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1 rDNA silencing is one such mechanism that ensures the in
2 rDNA transcription and cell proliferation were sustained
8 entified using the ratio of 16S rRNA and 16S rDNA (rRNA/rDNA) for individual populations, but this ap
9 s, we performed nutritional analysis and 16S rDNA microbiome sequencing, which showed that high-fat d
10 rial markers (cytochrome oxidase COI and 16S rDNA) were employed for species identification of commer
11 on cohort, the correlation between blood 16S rDNA concentration and LF, whereas we did not confirm th
12 microbiome composition, assessed by both 16S rDNA and metagenomic DNA sequencing, of TB cases during
17 rase chain reaction and qualitatively by 16S rDNA targeted metagenomic sequencing and functional meta
22 ld of microbiome research is moving from 16S rDNA gene sequencing to a more comprehensive genomic and
24 rial DNA sequences of four genetic loci (16S rDNA, 28S rDNA, Cytochrome oxidase I and Cytochrome b).
26 ysis using next-generation sequencing of 16S rDNA showed significant shifts in the microbial communit
28 that predicts media given an organism's 16S rDNA sequence, facilitating future cultivation efforts.
29 We also demonstrate, based on in silico 16S rDNA analysis, that PMH isolates previously recovered fr
31 s of synthetic data corresponding to the 16S rDNA gene of bacteria, we show that our approach could b
32 cell sorting and deep sequencing of the 16S rDNA gene was used to characterize the bacterial recogni
37 lex melt curve signatures as compared to 16S rDNA amplicons with enhanced interspecies discrimination
40 characterization was performed by using 16S rDNA pyrosequencing of 872 nasal swabs collected biweekl
41 bolic models of microbes identified with 16S rDNA data to probe the ecology of microbial communities.
42 ifugation, genomic DNA was extracted and 16S-rDNA amplicons were subjected to high-throughput pyroseq
44 a data set of eukaryotic 454 sequencing 18S rDNA from the surveyed samples and showed significant di
45 ss this gap by analysing high-throughput 18S rDNA and 18S rRNA sequencing data from different Europea
47 lexed HyCCAPP was applied to four genes (25S rDNA, ARX1, CTT1, and RPL30) in S. cerevisiae under norm
48 nd ND5) and nuclear (5.8S rDNA, ITS2 and 28S rDNA) data from 419 individuals of Adelphocoris suturali
50 d (L-type) and separated (S-type) 5S and 35S rDNA units, chromosome number, genome size and ploidy le
57 ts of k-mers associated with centromere, 45S rDNA, knob, and retrotransposons were found among groups
58 upancy, including the 45S ribosomal DNA (45S rDNA) loci, where loss of ATRX results in altered expres
60 essed MAS2 and caused hypomethylation of 45S rDNA promoters as well as partial NOR decondensation, in
61 r than B73, equivalent levels of overall 45S rDNA expression indicates that transcriptional or post-t
70 three regions, and the lost ability of 5.8S rDNA sequence to fold into a conserved secondary structu
71 ndrial (COI, CYTB and ND5) and nuclear (5.8S rDNA, ITS2 and 28S rDNA) data from 419 individuals of Ad
75 rthermore, although the percentage of active rDNA repeats remains unaffected in the mutant cells, the
78 the distribution of RNase H1 and Top1 along rDNA coincided at sites where R-loops accumulated in mam
82 that occurs between the RPA135-tK(CUU)P and rDNA IGS1 loci stabilizes rDNA repeat number and contrib
83 mmunity in a subtropical estuary by rRNA and rDNA-based high throughput sequencing of 97 samples coll
85 the data show an inverse association between rDNA dosage and mitochondrial DNA abundance that is mani
86 x epistatic and allelic interactions between rDNA haplotypes that apparently regulate the entire rRNA
87 was exploited to study relationships between rDNA locus number, distribution, the occurrence of linke
89 including ribosomal DNA (rDNA) repeats, but rDNA methylation was strongly correlated with genetic va
92 tically, REGgamma-proteasome limits cellular rDNA transcription and energy consumption by targeting t
95 ines derived from T. mirus with a dominant d-rDNA homeolog transmitted this expression pattern over g
96 itors displaying reciprocal ND resulted in d-rDNA dominance, indicating immediate suppression of p-ho
99 imethylation and, correspondingly, decreases rDNA promoter DNA methylation, consistent with a role fo
103 imethylation at K232/254 by ESET deregulates rDNA transcription in a cell model of Huntington's disea
106 BCG (rBCG), electroporated recombinant DNA (rDNA) along with an interleukin-12 (IL-12)-expressing pl
108 , the unique structure of the ribosomal DNA (rDNA) array is thought to cause late SCI resolution of t
111 genes are found in two large ribosomal DNA (rDNA) clusters and little is known about the contributio
112 esidual replication stress on ribosomal DNA (rDNA) genes leads to the formation of nucleolar-associat
113 , and noncoding RNAs from the ribosomal DNA (rDNA) intergenic spacers, consistent with its previously
114 analyzed plastid and nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequence variati
