessing and release parallels the level of pyrin.

5 familial Mediterranean fever disease protein Pyrin.

6 es PKN1 and PKN2 that bind and phosphorylate pyrin.

7 tment domain, and the innate immune receptor pyrin.

8 Pyrin, a protein of 781 aa with pyrin domain at the N-te

9 um difficile and its enterotoxin A (TcdA) as Pyrin-activating agents and show that wild-type and FMF

10 urther demonstrate that microtubules control Pyrin activation downstream of Pyrin dephosphorylation a

11 e more effective than WT PSTPIP1 in inducing pyrin activation.

12 results are consistent with a model in which pyrin acts to limit the release of IL-1beta generated by

13 omain modulates pUL83-mediated inhibition of pyrin aggregation.

14 The mechanism by which mutations in pyrin alter protein function to cause episodic inflammat

15 r small interfering RNA against pyrin or YFP-pyrin and ASC (YFP-ASC) were infected with B. cenocepaci

16 y proteins, POP2 is less like the prototypic pyrin and ASC PYDs.

17 Coimmunoprecipitations of pyrin and caspase-1 from THP-1 human monocytic cells wer

18 tions of Mediterranean fever (MEFV) encoding pyrin and characterized by inflammatory attacks induced

19 es have been proposed, including the role of pyrin and cryopyrin in regulating inflammation.

20 rich repeat-containing receptors (NLRs), and pyrin and HIN domain (PYHIN) families.

21 and human cells, here we identify the PYHIN (pyrin and HIN domain-containing protein) family member a

22 An intramolecular complex of the AIM2 Pyrin and HIN domains in an autoinhibited state is liber

23 repeat-containing) superfamily or the PYHIN (PYRIN and HIN-200 domain-containing) superfamily.

24 Absolute and relative quantities of cleaved pyrin and IkappaB-alpha degradation products were substa

25 Mutations in the genes encoding pyrin and mevalonate kinase (MVK) cause distinct interle

26 REDD1 in autolysosomes colocalizes with pyrin and nucleotide-binding domain, leucine-rich repeat

27 Knockdown of pyrin and selective inhibition of p38 MAPK greatly atten

28 We transfected HEK293T cells with pyrin and wild-type and mutated WDR1 Mutant protein form

29 Proteins with pyrin and/or caspase recruitment domains have roles in a

30 lpha and disease-associated proteins TRIM20 (pyrin) and TRIM21.

31 Mutations in the gene encoding pyrin are associated with autoinflammatory disorder Fami

32 ating agents and show that wild-type and FMF Pyrin are differentially controlled by microtubules.

33 ons in the Mediterranean fever gene (MEFV or pyrin) are associated with hereditary autoinflammatory d

34 Molecular docking of pyrin-ASC PYD complexes showed that pyrin PYD can simult

35 onuclear cells infected with B. cenocepacia, pyrin associates with caspase-1 and ASC forming an infla

36 which an evolutionarily conserved N-terminal pyrin association domain (PAD) binds IFI16.

37 nduced activation of mononuclear phagocytes, pyrin augments IL-1beta processing and release.

38 eraction with ASC, it also bound less to the pyrin B-box domain responsible for autoinhibition and he

39 Phosphorylated pyrin bound to 14-3-3 proteins, regulatory proteins that

40 sed by mutations in the inflammasome adaptor Pyrin, but how FMF mutations alter signaling in FMF pati

41 ore, constitutive ligation and activation of pyrin by mutant PSTPIP1 proteins explain the autoinflamm

42 kinases PRK1 and PRK2 to negatively regulate pyrin by phosphorylation.

