ライフサイエンスコーパス: rRNA

1 ing, and reduced mitochondrial function, 12S rRNA expression, and SMAD7 expression, consistent with t

2 n patients with ICUAW, the expression of 12S rRNA and of the inhibitors of SMAD2/3 phosphorylation we

3 16S rRNA amplicon pyrosequencing showed an increase in the r

4 16S rRNA amplicon sequencing showed that the genus Arthrobac

5 16S rRNA amplicon sequencing was combined with GeoChip metag

6 16S rRNA analyses of field-derived biofilm indicated the dom

7 16S rRNA gene quantification and sequencing revealed that gr

8 16S rRNA sequencing revealed significant increases in specie

9 yi cell densities were 10(13) and 10(12) 16S rRNA gene copies L(-1) in the bioflocs and planktonic cu

10 H 5.5 enrichment shared 98.6%, and 98.5% 16S rRNA gene sequence similarities to Sulfurospirillum mult

11 d MGII in PRE (up to approximately 10(8) 16S rRNA gene copies/l), which was around 10-fold higher tha

12 lated to Thermodesulfovibrio sp. (87-89% 16S rRNA gene identity, 52-54% average amino acid identity),

13 ed of three named species that share 99% 16S rRNA gene sequence identity.

14 Combining a 16S rRNA (16S) gene database with metagenomic shotgun sequen

15 after seawater acclimatisation (SW) by a 16S rRNA (V3-V4) high-throughput sequencing approach.

16 transfers on CM and H2 , Acetobacterium 16S rRNA gene sequences dominated the culture and the DCM-de

17 t fermenters, we performed culturing and 16S rRNA gene amplicon sequencing on samples collected from

18 Metagenomics and 16S rRNA gene amplicon sequencing revealed the dominant flan

19 e gene of class 1 integrons (intI1), and 16S rRNA genes were quantified using quantitative polymerase

20 eks and analyzed by quantitative PCR and 16S rRNA sequencing.

21 V4 region of both bacterial and archaeal 16S rRNA gene was used to characterize the microbial communi

22 ing to similarities in key genes such as 16S rRNA and polyketide synthase genes.

23 ely been due to new technologies such as 16S rRNA sequencing to identify previously unknown microbial

24 fy mucosa-associated microbes, bacterial 16S rRNA and fungal ITS sequencing were performed on chronic

25 in metabolically active cells, bacterial 16S rRNA sequence plays an important role in microbial ident

26 licon sequencing targeting the bacterial 16S rRNA V3-V4 region and the cyanobacterial ntcA gene.

27 oportions of microbiota were analyzed by 16S rRNA amplicon sequencing and quantitative PCR.

28 autotrophy, based on characterization by 16S rRNA gene amplicon sequencing and respiratory quinone co

29 setting, fecal microbiota, evaluated by 16S rRNA gene amplicon sequencing, shifted to a state of red

30 sition of the microbiota was analyzed by 16S rRNA sequencing and quantitative polymerase chain reacti

31 By 16S rRNA sequencing, we show here that diabetes causes a shi

32 assemble on the 5' domain of the E. coli 16S rRNA.

33 Ds and sequencing of the almost complete 16S rRNA gene.

34 duced and were hybridized with a defined 16S rRNA region on the long DNA-RNA sandwich assemblies, res

35 formation, analysis of the MiSeq-derived 16S rRNA data revealed dramatic and differential shifts in t

36 mentation of this strategy for detecting 16S rRNA sequences in total RNA mixed samples extracted from

37 that progressively stabilize the folded 16S rRNA.

38 sting taxonomic classification tools for 16S rRNA gene sequences either do not provide species-level

39 Species-level classification for 16S rRNA gene sequences remains a serious challenge for micr

40 treatment and 5 days after treatment for 16S rRNA gene sequencing.

41 acterial metagenomes were predicted from 16S rRNA data by using Phylogenetic Investigation of Communi

42 Results from 16S rRNA gene amplicon analysis and metagenomics suggested t

43 its utilization of short reads only from 16S rRNA genes, not from entire genomes.

