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1 ic analysis of microtubule pull-downs, using ratiometric (14)N/(15)N labeling, revealed 605 proteins
5 tion in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPP
12 dentify the regimes in which the widely used ratiometric analysis of signals is substantially subopti
13 thrombin as a model protease, we show that a ratiometric analysis of the emission from the QD, Cy3, a
18 UV lamp was used as an excitation source and ratiometric analysis using an iPad camera was possible b
19 ended to overcome this technical limitation: ratiometric analysis using monochromatic, evanescent dar
21 The studies showed that 4-MB exhibits a dual ratiometric and calorimetric response toward peroxynitri
24 fluorescence lifetime spectroscopy with the ratiometric approach and investigate potential non-FRET
28 potential, monitored by the dual wavelength ratiometric approach utilizing the probe di-8-ANEPPS.
32 mutant to increase the ECV pH in the in vivo ratiometric assay suggests that ammonia produced by the
34 ave developed a new RNA imaging probe called ratiometric bimolecular beacon (RBMB), which combines fu
35 we developed an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), which generates a
36 uitin assays, they were recovered in vivo by ratiometric bimolecular fluorescence complementation, an
38 ured by X-rhod-1 or mitochondrially-targeted ratiometric biosensor 4mtD3cpv, or ADP-ATP exchange rate
42 xibility in the design of a wide spectrum of ratiometric biosensors and expanded in vivo applications
43 truct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspas
44 a genetically encoded Ca(++) sensor that is ratiometric by virtue of bioluminescence resonance energ
46 ponse of a low-affinity, genetically encoded ratiometric Ca(2)(+) indicator (D4cpv) calibrated agains
55 single-emission biosensors into a series of ratiometric calcium sensors (MatryoshCaMP6s) and ammoniu
56 nt concentration, researchers have developed ratiometric CEST imaging, which investigates contrast ag
59 The derivative L1 detects Zn(2+) by bringing ratiometric change in the fluorescence signals at 390 an
60 GTP binding to these sensors results in a ratiometric change in their fluorescence, thereby provid
63 ctured fluorophores as they show significant ratiometric changes in emission upon metal complexation;
64 s(II) and Ag(I) complexes exhibit remarkable ratiometric changes of intensity for phosphorescence ver
65 plex is reported for imaging pH by MRI using ratiometric chemical exchange saturation transfer (CEST)
69 ct quantity versus the donor, we utilize the ratiometric comparison of the two to obtain the stoichio
70 otein phosphorylation with high precision by ratiometric comparisons using heavy and light thiocholin
71 ostic product ions during SRM transitions by ratiometric comparisons with stable isotope internal sta
72 individual protomers in complexes where the ratiometric composition of LHR(B-) to LHR(S-) modulated
73 report the synthesis of a new water-soluble ratiometric Cu(II) dye with a moderate affinity (10(9) M
74 was successfully constructed via fluorescent ratiometric detection and fluorescence lifetime imaging;
75 ng capability in a single vector with robust ratiometric detection by exploiting a network of FRET pa
78 he application of our sensing system for the ratiometric detection of Mg(2+) in target organelles in
79 rescent probes that have been applied to the ratiometric detection of various analytes, including cat
80 in the presence of L-cysteine is slow, but a ratiometric detection process in the therapeutic window
83 e fragmentation strategy that allows for the ratiometric determination of relative H2S and cysteine (
87 e, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STE
88 ncy, and discriminate collagen and myosin by ratiometric epi-generated SHG images at 920 nm and 860 n
91 odulate the intensity of such phenomena in a ratiometric fashion, in direct proportion with analyte c
94 mine fluorescence intensities functions as a ratiometric fluorescence chemodosimeter for mercury.
95 IP-1), a reactivity-based probe that enables ratiometric fluorescence imaging of labile iron pools in
98 H of individual lysosomes using quantitative ratiometric fluorescence microscopy and report an unappr
100 al sensing methods currently being explored, ratiometric fluorescence sensing has received particular
101 The o-amino GFPSCs can serve as wavelength-ratiometric fluorescence sensors that selectively recogn
102 r detection of hydroxyl radical based on the ratiometric fluorescence signal between 7-hydroxy coumar
104 a convenient strategy to design an efficient ratiometric fluorescent bioimaging probe for metal ions.