43 TPIP1, which is also a homotrimer, activates pyrin by unmasking its PYD, thereby allowing it to inter

44 Wild-type human pyrin can also form an inflammasome complex with ASC aft

45 Expression of pyrin can be induced by LPS and cytokines, and IL-10 is

46 Furthermore, fibers of recombinant PYRIN can convert ASC into functional polymers capable o

47 HEK293 cells were transfected with pyrin, caspase-1, apoptotic speck protein with a caspase

48 ion was dependent on inflammasome components pyrin-caspase recruitment domain/apoptotic speck-contain

49 Our data support a new pyrin/caspase-1 pathway for NF-kappaB activation.

50 mutations in the C-terminal B30.2 domain of pyrin cause familial Mediterranean fever (FMF), the most

51 ASC PYD can self-associate and interact with pyrin, consistent with previous reports that pyrin promo

52 otide-binding domain and leucine rich repeat pyrin containing 1b (NLRP1b) inflammasome was identified

53 e-binding domain, leucine-rich family (NLR), pyrin-containing 3 (NLRP3) inflammasome has received muc

54 We generated both pyrin-deficient mice and "knockin" mice harboring mutant

55 Homozygous knockin, but not pyrin-deficient, mice exhibited spontaneous bone marrow-

56 bules control Pyrin activation downstream of Pyrin dephosphorylation and that FMF mutations enable mi

57 200 domain of AIM2 binds to DNA, whereas the pyrin domain (but not that of the other PYHIN family mem

58 It is composed of an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment

59 omain structure, consisting of an N-terminal pyrin domain (PYD) and a C-terminal caspase-recruitment

60 e to intramolecular interactions between its pyrin domain (PYD) and B-box.

61 hich consists of two domains, the N-terminal pyrin domain (PYD) and the C-terminal CARD.

62 izing double-stranded DNA and its N-terminal pyrin domain (PYD) for eliciting downstream effects thro

63 ary inflammasome complexes, achieved through pyrin domain (PYD) interactions between sensors and ASC

64 otein ASC and initiate polymerization of its pyrin domain (PYD) into filaments.

65 on dsDNA engagement, the AIM2 amino-terminal pyrin domain (PYD) is responsible for downstream signali

66 rk of highly intercrossed filaments, whereas pyrin domain (PYD) or caspase activation and recruitment

67 Pyrin domain (PYD) proteins have recently emerged as imp

68 Many pyrin domain (PYD) proteins modulate NF-kappaB activity

69 recruitment domain (CARD) subfamily, and the pyrin domain (PYD) subfamily is one of the largest domai

70 ng studies on human growth hormone (hGH) and pyrin domain (PYD), and the results show how mutations a

71 uman NLRP1, mouse NLRP1b lacks an N-terminal pyrin domain (PYD), indicating that the assembly of the

72 Inflammasome assembly requires the PYRIN domain (PYD)-containing adaptor ASC, and depends o

73 Here we identified the PYRIN domain (PYD)-only protein POP3, which competes wit

74 tion is controlled by phosphorylation of its pyrin domain (PYD).

75 nod-like receptor family member containing a pyrin domain 3 (NLRP3) inflammasome in a protracted mann

76 diated by the NOD-like receptor containing a pyrin domain 3 (NLRP3) inflammasome, although exactly ho

77 and leucine-rich repeat receptor containing pyrin domain 3 (NLRP3) inflammasome.

78 gomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasomes and induces the rel

79 he absence of Nod-like receptor containing a pyrin domain 3 and absent in melanoma 2.

80 elanoma 2 and Nod-like receptor containing a pyrin domain 3 are partially required for caspase-1 acti

81 otide-binding domain and leucine-rich repeat pyrin domain 3 are simultaneously present in the same in

82 amily member that contains an amino-terminal pyrin domain and a carboxy-terminal oligonucleotide/olig

83 ein containing a caspase recruitment domain) pyrin domain and the IFI16-double stranded DNA complex h

84 Pyrin, a protein of 781 aa with pyrin domain at the N-terminal, negatively regulates inf

85 ing dsDNA, only the Aim2 protein through its pyrin domain can form an inflammasome to activate caspas

86 gomerization domain, leucine rich repeat and pyrin domain containing 1 (NLRP1), NLRP3, and nucleotide

87 these sensors, including NLRP1 (NLR family, pyrin domain containing 1), are described to form an inf

