ライフサイエンスコーパス: recombinase

1 ine with sebocyte-specific expression of Cre recombinase.

2 e to the recombination activating gene (RAG) recombinase.

3 accessibility of the genomic DNA for the RAG recombinase.

4 as RecA from DNA in its capacity as an anti-recombinase.

5 actions between RSSs, independent of the RAG recombinase.

6 ng (VIP+) GABAergic interneurons express Cre recombinase.

7 is fused to the tamoxifen-inducible CreERT2 recombinase.

8 eflecting properties that are unique to each recombinase.

9 unoglobulin genes be accessible to RAG1-RAG2 recombinase.

10 y eukaryotes have evolved a meiosis-specific recombinase.

11 gulated by the expression of a site-specific recombinase.

12 RecA protein is the prototypical recombinase.

13 ta-isoform of BCCIP in relation to the RAD51 recombinase.

14 ion are activated only after exposure to Cre recombinase.

15 lled by confined injection of adenoviral Cre recombinase.

16 MULE, hAT and Transib elements and the V(D)J recombinase.

17 ical voltage sensor under the control of Cre recombinase.

18 eted to the early DCT using a DCT-driven Cre recombinase.

19 ly, can be conditionally expressed using Cre recombinase.

20 upted in 2 different strains of mice via cre recombinase.

21 activity often associated with helicases and recombinases.

22 ion of an adjacent base triplet by all three recombinases.

23 DSB repair in this bacterium depends on its recombinase A protein (DrRecA).

24 A double-strand break repair proteins RexAB, recombinase A, and polymerase V.

25 of an adeno-associated virus expressing Cre recombinase (AAV-Cre) into the midbrain/pons of mice car

26 deno-associated viral vectors containing Cre recombinase (AAV-Cre).

27 In mice carrying a Flp recombinase-activated allele of Braf (Braf(FA)) in conju

28 aptic circuit, we constructed a panel of Cre recombinase-activated pseudorabies viruses (PRVs) that e

29 ese cells was verified by inducing injury in recombinase-activating gene 2 KO mice, which lack T cell

30 melioration of pathology was observed in the recombinase-activating gene 2 KO mice.

31 The recombinase active site embodies the evolutionary optimi

32 onserved arginine duo (Arg-I; Arg-II) of the recombinase active site plays a crucial role in this fun

33 Two critical steps controlling serine recombinase activity are the remodeling of dimers into t

34 that Y54 phosphorylation enhances the RAD51 recombinase activity by at least two different mechanism

35 we demonstrate that CD45:Cre mouse displays recombinase activity in both myeloid and lymphoid cells.

36 These results indicate that the anti-recombinase activity of BLM is of general importance for

37 ith RAD51, and that BRCA1-BARD1 enhances the recombinase activity of RAD51.

38 Recombinase activity was absent from the central nervous

39 and BRCA1, as well as inhibition of Rad51's recombinase activity, abrogates productive viral replica

40 Accordingly, adenoviral Cre recombinase (AdCre)-treated LSL-Kras/Irs-1(fl/fl) (Kras/

41 own to limit directional tracking of the RAG recombinase along chromatin, and to regulate long-distan

42 O) mouse lines, with viral expression of Cre-recombinase and a light-activated ion channel for optica

43 oach also mediated efficient delivery of Cre recombinase and Cas9:sgRNA complexes into the mouse inne

44 DNA (dsDNA) was then detected directly using recombinase and polymerase proteins through on-chip targ

45 oncogene (KRAS), achieved by delivering Cre recombinase and sgRNAs, which caused rapid lung tumor de

46 d, in that key HR proteins such as the RAD51 recombinase and the tumour suppressors BRCA1 and BRCA2 a

47 However, breeding with a Cre-recombinase and/or Flp-recombinase mouse is required for

48 NA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.

49 esent in analogous positions in other serine recombinases and likely perform similar functions.

50 Although eight yeast site-specific tyrosine recombinases are known, only Flp is actively used in gen

51 ination (HR); assisting the loading of RAD51 recombinase at DNA double-strand breaks.

52 and exchange mechanisms of three RecA-family recombinases, bacterial RecA, and eukaryotic Rad51 and D

53 We developed a recombinase-based framework for building state machines

54 are upregulated in the gut of M. sexta using recombinase-based in vivo expression technology (RIVET).

55 Using a recombinase-based intersectional strategy, these new all

56 rkers (Sox2 and T) and tamoxifen-induced Cre recombinase-based lineage tracing to locate putative NMP

57 ted design of gene regulation programs using recombinase-based state machines.

