ライフサイエンスコーパス: reassortant virus

1 f the PR8 NS gene for that of a Vero-adapted reassortant virus.

2 salts was also mapped to L3/lambda1 by using reassortant viruses.

3 resulting in new genetic constellations and reassortant viruses.

4 raminidase activity and virion morphology of reassortant viruses.

5 tions also strongly affected the genotype of reassortant viruses.

6 the viability and replicative ability of the reassortant viruses.

7 s segments to diminish their ability to form reassortant viruses.

8 also contributed to the pathogenesis of the reassortant viruses.

9 igs with reverse genetics-created HA plus NA reassortant viruses.

10 e wholly Eurasian lineage H5N8 and two novel reassortant viruses.

11 he time of onset of virus shedding in triple-reassortant viruses.

12 influenza virus (A/Memphis/31/98, H3N2) in a reassortant virus A/NWS/33(HA)-A/Mem/31/98(NA) (H1N2) an

13 ray analysis with a high infectivity "triple reassortant" virus ((A/Swine/MN/593/99 (H3N2)) that spre

14 Infection with the NP reassortant virus also resulted in the upregulation of g

15 of these amino acid changes into an H7N9 PR8 reassortant virus also significantly improved viral tite

16 Exemplar reassortant viruses also replicated to similar titers in

17 While both 1918 reassortant viruses also were highly pathogenic, the H5N

18 ween the capacity of T1L x T3A and T1L x T3D reassortant viruses and field isolate strains to inhibit

19 surface protein mu1 by genetic analyses with reassortant viruses and recoated cores.

20 A genetic study with reassortant viruses and subsequent biochemical analyses

21 ole for the RNP complex in the generation of reassortant viruses, and a functional interaction of PB2

22 athogenicity avian H7N3 virus and that these reassortant viruses are stable and not attenuated in an

23 th pandemic potential, including avian-human reassortant viruses as tested here, may emerge.

24 From studies with single-gene reassortant viruses bearing one gene segment from the AA

25 th the 1918 and 1918HA/K173 viruses, whereas reassortant viruses bearing the 1918 viral RNA polymeras

26 es, we generated all the 254 combinations of reassortant viruses between A/chicken/South Kalimantan/U

27 m for the generation of single and quadruple reassortant viruses between A/Teal/HK/W312/97 (H6N1) and

28 To evaluate the pathogenic potential of reassortant viruses between currently cocirculating avia

29 the context of single-, double-, and triple-reassortant viruses between PR8 and SPN04; PR8/SPN04 M,

30 re, we examined the biological properties of reassortant viruses between the 1918 virus and a contemp

31 ability to enhance the pathogenicity of the reassortant viruses but could do so by cooperating with

32 ed the functionality of the H7N3:PH1N1 7 + 1 reassortant viruses by measuring the inflammatory respon

33 We created otherwise isogenic reassortant viruses by site-directed mutagenesis that co

34 w that following adaptation in the ferret, a reassortant virus carrying the surface proteins of an av

35 Finally, we show that reassortant viruses carrying gene segments of CIV and hI

36 Previously, we showed that a reassortant virus composed of wild-type avian H9N2 surfa

37 f infection with HPAI (the most virulent), a reassortant virus containing 1918 hemagglutinin and neur

38 In contrast, a reassortant virus containing an oseltamivir-resistant se

39 ere isolated from infected animals: a double-reassortant virus containing genes similar to those of h

40 ose of human and swine viruses, and a triple-reassortant virus containing genes similar to those of h

41 logenetic analyses show that IND1982/01 is a reassortant virus containing genome segments derived fro

42 Here, we evaluated if a reassortant virus containing the hemagglutinin and neura

43 The reassortant virus containing the original wt A/Anhui/1/2

44 We also used reverse genetics to generate a reassortant virus containing the PB1 gene of the A/PR/8/

45 s of IAV have resulted in the circulation of reassortant viruses containing human-origin genes in pig

46 the spread of virus to the MLN (P = 0.02) in reassortant viruses containing segment 7 from the spread

47 whether transmissible H5 subtype human-avian reassortant viruses could be generated in vivo.

48 humans, raising the concern that H9N2:pH1N1 reassortant viruses could emerge.

49 Studies with reassortant viruses demonstrated that expression of the

50 In assays using a panel of reassortant viruses derived from these strains, the diff

51 man lung epithelial A549 cells (however, the reassortant virus did not replicate more efficiently tha

52 A reassortant virus (Dk78/Eng62N2) did not propagate in du

53 rograms to monitor the emergence of human H5 reassortant viruses, especially those containing a PB2 s

54 A reassortant virus expressing only the HA and neuraminida

55 A reassortant virus expressing the 1918 viral RNA polymera

56 In reassortant viruses, factory costaining for cUb mapped p

57 ial clinical implications of the avian-swine reassortant virus for pathogenicity in pigs or other spe

58 Given the relatively high incidence of reassortant viruses from tissues of the ferret upper air

59 AC resistance of reassortant viruses generated from a cross of wt type 1

60 reased infectivity, using laboratory-derived reassortant viruses generated from sialic acid binding r

61 Two antigenically distinct reassortant viruses (H3N2) were isolated from infected a

62 The spread of H5N8 and the novel reassortant viruses, H5N2 and H5N1 (H5Nx), in domestic p

63 This reassortant virus had a higher titer and total protein y

64 Nineteen of these reassortant viruses had high-growth phenotypes in vitro,

65 SIVs) into U.S. swine in 1998, H1N2 and H1N1 reassortant viruses have emerged from reassortment betwe

66 e HA of human A/Udorn/307/72 (H3N2) permit a reassortant virus [human Udorn HA, with all other genes

67 The inclusion phenotypes of T1L x T3D reassortant viruses identified the viral M1 genome segme

68 A genetic analysis using T1L x T3D reassortant viruses implicated the L3 and M1 gene segmen

69 etics of the wholly human virus and a triple-reassortant virus in 7-week-old pigs that were infected

70 y, growth kinetics, and pathogenicity of the reassortant virus in mice.

