ライフサイエンスコーパス: renaturation

1 main bearing the proper conformation without renaturation.

2 ty undeterred by repetitive denaturation and renaturation.

3 otein to DNA has revealed a new mechanism of renaturation.

4 d aminoacylation compared to wild type after renaturation.

5 hydrolyzable analog of GTP, GMP-PCP, inhibit renaturation.

6 edure was developed for its purification and renaturation.

7 shown that hsp70 is required for luciferase renaturation.

8 rotein sequence or in vitro denaturation and renaturation.

9 e tested for additional factors that enhance renaturation.

10 ability to fibrillize by simple denaturation/renaturation.

11 olet-visible (UV-vis) monitored denaturation/renaturation.

12 s by denaturation followed by subunit mixing renaturation.

13 lectrochemically controlled denaturation and renaturation.

14 pressure for simultaneous solubilization and renaturation.

15 preventing their aggregation and spontaneous renaturation.

16 bind HA after SDS-PAGE, electrotransfer, and renaturation.

17 ons for NBD1-R expression, purification, and renaturation.

18 DNA strand-transfer and single-stranded DNA renaturation activities are associated with the unique N

19 s or less than that of hsp 70, and it has no renaturation activity in the absence of hsp 70.

20 This renaturation activity is greatly increased under conditi

21 tivated ATPase activity is essential for the renaturation activity of Hsp70; therefore, the effects o

22 N or the C terminus does not abolish the DNA renaturation activity of the protein.

23 e supplemented with a DnaJ protein to obtain renaturation activity.

24 -PAGE, and their identities were verified by renaturation and activity assays.

25 overed in biologically active form following renaturation and anion exchange chromatography.

26 ich blocked exchange mediated by spontaneous renaturation and branch migration; beta imposed a polari

27 of divalent cation concentration during mRNA renaturation and by the presence of an antisense sequenc

28 can be detected by their ability to undergo renaturation and catalyze the phosphorylation of a pepti

29 can be detected by their ability to undergo renaturation and catalyze the phosphorylation of a pepti

30 ablation, distamycin inhibition of binding, renaturation and competition experiments, and tissue dis

31 A c-Fos/c-Jun complex was generated by co-renaturation and purified via a His-tag on the full-leng

32 Renaturation and reconstitution experiments with Zn(2+)

33 t 95, 55, and 60 degrees C for denaturation, renaturation, and DNA extension, respectively.

34 t increase in the rate of in vitro substrate renaturation, and partial independence from the Hsp70 ch

35 the DNA template by thermal denaturation and renaturation, and this hairpin structure was predicted t

36 d stable presynaptic complexes that promoted renaturation as rapidly as RecA, but the recombinant hum

37 40 cooperate with further factors in protein renaturation, as demonstrated by the finding that lucife

38 omplementary single-stranded DNA and promote renaturation, as measured by Tris-phosphate agarose gel

39 n renatured by dialysis of the resin against renaturation buffer.

40 ed following electroelution from the gel and renaturation by dialysis.

41 protein family, which also enhances protein renaturation by hsp 70, we purified a 38-kDa protein fro

42 Although significant renaturation can occur with only hsp70 and DnaJ proteins

43 ve sequences follow a nonspecific pathway to renaturation consistent with a molecular "slithering" me

44 d be slightly induced by a urea denaturation-renaturation cycle in an extracellular protein fraction,

45 vity could be induced by a urea denaturation-renaturation cycle in this fraction.

46 Externally imposed denaturation-renaturation cycles did not reverse this tendency.

47 y by exploiting ion-induced DNA denaturation/renaturation cycles.

48 y, thus restoring stability, as evidenced by renaturation described previously and by 70-ns molecular

49 appropriate conditions for hybridization and renaturation, determinations of sequence complexity, and

50 The renaturation effect of ALA-D is additive with that of RF

51 The renaturation-enhancing effect of ALA-D may be caused by

52 Following electrophoresis and renaturation, enzymatic digestions of the substrate in 5

53 nzyme, following sequential denaturation and renaturation, estimate the molecular size of the catalyt

54 It is found that a typical renaturation event occurs through a nucleation step, whe

55 became protease-accessible, indicating rapid renaturation except along the nuclease fold with restora

56 ty through a five-step procedure including a renaturation experiment and found that its apparent mole

57 Denaturation/renaturation experiments in the presence of various meta

58 orption spectrum of E.NAD+ from denaturation-renaturation experiments reveals the presence of a broad

59 Renaturation experiments showed that the approximately 8

60 performed "double-jump" GdnHCl denaturation/renaturation experiments, with a third jump, where the r

61 ell defined folding intermediate in the acid renaturation folding pathway of this protein reflected i

62 nto a functional protein splicing element by renaturation from 6 M urea.