58 Unexpectedly, we found that R recombinase benefits from the shortening of its C-termin

59 f-SNAPf-Halo2) that are activated by the DNA recombinase Bxb1.

60 triphosphate (ATP) the human RAD51 (HsRAD51) recombinase can form a nucleoprotein filament (NPF) on d

61 We show that these recombinases can operate on DNA sites in mammalian cells

62 the pool of the yeast site-specific tyrosine recombinases capable of mediating genome manipulations i

63 synapsis, providing insight into the serine recombinase catalytic mechanism and how resolvase intera

64 The conserved meiosis-specific Dmc1 recombinase catalyzes homologous recombination triggered

65 Cre recombinase catalyzes the cleavage and religation of DNA

66 The broadly conserved Rad51/RecA family of recombinases catalyzes the DNA strand invasion reaction

67 Such an outcome would be expected if the recombinase complex associates with an IGHJ gene before

68 d by the recombination-activating gene (RAG) recombinase, consisting of RAG-1 and RAG-2 subunits.

69 em recombines DNA sites containing a minimal recombinase core site flanked by guide RNA-specified seq

70 While lineage tracings using cyclization recombinase (Cre) recombinase-mediated cell labeling rep

71 the lung by nasal delivery of adenoviral Cre recombinase (Cre), here we show that KRAS(G12D) expressi

72 In this study, we used the Cre recombinase (Cre)-loxP system under regulation of the mo

73 Sternberg discovered the recombinase, Cre, and its specific locus of crossover, l

74 se-labeled using the tamoxifen-dependent Cre recombinase, CreER(T2), expressed from the endogenous SM

75 (Tff1) gene and the tamoxifen-inducible Cre recombinase (CreERT2)-coding sequence.

76 study, we demonstrate that the site-specific recombinase cyclic recombination (Cre) targeted into the

77 .Foxo1 (L/L) mice with lineage-specific Cre recombinase deletion of floxed FOXO1 and compared the re

78 that exploits GFP for gene manipulation, Cre recombinase dependent on GFP (CRE-DOG), a split componen

79 This strategy relies on CRE recombinase-dependent activation of an EGFP-tagged L10a

80 band of Broca cholinergic neurons using Cre recombinase-dependent adeno-associated virally mediated

81 Optogenetic stimulation using cre recombinase-dependent ChIEF-AAV-DJ expressed in ARC TH n

82 be within a locus designed for efficient Cre recombinase-dependent expression.

83 cotine reward behaviors, we engineered a Cre recombinase-dependent gene expression system to selectiv

84 ne MN-enriched miRNA expression, we used Cre recombinase-dependent miRNA tagging and affinity purific

85 To address this, we used a Cre-recombinase-dependent viral vector approach to express G

86 Strikingly, recombinases derived from unrelated mobile genetic eleme

87 as9 to facilitate use of the dimerisable Cre-recombinase (DiCre) that is frequently used to mediate t

88 ene deletion system based on a dimerised Cre recombinase (diCre) to target CRK3 and elucidate its rol

89 x is a key cofactor for the meiosis-specific recombinase Dmc1.

90 arker into the VTA of male mice that had Cre-recombinase driven by OTR gene expression.

91 ), IGF-1 receptor (IGF1R), or both using Cre-recombinase driven by the adiponectin promoter.

92 eered mouse model in which expression of Cre recombinase driven by the C-type lectin domain family 9,

93 titutive activation of betacatenin using cre recombinase driven by the DEAD (Asp-Glu-Ala-Asp) box pro

94 These include a line for conditional Cre-recombinase-driven inactivation of the gene; a line for

95 These used recombinase drivers targeted different portions of medul

96 d two different mature RPE cell-specific Cre recombinase drivers to inactivate either Dicer1 or DiGeo

97 Using recombinase drivers, we targeted different fractions of

98 rom replication protein A (RPA) to the RAD51 recombinase during DNA break and replication fork repair

99 activation by tamoxifen (TAM)-inducible Cre recombinase Ela-CreERT in the submandibular gland (SMG)

100 Here, through the use of Cre-recombinase-enabled, cell-specific neuron mapping techni

101 mologous DNA recombination (HR) by the RAD51 recombinase enables error-free DNA break repair.

102 s reduced in these strains and controlled by recombinases encoded by the fimE and fimX genes.

