ライフサイエンスコーパス: random mutagenesis

1 y higher probability than those generated by random mutagenesis.

2 unction, we generated new avrRpt2 alleles by random mutagenesis.

3 mutant M3 receptor that included TM V-VII to random mutagenesis.

4 ssential genes with a subsaturation level of random mutagenesis.

5 itutively active mutant (CAM) was derived by random mutagenesis.

6 tic efficiency of variant GghA(s) induced by random mutagenesis.

7 st identified in combinations, as allowed by random mutagenesis.

8 ugh a combination of protein engineering and random mutagenesis.

9 d mutants, E165D and H95N, were subjected to random mutagenesis.

10 anges were made in the amino-TRD of EcoKI by random mutagenesis.

11 brain serotonin transporter was subjected to random mutagenesis.

12 40) using a combination of site-directed and random mutagenesis.

13 been subjected to both site-directed and PCR random mutagenesis.

14 ructure and function using site-directed and random mutagenesis.

15 the costs of further evolution by sequential random mutagenesis.

16 ransfer and polymerase chain reaction-driven random mutagenesis.

17 ifferent strategies - histidine scanning and random mutagenesis.

18 in activation function or among mutants from random mutagenesis.

19 n in the highly leukotoxic strain JP2N using random mutagenesis.

20 N6, and N8 NA subtypes using gene-fragmented random mutagenesis.

21 Following random mutagenesis, a temperature-sensitive strain exhib

22 gn, single-residue saturation mutagenesis or random mutagenesis, along with multiplex assembly, we id

23 five loss-of-function mutations arising from random mutagenesis alter residues that are highly conser

24 he functional domains of TLR2 we performed a random mutagenesis analysis of the human TLR2 TIR domain

25 ted secretion-deregulated IpaB mutants using random mutagenesis and a genetic screen.

26 ate a functional dissection of KorA, we used random mutagenesis and a positive selection system to id

27 We applied PCR-driven random mutagenesis and a retrovirus-mediated expression

28 nd how E1 interacts with origin DNA, we used random mutagenesis and a yeast one-hybrid screen to sele

29 We used both random mutagenesis and alanine scanning to identify alph

30 We have used random mutagenesis and alanine scanning to identify dete

31 Using random mutagenesis and an anaerobic metabolic selection,

32 This study demonstrated that random mutagenesis and bioselection procedures could be

33 A series of adiC mutants isolated by random mutagenesis and by targeted deletion were shown t

34 We conclude that semi-random mutagenesis and directed evolution will play a pr

35 Four rounds of random mutagenesis and DNA shuffling of Drosophila melan

36 Here, random mutagenesis and DNA shuffling of the single-chain

37 However, random mutagenesis and expression of the maize (Zea mays

38 Molecular dynamics simulations, random mutagenesis and flash photolysis studies indicate

39 s then mutagenized, and successive rounds of random mutagenesis and flow cytometry selection are done

40 east, the affinity was matured by sequential random mutagenesis and fluorescence-activated cell sorti

41 is, a mariner-based transposon, was used for random mutagenesis and for the isolation of 10,325 S. au

42 We applied random mutagenesis and generated recombinant influenza v

43 tuent amino acids Val(700)-Arg(712) by using random mutagenesis and genetic selection.

44 ised activity and was therefore subjected to random mutagenesis and genetic selection.

45 Using a random mutagenesis and growth selection approach, we ide

46 Through random mutagenesis and growth selection, we have generat

47 A combination of random mutagenesis and high-throughput screening was use

48 Directed evolution techniques (random mutagenesis and high-throughput screening) offer

49 he mutations were previously generated using random mutagenesis and identified by high-temperature sc

50 prove affinity by using a combined method of random mutagenesis and in silico assisted design to affi

51 In addition, random mutagenesis and in vitro evolution are being cons

52 In this study we used random mutagenesis and intragenic pseudoreversion analys

53 nal importance in CheZ, we subjected cheZ to random mutagenesis and isolated 107 nonchemotactic CheZ

