ライフサイエンスコーパス: qRT-PCR

1 qRT-PCR analyses revealed that PnSERK2 was expressed at

2 qRT-PCR analyses revealed the expression profiles of the

3 qRT-PCR analyses showed a down-regulation of neurotrophi

4 qRT-PCR analysis confirmed the microarray results, that

5 qRT-PCR analysis demonstrated an increased expression of

6 qRT-PCR analysis of GmSNAPs indicates a co-regulation fo

7 qRT-PCR analysis revealed that only expression of acid c

8 qRT-PCR analysis revealed tissue-specific and hormone-re

9 qRT-PCR analysis using independent tissue samples confir

10 qRT-PCR and protease activity assays demonstrated that u

11 qRT-PCR assay detected higher miR-214 expression in the

12 qRT-PCR assay indicated that compound 1 and DBL exposure

13 qRT-PCR data showed low levels of collagen I and V, as w

14 qRT-PCR experiments revealed that TcSOD2 was present thr

15 qRT-PCR expression analysis of osteoblast and fibroblast

16 qRT-PCR indicated that all DR subtypes were expressed by

17 qRT-PCR of pathogen miRNAs isolated from extracellular v

18 qRT-PCR results on 25 multi-isoform genes in a stem cell

19 qRT-PCR results were consistent with the motility phenot

20 qRT-PCR studies on the expression of genes known to be i

21 qRT-PCR studies revealed the highest RbTI expression in

22 qRT-PCR was performed to validate the expression of the

23 qRT-PCR was used to measure Rpe65 mRNA levels.

24 qRT-PCR, Western blotting, and enzymatic assays were per

25 qRT-PCR-based measurements revealed multifold inaccuraci

26 A qRT-PCR analysis confirmed the dsRNA-mediated transcript

27 A qRT-PCR analysis confirmed the expression profiles of se

28 Here, we have used microarrays and a qRT-PCR primer platform covering 1,880 genes encoding tr

29 authorization for the test, which excluded a qRT-PCR reference method threshold cutoff, were a PPA of

30 d from serum samples of 156 patients using a qRT-PCR array for 22 miRNAs.

31 quantitative reverse transcription analysis (qRT-PCR), and semiquantitative methods, such as microarr

32 xyresorufin-O-deethylase (EROD) activity and qRT-PCR, and cell cycle was measured by flow cytometry.

33 xpression based on fundus image analysis and qRT-PCR.

34 ated using the TaqMan low-density arrays and qRT-PCR.

35 Immunohistochemistry, Western blot, and qRT-PCR were performed on the outflow vein at 7 and 21 d

36 ive in vitro assays using flow cytometry and qRT-PCR analyses and in vivo assays to determine acute t

37 We used flow cytometry and qRT-PCR to screen fetal mouse-derived neurosphere cultur

38 stimulated cells by using flow cytometry and qRT-PCR, respectively.

39 nts, CD11b-based retinal flow cytometry, and qRT-PCR analysis of key microglia markers.

40 ssection of autopsy human hippocampus DG and qRT-PCR miRNA analyses were combined with immunofluoresc

41 tern blotting, immunofluorescence, ELISA and qRT-PCR, we investigated the production of transthyretin

42 The limit of detection for FilmArray and qRT-PCR assays with inactivated ZEBOV, based on duplicat

43 Rates of agreement between FilmArray and qRT-PCR results for clinical specimens from patients wit

44 The agreement for FilmArray and qRT-PCR results using contrived whole-blood specimens wa

45 Cell fractionation and qRT-PCR analysis indicated that LINC00152 is found mainl

46 Immunohistochemistry and qRT-PCR confirmed hippocampal FKBP1b overexpression 4-6

47 nce (kappa of >/=0.723) with the RT-LAMP and qRT-PCR assays in detecting the dengue viremic samples.

48 genesis, immunoprecipitation, luciferase and qRT-PCR-based signaling assays, confocal microscopy and

49 downregulation of ADAR2 both in metadata and qRT-PCR based validation.

50 d in-depth analysis including microarray and qRT-PCR (polymerase chain reaction).

51 Microarray and qRT-PCR analysis of human hair follicles after Nrf2 acti

52 the microarray dynamic range, microarray and qRT-PCR were quantitatively reproducible, including mini

53 were seen in mRNA expression (microarray and qRT-PCR).

