ライフサイエンスコーパス: pyrosequencing

1 re collected and profiled with 16S rRNA gene pyrosequencing.

2 fic DNA methylation analysis in plants using pyrosequencing.

3 rongosa National Park, Mozambique) using 454 pyrosequencing.

4 olution using high-throughput locus-specific pyrosequencing.

5 ection or a limitation of the sensitivity of pyrosequencing.

6 Findings were validated using bisulfite pyrosequencing.

7 The microbiota was monitored using 454 pyrosequencing.

8 The methylation status was confirmed by pyrosequencing.

9 ks and 6 months by 16S-based GS-FLX-titanium-pyrosequencing.

10 as performed on cellular RNA using Roche/454 pyrosequencing.

11 ory protocol) and by bacterial 16S rRNA gene pyrosequencing.

12 ma RNA and cell DNA specimens by OLA and 454 pyrosequencing.

13 d and analyzed by 16S ribosomal RNA targeted pyrosequencing.

14 A, and one at 69% by OLA and not detected by pyrosequencing.

15 es in the leptin promoter was measured using pyrosequencing.

16 ed annually between 5 and 14 years of age by pyrosequencing.

17 (n = 73) and at age of 4.5 years (n = 61) by pyrosequencing.

18 Clostridiaceae) inferred from 16S rRNA gene pyrosequencing.

19 nd 24 months were characterized by 16S-based pyrosequencing.

20 ta, across this landscape using qPCR and 454 pyrosequencing.

21 ota composition (dysbiosis) was evaluated by Pyrosequencing.

22 o those that were previously described using pyrosequencing.

23 ate landscape using 16S rRNA gene tagged 454 pyrosequencing.

24 A quantitative polymerase chain reaction and pyrosequencing.

25 mpared with standard single-region Roche 454 Pyrosequencing.

26 g enzymes and transporters were evaluated by pyrosequencing.

27 mor tissues and 9 matching normal tissues by pyrosequencing.

28 idization (FISH), and 16S rRNA gene amplicon pyrosequencing.

29 rom human liver (n = 22) was performed using pyrosequencing.

30 ced with Roche GS-FLX (Genome Sequencer-FLX) pyrosequencing.

31 analyzed by polymerase chain reaction (PCR)-pyrosequencing.

32 acterial populations were analyzed using 454 pyrosequencing.

33 y and environmentally relevant species using pyrosequencing.

34 nalyzed using anaerobic culture and 16S rDNA pyrosequencing.

35 children was determined by 16S ribosomal RNA pyrosequencing.

36 the real-time triplex PCR were confirmed by pyrosequencing.

37 pG sites within the SFRP1 promoter region by pyrosequencing.

38 arate validation cohort of 30 MF patients by pyrosequencing.

39 C4 in response to exposure were validated by pyrosequencing.

40 vered Antarctic lakes by 16S rRNA gene-based pyrosequencing.

41 ity was tracked using 16S rRNA gene amplicon pyrosequencing.

42 munity data from two distinct studies by 454 pyrosequencing.

43 a viridis and Actinia equina using 16 S rDNA pyrosequencing.

44 n additional tumor samples using RT-qPCR and pyrosequencing.

45 eir promoter regions was analyzed by PCR and pyrosequencing.

46 r working with the raw flowgrams obtained by pyrosequencing.

47 16S rRNA gene polymerase chain reaction and pyrosequencing.

48 (96/126) with follow-up target-specific PCR/pyrosequencing.

49 first examination were analyzed by ultradeep pyrosequencing.

50 All controls screened negative by PCR/pyrosequencing.

51 was compared to quantitative PCR (qPCR) and pyrosequencing.

52 analyzed by 16S ribosomal RNA gene amplicon pyrosequencing.

53 hwestern Mediterranean Sea surface waters by pyrosequencing 16S rDNA and rRNA.

