ライフサイエンスコーパス: recombinantly

1 roperties and are often difficult to produce recombinantly.

2 nt C6, truncated C6 molecules were expressed recombinantly.

3 e detected for the FADY group when expressed recombinantly.

4 X revealed its crucial role in invasion, and recombinantly active PMIX and PMX cleave egress and inva

5 can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-st

6 cence probe of protein structure that can be recombinantly and chemically incorporated into proteins.

7 The 87-amino acid protein was produced recombinantly and demonstrated to bind to fucosylated ol

8 various regions of fibulin-1 were expressed recombinantly and evaluated for their capacity to bind c

9 the first human MACPF domain to be produced recombinantly and in a functional form.

10 a Dengue virus envelope protein was produced recombinantly and tested for its ability to bind antibod

11 preferentially in the testis, was generated recombinantly, and its interactions with cisplatin-modif

12 (7/7 distinct clones/animal) were expressed recombinantly, and shown to recognize the VLPs used for

13 Human QR-2 was prepared recombinantly, and the known QR-2 inhibitor, resveratrol

14 Both proteins, when expressed recombinantly, are active as DNA polymerases and deoxyri

15 G2alpha1 and G2alpha2 domains were prepared recombinantly as the maltose binding protein constructs,

16 Stabilized ECs, expressed recombinantly as VLPs, could be ideal candidate vaccines

17 The engineered domains can be expressed recombinantly at high yield, are predominantly monomeric

18 ealed that mice parenterally vaccinated with recombinantly attenuated staphylococcal enterotoxin (SE)

19 harmacologies that can only be recapitulated recombinantly by a low gamma-7/GluA stoichiometry.

20 A agonists were studied using cAMP assays in recombinantly (CHO) and endogenously (SH-SY5Y) expressin

21 reconstituted this phosphorylation event by recombinantly coexpressing c-Src and Cav-2.

22 he two other missense variants were produced recombinantly: compared to wild type, one (R175P) had no

23 Therefore, bispecific mAbs (bsAbs) were recombinantly constructed from veltuzumab (humanized ant

24 is of the Arabidopsis PhyB isoform assembled recombinantly, coupled with measurements of solution siz

25 Single domain antibodies (sdAbs), which are recombinantly derived from immunized llamas, are known t

26 i; one strain is unambiguously identified as recombinantly derived from other reference strain lineag

27 Two live attenuated, recombinantly derived RSV vaccine candidates, rA2cp248/4

28 The building block was a recombinantly engineered designed tetratricopeptide repe

29 Monomeric mCherry red fluorescent protein recombinantly engineered to express an N-terminal hexahi

30 mode of action of 20b was investigated with recombinantly engineered trypanosomes expressing a cAMP-

31 The scFv antibody isolated was then recombinantly engineered with a human immunoglobulin G1

32 m and structure of the complex, we wanted to recombinantly express IKK in a model organism that lacks

33 We recombinantly expressed 11 different monoclonal antibodi

34 e prohead I architecture (in situ) and for a recombinantly expressed 111-residue peptide (in vitro).

35 plex formed between alpha-bungarotoxin and a recombinantly expressed 19-mer peptide ((178)IPGKRTESFYE

36 involved in Fn binding to alpha IIb beta 3, recombinantly expressed 3fn9--10 module pairs with alani

37 We report that recombinantly expressed 5-HT3 receptor binding sites are

38 We recombinantly expressed a 79-kDa fragment (rPhpA-79) tha

39 The activity of recombinantly expressed ACS6 was recovered in the insolu

40 gion deleted recognized the Escherichia coli recombinantly expressed AL open reading frame on Western

41 In this study, we recombinantly expressed all 36 PvTRAgs using a cell-free

42 Recombinantly expressed alpha- and beta-synucleins inhib

43 Recombinantly expressed alpha1(XI) aminoterminal domain

44 The recombinantly expressed amino-terminal segment of ORF64

45 In tests with recombinantly expressed AMPA receptor subunits, D1 prefe

46 of the phage display-selected inhibitors, we recombinantly expressed and purified 12 SGPI-2 variants

47 Af SidA was recombinantly expressed and purified as a soluble tetram

48 emical consequences of these alterations, we recombinantly expressed and purified both human trypsino

49 or with isoforms of the variable region were recombinantly expressed and purified by affinity and mol

50 Recombinantly expressed and purified gene 2.5 protein-P2

51 In vitro, the recombinantly expressed and purified hADAT1 protein effi

52 Recombinantly expressed and purified MOA retains the spe

53 In reconstitution experiments with recombinantly expressed and purified protein subunits, w

54 y simple free-radical chemistry performed on recombinantly expressed and purified proteins.

