ライフサイエンスコーパス: qPCR

1 qPCR analysis indicated that BmAce1 is highly expressed

2 qPCR and dPCR quantification of enterococci in the 24 en

3 qPCR and TaqMan qPCR were used to assess chondrogenic ge

4 qPCR has established itself as the technique of choice f

5 qPCR revealed a decrease in expression of miR-451a in NR

6 qPCR was performed to measure the expression level of tr

7 qPCR,luciferase reporter assays, and western blot also v

8 mples were tested by 6 laboratories using 10 qPCR assays calibrated to the IS.

9 A qPCR time course of (1)O2 induced systemic marker genes

10 e ARG and MGE abundance was measured using a qPCR array with 363 primer pairs.

11 tion and 85.1% (95% CI, 26.5%-97.0%) using a qPCR-based case definition.

12 Alternatively, qPCR requires less DNA and is potentially simpler to per

13 n measured with the short and long amplicons qPCR, respectively.

14 cal analysis revealed smaller adipocytes and qPCR analysis showed upregulated expression of some adip

15 ry; and GCS efficacy by apoptosis assays and qPCR.

16 lso, we have identified by bioinformatic and qPCR analysis, different miR expression patterns in colo

17 coplasma hominis was detected by culture and qPCR in 2 unused vials from the donor.

18 al pneumococcal colonization (by culture and qPCR) can be used as a method of microbiological diagnos

19 small phytoplankton using flow cytometry and qPCR assays for phylogenetically distinct Bathycoccus cl

20 ddPCR and qPCR-Std-curve yielded similar predictions for Vgsc CN,

21 Here, Droplet Digital PCR (ddPCR) and qPCR platforms were directly compared for gene expressio

22 NA degradation using gel electrophoresis and qPCR with both short amplicons ( approximately 200 bps,

23 by immunoblotting, immunohistochemistry, and qPCR.

24 tion, the agreement between positive LFD and qPCR results was 42.3% and 50% for BacT/Alert and MGIT l

25 Further analysis by microarray and qPCR indicated that the expression of FT is down-regulat

26 a was carried out using light microscopy and qPCR.

27 trated an alteration in the Wnt pathway, and qPCR confirmed an increased expression of secreted frizz

28 itive diarrhea samples identified by PCR and qPCR and five C. difficile-negative diarrhea controls we

29 ositive with both immune tissue printing and qPCR; whereas 95% were positive with at least one of the

30 hits using bisulfite PCR pyrosequencing and qPCR.

31 RNAseq and qPCR confirmed significant differences in mean expressio

32 ene expression was measured using RNASeq and qPCR.

33 a strong correlation between the RNA-Seq and qPCR results.

34 eal taxa was assessed by iTag sequencing and qPCR gene assays of samples spanning an oxic-anoxic-euxi

35 In cord blood, qPCR, TESA-blot, and micromethod sensitivities/specifici

36 ssion of EGFR and RELA was validated by both qPCR and immunoblotting and they were both shown to be u

37 Furthermore, we analysed by BuV qPCR stool and nasal swab samples from 955 children with

38 By qPCR and immunoblot analysis we verified that the nuclea

39 By qPCR, pneumococcal colonization was detected in 10 LRTI

40 HIV DNA was detected in 18 by qPCR and in 15 by dPCR, the difference being due to the

41 we tested diarrheal specimens during 2015 by qPCR for a broad range of enteropathogens and calculated

42 profiles, 19 genes were further analysed by qPCR.

43 Analysis by qPCR of 271 porcine respiratory disease diagnostic submi

44 s in humanized mouse livers were analyzed by qPCR and Nanostring.

45 diction using ingenuity pathway (IPA) and by qPCR analyses.

46 ected by IHC, hydroxyproline content, and by qPCR for fibrotic markers.

47 ential miR-184 target genes was confirmed by qPCR and 3'UTR luciferase reporter assay.

48 nd fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC).

49 ared with HCs, 31 (79.48%) were confirmed by qPCR corresponding to genes involved in epidermal homeos

50 icantly dysregulated, which was confirmed by qPCR.

51 s detected by 16S profiling and confirmed by qPCR.

52 ne receptor (HR) expression were detected by qPCR and HA secretion by enzymatic immunoassay.

53 asmodium falciparum prevalence determined by qPCR decreased from 42% in 2006 to 9% in 2014.

