ライフサイエンスコーパス: reagent

1 1 as the catalyst and HBpin as the terminal reagent.

2 ndicating its potential use as a therapeutic reagent.

3 yldisiloxane serves as a convenient reducing reagent.

4 CPGCC) tetracysteine (TC) tag and FlAsH-EDT2 reagent.

5 es such as Bronsted activation of the iodane reagent.

6 en extra-lattice Al-OH species and an alkane reagent.

7 functionalization with arene as the limiting reagent.

8 in water with bicarbonate as the only added reagent.

9 the pnictogen-chalcogen bond in the transfer reagent.

10 with them without addition of any catalytic reagent.

11 microwave irradiation and excess Lawesson's reagent.

12 were also explored through use of scavenger reagents.

13 onventional use of halides and toxic cyanide reagents.

14 and mitigates the need for refrigeration of reagents.

15 uranyl salts, silyl halides, and alkylating reagents.

16 rdiagnosed because of the lack of diagnostic reagents.

17 ositive cases without stable isotope-labeled reagents.

18 ians, and facilities to store heat-sensitive reagents.

19 gen, and thereby generating Fenton chemistry reagents.

20 enhancements compared to previously reported reagents.

21 and development of improved versions of the reagents.

22 agent, a copper catalyst, and alkyl Grignard reagents.

23 f the domain and the difficulty of producing reagents.

24 facilitating reactions with non-nucleophilic reagents.

25 potentially be performed without sacrificial reagents.

26 de and requiring only tens of microliters of reagents.

27 t without the need for addition of exogenous reagents.

28 ions in a short time using minimal amount of reagents.

29 are becoming a salient option as immunoassay reagents.

30 e unsaturated organoboron compounds serve as reagents.

31 among the weakest known X-H bonds of stable reagents.

32 e reactivity of the iodine-based hypervalent reagents.

33 future development as H2 S-based therapeutic reagents.

34 ficial molecular systems without sacrificial reagents.

35 aningful parameters and/or rely on expensive reagents.

36 the absence of suitable heteroatom-transfer reagents.

37 e scope for both oxyamines and sulfenylation reagents.

38 and require expensive equipment or hazardous reagents.

39 oxic and often difficult to scale pyrophoric reagents.

40 cides, with low consumption of biodegradable reagents.

41 nd using prereduction and signal-enhancement reagents.

42 by SPE combined with 500muL of derivatizing reagent (0.2M NAC and 0.2M NaHCO3 in water) and reaction

43 zed directly from two inexpensive commercial reagents: 1-butyl-3-methylimidazolium bromide and sodium

44 high-purity, relatively inexpensive isotopic reagents ((18)O water and deuterated formaldehyde) and r

45 iolation of 1,3-dienes with a sulfur diimide reagent, a copper catalyst, and alkyl Grignard reagents.

46 a synergic system based on two bifunctional reagents, a thiosemicarbazide-functionalized nopoldiol a

47 t the concept of loop control via continuous reagent addition could find broad application in the syn

48 (2) Utilization of WBQ in postcolumn reagent addition methods where the reagent produces a si

49 actions with the fluorophore-conjugated DpTz reagents afforded the fluorescently labeled GCGR and GLP

50 chlorides with gem-difluorinated organozinc reagents affording difluorinated ketones is described.

51 nt good manufacturing practices-manufactured reagents after assay standardization and reproducibility

52 ing the upper determination limit; a limited reagent amount truncates peaks from high analyte concent

53 is procedure requires no additional catalyst/reagent and can be applied to substrates with a wide ran

54 s are developed using NBS as the brominating reagent and catalytic amount of Cu(II) /phenanthroline c

55 lowed by reaction with an organocopper (RCu) reagent and final reduction with triacetoxyborohydride.

56 c T-ALL patient cells without a transfection reagent and induce Plk1 knockdown on both protein and mR

57 rs and saved their laboratory over $8,000 in reagent and labor costs during an 11-month postintervent

58 turized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kine

59 alkyne-containing neurosteroid photolabeling reagent and used to identify peptide-steroid adducts in

60 Using a novel sp(2)-sp(3) hybridized diboron reagent and water as a proton source, a broad range of a

61 es (n = 158) were tested with research-grade reagents and 122 independent validation set samples were

62 azo compounds using ethynylbenziodoxol-(on)e reagents and a simple copper bisoxazoline catalyst.

