ライフサイエンスコーパス: real-time PCR

1 ghly abundant with TAR RNA as detected by RT-real time PCR.

2 nd islet Reg gene expression was measured by real time PCR.

3 ed and miRNA profiling was done using taqman real time PCR.

4 ave results comparable with each single-plex Real Time PCR.

5 es was measured using multiplex quantitative real time PCR.

6 NanoString technology and validated by using real-time PCR.

7 enotyped for the FTO rs9939609 variant using real-time PCR.

8 ELISA, immunoblot, immunohistochemistry and real-time PCR.

9 , enzymatic assays (ELISA), and quantitative real-time PCR.

10 ected and validated by targeted quantitative real-time PCR.

11 Gene expression levels were evaluated by real-time PCR.

12 and sequenced and fungal load determined by real-time PCR.

13 tion with a peptide nucleic acid probe-based real-time PCR.

14 Expression of IDO gene was measured by real-time PCR.

15 n of the histamine receptors was measured by real-time PCR.

16 ered for the detection of food allergens via real-time PCR.

17 108; 70 patients) compared to the reference real-time PCR.

18 zed by Western blotting, flow cytometry, and real-time PCR.

19 A (ADAR)(rs1127309TC) genes were analyzed by real-time PCR.

20 atients were screened for colonization using real-time PCR.

21 7-expressing cells, was further confirmed by real-time PCR.

22 d to those of microbiological culture and/or real-time PCR.

23 gene expression of key anabolic proteins by real-time PCR.

24 merase chain reaction (PCR) and quantitative real-time PCR.

25 of genes encoding catalases were examined by real-time PCR.

26 late counts, flow cytometry and quantitative real-time PCR.

27 ation and reverse transcriptase quantitative real-time PCR.

28 RNA (mRNA), was evaluated using quantitative real-time PCR.

29 ination in the model foods was determined by real-time PCR.

30 nom MassArray and pfmdr1 gene copy number by real-time PCR.

31 by HDM was quantified by using quantitative real-time PCR.

32 by means of extracellular flux analysis and real-time PCR.

33 sion profiles were evaluated by quantitative real-time PCR.

34 AKR1C3 expression was measured by real-time PCR.

35 ity for amplification by qualitative PCR and real-time PCR.

36 CC tissues compared to normal oral mucosa by real-time PCR.

37 persed nuclear element-1 (LINE-1) quantitive real-time PCR.

38 both in situ hybridization and quantitative real-time PCR.

39 receptor 2 (VEGF-R2) in VECs was assessed by real-time PCR.

40 or the detection of Plasmodium species using real-time PCR.

41 rent median strain lifespans by quantitative real-time PCR.

42 anscripts were determined using quantitative real-time PCR.

43 e expression H4R and RANKL was determined by real-time PCR.

44 he retinae of these mice was demonstrated by real-time PCR.

45 sured by using a microarray and quantitative real-time PCR.

46 Of 120 samples tested by individual real-time PCR, 35 were positive for eight different targ

47 the three susceptible loci were measured by real-time PCR after the stimulation by M. leprae antigen

48 strains, by flow cytometry and quantitative real-time PCR allowed tentative links to the burgeoning

49 Paired swabs were tested by GAS real-time PCR, allowing semiquantitative comparisons bet

50 TaqMan real-time PCR amplification was used to detect the prese

51 rthermore, using whole-genome microarray and real-time PCR analyses of abhd11 and wild-type plants, w

52 ical Tas2r-dependent reporter expression and real-time PCR analyses reveal that human and mouse thyro

53 Real-time PCR analyses revealed a significant reduction

54 Real-time PCR analyses revealed that endogenous levels o

55 RNA sequencing and quantitative real-time PCR analyses revealed that, although the expre

56 Real-time PCR analyses showed that IFN-gamma suppressed

57 Our real-time PCR analysis indicated that, in contrast to ou

58 This was corroborated with real-time PCR analysis of human prostate tumor tissue ar

59 Quantitative real-time PCR analysis of macrophages isolated by laser

60 We performed real-time PCR analysis of miR-31-3p expression in human

61 evels of microRNAs and mRNAs by quantitative real-time PCR analysis of RNA extracted from plasma, liv