54 d galectin-10 mRNA levels were determined by qPCR.

55 vivax parasitaemia and 10% P. falciparum, by qPCR, both of which were predominantly sub-microscopic a

56 f GHRH and its receptor (GHRH-R) measured by qPCR and Western blotting were significantly down-regula

57 on but also easily validated and measured by qPCR techniques.

58 ic and inflammatory factors were measured by qPCR, ELISA, and Western blotting.

59 (HUVECs) resembling EndoMT were monitored by qPCR, immunocytochemistry and western blots.

60 sitive for M. tuberculosis complex (MTBC) by qPCR.

61 from the raw amplification data produced by qPCR-based technologies.

62 e correlated with HOL1 genotype, as shown by qPCR and RT-qPCR.

63 eases are unlikely to be explained solely by qPCR measurement error.

64 lood of donors and recipients were tested by qPCR for the presence of BKV DNA before and after transp

65 Cys-loop gene expression in muscle tissue by qPCR and localized this expression in mechanosensilla vi

66 Thirteen transcripts were validated by qPCR to confirm cell specific expression in microdissect

67 miR-23a-5p and miR-23b-5p) were validated by qPCR.

68 -reads sequencing, copy number variations by qPCR, RNA concentrations by qRT-PCR, and protein concent

69 tially expressed in the skin (as verified by qPCR) and had not been previously identified in potato w

70 With single-cell qPCR, we temporally examined CM gene expression and obse

71 ChIP-qPCR assay showed that pCREB enrichment on the C/EBPbeta

72 quences were independently validated by ChIP-qPCR (quantitative PCR), oligonucleotide-binding assays

73 n immunoprecipitation quantitative PCR (ChIP-qPCR) indicate that in LCLs inhibition of CDKN2C (p18INK

74 site in the murine Dnajc22 locus using ChIP-qPCR and luciferase assays and verified this regulatory

75 itative polymerase chain reaction (DNase-CMV-qPCR) was developed to differentiate free naked DNA from

76 all 10 fresh samples tested using DNase-CMV-qPCR.

77 sponses comparable with that of a commercial qPCR instrument.

78 25 viral qPCR-negative subjects, to compare qPCR with sequencing-based virus detection and to genera

79 A higher 'composite-qPCR vaginal-health-score' was directly associated with

80 Procedures for conducting qPCR have received significant attention; however, more

81 ques, respectively, compared to conventional qPCR positivity rates of 0%, 0%, 30%, and 100% and CFU d

82 d on initial screening, two novel crAssphage qPCR assays (CPQ_056 and CPQ_064) were designed and eval

83 Findings confirm that viral crAssphage qPCR assays perform at a similar level to well-establish

84 However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TG

85 , we performed high-throughput single embryo qPCR analysis in Cdx2 null embryos to identify CDX2-regu

86 Gene expression (qPCR) analysis and immunohistochemistry of the palmar ep

87 SNORD44 as a suitable housekeeping gene for qPCR analysis comparing normal and cancer cells.

88 We selected 17 genes for qPCR to validate RNA-Seq data.

89 LED)-based epifluorescence sensor module for qPCR sensor development and relevant bioassay applicatio

90 l's effect on BAT was assessed by histology, qPCR, HPLC, LC/MS and measures of core body temperature.

91 By combining imaging, qPCR and experimental xenodiagnoses with mathematical mo

92 Chromatin-Immunoprecipitation-qPCR and electrophoretic mobility shift assays showed th

93 results because of retesting prompted by LSG-qPCR positivity.

94 oach based on the SYBR green technology (LSG-qPCR), which uses genus-specific primers that target the

95 On the basis of the Tm values of the LSG-qPCR amplicons from reference and clinical specimens, we

96 ed positive for Leishmania spp. with the LSG-qPCR assay, specimens from 465 of these 477 patients als

97 the amplicons on qPCR platforms (the Mx3000P qPCR system [Stratagene-Agilent] and the 7500 real-time

98 nesis (shown in TCGA) were increased in NRs (qPCR).

99 but on average dPCR values were only 60% of qPCR values.

100 us approaches to the statistical analysis of qPCR data are needed.

