ライフサイエンスコーパス: recombinant protein

1 tinal epithelial cells by using an Fc-tagged recombinant protein.

2 th the Ser596-containing parasite-derived or recombinant protein.

3 erogeneity present on the IL9 portion of the recombinant protein.

4 H2 clones and Fel d 1 peptide or the Fel d 1 recombinant protein.

5 genesis phenotype by supplementation of DLL4 recombinant protein.

6 parasite isolate was cloned and expressed as recombinant protein.

7 al delivery or through systemic injection of recombinant protein.

8 f a fluorescent dye or exogenously expressed recombinant protein.

9 aggregates in cell lines and of the isolated recombinant protein.

10 ranslation system to create a Sep-containing recombinant protein.

11 ompletely abolished the GPAT activity of the recombinant protein.

12 ins using rabbits antisera to the respective recombinant proteins.

13 rs using WNT3A and endothelin-1 (EDN1) human recombinant proteins.

14 ch was confirmed by coimmunoprecipitation of recombinant proteins.

15 gulin 1 (NRG1) and nerve growth factor (NGF) recombinant proteins.

16 ried out, both in host cells and on purified recombinant proteins.

17 the enzyme sortase A, can be used to modify recombinant proteins.

18 were cloned, overexpressed, and purified as recombinant proteins.

19 haracterized by using hepatic microsomes and recombinant proteins.

20 ipulation of lamin levels by the addition of recombinant proteins.

21 optimized length (tMel-tag) was fused to two recombinant proteins.

22 ing used as an expression system for complex recombinant proteins.

23 r the production of therapeutically relevant recombinant proteins.

24 inetics of Cryptosporidium parvum ACSs using recombinant proteins.

25 ation of tunable functionality into chimeric recombinant proteins.

26 and functionally characterized the purified recombinant proteins.

27 -tag interactions to prepare highly purified recombinant proteins.

28 trilotriacetic acid affinity purification of recombinant proteins.

29 ave been developed as bioreactors to produce recombinant proteins.

30 used them to control the expression level of recombinant proteins.

31 the affinity chromatography purification of recombinant proteins.

32 domains of both human DUOXs and purified the recombinant proteins.

33 G, Fc region specific antibodies or various recombinant proteins.

34 for manufacturing of synthetic peptides and recombinant proteins.

35 authentic posttranslational modifications in recombinant proteins.

36 exameric archaeal MCM in vitro with purified recombinant proteins.

37 only cleave RNA, at least when assayed with recombinant proteins.

38 cosylation also depends on the nature of the recombinant proteins.

39 sing their specific-inhibitors and exogenous recombinant-proteins.

40 mical assays demonstrated that both purified recombinant proteins abolished DNA helicase activity and

41 erexpression or local delivery of human CTGF recombinant protein accelerated bridging and functional

42 ransgenic mouse brain and in vitro assembled recombinant protein account for the greater seeding pote

43 to our algal expression strains give rise to recombinant protein accumulation levels of up to 0.25% o

44 We demonstrate that immunization with a recombinant protein Ag and GLA-SE also induces granzyme

45 The recombinant protein allowed monitoring of the autocataly

46 the generation and isolation of the required recombinant protein alpha-thioesters remain challenging.

47 thod for the preparation and purification of recombinant protein alpha-thioesters using engineered ve

48 Attachment of the affibody molecules to a recombinant protein, already engineered for increased ha

49 , share the same enzymatic properties as the recombinant protein and cleaves RNA with inosine but not

50 ith other immunization strategies, including recombinant protein and DNA.

51 al structure with solution state analysis of recombinant protein and electron cryo-microscopy of acid

52 )N labeled AbetaM01-42 fibrils in vitro from recombinant protein and examined their (13)C-(13)C and (

53 -RNA binding experiments, both in vitro with recombinant protein and in cultured cells with plasmid-e

54 inding sites identified both in the isolated recombinant protein and in virus particles.

55 etic encoding which facilitates their use in recombinant protein and in vivo applications.

56 cause of the lack of high quality functional recombinant protein and insufficient knowledge regarding

57 stinct complexes: one similar in size to the recombinant protein and one much larger.