88 ng the NOD-like receptor NLRP10 (NLR family, pyrin domain containing 10); however, the mechanism by w

89 merization domain (NOD)-like receptor family pyrin domain containing 12 (NLRP12) plays a protective r

90 One NLR gene, NLRP12 (NLR family, pyrin domain containing 12)/Monarch-1, has emerged as an

91 ucine-rich repeat containing protein family, pyrin domain containing 3 (NLRP3) (cryopyrin or NALP3) a

92 uble deficiency of Nod like receptor family, pyrin domain containing 3 (NLRP3) and caspase 8 inPstpip

93 In this context, NLR family pyrin domain containing 3 (NLRP3) inflammasome activatio

94 n and leucine rich repeat containing family, pyrin domain containing 3 (NLRP3) inflammasome as an ess

95 ding domain, leucine-rich-containing family, pyrin domain containing 3 (NLRP3) inflammasome complex a

96 cell sensor leucine-rich-containing family, pyrin domain containing 3 (NLRP3) inflammasome controls

97 NLR family pyrin domain containing 3 (NLRP3) inflammasome has been

98 is a key to induce NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome in macrop

99 itin, activate the NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome in primed

100 Excessive activation of the NLR family pyrin domain containing 3 (NLRP3) inflammasome is involv

101 leucine-rich repeat containing gene family, pyrin domain containing 3 (NLRP3) inflammasome to induce

102 The NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome, a multip

103 oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome-dependent

104 of Tat in priming and activating NLR family pyrin domain containing 3 (NLRP3) inflammasomes in micro

105 ammasome system, mediated by the NLR family, pyrin domain containing 3 (NLRP3) initiating protein, wa

106 leotide-binding domain, leucine-rich repeat, pyrin domain containing 3 (NLRP3) is a key component of

107 NOD-like receptor family, pyrin domain containing 3 (NLRP3) protein is a key compo

108 rm depended on the NOD-like receptor family, pyrin domain containing 3 (NLRP3) sensor and the apoptos

109 components of the NOD-like receptor family, pyrin domain containing 3 (NLRP3), and absent in melanom

110 n and leucine rich repeat containing family, pyrin domain containing 3 (NLRP3).

111 Activation of the NOD-like receptor family, pyrin domain containing 3 (NLRP3)/caspase-1 inflammasome

112 gomerization domain, leucine-rich repeat and pyrin domain containing 3 (NRLP3) inflammasome members (

113 erization domain (NOD)-like receptor family, pyrin domain containing 3 activation in the inflammasome

114 tightly regulating NOD-like receptor family, pyrin domain containing 3 activation.

115 rich repeat-containing-like receptor family, pyrin domain containing 3 and autophagosome-associated m

116 rvention targeting NOD-like receptor family, pyrin domain containing 3 inflammasome activity induces

117 he key role of the NOD-like receptor family, pyrin domain containing 3 inflammasome during acute pneu

118 n regulate hepatic steatosis; the NLR family pyrin domain containing 3 inflammasome is critically inv

119 he activity of the NOD-like receptor family, pyrin domain containing 3 inflammasome when compared wit

120 ypoxia activate the NOD-like receptor family pyrin domain containing 3 inflammasome.

121 IL-1beta-producing NOD-like receptor family pyrin domain containing 3 inflammasome.

122 otide-binding domain and leucine-rich repeat pyrin domain containing 3 inflammasome.

123 innate immune-sensing complex known as "NLR-Pyrin domain containing 3" (NLRP3) inflammasome, also kn

124 omain leucine-rich repeat containing family, pyrin domain containing 3) inflammasome complex, assembl

125 The NLRP3 (NOD-like receptor family, pyrin domain containing 3) inflammasome is a multiprotei

126 flammasomes, and that the NLRP3 (NLR family, pyrin domain containing 3) inflammasome is not involved

127 OD2 target, NLRP3 (NOD-like receptor family, pyrin domain containing 3) is of importance in the patho

128 e-recruitment domain) and NLRP3 (NLR family, pyrin domain containing 3), which are essential for casp

129 spase-1, and NLRP3 (NOD-Like Receptor family Pyrin domain containing 3).

