ライフサイエンスコーパス: refolding

1 95 kcals mol(-1) if 80% of A-B loop requires refolding).

2 gy transfer in the microsecond time range of refolding.

3 refolding, and, subsequently, again tertiary refolding.

4 everal times faster than that of spontaneous refolding.

5 ve regions of a protein chain and preventing refolding.

6 e mechanism of chaperone-assisted Luciferase refolding.

7 ve helical structure frustrating microsecond refolding.

8 rin cytoskeleton, including domain unfolding/refolding.

9 te structural dynamics with global unfolding/refolding.

10 ammaD-crystallin aggregation suppression and refolding.

11 trap as the reason for slower P-jump-induced refolding.

12 vitro assay for this step in fusion protein refolding.

13 an anticipated from models involving protein refolding.

14 from one another, leading to native protein refolding.

15 and ligand binding-dependent conformational refolding.

16 olded protein but did not affect the rate of refolding.

17 WF A2 stability and mechanical unfolding and refolding.

18 onformational changes that trigger F protein refolding.

19 at calcium stabilizes VWF A2 by accelerating refolding.

20 tercalates target membranes during F protein refolding.

21 G 91-108 epitope were greatly enhanced after refolding.

22 be important in regulating F triggering and refolding.

23 compact and extended states populated during refolding.

24 ion in bacteria followed by purification and refolding.

25 e of events could elucidate the mechanism of refolding.

26 s to undergo ligand-dependent conformational refolding.

27 ffinity purification and solid-phase protein refolding.

28 s that H223 protonation guards against early refolding.

29 protein aggregates, leading to their native refolding.

30 repeated rounds of stepwise G4-unfolding and refolding.

31 it C-terminus additionally assist in subunit refolding.

32 cient time for protein chaperones to attempt refolding.

33 agglutinin started to undergo conformational refolding.

34 1, to partner with Hsp70 in in vitro protein refolding.

35 transferred to ATP-dependent chaperones for refolding.

36 d using limited proteolysis and denaturation/refolding.

37 d) and the P3-strengthening mutations slowed refolding (6- to 1400-fold), suggesting that P3 indeed u

38 one mutation abolished both Hsc70 ATPase and refolding activities.

39 lts in significant impairment of its protein refolding activity in vitro without affecting interdomai

40 biquitous enzymes that stimulate the protein refolding activity of Hsp70 family chaperones.

41 a similar structure, ATP use, and substrate refolding activity, and, importantly, it also inhibits m

42 less than temperature denaturation, protein refolding after a fast P-jump is not necessarily faster

43 Upon refolding after chemical denaturation, this protein prod

44 ormational switch pathways for RNA hairpins: refolding after complete unfolding, folding through base

45 vinculin and vinculin binding inhibits talin refolding after force is released.

46 unfolding temperature but rather facilitated refolding after thermal stress.

47 However, in lieu of fostering their refolding, Aha1 allows ubiquitination of bound clients b

48 nins up to 80 degrees C, followed by partial refolding and aggregation at even higher temperatures.

49 ich, marginally stable oligomers in vitro on refolding and cross-beta-rich aggregates following incub

50 Allosteric effectors that mediate refolding and enhance enzymatic function have the potent

51 RiC and were active as assayed by luciferase refolding and human gammaD-crystallin aggregation suppre

52 apable of growing on the ends by seeding the refolding and incorporation of the normal form of the gi

53 ressed the mechanism by which H3 promotes E1 refolding and membrane fusion.

54 Both experimental refolding and Monte Carlo simulations of Markov state mo

55 ssary early step for paramyxovirus F-protein refolding and presents a novel target for structure-base

56 ess conditions, when both chaperone-mediated refolding and proteasomal degradation are compromised or

57 on in Escherichia coli followed by oxidative refolding and proteolysis.

58 activated beta-cardiac myosin is followed by refolding and reactivation of ATPase and motile activiti

59 is, including quality control during protein refolding and regulation of protein degradation.

60 hibited complex dynamics, including frequent refolding and state occupancies of <10 mus.

