ライフサイエンスコーパス: quantitative PCR

1 amplicon pyrosequencing and high-throughput quantitative PCR.

2 ears at six sites throughout the Delta using quantitative PCR.

3 ELISpot, Cyp27b1 expression was measured by quantitative PCR.

4 patients with grass pollen allergy by using quantitative PCR.

5 ter S1P exposure by using flow cytometry and quantitative PCR.

6 termined by ELISA, cytometric bead array, or quantitative PCR.

7 cell states, which we verify by single-cell quantitative PCR.

8 ation microarray, expression microarray, and quantitative PCR.

9 UBD, and ZIC2 were validated using real-time quantitative PCR.

10 (n) DNA and mtRNA to nRNA were analyzed via quantitative PCR.

11 successfully demonstrated in real-time like quantitative PCR.

12 d PAFR mRNA expression was assessed by using quantitative PCR.

13 by using next-generation DNA sequencing and quantitative PCR.

14 strained and relaxed substrates by Cas9 with quantitative PCR.

15 ell lysates using parallel DNA sequencing or quantitative PCR.

16 lymphoid cells (ILCs) were defined by using quantitative PCR.

17 (botulinum toxin A treated) attachments for quantitative PCR.

18 histology, immunohistochemistry, ELISA, and quantitative PCR.

19 assessed using a dog-specific microarray and quantitative PCR.

20 s, and bacterial numbers were assessed using quantitative PCR.

21 mal expression were validated with real-time quantitative PCR.

22 posttreatment PB samples were quantified by quantitative PCR.

23 The presence of these CNVs was confirmed by quantitative PCR.

24 s determined by reverse transcription-linked quantitative PCR.

25 ears 15, 25) were measured in whole blood by quantitative PCR.

26 detect the state of operational tolerance by quantitative PCR.

27 All patients were monitored by real-time quantitative PCR.

28 dated the presence of Helicobacter pylori by quantitative PCR.

29 levels in human macrophages, as detected by quantitative PCR.

30 (IDO) were analyzed using flow cytometry and quantitative PCR.

31 levels in primary HUVECs over 24 hours using quantitative PCR.

32 NA) in cerebrospinal fluid were amplified by quantitative PCR.

33 n the Huntington's gene of genomic DNA using quantitative PCR.

34 two distinct antibodies and MCPyV DNA using quantitative PCR.

35 tissue levels via time-lapse microscopy and quantitative PCR.

36 R1, and PTK2 were determined using real-time quantitative PCR.

37 or harboring HDV RNA detectable by real-time quantitative PCR.

38 was measured by using standardized real-time quantitative PCR.

39 analyzed by 16S rRNA amplicon sequencing and quantitative PCR.

40 human beta-cells was performed by real-time quantitative PCR.

41 Leptin receptor (Ob-R) was evaluated by quantitative PCR.

42 ld more than the other isoforms by real-time quantitative PCR.

43 /mL or IU/mL, respectively, as determined by quantitative PCR.

44 g, respectively) was quantified by real-time quantitative PCR.

45 was confirmed by RNA immunoprecipitation and quantitative-PCR.

46 Quantitative PCR analyses showed an increase in Hcrtr2 m

47 Combining quantitative PCR analyses with immunofluorescence studie

48 ion profiles were compared by microarray and quantitative PCR analyses.

49 cytokine expression pattern was confirmed by quantitative PCR analysis and ELISA.

50 Using RT-quantitative PCR analysis and gene promoter::GUS fusions

51 Quantitative PCR analysis and GUS activity measurements

52 smic and nuclear accumulation, and data from quantitative PCR analysis closely recapitulated the EdU

53 ofiles using multiple V regions validated by quantitative PCR analysis confirmed that distinct bacter

54 Quantitative PCR analysis of different rat arteries foun

55 Western blotting and quantitative PCR analysis of infected cells treated with

56 By microarray and quantitative PCR analysis, we identified trpa1 as an L c

57 essed by staining, gene expression, and ChIP-quantitative PCR analysis.

58 assessed by histologic, flow cytometric, and quantitative PCR analysis.

59 Here, we combined metatranscriptomics, quantitative PCR and 16S rRNA gene sequencing to study t

60 t baseline and after 4 weeks and analyzed by quantitative PCR and 16S rRNA sequencing.