62 Quantitative real-time PCR analysis revealed an intermediate expressi

63 Consistent with this, real-time PCR analysis revealed fewer transcription/repl

64 Using western blot and quantitative real-time PCR analysis, our results indicated that knock

65 ing clinical relevance to these findings, by real-time PCR analysis, there was a strong correlation b

66 Furthermore, the LC-MS/MS and quantitative real-time-PCR analysis followed by inhibitor and antibod

67 Quantitative real-time-PCR analysis indicated that CRHOEdev unexposed

68 r responses were measured using quantitative real time PCR and multiplex assay.

69 Real time-PCR and western blot have been used to assess

70 Real-time PCR and 5' RACE and 3' RACE experiments reveal

71 PV-18 loads were measured with a LightCycler real-time PCR and classified as high (>250 copies/scrape

72 TNF-alpha, and staurosporine), quantitative real-time PCR and clustering analysis, we studied gene-g

73 ced microglial ISG responses by quantitative real-time PCR and demonstrated that both were dependent

74 Real-time PCR and ELISA analyses showed that ER stress i

75 Testing for Ebola virus was done by real-time PCR and for malaria by a rapid diagnostic test

76 ers calretinin and calbindin, as assessed by real-time PCR and immunofluorescence.

77 Quantitative real-time PCR and immunohistochemistry of tissues harves

78 ifferent human tissues by using quantitative real-time PCR and immunohistochemistry.

79 ssion of relevant chemokines by quantitative real-time PCR and immunohistochemistry.

80 regulation of IL-6 compared with control via real-time PCR and immunohistochemistry.

81 alyzed at 24 h or 21 d by using quantitative real-time PCR and immunohistochemistry.

82 red cells by immunoblotting and quantitative real-time PCR and in mouse kidneys by immunogold electro

83 With real-time PCR and in situ hybridization analysis, SlPIP2

84 med the differentially expressed genes using real-time PCR and in situ hybridization.

85 Quantitative real-time PCR and microarray analyses confirmed PEDF dow

86 Gene expression was measured using real-time PCR and renal injury assessed with histologica

87 modulatory properties was evaluated based on real-time PCR and T-cell proliferation.

88 IL-8 and IL-10 mRNA levels were assessed by real-time PCR and Toll like receptor 4 (TLR-4) protein e

89 sessed using luciferase assays, quantitative real-time PCR and western blots in vitro and in vivo.

90 Microarray analysis, supplemented by real-time PCR and Western blotting, revealed that the ex

91 Quantitative real-time PCR and whole transcriptome sequencing were us

92 Genetic markers were determined by real-time PCR and, with clinical risk factors and Asperg

93 followed by gene expression (microarrays and real-time PCR) and immunostaining studies.

94 tion sequencing, 16 S rRNA gene quantitative real-time PCR, and aerobic culturing were applied to ass

95 Gene array, real-time PCR, and ELISA analyses showed that treatment

96 cell-based regulation using flow cytometry, real-time PCR, and immune-blotting.

97 es coupled with mRNA isolation, quantitative real-time PCR, and standard PCR analyses confirmed the p

98 atory indices by using ELISA, histology, and real-time PCR; and type 2 innate lymphoid cells (ILC2s)

99 A normalised real-time PCR approach was also proposed in the range of

100 Additionally, a quantitative real-time PCR approach was proposed, allowing detecting

101 A dual-probe real time PCR assay, based on the simultaneous detection

102 iagnostics) to our routine influenza A and B real-time PCR assay (Simplexa Flu A/B & RSV Direct; Focu

103 The real-time PCR assay also identified two samples with mix

104 e Mycobacterium abscessus group, a multiplex real-time PCR assay for clarithromycin resistance showed

105 Here we report the development of a robust real-time PCR assay for determining GBS serotypes.

106 the BDG assay was more appropriate than the real-time PCR assay for monitoring the response to treat

107 Here, we describe a SYBR green-based real-time PCR assay for Plasmodium species identificatio

108 linical performance of a simple, inexpensive real-time PCR assay for the detection of 13 carcinogenic

