ライフサイエンスコーパス: read through

1 r therapeutics: exon skipping and stop codon read through.

2 ic and bacterial cells results in stop-codon read through.

3 ame extent as the effects on [PSI+]-mediated read-through.

4 depletion of TIA-1 by siRNA increased (pA)p read-through.

5 82X mutation, CFTR1281, without the need for read-through.

6 e ade1-14 selective marker for translational read-through.

7 of the [PSI+] prion to cause nonsense-codon read-through.

8 (+)] prion state leads to more translational read-through.

9 minimal sequence necessary for translational read-through.

10 st increase in frame shifting and stop codon read-through.

11 e expression and drugs for nonsense mutation read-through.

12 ras are functional outcomes of transcription read-through.

13 vation as a potent enhancer of transcription read-through.

14 operties of reverse transcriptase, which can read through 2',5'- or 2',3'-branches and efficiently pe

15 f the 91 unique viral sequences annotated as read-through, 90% had one of six of the 64 possible codo

16 y assessing the ability of RNA polymerase to read through a factor-dependent terminator.

17 transcribed the antitermination sequence to read through a Rho-dependent terminator.

18 construction of highly accurate preassembled reads through a directed acyclic graph-based consensus p

19 dependent transcription of the tcpA promoter reads through a proposed terminator between the tcpF and

20 requires special translational cofactors to "read-through" a UGA-stop codon that specifies SEC incorp

21 compound RTC14 did not result in significant read-through activity in vivo and demonstrated the level

22 ough, suggesting that these factors modulate read-through activity.

23 osome-binding antibiotic with translational 'read-through' activity, efficiently targeted abnormal WN

24 with the classical aminoglycoside antibiotic read-through agent geneticin (G418) across a diverse ran

25 le PTC124 (Ataluren) has been described as a read-through agent, capable of suppressing premature ter

26 rmination factor, which increases stop codon read-through allowing ribosomes to translate into the 3-

27 5.8 kb) arising from incomplete splicing and reading through an intron.

28 transcriptional interference resulting from read through and dual strand transcription.

29 highlight a novel link between transcription read-through and aberrant expression of oncogenes and ch

30 3' end of exon 14, leading to the subsequent read-through and capture of formerly intronic sequence a

31 nzyme can respond to SII for transcriptional read-through and carry out SII-activated nascent RNA cle

32 ly extended proteins and elevated stop codon read-through and frameshift events.

33 ngle-component RNA viruses use translational read-through and frameshift mechanisms to down-regulate

34 Overexpression of Sup35 decreases stop codon read-through and rescues oxidant tolerance consistent wi

35 f-of-concept study to demonstrate successful read-through and restoration of RP2 function for the R12

36 elation between codon-specific translational read-through and the activity of a 120-kDa RNA-binding p

37 dicated small amounts of native ATP7A(R201X) read-through and were associated with a dramatic clinica

38 to overcome RDEB PTC mutations by inducing "read-through" and incorporation of an amino acid at the

39 g the effects of put mutations on terminator read-through, and by probing wild-type and mutant put RN

40 en reading frames, extensive transcriptional read-through, and numerous unpredicted RNA start sites h

41 econdary structures may cause frameshifting, read-through, and/or recoding of the multiple stop codon

42 heterochromatin and RNAi factors to suppress read-through antisense transcripts.

43 hile G418 exhibits varying activity in every read-through assay, there is no evidence of activity for

44 Using a sensitive terminator read-through assay, we identified trans-acting Terminato

45 rf virus (PAV serotype) coat protein gene is read through at a low rate.

46 It retarded termination and increased read-through at Rho-dependent terminators, even in the a

47 The [PSI+] state causes read-through at stop codons and can lead to phenotypic v

48 nome Atlas (TCGA) reveals that transcription read-through beyond the termination site is a source of

49 on factor TFIIS enables RNA polymerase II to read through blocks to elongation in vitro and interacts

50 strongly inhibit [PSI+]-mediated stop codon read-through but do not cure cells of the [PSI+] prion.