54 The microarrays and qRT-PCR resulted in similar gene-expression changes, con

55 analysis, laser capture microdissections and qRT-PCR to evaluate axonal density and Nogo-A protein an

56 Confocal microscopy and qRT-PCR were used to visualize and quantitate successful

57 RT-PCR and qRT-PCR were performed to confirm our findings by detect

58 orescence staining, RT-PCR, and qRT-PCR, and qRT-PCR analysis revealed increased transcriptional indu

59 of immunofluorescence staining, RT-PCR, and qRT-PCR, and qRT-PCR analysis revealed increased transcr

60 st or human DBR1 enzyme prior to 5' RACE and qRT-PCR.

61 RNA-seq and qRT-PCR analyses show that Mr-OPY2 is a negative regulat

62 RNA-seq and qRT-PCR expression analysis showed that overexpression o

63 in MDV-infected CD4+ T cells by RNA-Seq and qRT-PCR.

64 ression arrays, strand-specific RNA-seq, and qRT-PCR experiments.

65 RNA sequencing and qRT-PCR revealed that arginine biosynthesis genes (argR,

66 Next-generation sequencing and qRT-PCR were used to measure plasma microRNAs.

67 n-DENV RT-iiPCR, NS1 antigen rapid test, and qRT-PCR tests was 93.9%, 84.5%, and 97.4%, respectively,

68 FAT isoforms were treated with TNFalpha, and qRT-PCR was used to examine the contribution of each iso

69 -1) by UDCA was studied using zymography and qRT-PCR.

70 AID protein within variably divided blasts, qRT-PCR of p53 mRNA from cultures with or without actino

71 can and Gambian cohorts (p values <0.0001 by qRT-PCR) with a sensitivity of 53.7% (42.6-64.3) and a s

72 Validation analysis by qRT-PCR showed significant upregulation of only miR-455-

73 ndergoing bariatric surgery were analyzed by qRT-PCR for expression of WNT/PCP genes.

74 pha2 ratio was determined by SDS PAGE and by qRT-PCR in ex-vivo bone explants.

75 cted mucosa-associated genes was assessed by qRT-PCR in CD and HC children and in treated CD adults (

76 expression was clarified in immune cells by qRT-PCR, and timing of expression was tested in islets u

77 pression was confirmed in a larger cohort by qRT-PCR.

78 er variations by qPCR, RNA concentrations by qRT-PCR, and protein concentrations by immunoblotting.

79 the aforementioned stresses was confirmed by qRT-PCR analysis in distinct Arachis genotypes, whilst i

80 roscopy, and knockdown of AnxA2 confirmed by qRT-PCR and Western blot.

81 gets ZEB1/2, GATA2, and KDR was confirmed by qRT-PCR as being lower in obese patients with periodonti

82 ges detected by microarray were confirmed by qRT-PCR with high concordance.

83 The expression of hub genes confirmed by qRT-PCR, ELISA (IL-6, IL-1beta, and CXCL2), and Western

84 RNAs and their targets was also confirmed by qRT-PCR.

85 We demonstrate by qRT-PCR and confocal imaging that mouse Abcg4 is express

86 ds, with one additional specimen detected by qRT-PCR below the limits of the DFA sensitivity.

87 imidine biosynthesis genes were evaluated by qRT-PCR during infection in Solanum phureja.

88 Gene expression levels were evaluated by qRT-PCR using RNA isolated from tumor tissue and blood.

89 We measured SPARC expression by qRT-PCR in human primary pancreatic islets, adipose tiss

90 ith recombinant IL-13 and gene expression by qRT-PCR was performed for collagen1A1 and TGF-beta1.

91 etric quantification and sorting followed by qRT-PCR, and to DNA methylation analyses of the Treg-spe

92 We found by qRT-PCR that AtxA1 and AtxA2 function as positive regula

93 ssion was analyzed 4 weeks post-injection by qRT-PCR and histology.

94 otential in an independent cohort of LUAD by qRT-PCR.

95 fold higher in ATII cells than whole lung by qRT-PCR, and was undetectable in other viscera.

96 le expression of alr1105 gene as measured by qRT-PCR at different time points under selected stresses

97 Expression levels were measured by qRT-PCR in leukocytes from 1846 Framingham Heart Study p

98 However, ssRNA cleavage measured by qRT-PCR underestimated inactivation over longer duration

99 A levels of approximately 40% as measured by qRT-PCR-based miRNA profiling.

100 d class switch recombination was measured by qRT-PCR.

101 transcriptional regulation were measured by qRT-PCR.

102 uantify Bmal1, Per1, Per2, and Nr1d1 mRNA by qRT-PCR.

