ライフサイエンスコーパス: recoverin

1 sented based on the NMR data and homology to recoverin.

2 n, unlike the myristoyl group in Ca(2+)-free recoverin.

3 one bipolars immunoreactive for calbindin or recoverin.

4 ity in Ca(2+) binding, as seen previously in recoverin.

5 overall structure of Frq1 resembles that of recoverin.

6 ct tandem array like that seen previously in recoverin.

7 ring the Ca2+-bound structures of GCAP-2 and recoverin.

8 bipolar cells were labeled with antiserum to recoverin.

9 ylated recoverin to 400 nM for myristoylated recoverin.

10 by Ca2+ and Ca2+-binding proteins, including recoverin.

11 y for phosphodiesterase-gamma, arrestin, and recoverin.

12 myristoyl switch similar to the one found in recoverin.

13 to enter and cause death of cells expressing recoverin.

14 teraction underlies the inhibitory effect of recoverin.

15 fic EF hand Ca2+-binding proteins defined by recoverin.

16 calbindin bipolar cells are also labeled for recoverin.

17 ent inhibition of rhodopsin kinase (GRK1) by recoverin.

18 potency of its effect is similar to that of recoverin.

19 expressing various levels of GRK1 or lacking recoverin.

20 y for binding of Ca(2+) to non-myristoylated recoverin.

21 nd light is nearly eliminated by deletion of recoverin.

22 ness, and bipolar cell types using Chx10 and recoverin.

23 s a sequestered myristoyl group like that of recoverin.

24 immunolabeling with PCNA and either F4/80 or recoverin.

25 od differentiation: rod opsin, arrestin, and recoverin.

26 , F83, and Y86) resembling that of Ca2+-free recoverin.

27 odopsin (Rh*) by Ca(2+)-dependent binding of recoverin, (2) guanylyl cyclase activity via Ca(2+)-depe

28 but prevent the loss of proteins, including recoverin (25 kDa).

29 Recoverin, a 23-kDa Ca(2+)-binding protein of the neuron

30 One such protein is recoverin, a calcium sensor in retinal rod cells, which

31 An antibody to recoverin, a calcium-binding protein found in photorecep

32 Recoverin, a member of the EF-hand protein superfamily,

33 Recoverin, a member of the EF-hand superfamily, serves a

34 Recoverin, a member of the neuronal calcium sensor branc

35 tirecoverin antibodies were purified using a recoverin-affinity column.

36 Interestingly, while the recoverin and calmodulin-binding sites in GRK1 do not ov

37 e highly sequestered myristoyl group seen in recoverin and GCAP1.

38 e feedback on cone phototransduction through recoverin and GRK1 are not well understood.

39 Rods and cones share the same isoforms of recoverin and GRK1, and photoactivation also triggers a

40 In addition, two of the proteins, recoverin and guanylate cyclase activating protein (GCAP

41 , focusing on three Ca(2+)-binding proteins, recoverin and guanylate cyclase activating proteins 1 (G

42 Given the similarities between recoverin and neurocalcin, we examined the effect of Dro

43 genic mutant mice to unravel the function of recoverin and phosducin and to further define the role o

44 Based on their staining with recoverin and salient morphological features, these ecto

45 tibodies against the calcium-binding protein recoverin and the carbohydrate epitope 15 (CD15) as reli

46 Previous experiments genetically deleted recoverin and the GCAPs and showed that significant regu

47 plexiform layer and in immunoreactivity for recoverin and/or CD15.

48 he nerve fiber layer are positive for TULP1, recoverin, and blue cone opsin.

49 inear array, similar to that seen in KChIP1, recoverin, and other structures of the neuronal calcium

50 ns, namely suppression of tumorigenicity 13, recoverin, and Ppib and the fourth binds to human Lactof

51 toreceptor cells, as identified by vimentin, recoverin, and rhodopsin immunocytochemical staining.