54 Thus, random mutagenesis and mutational analysis allows for th

55 specify unique tertiary folds, we have used random mutagenesis and phage display selection to evolve

56 nt scDR1alphabeta molecules was generated by random mutagenesis and screened by fluorescence activate

57 21 amino acids (Leu228-Leu248) to intensive random mutagenesis and screened multiply substituted all

58 scherichia coli rpsL gene, encoding S12, for random mutagenesis and screened the resulting mutants fo

59 Eleven rounds of random mutagenesis and screening via fluorescence-activa

60 Three consecutive rounds of random mutagenesis and screening were performed and yiel

61 ven particularly amenable to enhancement via random mutagenesis and screening, yet the effects of mos

62 in of the beta3 integrin subunit to unbiased random mutagenesis and selected it for activated mutants

63 y in the tryptophan screen were subjected to random mutagenesis and selection by complementation.

64 Here, we describe an expanded random mutagenesis and selection experiment that yielded

65 Nontoxic PAP mutants have been isolated by random mutagenesis and selection in yeast.

66 Random mutagenesis and selection using phage or cell sur

67 previously phenotypically selected following random mutagenesis and shown to lie in the actin binding

68 We have used random mutagenesis and site-directed mutagenesis to iden

69 Using a combination of in silico analysis, random mutagenesis and site-directed mutagenesis, we ide

70 ally, we subjected the o2 loop of the M3R to random mutagenesis and subsequently applied a novel yeas

71 Random mutagenesis and suppressor selection in a Rubisco

72 the gene expression phase can be changed by random mutagenesis and that a single-nucleotide substitu

73 In this study we used random mutagenesis and the mammalian two-hybrid method M

74 net result of four sequential generations of random mutagenesis and the one recombination step is a 5

75 tic analysis of SSV1 using both specific and random mutagenesis and thereby generate mutations in all

76 imited by the inefficiencies of conventional random mutagenesis and transgenesis.

77 the adhesive subunit, draE, was subjected to random mutagenesis and used to complement a strain defec

78 agment (residues 273-621) was constructed by random mutagenesis and was screened for reduced binding

79 mutations, the F155V mutant was subjected to random mutagenesis and was used as a parent for the isol

80 ghlight the usefulness of combining receptor random mutagenesis and yeast expression technology to st

81 Using random mutagenesis and yeast surface display, we defined

82 tor receptor (EGFR) was accomplished through random mutagenesis and yeast surface display.

83 lactone synthesis by RhiI were identified by random mutagenesis, and all mapped to a conserved region

84 lication of rational or semirational design, random mutagenesis, and biochemical characterization.

85 To date, only random mutagenesis, and not directed gene knockouts, hav

86 Site-directed mutagenesis, random mutagenesis, and site-saturation mutagenesis were

87 e-DNA interactions, PCR-based codon-specific random mutagenesis, and site-specific mutagenesis were p

88 ansporter (SERT) was previously subjected to random mutagenesis, and the mutation V382P was found to

89 the LTR-retrotransposon Tf1 was subjected to random mutagenesis, and the resulting transposons were s

90 Here we used a random mutagenesis approach and selected TBZ-resistant m

91 We applied a random mutagenesis approach and steady-state kinetic ana

92 We have employed a random mutagenesis approach to identify further amino ac

93 We employed a random mutagenesis approach to identify novel monogenic

94 Here we have taken a random mutagenesis approach to identify substitutions in

95 In the current study, a random mutagenesis approach was used to create a mutant

96 Thus, by using a random mutagenesis approach, we have shown for the first

97 With a transposon-based, random mutagenesis approach, we identified potential LSR

98 Using domain swapping, site-directed, and random mutagenesis approaches, we here show that residue

99 7 helicase-primase protein (4A') prepared by random mutagenesis as reported in the accompanying paper

100 d broadly diversify populations of clones by random mutagenesis as well as homologous recombination-d

101 ncies of several other techniques: recursive random mutagenesis (asexual), combinatorial cassette mut

102 Random mutagenesis at positions 124 and 128 revealed tha

103 of the two transmembrane helices followed by random mutagenesis at targeted residues.