109 t the development and validation of a TaqMan real-time PCR assay for the detection of E. rostratum in

110 he aim of the present study was to develop a real-time PCR assay for the identification and quantific

111 The paper presents a TaqMan real-time PCR assay for the quantitative determination o

112 Our results also demonstrate that the real-time PCR assay is extremely susceptible to contamin

113 panel showed an agreement of 97.4% with the real-time PCR assay regarding 464 pathogens found in the

114 The method consisted of a real-time PCR assay targeting the gene encoding for the

115 We evaluated a real-time PCR assay to predict ciprofloxacin susceptibil

116 In addition, the real-time PCR assay was applied to the analysis of comme

117 A real-time PCR assay was developed for detection of crab,

118 al culture and the previous PCR assays, this real-time PCR assay was more sensitive.

119 The LOD and LOQ of the real-time PCR assay were 0.1% and 0.4%, respectively.

120 We have designed a novel triplex real-time PCR assay which simultaneously amplifies the M

121 sequence information of ITS2, we developed a Real-Time PCR assay which successfully identified herbal

122 ped and validated the first direct multiplex real-time PCR assay with melt curve analysis for the ide

123 est, v2.0), a dual-target total nucleic acid real-time PCR assay.

124 ing microRNAs (miRNA), termed S-Poly(T) Plus real-time PCR assay.

125 PCR assay, and 65 (47%) were positive by the real-time PCR assay.

126 t5 target genes were assayed by quantitative real-time PCR assay.

127 /=2 HPV31-positive visits were measured by a real-time PCR assay.

128 g was performed using a laboratory-developed real-time PCR assay; for the postimplementation group, C

129 LISA Kit-total) and three in-house developed real-time PCR assays (singleplex assay for white mustard

130 was detected with both ELISAs and the three real-time PCR assays although mustard was not indicated

131 cal fungal isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melt

132 Real-time PCR assays for beta-globin and Universal 16S r

133 performance of the RP panel in comparison to real-time PCR assays for the detection of respiratory pa

134 obust, easy-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related sp

135 regions of South Africa were used in duplex real-time PCR assays to simultaneously detect A. margina

136 Therefore, integrated DNA barcodes, Real-Time PCR assays using TaqMan probes and UHPLC-HR-MS

137 The emergence of real-time PCR assays utilizing allele-specific molecular

138 When the ELISAs and real-time PCR assays were applied to the analysis of 15

139 lood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 8

140 ples by in-house nested PCR and quantitative real-time PCR assays, respectively.

141 and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacte

142 RP panel with those of laboratory-developed real-time PCR assays, using a variety of previously coll

143 SAs were found to be more sensitive than the real-time PCR assays.

144 presence of acanthamoebae with four separate real-time PCR assays.

145 EV Ag-specific ELISA was less sensitive than real-time PCR at detection of HEV infection.

146 This multiplex real-time PCR, based on the dual-labeled probe strategy,

147 Two real-time PCR-based assays were developed to assess indu

148 We optimized a DNA extraction and real-time PCR-based method that could reliably detect 1

149 We developed a real-time PCR-based TaqMan array card (TAC) that can tes

150 for TLRs 2, 3, 4, 7, and 9 was quantified by real-time PCR before therapy.

151 pecific ELISA is less sensitive than HEV RNA real-time PCR but represents a useful tool to discrimina

152 ics AsperGenius species assay is a multiplex real-time PCR capable of detecting aspergillosis and gen

153 ulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectroscopy to the mo

154 ive stool samples with reverse transcription-real-time PCR cycle threshold (CT) values of 10 to 43.

155 The real time PCR data for the MMTBI, STBI and orthopedic in

156 by TaqMan array card (TAC), a multipathogen real-time PCR detection platform.