106 studies, it can be used to develop thin-film ratiometric fluorescent chemosensors with improved optic
107 e proof of concept that the colorimetric and ratiometric fluorescent cyanide-selective chemodosimeter
109 and dynamics of LH using genetically encoded ratiometric fluorescent heme sensors in the unicellular
110 This feature was utilized for the real-time ratiometric fluorescent imaging of MsrA activity in E. c
111 H probes for use in live-cell STED nanoscopy.Ratiometric fluorescent pH probes are useful tools to mo
115 signed and synthesized as a colorimetric and ratiometric fluorescent probe that can be utilized to ra
116 e report the design and application of a new ratiometric fluorescent probe, which contains different-
117 er, and then polarized, labeled with JC-1 (a ratiometric fluorescent probe, which indicates changes i
118 ard the design strategy of a new category of ratiometric fluorescent probes facilitating precise and
119 ETHODS AND We used a novel, redox-sensitive, ratiometric fluorescent protein sensor (RoGFP) to assess
120 Keima is a pH-sensitive, dual-excitation ratiometric fluorescent protein that also exhibits resis
124 Using this design principle, we generate a ratiometric fluorescent sensor protein for methotrexate
125 acid technology permits the semisynthesis of ratiometric fluorescent sensor proteins with unprecedent
128 vely termed as DC-SIGN/R) using a sensitive, ratiometric Forster resonance energy transfer (FRET) ass
129 as donor showed the highest dynamic range in ratiometric FRET imaging experiments with the G-protein
133 mouse, Arl13b-mCherry-GECO1.2, expressing a ratiometric genetically encoded calcium indicator in all
137 features make it particularly favorable for ratiometric Hg(2+) sensing and bioimaging applications.
143 ral confocal microscopy approach to generate ratiometric images of the plasma membrane surface of Bri
144 large dynamic range of the construct enabled ratiometric imaging (with Rox excitation) of caspase act
148 t insensitive to Cl(-) and pH), we show that ratiometric imaging is strongly affected by the optical
149 shifted TagRFP/mPlum FRET pair with spectral ratiometric imaging of an ECFP/Venus pair we were thus a
150 We show the use of these BRET systems for ratiometric imaging of both cells in culture and deep-ti
151 Transgenic expression of Salsa6f enables ratiometric imaging of Ca(2+) signals in complex tissue
159 o correlate microtubule and ER behavior, and ratiometric imaging was used to assess intracellular Ca(
160 d in Arabidopsis (Arabidopsis thaliana), and ratiometric imaging was used to monitor cytosolic Pi dyn
162 que combination of features: dual-excitation ratiometric imaging, high dynamic range, good signal-to-
163 of labile Cu(+) pools within living cells by ratiometric imaging, including expansion of endogenous s
170 RACT: The calcium-modulated photoactivatable ratiometric integrator (CaMPARI) is a genetically encode
172 ference for fluorescent indicators to enable ratiometric intensity or dually lifetime referenced meas
173 ere employed as temperature indicators using ratiometric intensity-based fluorescence techniques.
174 ally encoded tag for electron microscopy and ratiometric light microscopy, we go on to show that bulk
175 lipid identification was carried out using a ratiometric lipid standard on a TSQ Quantum Access Max m
176 g, pulse-chase analysis, and high-resolution ratiometric live-cell studies demonstrate that mDia2 and
179 ells, and channel activity was determined by ratiometric measurement of [Ca (2+)] i in response to cy
182 ator performance such as Kd, wavelength, and ratiometric measurements, the use of NP sensors can achi
186 as off-target binding or uneven delivery, a ratiometric method is employed to quantify the specific
188 Inspired by dual-wavelength fluorescence ratiometric method which could reduce the influence from
189 robust sensor for pH determination using the ratiometric methodology where excitation at a single wav
190 ple targets as well as for implementation of ratiometric methods that can improve accuracy and precis
195 gel transitions, simple changes in color, or ratiometric monitoring of the differences in the fluores
197 new pathway to design ultrasmall fluorescent ratiometric nanoindicators with tunable wavelengths and
199 cantly increased signal change compared with ratiometric NES-YC3.6 in response to several stimuli.