104 owards engineering o-ribosomes have involved random mutagenesis-based approaches.

105 Using a high-throughput, random mutagenesis-based variomics screen, we generated

106 olved including advances using gene-specific random mutagenesis by dNTP analogs that identify key res

107 Random mutagenesis by error-prone polymerase chain react

108 Random mutagenesis by hydroxylamine treatment and error-

109 d host range vector, pGK12, and subjected to random mutagenesis by passage through Epicurian coli mut

110 Random mutagenesis constitutes an important approach for

111 topology, and orientation of F1 by employing random mutagenesis, cysteine scanning, and disulfide cro

112 ich contains the catalytic region defined by random mutagenesis, did not bind DNA and was a mixture o

113 Hence, random mutagenesis discovered two new residues, G407 and

114 rate selectivities of enzymes resulting from random mutagenesis, DNA shuffling, and combinatorial sat

115 ot- mutations of fliG isolated previously by random mutagenesis do not alter the putative active-site

116 the novel technique of disulfide trapping by random mutagenesis (DTRM) and molecular modeling.

117 programmed mutagenesis in antibody genes to random mutagenesis during species evolution or developme

118 lic engineering with phage genes followed by random mutagenesis enabled us to achieve approximately 7

119 Together with random mutagenesis experiments and interaction measureme

120 Furthermore, site-directed and random mutagenesis experiments showed that point mutatio

121 Random mutagenesis followed by a filter-based screening

122 By using receptor random mutagenesis followed by a genetic screen in yeast

123 the D113N mutant M(3) receptor to PCR-based random mutagenesis followed by a yeast genetic screen to

124 been learned about vertebrate development by random mutagenesis followed by phenotypic screening and

125 We used the technique of random mutagenesis followed by positive genetic selectio

126 A single round of random mutagenesis followed by recombination of improved

127 Random mutagenesis followed by screening for separation

128 This strategy of random mutagenesis followed by selection for surface exp

129 ted an allelic series of Cry mutants through random mutagenesis, followed by a cell-based screen to i

130 rotein as a model system and subjected it to random mutagenesis, followed by screening for variants w

131 reen for active Kir3.2 channels subjected to random mutagenesis has identified residues in the transm

132 Random mutagenesis has revealed that most substitutions

133 gene manipulation in mice (e.g., targeted or random mutagenesis) has been accompanied by increased re

134 Using several point mutations isolated by random mutagenesis, here we studied the role of 25S rRNA

135 Random mutagenesis identified a number of additional mut

136 Random mutagenesis identified two different mutations th

137 Random mutagenesis identifies numerous YF1 variants in w

138 Previously, we performed random mutagenesis in a DeltaveA strain and identified r

139 Methods to enhance random mutagenesis in cells offer advantages over in vit

140 oad substrate specificity using rational and random mutagenesis in combination with in vitro activity

141 We used random mutagenesis in combination with the yeast two-hyb

142 rative directed evolution approach employing random mutagenesis in conjunction with homologous recomb

143 used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells.

144 We present a system for random mutagenesis in Escherichia coli for the evolution

145 n enhanced retroviral mutagen (ERM)-mediated random mutagenesis in the estrogen-dependent T47D breast

146 studying a single biological process through random mutagenesis in the mouse, we have identified rece

147 If so, a low level of random mutagenesis in the vicinity of the chimera bindin

148 eficient (R-) mutations in a second round of random mutagenesis in this mutant background.