61 Here, we used quantitative PCR and also analyzed reporter gene express

62 mtDNA damage was measured by using quantitative PCR and apoptosis via ELISA.

63 marker genes after RKN infection using both quantitative PCR and beta-glucuronidase reporter transge

64 on the coral Montastraea cavernosa, and used quantitative PCR and chlorophyll fluorometry to assess t

65 the number of CFHR3 and CFHR1 gene copies by quantitative PCR and collected clinical and biologic dat

66 merase chain reaction [PCR]) and BK viruria (quantitative PCR and decoy cell counts).

67 We utilized quantitative PCR and double immunofluorescence microscop

68 Quantitative PCR and ELISA were performed to quantify Il

69 d selected mediators were evaluated by using quantitative PCR and ELISA.

70 cytokine and chemokine levels by performing quantitative PCR and ELISA.

71 upregulated or downregulated using real-time quantitative PCR and found a strong correlation between

72 We also showed by robust RT-quantitative PCR and genetic interaction analysis that t

73 , diversity and composition were revealed by quantitative PCR and high-throughput sequencing.

74 expression was evaluated by using real-time quantitative PCR and immunofluorescence.

75 Quantitative PCR and immunohistochemistry indicated post

76 Quantitative PCR and immunohistochemistry showed that HO

77 In the present study, we used quantitative PCR and immunohistochemistry to determine a

78 Senescence and fibrosis were measured by quantitative PCR and immunohistochemistry.

79 A fluorescence in situ hybridization (FISH), quantitative PCR and immunostaining.

80 BDNF mRNA levels, assessed by quantitative PCR and in situ hybridization at 1, 4, 7, a

81 RT-quantitative PCR and luciferase assays indicated that th

82 LTL was assessed using quantitative PCR and measured at baseline and after 6 ye

83 ion of HBoV1 serology and nasopharyngeal DNA quantitative PCR and mRNA RT-PCR should be used for accu

84 Quantitative PCR and multiplex were used to determine tr

85 Quantitative PCR and Nav1.8-immunoreactivity confirmed N

86 Quantitative PCR and NGS revealed the activation of key

87 Quantitative PCR and protein half-life experiments revea

88 h depths of various marine sediment cores by quantitative PCR and pyrosequencing using newly designed

89 b, was validated using reverse transcription quantitative PCR and receiver operating characteristic (

90 Transcription profiling by quantitative PCR and RNA-Seq suggested that disruption o

91 s correlated with viral burden determined by quantitative PCR and showed high intrarun and interrun r

92 pression and activity were assessed by using quantitative PCR and the telomere repeat amplification p

93 Quantitative PCR and western blot analysis confirmed los

94 I mRNA and protein levels were determined by quantitative PCR and Western blot analysis, respectively

95 of differentiation markers was evaluated by quantitative PCR and Western blot.

96 n immunoprecipitation, reverse transcriptase quantitative PCR and western blotting analyses, we confi

97 dermal keratinocytes (n = 11) with real-time quantitative PCR and Western blotting revealed up-regula

98 sociation with gene expression using in vivo quantitative PCR and with affective behavior using a pri

99 Quantitative PCR and/or ELISA validated that growth fact

100 We used quantitative PCRs and a telomere-sequence to single-copy

101 shmania donovani, spatial analyses at macro-(quantitative PCR) and micro-(confocal microscopy) scales

102 -stimulated gene expression were measured by quantitative PCR, and changes in cytokine production wer

103 etry, Toll-like receptor (TLR) expression by quantitative PCR, and cytokine secretion after stimulati

104 in a four-stage study including microarray, quantitative PCR, and flow cytometry analyses.

105 e filtration was evaluated by counting, CD45 quantitative PCR, and HIV-1 DNA quantification.

106 by means of telemetry, Il33 mRNA by means of quantitative PCR, and IL-33, OVA-specific IgE, and mouse

107 m patients with EoE in immunohistochemistry, quantitative PCR, and immunoassay experiments to underst

108 an brain tissue samples by Western blotting, quantitative PCR, and immunohistochemistry.