54 Candidate genes were validated by bisulfite pyrosequencing (30 AGA, 21 SGA) and also analyzed in cor

55 For 42 real pyrosequencing ('454') datasets, assembly increased the

56 ); among 60 (61%) A (H3N2) viruses tested by pyrosequencing, 53 (88%) belonged to the drifted 3C.2a g

57 ssay results with phenotypic DST (97.0%) and pyrosequencing (97.8%) results from the same clinical sa

58 luated its occurrence in clinical samples by pyrosequencing all presumptive isolates of C. albicans s

59 Third, pyrosequencing allowed the detection of OTUs that were e

60 Our 16S rRNA gene pyrosequencing analyses revealed that the soil type dete

61 Based on pyrosequencing analyses, beta-Proteobacteria dominated i

62 Pyrosequencing analysis and qPCR revealed a dramatic tra

63 Pyrosequencing analysis of CpG rich regions, and CpG din

64 16S rDNA metagenomic pyrosequencing analysis of the microbial communities ass

65 Pyrosequencing analysis revealed that Geobacter species

66 Pyrosequencing analysis was conducted to determine the s

67 Using highly quantitative pyrosequencing analysis, we found extensive and highly v

68 dividuals for miRNA expression profiling and pyrosequencing analysis.

69 mucosal microbiota assessed by 16S rRNA gene pyrosequencing and (1)H NMR spectroscopy.

70 The application of pyrosequencing and allele-specific PCR techniques establ

71 ormed on stool samples via 16S ribosomal RNA pyrosequencing and correlations between disease severity

72 Concordance between PCR/pyrosequencing and culture was 96.9% (1,085/1,120) for U

73 (n = 15) were analyzed by 16S ribosomal RNA pyrosequencing and culture-based methods.

74 Bisulphite pyrosequencing and demethylation treatment showed that N

75 umole photons s(-1) m(-2) ) and combined 454-pyrosequencing and enzymatic activity assays to evaluate

76 ological settings by 16S rRNA gene-based 454 pyrosequencing and explored their relationships to filtr

77 Pyrosequencing and fluorescence in situ hybridization an

78 particle purification, genome amplification, pyrosequencing and gene/genome reconstruction and annota

79 me were investigated using 16S rRNA amplicon pyrosequencing and high-throughput quantitative PCR.

80 16S ribosomal RNA gene amplicon pyrosequencing and HPV DNA testing were conducted annual

81 Here, we used 454 pyrosequencing and identity/divergence grid sampling to

82 firmed sepsis samples with Universal 16S PCR/pyrosequencing and in 76.4% (96/126) with follow-up targ

83 ycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism.

84 Here, we used 16S rRNA gene pyrosequencing and metagenomic sequencing to examine ora

85 Quantification by Pyrosequencing and MLPA demonstrated levels of mutant ce

86 trations of approximately 2%, one at 8.8% by pyrosequencing and not detected by OLA, and one at 69% b

87 levels of resistant variants not detected by pyrosequencing and possibly below the threshold for phen

88 Pyrosequencing and qPCR revealed that laundry water micr

89 hberg) and verified hits using bisulfite PCR pyrosequencing and qPCR.

90 ces samples were analyzed by high-throughput pyrosequencing and quantitative real-time polymerase cha

91 thylation and mRNA levels by using bisulfite pyrosequencing and quantitative RT-PCR in monocytes in v

92 sing over 7 million cDNA sequences from both pyrosequencing and Sanger sequencing, we found that a mu

93 According to 16S rRNA pyrosequencing and shotgun metagenome sequencing analyse

94 We applied 454-pyrosequencing and single-cell RT-PCR of bulk and sorted

95 ymorphisms were typed using a combination of pyrosequencing and TaqMan genotyping assays.

96 mmunity DNA from MW309 was analyzed with 454 pyrosequencing and terminal restriction fragment length

97 1A, and caspase 1 was validated by bisulfite pyrosequencing and the findings were reproduced in the r

98 flocks was performed using Illumina and 454 pyrosequencing and the sequenced genomes compared to the

99 e PCR), DNA methylation status (by bisulfite pyrosequencing), and GAL peptide by RIA of the GAL syste

100 L4, STAT6, FOXP3, and RUNX3) was assessed by pyrosequencing, and differences between strata were anal

101 DMP was successfully validated by bisulfite-pyrosequencing, and identified DMPs were tested in postm

102 n primers, multiple DNA barcode markers, 454-pyrosequencing, and Illumina MiSeq sequencing to the sam

103 r 16S rRNA gene techniques (clone libraries, pyrosequencing, and real-time PCR), we show that polymet

104 pecific polymerase chain reaction, bisulfite pyrosequencing, and restriction enzyme-polymerase chain

105 tractive hybridisation (SSH) followed by 454 pyrosequencing, and RT-qPCR methods.