149 is study we subjected the cloned cpe gene to random mutagenesis in XL-1 Red strains of Escherichia co

150 Random mutagenesis is an approach that has the potential

151 In polyploid species, altering a trait by random mutagenesis is highly inefficient due to gene red

152 assay for intimin-Tir interaction and, after random mutagenesis, isolated 24 point mutants in intimin

153 es continue to limit routine construction of random mutagenesis libraries in Escherichia coli, in par

154 izing anti-gp120 antibody VRC01 using both a random mutagenesis library and a defined mutant panel an

155 ttempt to solve this problem, we subjected a random mutagenesis library of the scFv X5 to sequential

156 The program can mimic almost any type of random mutagenesis library, including those produced via

157 Using random mutagenesis, many mutations of cytoplasmic residu

158 Random mutagenesis methods only partially cover the muta

159 library of Cre variants produced by targeted random mutagenesis must rapidly catalyze recombination,

160 perature-sensitive mutants were isolated via random mutagenesis of 13 carboxyl-terminal residues.

161 v-hFc, from a library of mutants obtained by random mutagenesis of a pool of hFc binders.

162 n principle, this approach may be applied to random mutagenesis of any enzyme that converts chemicals

163 We performed random mutagenesis of CDC28 to identify additional allel

164 CAT-2200 from a parental lead antibody using random mutagenesis of CDR3 loops.

165 We describe a general method for random mutagenesis of cloned genes by error-prone PCR or

166 isolated SpoIVB-resistant SpoIIQ proteins by random mutagenesis of codons at the cleavage site and de

167 Following random mutagenesis of dctD, 55 independent mutant forms

168 By random mutagenesis of eight active site residues, we iso

169 We used random mutagenesis of histones H3 and H4 followed by a r

170 Random mutagenesis of human DNA topoisomerase IIbeta cDN

171 Random mutagenesis of human papillomavirus type 16 (HPV1

172 ing the framework of humanized antibodies by random mutagenesis of important framework residues.

173 Directed, random mutagenesis of invariant residues proline 76 and

174 rated by light-chain shuffling combined with random mutagenesis of its heavy-chain variable domain an

175 Through random mutagenesis of one such hybrid MTS-COX2, we ident

176 ymerase, we carried out alanine scanning and random mutagenesis of oxyR.

177 roved 4.8-fold to nearly wild-type levels by random mutagenesis of purF and genetic selection.

178 These objectives were accomplished by random mutagenesis of RAP, which identified two critical

179 nts of this receptor, we conducted extensive random mutagenesis of receptors followed by screening fo

180 ly found to improve affinity at site 1 or by random mutagenesis of residues in site 2 followed by pha

181 Here we report that random mutagenesis of residues selected based on alignme

182 Random mutagenesis of RMC26 produced defects in the sorb

183 the roles of sigma(54) in initiation we used random mutagenesis of rpoN and an in vivo activity scree

184 Random mutagenesis of rsbP yielded 17 independent bypass

185 However, following random mutagenesis of Rx, we identified mutants in which

186 We present a procedure for efficient random mutagenesis of selected genes in a bacterial chro

187 Random mutagenesis of SH3 identified several novel point

188 ntify the significant amino acids in loop D, random mutagenesis of six highly conserved loop residues

189 aratus and for its function in colonization, random mutagenesis of tcpF was performed.

190 Random mutagenesis of the 412-bp eis promoter, using a c

191 the function of the Air1/2 protein, we used random mutagenesis of the AIR1/2 gene to identify residu

192 Random mutagenesis of the Asp2 region containing this ca

193 for transcriptional activation, an extensive random mutagenesis of the C1 carboxyl terminus was done.

194 After random mutagenesis of the chloroplast petD gene, coding

195 regation and in transfer, we first performed random mutagenesis of the cluA gene using a modified min

196 Following random mutagenesis of the Eco RV endonuclease, a high pr

197 This approach involves low-frequency random mutagenesis of the entire M3R coding sequence, fo

198 Random mutagenesis of the feuPQ bicistron revealed a def

199 In this study, we conducted a random mutagenesis of the hATR coding sequence.

200 Random mutagenesis of the inactive E96A mutant cDNA, fol

201 em was validated for the engineered mutants, random mutagenesis of the intron followed by genetic and

202 library of human ERalpha mutants created by random mutagenesis of the ligand binding domain of human