109 yzed by microarray and reverse transcription quantitative PCR, and linked with function by further pr

110 eic acid were observed in aborted fetuses by quantitative PCR, and PCV3 antigen was localized in hist

111 ls segregating for different spot positions, quantitative PCR, and pyrosequencing, were used to confi

112 in thymocytes by protein immunoblotting and quantitative PCR, and show that expression is confined t

113 Our in situ hybridization, real-time quantitative PCR, and stage-specific transcriptomic anal

114 Microarray, quantitative PCR, and Western blot analyses revealed tha

115 Quantitative PCR array analyses identified multiple cell

116 Gene expressions were analyzed by quantitative PCR array and Western blot.

117 We measured 754 plasma miRNAs by quantitative PCR array in a discovery cohort of 22 indiv

118 s assessed by means of quantitative PCR or a quantitative PCR array in vehicle- or IL-13-treated BECs

119 We found by growth factor quantitative PCR array that acidosis increases expressio

120 By using laser capture microdissection and a quantitative PCR array, 23 of 84 examined ISGs were foun

121 ted PCR targeting the cytochrome b gene) and quantitative PCR as reference standards.

122 erum tryptase determination, allele-specific quantitative PCR (ASqPCR) for the KIT D816V mutation, an

123 Through use of a quantitative PCR assay for HHpgV-1, infection was also d

124 Factor XIII-A recombination was evaluated by quantitative PCR assay of genomic DNA from liver and spl

125 Furthermore, chromatin immunoprecipitation-quantitative PCR assay showed enrichment of p63 on Sun1,

126 ed for HIV shedding and VL using a real-time quantitative PCR assay.

127 Using quantitative PCR assays and a fluorescence miRNA sensor,

128 , using Ventana in situ hybridisation (ISH), quantitative PCR assays and biomarkers of productive and

129 We developed novel quantitative PCR assays for cell-associated (CA) HIV-1 D

130 icipating in proficiency testing surveys for quantitative PCR assays of cytomegalovirus (CMV), Epstei

131 In contrast to conventional quantitative PCR assays or decoy cell counts, quantitati

132 Quantitative PCR assays revealed that HNF4A and PTBP1 mR

133 Epstein-Bar virus (EBV) load testing in four quantitative PCR assays, treating digital PCR as a refer

134 Quantitative PCR assessment of blaOXA and blaGES genes i

135 We used a quantitative PCR-based assay to determine fragment size

136 We developed a quantitative PCR-based lymph node infiltration assay to

137 clinical cohort of HPV(+/-) OPSCC tumors by quantitative PCR-based miRNA profiling.

138 with that from non-ALS patients was found by quantitative PCR, but no differential expression was fou

139 (ChIP-seq) and chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) indicate that in LCLs inhib

140 cular and histochemical approaches including quantitative PCR, chromatin immunoprecipitation, and 3,3

141 Quantitative PCR confirmed this classification and showe

142 Collection on filter paper followed by quantitative PCR could be useful for density measurement

143 Real-time quantitative PCR detected mRNAs for CD8(+) T-cell activa

144 In contrast, quantitative PCR detected the presence of the gene for i

145 f R. felis in mosquitoes was demonstrated by quantitative PCR detection of the bacteria up to day 15

146 sults, we assessed the transcript levels (by quantitative PCR), DNA methylation status (by bisulfite

147 we demonstrate an electrochemical real-time quantitative PCR (e-PCR) measurement in the total DNA ex

148 y, cocultures, suppression assays, real-time quantitative PCR, ELISAs, and ELISpot assays were perfor

149 were subjected to mRNA extraction, real-time quantitative PCR, enzyme-linked immunosorbent assay, and

150 hese iNs, we performed electrophysiology and quantitative PCR experiments that demonstrated increased

151 as evaluated using lake water microcosms and quantitative PCR for a Common Carp-specific genetic mark

152 Quantitative PCR for BCR-ABL1 yielded a value of 29.6% o

153 oved agreement among laboratories performing quantitative PCR for CMV.

154 We used bead-based multiplex assays and quantitative PCR for cytokine quantification.

155 rom BLT1(+/+) and/or BLT1(-/-) mice and used quantitative PCR for gene expression analysis in termina

156 Newer methods have proliferated and include quantitative PCR for organism load, AmpliSens PCR, PCR f

157 Intestinal inflammation was assessed by quantitative PCR for proinflammatory markers and colon l

158 mariner, and TE accumulation was tracked by quantitative PCR for up to 100 generations.