106 uantitative polymerase chain reaction (PCR), pyrosequencing, and standard culture techniques.

107 ogical studies, we developed and validated a pyrosequencing approach that enables identification of s

108 cts were sequenced using both Sanger and 454 pyrosequencing approaches.

109 ns identified by Universal 16S rRNA gene PCR/pyrosequencing as containing staphylococci, streptococci

110 rkers were validated by a single-methylation pyrosequencing assay in an independent cohort of 143 pat

111 e promoter of ADCYAP1R1 (cg11218385) using a pyrosequencing assay in DNA from white blood cells.

112 A pyrosequencing assay was established that reproduced the

113 A novel pyrosequencing assay was used to determine genetic group

114 ured by bisulphite-polymerase chain reaction pyrosequencing assay.

115 ions were verified by MassARRAY Epityper and pyrosequencing assays and could be further replicated in

116 R amplification on bisulfite-treated DNA and pyrosequencing assays as well as the quantification of t

117 A methylation using bisulfite conversion and pyrosequencing assays on DNA from buccal cells provided

118 distinguish these variants reliably and thus pyrosequencing assays were developed.

119 ee sources are suitable for standard PCR and pyrosequencing assays.

120 cortex and performed quantitative bisulfite pyrosequencing at nine loci.

121 Follow-up bisulfite pyrosequencing at promoter regions showed an increase in

122 as largely due to bacteria being detected by pyrosequencing but not by culture.

123 s readily adopted by another laboratory with pyrosequencing capabilities.

124 riteria for pyrosequence processing or among pyrosequencing, cloning and microscopy, while taxon iden

125 ely 7.5 h when including target-specific PCR/pyrosequencing compared to 27.9 +/- 13.6 h for Gram stai

126 veloped in-house), were utilized to expedite pyrosequencing data analysis.

127 We apply the method to 454 amplicon pyrosequencing data obtained from a malaria virulence ge

128 Pyrosequencing data revealed that the dominant phyla rel

129 Analysis of ultradeep pyrosequencing data sets derived from virus amplicons fr

130 The pyrosequencing data were cross-checked by using the sing

131 Targeted bisulfite pyrosequencing demonstrated that miR-34A was inactivated

132 Evaluation of pyrosequencing-derived 16S rRNA gene sequences recovered

133 Pyrosequencing errors, consisting mainly of nucleotide i

134 f 16S rRNA-encoding gene sequence reads (454-pyrosequencing) examined viable and total biofilm commun

135 Pyrosequencing experiments validated the cell type-speci

136 e assessed HVR1 evolution by using ultradeep pyrosequencing for a cohort of treatment-naive, chronica

137 polymerase chain reaction amplification with pyrosequencing for a rapid approach to generate the comp

138 length p17 and p24 gag were generated by 454 pyrosequencing for all pairs near the time of transmissi

139 time point aliquots and analyzed by PCR and pyrosequencing for bacterial rRNA gene targets.

140 We employed bacterial fingerprinting and pyrosequencing for microbiome analysis.

141 Our findings confirm the potential of pyrosequencing for quantifying microbial diversity, but

142 Donor DNA was assayed by pyrosequencing for SP-D polymorphisms of two single-nucl

143 umbricus rubellus through utilizing 16S rRNA pyrosequencing for the first time to establish the micro

144 Independent verification by bisulfite pyrosequencing generally confirmed the percentage methyl

145 Pyrosequencing generated more than 150,000 sequences, re

146 Pyrosequencing genotyping and sequencing of the voltage

147 anscriptome profiling and gene-specific cDNA-pyrosequencing have documented that transcriptome shock

148 all and medium CMNs using Sanger sequencing, pyrosequencing, high-resolution melting analysis, and mu

149 Pyrosequencing identified abundant atypical bacteria in

150 DNA pyrosequencing identified hypermethylated regions of the

151 Thus, pyrosequencing illustrated that while DB, PRB, and SRB r

152 est were verified by means of locus-specific pyrosequencing in 35 sibling pairs and replicated in an

153 thylation was measured by means of bisulfite pyrosequencing in patients.