130 eased expression of inflammatory (NLR family pyrin domain containing 3, interleukins 1beta and 6, and

131 rich repeat-containing-like receptor family, pyrin domain containing 3-associated inflammasomes and i

132 e-1/Caspase-4- and NOD-like receptor family, pyrin domain containing 3-dependent inflammatory cell de

133 ce deficient in the NOD-like receptor family pyrin domain containing 6 (NLRP6) inflammasome feature e

134 g oligomerization domain (NOD)-like receptor pyrin domain containing family of gene 12 (Nlrp12) are a

135 salivary levels of nod-like receptor family pyrin domain containing protein (NLRP) 3, apoptosis-asso

136 tide-binding domain, leucine-rich repeat and pyrin domain containing protein (NLRP) family, which for

137 leotide-binding domain, leucine-rich repeat, pyrin domain containing protein 3 (NLRP3) inflammasome.

138 te the NLRP3 (Nacht, leucine-rich repeat and pyrin domain containing protein 3) inflammasome.

139 (ATG5), ATG7 and ATG16L1 but not NLR family, pyrin domain containing-3 (NALP3).We show that NOD2-medi

140 tly demonstrated that the NOD-like receptor, pyrin domain containing-3 (NLRP3) contributes to renal i

141 nding domain leucine-rich repeat containing, Pyrin domain containing-3 and for absent in melanoma 2 i

142 nding domain leucine-rich repeat containing, Pyrin domain containing-3 inflammasome, and caspase-4 ph

143 ting a role for the NOD-like receptor family pyrin domain containing-3 inflammasome.

144 We show here that the pyrin domain of AIM2 (AIM2(PYD)) drives both filament fo

145 VHHASC leaves the Pyrin domain of ASC functional and stabilizes a filament

146 on the recent near-atomic structures of the PYRIN domain of ASC in the protein filament of inflammas

147 weak affinity, and it is the non-DNA-binding pyrin domain of IFI16 that drives the cooperative filame

148 the structure and dynamics of the N-terminal pyrin domain of NLRP12 (NLRP12 PYD) determined using NMR

149 the structure and dynamics of the N-terminal pyrin domain of NLRP7 (NLRP7 PYD) obtained by NMR spectr

150 The surface residues on the pyrin domain that mediate the cooperative DNA binding ar

151 -like protein containing a CARD) through its pyrin domain to activate caspase-1.

152 unexpected auto-inhibitory function for the pyrin domain, and provide the first genetic evidence lin

153 this response by interacting with the IFI16 pyrin domain, blocking its oligomerization upon DNA sens

154 NLRP1 (NLR family, pyrin domain-containing 1) is a contributor to innate im

155 me components, we found that both NLR family pyrin domain-containing 3 (Nlrp3) and apoptosis-associat

156 -1beta dependent on NOD-like receptor family pyrin domain-containing 3 (NLRP3) and ASC due to the sec

157 mutations in NOD-like receptor (NLR) family, pyrin domain-containing 3 (NLRP3) cause neonatal-onset m

158 oligomerization domain-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome and conco

159 main, leucine-rich repeat-containing family, pyrin domain-containing 3 (NLRP3) inflammasome as well a

160 oligomerization domain-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome drives ma

161 The NLR family pyrin domain-containing 3 (NLRP3) inflammasome has been

162 The NOD-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome is a casp

163 omain-like receptor, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) inflammasome is now no

164 g oligomerization domain-like receptor (NLR) pyrin domain-containing 3 (Nlrp3) inflammasome is though

165 leucine-rich repeat containing family (NLR), pyrin domain-containing 3 (NLRP3) inflammasome plays a k

166 oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome.

167 cleotide-binding domain, leucine-rich repeat pyrin domain-containing 3 (NLRP3) inflammasome.

