ライフサイエンスコーパス: quantitative real-time PCR

1 nce it was first described in the mid-1990s, quantitative real time PCR (Q-PCR) has been widely used

2 A quantitative real-time PCR (qRT-PCR) assay with single-c

3 A quantitative real-time PCR (RT-PCR) method, employing no

4 Additionally, a quantitative real-time PCR (qPCR) was performed to estim

5 Additionally, a quantitative real-time PCR approach was proposed, allowi

6 Employing a quantitative real-time PCR assay, we determined the repl

7 In this study, a quantitative real-time PCR (qRT-PCR) was used to examine

8 In addition, by using a quantitative real-time PCR-based viral array, we are the

9 e gene expression analysis and would achieve quantitative real-time PCR replication pre-ITx (another

10 In addition, quantitative real-time PCR revealed that intracellular a

11 Additionally, quantitative real-time PCR studies showed that the trans

12 oarrays together with binding site analysis, quantitative real-time PCR, chromatin immunoprecipitatio

13 We analyzed quantitative real-time PCR (qPCR) assays on DNA extracte

14 a chromosome conformation capture assay and quantitative real time PCR, we demonstrate that a chroma

15 Cs), we have used both miRNA microarrays and quantitative real time PCR to profile miRNA expression i

16 chemical in situ hybridization histology and quantitative real time-PCR studies.

17 ods of 4 days during the summer of 2012, and quantitative real-time PCR (qPCR) was used to enumerate

18 ombination of oligonucleotide-based aCGH and quantitative real-time PCR is an effective method of ide

19 red and subjected to microarray analysis and quantitative real-time PCR (qRT-PCR).

20 Here, microarray analysis and quantitative real-time PCR showed that miR-365 was robus

21 termined by genechip microarray analysis and quantitative real-time PCR, was compared to the young ad

22 chain reaction (PCR), sequence analysis, and quantitative real-time PCR.

23 Cell viability assays and quantitative real-time PCR showed that the peptide inter

24 Biochemical assays and quantitative real-time PCR were used to measure triglyce

25 amic areas were analysed by Western blot and quantitative real-time PCR (qRT-PCR) for expression of a

26 Using western blot and quantitative real-time PCR analysis, our results indicat

27 d to quantify the biomass in flow cells, and quantitative real-time PCR with species-specific primers

28 cted genes was confirmed with gene chips and quantitative real-time PCR techniques.

29 ccolithovirus strains, by flow cytometry and quantitative real-time PCR allowed tentative links to th

30 ction were examined using flow cytometry and quantitative real-time PCR.

31 gated using plate counts, flow cytometry and quantitative real-time PCR.

32 that enabled efficient uptake of decoys, and quantitative real-time PCR demonstrated decoy persistenc

33 t analysis, trypsin and DNase digestion, and quantitative real-time PCR.

34 d gene expression were assessed by ELISA and quantitative real-time PCR.

35 fluorescence, enzymatic assays (ELISA), and quantitative real-time PCR.

36 s as shown by both in situ hybridization and quantitative real-time PCR.

37 ed and analyzed for C5L2 and C5aR by IHC and quantitative real-time PCR.

38 rmed in cultured cells by immunoblotting and quantitative real-time PCR and in mouse kidneys by immun

39 17 signature by using immunofluorescence and quantitative real-time PCR.

40 3) was monitored by immunohistochemistry and quantitative real-time PCR (qRT-PCR).

41 ors was assessed by immunohistochemistry and quantitative real-time PCR in isolated islets and adenom

42 We used immunohistochemistry and quantitative real-time PCR to determine IL-31 expression

43 Using immunohistochemistry and quantitative real-time PCR, we assessed biopsy specimens

44 Using whole-genome microarray and quantitative real-time PCR analysis of endobronchial bio

45 Human cytokine microarray and quantitative real-time PCR results indicated that CS1 ac

46 ssion was measured by using a microarray and quantitative real-time PCR.

47 ive donor samples by in-house nested PCR and quantitative real-time PCR assays, respectively.

48 were validated by ChIP-quantitative PCR and quantitative real-time PCR.

49 ultiplex polymerase chain reaction (PCR) and quantitative real-time PCR.

50 red their mature, phasic firing property and quantitative real-time PCR confirmed a coincident increa

51 measured by solid phase radioimmunoassay and quantitative real-time PCR.