154 terval [CI]) for Universal 16S rRNA gene PCR/pyrosequencing included sensitivity of 77.8% (69.5 to 84

155 Pyrosequencing indicated biofilm communities were all si

156 lly available high-throughput locus-specific pyrosequencing instruments allow for the simultaneous an

157 motives, which cannot be evaluated by these pyrosequencing instruments due to software limitations.

158 uency by single-genome amplification and 454 pyrosequencing, (iv) virion-associated Env content, and

159 In summary, shotgun pyrosequencing metagenomic analyses of agricultural dust

160 In this study, we performed shotgun pyrosequencing metagenomic analyses of DNA from dusts fr

161 alignment showed that 19% or 62% of shotgun pyrosequencing metagenomic DNA sequence reads from swine

162 Mutational status was determined by pyrosequencing method, allelic discrimination, or Sanger

163 Using a pyrosequencing method, we determined that the tumor posi

164 the Sanger sequencing, the 'next generation' pyrosequencing, microarrays, quantitative PCR, and the r

165 Culture-independent pyrosequencing microbiome analysis was used to examine t

166 However, out of 23 candidates tested by pyrosequencing, none could be confirmed.

167 Repeat pyrosequencing of 13 specimens showed reproducible detec

168 Pyrosequencing of 16S rDNA is widely used to study micro

169 Pyrosequencing of 16S rDNA was used to study changes in

170 Analyses of community composition by pyrosequencing of 16S rRNA and hoxH genes indicate that

171 Fecal and ileal microbiota were analyzed by pyrosequencing of 16S rRNA genes and enumeration of sele

172 In total, 24 samples were examined using pyrosequencing of 16S rRNA genes and various chemical pr

173 Pyrosequencing of 16S rRNA genes results revealed an age

174 Quantitative PCR (qPCR) and pyrosequencing of 16S rRNA genes was used to quantify th

175 ng six marine lakes and one ocean site using pyrosequencing of 16S rRNA genes.

176 Pyrosequencing of 16S rRNA transcripts from the indigeno

177 By combining pyrosequencing of 18S ribosomal RNA genes with data on m

178 acterization was performed by using 16S rDNA pyrosequencing of 872 nasal swabs collected biweekly fro

179 We demonstrate via 16S rRNA gene pyrosequencing of 922 specimens from 58 subjects that th

180 We used 454 pyrosequencing of a normalized adult cDNA library and de

181 shed from C. albicans and C. dubliniensis by pyrosequencing of a short region of ITS2, and we have ev

182 In contrast, SSCP and pyrosequencing of an unrelated single SNP (rs1835740:C >

183 Using pyrosequencing of bacterial 16S rRNA genes, we observed

184 bacterial communities were characterized by pyrosequencing of barcoded 16S rRNA genes and clustered

185 DNA methylation was assessed by pyrosequencing of bisulfite converted DNA and methylated

186 independent sample set of 558 subjects using pyrosequencing of bisulfite-converted DNA (min P-value <

187 ent case-control participants using targeted pyrosequencing of bisulfite-converted DNA.

188 Utilizing extensive transcriptome pyrosequencing of diverse taxa, we established a resolve

189 Direct Sanger sequencing as well as pyrosequencing of environmental isolates demonstrated th

190 Data to support this includes V1-V3 pyrosequencing of formation water and drilling mud, as w

191 and analyzed community composition based on pyrosequencing of fungal internal transcribed spacer (IT

192 Pyrosequencing of genetic marker, COI (cytochrome c oxid

193 geting clades of the xoxF gene, and used 454 pyrosequencing of PCR amplicons obtained from the DNA of

194 DGGE and 454-pyrosequencing of PCR-amplified 16S rRNA genes were used

195 Pyrosequencing of pig caecal 16S rRNA gene amplicons ide

196 MeTIL markers can be determined by bisulfite pyrosequencing of small amounts of DNA from formalin-fix

197 High-throughput pyrosequencing of SSU rDNA genes was used to obtain mont

198 idiopathic IBD (n = 10) were analyzed by 454-pyrosequencing of the 16S rRNA gene and qPCR assays.

199 f 86 school age children was analyzed by 454 pyrosequencing of the 16S rRNA gene fragments.