61 A164-A165 is a manifestation of the relative refolding and unfolding rate constants of each individua

62 tigens, this antigen preparation induces MR1 refolding and upregulates surface expression of human MR

63 We find little difference in ATPase, protein refolding, and amyloid inhibiting activities of purified

64 h display tertiary unfolding, then secondary refolding, and, subsequently, again tertiary refolding.

65 arance pathways involved in the recognition, refolding, and/or clearance of aberrant proteins.

66 The origin of this unfolding and refolding anisotropy is in the various numbers of native

67 oupling of direct genetic engineering with a refolding approach to produce an unusually stable protei

68 ed using typical dialysis- or dilution-based refolding approaches.

69 ionally folding protein whose extremely slow refolding (approximately days) is catalyzed by chaperone

70 The slowest steps in unfolding and refolding are N --> I1 (36 s(-1)) and MG --> I1 (26 s(-1

71 Interrupted refolding assays demonstrated that the two exponential p

72 e scFvs, the presence of Skp during in vitro refolding assays reduced aggregation but did not alter t

73 In vitro refolding assays under redox conditions showed that POFU

74 of the combinations in ATPase and luciferase refolding assays were dependent on the identity and stoi

75 phase, but also of a ~1.5 ms "slow" phase of refolding, attributed to non-native helical structure fr

76 anisotropy is also reflected in the complex refolding behavior of ARs.

77 Denaturation and refolding behavior of the allergen confirmed that its Ig

78 cium, the R1597W mutation slowed the rate of refolding but had no effect on unfolding.

79 amino acid foldon at the C terminus and slow refolding channeled gp41 into trimers.

80 to misfolded proteins and maintain them in a refolding competent state.

81 eir refolding, Hsp17.7 keeps substrates in a refolding-competent state by transient interactions.

82 by refolding is challenging because suitable refolding conditions must be empirically determined for

83 ere is no evidence for an intermediate under refolding conditions.

84 based determinations with respect to varying refolding conditions.

85 retained less activity than wild type after refolding, consistent with their reduced stability at lo

86 It is thought that F refolding couples the energy released with membrane fusi

87 nd fold rapidly with overlapping melting and refolding curves, G3T multimers (G3T units covalently at

88 activity prior to unfolding, but displayed a refolding defect resulting in reduced aminoacylation com

89 hypothesized that much like disaggregation, refolding, degradation, and even normal function, Hsc70

90 e resulting method, which we deem DSF guided refolding (DGR), thus enables the production of aggregat

91 chondrial proteins undergo partial unfolding/refolding during import, and both S57C and N280S variant

92 cessible means to characterize the unfolding/refolding dynamics of individual molecules and resolve c

93 cular chaperone Hsp90 is able to enhance the refolding efficiency of the model client protein firefly

94 ifferent kinetic constants for unfolding and refolding even though the process remains experimentally

95 (H or G) triggers F to undergo an extensive refolding event to form a stable postfusion state.

96 sion protein, F, which, through an extensive refolding event, brings viral and cellular membranes tog

97 insights into the mechanics of this critical refolding event.

98 stence of the sequential and the cooperative refolding events provides direct evidence for a unifying

99 Recent P-jump refolding experiments on the helix bundle lambda-repress

100 Single-molecule refolding experiments reveal the initial nucleation of f

101 When heat-denatured, the V domain enhanced refolding for all of the constructs; however, the foldin

102 In this study, we examine the mechanism of refolding for two distinct rhomboids to gain insight int

103 ition, the ability of the mutant to generate refolding forces is also reduced.

104 ered from the distributions of unfolding and refolding forces, allowing the diffusion constant for ba

105 ces the difference between the unfolding and refolding forces, bringing the non-equilibrium unfolding

106 oprotein obtained following denaturation and refolding forms a hexamer.

