ライフサイエンスコーパス: real-time quantitative PCR

1 and 2 up-regulated genes) were validated by real time quantitative PCR.

2 iral vector to other organs was evaluated by real time quantitative PCR.

3 PTGS2, CXCR1, and PTK2 were determined using real-time quantitative PCR.

4 ication in human beta-cells was performed by real-time quantitative PCR.

5 1), differential expression was confirmed by real-time quantitative PCR.

6 (promote N-glycan branching) as detected by real-time quantitative PCR.

7 as in CD4+CD8- single-positive thymocytes by real-time quantitative PCR.

8 were confirmed in microdissected samples by real-time quantitative PCR.

9 t and 3 and 7 days later, was analyzed using real-time quantitative PCR.

10 east 10-fold more than the other isoforms by real-time quantitative PCR.

11 individual infection load then estimated by real-time quantitative PCR.

12 of these two methods were then validated by real-time quantitative PCR.

13 remodeling, respectively) was quantified by real-time quantitative PCR.

14 ded the peak levels of CCL17, as measured by real-time quantitative PCR.

15 e blood recall assays, HLA-A2 tetramers, and real-time quantitative PCR.

16 in lung tissue of bleomycin-treated mice by real-time quantitative PCR.

17 expression were subsequently confirmed using real-time quantitative PCR.

18 The load of infection was then estimated by real-time quantitative PCR.

19 communities in Tanzania and The Gambia with real-time quantitative PCR.

20 ome fetuses and five independent controls by real-time quantitative PCR.

21 sion of some of these genes was confirmed by real-time quantitative PCR.

22 anglia, as demonstrated by virus culture and real-time quantitative PCR.

23 CCDC134, UBD, and ZIC2 were validated using real-time quantitative PCR.

24 wing abnormal expression were validated with real-time quantitative PCR.

25 All patients were monitored by real-time quantitative PCR.

26 ormances and reaching similar sensitivity as real-time quantitative PCR.

27 ntification of the repaired, CPD-free DNA by real-time quantitative PCR.

28 an innate, immune response was quantified by real-time quantitative PCR.

29 Relative MCP-1 mRNA was measured by real-time quantitative PCR.

30 tern blot or harboring HDV RNA detectable by real-time quantitative PCR.

31 MRD was measured by using standardized real-time quantitative PCR.

32 elongs to the same cluster, was confirmed by real-time quantitative PCR.

33 e different cultured cells was validated via real-time quantitative-PCR.

34 cycling, making this method well suited for real-time quantitative PCRs.

35 By the use of real-time quantitative PCR, a 10-fold increase in MT-1 a

36 antified by dilution to single molecules and real-time quantitative PCR amplification.

37 Real time quantitative PCR analyses showed that unlike i

38 Real-time quantitative PCR analyses of Jurkat cell infec

39 In this paper, we report extensive real-time quantitative PCR analyses of SIX3 and SIX6 exp

40 Real-time quantitative PCR analyses showed that the expr

41 at are impaired in the resistant cell lines, real-time quantitative PCR analyses were employed and tw

42 Real time quantitative PCR analysis demonstrates that th

43 Real-time quantitative PCR analysis demonstrates that th

44 Real-time quantitative PCR analysis demonstrates TuSp1 i

45 Real-time quantitative PCR analysis indicated that sever

46 Real-time quantitative PCR analysis of a set of cancer-r

47 Real-time quantitative PCR analysis of gene expression r

48 However, our real-time quantitative PCR analysis revealed significant

49 Real-time quantitative PCR analysis shows that PDEs 9A5

50 enome hybridization (aCGH) followed by rapid real-time quantitative PCR analysis to identify, confirm

51 Using microarrays and real-time quantitative PCR analysis, we defined differen

52 dition, the TRAP assay has been modified for real-time, quantitative PCR analysis.

53 as carried out on a subset of 66 clones with real time quantitative PCR and 40 clones were positive.

54 Real time quantitative PCR and Western blot analyses wer

55 seI and Trap1 mRNA levels were determined by real-time quantitative PCR and compared with protein exp

56 Using real-time quantitative PCR and cotransfection reporter a

57 sion varied considerably as measured by both real-time quantitative PCR and flow cytometry analysis.

58 ling using microarray analysis combined with real-time quantitative PCR and flow cytometry.

59 re highly upregulated or downregulated using real-time quantitative PCR and found a strong correlatio

60 The increase was corroborated by real-time quantitative PCR and immunofluorescence.

61 TJ expression was evaluated by using real-time quantitative PCR and immunofluorescence.

62 Using real-time quantitative PCR and immunohistochemical metho

63 vo gene expression profiles were verified by real-time quantitative PCR and immunohistochemistry.