200 200 paired sputum samples from 93 CF adults; pyrosequencing of the 16S rRNA gene was applied to 59 pa

201 Using 454 pyrosequencing of the 16S rRNA gene, we compared bacteri

202 8) and peri-implantitis (n = 6) sites using pyrosequencing of the 16S rRNA gene.

203 ed soil, rhizosphere, and plant roots by 454-pyrosequencing of the 16S rRNA gene.

204 rm and nonfarm children were analyzed by 454-pyrosequencing of the bacterial 16S ribosomal RNA gene.

205 In this work, pyrosequencing of the bacterial 16S rRNA gene was firstl

206 CM fungal communities were identified by 454 pyrosequencing of the fungal internal transcribed spacer

207 DNA methylation levels were assessed by pyrosequencing of the LINE-1 retroelement promoter in DN

208 es (73.1 and 44.6%), and, as revealed by 454-pyrosequencing of the microbial 16S rRNA gene, decreased

209 Pyrosequencing of the PCR amplicons identified a total o

210 Pyrosequencing of the pfcrt gene codon 76 region was per

211 Pyrosequencing of the two feeding groups found that meth

212 Biofilm samples were analyzed by FLX+ pyrosequencing of the V1 to V4 hypervariable regions of

213 ace sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA

214 amplicons were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform.

215 centage methylation (%5mC) was analysed with pyrosequencing on buccal DNA.

216 tive was to perform whole-genome shotgun 454 pyrosequencing on the same fecal specimens collected in

217 and homozygote mutant genotypes compared to pyrosequencing or Sanger sequencing.

218 In conclusion, the OLA and pyrosequencing performed similarly in the quantification

219 c Gram-negative rods had target-specific PCR/pyrosequencing performed.

220 Pyrosequencing permits accurate quantification of DNA me

221 ger RNA samples were sequenced using the 454 pyrosequencing platform and comparatively analyzed to es

222 e for the presence of similar sequences, the pyrosequencing platform correctly classified all 12 inci

223 le-measure incidence assay by implementing a pyrosequencing platform.

224 critical for ligation of the OLA probes and pyrosequencing primers, respectively.

225 hic locations and then conducted 454 shotgun pyrosequencing procedures to obtain 16-24 x coverage of

226 We analyzed, based on pyrosequencing profiling of the biofilm communities in 1

227 e sample processing was included for PCR and pyrosequencing protocols, the molecular approach yielded

228 We developed a pyrosequencing (PSQ) assay including eight subassays for

229 We described AM fungal communities using 454-pyrosequencing, quantified the proportion of root length

230 own of residence, and experimental plate for pyrosequencing reactions.

231 ified by adenosine triphosphate analysis and pyrosequencing, respectively.

232 The pyrosequencing results corroborated our analysis, withou

233 Pyrosequencing results suggested Dechloromonas and Tetra

234 Based on the pyrosequencing results, it appears that clindamycin has

235 actors most likely to affect the accuracy of pyrosequencing results.

236 The 16S rDNA-targeted pyrosequencing revealed a significant change in the comp

237 Pyrosequencing revealed an increase in percentage of Gra

238 Pyrosequencing revealed pronounced diversity co-exists w

239 Pyrosequencing revealed relatively stable bacterial comm

240 Pyrosequencing revealed shared HIV-1 antibody clonal lin

241 However, pyrosequencing revealed that 15/15 (100%) of patients ha

242 Methylated DNA immunoprecipitation-PCR and pyrosequencing revealed that developmental BPA exposure

243 Pyrosequencing revealed that the population belonging to

244 Community analysis using 16S rRNA pyrosequencing revealed the dominance of Methanosarcina

245 16S amplicon analysis (454 FLX pyrosequencing) revealed significantly lower alpha diver

246 49 subjects with paired specimens tested by pyrosequencing, S. aureus was identified from 11 (22%) a

247 2.0%, and at 125 codons (28%; P < 0.0001) by pyrosequencing sensitive to 0.1%.

248 nvironment unrepresented among 18S rRNA gene pyrosequencing sets.