52 Microarray analysis revealed and quantitative real-time PCR (qRT-PCR) confirmed that grow

53 E, and phnAB occurs in these revertants, and quantitative real-time PCR experiments suggested that th

54 RNA sequencing and quantitative real-time PCR analyses revealed that, altho

55 High-throughput sequencing and quantitative real-time PCR showed a significant composit

56 Here, we used next-generation sequencing and quantitative real-time PCR to determine the impact of dr

57 r tissue MMP9 as evidenced by zymography and quantitative real-time PCR.

58 is suppressed were assayed by microarray and quantitative real-time-PCR analyses.

59 Microarray and quantitative real-time-PCR analysis of gonads showed ele

60 Furthermore, the LC-MS/MS and quantitative real-time-PCR analysis followed by inhibito

61 We also apply quantitative real-time PCR to several highly ranked non-

62 Microarray as well as quantitative real-time PCR analysis of p85alpha(-/-) ost

63 rgets were assessed using luciferase assays, quantitative real-time PCR and western blots in vitro an

64 ivity were assessed using luciferase assays, quantitative real-time PCR, western blots, scratch assay

65 Two quantitative assays, quantitative real-time PCR (qRT-PCR) and droplet digital

66 vivo confocal microscopy (IVCM) assessment, quantitative real-time PCR gene expression, C. trachomat

67 now developed a rapid peripheral blood-based quantitative real-time PCR assay that enables accurate p

68 This anti-primer-based quantitative real-time PCR (aQRT-PCR) allows simplex or

69 ction of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR) and microarray tech

70 Both quantitative real-time PCR (qRT-PCR) and Western blottin

71 By quantitative real time PCR, the level of kindlin-3 in en

72 duce and their expression levels analyzed by quantitative real time PCR (qRT-PCR) at different time p

73 Fibrotic response was assessed by quantitative real time PCR (qRT-PCR) of fibrotic marker

74 at mRNA and protein levels was confirmed by quantitative real time PCR (qRT-PCR) and immunocytochemi

75 cle actin (alphaSMA) and Smad7 expression by quantitative real time PCR.

76 on of immunocompetent mice, as determined by quantitative real time-PCR of ear tissue, by enzyme-link

77 By quantitative real-time PCR, we determined the mRNA copy

78 investigated in three sfr6 mutant alleles by quantitative real-time PCR and susceptibility to UV-C ir

79 MiR-125b expression was analyzed by quantitative real-time PCR and in situ hybridization in

80 , four were selected and further analyzed by quantitative real-time PCR at the transcriptome level to

81 ioid receptor mRNA expression as analyzed by quantitative real-time PCR in both male and female mice

82 a-submerged polystyrene pegs was analyzed by quantitative real-time PCR using species-specific primer

83 monitored by immunohistofluorescence and by quantitative real-time PCR measurement of Foxp3 mRNA.

84 cence assay to monitor NS1 expression and by quantitative real-time PCR.

85 enotyping accuracy of 99%, as ascertained by quantitative real-time PCR.

86 ession of Stat5 target genes were assayed by quantitative real-time PCR assay.

87 Gene expression was assessed by quantitative real-time PCR (QPCR) in one center.

88 Expression of cytokines was assessed by quantitative real-time PCR, and mast cells were visualiz

89 xpression, and localization were assessed by quantitative real-time PCR, Western blot analysis, and i

90 PEX material and TGF-beta1 were assessed by quantitative real-time PCR, Western blotting, immunohist

91 IFN-gamma, PD-1, and PD-L1 were assessed by quantitative real-time PCR.

92 y IHC, and immune mediators were assessed by quantitative real-time PCR.

93 tary zinc depletion were further assessed by quantitative real-time PCR.

94 sured in lysates from bronchial brushings by quantitative real-time PCR and Western blot.

95 We determined the KIT D816V allele burden by quantitative real-time PCR in bone marrow and peripheral

96 for the expression of relevant chemokines by quantitative real-time PCR and immunohistochemistry.

97 rimary murine NK cells, with confirmation by quantitative real-time PCR (qRT-PCR) and microarrays.