107 sion by inserting into a target membrane and refolding from a prefusion to postfusion conformation to

108 n-prone and disulfide-containing proteins by refolding from E. coli inclusion bodies, which would not

109 As F undergoes a dramatic refolding from its prefusion to postfusion conformation,

110 The P3-weakening mutations accelerated refolding from M (3- to 30-fold) and the P3-strengthenin

111 e P3-weakening mutations were larger than in refolding from M, and small-angle X-ray scattering showe

112 olding of native peripheral structure during refolding from M, which probably permits rearrangement o

113 cid-induced fusion, as well as insights into refolding from pre- to post-fusion conformations.

114 e helix-turn interfaces that should speed up refolding from the pressure-denatured state, if this hyp

115 e that FtsH contains the protease as well as refolding functions, and both the AAA and the proteolyti

116 ATP-dependent bacterial chaperones in their refolding, Hsp17.7 keeps substrates in a refolding-compe

117 Thus, pressure-jump (P-jump)-induced protein refolding, if it could be made fast enough, would be ide

118 a-repressor mutant is nonetheless capable of refolding in a single explicit solvent MD trajectory in

119 CYT-19 and Mss116p accelerate refolding in an ATP-dependent manner, presumably by disr

120 as measured by the inhibition of luciferase refolding in prostate cancer cells.

121 g high flexibility and dynamic unfolding and refolding in seconds or less.

122 ecules with identical domains misfold during refolding in vitro and form an unexpectedly stable state

123 ev1) disrupts G4 DNA structures and prevents refolding in vitro.

124 ve the kinetics of spontaneous unfolding and refolding in zero urea.

125 Here we report a rapid method for refolding inclusion-body-based, recombinant cell surface

126 ticity of mechanical proteins, unfolding and refolding individual proteins, probing protein-ligand in

127 We probed the formation of a refolding intermediate by time-resolved fluorescence ene

128 ibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies.

129 te into hairpin turns via a transient strand-refolding intermediate that involves DNA-base flipping a

130 nant virions efficiently and were capable of refolding into a postfusion conformation without tempora

131 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

132 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

133 rting into the target cell membrane and then refolding into a postfusion structure that fuses the vir

134 usogenic glycoproteins of these pathogens is refolding into a thermodynamically highly stable fusion

135 gement of both donor and target membrane and refolding into hairpin-like structures, have emerged as

136 he unfolded protein prevents rubredoxin from refolding into its native holo state.

137 ediate structures of the MACPF domain during refolding into the beta-barrel pore establish a structur

138 mers from the aggregate and their subsequent refolding into the native conformation.

139 The enzyme-catalyzed, multistep strand refolding is a novel mechanism in DNA rearrangement reac

140 In practice, however, protein production by refolding is challenging because suitable refolding cond

141 Loop refolding is limited by the hydrophobic collapse of the

142 Bax is kept inactive in the cytosol by refolding its C-terminal transmembrane domain into the h

143 e strain at which such fibers break, and the refolding kinetics and fraction of misfolded repeats.

144 NTE as influencing the chaperone-independent refolding kinetics and overall thermodynamic stability o

145 affect VWF A2 unfolding, but rather enhanced refolding kinetics fivefold, resulting in a 0.9 kcal/mol

146 The refolding kinetics of a bistable terminator antiterminat

147 we report the force-dependent unfolding and refolding kinetics of all talin rod domains.

148 of microsecond pressure and temperature jump refolding kinetics of the engineered WW domain FiP35, a

149 cond pressure-jump apparatus, we monitor the refolding kinetics of the helix-stabilized five-helix bu

150 asured the force dependence of unfolding and refolding kinetics.

151 of folding against force and accelerates the refolding kinetics.

152 n-random client proteins of the Hsp104/Hsp70-refolding machinery, including the prion Sup35.

153 partners and that context-dependent protein refolding may be widespread in nature.

154 ificial (betaalpha)(8)-barrels show the same refolding mechanism as HisF and other modern (betaalpha)

155 We conclude that the refolding mechanism of (betaalpha)(8)-barrel proteins is

156 ts demonstrate a cooperative, self-chaperone refolding mechanism, whereby the beta-subunits independe

157 Thus, activation or restoration of refolding mechanisms may alleviate TDP-43 aggregation in

158 BiP's ATPase activity, which is required for refolding misfolded proteins while coping with ER stress

159 oduces a kinetic barrier that partitions the refolding molecules to the nonpermuted structure.