103 esistance qacA gene, the cassette chromosome recombinase-encoding genes ccrA and ccrB, and the IS256-

104 3/HDMX complexes in living cells using a new recombinase enhanced bimolecular luciferase complementat

105 ptopatch constructs is controlled by the Cre-recombinase enzyme.

106 ygous T131P mutation in RAD51/FANCR, the key recombinase essential for homologous recombination, in a

107 tes prior to cold exposure, using Pdgfra-Cre recombinase estrogen receptor T2 fusion protein (CreER(T

108 The Rad51 (also known as RecA) family of recombinases executes the critical step in homologous re

109 ouse, and then crossed this mouse with a Cre recombinase expressing mouse driven by the human glial f

110 rons chemogenetically using a retrograde Cre-recombinase-expressing canine adenovirus-2 in combinatio

111 multaneously insert an activity-controllable recombinase-expressing cassette and remove the drug-resi

112 sts with 2 different tamoxifen-inducible Cre recombinase-expressing gene-targeted mouse lines.

113 Using a Cre recombinase-expressing IAV, we have previously shown tha

114 tor fate restriction signals to constitutive recombinase expression enables viral targeting of cell t

115 of floxed KOR or floxed p38alpha MAPK by Cre recombinase expression in dopaminergic neurons blocked p

116 em largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor ce

117 Keratin 14-mediated Cre recombinase expression induced expression of MCPyV T ant

118 ere crossed with DAT-Cre mice, in which Cre- recombinase expression is under dopamine transporter gen

119 osteocytes, no differences in Mbtps1 or cre recombinase expression were observed in cKO SOL, explain

120 After Cre recombinase expression, GsD is activated temporally by t

121 s, and mature keratinocytes through OX40-Cre recombinase expression.

122 DNA recombinases face the daunting task of locating and pair

123 The RAD51 recombinase facilitates DNA joint formation during HR, b

124 Members of the recombinase family can accurately repair double strand b

125 mbination relies on the formation of a Rad51 recombinase filament that forms on single-stranded DNA (

126 single strand annealing proteins (SSAPs) are recombinases frequently encoded in the genome of many ba

127 ions, we used transgenic mice expressing Cre recombinase from the Nkx2.1 promoter to ablate loxP-flan

128 , and a co-dominant-negative effect on RAD51 recombinase function.

129 The RecA recombinase functions to mediate repair via homologous D

130 Notably, TAxI-Cre recombinase fusion proteins induced selective recombinat

131 So far, only one serine recombinase has been analyzed using single substrate mol

132 e recombination activation genes1/2 (Rag1/2) recombinase has evolved from a transposase gene, demonst

133 During T4 homologous recombination, the UvsX recombinase has to compete with the prebound gp32 single

134 Using Cre recombinase, here we show that either deletion or replac

135 ized infusions of adeno-associated virus Cre-recombinase in adult, targeted knock-in mice with loxP s

136 active (Y324F) mutant of this engineered Tre recombinase in complex with the loxLTR DNA substrate.

137 The Ntsr1-Cre GN220 mouse expresses Cre-recombinase in corticothalamic (CT) neurons in neocortic

138 tem, in conjunction with mice expressing Cre recombinase in either parvalbumin-positive, somatostatin

139 gins by stereotactic viral expression of Cre-recombinase in hippocampal CA1 region pyramidal neurons

140 dated a transgenic mouse line expressing cre recombinase in histidine decarboxylase-expressing neuron

141 used to specifically and inducibly drive Cre recombinase in ICC as a strategy to study GIST pathogene

142 Using mice expressing Cre recombinase in MC4R neurons, we demonstrate bidirectiona

143 ice with a knock-in mouse expressing the Cre recombinase in the CD45 locus.

144 We observe a broad expression of Cre recombinase in the Gfi1(Cre) mouse neonatal inner ear, p

145 translated into full-length, functional Cre recombinase in the presence of nonsense suppressors such

146 ession in the NAc of mice transgenic for Cre recombinase in these MSN subtypes.

147 was assessed on interneurons expressing Cre recombinase in vasoactive intestinal peptide (VIP) or pa

148 ted delivery of functional pDNA encoding Cre recombinase in vivo to tissues in transgenic Cre-lox rep

149 xifen-inducible collagen type 2a1-driven Cre recombinase increased proliferation and beta-catenin lev

150 lopment of a transgenic mouse line where Cre-recombinase-induced expression of a mutant methionyl-tRN

151 and contained three predicted site-specific recombinases/integrases and a tetR homologue.