98 in the venom gland and this was confirmed by quantitative real-time PCR (qPCR) analysis, suggesting t

99 remaining 44 changes (45%) were confirmed by quantitative real-time PCR or standard cytogenetic techn

100 fear conditioning, miR-182, was confirmed by quantitative real-time PCR.

101 Gene expression was confirmed by quantitative real-time PCR.

102 icroRNA-21 (miR-21) was further confirmed by quantitative real-time PCR.

103 selected Notch glycogenes were confirmed by quantitative real-time PCR.

104 richment of several regions was confirmed by quantitative real-time PCR.

105 ly selected colorectal cancer-derived CTC by quantitative real-time PCR (qRT-PCR) to investigate tran

106 isk (LR) forms of PCa, was not detectable by quantitative real-time PCR in samples from healthy volun

107 se of differentiating cells were detected by quantitative real-time PCR.

108 eukin-22 (IL-22), and IL-17 were detected by quantitative real-time PCR.

109 CFU determination by quantitative real-time PCR (qPCR), however, is becoming

110 As determined by quantitative real-time PCR analysis, we found that level

111 smids is 23 in M. smegmatis as determined by quantitative real-time PCR and accounts for the previous

112 Renal Klotho expression was determined by quantitative real-time PCR and immunoblot analysis.

113 Gene expression was determined by quantitative real-time PCR and protein expression by Wes

114 and protein levels of CLN3 as determined by quantitative real-time PCR and Western blotting.

115 MP9 expression and activity as determined by quantitative real-time PCR and zymography.

116 a/b/c, miR-155, and let-7a was determined by quantitative real-time PCR in formalin-fixed paraffin-em

117 ulatory and fibrosis genes was determined by quantitative real-time PCR in livers and hearts of 52-we

118 periodontopathic bacteria were determined by quantitative real-time PCR.

119 In addition, we established by quantitative real-time PCR (qRT-PCR) that both fresh col

120 elevated more than 100-fold as estimated by quantitative real-time PCR in most MPNST cell lines.

121 Adiponectin mRNA levels were evaluated by quantitative real-time PCR in the visceral adipose tissu

122 Changes in gene expression were evaluated by quantitative real-time PCR.

123 d gene expression profiles were evaluated by quantitative real-time PCR.

124 esenchymal marker expression was examined by quantitative real-time PCR, Western blotting, and immuno

125 her confirmed in a subset of target genes by quantitative real-time PCR and beta-galactosidase transc

126 We have identified, by quantitative real-time PCR, hundreds of miRNAs that are

127 pendent cohort of patient-matched lesions by quantitative real-time PCR.

128 s of cuticle-associated gene mRNA levels, by quantitative real-time PCR, indicated a relationship bet

129 ns with different median strain lifespans by quantitative real-time PCR.

130 the symptomatic phase of ECM, as measured by quantitative real-time PCR analysis.

131 Beta-cell gene profiles were measured by quantitative real-time PCR and the effects on intracellu

132 and protein by more than 50% as measured by quantitative real-time PCR and Western blot analysis.

133 RNA) and protein expression were measured by quantitative real-time PCR and Western blot, respectivel

134 n of miRNA and target genes were measured by quantitative real-time PCR and/or Western blotting.

135 Measured by quantitative real-time PCR, capn1 transcript levels were

136 ssion of gluconeogenic genes was measured by quantitative real-time PCR.

137 for 45 immune-related genes was measured by quantitative real-time PCR.

138 gene expression changes in VMAT2hypo mice by quantitative real-time PCR and in situ hybridisation.

139 gelatin zymography, expression of MT1-MMP by quantitative real-time PCR analysis, levels of tissue in

140 We measured levels of microRNAs and mRNAs by quantitative real-time PCR analysis of RNA extracted fro

141 erfusion-induced microglial ISG responses by quantitative real-time PCR and demonstrated that both we

142 normal and malignant endometrial samples by quantitative real-time PCR and Northern blot analysis re

143 for bisulfide detection in bulk solution by quantitative real-time PCR, which achieved a linear work

144 ond larger cohort of ESCC tumor specimens by quantitative real-time PCR and immunohistochemical analy

145 localization of Populus PHYs was studied by quantitative real-time PCR (qRT-PCR) and protoplast tran

146 oropharyngeal swab specimens were tested by quantitative real-time PCR (qPCR) for feline herpesvirus