55 The dimerization specificity of the recombinantly expressed and purified rod domain of adult

56 The large subunit was recombinantly expressed and purified to near homogeneity

57 three single nucleotide polymorphisms, were recombinantly expressed and purified.

58 Consequently, Gpd2 was recombinantly expressed and purified.

59 erminal globular regions of collagen VI were recombinantly expressed and studied by small angle x-ray

60 Selected B cell Ags were recombinantly expressed and tested in a vaccination prot

61 Finally, we recombinantly expressed and tested the proposed heme deg

62 s rat M1-4 and Nogo-A delta20, proteins were recombinantly expressed and used as substrates for zebra

63 An ALDH1 T244S mutant was recombinantly expressed and was used for mechanistic stu

64 Recombinantly expressed APBP-1 mimics the native cis ele

65 here present the absolute quantification of recombinantly expressed aprotinin out of its host cell p

66 Caveolin was recombinantly expressed as a glutathione S-transferase f

67 Recombinantly expressed as-isolated SOD1 contains a mixt

68 oduct (NifV) from Azotobacter vinelandii was recombinantly expressed at high levels in Escherichia co

69 The Sso7d-mutant hFc binders can be recombinantly expressed at high yield in E. coli and are

70 eported to have tripolyphosphatase activity, recombinantly expressed AtTTM2 unexpectedly exhibited py

71 equence and functional characterization of a recombinantly expressed autoantibody (mAb 131) previousl

72 Recombinantly expressed autoantigens bound specifically

73 Recombinantly expressed AztD purifies with slightly subs

74 The structure of the recombinantly expressed B736L protein was determined by

75 Single domain antibodies are the recombinantly expressed binding fragments derived from h

76 ith fast-protein liquid chromatography using recombinantly expressed C-terminal peptides indicated th

77 ; and (iii) contain approximately 95% of the recombinantly expressed caveolin protein by Western blot

78 We show that recombinantly expressed CCR5 can be incorporated into NA

79 zation of this process is now reported using recombinantly expressed components of SRP from the hyper

80 Recombinantly expressed core protein assembles into T=3

81 Here, we use the recombinantly expressed cytosolic region of VIMP where t

82 nt-negative inhibitors was established using recombinantly expressed defective monomers.

83 Using recombinantly expressed DRADA proteins, we now demonstra

84 Antibodies were prepared against recombinantly expressed EhMIF and used to demonstrate th

85 ignal peptide, as well as for numerous other recombinantly expressed endogenous and heterologous Tat

86 utating arginine 264 to glutamine produced a recombinantly expressed enzyme with nativelike propertie

87 Using recombinantly expressed enzymes and chemically synthesiz

88 Gel filtration of the recombinantly expressed enzymes demonstrated that no cha

89 viously shown to activate PAR-2), we studied recombinantly expressed forms of alpha- and beta-tryptas

90 troscopy, we characterize methylation of (i) recombinantly expressed fragments of Rickettsia typhi Om

91 and were further confirmed with a series of recombinantly expressed fragments.

92 Recombinantly expressed full-length GLB-6 contains a hem

93 The recombinantly expressed fusion protein E437GST, containi

94 escent confocal microscopy demonstrated that recombinantly expressed GABARAP was diffusely localized

95 llent delivery vehicle for the insoluble and recombinantly expressed GLU of GTF and that this constru

96 mportant for the membrane fusion activity of recombinantly expressed GPC.