116 nuclei, genetically unlinked ribosomal DNA (rDNA) loci are uniquely clustered together within the nu
120 ed both quantitatively by 16S ribosomal DNA (rDNA) quantitative polymerase chain reaction and qualita
121 the intergenic spacer of the ribosomal DNA (rDNA) repeats and the intergenic sequence between the lo
122 em repeat families, including ribosomal DNA (rDNA) repeats, but rDNA methylation was strongly correla
123 tely 12 million diatom V9-18S ribosomal DNA (rDNA) ribotypes, derived from 293 size-fractionated plan
124 Using small subunit (SSU) ribosomal DNA (rDNA) sequencing, we developed a targeted PCR protocol f
125 one deacetylase Sir2 controls ribosomal DNA (rDNA) silencing by inhibiting recombination and RNA poly
126 for SUMO-dependent control of ribosomal DNA (rDNA) silencing through the opposing actions of a STUbL
127 targeting of FGFR2, activate ribosomal DNA (rDNA) transcription and delay differentiation in osteopr
128 leolar protein that regulates ribosomal DNA (rDNA) transcription and is mutated in Treacher Collins s
130 that diseases with defects in ribosomal DNA (rDNA) transcription have mitochondrial dysfunction, and,
131 ion of EBV types 1 and 2; 16S ribosomal DNA (rDNA), a marker of microbial translocation; and HIV-1 RN
132 subunit (LSU) of the nuclear ribosomal DNA (rDNA), as well as fragments of the translation elongatio
133 During replication of nuclear ribosomal DNA (rDNA), clashes with the transcription apparatus can caus
138 Here, nonnative rRNA gene [ribosomal DNA (rDNA)] copies were identified in a set of 16 diploid bar
140 nterleukin-12 (IL-12)-expressing plasmid (EP rDNA plus pIL-12), yellow fever vaccine virus 17D (rYF17
144 tructure, and the high levels of euchromatic rDNA induced by the BBDS mutations direct nucleolar diso
147 C terminus that mediates its specificity for rDNA-associated proteins and show that this region binds
148 Ribosomal RNA (rRNA) is transcribed from rDNA by RNA polymerase I (Pol I) to produce the 45S prec
151 In this study, we compared 16S rRNA genes (rDNA) and rRNA gene expression of taxa in an activated-s
152 Approximately seven hundred 45S rRNA genes (rDNA) in the Arabidopsis thaliana genome are organised i
153 Uniparental silencing of 35S rRNA genes (rDNA), known as nucleolar dominance (ND), is common in i
157 ic modification of UBF is linked to impaired rDNA transcription and nucleolar chromatin remodeling, w
159 2 loss of function triggers major changes in rDNA spatial organization, expression, and transgenerati
162 eviously uncharacterized function of NuMA in rDNA transcription and p53-independent nucleolar stress
166 trongly correlated with genetic variation in rDNA copy number and was not influenced by paternal diet
167 The analyses uncover abundant variation in rDNA dosage that is coupled with the expression of hundr
171 e promoted chromosome kissing that initiated rDNA recombination and controlled the replicative life s
174 host tissues predominantly belonged to ITS2 rDNA type C3 in the Persian Gulf and type D1a in the Sea
178 (mURA3) integrated adjacent to the leftmost rDNA gene to investigate localized Pol I and Fob1 functi
180 t condensin plays a dual role in maintaining rDNA stability: it suppresses the formation of Spo11-med
181 served Pir2/ARS2 protein, and also maintains rDNA integrity and silencing by promoting heterochromati
183 t suppresses the formation of Spo11-mediated rDNA breaks, and it promotes DSB processing to ensure pr
185 cess and subsequently replaced by a modified rDNA unit used to regenerate rDNA at three distinct chro
186 in in the nucleus and predicts that multiple rDNA loci will form a single nucleolus independent of th
187 re was no evidence that any of the nonnative rDNA units were transcribed; some showed indications of
188 vide mechanistic insights into how the novel rDNA transcription repressor BEND3 acts together with No
194 erences in predictions of multiple copies of rDNA operons for each respective bacterium were evaluate
196 rk barrier protein is situated downstream of rDNA, there preventing replication in the direction oppo
198 udied the genetic and epigenetic features of rDNA homeologs in several lines derived from recently an
201 which are known to have a reduced number of rDNA copies, and plant lines with restored CAF-1 functio
202 t chromosome length, and not the presence of rDNA repeats, is the critical feature determining the ti
203 mechanistic insights into the regulation of rDNA silencing and intrachromatid recombination by showi
207 ying FGFR2 as a transcriptional regulator of rDNA in bone unexpectedly reveals a nucleolar route for
210 ion marking the transcriptional silencing of rDNA genes and decreased ribosome biogenesis in quiescen
211 about a change in the chromatin structure of rDNA and thus plays an important role in linking TOR sig
212 present genetic evidence that this effect on rDNA recombination, but not silencing, is entirely depen
214 n factor upstream binding factor 1 (UBF1) on rDNA promoters and recruiting Rac1 and its downstream ef
216 unaffected in the mutant cells, the overall rDNA copy number increases ~2-fold compared with WT.