51 ted region of the luciferase gene stimulated read-through by 6-11-fold in selenium-replete cells; abs

52 Promotion of read-through by MoMLV RNase H prevents nonsense-mediated

53 wo mRNAs are linked due to a small amount of read-through by RNA polymerase.

54 on elongation complexes and facilitate pause read-through by stabilizing RNAP in a posttranslocated r

55 ocess of nonsense suppression (translational read-through) by ribosomes, making it difficult to deter

56 ability of hnRNP H mutations to suppress the read-through caused by an SR protein mutation suggests t

57 The ability of the cellular machinery to read through click-linked DNA was further probed by usin

58 NMD also limits the efficacy of read-through compound (RTC)-based therapies.

59 We also show how the read-through compound ataluren (PTC124) increases PPT1 e

60 ent stem cells, and show that small molecule read-through compounds, designed to induce read-through

61 Two such read-through compounds, RTC13 and RTC14, were recently i

62 The relative efficiency of these read-through contexts in mammalian tissue culture cells

63 lls lacking RP2 indicates that translational read-through could be clinically beneficial for patients

64 ing replication from base mismatches and can read through damaged bases, such as abasic sites and thy

65 n which the maximal efficiency of stop codon read-through depends on the interaction between MoMLV RT

66 The degree of read-through-derived peptide presentation varies with th

67 alyses suggest that structure throughout the read-through domain affects the regulation of viral repl

68 ng shows that structural features within the read-through domain of delta 159 RNA are less well deter

69 e RNA secondary structure is restored in the read-through domain of delta 549 RNA.

70 type RNA, whereas predicted structure in the read-through domain of evolved pseudorevertant delta 549

71 changes in structural plasticity within the read-through domain of the mutant genomes are key in und

72 re less well determined than they are in the read-through domain of wild-type RNA, whereas predicted

73 (delta 159 and delta 549) located within the read-through domain, a 850 nucleotide hairpin, in coliph

74 everal long range helices at the base of the read-through domain, that suppress translational initiat

75 scription elongation complex is competent to read through downstream termination signals.

76 h NUT RNA, which, in turn, causes failure to read through downstream termination sites.

77 loop termed boxB, enabling RNA polymerase to read through downstream terminators processively.

78 acologic NMD inhibition combined with a PTC "read-through" drug led to restoration of full-length p53

79 Treatment with read-through drugs also leads to increased protein funct

80 We have also shown how the read-through drugs gentamicin and ataluren (PTC124) incr

81 phin are specifically targeted by stop codon read-through drugs, whereas out-of-frame deletions and i

82 The amber codon is thus read through during translation at apparently high effic

83 ontain an in-frame amber (UAG) codon that is read through during translation.

84 Two independent observers (O.A., P.K.) read through each article and classified the articles ac

85 interaction with the ribosome influence PTC read-through efficiency.

86 old by placing the murine leukemia virus UAG read-through element upstream of the first UGA codon or

87 1-beta isoform was derived from exon 2 and a read-through event of intron 2.

88 omosomal rearrangements, and transcriptional read through events.

89 AHL signaling, QrpR, and transcriptional read-through events integrate to ensure AHL-dependent ex

90 TC mutations in different contexts exhibited read-through expression of ATM fragments, with three of

91 chanical coupling tuned to ensure an optimal read-through frequency.

92 of acpB appears to occur via transcriptional read-through from atxA-dependent start sites 5' of capB.

93 quences in some T-DNA constructs, transcript read-through from selectable markers is also possible, w

94 al activation in response to cellobiose, but read-through from the celA promoter contributes to expre

95 quences, relieving repression and increasing read-through, hasA transcription, and capsule production

96 lates RNA transcript cleavage and polymerase read-through have been well characterized, its in vivo r

97 g events such as frameshifting or stop-codon read-through have occurred, elucidating alternative tran

98 e gas sensors show an optic response that is read through high-resolution digital color detectors.