64 Real-time quantitative PCR and in situ hybridization ana

65 Real-time quantitative PCR and in situ hybridization val

66 Using real-time quantitative PCR and in situ hybridization, we

67 Validating these findings by real-time quantitative PCR and layered protein scanning,

68 Real-time quantitative PCR and Northern analyses reveale

69 We have determined, by real-time quantitative PCR and RNase protection, that th

70 of candidate genes by reverse transcriptase real-time quantitative PCR and subsequent testing for ak

71 expression patterns of mouse macrophages by real-time quantitative PCR and tested the functional eff

72 Real-time quantitative PCR and Western blot analysis dem

73 On the basis of real-time quantitative PCR and Western blot analysis, ex

74 irmed in splenocytes and total thymocytes by real-time quantitative PCR and Western blot as well as i

75 human epidermal keratinocytes (n = 11) with real-time quantitative PCR and Western blotting revealed

76 genes in the region was carried out by using real-time quantitative PCR and/or oligo-microarray profi

77 n and gene transcript levels (gene array and real-time quantitative PCR) and compared with insulin-tr

78 n of IL-13Ralpha2 mRNA was measured by using real-time quantitative PCR, and cell-surface IL-13Ralpha

79 ds for islet expansion using immunostaining, real-time quantitative PCR, and microarrays at the follo

80 Our in situ hybridization, real-time quantitative PCR, and stage-specific transcrip

81 mutant TIP30, by Affymetrix GeneChip array, real-time quantitative PCR, and Western blotting assays

82 cts has been utilized extensively in various real-time quantitative PCR applications, including post-

83 g conventional reverse transcriptase-PCR and real-time quantitative PCR, as well as whole-mount in si

84 bjects from 3 independent cohorts by using a real-time quantitative PCR assay to detect a noninherite

85 growth factor (EGF) mRNA were measured using real-time quantitative PCR assay, and levels were correl

86 yzed mRNA expression with a highly sensitive real-time quantitative PCR assay.

87 nd by infected ticks as evaluated by using a real-time quantitative PCR assay.

88 were tested for HIV shedding and VL using a real-time quantitative PCR assay.

89 gand evolution was assured by a differential real-time quantitative PCR assay.

90 in 18 biopsies (10 IFTA and 8 normal) using real-time quantitative PCR assays (RT-QPCR).

91 estimate infection prevalence, we developed real-time quantitative PCR assays for each virus and scr

92 Sensitive qualitative and real-time quantitative PCR assays revealed a higher prev

93 Using real-time quantitative PCR assays to study SXT excision

94 Results of real-time quantitative PCR assays, however, indicated th

95 Real-time quantitative PCR assays, immunohistochemistry,

96 Using real-time quantitative PCR assays, mRNA levels of ERalph

97 using traditional plaque assays, as well as real-time quantitative PCR-based genome quantification a

98 sured by a highly sensitive and quantitative real-time quantitative-PCR-based telomeric repeat amplif

99 lymphocytes' telomere length was measured by real-time quantitative PCR before HSCT.

100 istry, Western blotting, DNA binding assays, real-time quantitative PCR, coimmunoprecipitation, and E

101 Real Time quantitative PCR confirmed that gene expressio

102 Real time quantitative PCR confirmed up-regulation of Eg

103 Real-time quantitative PCR confirmed expression patterns

104 Real-time quantitative PCR confirmed that CpG ODN decrea

105 Real-time quantitative PCR confirmed that IL9 was the hi

106 Real time quantitative PCR demonstrated an increase in F

107 More sensitive real-time quantitative PCR detected AID transcripts in v

108 Real-time quantitative PCR detected mRNAs for CD8(+) T-c

109 principle, we demonstrate an electrochemical real-time quantitative PCR (e-PCR) measurement in the to

110 microscopy, cocultures, suppression assays, real-time quantitative PCR, ELISAs, and ELISpot assays w

111 portions were subjected to mRNA extraction, real-time quantitative PCR, enzyme-linked immunosorbent

112 "Minimum Information for the publication of real-time Quantitative PCR Experiments" (MIQE) guideline

113 The allelic variation has been confirmed by real-time quantitative PCR experiments.

114 pression of candidate genes was confirmed by real-time quantitative PCR, fluorescence, and immunohist

115 Plaque and saliva samples were tested with real-time quantitative PCR for DNA levels of pathogens r

116 ra-1 gene silenced RPM cells were assayed by real-time quantitative PCR for the expression of possibl

117 Using real-time quantitative PCR for the quantification of gen

118 ce and levels of male DNA were determined by real-time quantitative PCR for the Y chromosome-specific

119 Microarray findings were confirmed by using real-time quantitative PCR in 30 subjects (12 children w

120 ssion of plasma miRNAs was measured by using real-time quantitative PCR in 35 asthmatic patients, 25

121 chromosome-specific DYS14 gene, we performed real-time quantitative PCR in autopsied brain from women

122 erated, and their expression was measured by real-time quantitative PCR in blood samples from 81 meta

123 aggression testing at both time points using real-time quantitative PCR in brain regions previously i

124 IL-34 mRNA levels were quantified by real-time quantitative PCR in human synovial fibroblasts

125 expression of all known chemokine ligands by real-time quantitative PCR in isolated islets.

