ライフサイエンスコーパス: refold

1 group I intron structures, allowing them to refold.

2 first and then transferred to DnaK2 where it refolded.

3 it C-terminus additionally assist in subunit refolding.

4 cient time for protein chaperones to attempt refolding.

5 agglutinin started to undergo conformational refolding.

6 1, to partner with Hsp70 in in vitro protein refolding.

7 transferred to ATP-dependent chaperones for refolding.

8 d using limited proteolysis and denaturation/refolding.

9 gy transfer in the microsecond time range of refolding.

10 refolding, and, subsequently, again tertiary refolding.

11 everal times faster than that of spontaneous refolding.

12 ve regions of a protein chain and preventing refolding.

13 e mechanism of chaperone-assisted Luciferase refolding.

14 ve helical structure frustrating microsecond refolding.

15 te structural dynamics with global unfolding/refolding.

16 ammaD-crystallin aggregation suppression and refolding.

17 trap as the reason for slower P-jump-induced refolding.

18 vitro assay for this step in fusion protein refolding.

19 an anticipated from models involving protein refolding.

20 from one another, leading to native protein refolding.

21 rin cytoskeleton, including domain unfolding/refolding.

22 onformational changes that trigger F protein refolding.

23 and ligand binding-dependent conformational refolding.

24 compact and extended states populated during refolding.

25 e of events could elucidate the mechanism of refolding.

26 ffinity purification and solid-phase protein refolding.

27 s to undergo ligand-dependent conformational refolding.

28 s that H223 protonation guards against early refolding.

29 protein aggregates, leading to their native refolding.

30 repeated rounds of stepwise G4-unfolding and refolding.

31 d) and the P3-strengthening mutations slowed refolding (6- to 1400-fold), suggesting that P3 indeed u

32 photochemical deprotection is sufficient to refold a helical oligomer, interconverting M and P screw

33 cific subunits with negatively charged tags, refolded a mixture of monomers, and used ion-exchange ch

34 one mutation abolished both Hsc70 ATPase and refolding activities.

35 a similar structure, ATP use, and substrate refolding activity, and, importantly, it also inhibits m

36 less than temperature denaturation, protein refolding after a fast P-jump is not necessarily faster

37 Upon refolding after chemical denaturation, this protein prod

38 vinculin and vinculin binding inhibits talin refolding after force is released.

39 unfolding temperature but rather facilitated refolding after thermal stress.

40 plexes with heat shock protein 90 (HSP90) to refold aggregated protein substrates.

41 However, in lieu of fostering their refolding, Aha1 allows ubiquitination of bound clients b

42 of CFA/I fimbriae, is able to spontaneously refold and polymerize into cyclic trimers.

43 two structurally different phycobiliproteins refold and reassemble in a cooperative manner from their

44 aggregation and inactivation and was able to refold and restore their enzymatic activities after heat

45 d ability of disulfide-bonded RRM2 dimers to refold and their increased propensity to misfold, which

46 ss the blood brain barrier help mutant GCase refold and traffic correctly to lysosomes are putative t

47 nins up to 80 degrees C, followed by partial refolding and aggregation at even higher temperatures.

48 ich, marginally stable oligomers in vitro on refolding and cross-beta-rich aggregates following incub

49 Allosteric effectors that mediate refolding and enhance enzymatic function have the potent

50 RiC and were active as assayed by luciferase refolding and human gammaD-crystallin aggregation suppre

51 apable of growing on the ends by seeding the refolding and incorporation of the normal form of the gi

52 Both experimental refolding and Monte Carlo simulations of Markov state mo

53 ssary early step for paramyxovirus F-protein refolding and presents a novel target for structure-base

54 ess conditions, when both chaperone-mediated refolding and proteasomal degradation are compromised or

55 on in Escherichia coli followed by oxidative refolding and proteolysis.

56 activated beta-cardiac myosin is followed by refolding and reactivation of ATPase and motile activiti

57 is, including quality control during protein refolding and regulation of protein degradation.

58 hibited complex dynamics, including frequent refolding and state occupancies of <10 mus.