101 rmed the Common Base Method, for analysis of qPCR data based on threshold cycle values (C q ) and eff

102 The applicability of qPCR in olive-oil authentication depends on the DNA obta

103 c sample prep to reduce the co-extraction of qPCR inhibitors with the amplification of two MTB specif

104 is of MG secretions, we analyzed by means of qPCR, the expression levels of six of the CYP450 genes m

105 priate reference miRNAs for normalization of qPCR data is crucial for accurate expression analysis.

106 We compared the performance of qPCR and dPCR in quantifying HIV DNA in 18 patient sampl

107 to ensure the robustness and reliability of qPCR data for analyzing miR expression.

108 r reaction, only 3 times higher than that of qPCR.

109 melting temperature (Tm) of the amplicons on qPCR platforms (the Mx3000P qPCR system [Stratagene-Agil

110 ulation from clade IIC to clade IA, based on qPCR monitoring of polyphosphate kinase 1 (ppk1) gene va

111 cally relevant cutoff of >8,000 copies/ml on qPCR, pneumococcal colonization was found in 3 LRTI pati

112 was further confirmed by western blot and/or qPCR.

113 le to those obtained by conventional LAMP or qPCR approaches.

114 ained with the different approaches, overall qPCR proved more sensitive than qLAMP.

115 Three sets of paired qPCR (P-qPCR) assays with amplicons of variable length were used

116 Three sets of paired qPCR (P-qPCR) assays with amplicons of variable length w

117 levels were determined by quantitative PCR (qPCR) (p = 1.8 x 10(-20)) and in two cohorts where HLA-C

118 immunoprecipitation (ChIP)-quantitative PCR (qPCR) analysis of their promoters revealed decreased H3K

119 ere, ddPCR was compared to quantitative PCR (qPCR) and pyrosequencing.

120 ; and gene expression with quantitative PCR (qPCR) and RNA sequencing on lung epithelium and mesenchy

121 describe a novel real-time quantitative PCR (qPCR) approach based on the SYBR green technology (LSG-q

122 o that with a conventional quantitative PCR (qPCR) assay and blood culture.

123 -R test, a rapid real-time quantitative PCR (qPCR) assay that detects five families of carbapenemase

124 ulturable counts (CFU) and quantitative PCR (qPCR) cell equivalent counts of Escherichia coli in dair

125 Using quantitative PCR (qPCR) for common respiratory viruses and for two genes k

126 erase chain reaction (PCR)/quantitative PCR (qPCR) for Streptococcus mutans and Streptococcus sobrinu

127 Quantitative PCR (qPCR) has become the gold standard technique to measure

128 Real-time quantitative PCR (qPCR) is one of the most powerful techniques for analyzi

129 n was also consistent with quantitative PCR (qPCR) measurements as well as cell counts for BioBall re

130 uantify reproducibly using quantitative PCR (qPCR) or next generation sequencing (NGS) due to the pre

131 16S sequencing/quantitative PCR (qPCR) revealed significant changes in phyla abundance, p

132 In experiment 1, quantitative PCR (qPCR) was performed at several stages to determine trans

133 microscopy (micromethod), quantitative PCR (qPCR), and immunoglobulin (Ig)M trypomastigote excreted-

134 Using quantitative PCR (qPCR), droplet digital PCR, and fluorescent in situ hybr

135 using microarray analysis, quantitative PCR (qPCR), enzyme-linked immunosorbent assay (ELISA), and We

136 Its advantages over quantitative PCR (qPCR), including absolute quantification without a stand

137 that were PCV3 positive by quantitative PCR (qPCR), with 60% of a subset also testing positive for PC

138 cted with VZV using TaqMan quantitative PCR (qPCR).

139 S rRNA gene sequencing and quantitative PCR (qPCR).

140 iRNA followed by real-time quantitative PCR (qPCR).

141 re specifically, quantitative real time PCR (qPCR) achieves a high degree of sensitivity, although th

142 was confirmed by quantitative real-time PCR (qPCR) analysis, suggesting their specific physiological

143 DNA amplification techniques, real-time PCR (qPCR) and real-time Loop-mediated isothermal AMPlificati

144 We analyzed quantitative real-time PCR (qPCR) assays on DNA extracted from these specimens to qu

145 ased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and labor

146 confirmed by the quantitative real-time PCR (qPCR), droplet digital PCR (ddPCR), and Sanger sequencin

147 omputed tomography (muCT) and real time-PCR (qPCR) analyses, individual trabecula segmentation (ITS)-

148 culture (spoligotyping or quantitative PCR [qPCR]) in each laboratory; liquid (MGIT or BacT/Alert) a

149 ity and progression biomarkers, we performed qPCR on a set of 16 animal model-derived potential bioma

150 icin-B therapy initiated based on a positive qPCR.

