ライフサイエンスコーパス: pyruvate kinase

1 lysis (phosphoglycerate mutase, enolase, and pyruvate kinase).

2 ness to a heterologous promoter (i.e. l-type pyruvate kinase).

3 n of other ChREBP target genes such as liver pyruvate kinase.

4 synthase, SREBP1c, chREBP, glucokinase, and pyruvate kinase.

5 nonproliferating tumor cells require active pyruvate kinase.

6 tein kinase, while the tetramer is an active pyruvate kinase.

7 the splice isoforms of the glycolytic enzyme pyruvate kinase.

8 ress exclusively the embryonic M2 isoform of pyruvate kinase.

9 its alpha, beta(1), and beta(2) of plastidic pyruvate kinase.

10 of other known cytoplasmic proteins such as pyruvate kinase.

11 lation of the ChREBP target gene, liver-type pyruvate kinase.

12 he expression of the glucose-responsive gene pyruvate kinase.

13 ndicative for phosphorylation to MANT-ATP by pyruvate kinase.

14 the structure (but not sequence homology) to pyruvate kinase.

15 s implicated Mck1p in negative regulation of pyruvate kinase.

16 olism: glucose-6-phosphate dehydrogenase and pyruvate kinase.

17 KM2, the gene encoding the glycolytic enzyme pyruvate kinase.

18 opsis genome encodes 14 putative isoforms of pyruvate kinases.

19 complexes, such as phosporylase B (194 kDa), pyruvate kinase (232 kDa), and GroEL (801 kDa), to highl

20 was considerably enhanced when a myc-tagged pyruvate kinase-6kDa fusion protein was overexpressed in

21 permeability, and reduced concentrations of pyruvate kinase, a biomarker of pediatric gastrointestin

22 anslocation, we found that the M2 isoform of pyruvate kinase, a key enzyme in glycolysis, translocate

23 abnormally low levels of the red cell enzyme pyruvate kinase, a known cause of CNSHA.

24 to regulate target genes, such as liver-type pyruvate kinase, acetyl-CoA carboxylase 1, and fatty aci

25 e curves were classified to rapidly identify pyruvate kinase activators and inhibitors with a variety

26 gulates the conversion of protein kinase and pyruvate kinase activities.

27 a(1) subunit causes a reduction in plastidic pyruvate kinase activity and 60% reduction in seed oil c

28 r cells leads to a decrease in the levels of pyruvate kinase activity and an increase in the pyruvate

29 C-terminal 51 kDa truncation showed not only pyruvate kinase activity but also activation by aspartat

30 Thus, decreased pyruvate kinase activity in PKM2-expressing cells allows

31 kinase regulatory subunit, Bcy1p, increases pyruvate kinase activity in vivo.

32 with the tyrosine phosphopeptide affects the pyruvate kinase activity of PKM2.

33 e of the growth phenotype with pyruvate, and pyruvate kinase activity of purified recombinant PykM.

34 glycolytic enzyme pyruvate kinase M2 (PKAR; pyruvate kinase activity reporter), which multimerizes a

35 its BC cell survival in a dose-dependent but pyruvate kinase activity-independent manner.

36 termediate phosphoenolpyruvate and decreased pyruvate kinase activity.

37 lation by NAD+ concentration at the level of pyruvate kinase activity.

38 hich hinders PKM2 tetramerization and blocks pyruvate kinase activity.

39 ported to have chaperone activity: catalase, pyruvate kinase, albumin, lysozyme, alpha-lactalbumin, a

40 We additionally demonstrated that pyruvate kinase also interacts with Kir6.2 subunits.

41 OK257 cells have high lactate dehydrogenase, pyruvate kinase and 3-hydroxyacyl-CoA dehydrogenase acti

42 ranscription of two divergent genes encoding pyruvate kinase and a putative SOS response nuclease, re

43 Two proteins (pyruvate kinase and albumin) were inferred to be related

44 SFEC, pyruvate kinase and aldolase were co-localized by immuno

45 sphofructokinase, lactate dehydrogenase, and pyruvate kinase and analyzed by confocal microscopy.

46 Surprisingly, pyruvate kinase and catalase were at least as effective

47 tasis, is most likely exerted by the enzymes pyruvate kinase and fructose bisphosphatase.