59 tigens, this antigen preparation induces MR1 refolding and upregulates surface expression of human MR

60 ctive site was produced in Escherichia coli, refolded, and processed in vitro into a mature enzyme.

61 h display tertiary unfolding, then secondary refolding, and, subsequently, again tertiary refolding.

62 arance pathways involved in the recognition, refolding, and/or clearance of aberrant proteins.

63 ed using typical dialysis- or dilution-based refolding approaches.

64 ionally folding protein whose extremely slow refolding (approximately days) is catalyzed by chaperone

65 ks that can be seen to repeatedly unfold and refold as units even under native conditions.

66 In vitro refolding assays under redox conditions showed that POFU

67 of the combinations in ATPase and luciferase refolding assays were dependent on the identity and stoi

68 phase, but also of a ~1.5 ms "slow" phase of refolding, attributed to non-native helical structure fr

69 Denaturation and refolding behavior of the allergen confirmed that its Ig

70 cium, the R1597W mutation slowed the rate of refolding but had no effect on unfolding.

71 -denatured malate dehydrogenase that did not refold by the assistance of the DnaK2 chaperone system a

72 mbrane proteins denatured in SDS can also be refolded by addition of NIS.

73 found that CyaA forms mainly multimers when refolded by dialysis, dilution, or buffer exchange.

74 heir complexes with SDS were attempted to be refolded by the addition of C12E8, while DDM was additio

75 amino acid foldon at the C terminus and slow refolding channeled gp41 into trimers.

76 by refolding is challenging because suitable refolding conditions must be empirically determined for

77 based determinations with respect to varying refolding conditions.

78 slational modifications, they must either be refolded correctly or recycled.

79 nd fold rapidly with overlapping melting and refolding curves, G3T multimers (G3T units covalently at

80 Refolded D-Wt molecules form oligomers with high sedimen

81 increases the expression of chaperones that refold damaged misfolded proteins or facilitate their de

82 hypothesized that much like disaggregation, refolding, degradation, and even normal function, Hsc70

83 oordinated the ability of Hsp22 and Hsp70 to refold denatured luciferase in vitro.

84 e resulting method, which we deem DSF guided refolding (DGR), thus enables the production of aggregat

85 Attempts to refold DsbB and GlpG from chemically denatured states in

86 cessible means to characterize the unfolding/refolding dynamics of individual molecules and resolve c

87 ifferent kinetic constants for unfolding and refolding even though the process remains experimentally

88 (H or G) triggers F to undergo an extensive refolding event to form a stable postfusion state.

89 insights into the mechanics of this critical refolding event.

90 Recent P-jump refolding experiments on the helix bundle lambda-repress

91 Single-molecule refolding experiments reveal the initial nucleation of f

92 In this study, we examine the mechanism of refolding for two distinct rhomboids to gain insight int

93 ces the difference between the unfolding and refolding forces, bringing the non-equilibrium unfolding

94 In vitro refolded forms of tapasin-independent allotypes assemble

95 oprotein obtained following denaturation and refolding forms a hexamer.

96 by the viral fusogen gB, which is thought to refold from the prefusion to the postfusion form in a se

97 in protein-free RNA but others would need to refold from their dominant solution conformations to for

98 ational modifications and usually need to be refolded from inclusion bodies.

99 e that was expressed in Escherichia coli and refolded from inclusion bodies.

100 n-prone and disulfide-containing proteins by refolding from E. coli inclusion bodies, which would not

101 As F undergoes a dramatic refolding from its prefusion to postfusion conformation,

102 The P3-weakening mutations accelerated refolding from M (3- to 30-fold) and the P3-strengthenin

103 olding of native peripheral structure during refolding from M, which probably permits rearrangement o

104 cid-induced fusion, as well as insights into refolding from pre- to post-fusion conformations.