151 Prior qPCR results for nuwts showed similar correlations for b

152 March 2016 was conducted using quantitative qPCR.

153 and quantitative polymerase chain reaction (qPCR) analyses of Mkx(-/-) PDL revealed an increase in o

154 Quantitative polymerase chain reaction (qPCR) analysis instead revealed that the sum of bacteria

155 ting quantitative polymerase chain reaction (qPCR) assays of U.S. Environmental Protection Agency Met

156 s or quantitative polymerase chain reaction (qPCR) experiments.

157 time quantitative polymerase chain reaction (qPCR) for 21 candidate genes.

158 the quantitative polymerase chain reaction (qPCR) method.

159 and quantitative polymerase chain reaction (qPCR) of functional genes in the denitrification pathway

160 time quantitative polymerase chain reaction (qPCR) sensors.

161 itative real-time polymerase chain reaction (qPCR), and whole-genome sequencing were performed.

162 itative real-time polymerase chain reaction (qPCR), relevant genes expressed in nonlesional (NLS-CSU)

163 as a quantitative polymerase chain reaction (qPCR)-based etiology study at a rural Tanzanian hospital

164 d using real-time polymerase chain reaction (qPCR).

165 of biofilm material (determined by 16S rRNA qPCR) surrounding the released L. pneumophila.

166 RT-qPCR analyses of patients' primary fibroblasts and lucif

167 RT-qPCR analysis revealed that TrkC-miR2 is significantly u

168 RT-qPCR data revealed that AgNPs induced greater changes in

169 RT-qPCR showed reduced Spr1 mRNA levels in all transformant

170 RT-qPCR suggests model specific gene expression for nine pu

171 RT-qPCR verified model specific and nonspecific expression

172 ultrafiltration, gel electrophoresis, and RT-qPCR (quantitative reverse transcription polymerase chai

173 ed using Illumina-based transcriptome and RT-qPCR analyses.

174 RNA-seq and RT-qPCR identified potential downstream genes of SPL4.

175 ne expression was examined by RNA-Seq and RT-qPCR in order to understand the molecular mechanisms of

176 (SSH) followed by 454 pyrosequencing, and RT-qPCR methods.

177 Infectivity and RT-qPCR reductions are also presented for surrogate viruses

178 ed to the discrepancy between the MS- and RT-qPCR-based results.

179 massive analysis of cDNA ends (MACE) and RT-qPCR.

180 image analysis, immunoprecipitation, and RT-qPCR.

181 with HOL1 genotype, as shown by qPCR and RT-qPCR.

182 s, as confirmed by immunofluorescence and RT-qPCR.

183 ely on reverse transcription (RT) such as RT-qPCR and RNA-Seq.

184 nation detected by our novel TaqMan-based RT-qPCR assay, with POS1 performing the best.

185 gene expression changes were verified by RT-qPCR and ELISA.

186 innate immune responses were assessed by RT-qPCR and IFN protein release by ELISA.

187 Viral replication was determined by RT-qPCR and virion release by TCID50 assay.

188 the TaqMan Low Density Array followed by RT-qPCR confirmation.

189 t genotypes and organs, were confirmed by RT-qPCR Corresponding target transcripts, predicted in sili

190 most significantly deregulated miRNAs by RT-qPCR in an independent sample set.

191 Gene expression analysis by RT-qPCR revealed enrichment of p53 signaling and extracellu

192 Gene expression was detected by RT-qPCR, and protein production or activity was determined

193 ucocorticoid-mediated ST2 was assessed by RT-qPCR, ChIP assay and luciferase reporter assay.

194 pts, predicted in silico and validated by RT-qPCR, often showed opposite expression profiles than the

195 mously across a graft union identified by RT-qPCR, RNA gel blot, and in situ RT-PCR analyses.

196 milation were the key genes, validated by RT-qPCR, which expressed in a network manner with tissue sp

197 ecursor nor mature product is detected by RT-qPCR.

198 ality intact RNA suitable for single-cell RT-qPCR as well as RNA-Seq, enabling the reliable detection

199 nsequently we propose they be adopted for RT-qPCR experiments involving this pathosystem.