44 thology and molecular diagnostics (e.g., 16S rRNA gene PCR/sequencing, Tropheryma whipplei PCR) may b

45 In 16S rRNA gene Illumina libraries, four Pseudomonas sp. opera

46 increased spleen levels of Lactobacillus 16S rRNA in SDR mice positively correlated with increased le

47 lines Initiative (6,177 near full-length 16S rRNA gene sequences and 9.4 million high-quality 16S V1-

48 Notably, methanogen 16S rRNA signatures were absent in all Illumina libraries an

49 Microbial 16S rRNA metagenomic sequencing analyses were then used to i

50 y one of the largest published microbial 16S rRNA sequence dataset.

51 osition U1369 in the human mitochondrial 16S rRNA.

52 at McElmo Dome, Colorado for analysis of 16S rRNA gene diversity and metagenome content.

53 Unsupervised clustering of 16S rRNA gene sequences revealed three clusters (subtypes),

54 biota were analyzed by pyrosequencing of 16S rRNA genes and enumeration of selected bacteria by cultu

55 The relative abundance of 16S rRNA genes was measured by Quantitative Polymerase Chain

56 Processing of 16S rRNA is also delayed in the mutant strain, as indicated

57 With the advent of 16s rRNA sequencing replacing traditional culture methods, a

58 ibute to the folding of the 3' domain of 16S rRNA.

59 deep-branching plastid lineages based on 16S rRNA gene data.

60 ined from deep-sea environments based on 16S rRNA gene similarity and BLAST matches to predicted prot

61 Our 16S rRNA gene pyrosequencing analyses revealed that the soil

62 r, the limitations of relying on partial 16S rRNA for discrimination of Edwardsiella spp. and advanta

63 ngside the bacteria with high-resolution 16S rRNA gene sequencing, linking these community data to ge

64 stnatal windows, we used high-resolution 16S rRNA marker gene sequencing to examine outcomes in our m

65 80 cores, DACE clustered the Lake Taihu 16S rRNA gene sequencing data ( approximately 316M reads, 30

66 CR amplification was performed targeting 16S rRNA/tRNA(val) region having an amplicon size of 530bp u

67 The targets 16S rRNA were selectively captured at the interface of the b

68 plementary sequence in the 3' end of the 16S rRNA (anti-Shine-Dalgarno sequence [aSD]).

69 rption/ionization-time-of-flight and the 16S rRNA gene for identification, we cultured 329 new bacter

70 as analyzed by 454 pyrosequencing of the 16S rRNA gene fragments.

71 cies based on melt-curve profiles of the 16S rRNA gene in an automated fashion.

72 applicable for different regions of the 16S rRNA gene or other phylogenetic marker genes.

73 diversity and absolute abundance of the 16S rRNA gene per location.

74 q sequencing of the V1-V3 regions of the 16S rRNA gene used to compare VM composition.

75 lated DNA was assessed by amplifying the 16S rRNA gene using Com1 and Com2 universal primers.

76 ng next-generation DNA sequencing of the 16S rRNA gene, we analyzed the composition and diversity of

77 irmicutes metagenomes predicted from the 16S rRNA gene.

78 biome was investigated by sequencing the 16S rRNA gene.

79 es due to the strong conservation of the 16S rRNA gene.

80 icrobiota was assessed by sequencing the 16S rRNA gene.