126 ional Scale (BCR-ABL(IS)) of 0.1% or less by real-time quantitative PCR in peripheral blood.

127 We analyzed XDH1 and XDH2 gene expression by real-time quantitative PCR in tissues from sugar- and bl

128 We used real-time quantitative PCR, in situ nucleic acid hybridi

129 Analysis of gene expression by real time quantitative PCR indicated that whitefly morta

130 Real-time quantitative PCR is extremely accurate and les

131 Real-time quantitative PCR is used routinely for the hig

132 ects of this deficiency were demonstrated by real-time quantitative PCR measurements of altered mRNA

133 We have developed a novel "real time" quantitative PCR method.

134 immunoprecipitation, promoter mutations, and real-time quantitative PCR, NRF-1 was found to functiona

135 ipitation, promoter mutational analysis, and real-time quantitative PCR, NRF-1 was found to functiona

136 orts to standardize MRD quantification using real-time quantitative PCR of clonal immunoglobulin and

137 ical rates in cell culture, as determined by real-time quantitative PCR of viral particles released i

138 F-beta, IL-5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of pati

139 sence or absence of certain antibiotics with real-time quantitative PCR or digital PCR to determine a

140 e tissue using Western blot, flow cytometry, real-time quantitative PCR, or RNA sequencing analyses.

141 itionally, we applied a recently established real-time quantitative PCR platform to gain insight into

142 Next generation RNA sequencing of mRNAs and real time quantitative PCR profiling established that Ez

143 Real-time quantitative PCR provided a sensitive and reli

144 We evaluate here the use of real-time quantitative PCR (q-PCR) as a method for scree

145 Expression of IL-17 was determined by real-time quantitative PCR (qPCR) after 5 h and protein

146 ta combining transcriptional lacZ fusion and real-time quantitative PCR (qPCR) analyses indicated tha

147 ive genomic hybridization (CGH) database and real-time quantitative PCR (qPCR) analyses to identify t

148 Here we describe a novel real-time quantitative PCR (qPCR) approach based on the

149 We report the first real-time quantitative PCR (qPCR) assay targeting Asperg

150 rformance of the Xpert Carba-R test, a rapid real-time quantitative PCR (qPCR) assay that detects fiv

151 To this end, we developed type-specific real-time quantitative PCR (qPCR) assays for HAdV types

152 We developed two real-time quantitative PCR (qPCR) assays, targeting the

153 Real-time quantitative PCR (qPCR) is one of the most pow

154 riance across the lake as fluorescence based real-time quantitative PCR (qPCR) measurements of microc

155 Interestingly, real-time quantitative PCR (qPCR) on E2F2 ChIPs indicate

156 Real-time quantitative PCR (qPCR) was used to measure mR

157 When measured by comparative real-time quantitative PCR (qPCR), HP1512 transcription

158 ples whose parasite burdens were measured by real-time quantitative PCR (qPCR).

159 ucleotides hybridized to a miRNA followed by real-time quantitative PCR (qPCR).

160 and a greater resistance to inhibitors than real-time quantitative PCR (qPCR).

161 r the molecular detection of the organism by real-time quantitative PCRs (qPCRs) targeting the uracil

162 ononuclear cells was screened by nested- and real time-quantitative PCR (QRT-PCR) for the presence of

163 chain reaction (ddPCR) is rapidly replacing real-time quantitative PCR (qRT-PCR) as an efficient met

164 Real-time quantitative PCR (qRT-PCR) confirmed the resul

165 Using SnoRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoR

166 nin-concentrating hormone, as revealed using real-time quantitative PCR, radiolabeled in situ hybridi

167 tification of the transcripts by RNA-Seq and real time quantitative PCR revealed that the CYP3A4 tran

168 The use of cDNA microarrays and real-time quantitative PCR revealed increased expression

169 ypothalamic peptides via radioimmunoassay or real-time quantitative PCR revealed markedly enhanced ga

170 Real-time quantitative PCR revealed no transcripts in ne

171 Real-time quantitative PCR revealed that 96% of the ampl

172 Progress includes establishment of optimized real-time quantitative PCR (RQ-PCR) assays for WT1 (comm

173 now possible using updated methods including real-time quantitative PCR (RQ-PCR) for abnormal fusion

174 before and after the 12-week treatment using real-time quantitative PCR (RT-qPCR) analysis.