103 was confirmed for eight of these networks by qRT-PCR in an independent set of term and pre-term subje

104 mArray testing in four specimens and only by qRT-PCR testing in the remaining four specimens.

105 ession of 29 candidate miRNAs in placenta by qRT-PCR.

106 s from three patients were found positive by qRT-PCR for ZIKAV and the viral RNA copy numbers detecte

107 evels of VEGF and VEGFR-2 were quantified by qRT-PCR and showed significant reduction in message expr

108 ed genes in these T cells were quantified by qRT-PCR.

109 for the expression of the oxytocin system by qRT-PCR, in situ hybridization, receptor autoradiography

110 sting and matched plasma specimens tested by qRT-PCR testing, and 85% (11/13 specimens) for urine spe

111 s of IFN-I in blood and lymph node tissue by qRT-PCR.

112 35 hepatocellular carcinoma (HCC) tissues by qRT-PCR.

113 selected candidate miRNAs were validated by qRT-PCR analysis of cohorts of 24 T1DM and 24 control su

114 t role on altered pathways were validated by qRT-PCR analysis on 12 samples per group.

115 ors (emx2, lhx2, and hopx), was validated by qRT-PCR and immunostaining in brain sections.

116 L1, TPD52, IQCG, and ACOX2 were validated by qRT-PCR in a third cohort of 40 ER+ HER2- and ER- HER2-

117 on of four novel small RNAs was validated by qRT-PCR in human skin, and their Argonaute association w

118 nges identified by RNA-Seq were validated by qRT-PCR open arrays.

119 iRNAs, among which miR-200b was validated by qRT-PCR to be significantly increased in obesity.

120 different S. mussotii tissues, validated by qRT-PCR, and compared with the homologous genes from S.

121 AGL9, LRR, PKL and ARF8-1 were validated by qRT-PCR.

122 xpression of miRNA and mRNA was validated by qRT-PCR.

123 iles of these eight miRNAs were validated by qRT-PCR.

124 n CPT biosynthetic pathway were validated by qRT-PCR.

125 ene expression in the apples was verified by qRT-PCR.

126 ter harvest at 20 degrees C and 4 degrees C. qRT-PCR results were supported by correlation analysis w

127 ingle molecule FISH (smFISH) and single cell qRT-PCR (or single cell RNA-seq).

128 Single cell qRT-PCR analysis showed that the combination of IFN-gamm

129 Using single-cell qRT-PCR and clonal analyses, here we define four subpopu

130 This efficient method that combines qRT-PCR and high resolution melting (HRM) could be appli

131 Comparative qRT-PCR and ELISA showed that SCs expressed higher level

132 Confirmatory qRT-PCR analysis demonstrated good correlation with SnoR

133 ssion activation and chromatin conformation: qRT-PCR and mRNA in situ hybridization showed that the c

134 y improving nucleic acid recovery, designing qRT-PCR primers and a probe for a highly conserved regio

135 Our findings showed that the developed qRT-PCR could detect LGTV at a titre as low as 0.1 FFU/m

136 Here we describe a data-driven qRT-PCR normalization method, the minimum variance metho

137 Viral antigen-specific ELISAs, qRT-PCR and TCID50 infectious assays were utilized to de

138 (p=0.018 for RNA sequencing and p=0.0095 for qRT-PCR) and in the independent South African and Gambia

139 Data from qRT-PCR, patch clamp, ex vivo coronary perfusion Langend

140 Furthermore, qRT-PCR analysis of the AD postmortem brains with differ

141 Furthermore, qRT-PCR profiling of key ripening regulatory genes indic

142 Furthermore, qRT-PCR revealed up-regulation of miR-142-3p (6.4-fold)

143 Furthermore, qRT-PCR validations confirmed these co-increases in PD l

144 as approximately 70% accurate using in-house qRT-PCR influenza A as a gold-standard comparison.

145 However, qRT-PCR analysis of RNA extracted from 200 muL of serum

146 However, qRT-PCR analysis showed that endogenous miR-140/141/200c

147 However, qRT-PCR does not confirm presence of infectious virus, p

148 normal parathyroid by in situ hybridization, qRT-PCR, and immunohistochemistry.

149 elial cells (MeT5A) by immunohistochemistry, qRT-PCR and ELISA.

150 murine LG was examined using immunostaining, qRT-PCR, and RT(2)Profiler PCR microarrays.

151 In qRT-PCR validations, pRSEM was shown to be superior than

152 xpression at both mRNA and protein levels in qRT-PCR and Western blot analyses, respectively.