52 yclase activating protein, protein kinase A, recoverin, and transducin) are N-terminally modified wit

53 ng carcinoma: retinopathy ("CAR"-IgG [23kDa, recoverin]) and optic neuritis collapsin response-mediat

54 e ratio of rhodopsin kinase to its modulator recoverin appears critical for the proper adaptation of

55 Recoverin appears in cones across 70% of the retina at f

56 e studies showed that antibodies specific to recoverin are able to enter and cause death of cells exp

57 The first eight residues of recoverin at the N terminus are solvent-exposed, enablin

58 of (15)N-labeled Ca(2+)-bound myristoylated recoverin bind anisotropically to phospholipid membranes

59 eport here the NMR structure of Ca(2+)-bound recoverin bound to a functional N-terminal fragment of r

60 Calsenilin/DREAM/KChIP3, a member of the recoverin branch of the EF-hand superfamily, interacts w

61 At high Ca2+ (Ca), myristoylated recoverin (Ca-recoverin) prolonged the recovery phase of

62 )H NMR order parameter analysis performed on recoverin containing a fully deuterated myristoyl group,

63 nglion cells were shortened when measured in recoverin-deficient retinas.

64 EF-1, as in recoverin, does not bind calcium because it contains a d

65 Instead, it is an effect of recoverin downstream of phototransduction in rods that p

66 , autoantibodies originally elicited against recoverin expressed in tumor cells may damage retinal ph

67 oup covalently attached to the N-terminus of recoverin facilitates its binding to retinal disk membra

68 oup covalently attached to the N-terminus of recoverin facilitates the binding of recoverin to retina

69 f GCAP-2, like those of other members of the recoverin family of Ca2+-binding proteins, is fatty acyl

70 calcium-dependent manner, reminiscent of the recoverin family of calcium-myristoyl switch proteins.

71 m-binding motif) that is most similar to the recoverin family of myristoyl switch proteins.

72 main that is related to the N termini of the recoverin family of neuronal calcium sensors.

73 Calsenilin is a member of the recoverin family of neuronal calcium-binding proteins th

74 labeled recoverin to membranes and show that recoverin favors membranes with negative curvature and h

75 ylation had little effect on the affinity of recoverin for the kinase, but it raised the K0.5 for Ca2

76 In the present work, the affinity of recoverin for the negatively charged phosphatidylserine

77 Frq1 belongs to the recoverin/frequenin branch of the EF-hand superfamily an

78 of four Ca(2+)-sensor proteins (calmodulin, recoverin, GCAP1, and GCAP2) operating in the vertebrate

79 hors found no evidence that mutations in the recoverin gene are a cause of RP or another of the hered

80 n phenotype associated with mutations of the recoverin gene remains unknown.

81 odulin-binding sites in GRK1 do not overlap, recoverin-GRK1 interaction is inhibited by calmodulin, m

82 unique N-terminally localized GRK1 site for recoverin had no clearly defined structural characterist

83 Recoverin has a relative molecular mass of 23,000 (M[r]

84 -/-) rods, in which the GRK1-binding protein recoverin has been genetically deleted.

85 Recoverin has been identified as a target autoantigen fo

86 Recoverin has been postulated to inhibit the kinase in d

87 Recoverin has two functional EF hands and a myristoylate

88 One such protein, recoverin, has been proposed to regulate RK activity con

89 he myristoyl binding site and two swivels in recoverin homologues from yeast to humans indicates that

90 It also contains an N-terminal recoverin homology (RVH) domain that is related to the N

91 cell (protein kinase C-alpha [PKC-alpha] and recoverin) immunofluorescence revealed the maintenance o

92 it may reduce the inhibitory constraint that recoverin imposes on rhodopsin kinase, an enzyme respons

93 icular lipids and of specific amino acids of recoverin in its membrane binding has not yet been demon

94 serine, whereas the extent of penetration of recoverin in phosphatidylserine monolayers was estimated

95 that light causes a significant reduction of recoverin in rod outer segments, accompanied by its redi

96 GCAP-2 differs from recoverin in that the calcium ion binds to EF-4 in addit

97 lex is similar to structures of Ca(2+)-bound recoverin in the absence of target (<1.8A root-mean-squa

98 The overall main-chain structure of recoverin in the complex is similar to structures of Ca(

99 origin and identity of the cells expressing recoverin in the ganglion cell layer of the rat retina.