159 Quantitative PCR gene expression studies of glycosyltran

160 Quantitative PCR highlighted significant changes in expr

161 cyte-derived macrophages was investigated by quantitative PCR, immunoblotting and flow cytometry.

162 d in different malt and feed varieties using quantitative PCR, immunoblotting, and enzyme-linked immu

163 lasma miRNAs was measured by using real-time quantitative PCR in 35 asthmatic patients, 25 nonasthmat

164 sessed by using a plaque reduction assay and quantitative PCR in a susceptible mammalian epithelial c

165 d Cacna1c expression levels were examined by quantitative PCR in fluorescence-activated cell sorting.

166 ngesting modified LDL; this was validated by quantitative PCR in human and murine macrophages.

167 -34 mRNA levels were quantified by real-time quantitative PCR in human synovial fibroblasts and murin

168 2s and DCreg cells were assessed by means of quantitative PCR in PBMCs from 80 patients with grass po

169 and let-7f expression was measured by using quantitative PCR in TH17-differentiated cells from healt

170 /chemokines and the levels of parasitemia by quantitative PCR in the circulation of neonates born to

171 d XDH1 and XDH2 gene expression by real-time quantitative PCR in tissues from sugar- and blood-fed fe

172 Analysis of gene expression by real time quantitative PCR indicated that whitefly mortality was a

173 Cytokine levels (ELISA and quantitative PCR), inflammatory cell profile, and AHR (f

174 a single FRET experiment using conventional quantitative PCR instrumentation, 19,400 conditions of M

175 Quantitative PCR is a diagnostic mainstay of clinical vi

176 Telomere length was assessed using the quantitative PCR method of telomere length relative to s

177 either directly, using reverse transcription-quantitative PCR, microarrays, and flow cytometry, or in

178 Using high-throughput quantitative PCR nanofluidics arrays, the expression of

179 several viral mRNAs by reverse transcriptase-quantitative PCR, no production of infectious virus, and

180 tion of high-throughput deep RNA sequencing, quantitative PCR, Northern blotting, and rapid amplifica

181 fluenced by human age and skin physiology by quantitative PCR of 51 different skin samples taken from

182 Quantitative PCR of ca19 and ca18 and protein expression

183 16S rRNA gene sequencing and quantitative PCR of cecal and fecal samples demonstrated

184 Quantitative PCR of mRNA from biofilms from a 24-h conti

185 Quantitative PCR of stool DNA with species-specific prim

186 s were independently validated by ChIP-qPCR (quantitative PCR), oligonucleotide-binding assays and an

187 This was followed by quantitative PCR on pools and individual lines of the Po

188 -5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of patients with

189 documented PG-G hydrolases in neutrophils by quantitative PCR, only ABHD12 and ABHD16A were detected

190 hemokine expression was assessed by means of quantitative PCR or a quantitative PCR array in vehicle-

191 bsence of certain antibiotics with real-time quantitative PCR or digital PCR to determine antimicrobi

192 sing Western blot, flow cytometry, real-time quantitative PCR, or RNA sequencing analyses.

193 ed in catalase-induced MDSC as determined by quantitative PCR outlining the importance of oxidative b

194 han did endoscopic brushes or biopsies using quantitative PCR (p<0.0001).

195 trategy that combines proportion competitive quantitative PCR (PCQ-PCR) and lateral flow nucleic acid

196 fferential gene expression using custom-made quantitative PCR plates.

197 s study, we aimed to establish a single-cell quantitative PCR platform and perform side-by-side compa

198 we applied a recently established real-time quantitative PCR platform to gain insight into transcrip

199 ration RNA sequencing of mRNAs and real time quantitative PCR profiling established that Ezh2 inactiv

200 Using quantitative PCR (q-PCR), immunostaining and patch clamp

201 -C mRNA expression levels were determined by quantitative PCR (qPCR) (p = 1.8 x 10(-20)) and in two c

202 Quantitative PCR (qPCR) analysis confirmed the deletion,

203 a2, and chromatin immunoprecipitation (ChIP)-quantitative PCR (qPCR) analysis of their promoters reve

204 Quantitative PCR (qPCR) analysis of WGA products was use

205 n downstream processing applications such as quantitative PCR (qPCR) and high-throughput RNA sequenci

206 minutum of the Mediterranean Sea using both quantitative PCR (qPCR) and HILIC-HRMS techniques.