220 viduals harbor up to five different panicoid rDNA units in addition to the native Hordeum rDNA copies
221 ts that inherited the corresponding parental rDNA genotype, thus indicating the generation of additio
222 ing we assign these variants to a particular rDNA cluster unambiguously and use them as reporters of
223 able epigenetic reprogramming of the partner rDNA arrays, harmonizing the expression of thousands of
224 of2 abundance, suggesting that Ulp2 promotes rDNA silencing by opposing STUbL-mediated degradation of
225 ith either Ulp2 or Tof2 dramatically reduces rDNA silencing and causes a marked drop in Tof2 abundanc
226 on and degradation, thereby further reducing rDNA transcription to save energy to overcome cell death
228 lized SUMO isopeptidase and a STUbL regulate rDNA silencing by controlling the abundance of a key rDN
229 rring endonuclease that targets a repetitive rDNA sequence highly-conserved in a wide range of organi
230 ith chromosome segregation at the repetitive rDNA sequence, thereby maintaining genome integrity.
231 ival during energy starvation via repressing rDNA transcription, a major intracellular energy-consumi
233 ing the ratio of 16S rRNA and 16S rDNA (rRNA/rDNA) for individual populations, but this approach fail
240 analyses of peritrichs that incorporated SSU rDNA sequences of samples collected from three continent
241 insea clade, encompassing a low level of SSU rDNA variation different from the lineage previously ass
242 PA135-tK(CUU)P and rDNA IGS1 loci stabilizes rDNA repeat number and contributes to the maintenance of
244 haemulonii, and Candida lusitaniae Targeting rDNA region nucleotide sequences, primers specific for C
245 age varies greatly among accessions and that rDNA cluster-specific expression and silencing is contro
247 mation of lethal repair intermediates at the rDNA because of a barrier imposed by RNA polymerase I.
248 grity, demonstrating that the defects at the rDNA can be directly attributed to loss of cohesion.
250 mechanisms promoting genome stability at the rDNA locus and subtelomeric regions in the most common h
261 polymerase II-catalyzed transcription in the rDNA of Saccharomyces cerevisiae Sir2 is recruited to no
262 ences and frequent rearrangements inside the rDNA loci have generated considerable interspecific and
263 ted to nontranscribed spacer 1 (NTS1) of the rDNA array by interaction between the RENT ( RE: gulatio
265 Pol I occupancy of the coding region of the rDNA in THO mutants is decreased to ~50% of WT level.
266 ts strongly suggest that organization of the rDNA provided by cohesion is critical for formation and
267 ed a dramatic reduction in the number of the rDNA repeat units in Spirodela to fewer than 100, which
269 e CIP-box motifs of the Dna2 nuclease or the rDNA-associated protein Tof2 do not perturb DNA synthesi
270 cts with Ulp2 and one of its substrates, the rDNA silencing protein Tof2, through adjacent conserved
271 tion and energy consumption by targeting the rDNA transcription activator SirT7 for ubiquitin-indepen
272 a model whereby Top1p recruits Sir2p to the rDNA and clarifies a structural role of DNA topoisomeras
274 ing by markedly increased UBF binding to the rDNA promoter and to the 5'- external transcribed spacer
277 omethylated chromatin is associated with the rDNA locus while telomeric regions are assembled into a
278 ound that THO physically associates with the rDNA repeat and interacts genetically with Pol I transcr
280 utant vegetative cell nuclei, however, these rDNA loci frequently colocalized with condensed centrome
284 remains into adulthood and is restricted to rDNA copies associated with a specific genetic variant w
285 ave disproportionally high ratios of rRNA to rDNA, an indication of higher protein synthesis, compare
286 n important role in linking TOR signaling to rDNA transcription and ribosome biogenesis in plants.
292 he nucleolus, DDX21 occupies the transcribed rDNA locus, directly contacts both rRNA and snoRNAs, and
294 o study the relationship between uniparental rDNA (encoding 18S, 5.8S and 26S ribosomal RNA) silencin
295 , we show for the first time that unresolved rDNA sequence variation resulting from imperfect concert
300 -transcribed genes embedded within the yeast rDNA locus are repressed through a Sir2-dependent proces
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