147 fied IL-10-inhibited genes also validated by quantitative real-time PCR (qRT-PCR) were Toll-like rece

148 I were identified in silico and validated by quantitative real-time PCR (qRT-PCR), Western blot analy

149 NA microarrays and were further validated by quantitative real-time PCR analysis.

150 ct patterns of gene expression, validated by quantitative real-time PCR and Western blot analysis, we

151 RNA-Seq data was validated by quantitative real-time PCR of selected differentially ex

152 expression analysis, which was validated by quantitative real-time PCR on a subset of genes, showed

153 We validated these results by quantitative real-time-PCR using NBUVB-treated vitiligo

154 nents of a dysbindin interaction network by, quantitative real time PCR and quantitative immunohistoc

155 Single-cell quantitative real-time PCR (qRT-PCR) combined with high-

156 They conducted quantitative real-time PCR on genomic DNA isolated from

157 filtrated pancreatic islets and confirmatory quantitative real time PCR and protein analyses identifi

158 We developed quantitative real-time PCR (qPCR) assays to facilitate a

159 Here we employed quantitative real-time PCR (qPCR) assays for polyphospha

160 We evaluated quantitative real-time PCR to establish the diagnosis of

161 m virgin and age-matched postpartum females, quantitative real-time PCR and Western blotting were car

162 In agreement with this finding, quantitative real-time PCR experiments demonstrated the

163 Consistent with these findings, quantitative real-time PCR (qRT-PCR) and enzyme-linked i

164 ntroduce a plasmonic photothermal method for quantitative real-time PCR, using gold bipyramids and li

165 se (GAPDH) and 18S ribosomal RNA (rRNA), for quantitative real-time PCR.

166 In this study, results from quantitative real-time PCR (qRT-PCR) assays are used as

167 Next-generation sequencing, 16 S rRNA gene quantitative real-time PCR, and aerobic culturing were a

168 gmentosum (XP) A and other DNA repair genes (quantitative real-time PCR analysis) and resulted in an

169 Here, quantitative real-time PCR analyses revealed that brains

170 ells and BAL fluid were evaluated for HPGDS (quantitative real-time PCR/immunohistochemistry [IHC]) a

171 t were examined using in situ hybridization, quantitative real-time PCR, Western blot analysis and fi

172 lonization by (i) classical culture and (ii) quantitative real-time PCR (qPCR) targetinglytAin patien

173 Immunohistochemistry, immunofluorescence, quantitative real-time PCR, in vivo pharmacology, Wester

174 We used immunostaining, quantitative real-time PCR, Western blots, and lung canc

175 onal antibodies coupled with mRNA isolation, quantitative real-time PCR, and standard PCR analyses co

176 ergillus-specific lateral-flow device (LFD), quantitative real-time PCR (qPCR), and the galactomannan

177 Padlock probe-mediated quantitative real time PCR (PLP-qRT-PCR) was adapted to

178 pithelium were determined by DNA microarray, quantitative real-time PCR, and immunohistochemistry bas

179 variety of approaches, including microarray, quantitative real-time PCR, Western blotting, chromatin

180 T cells was measured by means of microarray, quantitative real-time PCR, or both.

181 , such as bisulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectrosc

182 robial peptides was measured using multiplex quantitative real time PCR.

183 tudy for the first time explored a multiplex quantitative real-time PCR (qPCR) technique combined wit

184 escribe development of an absolute multiplex quantitative real-time PCR for detection of Plasmodium s

185 After adaptation to multiplex quantitative real-time PCR (qRT-PCR), the signature was

186 ion were assessed by comparative analysis of quantitative real-time PCR (qrtPCR) by the delta-delta C

187 The application of quantitative real-time PCR (qPCR) technologies for the r

188 We present an assay based on quantitative real-time PCR which estimates the relative

189 Based on microarray and/or quantitative real-time PCR and northern blot analyses, m

190 RT-PCR, quantitative real-time PCR and Western blotting were emp

191 Barr virus (EBV), were studied by performing quantitative real-time PCR analysis of blood samples obt

192 n of the genes that code identified protein, quantitative real time PCR (qRT-PCR) was performed.

193 K, which encodes an uncharacterized protein; quantitative real-time PCR (qRT-PCR) confirmed consisten

194 Relative quantitative real-time PCR (qRT-PCR) was used to determi

195 Here we show via relative quantitative real-time PCR and microarray analysis an in

196 d and 12 other FBLN5-regulated genes by semi-quantitative real time PCR.