48 dy, the glucose-mediated induction of L-type pyruvate kinase and glucose-6-phosphatase mRNA levels wa

49 The basal mRNA levels for L-pyruvate kinase and glucose-6-phosphatase were not alter

50 reaction is coupled to NADH oxidation using pyruvate kinase and lactate dehydrogenase.

51 Transcription of the liver type pyruvate kinase and lipogenesis enzyme genes is induced

52 ability because of reduced activity of liver pyruvate kinase and malic enzyme, which replenish pyruva

53 cycle flux, which together with increases of pyruvate kinase and phosphoenolpyruvate carboxylase acti

54 hat growth signals reciprocally regulate the pyruvate kinase and protein kinase activities of PKM2 by

55 udy suggests that the conversion between the pyruvate kinase and protein kinase activities of PKM2 ma

56 lated protein(s) regulates the conversion of pyruvate kinase and protein kinase of PKM2 by directly i

57 ussed in comparisons of malate synthase with pyruvate kinase and pyruvate phosphate dikinase.

58 (FAS) competed with the CHO-RE of the l-type pyruvate kinase and S(14) genes for ChoRF binding.

59 nearly identical to the CHO-RE of the l-type pyruvate kinase and S(14) genes.

60 e factor (Cho- RF), that binds to liver-type pyruvate kinase and S(14) promoters at sites critical fo

61 etrameric (streptavidin, concanavalin A, and pyruvate kinase), and pentameric (C-reactive protein) co

62 zymes involved in central carbon metabolism, pyruvate kinase, and aspartate aminotransferase.

63 Glioma cells expressed both isoforms of pyruvate kinase, and inhibition of either glycolysis or

64 1, Glut-2, Gck, aldo-B, the liver isoform of pyruvate kinase, and insulin expression was reduced in P

65 es that uses succinyl-coenzyme A synthetase, pyruvate kinase, and lactate dehydrogenase to couple the

66 tronic FV vector that expressed EGFP, R-type pyruvate kinase, and MGMTP140K, we were able to increase

67 different genes: fatty-acid synthase, l-type pyruvate kinase, and S(14).

68 igh activities of fructokinase, glucokinase, pyruvate kinase, and tricarboxylic acid cycle enzymes, i

69 ins, including Grp58, Grp78, alpha4-actinin, pyruvate kinase, and vimentin.

70 cluding hexokinase, phosphofructokinase, and pyruvate kinase, appeared to be coordinately down-regula

71 Four isozymes of pyruvate kinase are differentially expressed in human ti

72 coupled with phosphoenol pyruvate (PEP) and pyruvate kinase as an ATP regeneration system.

73 esis required PEP as the phosphate donor and pyruvate kinase as the catalyst.

74 sarcolemmal integrity as determined by serum pyruvate kinase assays.

75 Cytosolic pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC

76 One of these involved inhibition of pyruvate kinase by citrate, which accumulated and thereb

77 o the allosteric inhibition of rabbit muscle pyruvate kinase by phenylalanine.

78 The assay detected 75 nM ADP produced by the pyruvate kinase-catalyzed phosphorylation of pyruvate wi

79 yl transfer and proton transfer steps in the pyruvate kinase-catalyzed reaction are altered; pyruvate

80 Therefore, the identified pyruvate kinase catalyzes a crucial step in the conversi

81 Plastidic pyruvate kinase catalyzes a highly regulated, ATP-produc

82 Pyruvate kinase catalyzes the final step in glycolysis a

83 Pyruvate kinase catalyzes the last and rate-limiting ste

84 The addition of this sequence to pyruvate kinase causes the cytoplasmic protein to be loc

85 ied including, LDH (Ra, Ch), G3PDH (Hu, Ch), pyruvate kinase (Ch), Annexin II (Ch), and protein disul

86 nstructs to the cytoplasmic reporter protein pyruvate kinase confirmed a requirement for both motifs

87 iments with myc-tagged coilin and myc-tagged pyruvate kinase confirmed that coilin is a shuttling pro

88 ose-6-phosphate dehydrogenase deficiency and pyruvate kinase deficiency also confer some degree of re

89 providing a potential cure for patients with pyruvate kinase deficiency, in vivo selection using foam

90 canine model of a severe erythroid disease, pyruvate kinase deficiency.