200 ateness of reference genes is crucial for RT-qPCR.

201 rn blots) were used as reference genes in RT-qPCR experiments, but recent studies in different system

202 monochloramine with CT values for 2 log10 RT-qPCR reduction between 300 and 360 mg-min/L.

203 The MeVA RT-qPCR assay has been used successfully for measles survei

204 tandard real-time RT-PCR method, the MeVA RT-qPCR showed 99.5% specificity for genotype A and 94% sen

205 (5) J/m(2) UVA), neither MALDI-TOF-MS nor RT-qPCR detected significant decreases in the oligomer conc

206 the combination of clinical observations, RT-qPCR in multiple diagnostic specimens, and serology are

207 o guarantee satisfactory normalization of RT-qPCR data when using DNBS-Model.

208 On the basis of RT-qPCR data, electrostatic interactions and an ion-exchang

209 A-infected animals and the variability of RT-qPCR results among specimen type demonstrated that a dia

210 Based on RT-qPCR validations, eXpress and Multi-Mapping Bayesian Gen

211 antification of HRV, RT-dPCR outperformed RT-qPCR by consistently and accurately quantifying HRV RNAs

212 h reverse transcription quantitative PCR (RT-qPCR) and quantitative matrix-assisted laser desorption-

213 f reverse transcription-quantitative PCR (RT-qPCR) and reverse transcription-digital PCR (RT-dPCR) fo

214 e reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RN

215 e quantitative reverse transcriptase PCR (RT-qPCR) from nine human cell lines and RNA-seq data availa

216 on assays, real-time quantitative RT-PCR (RT-qPCR), and flow cytometry.

217 mutagenesis, real-time quantitative PCR (RT-qPCR), and Western blotting analysis.

218 h reverse transcription-quantitative PCR (RT-qPCR), is a common approach employed to investigate the

219 A reverse transcription-quantitative PCR (RT-qPCR)-based assays and from outgrowth assay readout by f

220 y reverse transcriptase quantitative PCR (RT-qPCR).

221 es virus (MeV) (MeVA RT-quantitative PCR [RT-qPCR]) that can identify measles vaccine strains rapidly

222 s assessed by reverse transcription-qPCR (RT-qPCR).

223 e-quantitative polymerase chain reaction (RT-qPCR) analysis and by sequencing of PCR-amplified bisulf

224 n quantitative polymerase chain reaction (RT-qPCR) assay for quantification.

225 tive real-time polymerase chain reaction (RT-qPCR) assays and a thorough statistical analysis was con

226 e quantitative polymerase chain reaction (RT-qPCR) assays in Gabonese children with severe (n = 184)

227 tive real-time-polymerase chain reaction (RT-qPCR) experiments again of the same individual, providin

228 n-quantitative polymerase chain reaction (RT-qPCR) platforms for fold change estimates than for raw a

229 transcription polymerase chain reaction (RT-qPCR) were used to identify regulators of bud formation.

230 tive real-time polymerase chain reaction (RT-qPCR).

231 Broadly reactive RT-qPCR primers targeting HEV ORF2/3 were successfully adap

232 During colonisation of rice, RT-qPCR analyses showed that H. rubrisubalbicans up-regulat

233 technically validated using gold-standard RT-qPCR.

234 achieved the highest correlation with the RT-qPCR and exon-array (MMBGX) results overall, RSEM was mo

235 tatistical analysis was conducted for the RT-qPCR data.

236 stance to inhibitors than a commonly used RT-qPCR assay.

237 lidated in additional tumor samples using RT-qPCR and pyrosequencing.

238 from patients and healthy controls using RT-qPCR and receiver operating characteristic (ROC) analysi

239 Gene expression was analyzed using RT-qPCR and/or enzyme-linked immunosorbent assay in T-cell

240 s 2.0 and performance was validated using RT-qPCR.

241 ACE data, candidate genes were tested via RT-qPCR and a strong positive correlation between both data

242 easurement of four unique RNA signals via RT-qPCR.

243 plicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations.

244 In WW, RT-qPCR reductions were less than 0.5 log10 for all viruses

245 ng this sample preparation method, sensitive qPCR detection of the extracted S. aureus bacterial DNA

246 Transcriptome (RNA-seq, qPCR, sRNA-seq, and PARE) and methylome profiling during

247 16S rRNA cDNA sequencing, qPCR of mcrA transcripts, and functional gene array-base

248 Archaea-specific qPCR yielded archaea in 8/18 brain abscess specimens and

249 However, our SSH, qPCR and enzymatic activity assays indicated that 5'-nuc

250 plexing, all while requiring only a standard qPCR instrument for readout.