97 nto other heterotrimer complexes, which when recombinantly expressed had the general single-stranded

98 Measurement of iron in recombinantly expressed HIF-1alpha protein revealed that

99 , we performed a series of experiments using recombinantly expressed homomeric and heteromeric glycin

100 In whole-cell phosphorylation studies, recombinantly expressed human alpha2AAR displayed an inc

101 Recombinantly expressed human ATP:citrate lyase was puri

102 Receptor affinities were determined on recombinantly expressed humGluR6 kainate receptors and o

103 that polyglutamine expansion in purified or recombinantly expressed huntingtin (HTT) proteins affect

104 We demonstrate activation of these recombinantly expressed IKKs by phosphorylation during e

105 ents performed with chimeric beta2-integrins recombinantly expressed in a cell line that is resistant

106 The human sst(4) receptor, recombinantly expressed in Chinese hamster ovary cells,

107 ceptors" (sLRP1-II, sLRP1-III, and sLRP1-IV) recombinantly expressed in CHO cells.

108 tivation of the human somatostatin5 receptor recombinantly expressed in CHO-K1 cells by using some ne

109 yrosine mutants Y93A, D, F, H, S, and W were recombinantly expressed in E. coli and purified to homog

110 However, proteins of interest are often recombinantly expressed in E. coli, where the expression

111 sis thaliana) also produces 3-ketoacids when recombinantly expressed in E. coli.

112 the N-terminus of alpha-bungarotoxin (Bgtx) recombinantly expressed in E. coli.

113 protein-6 (hBMP6) growth factor and could be recombinantly expressed in E. coli.

114 ntly, SOCS2-EloBC and full-length Cul5-Rbx2, recombinantly expressed in Escherichia coli and in Sf21

115 esponding to G179A, G179P, G179T, and G179V, recombinantly expressed in Escherichia coli and purified

116 ion of n-octanoyl-CoA (C(8)-CoA) by WT pMCAD recombinantly expressed in Escherichia coli are similar

117 The gonococcal Fur protein was recombinantly expressed in Escherichia coli HBMV119.

118 These two M. jannaschii genes were recombinantly expressed in Escherichia coli, and their g

119 F63C), SCF (V49L,F63L), and SCF(A165C), were recombinantly expressed in Escherichia coli, purified, a

120 e > 25) was sufficient to suppress LasI/R QS recombinantly expressed in Escherichia coli, whereas onl

121 ase cloned from Flavobacterium heparinum and recombinantly expressed in Escherichia coli.

122 are observed for the binding of mouse HSP47 recombinantly expressed in Escherichia coli.

123 d variants lacking nonenzymatic domains were recombinantly expressed in HEK cell lines.

124 Human TFF2 was recombinantly expressed in HEK-293 cells as a wild type

125 midal neurons or alpha1beta3gamma2 receptors recombinantly expressed in human embryonic kidney 293 ce

126 Full-length human short fibulins were recombinantly expressed in human embryonic kidney cells

127 d Kv1.1 channels (IC50 approximately 15 muM) recombinantly expressed in mammalian cells, induced a po

128 tion and the wild-type and mutated receptors recombinantly expressed in mammalian HEK293T cells were

129 hydrogenase (fdgPDH) from Agaricus meleagris recombinantly expressed in Pichia pastoris .

130 irulence factor of Streptococcus mutans, was recombinantly expressed in the insoluble phase in S. ent

131 renergic alpha1B and muscarinic M3 receptors recombinantly expressed in the same Chinese hamster ovar

132 e glutamate-gated chloride channels (GluCls) recombinantly expressed in Xenopus laevis oocytes with e

133 s to potentiate glutamate-evoked currents at recombinantly expressed KARs.

134 ated, and concatenated polyproteins based on recombinantly expressed maltodextrin-binding protein (MB

135 Here, we have recombinantly expressed mammalian caveolin in Sf21 insec

136 a receptors and can be co-localised with the recombinantly expressed mGluR1alpha/a protein in HEK-293

137 Biochemical characterization of recombinantly expressed mitochondrial LipDH revealed tha

138 Standard detergent solubilization of recombinantly expressed MjK2 required the addition of a

139 Here, using recombinantly expressed molecules and the yeast two-hybr

140 Whereas recombinantly expressed monomers readily aggregated into

141 Recombinantly expressed monomers self-assemble into viru

142 also show that purified mouse brain MBP and recombinantly expressed mouse MBP similarly inhibited Ab

143 Here, we recombinantly expressed murine and human full-length CLE

144 The inhibition against recombinantly expressed mutant forms of aldehyde dehydro

145 ot control samples showed reactivity against recombinantly expressed N-terminal LRP2 fragments on Wes

146 on Western blots and immunoprecipitated the recombinantly expressed N-terminal region of LRP2.