207 tient over a period of 3 years and performed quantitative PCR (qPCR) and Illumina sequencing on those

208 cision in comparison with techniques such as quantitative PCR (qPCR) and melt curve analysis.

209 Here, ddPCR was compared to quantitative PCR (qPCR) and pyrosequencing.

210 and caspase assays; and gene expression with quantitative PCR (qPCR) and RNA sequencing on lung epith

211 Here we describe a novel real-time quantitative PCR (qPCR) approach based on the SYBR green

212 ion was compared to that with a conventional quantitative PCR (qPCR) assay and blood culture.

213 of the Xpert Carba-R test, a rapid real-time quantitative PCR (qPCR) assay that detects five families

214 Quantitative PCR (qPCR) assays were used to determine th

215 tionship between culturable counts (CFU) and quantitative PCR (qPCR) cell equivalent counts of Escher

216 Using quantitative PCR (qPCR) for common respiratory viruses a

217 RNA gene and polymerase chain reaction (PCR)/quantitative PCR (qPCR) for Streptococcus mutans and Str

218 ween 40 and 69 years of age were analyzed by quantitative PCR (qPCR) for the presence of iciHHV-6.

219 cy between PCR from a diagnostic laboratory, quantitative PCR (qPCR) from a research laboratory, 16S

220 Quantitative PCR (qPCR) has become the gold standard tec

221 Real-time quantitative PCR (qPCR) is one of the most powerful tech

222 dPCR quantification was also consistent with quantitative PCR (qPCR) measurements as well as cell cou

223 s challenging to quantify reproducibly using quantitative PCR (qPCR) or next generation sequencing (N

224 16S sequencing/quantitative PCR (qPCR) revealed significant changes in

225 In experiment 1, quantitative PCR (qPCR) was performed at several stages

226 expression, including hybridization arrays, quantitative PCR (qPCR), and high-throughput sequencing.

227 men were tested by microscopy (micromethod), quantitative PCR (qPCR), and immunoglobulin (Ig)M trypom

228 Microarray, quantitative PCR (qPCR), and protein assays performed on

229 Using quantitative PCR (qPCR), droplet digital PCR, and fluore

230 odontal pathogens using microarray analysis, quantitative PCR (qPCR), enzyme-linked immunosorbent ass

231 Its advantages over quantitative PCR (qPCR), including absolute quantificati

232 Using quantitative PCR (qPCR), we demonstrate the upregulation

233 entified 45 of 48 that were PCV3 positive by quantitative PCR (qPCR), with 60% of a subset also testi

234 y for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the eff

235 productively infected with VZV using TaqMan quantitative PCR (qPCR).

236 combination of 16S rRNA gene sequencing and quantitative PCR (qPCR).

237 hybridized to a miRNA followed by real-time quantitative PCR (qPCR).

238 rmed histological assays, Western blots, and quantitative PCR (qPCR).

239 nase (amoA) abundance quantification through quantitative PCR (qPCR).

240 tly detected in the plasma of patients using quantitative PCR (qPCR).

241 2,700 patient samples for EBOV detection by quantitative PCR (qPCR).

242 ng a systematic bioinformatics approach with quantitative-PCR (qPCR) validation, we show that only BL

243 nely applied after culture (spoligotyping or quantitative PCR [qPCR]) in each laboratory; liquid (MGI

244 Quantitative PCR (qRT-PCR) and Western blotting confirme

245 Using SnoRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoRNA express

246 ole-genome sequencing, reverse transcription-quantitative PCR (qRT-PCR), and carbohydrate utilization

247 after the 12-week treatment using real-time quantitative PCR (RT-qPCR) analysis.

248 n, including real-time reverse transcription-quantitative PCR (RT-qPCR) and commercially available im

249 reaction kinetics with reverse transcription quantitative PCR (RT-qPCR) and quantitative matrix-assis

250 ares the accuracies of reverse transcription-quantitative PCR (RT-qPCR) and reverse transcription-dig

251 A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades prob

252 and were addressed by reverse transcription-quantitative PCR (RT-qPCR) of the viruses involved.