71 e of Ngari virus, emphasizing a role of this reassortant virus in severe human illness throughout Eas

72 Growth of reassortant viruses in ammonium chloride-treated cells s

73 o exchange genetic sequences and produce new reassortant viruses in swine.

74 Growth of reassortant viruses in the presence of E64 segregated ex

75 Growth of reassortant viruses in the presence of E64 segregated wi

76 The neuraminidase of the reassortant viruses is most closely related to that of B

77 Analysis of reassortant viruses isolated from crosses of an MA mutan

78 We used reassortant viruses isolated from crosses of wild-type (

79 the presence of E64 were identified by using reassortant viruses isolated from independent crosses of

80 PI viruses in the presence of E64, we tested reassortant viruses isolated from independent crosses of

81 s, and propose that the 1918 virus was not a reassortant virus (like those of the 1957 and 1968 pande

82 tween this wholly human virus and the triple-reassortant viruses may affect their replication efficie

83 gglutinin (HA) binding and NA specificity in reassortant viruses may be related to the emergence of n

84 the A(H1N1)pdm09 influenza virus, a complex reassortant virus of swine origin, highlighted the impor

85 d species, with interspecies transmission of reassortant viruses peaking during the winter.

86 Approximately 34% of the recovered reassortant viruses possessed the H5 hemagglutinin (HA)

87 are a 2004 human H5N1 Vietnam isolate with 2 reassortant viruses possessing the 1918 hemagglutinin (H

88 The transmissible H5 reassortant virus preferentially recognized human-type r

89 The reassortant viruses replicated relatively poorly in embr

90 Reassortant viruses represented by A/Chicken/Hong Kong/G

91 Analysis of T1L x T3D reassortant viruses revealed that the mu1-encoding M2 ge

92 in vivo evaluation of the H7N3:PH1N1 (7 + 1) reassortant viruses revealed that the PB2, NA, or M segm

93 Reassortant virus S7, which was unable to reach the live

94 An experiment with genetic reassortant viruses showed that in newborn mice the M se

95 We demonstrate that a reassortant virus that expresses the M2 gene-encoded mu1

96 Here, we show that three H3N2 reassortant viruses that contain 3 (NP, M, and NS) or 5

97 EA virus and a pdm/09 virus yielded 55 novel reassortant viruses that could be categorized into 17 ge

98 st this possibility, we generated a panel of reassortant viruses that expressed the NA genes of human

99 increases the risk of generating H5 subtype reassortant viruses that may be shed from upper airway s

100 growth in cured cells, we used T1L X PI 3-1 reassortant viruses to genetically map the capacity of t

101 a series of human 1918-avian H1N1 influenza reassortant viruses to identify the genetic determinants

102 We used T1L x T3A and T1L x T3D reassortant viruses to identify viral genes associated w

103 PI 2A1 sigma1 protein, we used T1L X PI 2A1 reassortant viruses to identify viral genes associated w

104 The capacity for different reovirus reassortant viruses to induce acute myocarditis in mice

105 e capacities of both T1L x T3A and T1L x T3D reassortant viruses to inhibit cellular DNA synthesis an

106 nsfection-based inoculation system to select reassortant viruses under in vivo selective pressure.

107 -adapted (ca) influenza A/Kawasaki/86 (H1N1) reassortant virus vaccine given with or apart from child

108 In addition, infection with the triple-reassortant virus was associated with moderate to severe

109 Replication capacity of the individual 1:7 reassortant viruses was assessed in mouse lungs and huma

110 that the internal gene complex of the triple-reassortant viruses was associated with three recent phy

111 tify factors that restrict the generation of reassortant viruses, we cotransfected human embryonic ki

112 To investigate the virulence of pH1N1-H5N1 reassortant viruses, we created pH1N1 (A/California/04/2

113 By using T1L x T3D reassortant viruses, we found that differences in the ca

114 Using T1L x T3D reassortant viruses, we found that strain-specific diffe

115 Using reassortant viruses, we showed that differences in the p

116 Two reassortant viruses were assessed in ferrets and showed

117 umber of clade 1.1.2 and 2.3.2.1c interclade reassortant viruses were detected with different combina

118 H3N2 virus backgrounds and found that these reassortant viruses were fully competent in mammalian ho

119 We show here that multiple reassortant viruses were generated during natural coinfe

120 Four reassortant viruses were generated, with three of them s

121 These chicken reassortant viruses were highly similar to the human iso

122 lowing the TRIG cassette emergence, numerous reassortant viruses were isolated in nature, suggesting

123 Most of reassortant viruses were more pathogenic and contagious

124 n (HA), we found that 51 of the 127 possible reassortant viruses were viable and able to be rescued.

125 B2, and NP genes impacted replication of the reassortant viruses while also affecting the expression

126 In this model, an H3N2 reassortant virus with avian virus internal protein gene

127 rissa virus) suggested that it was a genetic reassortant virus with S and L segments derived from Bun

128 Reassortant viruses with avian polymerases and human PA

129 es in pigs, which could give rise to progeny reassortant viruses with enhanced virulence and transmis

130 n but inefficient transmission, whereas H5N1 reassortant viruses with four or six human virus interna

131 ted in an increase in the virus yield of the reassortant viruses without a significant loss of NA act