91 pients with chimerism using a mouse model of pyruvate kinase deficiency.

92 nother to cleave near a mutation that causes pyruvate kinase deficiency.

93 hen transplanted into minimally conditioned, pyruvate kinase-deficient recipients (CBA-Pk-1(slc)/Pk-1

94 levels, reducing the drive for production of pyruvate kinase-deficient red blood cells.

95 olic branch point and of its central enzyme, pyruvate kinase (DeltapykF), result in mutants with sign

96 We identified pykM as the only pyruvate kinase-encoding gene based on deficiency in act

97 Paradoxically, decreased pyruvate kinase enzyme activity accompanies the expressi

98 ite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/s

99 disrupted metabolic activity due to altered pyruvate kinase expression and/or alteration in the func

100 nproliferating tumor cells and no detectable pyruvate kinase expression in proliferating cells.

101 Switching pyruvate kinase expression to the M1 (adult) isoform lea

102 olpyruvate carboxykinase (PEPCK) expression, pyruvate kinase expression was decreased 16-fold in fast

103 erase cleavage while preserving tumor type 2 pyruvate kinase expression.

104 using as a case study the rapid decrease in pyruvate kinase flux in yeast upon glucose removal.

105 The isoform of pyruvate kinase from brain and muscle of mammals (M(1)-P

106 We report X-ray structures of pyruvate kinase from Leishmania mexicana (LmPYK) that ar

107 mparative molecular dynamics analysis of the pyruvate kinase from Leishmania mexicana is presented in

108 The interaction of pyruvate kinase from skeletal (SKPK) and smooth (SMPK) m

109 The catalytic activities of aldolase and pyruvate kinase functionally modulate K(ATP) channels in

110 arbohydrate-responsive element of the l-type pyruvate kinase gene (l-PK).

111 or activation of transcription of the L-type pyruvate kinase gene and lipogenic enzyme genes, and (ii

112 cose response element (CACGTG motifs) of the pyruvate kinase gene and the "insulin response element"

113 (LDHA) expression, together with a switch in pyruvate kinase gene splicing from PKM2 to PKM1, marks t

114 ed in mediating carbohydrate response of the pyruvate kinase gene.

115 he C terminus of a green fluorescent protein-pyruvate kinase (GFP-PK) chimera, the resultant protein

116 sequestrin), glucose metabolism (hexokinase, pyruvate kinase, Glut4), oncogenesis (TGFbeta1, cathepsi

117 hydrogenase, myosin light chain, aldolase A, pyruvate kinase, glycogen phosphorylase, actinin, gamma-

118 glucose transporter-4, hexokinase-2, muscle-pyruvate kinase, hormone-sensitive lipase, and uncouplin

119 phosphoenolpyruvate (PEP), the substrate for pyruvate kinase in cells, can act as a phosphate donor i

120 r, our study identifies an essential role of pyruvate kinase in preventing metabolic block during car

121 .2 protein similarly interact with GAPDH and pyruvate kinase in rat heart membrane fractions and that

122 We report that knockdown of pyruvate kinase in tumor cells leads to a decrease in th

123 e catalytic subunit, phosphorylates purified pyruvate kinase in vitro, and that loss of the cAMP-depe

124 es expression of the embryonic M2 isozyme of pyruvate kinase, in contrast to the M1 isozyme normally

125 APDH, triosephosphate isomerase, and M2-type pyruvate kinase increased approximately two- to threefol

126 is demonstrated by experiments in which the pyruvate kinase inhibitor, phenylalanine, is added to ce

127 Pyruvate kinase is a key glycolytic enzyme.

128 Thus, while yeast pyruvate kinase is covalently modified in response to gl

129 in a splice isoform of the glycolytic enzyme pyruvate kinase is necessary for the shift in cellular m

130 as glycolysis, where pathway outflow through pyruvate kinase is regulated by the concentration of a k

131 Pyruvate kinase is the only regulatory step of the commo

132 d the glycolytic activity of PKM2, the major pyruvate kinase isoenzyme known to regulate cellular glu

133 y a SiLAD proteomics analysis, we identified pyruvate kinase isoenzyme M2 (PKM2), a critical regulato

134 We find that a deficiency in the M2 pyruvate kinase isoform (PKM2) reduces the levels of met

135 , this is partly achieved through control of pyruvate kinase isoform expression.