152 o-hybrid method, CrY2H-seq, which uses a Cre recombinase interaction reporter to intracellularly fuse

153 used to deliver the biologically active Cre recombinase into a loxP-reporter T cell line.

154 ntroduction of transgenes encoding the DiCre recombinase into genomic loci dispensable for blood stag

155 lentiviral delivery of shRNAs along with Cre recombinase into lungs of Loxp-stop-Loxp-KRas mice.

156 on of Pbx1 by retroviral transduction of Cre recombinase into Pbx2-deficient SVZ stem and progenitor

157 ximately E11.5) we have therefore used a Cre recombinase introduced at the Ins1 locus.

158 2, the lymphocyte-specific components of the recombinase involved in the process.

159 argeting using the bacteriophage-derived Cre recombinase is widely applied for functional gene studie

160 , unlike the activity of the native R and TD recombinases, is suitable for genome engineering in Esch

161 e activity and the ability to displace Rad51 recombinase, it was unclear which functions were require

162 pithelium of an ErbB2 model coexpressing Cre recombinase led to accelerated tumor onset.

163 y encodes a homologue of an Escherichia coli recombinase, limits biofilm formation in the methicillin

164 However, single gene-driven recombinase lines mark relatively broad and heterogeneou

165 These studies combine the use of the Cre-recombinase/loxP system in mice with optogenetics to str

166 mTERT genes and their neighboring loci, via recombinase-mediated BAC targeting.

167 We applied the Cre/lox recombinase-mediated cassette exchange (RMCE) system to

168 This uses Cre recombinase-mediated cassette exchange to insert a codon

169 MiMIC transposons allow recombinase-mediated cassette exchange to modify the tra

170 ions tested: excision, integration, and dual recombinase-mediated cassette exchange.

171 tracings using cyclization recombinase (Cre) recombinase-mediated cell labeling represent the gold st

172 Here, we used a Cre recombinase-mediated chromosome loss strategy to individ

173 nts in preclinical mouse models requires Cre recombinase-mediated conditional gene expression in stem

174 Using an approach that allows for recombinase-mediated creation or rescue of Nipbl deficie

175 Panx1 (Panx1 (-/-) Apoe (-/-) ) or with Cre recombinase-mediated deletion of Panx1 in endothelial ce

176 a transcriptional driver and by stochastic, recombinase-mediated excision of transcription-terminati

177 ble lineage tracing to fate map, through Cre recombinase-mediated fluorescent reporter gene activatio

178 rosome amplification can be induced by a Cre-recombinase-mediated increase in expression of Polo-like

179 Cre-recombinase-mediated knockdown and overexpression of HDA

180 Cre-recombinase-mediated Ptch1 ablation in mammary epitheliu

181 t we crossed Myh9 floxed mice and Nkx2.5 cre-recombinase mice.

182 be controlled tissue-specifically using Cre recombinase mice.

183 ox-STOP-lox-miR-150 mice with WAP-driven Cre recombinase mice.

184 , breeding with a Cre-recombinase and/or Flp-recombinase mouse is required for the generation of a nu

185 ring HR, members of the RecA/Rad51 family of recombinases must somehow search through vast quantities

186 Cre and Flp recombinase mutants lacking either arginine can be rescu

187 inase (thrombopoietic deletion) or Cd11b-Cre-recombinase (myeloid deletion).

188 distinctive activity of Rad52; neither Rad51 recombinase nor the yeast Rad52 paralog Rad59 has this a

189 nse mutation into the coding sequence of Cre recombinase (nsCre).

190 Upon activation with Cre recombinase ("on-state"), the intron is crippled and the

191 erograde tracing using mice that express Cre recombinase only in neurons producing acetylcholine, glu

192 noparticles with negatively supercharged Cre recombinase or anionic Cas9:single-guide (sg)RNA complex

193 d in research involve knock-in (reporters or recombinases) or gene replacement (e.g., conditional kno

194 of the latter reaction catalyzed by the R/TD recombinase pair shows that the condition supporting the

195 Bacterial Xer site-specific recombinases play an essential genome maintenance role b

196 An isothermal heterogeneous asymmetric recombinase polymerase amplification (haRPA) was carried

197 s under isothermal conditions are presented: recombinase polymerase amplification (RPA) and multiple

198 work describes a proof-of-concept multiplex recombinase polymerase amplification (RPA) assay with la

199 employing isothermal DNA amplification using recombinase polymerase amplification (RPA) for the detec

200 While rapid isothermal strategies such as recombinase polymerase amplification (RPA) have been pro

201 Recombinase polymerase amplification (RPA) is a novel is

202 Recombinase polymerase amplification (RPA) may be used t

203 ion of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method.