105 e helix-turn interfaces that should speed up refolding from the pressure-denatured state, if this hyp

106 al reports the crystallographic structure of refolded, full-length SMN protein and two disease-releva

107 e that FtsH contains the protease as well as refolding functions, and both the AAA and the proteolyti

108 Mammalian prions refold host glycosylphosphatidylinositol-anchored PrP(C)

109 Thus, pressure-jump (P-jump)-induced protein refolding, if it could be made fast enough, would be ide

110 al surface glycoproteins, unleashing them to refold in ways that catalyze virus-cell membrane fusions

111 Biophysical analysis of constructs refolded in vitro demonstrated that MOSP(C), previously

112 Mini-H2-L(d) refolded in vitro with a high affinity peptide yields a

113 mutants were expressed in Escherichia coli, refolded in vitro, and used to immunize rabbits.

114 in in vivo, versus the structure formed when refolded in vitro.

115 a-repressor mutant is nonetheless capable of refolding in a single explicit solvent MD trajectory in

116 as measured by the inhibition of luciferase refolding in prostate cancer cells.

117 ev1) disrupts G4 DNA structures and prevents refolding in vitro.

118 ve the kinetics of spontaneous unfolding and refolding in zero urea.

119 variant-TCTP11-31 complex reveals that TCTP refolds in a helical conformation upon binding the BH3-g

120 the Mg(2+)-bound state of NCS-1 unfolds and refolds in a three-state process by populating one inter

121 Here we report a rapid method for refolding inclusion-body-based, recombinant cell surface

122 At an interface, these cap residues refold, inserting the hydrophobic side chains into the a

123 We probed the formation of a refolding intermediate by time-resolved fluorescence ene

124 ibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies.

125 Upon relaxation, the unfolded rubredoxin can refold into its native holo state with the reconstituted

126 ampled sequences versus those sequences that refold into the same structure and derive a criterion to

127 nant virions efficiently and were capable of refolding into a postfusion conformation without tempora

128 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

129 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

130 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

131 usogenic glycoproteins of these pathogens is refolding into a thermodynamically highly stable fusion

132 he unfolded protein prevents rubredoxin from refolding into its native holo state.

133 ediate structures of the MACPF domain during refolding into the beta-barrel pore establish a structur

134 mers from the aggregate and their subsequent refolding into the native conformation.

135 ngly compact, and the heme coordination loop refolds into a beta-hairpin with a turn formed by the hi

136 tion wherein the domains dissociate and RfaH refolds into a NusG-like structure.

137 se to facilitate transcription while the CTD refolds into the beta-barrel conformation that interacts

138 ss clear how the unbound, sensing riboswitch refolds into the ligand binding-induced output state.

139 s to a lower volume monomeric PrP state that refolds into the native conformation after pressure rele

140 NMR analyses to show that the denatured RfaH refolds into the native state and that RfaH in which the

141 Domain 3 of the soluble toxin refolds into two 85 A beta-hairpins that traverse the l

142 ReFOLD is a novel hybrid refinement server with integrat

143 the non-native client proteins bound to HtpG refold is of central importance to comprehend the essent

144 In practice, however, protein production by refolding is challenging because suitable refolding cond

145 Loop refolding is limited by the hydrophobic collapse of the

146 NTE as influencing the chaperone-independent refolding kinetics and overall thermodynamic stability o

147 we report the force-dependent unfolding and refolding kinetics of all talin rod domains.

148 of microsecond pressure and temperature jump refolding kinetics of the engineered WW domain FiP35, a

149 cond pressure-jump apparatus, we monitor the refolding kinetics of the helix-stabilized five-helix bu

150 of folding against force and accelerates the refolding kinetics.

151 n-random client proteins of the Hsp104/Hsp70-refolding machinery, including the prion Sup35.

152 omes bind to a mRNA faster than the mRNA can refold, maintaining it in a nonequilibrium state with an

153 partners and that context-dependent protein refolding may be widespread in nature.

154 ts demonstrate a cooperative, self-chaperone refolding mechanism, whereby the beta-subunits independe

155 Thus, activation or restoration of refolding mechanisms may alleviate TDP-43 aggregation in

156 To investigate the dynamic nature of MHC, we refolded MHC proteins with peptides that can be hydrolyz

157 BiP's ATPase activity, which is required for refolding misfolded proteins while coping with ER stress

158 oduces a kinetic barrier that partitions the refolding molecules to the nonpermuted structure.