153 HKG stability in qRT-PCR was determined using geNorm, NormFinder, BestKee

154 RNA extracted from the neurons was used in qRT-PCR analysis.

155 ion, microarray experiments, and independent qRT-PCR validation analyses revealed that the branch rep

156 data demonstrate the utility of interpreting qRT-PCR results in the context of the course of EBOV inf

157 proaches to simulate results from microarray/qRT-PCR platforms and a local probabilistic model to ass

158 This microfluidic qRT-PCR system offers a practical approach to rapid anal

159 Advances in multiplex qRT-PCR have enabled increasingly accurate and robust qu

160 was introduced and detected in a multiplexed qRT-PCR format.

161 The results of qRT-PCR and western blot assay clearly showed that all o

162 nguishable when studied by flow cytometry or qRT-PCR.

163 receptors was evaluated by flow cytometry or qRT-PCR.

164 Our qRT-PCR and immunoblotting analysis revealed that reduce

165 Finally, our qRT-PCR-based approach may be broadly applicable to the

166 or selected human and mouse candidate pairs, qRT-PCR and in vitro RNA structure probing supported bot

167 Highly parallel qRT-PCR analysis of 334 single neurons selected by membe

168 Quantitative PCR (qRT-PCR) and Western blotting confirmed changes in expre

169 apidly replacing real-time quantitative PCR (qRT-PCR) as an efficient method of independent DNA quant

170 Real-time quantitative PCR (qRT-PCR) confirmed the results of light microscopy immun

171 ng SnoRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoRNA expression levels in muri

172 ing, reverse transcription-quantitative PCR (qRT-PCR), and carbohydrate utilization studies.

173 d by reverse transcription-quantitative PCR (qRT-PCR), was observed upon infection with strains from

174 vegf was determined by quantitative RT-PCR (qRT-PCR) and immunoblotting.

175 the multiplex real-time quantitative RT-PCR (qRT-PCR) assay for DENV-1, -3, and -4 detection but 10-f

176 was determined by using quantitative RT-PCR (qRT-PCR) in cell pellets.

177 om microarrays and from quantitative RT-PCR (qRT-PCR) measurements, based on the expression of a smal

178 modulated by steroids, quantitative RT-PCR (qRT-PCR) mRNA expression, enzymatic assay aromatase acti

179 cation (RT-LAMP) assay, quantitative RT-PCR (qRT-PCR), and IgM- and IgG-capture enzyme-linked immunos

180 reaction (RT-PCR), and quantitative RT-PCR (qRT-PCR), and the proinflammatory cytokines interleukin

181 splice-isoform-specific quantitative RT-PCR (qRT-PCR), nanoproteomics, stromal coculture, and BC LSC

182 We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in

183 oarray and confirmed by quantitative RT-PCR (qRT-PCR).

184 Quantitative real-time PCR (qRT-PCR) analysis of selected genes also validated the i

185 titative assays, quantitative real-time PCR (qRT-PCR) and droplet digital PCR (ddPCR), gave similar r

186 these findings, quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) a

187 Quantitative real-time PCR (qRT-PCR) and immunofluorescence were performed at PI-15

188 rthern blotting, quantitative real time PCR (qRT-PCR) and microarray technology besides novel techniq

189 A quantitative real-time PCR (qRT-PCR) assay with single-copy sensitivity targeting HI

190 vels analyzed by quantitative real time PCR (qRT-PCR) at different time points after harvest at 20 de

191 validation using quantitative real-time PCR (qRT-PCR) indicated four promising candidate genes having

192 s and subsequent quantitative real-time PCR (qRT-PCR) revealed that type 1 fimbria structural genes a

193 r-derived CTC by quantitative real-time PCR (qRT-PCR) to investigate transcriptional changes, enablin

194 ntified protein, quantitative real time PCR (qRT-PCR) was performed.

195 Using quantitative real-time PCR (qRT-PCR) we have measured the gene expression profiles o

196 Using quantitative real-time PCR (qRT-PCR), it showed that the DRELFA is very effective in

197 ion to multiplex quantitative real-time PCR (qRT-PCR), the signature was used to predict tuberculosis

198 ray analysis and quantitative real-time PCR (qRT-PCR).

199 validated using quantitative real-time PCR (qRT-PCR).

200 generates) using quantitative real-time PCR (qRT-PCR).

201 itative real-time reverse transcriptase PCR (qRT-PCR) analysis.