251 specificity of XNA polymerases in a standard qPCR instrument.

252 qPCR and TaqMan qPCR were used to assess chondrogenic gene and miRNA exp

253 l noncoding RNAs was also verified by TaqMan qPCR in productively infected fibroblasts and ARPE19 cel

254 For use in routine analysis, derived TaqMan qPCR methods for events 16-0-1 and 18-2-4 were developed

255 Overall, our results demonstrate that qPCR conservatively measures the potential for a gene to

256 Results indicate that qPCR significantly increases detection of S. pneumoniae,

257 The qPCR conditions, primer concentration and annealing temp

258 The qPCR threshold cycle for direct PCR from whole blood is

259 d SPME of DNA from a dilute cell lysate, the qPCR amplification efficiency was determined to be 100.3

260 and p value <0.0001); the group mean of the qPCR measurements was 0.19 log units higher than that of

261 DA results were consistent with those of the qPCR reference.

262 CCL8/CXCL11-high subjects and in two of the qPCR-negative subjects.

263 concentrations, a 50-fold improvement on the qPCR assay used routinely in the clinic.

264 he length of the entire pWH1266 plasmid, the qPCR rate constants were 2-7x larger than the rate const

265 ve a higher rate of detection (83%) than the qPCR method (64%).

266 predictions for Vgsc CN, indicating that the qPCR protocol developed here can be applied as a diagnos

267 This qPCR system enabled detection, with high sensitivity and

268 SSR markers and Real Time qPCR analysis showed that different substrates promoted

269 Real-time qPCR has confirmed the differential life-cycle stage exp

270 transgenic plants, as assessed by real-time qPCR, and accompanied by the production of reactive oxyg

271 Real-time qPCR, used for quantification of this reservoir by measu

272 HdT-exclusive genes to validate by real-time qPCR, which most of them confirmed their higher expressi

273 Compared to qPCR confirmation, the agreement between positive LFD an

274 ntages and disadvantages of dPCR compared to qPCR, its applications in clinical microbiology, and con

275 on achieved 86% sensitivity when compared to qPCR-based screening.

276 A comparison to qPCR results across the chlorine disinfection step saw n

277 g 325 samples to adapt the neural network to qPCR measurements, the model was validated using 51 inde

278 tes were quantified by reverse-transcription qPCR analysis.

279 er (PB) as assessed by reverse transcription-qPCR (RT-qPCR).

280 ere, using a sensitive reverse transcription-qPCR approach, we demonstrate the carriage of eight sign

281 We use qPCR to measure relative telomere length in 389 blood sa

282 Using qPCR and soils archived since 1923 at Askov Experimental

283 mail, and tested them for Bd and Bsal using qPCR.

284 unique challenges of quantifying ctDNA using qPCR to allow observations of real-time tumor dynamics.

285 validated in 42 primary neuroblastomas using qPCR.

286 ility of this signature was reproduced using qPCR analysis of an independent cohort of neuroblastomas

287 r detectability and predictive potential via qPCR in whole blood.

288 d detection of low abundance transcripts via qPCR.

289 We found 21 viral qPCR-positive and 2 suspected virus-infected subjects wi

290 ow to these subjects, together with 25 viral qPCR-negative subjects, to compare qPCR with sequencing-

291 neys (cisplatin) in vivo and ex vivo, whilst qPCR, Taqman low-density array and immunoblot analysis o

292 along with its evaluation in comparison with qPCR.

293 riments on simulated data and real data with qPCR validation.

294 Finally, comparison of dPCR with qPCR results on clinical samples demonstrated the potent

295 RNA-Seq with qPCR was used to indicate genes involved in plant defens

296 U, a selection of 142 genes was studied with qPCR, and 103 (72.53%) were confirmed.

297 C-X-C motif) ligand 14 (CXCL14) to test with qPCR in a larger set of placentas (n = 475) and found no

298 transcriptome profiling were validated with qPCR and flow cytometry assays.

299 ng microArray profiling, were validated with qPCR.

300 e Cytosponge device for microbial DNA yield (qPCR), diversity, and community composition.