159 ation is inhibited and with that of in vitro refolded mRNA.

160 our results suggest that initial Luciferase refolding occurs along a vectorial pathway and also sugg

161 nometre-scale rearrangement is controlled by refolding of an evolutionarily conserved 'tongue', which

162 f the SDS from the protein-SDS complexes and refolding of betaLG, BSA, and lysozyme, while alphaLA ch

163 Refolding of both proteins results in reassociation of t

164 f HMLalpha as the donor, when we perturb the refolding of chromosome III.

165 of Hsp90 interacts with disordered CTA1, and refolding of CTA1 by Hsp90 is dependent upon ATP hydroly

166 es a process that couples the Hsp90-mediated refolding of CTA1 with CTA1 extraction from the ER.

167 mc and also inhibits Cosmc-assisted in vitro refolding of denatured T-synthase.

168 Yet ARF6 alone did not promote the refolding of disordered CTA1 to an active state.

169 tained from stopped-flow measurements of the refolding of Escherichia coli adenylate kinase were anal

170 other DNA polymerases, or hRev1 can prevent refolding of G4 DNA structures.

171 the activation energy required to cause the refolding of gB from a prefusion to a postfusion conform

172 ion pattern of gB, possibly representing the refolding of gB from its prefusion to its postfusion con

173 virus (EBV) is thought to be mediated by the refolding of glycoprotein B (gB) from a prefusion to a p

174 ical energy through mechanical unfolding and refolding of isopeptide bond-delimited polypeptide loops

175 ging to observe the reversible unfolding and refolding of metalloproteins because of a loss or decomp

176 to chaperones, which aid in the clearance or refolding of misfolded proteins.

177 e native folding of nascent polypeptides and refolding of misfolded species, thereby buffering mutati

178 At slow pulling speeds (<50 nm s(-1)), the refolding of NuG2 can be clearly observed.

179 biquitous chaperone is to participate in the refolding of proteins denatured by cytoplasmic stress, t

180 pid cofactors may facilitate the spontaneous refolding of PrP into an infectious form while also allo

181 rences between protein folding in the ER and refolding of purified proteins.

182 pe III secretion, although contribution from refolding of secreted proteins has not been ruled out.

183 ozymes could be restored by putative t2M/t4M refolding of stem secondary structure or tertiary bridgi

184 hat force-dependent stochastic unfolding and refolding of talin rod domains make talin a very effecti

185 Surprisingly, we find that during refolding of tandem repeats, independent of sequence ide

186 Defective refolding of TDP-43 is predicted to aggravate the TDP-43

187 rotein and RNA components requires extensive refolding of the 16S rRNA and is assisted by 10-20 assem

188 ring, which enables reversible unfolding and refolding of the chains.

189 tute for DnaJ2 or GrpE in the DnaK2-assisted refolding of the denatured substrates.

190 Upon relaxation, refolding of the ferredoxin-like domains enables the hyd

191 diated GQ unfolding is typically followed by refolding of the GQ, a pattern that is repeated several

192 dependent conformational destabilization and refolding of the hole-hole homodimer Fc.

193 Here, the reversible unfolding-refolding of the iron-sulfur protein rubredoxin was obse

194 terized the molecular events associated with refolding of the metastable prefusion S glycoprotein to

195 Functional rescue was observed on refolding of the oligo-T/U strands into bPNA triplex hyb

196 er virus, we show that these changes involve refolding of the protein into a trimeric state.

197 and peptides suggests that SER5 also delays refolding of the remaining fusion-competent Env trimers.