160 our results suggest that initial Luciferase refolding occurs along a vectorial pathway and also sugg

161 In contrast to refolding of a denatured protein, cotranslational foldin

162 tight control over triggering the concerted refolding of a trimeric fusion protein.

163 nometre-scale rearrangement is controlled by refolding of an evolutionarily conserved 'tongue', which

164 contribution to the cooperativity comes from refolding of apo DHFR on binding the first ligand (up to

165 one were all unable to assist GroEL-mediated refolding of bacterial ribulose-bisphosphate carboxylase

166 f the SDS from the protein-SDS complexes and refolding of betaLG, BSA, and lysozyme, while alphaLA ch

167 Refolding of both proteins results in reassociation of t

168 f HMLalpha as the donor, when we perturb the refolding of chromosome III.

169 of Hsp90 interacts with disordered CTA1, and refolding of CTA1 by Hsp90 is dependent upon ATP hydroly

170 es a process that couples the Hsp90-mediated refolding of CTA1 with CTA1 extraction from the ER.

171 mc and also inhibits Cosmc-assisted in vitro refolding of denatured T-synthase.

172 Yet ARF6 alone did not promote the refolding of disordered CTA1 to an active state.

173 venting protein aggregation and facilitating refolding of dysfunctional proteins, critical to the sur

174 E1 fusion loop into the target membrane, and refolding of E1 to a stable trimeric hairpin conformatio

175 t of linker length on rates of unfolding and refolding of each protein domain.

176 (i) Env binding to CD4 and coreceptors; (ii) refolding of Env into the final 6-helix bundle structure

177 tained from stopped-flow measurements of the refolding of Escherichia coli adenylate kinase were anal

178 uble receptor to H is sufficient to initiate refolding of F, underscoring the physiological significa

179 ns between H and the F trimer and activating refolding of F.

180 other DNA polymerases, or hRev1 can prevent refolding of G4 DNA structures.

181 the activation energy required to cause the refolding of gB from a prefusion to a postfusion conform

182 ion pattern of gB, possibly representing the refolding of gB from its prefusion to its postfusion con

183 virus (EBV) is thought to be mediated by the refolding of glycoprotein B (gB) from a prefusion to a p

184 ical energy through mechanical unfolding and refolding of isopeptide bond-delimited polypeptide loops

185 ccompanied by dramatic all-alpha to all-beta refolding of its C-terminal domain.

186 ypeptides and mediate the out-of-cage native refolding of large proteins.

187 ging to observe the reversible unfolding and refolding of metalloproteins because of a loss or decomp

188 to chaperones, which aid in the clearance or refolding of misfolded proteins.

189 e native folding of nascent polypeptides and refolding of misfolded species, thereby buffering mutati

190 Therefore, we conclude that refolding of MOG increases its pathogenicity both by gen

191 for bacterial expression, purification, and refolding of murine p28.

192 At slow pulling speeds (<50 nm s(-1)), the refolding of NuG2 can be clearly observed.

193 biquitous chaperone is to participate in the refolding of proteins denatured by cytoplasmic stress, t

194 pid cofactors may facilitate the spontaneous refolding of PrP into an infectious form while also allo

195 rences between protein folding in the ER and refolding of purified proteins.

196 pe III secretion, although contribution from refolding of secreted proteins has not been ruled out.

197 ozymes could be restored by putative t2M/t4M refolding of stem secondary structure or tertiary bridgi

198 hat force-dependent stochastic unfolding and refolding of talin rod domains make talin a very effecti

199 Surprisingly, we find that during refolding of tandem repeats, independent of sequence ide

200 Defective refolding of TDP-43 is predicted to aggravate the TDP-43

201 rotein and RNA components requires extensive refolding of the 16S rRNA and is assisted by 10-20 assem

202 we propose the function of the maturational refolding of the beta-hairpin in CA assembly is to exten

203 ring, which enables reversible unfolding and refolding of the chains.