63 nes as materials by adsorbing substrates and reagents and activating them by charge transfer is also

64 3)) cross-coupling reaction between Grignard reagents and alpha-chloroesters.

65 Finally, by using new reagents and assays, we show that the two MMPs cleave di

66 ake use of practical, commercially available reagents and can engage a variety of substituted cyclopr

67 and highlight the future potential of these reagents and catalysts in organic synthesis.

68 dditives, nitrogen-protecting groups, excess reagents and harsh workup conditions.

69 idic chip, prefilled with biomarker-specific reagents and magnetic beads, can be processed fully auto

70 tibodies remain the most widely used of such reagents and many show excellent performance, although s

71 complicated assays and the transportation of reagents and mitigates the need for refrigeration of rea

72 tion steps that are required to remove these reagents and release DNA in typical NAAT sample preparat

73 hey provide advantages as low consumption of reagents and samples, high speed of analysis, possibilit

74 which may both influence binding of affinity reagents and serve to validate the target specificity of

75 how that tableting stabilizes heat-sensitive reagents and simplifies a broad range of assays, includi

76 gration (isomerization) in both the 1-alkene reagents and the internal-alkene products.

77 trophilic than other known alkyne-activating reagents and then providing chloride for post-rate-deter

78 ogically active compounds, used as spin-trap reagents and therapeutic agents.

79 nd the low requirements in terms of samples, reagents and time.

80 titative yields (with Cu(II) as the limiting reagent) and selectivity combined with turnover numbers

81 ifluoromethyl)phenylacetyl chlorides (Mosher reagents), and DP4 + NMR calculations.

82 fluorination for a representative aryl boron reagent, and on the labeling of more than 50 (hetero)ary

83 ving the mixing of proteins, crystallization reagents, and buffer in nanoliter volumes and the subseq

84 feature operational simplicity, inexpensive reagents, and enhanced scope as evidenced by examples in

85 al activity, binding to p40 and p19 specific reagents, and maintains biophysical properties similar t

86 ily prepared on large scale from inexpensive reagents, and reacts in the title reaction with acetonit

87 uPAD effectively reduces the cost, volume of reagents, and time required to run ELISA for food sample

88 tandard, solubilization approach, iodometric reagents, and wavelength detection affected total starch

89 osphine ligand, the order of addition of the reagents, and, most importantly, the nature of the palla

90 reat HIV-1 infection, standardized reference reagents are a cost-effective way to maintain robustness

91 ", small quantities of both template DNA and reagents are encapsulated within a droplet or microwell,

92 To examine whether PD-L1 antibody reagents are interchangeable by quantitatively comparing

93 Molecular recognition reagents are key tools for understanding biological proc

94 e reactor's inlet, and paraffin-encapsulated reagents are prestored within the reactor.

95 nstruction of a tandem library, in which two reagents are randomly-displayed on the phage surface.

96 unctive cross-couplings that employ Grignard reagents are shown to furnish an array of nonracemic chi

97 All of the reagents are stable during long-term storage and can be

98 Specifically, bifunctional amino reagents are used for sequential nucleophilic and electr

99 Protein affinity reagents are widely used in basic research, diagnostics

100 The role of hypervalent iodine reagents as oxidants has been widely recognized for more

101 s of the protocol stems from the use of a Pd-reagent based on a dihaloarene, which enables direct "nu

102 Aptamers are a promising class of affinity reagents because they are chemically synthesized, thus m

103 that ZapD acts as a molecular cross-linking reagent between FtsZ protofilaments to enhance FtsZ asse

104 paring with heteroatom sources from chemical reagents, biomass is notably inexpensive and abundant, c

105 In the presence of trifluoromethylsulfonium reagents, boronate complexes derived from 2-lithio furan

106 thenoyltrifluoroacetone (TTA) as a chelating reagent by ETAAS.

107 y was confounded by the reduction of the MTT reagent by honey's reducing sugars and phenolic compound

108 and to catalyze the application of enoldiazo reagents by the wider synthetic community.