136 Pyruvate kinase isoform M2 (PKM2) is a glycolysis enzyme

137 Pyruvate kinase isoform M2 (PKM2) is an enzyme-catalyzin

138 It is long known that pyruvate kinase isoform M2 (PKM2) is released into the c

139 Here, we demonstrated that the nuclear pyruvate kinase isoform M2 (PKM2) levels were positively

140 Pyruvate kinase isoform M2 (PKM2) plays an important rol

141 The embryonic pyruvate kinase isoform, PKM2, is almost universally re-

142 The influence of pyruvate kinase isoforms on tumor cells has been extensi

143 The pyruvate kinase isoforms PKM1 and PKM2 are alternatively

144 ed protein product (UNP) similar to enolase, pyruvate kinase, isoforms of creatine kinase, aldolase A

145 The main pyruvate kinase isozyme (Cdc19) is phosphorylated in res

146 ological activators of a tumor cell specific pyruvate kinase isozyme (PKM2) may be an approach for al

147 Human pyruvate kinase isozyme M2 (hPKM2) is expressed in early

148 ng T-complex polypeptide 1 subunit zeta, and pyruvate kinase isozyme.

149 The M1 and M2 pyruvate-kinase isozymes are expressed from a single gen

150 Although pyruvate kinase knockdown results in modest impairment o

151 is from both glucose and glutamine following pyruvate kinase knockdown.

152 ate-responsive element-binding protein-beta, pyruvate kinase L, SCD-1, and DGAT1, key transcriptional

153 onse elements (ChoREs) of the rat liver-type pyruvate kinase (L-PK) and S(14) genes and found them to

154 h glucose and reduced the activity of L-type pyruvate kinase (L-PK) and TxNIP promoters, two well-cha

155 ciprocally regulate expression of the L-type pyruvate kinase (L-PK) gene by controlling the formation

156 Glucose-mediated activation of the L-type pyruvate kinase (L-PK) gene is repressed by cAMP, making

157 glucose-mediated induction of hepatic L-type pyruvate kinase (L-PK) gene transcription.

158 arbohydrate responsive element of the L-type pyruvate kinase (L-PK) gene.

159 tral and basic amino acid transporter, liver pyruvate kinase (L-Pk), and insulin in Hnf-1alpha(-/-) m

160 The initial 26 amino acids of human liver pyruvate kinase (L-PYK) are not present/observed in the

161 ize the regulatory properties of human liver pyruvate kinase (L-PYK), we have noted that the affinity

162 y metabolomics revealed that inactivation of pyruvate kinase leads to accumulation of phosphoenolpyru

163 We quantified gene copy numbers of the pyruvate kinase, liver, and red blood cell (PKLR) gene a

164 by its ability to bind the ChRE of the liver pyruvate kinase (LPK) gene.

165 ent (ChRE) within the promoter of the L-type pyruvate kinase (LPK) gene.

166 three hepatic genes, fatty acid synthase, L-pyruvate kinase (LPK), and the S14 protein (S14).

167 agenesis was used to change Lys 240 of yeast pyruvate kinase (Lys 269 in muscle PK) to Met.

168 splicing in VAChT-deficient mice, including pyruvate kinase M, a key enzyme involved in lactate meta

169 with allosteric regulation in rabbit muscle pyruvate kinase (M(1)-PYK).

170 Here, we assessed whether disrupting pyruvate kinase-M (Pkm), an enzyme that acts in the term

171 Although normal cells express the pyruvate kinase M1 isoform (PKM1), tumor cells predomina

172 human cancer cell lines and replace it with pyruvate kinase M1.