198 This bridging action requires a complete refolding of the RfaH C-terminal domain (CTD) from an al

199 observed in the p53-MDM2 complex and induces refolding of the short, unstructured MDM2 N-terminal reg

200 otif affects only late intermediate R3, when refolding of the tongue and docking to the GAF1 domain a

201 Refolding of the U-loops into bPNA triplex stems complet

202 ified based on changes in the elasticity and refolding of the unfolded polyprotein in the presence of

203 pendent activation, titin elastic recoil and refolding of titin domains as an energy source, and Ca(2

204 e MACPF domain, accompanied by extrusion and refolding of two alpha-helical regions into transmembran

205 s suggest a possible mechanism for promoting refolding of Type III effectors after delivery into host

206 DnaK2 chaperone system, HtpG enhanced native refolding of urea-denatured lactate dehydrogenase and he

207 These results establish refolding of yeast chromosome III as a key driving force

208 manently folded but spontaneously unfold and refold on time scales that can span orders of magnitude

209 endent manner, and supports their productive refolding once nonstress conditions are restored.

210 proteins (HSPs), a family of chaperones that refold or degrade misfolded proteins.

211 site and may inhibit fusion by preventing F refolding or by blocking the F-HN interaction.

212 to dissociate aggregates and thereby enables refolding or degradation of misfolded proteins.

213 by unfolding aberrant and toxic proteins for refolding or proteolytic degradation.

214 f leaving after G4 unwinding, allowing it to refold, or going beyond to unwind duplex DNA, Pif1 repea

215 ng temperature and was footprinted along the refolding pathway using fast photochemical oxidation of

216 key conformational changes in the F-protein refolding pathway, but a detailed understanding of prefu

217 folding intermediates during its spontaneous refolding pathway.

218 this modelling indicates whether alternative refolding pathways might occur upon cooling.

219 While it is known that PS binds refolded pepsin and catalyzes its conversion to the nati

220 chanism is proposed for the binding of PS to refolded pepsin and how the formation of the native stru

221 cal shift data revealed that upon PS binding refolded pepsin folds into a state more similar to that

222 eractions between residues of prosegment and refolded pepsin lower the folding activation barrier.

223 Refolded pepsin was found to be partially structured and

224 the key equilibrium states, e.g. native and refolded pepsin, both in the free and PS-bound states, a

225 ally stable, non-native conformation, termed refolded pepsin, which is separated from native pepsin b

226 n (F) protein, the latter undergoing a major refolding process to merge the two membranes.

227 rotein, which undergoes a major irreversible refolding process to merge the two membranes.

228 suggests a kinetic coupling of the unfolding/refolding process with cis-trans prolyl isomerization.

229 to undergo a reversible pH-induced unfolding/refolding process, a loss/gain of alpha-helical structur

230 known about the intermediate states of the F refolding process.

231 The associated refolding processes often cannot be explained by thermod

232 progressively as the length is increased and refolds progressively as the length is reduced.

233 protein chaperone with well-defined peptide-refolding properties, is known to interact with ARE-like

234 stable misfolded polypeptides into natively refolded proteins.

235 sed cell-free protein synthesis and a simple refolding protocol.

236 dest yield but required the incorporation of refolding protocols to obtain a proper conformation.

237 cooperates with the DnaK chaperone system to refold purified luciferase.

238 deproteinized RNA isolated from cells versus refolded purified RNA suggests that the majority of the

239 allow the completely unfolded Luciferase to refold quickly in AFM experiments, strongly suggesting t

240 d-type proteins indicates the differences in refolding rates may be correlated with the degree of fru

241 through all-or-none transitions with similar refolding rates.

242 This indicates that the unfolding/refolding reaction is kinetically determined with differ

243 Here, we investigated the refolding reaction of ribonuclease T1 in the presence of

244 mutation in reconstituted disaggregation or refolding reactions in vitro.

245 This article discusses the structures and refolding reactions of specific fusion proteins and the

246 itiate thermodynamically favorable unfolding-refolding reactions that release the (DMA)C-labeled stra

247 rces, bringing the non-equilibrium unfolding-refolding reactions towards equilibrium.

248 Native BmAMA1 from parasite lysate and refolded recombinant BmAMA1 (rBmAMA1) expressed in Esche

249 at belong to the same supertype, and, during refolding, reduced aggregation of tapasin-independent al

250 e prevention of hinge movements in the first refolding region and the elimination of proteolytic expo

251 isfolds and how chaperones assist Luciferase refolding remains unknown.