81 The 16S rRNA sequences for each isolate were matched to culture-

82 encing analysis of the gene encoding the 16S rRNA were performed to interrogate the composition and f

83 PCR targeting the 16S rRNA-encoding gene and chaperonin-60 (cpn60) showed that

84 t aggressive in MPn metabolism and their 16S rRNA gene sequences matched 35% of the Illumina PMEZ Pse

85 s, while diverging by only 1.1% in their 16S rRNA genes, have evolved systematic differences in metab

86 he rat digestive tract were subjected to 16S rRNA gene sequencing-based analysis to determine the bas

87 alysis of the nucleotide sequence of two 16S rRNA regions showed that wAnga-Mali clusters with Wolbac

88 Our prior work uncovered 16S rRNA genes belonging to a novel, as-yet-uncultivated myc

89 Microbiome studies commonly use 16S rRNA gene amplicon sequencing to characterize microbial

90 We used 16S rRNA amplicon sequencing to characterize the global patt

91 We used 16S rRNA gene polymerase chain reaction with degenerate prim

92 We used 16S rRNA sequencing to quantify the relative effects of samp

93 and laboratory tsetse populations using 16S rRNA gene amplicon sequencing allowed us to examine whet

94 legume housed Micromonospora, and using 16S rRNA gene sequence identification, we verified that the

95 s study, we profile gut microbiota using 16S rRNA gene sequencing in 531 well-phenotyped Finnish men

96 Oral bacteria were assessed using 16S rRNA gene sequencing in prediagnostic mouthwash samples

97 ial communities were characterized using 16S rRNA gene sequencing.

98 control subjects were profiled by using 16S rRNA gene sequencing.

99 tively and weekly during gestation using 16S rRNA gene sequencing.

100 controlled trial was determined by using 16S rRNA gene sequencing.

101 of time and electrical performance using 16S rRNA gene-based phylogenetic microarrays and flow cytome

102 l pellets from various populations using 16S rRNA sequencing to investigate structuring of microbial

103 y, larynx-pharynx, and lymph nodes using 16S rRNA sequencing.

104 V4 16S rRNA gene sequencing of fecal DNA demonstrated minimal s

105 on (Illumina) sequencing of partial (V4) 16S rRNA gene sequences.

106 s (92.6%) and Proteobacteria (6.9%), via 16S rRNA gene amplicon sequencing.

107 subgingival microbiome was evaluated via 16S rRNA gene sequencing and 8 selected inflammatory markers

108 subject; in total, n = 1,121 samples) by 16S-rRNA gene amplicon sequencing.

109 Sequencing of the 16S-rRNA components of this aerosol revealed a microbiome de

110 dicated by increased levels of precursor 17S rRNA in assembly intermediates.

111 S rRNA percent, 18S rRNA percent, and 5S-18S rRNA ratio, using close relationship between volume perc

112 er confirmed that 5S rRNA percent and 5S/18S rRNA ratio can serve as markers to distinguish sexes una

113 In metagenomics, 16S and 18S rRNA gene have been widely used as marker genes to profi

114 in 25 min, and the Ocean TARA Eukaryotic 18S rRNA gene sequencing data ( approximately 500M reads, 88

115 motifs in human hUTP23 are essential for 18S rRNA maturation.

116 d regions of four genetic markers (ITS1, 18S rRNA, 28S rRNA and COI) for their ability to differentia

117 rategy, allowing a reduction of mosquito 18s rRNA gene sequences by more than 80% for the V4 hypervar

118 platform for the separate maturation of 18S rRNA domains.

119 (rRNA) indexes, namely 5S rRNA percent, 18S rRNA percent, and 5S-18S rRNA ratio, using close relatio

120 ctors regulate the timely folding of pre-18S rRNA.

121 used high throughput methods to sequence 18S rRNA, cloned and sequenced 28S rRNA and microscopically

122 o1 coordinates the 3'end cleavage of the 18S rRNA by Nob1 and how the late factor's removal in the cy

123 s with expansion sequence 6 (ES6) in the 18S rRNA sequence and that yUtp23 interacts with the 3 half

124 Based on analysis of the 18S rRNA sequences, we showed that fungal taxa represented b

125 ed to map modifications found in 16S and 23S rRNA from Streptomyces griseus.

126 tions of ponA, porB, gyrA, and parC; and 23S rRNA sequences.

127 reover, M. tuberculosis MazF-mt6 cleaves 23S rRNA Helix 70 to inhibit protein synthesis.