99 ragment of DNA polymerase I, Sequenase could read through homopolymeric regions with more than five T

100 uced, normally rare, and naturally unstable "read-through" human acetylcholinesterase variant, AChE-R

101 d rate of -1 programmed ribosomal frameshift read-through in a dual-luciferase assay for observing tr

102 ifferentiation-dependent polyadenylation and read-through in HPV-31.

103 rs lead to an 11-fold increase in terminator read-through in in vitro transcription reactions.

104 nes, most of which result in transcriptional read-through in protein-coding genes.

105 G418 increased read-through in selenium-replete cells as well as in the

106 e caused by a higher frequency of stop codon read-through in these species than in yeast, possibly be

107 ng control closely correlated with increased read-through, indicating that a functional NRS is necess

108 Using the translational read-through inducing drugs (TRIDs) G418 and PTC124 (Ata

109 Translational read-through inducing drugs (TRIDs), such as PTC124, wer

110 therapeutic potential of the nonsense codon read-through-inducing drug, PTC124, in treating PXE.

111 s the ability of the viral RNA polymerase to read through intergenic junctions.

112 ERV long terminal repeat (LTR) sequences and read-through into known gene sequences.

113 tion sequences allowed significant levels of read-through into the late region in undifferentiated ce

114 ination of transcription in order to prevent read-through into the next gene, which could possibly di

115 A level, which appears to result from longer read-through into the telomere downstream of the active

116 evels, which determine whether transcription reads through into the mgtA coding region or stops withi

117 er elongation rate and was less efficient at reading through intrinsic elongation blocks.

118 However, extensive transcriptional read-through invalidated similar correlations at later t

119 e of the recoding signals known to stimulate read-through is a hexanucleotide sequence of the form CA

120 This 'read-through' is in competition with termination and is

121 ICL influences which polymerases are able to read through it.

122 To effect terminator read-through, lambdaQ must gain access to RNA polymerase

123 s fused to TMOF at the C terminus by using a read-through, leaky stop codon that facilitated expressi

124 n recurrent nonsense mutation, p.R1141X, the read-through may result in substitution of the arginine

125 transcript by a hitherto unrecognized intron read-through mechanism.

126 we have directly tested the transcriptional read-through mechanism.

127 anscriptome sequencing approach, we observed read-through mRNA chimeras, tissue-type restricted chime

128 t analysis of cp-52 detected multiple unique read-through mRNAs containing SH and G sequences, consis

129 ynthesize an extended protein as a result of read-through mutations at the stop codon.

130 ad aligner capable of handling multi-mapping reads, through new and compact index structures that red

131 To thoroughly test the ability of PTC124 to read through nonsense mutations, we conducted a detailed

132 lts are consistent with a mechanism in which read through of a pseudouridylated stop codon in bacteri

133 26S proteolytic activity leads to increased read through of a transcription termination site.

134 to EutX and, instead, causes transcriptional read through of multiple eut genes.

135 p codons, and one was predicted to result in read through of the normal translational termination cod

136 e phenotypic effects of [PSI+] may be due to read-through of "normal" stop codons, thereby producing

137 reading frame which exploits transcriptional read-through of a minimal polyadenylation signal from a

138 Here, we identify and characterize native read-through of a nonsense mutation (R201X) in the human

139 notypes, but many do not appear to be due to read-through of a single stop codon, but instead are mul

140 s that this alternative transcript arises by read-through of a splice donor site.

141 ucleosome repositioning, and transcriptional read-through of associated DNA.

142 nd identified multiple peptides derived from read-through of conventional stop codons.

143 e read-through compounds, designed to induce read-through of mRNA around premature termination codons

144 all traits tested involved [PSI(+)]-mediated read-through of nonsense codons.