204 In a principle study, on-chip recombinase polymerase amplification (RPA) on defined sp

205 uses the isothermal amplification technique recombinase polymerase amplification (RPA) to amplify tr

206 Raman scattering (SERS) labeled nanotags and recombinase polymerase amplification (RPA), which is a r

207 Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was

208 The recombinase polymerase amplification method is modified

209 It can also perform reverse transcription recombinase polymerase amplification reaction (RT-RPA) i

210 eluted and used as a template for isothermal recombinase polymerase amplification, exploiting tailed

211 or cells, either directly through changes in recombinase properties, or indirectly through changes in

212 mbination (HR), which requires the principal recombinase protein Rad51, as well as BRCA1.

213 neered and analyzed variants of two tyrosine recombinases: R and TD.

214 efects in the loading and disassembly of the recombinase RAD-51.

215 nt of inhibitors against the PPI between the recombinase RAD51 and tumour suppressor BRCA2.

216 elieved meiotic inhibition of the ubiquitous recombinase Rad51, suggesting that the mitotic recombina

217 RPA is then replaced by the DNA recombinase Rad51, which forms extended helical filament

218 our suppressor complex, BRCA2-PALB2, and the recombinase RAD51.

219 We wondered if Rad51 recombinase (Rad51), a factor that escorts replication f

220 amage-induced splicing, in which an archaeal recombinase RadA intein splices dramatically faster and

221 The (RAG1-RAG2)2 recombinase (RAG) recognizes recombination signal sequen

222 The RAG1-RAG2 recombinase (RAG1/2) initiates this recombination by cut

223 The RAG recombinase (RAG1/2) plays an essential role in adaptive

224 splicing of the intein in the mycobacterial recombinase RecA is specifically inhibited by the widely

225 nts in which SSB is displaced by the E. coli recombinase RecA.

226 age (e.g. nucleotide excision repair uvrABC, recombinases recBCD and resolvases ruvABC) were not indu

227 pair or insertion of mutations, insertion of recombinase recognition sites, or large DNA elements.

228 of Kras(G12D) (LSL-Kras(G12D)) via Cre(ERTM) recombinase regulated by an acinar cell-specific promote

229 e mice were crossed with mice expressing Cre recombinase, regulated by the villin or CD11c promoters,

230 postnatal testis, and a dual fluorescent Cre recombinase reporter to label FLC and ALC in vivo.

231 knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional a

232 a8 (mcKO) and Zp3 (fcKO) promoter-driven Cre recombinase, respectively.

233 Here, we describe a set of recombinase-responsive fluorescent indicator alleles in

234 sruptive sequence that can be deleted by Cre recombinase, resulting in restored IL-1R1 gene expressio

235 radely transported AAV vector expressing Cre recombinase (Retro-Cre-GFP) into the BLA (Experiment 1)

236 chirality of MeP, in conjunction with mutant recombinases, reveals the stereochemical contributions o

237 eloped a recombinant JHMV that expresses Cre recombinase (rJ-Cre) and infected mice that universally

238 neering include that, whenever possible, the recombinase should act independent of cofactors and that

239 to yeast synthetic chromosome arm synIXR (43 recombinase sites) and then used a computational pipelin

240 diversity through rearrangements at designed recombinase sites.

241 Qi et al. show that recombinases solve this problem by searching in 8-nt mic

242 adenoassociated viral vector expressing Cre recombinase specifically in hepatocytes.

243 Serine and tyrosine site-specific recombinases (SRs and YRs, respectively) provide templat

244 DNA segment exchange by site-specific serine recombinases (SRs) is thought to proceed by rigid-body r

245 However, in these instances, the anti-recombinase, Srs2, is essential to prevent the accumulat

246 Many recombinase strains are in the C57BL/6J background, resu

247 bined with a split-intein-mediated split-Cre-recombinase system in mice to isolate, characterize, and

248 vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable

249 Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the pr

250 potent delivery of nM concentrations of Cre recombinase, TALE- and Cas9-based transcription activato

251 n Cassettes (MiMICs) that contain two phiC31 recombinase target sites and allow the generation of a n

252 lly conserved residues in how they influence recombinase-target site association and formation of 'no

253 Gene-recombinase technologies, such as Cre/loxP-mediated DNA

254 eered mouse models that employ site-specific recombinase technology are important tools for cancer re

255 sarcomas generated with CRISPR-Cas9 and Cre recombinase technology had similar histology, growth kin

256 compare to tumours generated by conventional recombinase technology remains to be fully explored.