128 rmal assay targets the same Enterococcus 23S rRNA gene region as the existing quantitative polymerase

129 100-nt long expansion segment of the Mtb 23S rRNA, named H54a or the 'handle', switches interactions

130 arm of deacylated tRNA with helix 68 of 23S rRNA.

131 ng mutations in nucleotides 2600-2605 of 23S rRNA; these had enhanced sensitivity to the phosphorylat

132 n parallel was the re-engineering of the 23S rRNA of Escherichia coli, guided by the use of a phospho

133 nt of nucleotides U2506 and U2585 of the 23S rRNA resulting in the formation of the U2506*G2583 wobbl

134 ferent assembly factors with domain V of 25S rRNA, including the neighborhood surrounding the peptidy

135 tment with the outcome that pre-rRNA and 28S rRNA levels also increased.

136 ue in the conserved sarcin/ricin loop of 28S rRNA.

137 of four genetic markers (ITS1, 18S rRNA, 28S rRNA and COI) for their ability to differentiate between

138 sequence 18S rRNA, cloned and sequenced 28S rRNA and microscopically counted chytrid-infected diatom

139 and D2 and D3 expansion segments of the 28S rRNA gene did not clarify the phylogeny at the genus lev

140 NuMA also binds to 18S and 28S rRNAs and localizes to rDNA promoter regions.

141 In Arabidopsis thaliana, 45S rRNA genes are found in two large ribosomal DNA (rDNA) c

142 three ribosome RNA (rRNA) indexes, namely 5S rRNA percent, 18S rRNA percent, and 5S-18S rRNA ratio, u

143 We further confirmed that 5S rRNA percent and 5S/18S rRNA ratio can serve as markers

144 uired for the maturation of the 23S and 4.5S rRNAs.

145 ately 7.3 million mRNA, 255 524 tRNA, 40 649 rRNA (different subunits) and 5250 miRNA, 3747 snRNA, ge

146 nce of aberrant nuclear accumulation of 5.8S rRNA in La cKO is supported by a 10-fold increase in a p

147 upported by a 10-fold increase in a pre-5.8S rRNA intermediate.

148 ntersubunit coordination, including the 5.8S rRNA.

149 O-methylation (2'-O-Me) is the most abundant rRNA chemical modification, and displays a complex patte

150 ncies, and report that Tau depletion affects rRNA synthesis, ribonucleotide pool balance, and rDNA st

151 ng those involved in ribosome biogenesis and rRNA processing.

152 wth stage oocytes or gonadosomatic index and rRNA content, demonstrating species-specific quantificat

153 We benchmark Structure-seq2 on both mRNA and rRNA structure in rice (Oryza sativa).

154 misia puparium, including complete mtCOI and rRNA genes, and various partial mtDNA genes.

155 teins (rProteins), and that the rProtein and rRNA are co-chaperones.

156 Protein, folding is independent of rRNA, and rRNA association is predominantly by nonelectrostatic me

157 ession profiles from both polyA-selected and rRNA-depleted libraries.

158 dhood with altered rDNA chromatin status and rRNA metabolism.

159 RNAs besides tRNA and rRNA contain chemical modifications, including the recen

160 aled that it cross-links to tRNAs, mRNAs and rRNAs, thereby placing the protein on translating riboso

161 t6 selectively cleaves both plant and animal rRNA species, and is toxic to wheat, tobacco, bacterial

162 The model predicts that appropriate rRNA fragments have inherited local autonomy of folding

163 y anticodons, or non-tRNA substrates such as rRNA, exhibiting the most dramatic translational perturb

164 studies, comparative evaluation of bacterial rRNA depletion methods has remained limited.

165 dimer is formed through interactions between rRNA h26, h40, and protein uS2, involving conformational

166 ampens fluctuations at the interface between rRNA subdomains where bS16 binds.

167 iliate community in a subtropical estuary by rRNA and rDNA-based high throughput sequencing of 97 sam

168 Here we challenged rRNA 2'-O-Me globally by inhibiting the rRNA methyl-tran

169 the 1001 Genomes Consortium, we characterize rRNA gene sequence variation within and among accessions

170 atasets uncover hidden breaks in chloroplast rRNA and identify a previously unreported N1-methyladeno

171 its tail with Domain III and with DIII(core) rRNA.