252 he N-terminal helix of S-peptide unfolds and refolds repeatedly on the microsecond timescale, indicat

253 absence of divalent ions, NCS-1 unfolds and refolds reversibly in a two-state reaction involving onl

254 the N-terminal domain remaining folded, can refold robustly without chaperones.

255 with SDS, making it possible to release and refold SDS-denatured proteins by adding sufficient amoun

256 50 mus of all-atom molecular dynamics P-drop refolding simulations with four different force fields.

257 -type and adapted HIV-1 isolates, early gp41 refolding steps obligatorily occur on cell surfaces, whe

258 antibodies in engineered cells often require refolding steps or secretion across one or more biologic

259 and purified protein after simple oxidative refolding steps retained reduction-sensitive conformatio

260 showed an ATP-ase activity and an ability to refold substrates identical to the wild type.

261 cule atomic force microscopy (AFM) unfolding/refolding techniques to study the interactions of the UC

262 und motors toward the center of the cell, or refold the cargo-free motors into an inactive state read

263 crucial evidence for the ability of FtsH to refold the misassembled proteins.

264 their ability to suppress aggregation of and refold the model substrate gammad-crystallin, suppress a

265 ppress aggregation of mutant huntingtin, and refold the physiological substrate beta-actin in vitro.

266 /mol that represents the free energy cost of refolding the oligomeric intermediate into the structure

267 ism, whereby the beta-subunits independently refold, thereby templating the folding of the alpha-subu

268 The N-terminal 13 residues of CA refold to a beta-hairpin motif upon processing from its

269 branes together as two heptad-repeat regions refold to form a six-helix bundle structure that can be

270 Remarkably, both SpaA and FimA initially refold to mechanically weaker intermediates that recover

271 tein, to insert into the target membrane and refold to merge the viral and cellular membranes.

272 t approximately 68 to 70 degrees C but could refold to regain activity.

273 ntrinsic instability, cytosolic CTA1 must be refolded to achieve an active conformation.

274 ribosomal state to allow for mRNA structure refolding to drive large-scale ribosome movements.

275 ormational changes that energetically couple refolding to membrane fusion.

276 ase, we observe a slower 1.4-ms phase during refolding to the native state.

277 eins from the aggregates and assist in their refolding to the native state.

278 ts into the target membrane, trimerizes, and refolds to a hairpin-like conformation in which the comb

279 s F structures demonstrates that a conserved refolding trajectory mediates entry of these viruses and

280 dependent effect by increasing the amount of refolded type III collagen in vitro and FKBP19 seems to

281 her globular proteins unfolded by SDS can be refolded upon addition of C12E8 and DDM.

282 tion complex, or the RNA undergoes extensive refolding upon encapsidation.

283 events their aggregation, and supports their refolding upon subsequent neutralization.

284 of parainfluenza virus 5 F was triggered to refold using temperature and was footprinted along the r

285 uses the target domain to first unfold, then refold via domain swap.

286 ReFOLD was used to generate hundreds of alternative refi

287 Refolding was confirmed by ultrafast broadband transient

288 and hysteresis in the thermal unfolding and refolding was observed for all proteins.

289 ding states of cyt c in the early 500 mus of refolding was revealed on the microsecond time scale.

290 lling speeds (ca. 2 nm s(-1)), unfolding and refolding were observed to occur in near equilibrium.

291 The best conditions for refolding were optimized by a high throughput screening

292 Interestingly, the membranes cease to refold when spheroplasts lost viability, implying that t

293 iv) characterize the early stages of protein refolding when chemically denatured proteins are transfe

294 on the assumption of DSB-induced chromosome refolding, which also takes into account the previously

295 the DSB is induced chromosome III undergoes refolding, which directs the MAT locus to recombine with

296 jects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a se

297 molecules with cis and trans prolyl isomers refold with identical rates, because SlyD-catalyzed cis/

298 n this complex, the alpha subunits of Hb are refolded with the heme displaced to the interface with I

299 ) at loads <100 pN were accompanied by rapid refolding without either intra- or interhelix unfolding

300 he most beneficial factors for improving the refolding yield.