109 The reagent can be easily prepared on large scale from inexp

110 ochemical states and find that the prochiral reagent can sample several complexation geometries durin

111 ith our technique, premeasured quantities of reagents can be encapsulated in compressed tablets.

112 Furthermore, SHERLOCK reaction reagents can be lyophilized for cold-chain independence

113 To ensure that the sealing of the reagent chamber is maintained, aqueous reagents should b

114 rred within five reaction chambers and three reagent chambers via the chip's automatic dispensation a

115 described a readily synthesized and handled reagent class which can accomplish the controlled and se

116 Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter dro

117 The Bradford reagent, comprised of the Coomassie Brilliant Blue G-250

118 They miniaturize assays to reduce sample and reagent consumption and increase throughput, and additio

119 ision (RSD<5%) and accuracy (RE<5%), reduced reagent consumption and reaction times (<6min), and exce

120 as the potential to significantly reduce the reagent consumption and shorten the reaction and analysi

121 and miniaturization with minimal sample and reagent consumption by inexpensive instrumentation.

122 ular analysis in shorter time and with lower reagent consumption than a standard laboratory.

123 ctive compounds can greatly benefit from low reagents consumption and high throughput capacity of dro

124 affinity selected to isolate pairs that both reagents contribute to binding the target.

125 mistry of the resulting products is entirely reagent-controlled, this process may be viewed as a gene

126 transduction, requires minimal time, has low reagent costs and improves cell recovery (up to 60%), as

127 e multimaterial device containing the Griess reagent could be stored for over 4 days but increased th

128 These advances with versatile, preassembled reagents could greatly reduce the time and cost of in vi

129 for paper cutting, followed by colorimetric reagent deposition using technical pens.

130 ylic coupling reaction with an organolithium reagent derived from the alkyl iodides (R)- or (S)-30, w

131 Regioselective carbyne-transfer reagents derived from (3,3,3-trifluoroprop-1-yn-1-yl)ben

132 tion of the proton and electron to different reagents does not significantly inhibit the proton-coupl

133 ure eliminates (i) the use of PCR-inhibiting reagents (e.g., chaotropic salts and alcohol) and (ii) t

134 ng time of aluminum-DEMAX complex, amount of reagent, effect of temperature etc.

135 diment Separator and treatment with Fenton's reagent enabled analysis via Attenuated Total Reflection

136 re easily functionalized using electrophilic reagents, enabling divergent access to varied substituti

137 Molecular dynamics simulations with reagent excitation by way of selected vibrational normal

138 extraction conditions (20 buffers, defatting reagents, extraction time/temperatures, processing, extr

139 O-Ethylation of phthalides with Meerwein's reagent followed by reaction of the ensuing salts with p

140 orts tert-butyl nitrite (TBN) as a multitask reagent for (1) the controlled synthesis of N-nitrosoami

141 thium phosphinidene as a phosphorus transfer reagent for a wide range of organic and inorganic substr

142 Moreover, it is a convenient reagent for analysis of the total amine content by quant

143 The viability of the reagent for biological in vivo imaging application was a

144 oying phenylboronic acid as a phase-transfer reagent for Fischer glycosidations in low-polarity organ

145 ed that EuDO3A could be used as an NMR shift reagent for imaging extracellular lactate produced by ca

146 This makes it a useful reagent for measuring both potency and stability.

147 gent make it an ideal candidate for use as a reagent for super-resolution structured illumination mic

148 is reported the design and application of a reagent for the direct functionalization of pyridines.

149 3SiCl, was also effectively used as the X-OH reagent for the generation of 1 and HN(SiMe3)2.

150 Designed Bfl-1 binders provide reagents for cellular profiling and leads for developing

151 on of fluorinated sulfur ylides as versatile reagents for Corey-Chaykovsky cyclopropanation reactions

152 sters and amides have proven to be versatile reagents for cycloaddition reactions that allow highly e

153 olerant and stable to reactive acyl chloride reagents for extended periods.

154 ree pendant side-arms were examined as shift reagents for lactate detection.

155 lternative binding proteins as complementary reagents for many applications.

156 Current chronic use of immunosuppressive reagents for preventing islet allograft rejection is ass

157 need of specialised equipment, and expensive reagents for serological and molecular diagnosis respect

158 rominence alongside complementary biological reagents for target validation in drug discovery.