172 in its absence, levels of the corresponding rRNA processing intermediate are strongly increased, imp

173 established from DBA patients show defective rRNA processing and ribosomal stress features such as re

174 h simulated and real ribosomal RNA-depleted (rRNA-depleted) RNA-seq datasets.

175 driving mature rRNA formation and discarded rRNA decay.

176 tein-rRNA as well as long-range inter-domain rRNA interactions.

177 tress and DNA damage caused by mTORC1-driven rRNA synthesis, which renders nucleotide pools limiting.

178 concerted down-regulation of genes encoding rRNA and ribosomal proteins.

179 termine the flexibility of nearly the entire rRNA component of the yeast ribosome through 8 discrete

180 plotypes that apparently regulate the entire rRNA gene cluster.

181 Our data establish rRNA 2'-O-Me plasticity as a mechanism providing functio

182 regulators of pre-rRNA processing needed for rRNA maturation were also up-regulated after EX-527 trea

183 ranscripts in expression quantification from rRNA-depleted RNA-seq data showed substantial increased

184 tentially higher rRNA:mRNA ratios and higher rRNA carryover during RNA-seq analysis.

185 rial biofilms, which have potentially higher rRNA:mRNA ratios and higher rRNA carryover during RNA-se

186 cells, as measured by the reduction of human rRNA depurination detected by our novel TaqMan-based RT-

187 th the near-basal position of the Archaea in rRNA phylogenies.

188 e a nucleolar isoform has been implicated in rRNA biogenesis by multiple independent lines of evidenc

189 mN, which methylates specific nucleotides in rRNA and tRNA.

190 ification, and displays a complex pattern in rRNA.

191 units, while on a subset of genes, including rRNA and CRISPR loci, Spt4/5 is recruited to the transcr

192 d in many functional RNA molecules including rRNA, tRNA, snRNA and ribozymes.

193 provide nucleotide resolution insights into rRNA structural rearrangements during nucleolar 60S asse

194 multiple pre-rRNA cleavages, driving mature rRNA formation and discarded rRNA decay.

195 dy-state level of the 16S mitochondrial (mt) rRNA with defects in the biogenesis of the mitoribosome

196 While the aberrant adenylation of 16S mt-rRNA did not affect the integrity of the mitoribosome, s

197 components of electron transport and 16S mt-rRNA, similar to the phenotype observed in cells deficie

198 on of the 3' ends of the mitochondrial (mt-) rRNA and mt-tRNA.

199 The native rRNA conformation is increasingly favored after bS16 bin

200 Overall, there is less than one copy of non-rRNA per EV.

201 Here, nonnative rRNA gene [ribosomal DNA (rDNA)] copies were identified

202 Collectively, our data revealed a novel rRNA methylation mechanism by a radical SAM superfamily

203 Comprehensive datasets of rRNA base flexibilities provide a unique resource to the

204 hould realize that insufficient depletion of rRNA will probably lead to incorrect quantification of g

205 was demonstrated by the direct detection of rRNA in bacterial lysate.

206 n of the rProtein, folding is independent of rRNA, and rRNA association is predominantly by nonelectr

207 ion, which could be rescued by inhibition of rRNA processing or ER stress.

208 y four different assembly quality, number of rRNA and tRNA genes, and the occurrence of conserved fun

209 phism we discovered implies that the pool of rRNA in a cell may be heterogeneous, which could have fu

210 Suppression of rRNA synthesis during IGF-1 treatment did not prevent ea

211 Eukaryotic ribosomes are composed of rRNAs and ribosomal proteins.

212 e-mRNA splicing as well as the maturation of rRNAs.

213 xins that target nucleic acids (DNA, tRNA or rRNA) in the cytoplasm of susceptible bacteria, usually

214 results, we conclude that RH50 is a plastid rRNA maturation factor.