58 ltransferase, was characterized by assays of recombinant protein and over-expression assays in tobacc

59 Using recombinant proteins and a sensitive protein interaction

60 g the outer layer with fluorescently tagged, recombinant proteins and also tagging the DLP, we distin

61 allenge will allow the synthesis of modified recombinant proteins and augment emerging strategies tha

62 This includes the preparation and testing of recombinant proteins and DNA templates, clustering DNA t

63 es the selection of two Borrelia burgdorferi recombinant proteins and evaluation of their performance

64 ties in production of site-directed mutants, recombinant proteins and exogenous protein overexpressio

65 Additional studies using recombinant proteins and mutant complexes confirmed its

66 Using recombinant proteins and prenylated peptides correspondi

67 hromatography, and immunoblotting using both recombinant proteins and serum/plasma.

68 Using recombinant proteins and transgenic parasites, we show t

69 Candidate antigens were expressed as recombinant proteins and used to analyze the established

70 anine-scanning mutagenesis, loss-of-function recombinant proteins and viruses, and multiple functiona

71 y cytokines can be provided by administering recombinant protein, and intracellular "brakes" of infla

72 mentation analysis, coimmunoprecipitation of recombinant proteins, and bioinformatic analysis reveale

73 ants, by the in vitro enzyme assays with the recombinant proteins, and by the development of Synechoc

74 The recombinant protein anti-EGFR-iRGD also exhibited antitu

75 eraction was analyzed by using purified VWF, recombinant proteins, anti-vimentin antibodies, parallel

76 Insoluble recombinant proteins are a major issue for both structur

77 doids and demonstrate that the corresponding recombinant proteins are also capable of using 8-oxogera

78 doids and demonstrate that the corresponding recombinant proteins are also capable of using 8-oxogera

79 Codon usage plays a crucial role when recombinant proteins are expressed in different organism

80 logenetic studies, transport assays with the recombinant protein as well as GFP-based targeting analy

81 ng HP14 zymogen (proHP14), betaGRP2, and the recombinant proteins as truncated forms showed that the

82 ants and the double-variant (V256I-R525W) as recombinant proteins, as well as a less common variant E

83 n in living cells, but only for a handful of recombinant proteins at a time, whereas the latter can d

84 and -assembly strategy based on a multiphase recombinant protein based material.

85 e a pathway for the development of a simple, recombinant protein-based prophylactic vaccine for HCV w

86 ne (4CMenB) is an outer membrane vesicle and recombinant protein-based vaccine licensed to protect ag

87 These results provide evidence that a recombinant protein-based vaccine targeting the RBDs of

88 aim of assessing the reliability of current recombinant-protein-based data.

89 and behavior of endogenous and/or non-tagged recombinant proteins be analyzed and altered within the

90 y, we explored how an improved DNA prime and recombinant protein boost would impact HIV-specific vacc

91 Total serum binding antibodies against the recombinant proteins (both REG and RMG) were measured by

92 can be reproduced in vitro by incubation of recombinant protein, but the filament growth is very slo

93 ial for not only enhancing the production of recombinant proteins, but also to treat conformational d

94 semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-termina

95 The final method using recombinant protein calibrators and stable isotope label

96 Although monomeric MAVS recombinant protein can assemble into prion-like filamen

97 we report the development of a new class of recombinant protein cancer vaccines that deliver differe

98 t NPQ induction inC. reinhardtii Analysis of recombinant proteins carrying the same mutations refolde

99 Although all recombinant proteins catalyzed the formation of anserine

100 only few options are available for multiple recombinant protein co-expression, and most of them are

101 The 1.07 MDa recombinant protein complex has histone-exchange activit

102 een lacking due to the unavailability of the recombinant protein complex.