147 Finally, recombinantly expressed NS1 and NS2, individually and to

148 Using recombinantly expressed OPN containing a tag in the N or

149 With recombinantly expressed PDE10A we demonstrate that phosp

150 tions in the subcellular distribution of the recombinantly expressed PKA-RIalpha isoform.

151 Recombinantly expressed PLD2 has high basal activity and

152 immunoblot analysis against each of the nine recombinantly expressed Pmps and quantified by densitome

153 ally relevant form of PR, as crosslinking of recombinantly expressed PR reveals an oligomeric populat

154 1 of the virus polyprotein sequence) and the recombinantly expressed protease domain of NS3.

155 Further biochemical characterization of the recombinantly expressed protein verified it to be a diva

156 evolutionarily conserved AHSP proline 30 in recombinantly expressed proteins and introduced identica

157 protein ligation is a technique for joining recombinantly expressed proteins to polypeptides contain

158 K1 cell line is commonly used for studies of recombinantly expressed proteins, including proteins of

159 employed as a convenient means of purifying recombinantly expressed proteins.

160 nted control over the chemical structures of recombinantly expressed proteins.

161 Recombinantly expressed purified ComB efficiently hydrol

162 rt of reconstitution of an archaeal SRP from recombinantly expressed purified components.

163 Our investigation into recombinantly expressed reflectin has revealed unanticip

164 Properties of recombinantly expressed rIrCD1 are consistent with the e

165 We profiled the binding of recombinantly expressed S1 proteins of TCoV, GfCoV, and

166 Recombinantly expressed shorter Bcl2 family isoforms can

167 t an evaluation of the catalytic activity of recombinantly expressed soluble forms of ADAM19, a prote

168 s work, we have evaluated the utility of two recombinantly expressed Staphylococcal Enterotoxin B (SE

169 e approach to investigating these issues, we recombinantly expressed the N-terminal tail of rat DAT (

170 We recombinantly expressed the two PH variants in Escherich

171 sis, the NaK polypeptide is assembled from a recombinantly expressed thioester peptide and a chemical

172 Recombinantly expressed TrpRS II binds tryptophan (Trp),

173 Six of the recombinantly expressed UGTs conjugated the TNT-transfor

174 We have used NMR spectroscopy on recombinantly expressed uniformly (13)C/(15)N-labeled hu

175 Homomers of human alpha1 GlyR were recombinantly expressed via a baculovirus system, affini

176 Recombinantly expressed virus-like particles (VLPs) coul

177 nstrate that Vpr specifically interacts with recombinantly expressed VprBP in vitro as well as in viv

178 Urea-induced denaturation was studied for recombinantly expressed wild-type betaB1 (WT), the deami

179 reversibly inhibit sodium ion conductance in recombinantly expressed wild-type sodium channels and in

180 Recently, cABCI was cloned, recombinantly expressed, and extensively characterized s

181 missing ARPP phosphatase were found, cloned, recombinantly expressed, and purified.

182 ian, simian, and rodent malaria species were recombinantly expressed, and the proteins were purified

183 Putative SBPs were recombinantly expressed, and their ligand specificity an

184 Recombinantly expressed, purified DmGSTE6 produces predo

185 was identified in Aquifex aeolicus, cloned, recombinantly expressed, purified, and characterized.

186 The chMMP-13 cDNA has been cloned and recombinantly expressed.

187 three aromatic residues (F88/89A-W90A) were recombinantly expressed.

188 preparations of Chinese hamster ovary cells recombinantly expressing the human GPR35.