204 tute for DnaJ2 or GrpE in the DnaK2-assisted refolding of the denatured substrates.

205 During the hierarchical unfolding or refolding of the DNA complex, a 15-nucleotide hairpin se

206 Upon relaxation, refolding of the ferredoxin-like domains enables the hyd

207 diated GQ unfolding is typically followed by refolding of the GQ, a pattern that is repeated several

208 dependent conformational destabilization and refolding of the hole-hole homodimer Fc.

209 Here, the reversible unfolding-refolding of the iron-sulfur protein rubredoxin was obse

210 terized the molecular events associated with refolding of the metastable prefusion S glycoprotein to

211 Functional rescue was observed on refolding of the oligo-T/U strands into bPNA triplex hyb

212 unfolding, the motor domain interfered with refolding of the otherwise robust I27 modules, presumabl

213 er virus, we show that these changes involve refolding of the protein into a trimeric state.

214 and peptides suggests that SER5 also delays refolding of the remaining fusion-competent Env trimers.

215 This bridging action requires a complete refolding of the RfaH C-terminal domain (CTD) from an al

216 observed in the p53-MDM2 complex and induces refolding of the short, unstructured MDM2 N-terminal reg

217 otif affects only late intermediate R3, when refolding of the tongue and docking to the GAF1 domain a

218 Refolding of the U-loops into bPNA triplex stems complet

219 ified based on changes in the elasticity and refolding of the unfolded polyprotein in the presence of

220 dominant negative manner by interfering with refolding of the viral HR1 and HR2 to form a six-helix b

221 uggest that novel therapeutics enhancing the refolding of THP mutants may be of important value in th

222 pendent activation, titin elastic recoil and refolding of titin domains as an energy source, and Ca(2

223 e MACPF domain, accompanied by extrusion and refolding of two alpha-helical regions into transmembran

224 s suggest a possible mechanism for promoting refolding of Type III effectors after delivery into host

225 DnaK2 chaperone system, HtpG enhanced native refolding of urea-denatured lactate dehydrogenase and he

226 These results establish refolding of yeast chromosome III as a key driving force

227 But in addition, a striking effect of MOG refolding on the generation of T-cell responses was foun

228 endent manner, and supports their productive refolding once nonstress conditions are restored.

229 site and may inhibit fusion by preventing F refolding or by blocking the F-HN interaction.

230 to dissociate aggregates and thereby enables refolding or degradation of misfolded proteins.

231 te protein translation, and increase protein refolding or degradation.

232 by unfolding aberrant and toxic proteins for refolding or proteolytic degradation.

233 rates of terminal-strand extraction, strand refolding or recapture, and unfolding of the 10-stranded

234 ng temperature and was footprinted along the refolding pathway using fast photochemical oxidation of

235 key conformational changes in the F-protein refolding pathway, but a detailed understanding of prefu

236 folding intermediates during its spontaneous refolding pathway.

237 II, which binds to an intermediate in the E1 refolding pathway.

238 this modelling indicates whether alternative refolding pathways might occur upon cooling.

239 rotein, which undergoes a major irreversible refolding process to merge the two membranes.

240 n (F) protein, the latter undergoing a major refolding process to merge the two membranes.

241 suggests a kinetic coupling of the unfolding/refolding process with cis-trans prolyl isomerization.

242 to undergo a reversible pH-induced unfolding/refolding process, a loss/gain of alpha-helical structur

243 known about the intermediate states of the F refolding process.

244 that "crack" during unfolding and impede the refolding process.

245 onin function during the posttranslocational refolding process.

246 duce fully functional protein by an in vitro refolding process.

247 The associated refolding processes often cannot be explained by thermod

248 ighboring repeats depending on the unfolding/refolding propagation directions.

249 protein chaperone with well-defined peptide-refolding properties, is known to interact with ARE-like

250 sed cell-free protein synthesis and a simple refolding protocol.

251 dest yield but required the incorporation of refolding protocols to obtain a proper conformation.