249 454 pyrosequencing showed a significant selection for diazot

250 16S rRNA amplicon pyrosequencing showed an increase in the relative abunda

251 16S rRNA gene pyrosequencing showed that Gram-positive bacteria affili

252 Pyrosequencing showed that novel strains Peptoniphilacea

253 somal RNA gene libraries sequenced using 454-pyrosequencing targeting the V1 to V3 and V7 to V9 regio

254 Amplicon pyrosequencing targets a known genetic region and thus i

255 Pyrosequencing technology provides an important new appr

256 om DNA analyses of the in situ samples using pyrosequencing technology, we found the highest abundanc

257 gender-matched controls using the bisulphite pyrosequencing technology.

258 ial changes were monitored by amplifying and pyrosequencing the 16 S ribosomal small subunit region.

259 e fecal microbiota from these two species by pyrosequencing the 16S V1-V3 hypervariable regions using

260 We profiled the fecal microbiota by pyrosequencing the gene encoding 16S ribosomal RNA (rRNA

261 We used 16S rRNA gene amplification and pyrosequencing to characterize, for the first time to ou

262 used capillary electrophoresis and Roche 454 pyrosequencing to determine the number of repeats in eac

263 We used next generation 454 pyrosequencing to discern the whole-body microbiome of t

264 s were subjected to whole-genome shotgun 454 pyrosequencing to identify both fecal bacterial populati

265 ions by using phylogenic microarrays and 454 pyrosequencing to identify microorganisms and functional

266 We used pyrosequencing to investigate allelic imbalance of Oxtr

267 tersubtype Env recombinants and utilized 454 pyrosequencing to investigate the distribution of over 4

268 In this work, we employed 454 pyrosequencing to profile the evolution of an oligonucle

269 eotrichia (class Spirotrichea), by comparing pyrosequencing to Sanger-sequenced clone libraries and m

270 Here we compared the results of PCR/pyrosequencing to those of culture for detecting bacteri

271 ten-eleven translocation-assisted bisulfite pyrosequencing, to quantify FMR1 5mC and 5hmC levels.

272 The initial PCR/pyrosequencing took approximately 5.5 h to complete or a

273 ing microscopy, and molecular biology (i.e., pyrosequencing) tools.

274 In this study, we used ultradeep pyrosequencing (UDPS) to map the viral heterogeneity of

275 uencing (NGS), capillary electrophoresis and pyrosequencing under the term 'NGS+' for typing Y-STRs a

276 ive to 2.0%, at 71 codons (16%; P = 0.78) by pyrosequencing using a cutoff value of >/= 2.0%, and at

277 arine sediment cores by quantitative PCR and pyrosequencing using newly designed DEH 16S rRNA gene ta

278 Bisulphite pyrosequencing validated six loci in a further independe

279 Next-generation pyrosequencing validated the congruence of the potential

280 454-pyrosequencing was able to recover 10 more families of a

281 Pyrosequencing was employed to characterize bacteria and

282 ent for detection of bacteria by culture and pyrosequencing was good for aerobic bacteria such as P.

283 While pyrosequencing was more sensitive, detection of mutants

284 Targeted bisulfite pyrosequencing was performed on a validation cohort of 3

285 ity structure appeared to remain stable when pyrosequencing was performed on samples that were not su

286 Pyrosequencing was used for DNA methylation quantificati

287 454 pyrosequencing was used to assign operational taxonomic

288 s prior to DNA extraction, and 16S rRNA gene pyrosequencing was used to characterize their bacterial

289 Bisulfite pyrosequencing was used to compare the methylation level

290 Pyrosequencing was used to confirm the imprinting status

291 Pyrosequencing was used to evaluate deviation from monoa

292 In this study, 454 pyrosequencing was used to identify the bacteria associa

293 Culture-independent high-density Roche 454 pyrosequencing was used to survey the distal gut microbi

294 Next-generation sequencing (454 pyrosequencing) was performed to reveal the CPB transcri

295 Using pyrosequencing we followed up the two most differentiall

296 Using pyrosequencing, we elucidated how important phylotypes o

297 DNA extraction and pyrosequencing were done on several core sections in ord

298 tive polymerase chain reaction (PCR) and 454 pyrosequencing were used to analyze bacterial 16S riboso

299 ferent spot positions, quantitative PCR, and pyrosequencing, were used to confirm the role of the can

300 during monochloramine disinfection using PMA-pyrosequencing, while the community structure appeared t