173 ET biosensors based on the glycolytic enzyme pyruvate kinase M2 (PKAR; pyruvate kinase activity repor

174 romote dimerization of the glycolytic enzyme pyruvate kinase M2 (PKM2) and enable its nuclear translo

175 Here we identify pyruvate kinase M2 (PKM2) as a novel PTP1B substrate in

176 In particular, pyruvate kinase M2 (PKM2) expression and activity were u

177 f CLL cells, indicated by down-regulation of pyruvate kinase M2 (PKM2) expression and activity, decre

178 The role of pyruvate kinase M2 (PKM2) in cell proliferation is contr

179 The former, in turn, was mediated by pyruvate kinase M2 (PKM2) interaction with soluble adeny

180 Pyruvate kinase M2 (PKM2) is a key enzyme for glycolysis

181 Pyruvate kinase M2 (PKM2) is a metabolic enzyme that pla

182 Pyruvate kinase M2 (PKM2) is also induced and interacts

183 Tumor-specific pyruvate kinase M2 (PKM2) is essential for the Warburg e

184 Pyruvate kinase M2 (PKM2) is expressed at high levels du

185 Tumor-specific pyruvate kinase M2 (PKM2) is instrumental in both aerobi

186 The embryonic pyruvate kinase M2 (PKM2) isoform is highly expressed in

187 S caused inhibition of the glycolytic enzyme pyruvate kinase M2 (PKM2) through oxidation of Cys(358).

188 The tumor form of pyruvate kinase M2 (PKM2) undergoes tyrosine phosphoryla

189 Recent data indicate that pyruvate kinase M2 (PKM2), a glycolytic enzyme for Warbu

190 key glycolytic proteins, including enolase, pyruvate kinase M2 (PKM2), lactate dehydrogenase and mon

191 ide evidence to support a novel role for the pyruvate kinase M2 (PKM2)-mediated Warburg effect, namel

192 ypes of human tumor cells have overexpressed pyruvate kinase M2 (PKM2).

193 ylation at Y59, which interacts with nuclear pyruvate kinase M2 (PKM2).

194 Here we use short hairpin RNA to knockdown pyruvate kinase M2 expression in human cancer cell lines

195 The pyruvate kinase M2 isoform (PKM2) is expressed in cancer

196 oma (HCC) by maintaining low activity of the pyruvate kinase M2 isoform (PKM2), a key regulator of th

197 s and remarkable downregulation of c-Myc and pyruvate kinase M2 isoform, the key glycolytic enzyme tr

198 IRT6 binds to and deacetylates nuclear PKM2 (pyruvate kinase M2) at the lysine 433 residue.

199 Importantly, pyruvate kinase M2, a fetal anabolic enzyme implicated i

200 lycolytic pathway, 6-phosphofructokinase and pyruvate kinase M2.

201 ark cancer genes, including hexokinase 2 and pyruvate kinase M2.

202 CC cells by targeting hexokinase-2 (Hk2) and pyruvate kinase-M2 (Pkm2).

203 acetylation and lysosomal degradation of the pyruvate kinase-M2 isoform (PKM2).

204 We show that the M2 isoform of pyruvate kinase (M2PYK) exists in equilibrium between mo

205 e for peptides from novel variants of muscle pyruvate kinase, malate dehydrogenase 1, glyceraldehyde-

206 adenovirus-expressed SREBP-1c did not induce pyruvate kinase mRNA, suggesting that induction of this

207 se-transporter-1 mRNA, and of Hexokinase and Pyruvate-Kinase mRNAs, key regulators of glycolysis.

208 xpression to control alternative splicing of pyruvate kinase muscle (PKM) isoforms 1 and 2, resulting

209 Moreover, we show that the metabolic enzyme, pyruvate kinase muscle (PKM), interacts with sub-pools o

210 y in nucleus pulposus (NP) cells through the pyruvate kinase muscle (PKM)-2-Jumonji domain--containin

211 al proximity of Tnfalpha alleles depended on pyruvate kinase muscle isoform 2 (PKM2) and T-helper-ind

212 otein), resulting in alternative splicing of pyruvate kinase muscle isoforms 1 and 2 (PKM1 and 2) and

213 JMJD5 interacts directly with pyruvate kinase muscle isozyme (PKM)2 to modulate metabo

214 Pyruvate kinase muscle isozyme 2 (PKM2) is a key regulat

215 n of hypoxia-inducible factor-1A and reduced pyruvate kinase muscle isozyme 2 activity, both key regu

216 utase 2, glycogen phosphorylase muscle form, pyruvate kinase muscle isozyme, beta-enolase and triosep

217 spots identified as glycogen phosphorylase, pyruvate kinase muscle isozyme, isoforms of creatine kin