202 reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nucleic acid sequence-based am

203 ays, quantitative reverse transcriptase PCR (qRT-PCR), and transcriptional reporter fusions of select

204 sing quantitative reverse-transcriptase PCR (qRT-PCR), immunofluorescence, and Luminex technology.

205 and quantitative reverse transcriptase PCR (qRT-PCR).

206 itative real-time reverse-transcriptase-PCR (qRT-PCR) analyses revealed decreased expression of a num

207 ion, quantitative reverse transcriptase-PCR (qRT-PCR) and immunoblot experiments demonstrated direct

208 d by quantitative reverse-transcription PCR (qRT-PCR) and immunohistochemistry.

209 s of quantitative reverse-transcription PCR (qRT-PCR) demonstrated that expression of the genes encod

210 d by quantitative reverse-transcription PCR (qRT-PCR).

211 and quantitative reverse transcription-PCR (qRT-PCR) analyses confirm that HSV-1 latently infects ne

212 Quantitative reverse transcription-PCR (qRT-PCR) analyses revealed that both drugs altered E1A R

213 sing quantitative reverse transcription-PCR (qRT-PCR) and microarrays have shown a significant transi

214 Ups quantitative reverse transcription-PCR (qRT-PCR) and sequencing with degenerate DBL1alpha domain

215 step quantitative reverse transcription-PCR (qRT-PCR) assay for detection of OPV Sabin strains 1, 2,

216 time quantitative reverse transcription-PCR (qRT-PCR) assay using the recombinant thermostable Thermu

217 time quantitative reverse transcription-PCR (qRT-PCR) further demonstrated that SE-1 reduced the expr

218 ough quantitative reverse transcription-PCR (qRT-PCR) tests indicated early and persistent lung infec

219 sing quantitative reverse transcription-PCR (qRT-PCR) to further characterize expression of predicted

220 Quantitative reverse transcription-PCR (qRT-PCR) validated changes from the microarrays in known

221 n by quantitative reverse transcription-PCR (qRT-PCR), flow cytometry, and transwell migration experi

222 with quantitative reverse transcription-PCR (qRT-PCR), to augment or potentially replace the DFA test

223 d by quantitative reverse transcription-PCR (qRT-PCR).

224 d by quantitative reverse transcription-PCR (qRT-PCR).

225 time quantitative reverse transcription-PCR (qRT-PCR); these genes include chromosomal and plasmid-bo

226 gation was carried out combining proteomics, qRT-PCR mRNA transcripts analysis, and enzyme activities

227 analyzed by in-gel fluorescence, proteomics, qRT-PCR, immunofluorescence, fluorescence resonance ener

228 ires less hands-on time than other published qRT-PCR protocols for RVA detection.

229 of normal human hippocampus and quantitative qRT-PCR of human inducible pluripotent stem cells (iPSCs

230 rse transcription polymerase chain reaction (qRT-PCR) amplification of miRNA extracted directly from

231 rse transcription polymerase chain reaction (qRT-PCR) analysis.

232 itative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry, and found that DMT1 w

233 itative real-time polymerase chain reaction (qRT-PCR) and RNA in situ hybridization.

234 rse-transcription polymerase chain reaction (qRT-PCR) and western blotting and hepatic stellate cell

235 rse transcription polymerase chain reaction (qRT-PCR) array on 156 samples.

236 rse transcription-polymerase chain reaction (qRT-PCR) assay in support of pharmacokinetic and toxicok

237 rse transcription-polymerase chain reaction (qRT-PCR) could exceed 5 days.

238 rse transcription polymerase chain reaction (qRT-PCR) data shows the highest abundance of both groups

239 rse-transcription polymerase chain reaction (qRT-PCR) for expression of runt-related transcriptor fac

240 rse-transcription-polymerase chain reaction (qRT-PCR) for rapid detection of LGTV.

241 rse transcription-polymerase chain reaction (qRT-PCR) in biopsy samples of 19 patients with esophagea

242 rse transcriptase polymerase chain reaction (qRT-PCR) in colonic mucosal biopsies of patients from th

243 rse transcription polymerase chain reaction (qRT-PCR) measurements showed rapid initial RNA damage fo

244 rse transcription polymerase chain reaction (qRT-PCR) to compare the transcriptional profiles of whol

245 itative real time polymerase chain reaction (qRT-PCR) to further confirm the specificity of the scree

246 rse transcription polymerase chain reaction (qRT-PCR) was employed to track patterns in the expressio

247 rse transcription polymerase chain reaction (qRT-PCR) was performed in 2 groups of BE patients who ei

248 rse transcription-polymerase chain reaction (qRT-PCR) was performed to assess the messenger RNA (mRNA

249 rse transcriptase polymerase chain reaction (qRT-PCR) was used to confirm differential expression of

250 rse transcription-polymerase chain reaction (qRT-PCR), a sensitive method to detect viral genetic mat

251 rse transcriptase polymerase chain reaction (qRT-PCR)-based threshold cycle (Ct) value and the presen

252 rse transcription polymerase chain reaction (qRT-PCR).