159 proper use and analysis of loss-of-function reagents for the determination of gene function.

160 enoid scaffolds as efficient multifunctional reagents for the direct primary amination and hydroxylat

161 solution-based assembly requires heating the reagents for weeks and gives lower yields.

162 ompounds, when activated with alkyl aluminum reagents, form highly active catalysts for the polymeriz

163 The proposed device allows an entirely reagent-free analysis.

164 A label-free and reagent-free peptide mimotope capacitive biosensor has b

165 activated bioconjugation reaction as a mild, reagent-free strategy to attach oligonucleotides to gold

166 d up to 10-plex using tandem mass tags (TMT) reagents, further increasing throughput, albeit with som

167 The use of an excess of the reducing reagent gave a very functionalized allylic alcohol deriv

168 oxyamination reaction with the same nitrene reagent generated in stoichiometric amounts.

169 Donor-substituted diazo reagents, generated in situ from sulfonyl hydrazones in

170 While not surprising, this aspect of these reagents has not been fully considered and exploited in

171 ent, [hydroxy(tosyloxy)iodo]benzene (Koser's reagent), has been developed.

172 cids in the presence of the peptide coupling reagent HATU and NHC generated from the chiral triazoliu

173 The reagents have been successfully validated in tomato (Sol

174 The results show that the new reagents have good reactivity and generate products with

175 were carried out with commercially available reagents, highlighting the accessibility of the BONCAT m

176 ldoximes using organohypervalent iodine(III) reagent, [hydroxy(tosyloxy)iodo]benzene (Koser's reagent

177 opyridines takes place in the absence of any reagent in a cascade SN2'-6-endo-trig fashion and is com

178 dominant stereocontrol of the lithium amide reagent in both cases, thus augmenting the accessible ra

179 e utility and practicality of nanobody-based reagents in bioanalytical chemistry is demonstrated.

180 nucleic acids (XNA) hold tremendous value as reagents in future biotechnology applications.

181 epurposed the approximately 650 boronic acid reagents in our collection as a directed fragment librar

182 he reactivity and stereoselectivity of these reagents in oxidative rearrangements of alkenes to alpha

183 e diagnostic signals and release therapeutic reagents in response to the cancer-associated stimuli.

184 ide surfaces are treated with reactive metal reagents in the gas phase by ALD to form an outer metal

185 lective conjugate additions of lithium amide reagents in the key stereodefining steps.

186 that permit the direct use of organolithium reagents in the palladium-catalyzed cross-coupling react

187 re, which combines the assembly of exogenous reagents in the primary (phenolates) and secondary (Lewi

188 branes on its own, addition of the palladium reagent induces an efficient cell internalization of thi

189 FeS2 dissolution can act as a natural Fenton reagent, influencing the oxidation of third-party specie

190 process can be repeated by introducing fresh reagent into the microchamber.

191 bility of selectively delivering therapeutic reagents into cancer cells or tumor tissues while simult

192 roplet assays, microfluidic devices must add reagents into droplets, remove fluid from droplets, and

193 generated by direct injection of CRISPR/Cas9 reagents into mouse zygotes.

194 species (CI)H(+) using protonated amines as reagent ions.

195 alkynes using the fluoroform-derived [CuCF3] reagent is achieved.

196 mmetric propargylation using allene gas as a reagent is reported.

197 es and how the development of novel Ub-based reagents is greatly assisting in uncovering unknown aspe

198 ction conditions using the readily available reagents is reported.

199 jugated alkenes, aryl iodides, and alkylzinc reagents is reported.

200 methodology, which also uses low-cost, "wet" reagents, is scalable and done at ambient pressure.

201 l thiosemicarbazide-functionalized nopoldiol reagent labeled with fluorescein dye.

202 inducing hydroxyapatite formation at higher reagent levels (Ca(2+) = 200 mM), as also seen with pept

203 of air- and moisture-sensitive catalysts and reagents, limiting both adoption by the non-expert as we

204 rrors in the units of concentration of three reagents listed in the Methods.

205 n recent years become a versatile and useful reagent, mainly for reducing carbonyl-type substrates.