218 glucose-6-phosphate dehyrogenase (G6PD) and pyruvate kinase muscle type 2 (PKM2) that facilitates vi

219 ctions to experimental flux data for E. coli pyruvate kinase mutant PB25, we find that MOMA displays

220 NDP kinase to suppress anaerobic growth in a pyruvate kinase-negative E. coli mutant.

221 EGFR and that of a green fluorescent protein-pyruvate kinase-NLS reporter protein.

222 mplex and the structures of two complexes of pyruvate kinase, one with Mg(2+)-bound phospholactate an

223 trol points in this system (e.g. hexokinase, pyruvate kinase, phosphofructokinase, isocitrate dehydro

224 aliana) mutant (pkp1) deficient in plastidic pyruvate kinase (PK(p)) and unable to accumulate storage

225 While in both wild types plastidic pyruvate kinase (PK(p)) provides most of the pyruvate fo

226 Muller glia are deficient for pyruvate kinase (PK) and for aspartate/glutamate carrier

227 Pyruvate kinase (PK) deficiency is the most common cause

228 -opts the cellular glycolytic ATP-generating pyruvate kinase (PK) directly into the viral replicase c

229 Pyruvate kinase (PK) exists in M1 (PKM1) and M2 (PKM2) i

230 shows the critical role of glycolytic enzyme pyruvate kinase (PK) in directing metabolism of prolifer

231 Mammalian pyruvate kinase (PK) is a four-domain enzyme that is act

232 carbon source but the lack of a functioning pyruvate kinase (PK) means that carbohydrates cannot be

233 of the low-activity (dimeric) M2 isoform of pyruvate kinase (PK) over its constitutively active spli

234 In mammals, pyruvate kinase (PK) plays a key role in regulating the

235 sing excess CTP instead of ATP as substrate, pyruvate kinase (PK), and firefly luciferase) to generat

236 duct generates the PKM1 and PKM2 isoforms of pyruvate kinase (PK), and PKM2 expression is closely lin

237 GAPDH, aldolase, phosphofructokinase (PFK), pyruvate kinase (PK), lactate dehydrogenase (LDH), carbo

238 Luo et al. now identify pyruvate kinase (PK)-M2 as an intriguing new interacting

239 tem comprising phosphoenolpyruvate (PEP) and pyruvate kinase (PK).

240 e, whereas another could involve reversal of pyruvate kinase (PK).

241 reen, we identified liver and red blood cell pyruvate kinase (PKLR) as a driver of metastatic liver c

242 ted Sites (BORIS) at the alternative exon of Pyruvate Kinase (PKM) is associated with cancer-specific

243 mely, the rate-limiting enzyme of glycolysis pyruvate kinase (PKM), which plays a critical role in ca

244 d decrease in the expression and activity of pyruvate kinase PKM2, a glycolytic enzyme that indirectl

245 We find that SHMT2 activity limits that of pyruvate kinase (PKM2) and reduces oxygen consumption, e

246 In this study, we identify the M2 isoform of pyruvate kinase (PKM2) as a critical target of the sirtu

247 phorylation of the tumor-specific isoform of pyruvate kinase (PKM2) at Y105, resulting in decreased e

248 Upregulation of the embryonic M2 isoform of pyruvate kinase (PKM2) emerges as a critical player in t

249 active tetramer and inactive dimer forms of pyruvate kinase (PKM2) in cancer cells, similar to the t

250 The M2 isoform of pyruvate kinase (PKM2) promotes the metabolism of glucos

251 e we report that the embryonic M2 isoform of pyruvate kinase (PKM2), a key enzyme contributing to the

252 e M2 isoform of the tightly regulated enzyme pyruvate kinase (PKM2), which controls glycolytic flux,

253 tively express the less active M2 isoform of pyruvate kinase (PKM2).

254 dominance of expression of the M2 isoform of pyruvate kinase (PKM2).

255 a double mutant in two isoforms of plastidic pyruvate kinase (pkpbeta(1)pkpalpha; At5g52920 and At3g2

256 f interleukin-3, suggesting that the nuclear pyruvate kinase plays an important role in cell prolifer

257 axoneme, whereas phosphoglycerate mutase and pyruvate kinase primarily reside in the detergent-solubl

258 ytes activates transcription from the L-type Pyruvate kinase promoter in response to high glucose lev

259 Overexpression of the M2 isoform of pyruvate kinase protein fused with a nuclear localizatio

260 ously established consequences of abolishing pyruvate kinase (Pyk) activity in Escherichia coli durin

261 Pyruvate kinase (PYK) is an essential glycolytic enzyme

262 different conformers of the same species of pyruvate kinase (PYK).