253 rse transcription polymerase chain reaction (qRT-PCR).

254 itative real-time polymerase chain reaction (qRT-PCR).

255 rse transcription-polymerase chain reaction (qRT-PCR).

256 erse-transcription polymerase chain reaction(qRT-PCR) verification.

257 xpression in single samples using repetitive qRT-PCR assays.

258 l to establishing sensitive and reproducible qRT-PCR-based assays.

259 of guard cell aperture, we used large-scale qRT-PCR to compare circadian oscillator gene expression

260 Combined RNA sequencing, qRT-PCR, CLIP-ADAR1, and pri-let-7 mutagenesis data sugg

261 cation and re-sequencing, and have a similar qRT-PCR detection ratio as their cognate canonical miRNA

262 the ReEBOV RDT, compared with EBOV-specific qRT-PCR.

263 to conventional preparations and supporting qRT-PCR and RNA-Seq analyses.

264 0(-9) to 10(-15) M, and higher accuracy than qRT-PCR.

265 The qRT-PCR results showed that 11 (91.7%) of the 12 predict

266 The qRT-PCR results showed that 3 (75.0%) of the 4 predicted

267 good correlation between the DRELFA and the qRT-PCR over a 50-fold concentration range.

268 using a DENV NS1 antigen rapid test and the qRT-PCR.

269 Additionally, we compared the qRT-PCR assay to the gold standard direct fluorescent-an

270 The detection limit of the qRT-PCR assay at 95% probability was 0.28 FFU/ml as dete

271 Real-time qRT-PCR revealed that miR-125a-3p, miR-320c were signifi

272 isualization, Western blotting and real-time qRT-PCR were used to study and quantify GFP reporter pro

273 d assays, immunoblot analysis, and real-time qRT-PCR.

274 Here we describe a triplex qRT-PCR assay, including assembly and evaluation for sen

275 Importantly, unlike qRT-PCR, the microelectrochemical sensor offers direct a

276 We use qRT-PCR to validate differential expression and differen

277 We used qRT-PCR and genotyping to characterize residual circulat

278 Using a rat model, we used qRT-PCR to examine expression levels of inflammatory, cy

279 We used qRT-PCR, Western blotting, ELISA, and ChIP (chromatin im

280 marker genes (TNF-alpha, IL-8, IP-10) using qRT-PCR.

281 Samples were analyzed using qRT-PCR and metabolomics.

282 ilostazol and milrinone, then analyzed using qRT-PCR, immunoprecipitation/Western blot, immunofluores

283 expressed and secreted) were assessed using qRT-PCR.

284 and methylation changes were confirmed using qRT-PCR and qMSP methods.

285 ; a finding that was further confirmed using qRT-PCR.

286 IL-33, TSLP and GATA3 were determined using qRT-PCR.

287 ncorporating additional investigations using qRT-PCR, Western blotting, tissue microarrays (TMA), and

288 ing of expression was tested in islets using qRT-PCR and Western blotting.

289 idated differentially expressed miRNAs using qRT-PCR.

290 Molecular screening using qRT-PCR and Western blotting demonstrated that aldostero

291 Experimental validation using qRT-PCR confirmed a selected set of genes that are diffe

292 Key results were verified using qRT-PCR.

293 Evaluation of key neural markers via qRT-PCR demonstrated more profound differences in gene e

294 Increases were confirmed with qRT-PCR in five genes that code for proteins involved in

295 nine genes were independently confirmed with qRT-PCR.

296 UPR PCR-array analysis coupled with qRT-PCR identified and confirmed that four transcripts i

297 fitting algorithm to match model output with qRT-PCR mRNA expression data following exogenous auxin t

298 s in the same biopsy samples was tested with qRT-PCR.

299 asion and wound healing assays together with qRT-PCR determination of epithelial-to-mesenchymal trans

300 ples (46.9%) were classified as viremic with qRT-PCR.