197 ion in Golden Syrian hamsters by a sensitive quantitative real-time PCR (TaqMan) with lipl32 as the t

198 P was compared to that of a highly sensitive quantitative real-time PCR (qPCR) assay.

199 creening and treatment with highly sensitive quantitative real-time PCR, or MDA with blood-stage trea

200 herin at cell junctions; and (iii) sensitive quantitative real-time PCR assays detected HDV replicati

201 ence-activated cell sorting, RNA sequencing, quantitative real-time PCR, Western blotting, small inte

202 of HCL, we developed a BRAFV600Emut-specific quantitative real-time PCR assay and validated it in 117

203 More specifically, quantitative real time PCR (qPCR) achieves a high degree

204 associated with using the analogue standard, quantitative real-time PCR (qPCR).

205 resveratrol, TNF-alpha, and staurosporine), quantitative real-time PCR and clustering analysis, we s

206 Subsequent quantitative real-time PCR (qRT-PCR) and Northern blotti

207 r cholesterol-coated surfaces and subsequent quantitative real-time PCR (qRT-PCR) revealed that type

208 RNAs were selected and validated by targeted quantitative real-time PCR.

209 The quantitative real time PCR analysis was based on a suppr

210 The quantitative real-time PCR assay demonstrated that repai

211 These results were also confirmed by the quantitative real-time PCR (qPCR), droplet digital PCR (

212 panel of human tissues was analysed through quantitative real-time PCR methods, to quantify the rela

213 specific transcripts were identified through quantitative real-time PCR.

214 Using a high-throughput quantitative real-time PCR platform, we performed miRNA

215 re length was measured using high-throughput quantitative real-time PCR.

216 extraction method for reverse transcriptase quantitative real-time PCR (RT-qPCR)-based detection app

217 throughput, stem-loop, reverse transcriptase quantitative real-time PCR miRNA expression profiling (s

218 situ hybridization and reverse transcriptase quantitative real-time PCR.

219 ession was assessed by reverse transcription quantitative real time PCR and function by Hoechst 33342

220 s-specific primers for reverse transcription quantitative real-time PCR (RT-qPCR) were designed by id

221 6 mRNA, as shown using reverse-transcription quantitative real-time PCR and luciferase reporter activ

222 experiments, including in vitro translation, quantitative real-time PCR, Northern blot, ribonuclease

223 of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of trans

224 We also used quantitative real-time PCR to analyse a panel of cDNAs d

225 We used quantitative real-time PCR (qPCR) to test for 32 enterop

226 First, we used quantitative real-time PCR (qRT-PCR) to show that the sa

227 We used quantitative real-time PCR and IHC in human ICM and a ra

228 We used quantitative real-time PCR to analyze expression of neur

229 f the MCM family to GBM progression, we used quantitative real-time PCR to analyze the gene expressio

230 We used quantitative real-time PCR to determine DEFA1A3 DNA copy

231 vement in human cortical development we used quantitative real-time PCR to examine the expression tra

232 In this study, we used quantitative real-time-PCR to define miRNA expression pa

233 Using quantitative real time PCR and chromatin Immuno-Precipit

234 Using quantitative real time PCR, we have shown higher express

235 n and cellular responses were measured using quantitative real time PCR and multiplex assay.

236 , n = 59) and expression was validated using quantitative real time PCR (n = 21) and western blotting

237 Gene expression analysis using quantitative real time-PCR, GGPPS promoter-GUS (beta-glu

238 Using quantitative real-time PCR (q real-time PCR), YPEL5/PPP1

239 Using quantitative real-time PCR (qRT-PCR) we have measured th

240 Using quantitative real-time PCR (qRT-PCR), it showed that the

241 Using quantitative real-time PCR (QRT-PCR), we evaluated EBV i

242 Using quantitative real-time PCR analysis, we find that 13 for

243 Using quantitative real-time PCR and functional drug efflux st

244 Using quantitative real-time PCR of laser capture microdissect

245 Using quantitative real-time PCR, GSTP1 gene expression was as

246 Using quantitative real-time PCR, the expression of twenty-sev

247 Using quantitative real-time PCR, we found that transcript lev

248 Using quantitative real-time PCR, we investigated the intra-he

249 Using quantitative real-time PCR, we investigated the thermore

250 Using quantitative real-time PCR, we prospectively measured re

251 Using quantitative real-time PCR, we show that A. burtoni AR s

252 Using quantitative real-time PCR, we showed that the block to

253 ndent gene expression in P. aeruginosa using quantitative real-time PCR.