189 P-1 cells or to stably transfected HEK cells recombinantly expressing their common receptor, CC-CKR-1

190 CD39 was recombinantly fused to an activated GPIIb/IIIa-specific

191 fusion inhibitor (FI) T-651 variant peptides recombinantly fused to the Fc end of a humanized anti-CD

192 -binding Affibody molecule, Z(HER2:342), was recombinantly fused with a C-terminal selenocysteine-con

193 The peptide was recombinantly fused with two high molecular weight (~40k

194 These results suggest that recombinantly fused, complementary, bispecific, anti-CD2

195 We have tested this idea by recombinantly fusing a pH-sensitive green fluorescent pr

196 l envelope glycoprotein gp120--as opposed to recombinantly generated gp120--have not been described.

197 ound potency is determined from assays using recombinantly generated, purified protein.

198 t 214 amino acids in length that is produced recombinantly (GFP 1-10) and a short synthetic peptide c

199 ed form (SmCI N23A mutant) were overproduced recombinantly in a Pichia pastoris system, they displaye

200 uniform virus-like particles when expressed recombinantly in a variety of organisms.

201 ncated C-propeptides of the pro alpha chains recombinantly in bacteria and in a coupled transcription

202 When expressed recombinantly in cells, all altered Lon proteins localiz

203 the human bradykinin B1 receptor, expressed recombinantly in CHO cells.

204 tive cryptococcal mannoprotein was expressed recombinantly in E. coli and Pichia pastoris, resulting

205 The 17 kDa protein was expressed recombinantly in E. coli and purified by affinity chroma

206 expressing the full-length proform of PA-LAP recombinantly in Escherichia coli (here termed, rLAP55)

207 We produced Hm-3 recombinantly in Escherichia coli and determined its str

208 human Notch1 (rLIN-12.1) has been expressed recombinantly in Escherichia coli and purified in a redu

209 isphosphatase from human liver, was produced recombinantly in Escherichia coli and purified in a sing

210 These domains can be produced recombinantly in Escherichia coli at low cost and consta

211 Expression of these genes recombinantly in Escherichia coli resulted in lipid A co

212 croarrays of mouse PDZ domains, all produced recombinantly in Escherichia coli.

213 the corresponding protein has been expressed recombinantly in Escherichia coli.

214 tein, we cloned, sequenced, and expressed it recombinantly in Escherichia coli.

215 rynascus thermophiles (recDHCtCDH) expressed recombinantly in Escherichia coli.

216 assay involving receptor subunits expressed recombinantly in human embryonic kidney cells; the effec

217 ect integrin-binding peptides were expressed recombinantly in Pichia pastoris and were tested for the

218 enzymes restored TAG synthesis when produced recombinantly in yeast H1246 strain.

219 Calkro_0111 was produced recombinantly inEscherichia colias two pieces, containin

220 In this study, recombinantly isolated pANCA monoclonal antibodies were

221 In this study, a recombinantly isolated pANCA monoclonal antibody was use

222 ceuticals, but they are difficult to produce recombinantly, like many other aggregation-prone protein

223 EMP2 expression was recombinantly modified in the ARPE-19 cell line.

224 The ARPE-19 cell line was recombinantly modified to increase the expression of EMP

225 ile self-assembly to QDs, and the ability to recombinantly modify the substrate to target other prote

226 sid protein L1 of papillomaviruses expressed recombinantly or in infected cells has the intrinsic abi

227 with CE-LIF to compare GFP isoforms prepared recombinantly or in vitro with wild-type GFP isoforms is

228 copic data revealed that a large fraction of recombinantly overexpressed AT retains the N-formyl grou

229 d the role of CaBP in these processes by (1) recombinantly overexpressing CaBP in an inducible manner

230 ee gene clusters, DesE, CchF, and CdtB, were recombinantly overproduced in Escherichia coli and purif

231 r isolated during deamidation experiments or recombinantly prepared by site-directed mutagenesis (e.g

232 include the use of mammalian cell systems to recombinantly produce Abs derived from mice bearing huma

233 d an expression system in Pichia pastoris to recombinantly produce and purify Cx43 as well as Cx43 fu

234 To this end, we recombinantly produced and biochemically characterized C

235 Although we were able to show that recombinantly produced and purified protein does not bin

236 present study, skin tests based on ESAT-6, a recombinantly produced antigen highly specific for tuber

237 r detection of residual HCP contamination in recombinantly produced biologics.