263 olysis and theMtbgenome harbors one putative pyruvate kinase (pykA, Rv1617).

264 on aldehyde-alcohol dehydrogenase (AdhE) and pyruvate kinase (PykF) enzymes, previously not known to

265 t allosteric regulation of the activities of pyruvate kinase (PykF, but not PykA), phosphofructokinas

266 eptide antibodies established that cytosolic pyruvate kinase (PyrKinc) is phosphorylated at both site

267 operation of the phosphoglycerate kinase and pyruvate kinase reactions to enzymatically generate ATP.

268 GAPDH, aldolase, lactate dehydrogenase, and pyruvate kinase revealed not only the anticipated bindin

269 ibility of this approach using rabbit muscle pyruvate kinase (rM1-PK) which catalyzes the conversion

270 in other responsive genes, including l-type pyruvate kinase, S(14) and fatty acid synthase.

271 uvate kinase activity and an increase in the pyruvate kinase substrate phosphoenolpyruvate.

272 s is demonstrated by the ability of GAPDH or pyruvate kinase substrates to directly block the K(ATP)

273 l C-terminal truncation to generate a 51 kDa pyruvate kinase subunit which might have altered regulat

274 d that purified Mck1p does not phosphorylate pyruvate kinase, suggesting that the link is indirect.

275 dynamic movement of the holo form traps the pyruvate kinase tetramer in its enzymatically active sta

276 Here we show thatpykAencodes an active pyruvate kinase that is allosterically activated by gluc

277 These fluxes changed with plastidic pyruvate kinase to maintain a supply of pyruvate for ami

278 Fusions of pyruvate kinase to the lentiviral integrases did not rev

279 ntitative HTS (qHTS), tested with the enzyme pyruvate kinase, to generate concentration-response curv

280 ed glucose activation of both GRBP and liver pyruvate kinase transcription.

281 d activation of fructose-1,6-bisphosphate on pyruvate kinase translate flux information into the conc

282 are proposed to explain how Escherichia coli pyruvate kinase type 1 is allosterically regulated: the

283 our cell exosomes secretion is controlled by pyruvate kinase type M2 (PKM2), which is upregulated and

284 versible aggregation of the metabolic enzyme pyruvate kinase under environmental stress and propose a

285 vely, fructose 1,6-bisphosphate aldolase and pyruvate kinase, under the same conditions.

286 e consequence of an allosteric activation of pyruvate kinase via cytosolic NAD+ content.

287 d nuclear translocation of the M2 isoform of pyruvate kinase was dependent on the activation of Jak2.

288 hosphate isomerase was up-regulated, whereas pyruvate kinase was down-regulated.

289 In addition, the glycolytic enzyme pyruvate kinase was found to be phosphorylated by PrkD o

290 First, green fluorescent protein or pyruvate kinase was fused to EKLF domains, and localizat

291 The C domain of the M2 isoform of pyruvate kinase was sufficient for interleukin-3-induced

292 ermediate in the reaction catalyzed by yeast pyruvate kinase, was investigated by site-directed mutag

293 peroxide dismutase and the glycolytic enzyme pyruvate kinase were deficient in an mntH strain grown u

294 atine kinase, 3-phosphoglycerate kinase, and pyruvate kinase were evaluated using preparations from c

295 r neurons express AGC1 and the M2 isoform of pyruvate kinase, which is commonly associated with aerob

296 monovalent cation with wild type (WT) yeast pyruvate kinase (YPK) and with the T298S, T298C, and T29

297 inants of the allosteric activation of yeast pyruvate kinase (YPK) by mutational and kinetic analysis

298 llosteric response of Mg(2+)-activated yeast pyruvate kinase (YPK) during steady-state turnover were

299 Yeast pyruvate kinase (YPK) is regulated by intermediates of t

300 The active site T298 residue of yeast pyruvate kinase (YPK), located in a position to serve po