145 Thus, our results suggest that read-through of nonsense mutations in ABCC6 by PTC124 ma

146 Molecules that induce ribosomal read-through of nonsense mutations in mRNA and allow pro

147 the [PSI] genetic element that enhances the read-through of nonsense mutations in the yeast Saccharo

148 The ability of this drug to facilitate read-through of nonsense mutations was examined in HEK29

149 ecific mutations or drugs developed to allow read-through of nonsense mutations, whereas other therap

150 n of polyadenylation at (pA)p, necessary for read-through of P41-generated capsid gene pre-mRNAs whic

151 In the absence of stalling, read-through of poly(A) produces a poly-lysine tag, whic

152 Aminoglycoside antibiotics facilitate read-through of premature stop codons in prokayotes and

153 e of their capacity to enhance translational read-through of premature termination codons (PTCs), the

154 of the T-box anti-terminated state allowing read-through of regulated genes.

155 tion as its depletion causes transcriptional read-through of selected gene terminators and because it

156 the mother cell location are inferred to be read-through of spoIIIG, the structural gene for sigma(G

157 acy and tolerability of a drug which induces read-through of stop codons in Duchenne muscular dystrop

158 Sup35 adopts the prion state, [PSI(+)], the read-through of stop codons increases, uncovering hidden

159 s of polyadenylation factor crosslinking and read-through of termination sequences.

160 of an amber suppressor tRNA should result in read-through of the 326 open reading frames (ORFs) that

161 is reinforced by experiments indicating that read-through of the CTS can be efficiently promoted by s

162 eved by aminoglycoside-induced translational read-through of the E375X premature stop codon, restorin

163 units, which may contribute to the increased read-through of the early sequence.

164 RNA polymerase II to the ATG start site and read-through of the ET-1 coding region.

165 sion either by ribosomal frameshifting or by read-through of the gag stop codon.

166 merged into a single ORF (termed a mORF) by read-through of the intervening stop codon, and may comp

167 Read-through of the missing codon occurred only when the

168 NP1 is required for read-through of the MVC internal polyadenylation site an

169 P1 is required for both the splicing and the read-through of the proximal polyadenylation site of the

170 tream of genes to act as a backup in case of read-through of the real stop codon.

171 n of therapeutics for SMA designed to induce read-through of the SMNDelta7 stop codon to show increas

172 IIsnR), and CPL4RNAi plants showed increased read-through of the snRNA 3'-end processing signal, lead

173 idine (Psi) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRN

174 Read-through of this transcript into the region correspo

175 We also demonstrate unexpected read-through of transcription at the Rho-independent ter

176 romising therapeutic target that would allow read-through of transcripts to enhance protein function

177 ral delivery of the missing dystrophin gene, read-through of translation stop codons, exon skipping t

178 s rrn nut-like site enhances transcriptional read-through of untranslated RNA consistent with an anti

179 eam edge of the transcription bubble lead to read-through of various types of pauses and termination

180 r translational cofactors are necessary for "read-through" of a UGA stop codon that specifies selenoc

181 A codon into a reporter construct allows for read-through only in the presence of selenium.

182 ORF9A and ORF9 mRNAs, whereas expression of read-through ORF9A/9/10 and ORF9/10 transcripts was incr

183 transcript levels measured at the 5' end or reading through oriC disappeared within one mass doublin

184 A-AAV RNAs generated from upstream promoters read through (pA)p, as seen for AAV2.

185 hat encompass the NRS also lead to increased read-through past the viral polyadenylation site, sugges

186 olymerase (RNAP), thus helping the enzyme to read through pausing and arresting sites on DNA.

187 is such that at physiological pH the active, read-through permissive conformation is populated at app

188 significant variability in their stop codon read-through phenotypes depending on the background geno

189 The level of this read-through product is proportional to CAG repeat lengt

190 These read-through products did not influence the efficacy and

191 vivo transmission a site in the coat protein-read through protein (CP-RT) of beet necrotic yellow vei

192 protein, the only known, naturally occurring read-through protein in eukaryotes, was sequenced by ion

193 The rabbit beta-globin read-through protein, the only known, naturally occurrin

194 ation codons can be ignored to obtain larger read-through proteins.