103 Membrane-tethered ligands are recombinant proteins comprised of an extracellular pepti

104 s in Escherichia coli and enzyme assays with recombinant proteins confirmed that At5g32470 and its ma

105 Quantitative biophysical studies with recombinant proteins confirmed that H1.0 directly binds

106 D into a cancer therapeutic, we engineered a recombinant protein consisting of the CDD fused to iRGD,

107 We have generated a recombinant protein containing a humanized, dimeric sing

108 onjugating gold nanoparticles (Au NPs) and a recombinant protein containing Renilla luciferase (pRluc

109 MVEC showed that exposure of the cells to a recombinant protein containing the CD36 binding domain o

110 Here we investigate these issues using a recombinant protein corresponding to the N-terminal coil

111 Recombinant proteins corresponding to particular subdoma

112 To address this issue, we generated recombinant proteins covering the entire NC1 domain.

113 Binding studies using recombinant proteins demonstrate that SFPQ and NONO asso

114 Binding assays using purified recombinant proteins demonstrate that the interaction be

115 ace plasmon resonance experiments, 17 mutant recombinant proteins demonstrated a defect in binding to

116 Studies using recombinant proteins demonstrated that SepA is able to c

117 This system leverages the customizability of recombinant protein design to target the conserved recep

118 The recombinant proteins, designated BiFae1A and BiFae1B, we

119 , overexpression of HACE1 by transduction of recombinant protein did not affect proliferation or surv

120 tal infection of mice, but immunization with recombinant protein did not confer protection against ne

121 ed reaction monitoring methods) and in vitro recombinant protein digestion assays.

122 Approaches include peptides, recombinant proteins, DNA, replication-defective viral v

123 yeast has not previously been expressed as a recombinant protein, due to its large molecular mass (25

124 The recombinant protein (Ebp1p-6h) can now be produced by a

125 A recombinant protein encoded by B. burgdorferi BB0723 (a

126 and immunofluorescence staining, as well as recombinant proteins, ERCC Spike-Ins, and population lys

127 NR2, were also expressed in E. coli, and the recombinant proteins exhibited similar kinetic propertie

128 that US9 can direct and control cleavage of recombinant proteins exposed on the luminal leaflet of t

129 fied a minimal core human Ino80 complex from recombinant protein expressed in insect cells.

130 s of endophyte-infected plant tissue and the recombinant protein expressed in the yeast Pichia pastor

131 ncy of pMHC generation from nearly identical recombinant proteins expressed from vaccinia virus and L

132 ive conditions to increase the solubility of recombinant proteins expressed in E. coli.

133 Greater than 30% of recombinant proteins expressed in Escherichia coli (E. c

134 nfirmed direct binding of Scm and PRC2 using recombinant protein expression and colocalization of Scm

135 pathway fluxes as consistent markers of high recombinant protein expression, both in mammalian and mi

136 es, and validated the results by analysis of recombinant protein expression, immunoprecipitation, and

137 noacyl-tRNA depletion due to a high level of recombinant protein expression, ribosome drop-off can su

138 ality was generated by molecular cloning and recombinant protein expression.

139 odon usage is one of the factors influencing recombinant protein expression.

140 ding of malarial actin has instead relied on recombinant protein extracted and purified from heterolo

141 In this report, we characterize the ASN12 recombinant protein for pharmacology in a mouse as well

142 ase activities on C and mC by using purified recombinant proteins for 11 known human APOBEC proteins

143 ly used for the production of biotherapeutic recombinant proteins for a range of molecules including

144 s been widely used to produce many different recombinant proteins for basic research and is being use

145 Results obtained with recombinant proteins for both the hemagglutinin and neur

146 asing demand for biotech-based production of recombinant proteins for use as pharmaceuticals in the f

147 (synthetic peptides, QconCAT constructs, and recombinant proteins) for quantitative accuracy, precisi

148 n, was introduced in a bacterially expressed recombinant protein fragment and biophysically character

149 In addition to the above, using recombinant protein fragments, we were able to localize

150 nic region was defined by Western blot using recombinant protein fragments.