257 of 'recCas9', an RNA-programmed small serine recombinase that functions in mammalian cells.

258 RAD51 is a recombinase that maintains replication forks and repairs

259 integrases are virally encoded, specialized recombinases that catalyze the insertion of viral DNA in

260 mouse under the control of both Flp- and Cre-recombinases that is an effective tool for circuit mappi

261 Using two different recombinases, the number of cells labeled and the number

262 o different combinations of Cre, Flp and Dre recombinases, they express eGFP and/or tdTomato to allow

263 ere crossed with mice transgenic for Pf4-Cre-recombinase (thrombopoietic deletion) or Cd11b-Cre-recom

264 f genome stability by interacting with RAD51 recombinase through its C-terminal domain.

265 DNA strand cleavages catalysed by the serine recombinase Tn3 resolvase, we made modified recombinatio

266 mouse model using progesterone receptor-Cre-recombinase to achieve Pten and Grp78 (cPten(f/f)Grp78(f

267 lity was mimicked with viral delivery of Cre recombinase to astrocytes in the LHA and rescued by in v

268 After viral delivery of Cre recombinase to hepatocytes in vivo, GsD is expressed and

269 t it functions in conjunction with the Rad51 recombinase to repair damaged telomeres via the alternat

270 s with a high efficiency of 60% and used Flp recombinase to restore expression in two null cell lines

271 t that phosphorylation of DrRecA enables the recombinase to selectively use abundant dsDNA substrate

272 this protocol does not require heterologous recombinases to insert or excise selective markers from

273 with selective expression of tdTomato or cre recombinase together with optogenetics to investigate wh

274 with a retinoid acid receptor 2 promoter-Cre recombinase transgene (Rarb-cre) expressed in embryonic

275 SMC-specific deletion using SM22-recombinase transgenic allele mice (Runx2(DeltaSM)) led

276 tional knockout [cKO]) when crossed with Cre recombinase transgenic mice.

277 facts associated with beta-cell-specific Cre-recombinase transgenic models, raising questions about t

278 was conditionally deleted in B cells by Cre recombinase under control of the Mb1 gene in Spib (encod

279 ed mice that express tamoxifen-inducible Cre recombinase under control of the Plp1 promoter and carry

280 Adult transgenic mice expressing cre-recombinase under the choline acetyltransferase promoter

281 e with CX3CR1(CreER) mice, which express Cre recombinase under the control of the CX3C chemokine rece

282 ipulated in knock-in mice expressing the Cre recombinase under the endogenous parvalbumin promoter.

283 s floxed at exon 1 to animals expressing Cre recombinase under the pre-proglucagon promoter.

284 ability of BLADE arises from its reliance on recombinases under the control of a single promoter, whi

285 Serine integrases, DNA site-specific recombinases used by bacteriophages for integration and

286 nactive dCas9 to the catalytic domain of Gin recombinase using an optimized fusion architecture.

287 Cre and Flp site-specific recombinase variants harboring point mutations at their

288 The engineered recombinase variants were found to be active in all reco

289 ing an adeno-associated virus serotype-9 Cre recombinase vector (AAV.CBA.Cre).

290 on of a BAAV vector encoding a bacterial Cre recombinase via canalostomy in adult mice with floxed co

291 22-expressing cells, a sequence encoding Cre recombinase was cloned into the Il22 locus, and IL22(Cre

292 cells with adenovirus expressing GFP and Cre-recombinase was successful in GRP78 ablation, and the GF

293 rom cells, Tre, an engineered version of Cre recombinase, was designed to target a 34-bp sequence wit

294 ous recombination in eukaryotes is the RAD51 recombinase, which forms helical nucleoprotein filaments

295 Tyrosine site-specific recombinases, which promote one class of biologically im

296 Here we show that Brujita Int is a simple recombinase, whose properties more closely resemble thos

297 te", here an FF3 fusion, the presence of Flp-recombinase will effectively excise the expression casse

298 of alleles targeted by Cas9 and traditional recombinase with single-cell specificity.

299 fluorescent parasite strains that inject Cre recombinase with their rhoptry proteins (Toxoplasma-Cre)

300 In Escherichia coli, two tyrosine-family recombinases, XerC and XerD, bind to dif and carry out t