175 chanism of how Dam and GidA influence Act, a real-time quantitative PCR (RT-qPCR) assay was performed

176 Western blot, real-time quantitative PCR (RT-qPCR), and transcriptiona

177 oter cloning, and site-directed mutagenesis, real-time quantitative PCR (RT-qPCR), and Western blotti

178 expression on prostate cancer cell lines by real-time quantitative PCR (RT-qPCR), flow cytometry, an

179 Real-time quantitative PCR (RT-qPCR), single-molecule in

180 = 14) and in 7 healthy male volunteers using real-time quantitative PCR (RT-qPCR).

181 CR) and AUC, 0.996 (95% CI, 0.993-0.998) for real-time quantitative PCR (RTQ-PCR) (P = .02).

182 Real-time quantitative PCR (RTQ-PCR) provides a quick, a

183 mples were tested for cytomegalovirus DNA by real-time quantitative PCR (rtqPCR).

184 Real-time quantitative PCR showed that BAC-IN84/Ep was d

185 ern blot data, reverse transcription-PCR and real-time quantitative PCR showed that gp340 transcripts

186 Real-time quantitative PCR shows that Afralea3m mRNA is

187 In real-time quantitative PCR studies using absolute plasmi

188 Using gene chip analysis and the real-time quantitative PCR system, we measured transcrip

189 Real-time quantitative PCR systems (Q-PCR) for the rapid

190 Real-time quantitative PCR techniques were used to deter

191 We develop a real-time quantitative PCR telomeric repeat amplificatio

192 Real-time quantitative PCR tests showed elevated levels

193 study, we demonstrate by deep sequencing and real-time quantitative PCR that hepatic levels of Foxa2

194 We developed real-time quantitative PCRs that allow reliable differen

195 By using real-time quantitative PCR, the population dynamics and

196 viral loads of exposed fish, measured using real-time quantitative PCR; the most virulent viral stra

197 to assess metabolic reactions and fluxes and real time quantitative PCR to determine gene expression.

198 We used real-time quantitative PCR to analyze mtDNA integrity, d

199 robotics, multiparameter flow cytometry, and real-time quantitative PCR to analyze T cell activation

200 Using real-time quantitative PCR to detect endogenous GPNMB ex

201 tions of limbal epithelium were subjected to real-time quantitative PCR to determine c-kit ligand exp

202 ne amplification with message level, we used real-time quantitative PCR to measure hTERT mRNA in 50 e

203 We used real-time quantitative PCR to measure Treponema pallidum

204 -denaturing gradient gel electrophoresis and real-time quantitative PCR to monitor and quantify chang

205 opy, semithin sectioning of leaf tissue, and real-time quantitative PCR to study structural and quant

206 Real-time quantitative PCR using DNA from the animal who

207 e, 14 human bladder tumours were analysed by real-time quantitative PCR using gene-specific primers f

208 array results with the reverse transcription real-time quantitative PCR, using a larger number of non

209 Real Time-quantitative PCR was performed for SERPINA3 tr

210 Real-time quantitative PCR was also used to measure the

211 Real-time quantitative PCR was performed to assess cytok

212 production was measured by using ELISA, and real-time quantitative PCR was performed to detect PGE2

213 Real-time quantitative PCR was performed to determine th

214 Real-time quantitative PCR was used to analyze the expre

215 Real-time quantitative PCR was used to quantitate LYN, S

216 In addition, real-time-quantitative PCR was utilized to assess mRNA l

217 Using RT(2) Profiler PCR Array and real-time quantitative PCR we found several important sy

218 Using real-time quantitative PCR, we demonstrated that several

219 Using real-time quantitative PCR, we establish that hypothalam

220 Using immunocytochemistry and real-time quantitative PCR, we found that winning fights

221 Using real-time quantitative PCR, we found that within 1 h of

222 te and sensitive assay of parasite genotype, real-time quantitative PCR, we have investigated protect

223 Using DNA microarrays and real-time quantitative PCR, we identified genes transcri

224 Using ELISA as well as real-time quantitative PCR, we show that cultured primar

225 Using allele-specific real-time quantitative PCR, we tested the hypotheses tha

226 Microtomography, histology, and real-time quantitative PCR were performed to analyze bon

227 , and mRNA expression levels (measured using real-time quantitative-PCR) were related to fetal and ne

228 tissue samples from lung cancer patients by real-time quantitative PCR, Western blot, and IHC and fo

229 changes of gene expression were confirmed by real-time quantitative PCR, Western blotting, and functi

230 This finding was confirmed by real-time quantitative PCR, which also demonstrated an a

231 ion of these genes, 7 of them were tested by real-time quantitative PCR, which verified that they wer

232 Real time quantitative PCR with Mesa Green, targeted at

233 n reaction (PCR) enzymes were compared using real-time quantitative PCR with SYBR Green I detection.

234 The assay combines kinetic (real-time quantitative) PCR with allele-specific amplifi