203 Random mutagenesis of the mechanosensitive channel of la

204 t of broad-spectrum resistance, we performed random mutagenesis of the N-terminal domains of this bro

205 Here we performed random mutagenesis of the RNA-guided Cas9 nuclease to lo

206 Random mutagenesis of the syringafactin-deficient strain

207 gnal transduction through cAR1, we performed random mutagenesis of the third intracellular loop (24 a

208 Through random mutagenesis of this globular tail, we isolated si

209 ding to FcRn at pH 6.0 has been increased by random mutagenesis of Thr252, Thr254, and Thr256 followe

210 ertion and toxicity, either site-directed or random mutagenesis of two regions was performed.

211 in FtsQ critical for function, we performed random mutagenesis on the ftsQ gene and identified four

212 nging the pattern of gene expression through random mutagenesis or by introducing expression librarie

213 onic drug/>/= 2 proton-antiporter, either by random mutagenesis or by rational design.

214 ey have not been identified as beneficial by random mutagenesis or DNA shuffling or seen in any of th

215 ine substitution based on the results of the random mutagenesis or on their homology to residues that

216 ts have traditionally been obtained by using random mutagenesis or retroviral insertions, methods tha

217 rried out polymerase chain reaction-mediated random mutagenesis over a large part of the cAR1 sequenc

218 In principle, random mutagenesis procedures such as phage display coul

219 Recursive random mutagenesis produced essentially asexual populati

220 le nucleotide polymorphism (SNP) studies and random mutagenesis projects identify amino acid substitu

221 tide polymorphism (SNP) data and large-scale random mutagenesis projects.

222 c stem (ES) cells on a large scale by tagged random mutagenesis provides a powerful approach for dete

223 utations of the D2s receptor were created by random mutagenesis (R233G and A234T).

224 Six generations of random mutagenesis, recombination, and screening stabili

225 (6) variants were screened in four rounds of random mutagenesis, resulting in two hERalphaLBD variant

226 Based upon the random mutagenesis results, we performed an alanine scan

227 hermore, mutants of AtMTP1 generated through random mutagenesis revealed residues embedded within tra

228 Random mutagenesis revealed that P185Q mutation in the T

229 A combined strategy of random mutagenesis, saturation mutagenesis and in vitro

230 We used random mutagenesis, saturation mutagenesis, and screenin

231 For less well-characterized genes, a novel random mutagenesis scheme was developed that allows a co

232 can be identified among progeny of mice in a random mutagenesis screen (phenotype-based mutagenesis o

233 bility gene 101 (Tsg101) was identified in a random mutagenesis screen for potential tumor suppressor

234 ng of resistant clones generated from a MEK1 random mutagenesis screen in vitro, as well as tumors ob

235 sis of this interaction, we have developed a random mutagenesis screen in yeast approach for efficien

236 A random mutagenesis screen led to the isolation of mutati

237 We carried out a cell-based, in vitro random mutagenesis screen to identify EGFR mutations tha

238 To uncover novel domains, a random mutagenesis screen using an M6 Mga (mga-1) was un

239 Using a random mutagenesis screen, we identified multiple point

240 ntified, confirming the completeness of this random mutagenesis screen.

241 the identification of important residues by random mutagenesis screening techniques, as well as in t

242 Gly(22) was highlighted in random mutagenesis screens of E. coli MscL.

243 To gain insights herein, we conducted random mutagenesis screens to identify B- or C-RAF mutat

244 rected evolution campaign with two rounds of random mutagenesis (SeSaM followed by epPCR), site satur

245 Numerous methods, including random mutagenesis, site-directed mutagenesis and DNA sh

246 In this study, random mutagenesis strategies were used to define residu

247 In this study we employed a random mutagenesis strategy to construct a library of po

248 be induced by creating point mutations via a random mutagenesis strategy.