215 he ITS1 spacer can be removed from 27SA3 pre-rRNA.

216 The nuclear exosome is a key factor for pre-rRNA processing through the activity of its catalytic su

217 (RNP) from HeLa cells cleaves the human pre-rRNA in vitro at at least one site used in cells, while

218 Thus, a role for RNase MRP in human pre-rRNA processing is established.

219 Deleting gamma2-AMPK led to increases in pre-rRNA level, ER stress markers, and cell death during glu

220 and RNA sequencing-implicates MRP RNA in pre-rRNA processing.

221 Specifically, proteins involved in pre-rRNA transcription, including subunits of the polymerase

222 ease XRN2, a key coordinator of multiple pre-rRNA cleavages, driving mature rRNA formation and discar

223 p1 response pathway and leads to nascent pre-rRNA reduction.

224 Amelioration of pre-rRNA imbalance is achieved through rescue of MRP RNA lev

225 The subsequent downregulation of pre-rRNA level led to attenuated endoplasmic reticulum (ER)

226 many protein effectors and regulators of pre-rRNA processing needed for rRNA maturation were also up-

227 nesis, functioning in pre-ribosomal RNA (pre-rRNA) processing as a component of the small ribosomal s

228 cedure that minimizes pre-ribosomal RNA (pre-rRNA) transcripts.

229 ranslocates into the nucleus to suppress pre-rRNA transcription and ribosome biosynthesis during stre

230 r EX-527 treatment with the outcome that pre-rRNA and 28S rRNA levels also increased.

231 ably, variation is not restricted to the pre-rRNA sequences removed during processing, but it is also

232 plays a pivotal role in remodeling this pre-rRNA region in both yeast and humans.

233 esults in the appearance of intermediate pre-rRNAs species that reflect the processing of pre-rRNAs t

234 s species that reflect the processing of pre-rRNAs through Pathway 2, a pathway that processes pre-rR

235 ough Pathway 2, a pathway that processes pre-rRNAs in a different temporal order than the more often

236 insights into the temporal order of protein-rRNA as well as long-range inter-domain rRNA interaction

237 edictions about the establishment of protein-rRNA interactions, providing intriguing insights into th

238 pC7, VapC8) substantially degraded M. prunae rRNA in vitro.

239 hese VapCs mapped to motifs within M. prunae rRNA.

240 Thus, Ect2 regulates rRNA synthesis through a PKCiota-Ect2-Rac1-NPM signaling

241 ribonuclease nucleolar levels and regulating rRNA processing.

242 To remove rRNA and enrich coding sequences, subtractive hybridizat

243 tail region of the protein, folding requires rRNA, and association is predominantly by electrostatic

244 ionally relevant in ribozymes, riboswitches, rRNA, and during replication.

245 Ribosomal RNA (rRNA) accounts for the majority of the RNA in eukaryotic

246 donucleolytic cleavage in 25S ribosomal RNA (rRNA) adjacent to the c loop of the expansion segment 7

247 uilt de novo and includes 15S ribosomal RNA (rRNA) and 34 proteins, including 14 without homologs in

248 Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtei

249 rotein MRPS10 and reduced 12S ribosomal RNA (rRNA) expression, suggesting mitochondrial ribosomal str

250 iversity (small subunit (SSU) ribosomal RNA (rRNA) gene sequences) in field samples.

251 icoverpa zea larvae using 16S ribosomal RNA (rRNA) gene sequencing and matrix-assisted laser desorpti

252 The resulting accumulation of ribosomal RNA (rRNA) precursor-analyzed by RNA fluorescent in situ hybr

253 ined the relationship between ribosomal RNA (rRNA) production and IGF-1-mediated myotube hypertrophy

254 detail, little is known about ribosomal RNA (rRNA) structural rearrangements that take place during e

255 tified a role for this GEF in ribosomal RNA (rRNA) synthesis that is mediated by Rac1 and PKCiota-dep

256 at1-Rai1 to process precursor ribosomal RNA (rRNA), yet its mechanism of action remains unknown.