157 d, based on DNA extraction from semolina and real-time PCR determination of T. aestivum in Triticum s

158 were assessed by means of Western blotting, real-time PCR, differentiation, and proliferation assays

159 We then performed real-time PCR followed by high-resolution melting (HRM)

160 t work describes five methodologies based in Real Time PCR for detection of the most relevant fish sp

161 and throat swabs were tested using multiplex real-time PCR for 33 respiratory pathogens (FTD((R)) kit

162 CT recipients between 2004 and 2014, in whom real-time PCR for RV was performed in nasopharyngeal asp

163 The aim of this study was to evaluate a real-time PCR for serotyping S. flexneri and to use whol

164 es retinal lesions (both sexes), by means of real-time PCR for the neuronal activity reporter gene zi

165 d the performances of two recently developed real-time PCR HCV RNA assays, cobas HCV for use on the c

166 of PSCs was performed using flow cytometry, real-time PCR, immunofluorescence staining, confocal mic

167 Western blot, real-time PCR, immunohistochemistry and cell viability a

168 s determined using neutralization assays and real time PCR in BoHV-1 infected Madin-darby bovine kidn

169 M. pneumoniae was detected by real-time PCR in 175 (5.8%) specimens.

170 The fungus was detected using real-time PCR in 26 (8.6%) specimens across the period o

171 the diagnosis of malaria compared to that of real-time PCR in a high-transmission setting.

172 Indeed, we find that the use of real-time PCR in a quality assured clinical laboratory s

173 ic enteropathogens in the same samples using real-time PCR in a Taqman array card (TAC) format.

174 IL-37 and IL-18Rap was measured by ELISA and real-time PCR in genotyped healthy individuals.

175 were detected by toluidine blue staining and real-time PCR in human biopsies and in tumors from athym

176 ination of patch-clamp electrophysiology and real-time PCR in MNCs in sham and renovascular hypertens

177 s of PCa, was not detectable by quantitative real-time PCR in samples from healthy volunteers.

178 S1P-R expression was quantified by means of real-time PCR in sorted thymocyte subsets and flow cytom

179 inducible chemokines were evaluated by using real-time PCR in the liver and spleen and by means of EL

180 entially amplified using RT-LAMP on either a real-time PCR instrument for quantitative analysis, or i

181 Results from this study showed that TaqMan real-time PCR, is potentially an effective method for th

182 We evaluated a laboratory-developed real-time PCR (LD-PCR) assay targeting stx1, stx2, and r

183 Quantitative real-time PCR measurement of parasite DNA in plasma is a

184 n was analyzed by ELISA, flow cytometry, and real-time PCR measurements of cytokines and TH cell tran

185 Real-time PCR measurements of SK channel subunits mRNA i

186 A Taqman-based real time PCR method for the detection of Pinus spp. was

187 For this reason, a multiplex Real Time-PCR method based on TaqMan technology for the

188 species and used, subsequently, to develop a real-time PCR method coupled with HRM analysis.

189 Event- and construct-specific real-time PCR methods for detection of the GM strain and

190 an tissues was analysed through quantitative real-time PCR methods, to quantify the relative amount o

191 tem-loop, reverse transcriptase quantitative real-time PCR miRNA expression profiling (screening coho

192 expression was validated using quantitative real time PCR (n = 21) and western blotting (n = 9).

193 This was confirmed using quantitative real-time PCR (n = 4).

194 sitives and BioThreat-E test negatives and 4 real-time PCR negatives and BioThreat-E test positives),

195 We performed real-time PCR on a subset of subjects who had undergone

196 They conducted quantitative real-time PCR on genomic DNA isolated from tumor and mat

197 copy number was measured using quantitative real-time PCR on PBC DNA samples from participants in th

198 Out of 21 clinical samples tested by real-time PCR, only 1 was positive and 13 were negative

199 Cases were confirmed by culture or direct real-time PCR, or both, of cerebrospinal fluid specimens

200 treatment with highly sensitive quantitative real-time PCR, or MDA with blood-stage treatment alone,

201 condary standards, as did the results of the real-time PCRs, particularly when plotted against nomina

202 g of soybean DNA (8.6 copies), with adequate real-time PCR performance parameters, regardless of the

203 es, down to 0.01pg of fish DNA, and adequate real-time PCR performance parameters.

204 l sensitivity if performed on an appropriate real-time PCR platform.