253 specimens collected by reverse transcriptase quantitative PCR (RT-qPCR) targeting a conserved region

254 ng, and site-directed mutagenesis, real-time quantitative PCR (RT-qPCR), and Western blotting analysi

255 mation, mainly through reverse transcription-quantitative PCR (RT-qPCR), is a common approach employe

256 conventional viral RNA reverse transcription-quantitative PCR (RT-qPCR)-based assays and from outgrow

257 confirmed by real-time reverse transcription-quantitative PCR (RT-qPCR).

258 (DD) as determined by reverse transcription-quantitative PCR (RT-qPCR).

259 vels are determined by reverse-transcription quantitative PCR (RT-qPCR).

260 shedding of Sabin 2 by reverse transcriptase quantitative PCR (RT-qPCR).

261 for genotype A measles virus (MeV) (MeVA RT-quantitative PCR [RT-qPCR]) that can identify measles va

262 we developed and performed a high-throughput quantitative PCR screen complemented by a novel orthogon

263 By combining targeted quantitative PCR screens with bioinformatic analysis and

264 Quantitative PCR showed a positive correlation between t

265 Quantitative PCR showed that the mRNA levels of chemokin

266 Quantitative PCR showed that the relative expression of

267 wide analysis and subsequent validation with quantitative PCR supported that endogenous expression of

268 We determined parasite loads with the use of quantitative PCR testing and assessed infectivity by mea

269 g of latrines and detection of M. bovis with quantitative PCR tests, the results of which strongly co

270 negative neurons in dorsal striatum and used quantitative PCR to assess gene expression within these

271 We used quantitative PCR to test for cytomegalovirus, BK polyoma

272 criptome profiling by microarray followed by quantitative PCR validation.

273 However, we carried out quantitative PCR validations which indicate that microar

274 Real Time-quantitative PCR was performed for SERPINA3 transcript,

275 n was measured by using ELISA, and real-time quantitative PCR was performed to detect PGE2 receptor e

276 quantitative PCR was performed to determine factor XIII-

277 y enzyme-linked immunosorbent assay, whereas quantitative PCR was performed to measure interleukin-6

278 n sequencing of the 16S rRNA gene as well as quantitative PCR was performed.

279 Quantitative PCR was used to compare bacterial load and

280 Quantitative PCR was used to estimate the abundances of

281 Quantitative PCR was used to measure mRNA for sHBEC thym

282 Flow cytometry and quantitative PCR was used to measure type 2 cytokines.

283 Real-time quantitative PCR was used to quantitate LYN, SYK, and CD

284 Using RT(2) Profiler PCR Array and real-time quantitative PCR we found several important synaptic pla

285 By microarray analysis and quantitative PCR we identified and validated FBXO11 (a m

286 Moreover, using quantitative PCR, we also find that adult male S. parvus

287 Using 16S rRNA gene sequencing and quantitative PCR, we characterized the gut microbiomes o

288 Using quantitative PCR, we demonstrated that the ORF25 deleted

289 Using quantitative PCR, we examined their transcript levels at

290 Upon validation with quantitative PCR, we studied the association of the top

291 ption at low dNTP concentrations followed by quantitative-PCR, we found that the same adenosine resid

292 Microtomography, histology, and real-time quantitative PCR were performed to analyze bone paramete

293 ter fusions to gfp and reverse transcription-quantitative PCR, were distinct from that of hetP in bot

294 topathological assessment, saliva flow rate, quantitative PCR, Western blot analyses and immunofluore

295 from our own molecular genetic experiments (quantitative PCR, Western blot, EMSA) or genome-wide ass

296 hronic rhinosinusitis were analyzed by using quantitative PCR, Western blotting, and immunohistochemi

297 These results were confirmed by quantitative PCR, which revealed significant enrichment

298 cytometry, immunohistochemistry, ELISA, and quantitative PCR with knockout and reporter mice to diss

299 Using a targeted quantitative PCR with reverse transcription approach in

300 Quantitative PCR yielded no Escherichia coli and few ins