100 ed for the membrane binding of myristoylated recoverin in the presence of calcium.

101 oantibody to recoverin, when given access to recoverin in the retina through the blood-retina barrier

102 periments indicate that rhodopsin kinase and recoverin, in addition to their well-known role in regul

103 The myristoyl (C14:0) modification of recoverin increased its activity 12-fold, and the C12:0

104 gy model of CIB based upon calcineurin B and recoverin indicated a conserved hydrophobic pocket withi

105 It has been shown that recoverin inhibits the phosphorylation of rhodopsin when

106 Recoverin is a 23 kDa myristoylated Ca2+-binding protein

107 Recoverin is a heterogeneously acylated calcium-binding

108 Recoverin is a protein in rods that prolongs phototransd

109 Recoverin is a small calcium binding protein involved in

110 results demonstrate that membrane binding by recoverin is achieved primarily by insertion of the myri

111 We propose that Ca(2+)-bound recoverin is bound between rhodopsin and RK in a ternary

112 studies revealed that the myristoyl group of recoverin is sequestered inside the protein core in the

113 The Ca(2+)-binding protein recoverin is thought to regulate rhodopsin kinase and to

114 this no longer occurs in mice that have had recoverin knocked out.

115 The binding of two Ca2+ ions to recoverin leads to its translocation from the cytosol to

116 second and third EF-hands (EF-2 and EF-3) of recoverin leads to the extrusion of the fatty acid.

117 l cyclase-activating protein 2 (GCAP-2) is a recoverin-like calcium-binding protein that regulates ph

118 esponding fragments of other closely related recoverin-like proteins that do not regulate RetGC.

119 s to be an assayable property of a subset of recoverin-like proteins.

120 exposed hydrophobic groove on the surface of recoverin lined by side-chain atoms of Trp-31, Phe-35, P

121 tivation and that the Ca(2+)-binding protein recoverin may be required for the light-dependent modula

122 These experiments support the notion that recoverin mediates Ca-dependent inhibition of rhodopsin

123 d calcium-binding studies of a myristoylated recoverin mutant (myr-E85Q) designed to abolish high-aff

124 calcium-binding proteins closely related to recoverin, neurocalcin, and many other neuronal Ca(2+)-s

125 neuronal calcium sensor (NCS) proteins (e.g. recoverin, neurocalcins, and frequenin) are expressed at

126 sor protein-1 (NCS-1), another member of the recoverin-neuronal calcium sensor superfamily, is expres

127 Unexpectedly, the effect of recoverin on postsynaptic signals could not be explained

128 Transplanted cells coexpressed GFP and recoverin only in the ONL.

129 ng N-terminal region can be substituted with recoverin or neurocalcin sequences without loss of GCAP-

130 corresponding fragments from neurocalcin or recoverin, or even partially deleted without preventing

131 Dye injections of recoverin- or CD15-prelabeled cone bipolar cells in vert

132 photoreceptor-specific Ca2+-binding protein recoverin, other GRKs can be inhibited by Ca2+-calmoduli

133 inal mixed cultures, MCP-1 was cytotoxic for recoverin+ photoreceptors, and this toxicity was elimina

134 The N-terminal region of recoverin points toward the membrane surface, with close

135 strate that early in development some of the recoverin-positive cells in the ganglion cell layer are

136 velopment, there were also a small number of recoverin-positive cells of unknown origin in the gangli

137 Recoverin-positive cells were apparent in the ventricula

138 These recoverin-positive cells were not double-labeled by cell

139 Another contingent of recoverin-positive cells, with morphological features eq

140 ologies of photoreceptors, horizontal cells, recoverin-positive OFF-cone bipolar cells, rod bipolar c

141 cells is not required for the segregation of recoverin-positive On and Off cone bipolar cell projecti

142 d biotinylated antibodies were cultured with recoverin-positive rat retinal cells E1A.NR3.