195 drug amlexanox was tested for its ability to read-through PTC mutations in cells derived from patient

196 , the structure of local hairpins within the read-through region is more variable in delta 159 RNA th

197 reading frames and extensive transcriptional read-through resulting in overlapping mRNAs.

198 thway leading to lambdaQ-mediated terminator read-through results in the formation of a highly stable

199 ates the rate at which reverse transcriptase reads through RNA secondary structure.

200 e observations also suggest that the lengthy read-through RNAs postulated to be intermediates in retr

201 ints specific for individual alleles and can read through sequences previously not accessible for ana

202 AT/Met treatment decreased selenium-mediated read-through significantly (p < 0.001) in luciferase con

203 In some cases, the transcriptional read-through significantly reduced expression of the ass

204 y more functional protein referred to as SMN read-through (SMN(RT)).

205 lots detect normal PAI transcripts and dsRNA read-through species, but not diced smRNAs, suggesting t

206 rom read-pair, read-depth and one end mapped reads through statistical likelihoods based on Poisson f

207 actor IIS provided roughly the same level of read-through stimulation for transcript elongation in th

208 Ribosomes can read through stop codons in a regulated manner, elongati

209 the 64 possible codons immediately 3' of the read-through stop codon.

210 Aminoglycosides also induced stop codon read-through, suggesting that these antibiotics alleviat

211 on region significantly reduced the level of read-through, suggesting that these factors modulate rea

212 me DNA synthesis and, in a reaction we call "read-through synthesis," forks established while the sub

213 mance by re-learning eye-movement control in reading through systematic oculomotor practice.

214 Transcripts which read through the +93 site quantitatively terminate at a

215 h polymerase failed to terminate and instead read through the gene-end sequence to generate a bicistr

216 , in contrast to wild-type virus, frequently read through the leader termination site during transcri

217 linacin-3 gene had induced a frameshift that read through the original stop codon and allowed the chi

218 most polymerases are unable to recognize and read through the presence of a single caging group on th

219 t RNase H activity is not critical for RT to read through the RNA secondary structure.

220 e that Escherichia coli RNAP can effectively read through the site-specific DNA-binding proteins in v

221 ast and largely irreversible, allowing RT to read through the stable hairpin structures.

222 ing frame once or twice in a -1 direction to read through the stop codon in the gag reading frame.

223 mplexes that terminate but not by those that read through the terminator.

224 onic mRNA and resulted instead in polymerase reading through the gene junction to produce a bicistron

225 on originating at a distal upstream site and reading through the hurR-bhuR intergenic region contribu

226 ate than wild type and was more efficient at reading through the same blocks.

227 GRO-Seq analysis showed the polymerase reading through the termination signal in the downstream

228 s are transcribed by RNA polymerase II which reads through the region containing early polyadenylatio

229 olymerase activities of RT are coupled as RT reads through the RNA secondary structure.

230 ions and compete with DNA polymerase V which reads through the tandem lesion.

231 y to cryptic epitopes revealed by stop codon read-through therapies and potentially other therapeutic

232 o be a good model for aminoglycoside-induced read-through therapy.

233 ients by using aminoglycosides to induce PTC read-through, thereby restoring levels of full-length AT

234 t is required for transcription complexes to read through this region during the first pass through n

235 and all of the 10 in-frame UGA codons being read through to produce Se-P57B.

236 The read-through transcript contributes to total w transcrip

237 ed by several assays, as was a less abundant read-through transcript encompassing pilA1, pilA2, and p

238 hus, as is the case in Escherichia coli, the read-through transcript from rpsO-pnp is cleaved by RNas

239 Read-through transcript levels also significantly increa

240 RNA substrate representing a portion of the read-through transcript normally produced in S. coelicol

241 -untranslated leader segment of a trp operon read-through transcript, it can disrupt a large secondar

242 be the site for RNase IIIS processing of the read-through transcript.