254 culture-independent molecular analysis using quantitative real-time PCR (qPCR).

255 in clonal beta-cell lines as assessed using quantitative real-time PCR and immunohistochemistry.

256 efined iron limitation was verified by using quantitative real-time PCR (qPCR).

257 characterized at higher resolution by using quantitative real-time PCR (qRT-PCR).

258 CCA was analyzed at 24 h or 21 d by using quantitative real-time PCR and immunohistochemistry.

259 measured in different human tissues by using quantitative real-time PCR and immunohistochemistry.

260 on of the TH17 response was studied by using quantitative real-time PCR, flow cytometry, and addition

261 By using quantitative real-time PCR, immunofluorescent detection

262 Results were validated by using quantitative real-time PCR, protein expression, and func

263 XMRV and monitored virus production by using quantitative real-time PCR.

264 status (E2/E6 ratio) was determined by using quantitative real-time PCR.

265 on DC subsets by HDM was quantified by using quantitative real-time PCR.

266 s, and mRNA expression was assessed by using quantitative real-time PCR.

267 This was confirmed using quantitative real-time PCR (n = 4).

268 growth-arrested embryos (degenerates) using quantitative real-time PCR (qRT-PCR).

269 y and gene transcripts were determined using quantitative real-time PCR.

270 nfirmation of gene expression was done using quantitative real-time PCR.

271 ures; these were validated empirically using quantitative real-time PCR to measure gene expression.

272 obacterium tuberculosis were evaluated using quantitative real-time PCR.

273 N) messenger RNA (mRNA), was evaluated using quantitative real-time PCR.

274 umber of the NADH dehydrogenase 1 gene using quantitative real-time PCR.

275 Enumeration of Dhc biomarker genes using quantitative real-time PCR (qPCR) in groundwater is a ke

276 s was done for a select group of genes using quantitative real-time PCR (qRT-PCR).

277 y and diseased tissue was investigated using quantitative real-time PCR.

278 mtDNA copy number was measured using quantitative real-time PCR on PBC DNA samples from parti

279 alpha-Synuclein mRNA levels, measured using quantitative real-time PCR, did not differ significantly

280 TNF]-alpha(KO), or caspase 7(KO) mice) using quantitative real-time PCR, enzyme-linked immunosorbent

281 yed for their increased levels of mRNA using quantitative real-time PCR.

282 t women and confirmed this observation using quantitative real-time PCR and mass spectrometry proteom

283 We validated the results obtained using quantitative real-time PCR (qPCR).

284 syndrome (MDS) and 76 healthy persons using quantitative real-time PCR.

285 pression in human tissues was profiled using quantitative real-time PCR.

286 ntrols and patients with schizophrenia using quantitative real-time PCR.

287 However, enumeration of spirochetes using quantitative real-time PCR revealed tissue-specific diff

288 Studies using quantitative real-time PCR revealed stage-specific chang

289 ved in predation process was validated using quantitative real-time PCR (qRT-PCR).

290 n a subset of the genes were validated using quantitative real-time PCR.

291 Functional validation using quantitative real-time PCR (qRT-PCR) indicated four prom

292 es (LN) and peripheral blood cells utilizing quantitative real-time PCR at different time points post

293 by miR-21 in human and mouse cells, whereas quantitative real-time PCR revealed little difference at

294 ssion events that are highly correlated with quantitative real time PCR measurement.

295 Compared with quantitative real-time PCR (qPCR), metagenomic sequencin

296 This finding was corroborated with quantitative real-time PCR assays and protein immunoblot

297 g chromatin immunoprecipitation coupled with quantitative real-time PCR, we identified five putative

298 eleted mtDNA in each donor was measured with quantitative real-time PCR (qPCR).

299 he KCNQ2 and KCNQ3 subunits as measured with quantitative real-time PCR.

300 onfirmed the results from array studies with quantitative real-time PCR analysis.