168 In this study, we showed that NLR family pyrin domain-containing 3 (Nlrp3) is required to induce

169 n (ASC) inflammasomes, including NLR family, pyrin domain-containing 3 (NLRP3), but not NLR family, c

170 ts, including NOD-like receptor (NLR) family pyrin domain-containing 3 (NLRP3), NLRP1, NLR family CAR

171 ng and oligomerization, leucine-rich repeat, pyrin domain-containing 3 (NLRP3), simultaneously and di

172 otide-binding oligomerization domain family, pyrin domain-containing 3 inflammasome activation upon H

173 is detected by the NOD-like receptor family, pyrin domain-containing 3 inflammasome and can trigger a

174 oligomerization domain-like receptor family, pyrin domain-containing 3 inflammasome.

175 and activates the NOD-like receptor family, pyrin domain-containing 3 inflammasome.

176 main, leucine-rich-repeat-containing family, pyrin domain-containing 3) inflammasome mediates product

177 cleotide-binding domain, leucine-rich repeat/pyrin domain-containing 3.

178 cruitment domain-containing 4 or NLR family, pyrin domain-containing 6, are required for triggering t

179 rodent NLRP1B (NACHT leucine-rich repeat and pyrin domain-containing protein 1B).

180 erization domain (NOD)-like receptor family, pyrin domain-containing protein 3 (Nlrp3) expression was

181 The NACHT, LRR, and pyrin domain-containing protein 3 (NLRP3) inflammasome i

182 The NOD-like receptor (NLR) family, pyrin domain-containing protein 3 (NLRP3) inflammasome i

183 binding oligomerization domain-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasomes

184 osolic contact, and activation of NLR family pyrin domain-containing protein 3 (NLRP3) inflammasomes

185 inding oligomerization-like receptor family, pyrin domain-containing protein 3) activation, either by

186 flammasome components Nalp3 (NACHT, LRR, and pyrin domain-containing protein 3), ASC (apoptosis-assoc

187 The SREBP-induced NOD-like receptor family pyrin domain-containing protein inflammasome and its ins

188 e activation of the NOD-like receptor family pyrin domain-containing protein inflammasome in macropha

189 The myxoma virus (MYXV)-encoded pyrin domain-containing protein M013 coregulates inflamm

190 t nucleotide-binding leucine-rich repeat and pyrin domain-containing receptor 12 (NLRP12) impedes alt

191 ding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3) inflammasome controls

192 ding domain, leucine-rich-containing family, pyrin domain-containing-3 (Nlrp3, but also known as Nalp

193 ty, de Almeida et al. (2015) report that the PYRIN domain-only protein (POP1) efficiently inhibits in

194 cent developments in elucidating the role of PYRIN domain-only proteins (POPs) and the related CARD-o

195 Pyrin domain-only proteins (POPs) are recently evolved,

196 e nucleotide oligomerization domain receptor pyrin-domain containing protein 3 (NLRP3) by Salmonella

197 ucine-rich-repeat-containing receptor (NLR), pyrin-domain-containing 3 (NLRP3) inflammasome in human

198 or domains, caspase recruitment domains, and pyrin domains (PYD).

199 Inflammasome assembly is mediated by pyrin domains (PYDs) and caspase recruitment domains, wh

200 ns of caspase recruitment domains (CARDs) or PYRIN domains (PYDs).

201 ntain caspase recruitment domains (CARDs) or pyrin domains (PYDs).

202 , which harbor essential N-terminal CARD and PYRIN domains, respectively.

203 Pyrin exists as a homotrimer in an autoinhibited state d

204 In summary, both pyrin expression and IL-1beta processing and release are

205 To determine whether variations in pyrin expression explain the difference between monocyte

206 ines, and IL-10 is a known potent inducer of pyrin expression in macrophages.