243 titermination increases the synthesis of the read-through transcript.

244 cistronic gene clusters in L. major leads to read through transcription and increased expression of d

245 n defects affect gene expression and whether read through transcription is detrimental to cell growth

246 ied vector splice sites, we reduced residual read-through transcription and demonstrated an effective

247 e CID from Rtt103 (Nrd1(CID(Rtt103))) causes read-through transcription at many genes, but can also t

248 that increases the elongation rate increases read-through transcription at Sen1-mediated terminators.

249 s and, by binding to the same site, prevents read-through transcription from the distal, lmo1569 prom

250 in sea and sak transcription was a result of read-through transcription from upstream latent phage pr

251 Here we show that read-through transcription from yeast small nucleolar RN

252 at inefficiently terminates HasS, permitting read-through transcription of hasABC, and a putative pro

253 proportion of crtJ expression is promoted by read-through transcription of orf192 (aerR) transcripts

254 0463 could be increased TcdR translation and read-through transcription of the tcdA and tcdB genes.

255 or can activate the transcription or lead to read-through transcription of their downstream genes.

256 rm at any DNA sequence but are suppressed by read-through transcription or that they can overlap the

257 We observed a surprising abundance of read-through transcription originating outside and insid

258 hese transcripts and that spreading requires read-through transcription, as well as slicing by Argona

259 tergenic region resulted in higher levels of read-through transcription.

260 lation removes this attenuation and leads to read-through transcription.

261 mRNA, caused,most likely, by light-mediated read-through transcription.

262 minators are excluded from functional TUs by read-through transcriptional interference, while antisen

263 e precise boundaries of many mRNAs including read-through transcripts and location of mRNA start site

264 In contrast, few M-F read-through transcripts are detected in SV-infected cel

265 nction is ineffective; a large number of M-F read-through transcripts are produced.

266 s of polyadenylated histone mRNA and nascent read-through transcripts at the histone locus.

267 We show that Hox gene read-through transcripts can be spliced to produce inter

268 A stability, mRNA processing, and removal of read-through transcripts in S. pyogenes.

269 g attenuation regions yielded terminated and read-through transcripts of the expected lengths.

270 Rider full-length and read-through transcripts past the typical transcription

271 Abundant read-through transcripts were observed in the presence o

272 rids (R-loops), including from antisense and read-through transcripts, in a nusG missense mutant defe

273 t stages of meiosis, including antisense and read-through transcripts.

274 3-kb RNA spanning ORF10 only and three other read-through transcripts.

275 as well as in the rapid degradation of rnpB read-through transcripts.

276 with rSVhMFjCG produced an abundance of M-F read-through transcripts; this result indicated that the

277 An alternative outcome, read-through translation (or nonsense suppression), is w

278 ells from patients with RDEB that respond to read-through treatment.

279 a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference-mediated gene

280 Read-through type VII collagen was readily detectable in

281 [PSI (+)] prion, empowers yeast ribosomes to read-through UGA stop codons.

282 nfluence on the ability of the polymerase to read through uracil and hypoxanthine, the same kinetic p

283 they resulted from extensive transcriptional read-through, use of downstream polyadenylation sites, a

284 The usually rare read-through variant of acetylcholinesterase (AChE-R) is

285 ons up to 20 mug ml(-1), and the facilitated read-through varied not only with dose but also with seq

286 Moreover, we identified a read-through w transcript initiated from a retrotranspos

287 Upon differentiation, this read-through was increased by approximately 50%, indicat

288 eosome in the absence of tails, but complete read-through was not substantially increased by tail rem

289 The full-length eRF1 generated by UAA read-through was present at sub-wild-type levels.

290 antly, the suppression of selenium-dependent read-through was similar whether an SV40 promoter or the

291 adjacent active chromatin or transcriptional read-through, which may be free of selective biases.