205 that the SmartCycler II, compared with other real-time PCR platforms, decreases the clinical sensitiv

206 In 9 discrepant results (5 real-time PCR positives and BioThreat-E test negatives a

207 The specificities of the real-time PCR primers were 94.2% for P. vivax (49/52) an

208 More specifically, quantitative real time PCR (qPCR) achieves a high degree of sensitivi

209 ometry, micro-computed tomography (muCT) and real time-PCR (qPCR) analyses, individual trabecula segm

210 gland and this was confirmed by quantitative real-time PCR (qPCR) analysis, suggesting their specific

211 ell lysate with subsequent quantification by real-time PCR (qPCR) analysis.

212 two different DNA amplification techniques, real-time PCR (qPCR) and real-time Loop-mediated isother

213 Here we employed quantitative real-time PCR (qPCR) assays for polyphosphate kinase 1 (

214 We analyzed quantitative real-time PCR (qPCR) assays on DNA extracted from these

215 ificantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly co

216 By quantitative reverse transcription-real-time PCR (qPCR) sapovirus was detected in 12.4% (37

217 (i) classical culture and (ii) quantitative real-time PCR (qPCR) targetinglytAin patients with LRTIs

218 We used quantitative real-time PCR (qPCR) to test for 32 enteropathogens in s

219 during the summer of 2012, and quantitative real-time PCR (qPCR) was used to enumerate several ARGs

220 ific lateral-flow device (LFD), quantitative real-time PCR (qPCR), and the galactomannan (GM) test we

221 ults were also confirmed by the quantitative real-time PCR (qPCR), droplet digital PCR (ddPCR), and S

222 As including northern blotting, quantitative real time PCR (qRT-PCR) and microarray technology beside

223 r expression levels analyzed by quantitative real time PCR (qRT-PCR) at different time points after h

224 s that code identified protein, quantitative real time PCR (qRT-PCR) was performed.

225 Quantitative real-time PCR (qRT-PCR) analysis of selected genes also

226 Two quantitative assays, quantitative real-time PCR (qRT-PCR) and droplet digital PCR (ddPCR),

227 Consistent with these findings, quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent

228 Functional validation using quantitative real-time PCR (qRT-PCR) indicated four promising candida

229 Using quantitative real-time PCR (qRT-PCR) we have measured the gene expres

230 Using quantitative real-time PCR (qRT-PCR), it showed that the DRELFA is ve

231 After adaptation to multiplex quantitative real-time PCR (qRT-PCR), the signature was used to predi

232 cted to microarray analysis and quantitative real-time PCR (qRT-PCR).

233 ion process was validated using quantitative real-time PCR (qRT-PCR).

234 the development of a quadruplex quantitative Real Time PCR (qxPCR) based on SYBR(R)GreenER chemistry,

235 cal laboratory and gave similar results to a real-time PCR reference test with limits of detection of

236 2 prioritized miRNAs were investigated using real-time PCR; relative expression of miRNAs in sperm wa

237 ical examination along with Western blot and real-time PCR, respectively.

238 ues such as polymerase chain reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have a

239 available genomic data to evaluate a duplex real-time PCR (RT-PCR) assay that targets mapA and ceuE

240 Rapid real-time PCR (RT-PCR) can be performed in a community h

241 A quantitative real-time PCR (RT-PCR) method, employing novel primer se

242 eat mixtures using both conventional PCR and real-time PCR (RT-PCR).

243 d were compared with two genotyping methods (real-time PCR [rt-PCR] and whole-genome sequencing [WGS]

244 isolates, we demonstrate concordance between real-time PCR serotyping and latex agglutination.

245 We propose that real-time PCR serotyping represents an attractive altern

246 High-throughput sequencing and quantitative real-time PCR showed a significant compositional shift,

247 Here, microarray analysis and quantitative real-time PCR showed that miR-365 was robustly decreased

248 Real-Time PCR showed to be suitable for detection of foo

249 Real-time PCR studies indicated that the transcriptional

250 PCR system [Stratagene-Agilent] and the 7500 real-time PCR system [ABI Life Technologies]).

251 posing a novel specific and highly sensitive real-time PCR system for the detection/quantification of

252 system and the Applied Biosystems (ABI) 7500 real-time PCR system.