207 downregulates IL-10 and therefore decreases pyrin expression to promote inflammasome activation and

208 e/MDM difference in mature IL-1beta release, pyrin expression was knocked down by nucleofecting small

209 lrp3 inflammasome in the lungs, also induced pyrin expression, which in turn suppressed inflammasome

210 LPS-mediated upregulation of IL-10 enhanced pyrin expression, which serves, particularly in later ph

211 Pyrin forms an inflammasome when mutant or in response t

212 Here, we show that CARD and PYRIN function as bona fide prions in yeast and that the

213 n fever (FMF) MEFV mutations lead to gain of pyrin function, resulting in inappropriate IL-1beta rele

214 In this context, because mutations in the pyrin gene (MEFV) cause the inflammatory disorder famili

215 enes revealed a remarkable deficiency in the pyrin gene, MEFV, expression in MDM compared with monocy

216 r macrophages attenuated the upregulation of pyrin in alveolar macrophages and lung endothelial cells

217 We investigated the role of pyrin in intestinal homeostasis in mice.

218 ion domain can functionally replace CARD and PYRIN in mammalian cell signaling.

219 Similarly, the stable expression of pyrin in PMA-stimulated THP-1-derived macrophages induce

220 ally reconstitute signaling of NLRP3 and ASC PYRINs in mammalian cells.

221 Pyrin induced IL-1beta processing and release in a dose-

222 of microtubules by colchicine also inhibited pyrin inflammasome activation by ribotoxic stress.

223 +) (Pro(2+)) influx kinetics during NLRP3 or Pyrin inflammasome activation in murine bone marrow-deri

224 Remarkably, Pyrin inflammasome activation persisted upon microtubule

225 IDS, led to RhoA inactivation and consequent pyrin inflammasome activation.

226 k host kinases to inhibit effector-triggered pyrin inflammasome activation.

227 How the pyrin inflammasome is activated in the absence of diseas

228 te that ribotoxic stress activates the human pyrin inflammasome through a mechanism that requires p38

229 ic stress triggers the assembly of the human pyrin inflammasome, leading to ASC oligomerization and c

230 ation by pathogens was shown to activate the pyrin inflammasome, so our data now extend this guard hy

231 this recognition pathway by restricting the pyrin inflammasome, thus increasing bacterial fitness.

232 in PYDs that mediates ASC recruitment to the pyrin inflammasome, which is implicated in the pathogene

233 we show that YopM specifically restricts the pyrin inflammasome, which is triggered by the RhoA-inact

234 stably reconstituted with components of the pyrin inflammasome.

235 responses and constitutive activation of the Pyrin inflammasome.

236 h and inflammation through activation of the Pyrin inflammasome.

237 regulatory proteins that in turn blocked the pyrin inflammasome.

238 Pro(2+) influx after initiation of NLRP3 or Pyrin inflammasomes by nigericin (NG) or Clostridium dif

239 Screening of genes encoding pyrin-interacting proteins identified rare mutations in

240 nucleofecting small interfering RNA against pyrin into monocytes or stably transducing small interfe

241 ly transducing small interfering RNA against pyrin into the monocyte cell line, THP-1.

242 We provide evidence that pyrin is a cytosolic receptor for PSTPIP1.

243 Pyrin is an innate immune sensor that forms an active in

244 atory disorder familial Mediterranean fever, pyrin is believed to regulate IL-1beta processing.

245 elf be a caspase-1 substrate, and found that pyrin is cleaved by caspase-1 at Asp330, a site remote f

246 When pyrin is ectopically expressed or silenced, IL-1beta pro

247 In this study, we report that human pyrin is required to detect intracellular B. cenocepacia

248 regulator of neutrophil function upstream to pyrin, is involved in NET release and regulation of IL-1

249 Pyrin knockdown was associated with a significant drop i

250 uation of a yopM mutant is fully reversed in pyrin knockout mice, demonstrating that YopM inhibits py

251 Importantly, M-CSF treatment of MDM restored pyrin levels and IL-1beta release.

252 not demonstrate a significant difference in pyrin levels between patients with a single mutation and

253 Pyrin levels directly correlated with IL-1beta processin

254 Suppression of pyrin levels in monocytes and THP-1 cells reduced caspas

255 In summary, intracellular pyrin levels positively regulate MDM IL-1beta responsive

256 To determine whether pyrin levels were responsible for the monocyte/MDM diffe

257 se microtubule assembly inhibitors prevented Pyrin-mediated caspase-1 activation and secretion of IL-

258 dies with DSS-induced colitis, we found that pyrin (MEFV) is required for inflammasome activation and

259 We now asked whether pyrin might itself be a caspase-1 substrate, and found t

260 and macrophages; therefore, we asked whether pyrin might promote IL-1beta processing and release.