151 iously cloned the taqIIRM gene, purified the recombinant protein from Escherichia coli, and showed th

152 lidated by DNA-binding assays using purified recombinant proteins from the model bacterium Thermotoga

153 We generated recombinant proteins from the mouse cDNAs encoding the H

154 laque, was further investigated by using the recombinant protein generated from E. coli containing a

155 Using recombinant protein H fragments, we narrowed down the bi

156 ated mitochondrial preparations and purified recombinant proteins has confirmed that Sti1p, similar t

157 The recombinant proteins have a C-terminal CVIX protein farn

158 ated mitochondrial preparations and purified recombinant proteins have confirmed that Sti1p is a stro

159 Several recombinant proteins have potential to mediate induction

160 and their expression as biologically active recombinant proteins have provided a basis for studying

161 changes rather than affinity for individual recombinant proteins, have recently attracted renewed in

162 that support high production and quality of recombinant protein in bioprocessing.

163 ELI1 DYW deaminase domain was expressed as a recombinant protein in Escherichia coli and was shown to

164 Expression of the recombinant protein in L. lactis also markedly increased

165 ng production of tyrosine and secretion of a recombinant protein in Saccharomyces cerevisiae by up to

166 tutions in these domains result in a loss of recombinant protein in the culture medium and no associa

167 , viral antigens were introduced as purified recombinant proteins in adjuvant as native proteins.

168 ucted for the rapid and stable expression of recombinant proteins in different plant species, allowin

169 dissociation was analyzed in vitro by using recombinant proteins in ELISA and surface plasmon resona

170 The production of recombinant proteins in Escherichia coli frequently resu

171 st cell extracts as well as when produced as recombinant proteins in Escherichia coli.

172 ProRS isoforms were expressed as His-tagged recombinant proteins in Escherichia coli.

173 een shown to direct high-level production of recombinant proteins in rice suspension cells and germin

174 highly scalable system for the production of recombinant proteins, including virus-like particles (VL

175 Both cvSOD and tcvSOD recombinant proteins inhibited nitroblue tetrazolium red

176 ll-down experiments demonstrated that tagged recombinant proteins interacted to form complexes whose

177 ce optimizations are insufficient to develop recombinant proteins into commercial products.

178 In these vaccines, the recombinant protein is fused with Z12, a novel cell-pene

179 f multiple DNA plasmids, messenger RNAs, and recombinant proteins is developed to allow high spatiote

180 extracellular medium, the coding sequence of recombinant proteins is initially fused to the Saccharom

181 coding retrovirus and in organ culture using recombinant protein, led to intestinal aganglionosis.

182 Microarrays of peptide and recombinant protein libraries are routinely used for hig

183 trong correlation among positive serology to recombinant proteins LinB-13, 26, 15, 21 and to salivary

184 We expressed 91 recombinant proteins, located on the merozoite surface o

185 An EfCP-nanoparticle mimicry made with a recombinant protein, lysenin, revealed its critical cont

186 mal and GAGs-deficient cells showed that the recombinant proteins maintain their GAG-targeting activi

187 ng relevance of plants for the production of recombinant proteins makes understanding the secretory m

188 such a manner that the humoral response to a recombinant protein may be enhanced by coadministering w

189 sorders and for other hepatic diseases where recombinant proteins may be unaffordable or unsuitable.

190 -12, either induced by STAg or injected as a recombinant protein, mediates protection from ECM-associ

191 During the production of recombinant proteins, misincorporation of Nva (norvaline

192 e versatility of this platform in monitoring recombinant protein modification of peptide substrates,

193 y the development of novel functions such as recombinant proteins modified to have different function

194 Recombinant proteins N-glycosylated with S. aureus serot

195 We have constructed a recombinant protein named anti-EGFR-iRGD consisting of a

196 From sucrose alone, the corresponding recombinant protein, named BRS-B, mainly catalyzed sucro

197 Two recombinant proteins, NF2152 and NF2523, were characteri

198 betic mice by treatment with an ecto-TMEM219 recombinant protein normalized circulating IGF-I/IGFBP3

199 tive in quenching in vivo, implying that the recombinant protein obtained is a good material for futu

200 Using recombinant proteins of KIR3DL1*001, KIR3DL1*009, and KI

201 To this end, recombinant proteins of TBV candidates Pfs25, Pfs230, an

202 In sharp contrast, recombinant proteins of the N-terminal region of MUC5B (

203 istration of angiogenic cytokines, either as recombinant protein or as gene therapy, and more recentl

204 It is readily applicable to other recombinant protein or peptide-based imaging probes and

205 (but not to toxoids), whether raised to the recombinant protein or to TcdA26-39 expressed on the B.