202 gnition), halogenated hydroxy-coumarin core (ratiometric optical pH sensing in the desired pH range),
204 support of multiplexing and error correcting ratiometric or differential measurement approaches.
205 trate that REX-GECO1 can be used as either a ratiometric or intensiometric Ca(2+) indicator in organo
207 pH (pHm) and DeltaPsim were monitored using ratiometric pericam and tetramethylrhodamine ethyl ester
210 luorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization ser
215 NP by fine-tuning of the surface coverage; a ratiometric pH response was then observed owing to stron
220 ystem lumen, we targeted genetically encoded ratiometric pH sensors to the cytosol, the endoplasmic r
223 se in vacuolar and cytosolic pH homeostasis, ratiometric pH-sensitive fluorophores specific for the v
225 998, genetically encoded pH-sensitive sensor ratiometric pHluorin proved to be a valuable tool for ce
226 , we designed APC-1 and APC-2 explicitly for ratiometric photoacoustic imaging by using an aza-BODIPY
228 g the development of the first near-infrared ratiometric photoacoustic probe for in vivo real-time im
231 With respect to single-emission probes, this ratiometric probe has demonstrated to feature excellent
233 is demonstrated utilizing four known optical ratiometric probes and sensors, together with a discussi
234 escribe a general platform for the design of ratiometric probes based on a heterometallic Tb(3+)/Eu(3
238 entified subpopulations of DCIS lesions with ratiometric properties resembling either benign fibroade
239 es on photodissociation of cut s10 from GFP, ratiometric protease sensors were designed from the cons
241 Lipids were identified and subjected to ratiometric quantification using a TSQ quantum Access Ma
242 gnetic resonance (MR) probe that enables the ratiometric quantitation and imaging of pH through chemi
246 ication ratio control system' (ARCS) permits ratiometric representation of amplicons relative to the
248 ts in combination with the recently reported ratiometric resonance synchronous spectroscopic (R2S2, p
250 achieves this with a 2.4% change/ degrees C ratiometric response over physiological temperatures in
252 w, excellent photostability, self-referenced ratiometric response, fast response, and high selectivit
253 orm (low concentration range), it displays a ratiometric response, while a "turn-off" sensing is obse
257 vised sensor film can be used for referenced ratiometric sensing and 2D imaging of pH using a color c
258 uses only a single emitter, which simplifies ratiometric sensing and broadens the applications of the
260 BSA-AuNCs offered a sensitive and reversible ratiometric sensing of a 0.1-pH unit change in the pH ra
262 of a dual emission probe for the fluorescent ratiometric sensing of hydrogen peroxide (H2O2), enzyme
263 sion properties could serve as the basis for ratiometric sensing of the cellular receptor's local che
265 e technique, the so called lifetime assisted ratiometric sensing to discriminate the two fluorescence
266 ecific repertoire of computations, including ratiometric sensing, balance detection, and imbalance de
267 nsitive phosphorescence or dual emission for ratiometric sensing, often by blending two dyes in a mat
270 sion enables the development of the CD-based ratiometric sensor for Fe(III) ions and pyrophosphate ba
274 them ideal candidates for the preparation of ratiometric sensors based on absorption-based indicators
279 ports investigation of the dependence of the ratiometric signal on the type of linkage used to conjug
280 lls the sensors showed consistent changes in ratiometric signal upon depletion or restoration of GTP
283 nanosized optical pH probes need to provide ratiometric signals in the optically transparent biologi
284 yield light-up signals of up to 40-fold, or ratiometric signals with ratio changes of 82-fold, on en
287 e combine APEX technology with a SILAC-based ratiometric tagging strategy to substantially reduce unw
288 mportantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelengt
289 on describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting
290 4] is an unprecedented case of a luminescent ratiometric thermometer based on a very stable silicate
291 of open cellular regions, we instead use a 'ratiometric' three-state SILAC protocol for high spatial
292 f QD-FRET methods with digital imaging for a ratiometric transduction of nucleic acid hybridization o
293 palette of a digital camera for quantitative ratiometric transduction of nucleic acid hybridization o
297 stablish NC-Os(II)PP conjugates as competent ratiometric, two-photon oxygen sensors for application i
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