206 operties of the BODIPY-based chemodosimetric reagent make it an ideal candidate for use as a reagent

207 trategies with lower polarity organometallic reagents (Mg(CH2 SiMe3 )2 and Al(TMP)iBu2 ), key interme

208 cifically, three different covalent labeling reagents, namely diethylpyrocarbonate, 2,3-butanedione,

209 able analytical cartridge containing all the reagents necessary for the complete analysis.

210 hone paper-based biosensor, in which all the reagents necessary to complete the analysis are co-entra

211 he extreme simplicity of assay execution (no reagents need to be added) and flexibility of fabricatio

212 amplification functions was loaded with all reagents needed for sample processing and multiplex nest

213 xpensive, commercially available, analytical reagent Nitron undergoes a previously unrecognized trans

214 ometric methods such as the use of expensive reagent not environmentally friendly, undefined reaction

215 s, unlike additions of other organomagnesium reagents, occur at rates approaching the diffusion rate

216 ng, we perform cross-linking by bifunctional reagents of multiple predicted pairs of cysteines in TMH

217 steric substitution of the alkynyl Grignard reagent on catalytic performance were investigated.

218 The effects of exogenous reagents on product distributions are consistent with th

219 NAP-tag approach to install the boronic acid reagent onto the extracellular domain of the Beta-2 adre

220 (-1) without the use of sacrificial chemical reagents or carbon feedstocks other than CO2 This approa

221 sensitivity and without the use of exogenous reagents or enzyme amplification steps.

222 limits for their targets without the use of reagents or wash steps.

223 ethylpyrocarbonate, 2,3-butanedione, and the reagent pair EDC/GEE, are used together to pinpoint the

224 Standard reference reagents, particularly virus panels to study neutralizat

225 pplied voltage, allosteric effects, chemical reagents, pH, and mechanical force, it is still challeng

226 With the unsymmetrical diboron reagent PinB-BDan (Dan = naphthalene-1,8-diaminato), ste

227 esium exchange with i-PrMgCl, or aryllithium reagents prepared in situ by bromine/lithium exchange wi

228 Commercial Grignard solutions, Grignard reagents prepared in situ by halogen/magnesium exchange

229 ostcolumn reagent addition methods where the reagent produces a significant detector background.

230 tional Genomics Resources website serve as a reagent production tracking system, screen data reposito

231 or precious metal catalysts, ligands, excess reagents, protecting and/or directing groups.

232 oratory infrastructure, equipment, supplies, reagents, quality assurance, staffing and training, ofte

233 dopt a two-electron donor character once the reagent R3MH (M = Si, Ge, Sn) enters the ligand sphere.

234 sary to streamline unit operations and store reagents refrigeration-free.

235 NAC, an antioxidant reagent, rescued HDG and PLK2 overexpression-induced kid

236 on state analogs and Horner-Wadsworth-Emmons reagents, respectively.

237 ns employ [B(pin)]2-methane as a conjunctive reagent, resulting in the formation of two C-C bonds at

238 d a novel class of engineered antibody-based reagents ('Seldegs') that induce the selective degradati

239 vated pyridines, Eschenmoser's salt, Togni's reagent, Selectfluor, diisopropyl azodicarboxylate (DIAD

240 ults support a mechanism in which the ClBcat reagent serves a bifunctional role by sequentially activ

241 a molecular map of 100 spots, excluding the reagent setup.

242 f the reagent chamber is maintained, aqueous reagents should be avoided.

243 e is challenging with non-viral gene-editing reagents, since most of the edited sequences contain var

244 ns of radicals generated using the classical reagent, SmI2.

245 tor CD4(+) T cells, an MHC class II tetramer reagent specific for T. gondii did not recognize Tregs i

246 ructural analysis of proteins using chemical reagents specific for lysine or cysteine residues, ident

247 vortexing of the magnetic beads and on-chip reagent storage and actuation were developed.

248 te silicon intermediate consisting of silane reagent, substrate, and the tert-butoxide catalyst.

249 hiocyanate complex was done by novel organic reagents such as N-phenylacetamide, N-alkylacetamide, (a

250 vatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone).

251 Cl3 with AgSbF6 was identified as a suitable reagent system to promote the present reaction.

252 ), an inexpensive and commercially available reagent, tetrabromophthalic anhydride (TBPA) was used fo

253 Here, we report the development of a new reagent that in combination with mass spectrometry (MS)

254 s convenient route employs readily available reagents that afford polylactide in good yields with nar

255 t only of new enzyme activities, but also of reagents that are both sufficiently reactive for the des

256 ing a sandwich assay is identifying pairs of reagents that bind non-competitively to the target.