143 the calcium-binding proteins calmodulin and recoverin, posttranslational isoprenylation and palmitoy

144 We also find that recoverin potentiates the sensitivity of cones in dim li

145 high Ca2+ (Ca), myristoylated recoverin (Ca-recoverin) prolonged the recovery phase of the bright fl

146 pigment epithelium-derived factor (PEDF) and recoverin (RCV1) map to this region and are candidate ge

147 These cells expressed photoreceptor proteins recoverin, red opsin, and rhodopsin, and displayed morph

148 Indeed, calcium binding by recoverin results in the extrusion of its myristoyl grou

149 ra of recoverin suggests that membrane-bound recoverin retains the same overall three-dimensional str

150 luding presumptive photoreceptors expressing recoverin, rhodopsin, or cone opsin.

151 ported effects of adenine nucleotides on the recoverin-rhodopsin kinase binding.

152 ristoylation, and adenine nucleotides on the recoverin-rhodopsin kinase interaction.

153 in when Ca2+ is present and that a dependent recoverin/rhodopsin kinase interaction underlies the inh

154 -B1/B2, GAD67, GLRA1b, GRM1, GRM5, Hu, LGl1, recoverin, Ri, ZIC4).

155 The recoverin-RK25 complex exhibits Ca(2+)-induced binding t

156 a of uniformly and selectively (15)N-labeled recoverin show that the Ca(2+)-bound protein is position

157 observed throughout the culture treated with recoverin specific antibodies but not with normal antibo

158 al myristoylation, similar to members of the recoverin subfamily and are fatty acid acylated in vitro

159 em array similar to GCAP2 and members of the recoverin subfamily of Ca2+-binding proteins.

160 t GCIP is a Ca2+-binding protein of the GCAP/recoverin subfamily.

161 1 were very similar to those of Ca(2+)-bound recoverin, suggesting that the overall structure of Frq1

162 nce of ncs1Delta was not affected by cAMP or recoverin, suggesting that the two ncs1Delta phenotypes

163 al and calculated solid-state NMR spectra of recoverin suggests that membrane-bound recoverin retains

164 osed drop in Ca concentration, the effect of recoverin switched off with little lag.

165 Residues of recoverin that contact RK25 are highly conserved, sugges

166 ls were visualized using an antibody against recoverin, the calcium binding protein that labels On an

167 ls were visualized using an antibody against recoverin, the calcium-binding protein that labels the s

168 An antibody against recoverin, the calcium-binding protein, labels photorece

169 he K0.5 for Ca2+ from 150 nM for nonacylated recoverin to 400 nM for myristoylated recoverin.

170 analyses confirmed that Ca2+ is required for recoverin to bind RK.

171 e Ca(2+)-responsive translocation of labeled recoverin to membranes and show that recoverin favors me

172 ion of the specific binding of myristoylated recoverin to phosphatidylserine, whereas the extent of p

173 inus of recoverin facilitates the binding of recoverin to retinal disk membranes by a mechanism known

174 We suggest that recoverin translocation is adaptive because it may reduc

175 Recoverin undergoes a calcium-myristoyl switch during vi

176 In both cases the majority of recoverin was found in rod inner segments, with approxim

177 Moreover, the calcium-myristoyl switch of recoverin was only observed upon binding onto monolayers

178 Results suggest that autoantibody to recoverin, when given access to recoverin in the retina

179 sidues 2-90) is similar to that of Ca2+-free recoverin, whereas the C-terminal region (residues 100-2

180 f ncs1Delta was also complemented by retinal recoverin, which controls Ca2+-regulated desensitization

181 cule or protein the myristoyl-switch protein recoverin, which is involved in rhodopsin-mediated signa

182 epresents a hybrid between the structures of recoverin with zero and two Ca2+ bound.

183 Cells not expressing recoverin (Y79, PC12, and GH3) were not susceptible to c