261 hrough cognate interaction of its N-terminal PYRIN motif with the ASC adaptor protein.

262 Although monocytes express pyrin mRNA and protein, which is readily inducible by en

263 rived macrophages express significantly less pyrin mRNA and protein.

264 ndent inflammasome in which gain-of-function pyrin mutations cause autoinflammatory disease.

265 spase-1 activation in which gain-of-function PYRIN mutations lead to IL-1beta cytokine overproduction

266 The discovery that Pyrin mutations remove the obligatory requirement for mi

267 pase activation and recruitment domain; PYD, pyrin N-terminal homology domain; ATF, activating transc

268 Pyrin-only proteins (POPs) are restricted to Old World m

269 "Pyrin-only" proteins (POP) are attractive as negative re

270 In contrast, overexpression of pyrin or ASC induced a robust IL-1beta response to B. ce

271 ment R-Smad signaling because the N-terminal Pyrin or C-terminal leucine-rich repeat domains were dis

272 eir N-terminal effector domains (typically a pyrin or caspase activation and recruitment domain) are

273 ressing either small interfering RNA against pyrin or YFP-pyrin and ASC (YFP-ASC) were infected with

274 Mutated pyrin prohibits this colocalization, leading to higher I

275 pyrin, consistent with previous reports that pyrin promotes ASC clustering to form a proinflammatory

276 Pyrin protein levels were examined by Western blotting.

277 nock-in mouse strain that expresses chimeric pyrin protein with a V726A mutation (Mefv(V726A/V726A))

278 erranean fever is caused by mutations of the PYRIN protein.

279 ots confirmed a corresponding deficit in MDM pyrin protein.

280 eted binding modes, involving three sites on pyrin PYD and two sites on ASC PYD.

281 cking of pyrin-ASC PYD complexes showed that pyrin PYD can simultaneously interact with up to three A

282 , on structural and functional properties of pyrin PYD were investigated.

283 We demonstrate that both the ASC and pyrin PYDs have multifaceted binding modes, involving th

284 have studied the interaction between ASC and pyrin PYDs that mediates ASC recruitment to the pyrin in

285 , Asp(8), and Glu(16), believed critical for Pyrin/Pyrin domain interaction, are important for inflam

286 We have previously shown that pyrin regulates caspase-1 activation and IL-1beta produc

287 Mutations in MEFV (encoding human PYRIN) result in autoinflammatory familial Mediterranean

288 Because of their high binding affinity to pyrin's B-box, PAPA-associated PSTPIP1 mutants were foun

289 Conversely, pyrin small interference RNA suppressed pro-IL-1beta pro

290 P1 is an adaptor protein that interacts with PYRIN, the protein encoded by the Mediterranean Fever (M

291 Pyrin, the protein mutated in FMF, regulates caspase-1 a

292 ormed aggregates that appeared to accumulate pyrin; this could potentially precipitate inflammasome a

293 res the familial Mediterranean fever protein pyrin to assemble the ASC pyroptosome, a molecular platf

294 ckout mice, demonstrating that YopM inhibits pyrin to promote virulence.

295 s of myeloid lineage and encodes the protein pyrin/TRIM20/Marenostrin.

296 Pyrin variants harboring FMF-associated B30.2 mutations

297 rophages in IL-1beta processing and release, pyrin was studied in human monocytes and monocyte-derive

298 -3 and PKN proteins to FMF-associated mutant pyrin was substantially decreased, and the constitutive

299 Human pyrin with gain-of-function mutations in its B30.2/SPRY

300 The interaction of N-terminal pyrin with IkappaB-alpha induced calpain-mediated degrad