249 Previous random mutagenesis studies implicated residues in the re

250 A previous random mutagenesis study identified a W165Y/E166Y/P167G

251 A truncation mutant (K360stop) identified by random mutagenesis suggested a small region near the C t

252 n important step in the development of a new random mutagenesis system.

253 We performed random mutagenesis targeting residue W1065 of GTF180-Del

254 Through use of a combinatorial random mutagenesis technique (DNA shuffling), we have is

255 Albino mutants derived from random mutagenesis techniques may contain mutations in g

256 We used random mutagenesis techniques to create a plasmid librar

257 ions that are unattainable using traditional random mutagenesis techniques, this approach will be val

258 Here, we used random mutagenesis to construct a library of lysis-defec

259 genetic screen consisted of four steps: (1) random mutagenesis to create a plasmid library, each bea

260 importance of the domains of Snu114, we used random mutagenesis to create conditionally lethal allele

261 esign of the active-site binding pockets and random mutagenesis to create protein variants that are o

262 First, we used random mutagenesis to create temperature-sensitive allel

263 in interactions, we used receptor saturation random mutagenesis to generate a yeast library expressin

264 We used retrovirus insertion-mediated random mutagenesis to generate tumor necrosis factor (TN

265 sed the yeast two-hybrid system coupled with random mutagenesis to identify a mutant mSIN1 (mSIN1/Q68

266 I-1 to the latent conformation, we have used random mutagenesis to identify the 33B8 epitope in PAI-1

267 oorganisms, application of site-directed and random mutagenesis to increase the substrate range or ac

268 We used random mutagenesis to isolate RhaS and RhaR variants wit

269 nomena at the level of the receptor, we used random mutagenesis to isolate variants of Staphylococcus

270 p40 with the regulatory domain was mapped by random mutagenesis to same surface of the alpha-helix th

271 ersiniabactin assembly lines were evolved by random mutagenesis to support growth under selection con

272 We used random mutagenesis to uncover recombination-deficient mi

273 aracterize the DM motif by site-directed and random mutagenesis using a yeast one-hybrid (Y1H) system

274 wn-regulatory mutant (E38A) was subjected to random mutagenesis using error-prone polymerase chain re

275 The results from random mutagenesis using the rapid three-hybrid binding

276 In the current study, PCR-based random mutagenesis was employed to generate unbiased los

277 Random mutagenesis was employed to probe for amino acid

278 (i) Yeast one-hybrid-based random mutagenesis was first used to identify a number o

279 ting of the Golgi isoform of the V-ATPase, a random mutagenesis was performed on the N terminus of St

280 specific FhbA residues involved in binding, random mutagenesis was performed.

281 nd largely uncharacterized regulator family, random mutagenesis was used to gain insights into how th

282 A combination of deletion analysis and random mutagenesis was used to identify regulatory eleme

283 By random mutagenesis we isolated 13 different single amino

284 omes the barrier that had prevented L. casei random mutagenesis, we developed a signature-tagged muta

285 Gathering information from random mutagenesis, we further recombined the beneficial

286 Using random mutagenesis, we generated six loss-of-function po

287 Using both rational and random mutagenesis, we have created the first known broa

288 Using a combination of rational and random mutagenesis, we have engineered IDH to use isopro

289 By random mutagenesis, we identified a mutant RhlB(P238L) t

290 rotein templates followed with site-targeted random mutagenesis, we identified a selective peroxynitr

291 Following random mutagenesis, we isolated eight mutant forms of Sa

292 After a random mutagenesis, we isolated TRPY1 mutants that respo

293 By random mutagenesis, we switched the specificity of the Y

294 Two generations of random mutagenesis were performed, from which three posi

295 role of IpaD during activation, we combined random mutagenesis with a genetic screen to identify ipa

296 This strategy combines random mutagenesis with a switch-like reporter of transc

297 Random mutagenesis with ouabain selection has been used

298 Protein sequences evolve through random mutagenesis with selection for optimal fitness.

299 This approach, which combines saturating random mutagenesis with targeted selection of mutations

300 Random mutagenesis within the context of the AdV genome