206 were validated by enzymatic assays with the recombinant proteins or by their complementation of yeas

207 n producing allergoids or directly producing recombinant proteins or significant peptides, has been e

208 In addition to traditionally used recombinant proteins or synthetic peptides, concatenated

209 mpounds, so-called biologicals (eg, mAbs and recombinant proteins), or by a rather new class of molec

210 rted a Plasmodium yoelii chimeric multistage recombinant protein (P. yoelii linear peptide chimera/re

211 The Arg-degrading recombinant protein, pegylated arginine deiminase (ADI-P

212 f a clickable group at the C terminus of the recombinant protein/peptide with almost complete convers

213 onditions can have on the product quality of recombinant protein pharmaceuticals.

214 (MC-LR) biosensor based on the inhibition of recombinant protein phosphate type 1 (PP1alpha) is repor

215 In experiments with recombinant proteins, phosphorylation of recombinant hum

216 h as examples and showed that these purified recombinant proteins possessed AKR activity using variou

217 n Saccharomyces cerevisiae, we show that the recombinant protein possesses an unusual peroxygenase ac

218 tate the induction of long-term tolerance to recombinant proteins, possibly not only in hemophilia bu

219 ollowing agroinfiltration or infiltration of recombinant proteins produced by Pichia pastoris.

220 onal modifications of hLOXL2 using truncated recombinant proteins produced in Drosophila S2 cells.

221 ber of E. coli expression systems for use in recombinant protein production and represents a major pe

222 workflow should enable future research into recombinant protein production by P. pastoris and a synt

223 Recombinant protein production coopts the host cell mach

224 are a major class of impurities derived from recombinant protein production processes.

225 ysiological and secretory differences across recombinant protein production systems.

226 stabilized cyclopropenones were suitable for recombinant protein production via genetic code expansio

227 visiae is among preferred cell factories for recombinant protein production, and there is increasing

228 ew insights into these cell types, aiding in recombinant protein production, cell growth regulation,

229 th or secretion from nonhuman cells used for recombinant protein production.

230 triazine scaffold enabled its direct use in recombinant protein production.

231 LPs) assemble intracellularly in vivo during recombinant protein production.

232 on yeast, along with biochemical assays with recombinant proteins, provide evidence that Rbins' physi

233 was cloned from Populus trichocarpa, and the recombinant protein (PtDXS) was purified from Escherichi

234 has the ability to produce a wide variety of recombinant proteins, ranging from simple peptides to co

235 Another recombinant protein rDA27(aa 33-84) that removes the GAG

236 Sixteen new recombinant protein reagents were designed to characteri

237 noviral vector and loaded in tandem with the recombinant protein reduced parasite burdens by 76% to >

238 her investigation indicated that most of the recombinant proteins remained in the nuclei of the Sf9 i

239 ion B of the inoculum, while reactivity to a recombinant protein representing the week 8 variant was

240 rus serotype 9 (AAV9) gene therapy vector or recombinant protein, resulted in a complete arrest of fo

241 r magnetic resonance spectroscopy with human recombinant protein revealed that Hsp72 had greater affi

242 inding capacity to approximately 9,400 human recombinant proteins revealed direct binding of TINCR RN

243 rochronic parabiosis or systemic delivery of recombinant protein, reversed functional impairments and

244 of sow and piglet serum samples on a SVA VP1 recombinant protein (rVP1) indirect enzyme-linked immuno

245 ediated conjugation of a small molecule to a recombinant protein scaffold we pave the way to biomedic

246 ghtforward incorporation of Tyr-Leu-Ala into recombinant proteins should make this system attractive

247 Moreover, in vitro kinetic assays with recombinant proteins show that ezrin also is important f

248 Biochemical experiments with purified recombinant proteins show that SSRP1 and Spt16 are able

249 Rac-GEF activity assays with purified recombinant proteins showed that direct interaction with

250 Binding assays with purified recombinant proteins showed that their interaction is di

251 Notably, serum IgG reacted strongly with a recombinant protein spanning MgpB region B of the inocul

252 ent activation products to CC in vitro using recombinant proteins, specific inhibitors, as well as de

253 bent assay) to a range of purified virus and recombinant protein substrates.