238 t failure; one such strategy is the use of a recombinantly produced chimeric TNF alpha soluble recept

239 the complete 14-subunit BoNT/A complex using recombinantly produced components and demonstrated that

240 Recombinantly produced CT1087 and CT0117 proteins had SQ

241 Size-exclusion column chromatography of recombinantly produced Eps15 and of total cell lysates w

242 It was found that adding recombinantly produced fish type III IBPs at a concentra

243 d the efficacy of aflibercept (VEGF Trap), a recombinantly produced fusion protein that scavenges bot

244 in mammalian cells; the first structure of a recombinantly produced G protein-coupled receptor (GPCR)

245 remodeling using commercially available and recombinantly produced glycosyltransferases including ke

246 GPX activity was detected in the recombinantly produced GPX but not with Sj26-glutathione

247 /mass spectrometry were developed to analyze recombinantly produced Group I and Group II human ADP-ri

248 Extending a recombinantly produced HER2 binding affibody molecule wi

249 embly strategy, then subsequently ligated to recombinantly produced histone C-terminal globular domai

250 We show here that recombinantly produced human endostatin interacts with a

251 The protein product of this gene was recombinantly produced in Escherichia coli and purified

252 hermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escherichia coli and shown to

253 white-faced hornets, and Polybia wasps were recombinantly produced in insect cells, immunologically

254 ctionalities in a eukaryotic protein complex recombinantly produced in insect cells.

255 The redox enzyme was recombinantly produced in Pichia pastoris and homogeneou

256 The polypeptide was recombinantly produced in Pichia pastoris, and the three

257 imperfect glycosylation pattern of proteins, recombinantly produced in pro- and eukaryotic hosts.

258 itro xylosylation of a mouse Notch1 fragment recombinantly produced in Sf9 insect cells, a bacteriall

259 First, recombinantly produced kinase was shown to phosphorylate

260 We have recombinantly produced Mac-1 I-domain, and show that it

261 EM to confirm self-assembled structures from recombinantly produced mutants of the naturally occurrin

262 s two alpha(4)beta(1) binding sites within a recombinantly produced N-terminal thrombin fragment of h

263 The effects of recombinantly produced ob protein were compared to those

264 specific and high affinity interaction of a recombinantly produced PIKfyve FYVE domain peptide fragm

265 Residual impurities in recombinantly produced protein biologics, such as host c

266 y of a novel C5 complement inhibitor rEV576, recombinantly produced protein derived from tick saliva,

267 s chemical attachment of aminooxy glycans to recombinantly produced proteins via oxime linkages.

268 Recombinantly produced Px44-green fluorescent protein (G

269 a, while preliminary, clearly shows that the recombinantly produced simplified model system, called B

270 factors, such as Mg2+ or Mn2+, for activity, recombinantly produced SynPPP1 displayed near-maximal ac

271 Upon bacterial aggregation, recombinantly produced TCPs induce permeabilization of E

272 ed and cloned trout IL-2 and have shown that recombinantly produced trout CD80/86 up-regulates the ex

273 We have now cloned, recombinantly produced, and characterized two zebrafish

274 Alfimeprase is a recombinantly produced, genetically modified variant of

275 FXII, derived either from HAEIII patients or recombinantly produced, is defective in mucin-type Thr30

276 model determined using synchrotron data from recombinantly produced, much larger, cryocooled granulov

277 Finally, using recombinantly purified Scc2-Scc4 and cohesin, we perform

278 bes the emerging use of synthetic biology to recombinantly reconstitute plant terpenoid biosynthetic

279 For this purpose, we have recombinantly reconstituted all three subunits together

280 We recombinantly reconstituted the core SHE complex and ass

281 (PTM), we have exploited a eukaryotic PTM to recombinantly synthesize lipid-polypeptide hybrid materi

282 ro folding of mouse alpha- and beta-tubulins recombinantly synthesized in E. coli.

283 When expressed recombinantly these variable domains are termed single d

284 nzymes themselves are slow, complex, and not recombinantly tractable in traditional industrial hosts.

285 ructured oligomerization and can be produced recombinantly using bacterial expression.

286 d in which full-length proteins are produced recombinantly with a protease site inserted between the