252 e L427P mutated protein as well as a reduced refolding rate after denaturation.

253 d-type proteins indicates the differences in refolding rates may be correlated with the degree of fru

254 through all-or-none transitions with similar refolding rates.

255 This indicates that the unfolding/refolding reaction is kinetically determined with differ

256 Here, we investigated the refolding reaction of ribonuclease T1 in the presence of

257 mutation in reconstituted disaggregation or refolding reactions in vitro.

258 This article discusses the structures and refolding reactions of specific fusion proteins and the

259 itiate thermodynamically favorable unfolding-refolding reactions that release the (DMA)C-labeled stra

260 rces, bringing the non-equilibrium unfolding-refolding reactions towards equilibrium.

261 at belong to the same supertype, and, during refolding, reduced aggregation of tapasin-independent al

262 e prevention of hinge movements in the first refolding region and the elimination of proteolytic expo

263 isfolds and how chaperones assist Luciferase refolding remains unknown.

264 ive conformation thus requires unfolding and refolding, resulting in a long-lived intermediate.

265 kinetic analysis of human carbonic anhydrase refolding showed that 6AP decreased the yield of the ref

266 50 mus of all-atom molecular dynamics P-drop refolding simulations with four different force fields.

267 oxic forms of recombinant PrP after dilution refolding, size fractionation, and systematic biological

268 During the refolding step, misfolded proteins such as disulfide scr

269 -type and adapted HIV-1 isolates, early gp41 refolding steps obligatorily occur on cell surfaces, whe

270 antibodies in engineered cells often require refolding steps or secretion across one or more biologic

271 and purified protein after simple oxidative refolding steps retained reduction-sensitive conformatio

272 on is triggered but before completion of the refolding steps that drive the merging of the viral and

273 cule atomic force microscopy (AFM) unfolding/refolding techniques to study the interactions of the UC

274 /mol that represents the free energy cost of refolding the oligomeric intermediate into the structure

275 repared by denaturing methods and subsequent refolding; this facile approach for native RNA preparati

276 nce similarity but all are thought to act by refolding through a series of conformational intermediat

277 tored by decreasing the activation energy of refolding through introduction of a destabilizing mutati

278 hobic fusion loop into the cell membrane and refolding to a stable trimeric hairpin.

279 ribosomal state to allow for mRNA structure refolding to drive large-scale ribosome movements.

280 ormational changes that energetically couple refolding to membrane fusion.

281 ase, we observe a slower 1.4-ms phase during refolding to the native state.

282 eins from the aggregates and assist in their refolding to the native state.

283 region between the mechanical unfolding and refolding traces obtained by a laser-tweezers instrument

284 s F structures demonstrates that a conserved refolding trajectory mediates entry of these viruses and

285 6p as general RNA chaperones, and identify a refolding transition for further dissection of the roles

286 tion complex, or the RNA undergoes extensive refolding upon encapsidation.

287 events their aggregation, and supports their refolding upon subsequent neutralization.

288 function and determines the favorability of refolding versus degradation of Hsp90 client proteins.

289 Refolding was confirmed by ultrafast broadband transient

290 and hysteresis in the thermal unfolding and refolding was observed for all proteins.

291 ding states of cyt c in the early 500 mus of refolding was revealed on the microsecond time scale.

292 nts or urea; however, traditional methods of refolding were not successful in generating soluble form

293 lling speeds (ca. 2 nm s(-1)), unfolding and refolding were observed to occur in near equilibrium.

294 The best conditions for refolding were optimized by a high throughput screening

295 iv) characterize the early stages of protein refolding when chemically denatured proteins are transfe

296 on the assumption of DSB-induced chromosome refolding, which also takes into account the previously

297 the DSB is induced chromosome III undergoes refolding, which directs the MAT locus to recombine with

298 ) at loads <100 pN were accompanied by rapid refolding without either intra- or interhelix unfolding

299 physical property of a protein (in this case refolding) without affecting the corresponding enzymatic

300 he most beneficial factors for improving the refolding yield.