253 In this work, two real-time PCR systems based on the EvaGreen dye and a Ta

254 his work describes the development of a new, real-time PCR TaqMan assay for the detection of ling (Mo

255 Syrian hamsters by a sensitive quantitative real-time PCR (TaqMan) with lipl32 as the target gene.

256 for the identification of H. haemolyticus A real-time PCR targeting purT (encoding phosphoribosylgly

257 ish DNA barcoding, species-specific PCR, and real-time PCR techniques for the identification of six s

258 d clinical specimens compared to a validated real-time PCR test.

259 o allows extension to integrate with in situ real-time PCR thermal cycling since the sample slide is

260 We used quantitative real-time PCR to determine DEFA1A3 DNA copy numbers in 2

261 next-generation sequencing and quantitative real-time PCR to determine the impact of dry cow therapy

262 University, where HIV DNA was assessed with real-time PCR to establish HIV infection.

263 We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus ga

264 an cortical development we used quantitative real-time PCR to examine the expression trajectories of

265 rthermore, we used cycle threshold values of real-time PCR to guide the choice of protocols for SNP a

266 ere validated empirically using quantitative real-time PCR to measure gene expression.

267 t can unite the reward of multiplex PCR with real-time PCR to recognize animal genes rapidly in feeds

268 We validated these results by quantitative real-time-PCR using NBUVB-treated vitiligo skin and untr

269 asmonic photothermal method for quantitative real-time PCR, using gold bipyramids and light to achiev

270 anti-HEV Ag-ELISA was compared with that of real-time PCR, using sera from a cohort of acutely infec

271 Quantitative real-time PCR was employed to validate candidate genes.

272 Real-time PCR was performed for gene expression analysis

273 Quantitative real-time PCR was used to show that KOR and prodynorphin

274 A multiplex real-time PCR was validated on the BD Max open system to

275 Assessing mRNA of SR-B1 by real time PCR we found messenger expression in LSEC to b

276 niques (clone libraries, pyrosequencing, and real-time PCR), we show that polymetallic nodules provid

277 Using real time PCR, we found PARs to be expressed in smooth m

278 Using immunohistochemistry and quantitative real-time PCR, we assessed biopsy specimens from 19 chil

279 Using quantitative real-time PCR, we showed that the block to infection occ

280 two-step algorithm compared to results with real-time PCR were 95.5% (95% CI, 90.5 to 98.0%) and 91.

281 cificity of the RDT compared to results with real-time PCR were 99.4% (95% confidence interval [CI],

282 n additional 7 samples that were negative by real-time PCR were positive with T2MR.

283 on the DNA of fish (Oncorhynchus mykiss) by real-time PCR were studied.

284 Microbiological culture and real-time PCR were used as gold standards.

285 Western blotting and Real-time PCR were used to assess the expressions of BDN

286 Microarray analysis and real-time PCR were used to examine transcriptional diffe

287 Real time PCR, western blot and knock down assays demons

288 nt times during the regenerative process, by real time PCR, Western blotting analysis, and immunohist

289 d localization were assessed by quantitative real-time PCR, Western blot analysis, and immunohistoche

290 sessed using luciferase assays, quantitative real-time PCR, western blots, scratch assays, CCK-8 assa

291 MUC4 and MUC4beta was evaluated by means of real-time PCR, Western blotting, and immunohistochemistr

292 ression of MUC1 and MUC1 CT was evaluated by real-time PCR, Western blotting, and immunohistochemistr

293 proaches, including microarray, quantitative real-time PCR, Western blotting, chromatin immunoprecipi

294 d cell sorting, RNA sequencing, quantitative real-time PCR, Western blotting, small interfering RNA i

295 ichment step with high resolution melt (HRM) real-time PCR which is a sensitive assay with a rapid ti

296 m most of the case patients were positive by real-time PCR, while most of the subsequently collected

297 amily member D) was lower in NF1-iN cells by real-time PCR with 12 sex-mixed samples.

298 t RNRf/RNRr was designed and evaluated using real-time PCR with 262 HLB samples collected from China

299 Species-specific PCR and real-time PCR with EvaGreen dye targeting the ITS region

300 /ml) was detected in FCDI cell lysates using real-time PCR with greater consistency than with prepara