257 he 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte

258 copper-catalyzed coupling of arylbismuth(V) reagents that is compatible with the hemilabile OPh bloc

259 (absence of caustic, viscous, or additional reagents), the simplicity (does not require any chemical

260 When using the nonaqueous reagent, the multimaterial device containing the Griess

261 uses both aryl groups of the diaryliodonium reagent to generate triarylamines starting from simple a

262 vesicles results in specific binding of this reagent to some of the pendent cis-diol groups on the hy

263 Addition of two equivalents of an alkylating reagent to UO2 X2 (L)2 (X=Cl, Br, I, OTf; L=triphenylpho

264 izing the TC-labeling FlAsH-EDT2 biarsenical reagent to visualize VFs, evidence is provided of dynami

265 Highly chemoselective addition of Schwartz's reagent to widely available azlactones is described.

266 scaffolds, represent complementary affinity reagents to antibodies for various molecular and cell bi

267 style experiments with deuterated gases and reagents to compare the spark-discharge products of a "d

268 be used in place of trifluoromethylsulfonium reagents to effect similar three-component coupling reac

269 of DBU, is dehydrated by activated sulfonium reagents to generate the corresponding isocyanate.

270 shelf-stable and ready-to-use (shelf-ready) reagents to greatly simplify the bioanalysis procedures,

271 d Openspritzer to deliver precise amounts of reagents to hippocampal neurons to elicit time- and dose

272 Nickel-catalyzed addition of arylboron reagents to ketones results in aryl olefins directly.

273 ion of LiCF2P(O)(OEt)2 and BrMgCF2P(O)(OEt)2 reagents to N-t-butanesulfinyl glycosylamines is describ

274 primers', which code for previously-selected reagents, to prime second strand synthesis of a single-s

275 ine borane with gamma-iodo-alpha-amino ester reagents under phase-transfer catalysis (PTC).

276 in a massively parallel manner with minimal reagent use.

277 al standard (IS) with o-phthalaldehyde (OPA) reagent using high-performance liquid chromatography-flu

278 nsmetalation with the corresponding Grignard reagents using ZnCl2 forms organozinc compounds which ar

279 iaturized format allows for small sample and reagent volumes.

280 successive reduction of different diazonium reagents were also observable and will be valuable for a

281 The reagents were selective for the heavy and light chain of

282 Both reagents were shown to be chemically stable and nontoxic

283 All reagents were stored within the device.

284 These reagents were used to examine whether overexpression of

285 n of certain drug-linker elements within the reagent, were first evaluated in vitro using anti-prolif

286 oxidants, catechol/Cu(2+)/NADPH and Fenton's reagent, were located and compared.

287 eaction utilizes a masked acyl cyanide (MAC) reagent, which enables the one-pot preparation of alpha-

288 n oligo(diyne) with a low-valent zirconocene reagent, which gives a PAH with one or more annulated zi

289 by the specific substituents of the starting reagents, which has been rationalized analyzing the ener

290 hat aerobically generated hypervalent iodine reagents will expand the scope of aerobic oxidation chem

291 er-mediated (18)F-fluorination of aryl boron reagents with (18)F-fluoride as a model reaction.

292 lization of 4-fluoroaryl Grignard or lithium reagents with 2,2-disubstituted malononitriles is descri

293 r the cross-coupling of tertiary organoboron reagents with aryl halides is reported.

294 purification; refrigeration-free storage of reagents with just-in-time release; and enzymatic amplif

295 her slow off-rate modified aptamer (SOMAmer) reagents with subnanomolar affinity for Mycobacterium tu

296 the diastereoselective reaction of arylboron reagents with substrates containing a 1,3-enyne tethered

297 al tagging with a reduced excess of labeling reagent without intramolecular side reactions.

298 WBQ can minimize added reagent without sacrificing the upper determination limi

299 aused by inefficient microscopic mixing of a reagent X with two competing substrates A and B, may bec

300 e and have demonstrated that different ddPCR reagents yield droplets of somewhat different size.