254 Central administration of recombinant protein suppressed food intake and altered t

255 A novel recombinant protein tetra-H2A (TH) derived from histone

256 ts requirement for the production of soluble recombinant protein that is functional in processive DNA

257 cell metabolic phenotypes that promote high recombinant protein titer is a major goal of the biotech

258 ia the FLAG epitope, and the ability of this recombinant protein to cleave aggrecan, biglycan, and de

259 Indeed, administration of sFRP1 recombinant protein to the testis in vivo delayed spermi

260 d and screened by phage ELISA using trimeric recombinant proteins to identify viral envelope specific

261 Here we used recombinant proteins to reconstitute and assess lysine a

262 ion for surrogate protein calibrators (i.e., recombinant proteins) to produce inaccurate concentratio

263 r system, pBOMB4, that permits expression of recombinant proteins under constitutive or conditional p

264 Activity assays performed using recombinant protein unveiled differences in substrate bi

265 Additionally, undetected impurities found in recombinant proteins used in cell culture may adversely

266 ponses in a lung injury model, PLTP siRNA or recombinant protein was administered to the lungs of mic

267 Sushi-IL-15-Apo as a recombinant protein was also bioactive in vivo, became c

268 The recombinant protein was easily purified by heat precipit

269 When recombinant protein was expressed in cultured hepatocyte

270 The recombinant protein was expressed, and its kinetic param

271 The recombinant protein was functional when reconstituted in

272 The recombinant protein was hypothesized to be a CBP enzyme

273 The recombinant protein was produced in Escherichia coli and

274 , was expressed in Escherichia coli, and the recombinant protein was purified by nickel-nitrilotriace

275 ed that the mechanical response of the human recombinant protein was remarkably similar to that of th

276 ng in vitro biochemical assays with purified recombinant proteins we characterized four inositol cata

277 Using recombinant protein, we demonstrate for the first time t

278 Using purified recombinant protein, we demonstrated that lymphostatin i

279 Using anti-SLAMF2 Ab and SLAMF4 recombinant protein, we found that SLAMF2 engagement act

280 Using purified recombinant protein, we show that the Leu-Phe substituti

281 a reconstituted system of lipid vesicles and recombinant proteins, we demonstrate that protein-protei

282 Using purified recombinant proteins, we demonstrated that DJ-1 directly

283 By using purified recombinant proteins, we found NLRC3 to interact directl

284 yte and skin resident cell preparations, and recombinant proteins, we have identified that neutrophil

285 Studying this interaction using recombinant proteins, we observed that calcium increases

286 itation and binding studies with natural and recombinant proteins, we show that MRC and CD44 can inte

287 Through the use of the recombinant proteins, we show that the ATP-dependent NNR

288 The allergenic properties of the recombinant protein were tested by ELISA and immunoblott

289 Recombinant proteins were expressed in Escherichia coli

290 Seven recombinant proteins were functionally tested using the

291 cts for wild type and variant allozymes; and recombinant proteins were measured by quantitative Weste

292 Recombinant proteins were shown to have high substrate s

293 essed in a bacterial system and the purified recombinant proteins were used to for antibodies prepara

294 an thrombin, PDGF-BB, Avidin, and His-tagged recombinant protein, were studied, and the results showe

295 macrophages lost the ability to take up the recombinant protein when four highly conserved residues

296 well as M. tuberculosis strains that express recombinant proteins which traffic or fail to traffic to

297 n microscopy; compared the properties of the recombinant protein with those of CLEC3A extracted from

298 tical detection now permits the detection of recombinant proteins with fluorescence excitation at 488

299 ect interaction was confirmed using purified recombinant proteins-with CSB able to modulate the exonu

300 ential role of N-glycosylation in increasing recombinant protein yields in plants.