257 d modification of nascent pre-ribosomal RNA (rRNA).

258 assembly of the large-subunit ribosomal RNA (rRNA).

259 en ovary development and three ribosome RNA (rRNA) indexes, namely 5S rRNA percent, 18S rRNA percent,

260 Ribosomal RNAs (rRNAs) are main effectors of messenger RNA (mRNA) decodi

261 in the UASB reactors and identified by 16 S rRNA analysis.

262 re was characterized by high-throughput 16 s rRNA gene amplicon sequencing.

263 n of the gut microbiota was analyzed by 16 S rRNA gene high-throughput sequencing, and anxiety-like b

264 Using 16 S rRNA gene sequencing we demonstrated that microbiota eco

265 Culture and 16 S rRNA gene sequencing were performed on nasopharyngeal sp

266 Prokaryotic 16 S rRNA gene was amplified and DGGE was performed.

267 This study used DNA barcoding and 16 S rRNA sequencing as a method to identify shark and ray sp

268 p on d 0, 14, and 28 of feeding through 16 S rRNA sequencing.

269 g., fluorescence in situ hybridization, 16-S rRNA gene amplicon sequencing), yet high-throughput meth

270 sine (m1A) in a nuclear-encoded Oryza sativa rRNA.

271 Here, we sequenced rRNA genes from European and Japanese fish that are know

272 Yeast-specific rRNA and protein elements, including the acquisition of

273 Each sample was sequenced (16S SSU rRNA genes, average 10,000 reads), and biogeochemical pa

274 lyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT

275 and showed that despite their identical SSU rRNA sequences the strains had markedly different proper

276 In addition, detection of SSU rRNA and mcrA transcripts from one hot spring suggested

277 It is thus clear that SSU rRNA based operational taxonomy units, even at the most

278 ina pygmaea and Roccella fuciformis with SSU rRNA gene sequences identical to the type strain of Stre

279 diversity studies using small subunit (SSU) rRNA gene sequences continue to advance our understandin

280 tif and extra base-pairing, which stimulates rRNA 2-O-methylation, are both critical for multiple mod

281 mplification process combined with targeting rRNA to exploit phylogenetic differences for sensitive a

282 We show that rRNA gene cluster expression is controlled via complex e

283 s with cultured fibroblasts, we propose that rRNAs not packaged into complete ribosomal subunits are

284 demonstrate that, in the absence of hpf, the rRNA abundances of starved cells decrease to levels that

285 data argue that many distant domains in the rRNA can assemble simultaneously during early 60S assemb

286 that specific protein-induced changes in the rRNA dynamics underlie the hierarchy of 30S assembly and

287 nged rRNA 2'-O-Me globally by inhibiting the rRNA methyl-transferase fibrillarin in human cells.

288 o test these predictions, we investigate the rRNA interactions of rProtein uL23 and its tail, uL23(ta

289 uL23(tail) associates with Domain III of the rRNA and a subdomain called "DIII(core)".

290 olecule FRET to show how the dynamics of the rRNA dictate the order in which multiple proteins assemb

291 mal internal transcribed spacer (ITS) of the rRNA gene with fungal specific ITS primers, ITS barcodes

292 inds both native and non-native forms of the rRNA.

293 le ratio-based correction was applied to the rRNA-depleted estimates.

294 orted into the nucleus for assembly with the rRNAs.

295 Abundant noncoding RNAs such as tRNAs, rRNAs, and spliceosomal RNAs are also heavily modified a

296 However, whether rRNA 2'-O-Me is an adjustable feature of the human ribos

297 he ion dependences of their association with rRNA.

298 mmuno-positive staining that correlated with rRNA transcription, as shown by propidium iodide stainin

299 he 'handle', switches interactions from with rRNA helix H68 and rProtein uL2 to with rProtein bS6, fo

300 table association of ribosomal proteins with rRNA surrounding the polypeptide exit tunnel.