63 the synthetic peptide were reconstituted by renaturation from guanidinium chloride.

64 Temperature-induced unfolding and renaturation from the urea-denatured state revealed that

65 lving the formation of key base pairs before renaturation fully ensues.

66 g sequences could be restored, partially, by renaturation; however, self-cleavage of transcripts with

67 egation and to facilitate subsequent protein renaturation in cooperation with ATP-dependent chaperone

68 eaminases was observed on their denaturation-renaturation in EDTA, which revealed the mutants to be r

69 mycin (GA) on the kinetics of the luciferase renaturation in RRL were examined to gain insight into t

70 reversed GA-induced inhibition of luciferase renaturation in RRL.

71 rporated phosphate after denaturation and/or renaturation in SDS gels.

72 rporate a single nucleotide in situ upon its renaturation in the gel.

73 bind to DNA, but following denaturation and renaturation in the presence of DNA, binding can be demo

74 enuator could not be restored efficiently by renaturation in vitro.

75 The contribution of hsp90 to renaturation is only partially blocked by geldanamycin,

76 ion methods, controlled DNA denaturation and renaturation is particularly essential and achieved by c

77 g of rhodanese but, as with wild-type GroEL, renaturation is quenched by addition of mutant GroEL 'tr

78 However, renaturation is reduced under conditions that stabilize

79 es active proteins (without denaturation and renaturation), it is more sensitive compared with most e

80 o strains, G186AS and Downs, and analyzed by renaturation kinetics and genomic reconstruction with th

81 ents of single DNA molecule denaturation and renaturation kinetics in the presence of gp32 and its pr

82 enhancer glycerol (<5 m) does not alter the renaturation kinetics of EcMDH, it dramatically accelera

83 Here we use renaturation kinetics to compare the repetitive nature o

84 lasma capsulatum was determined by using DNA renaturation kinetics, genomic reconstruction, and flow

85 Conditions that promote renaturation of an unfolded protein also promote protein

86 A common obstacle to proper renaturation of an unfolded protein is aggregation, an i

87 The presence of free BACE Pro peptide during renaturation of BACE460 but not ProBACE460 increases rec

88 of 1 BchI:1 BchD was sufficient for maximum renaturation of BchD.

89 rmation without interfering with the correct renaturation of carbonic anhydrase.

90 , is a DNA binding protein that promotes the renaturation of complementary single strands.

91 inds to single-stranded DNA and promotes the renaturation of complementary single strands.

92 ichiometry of binding to DNA and the rate of renaturation of complementary strands were similar for t

93 cent, growing polypeptide chains to in vitro renaturation of complete chains and determining the role

94 ing anti-aggregation activity, promoting the renaturation of denatured proteins, and preferential bin

95 gation, also strongly inhibit EF-Tu-mediated renaturation of denatured rhodanese to levels near those

96 F) Tu has chaperone-like capacity to promote renaturation of denatured rhodanese.

97 The proposed model is used to examine the renaturation of DNA.

98 -induced attraction, which helps mediate the renaturation of DNA.

99 Renaturation of E1 with a similarly slow kinetics was al

100 mimicking double-strand breaks, and prevents renaturation of long stretches (>1 kb) of denatured dsDN

101 were tested for their ability to inhibit the renaturation of luciferase and bind to Hsp70 in reticulo

102 n of hsp 70, RF-hsp 70 increases the rate of renaturation of luciferase by hsp 70 about 3-4-fold.

103 Rabbit reticulocyte ALA-D stimulates the renaturation of luciferase by hsp 70 up to 10-fold at co

104 recycling protein and markedly enhances the renaturation of luciferase by hsp 70.

105 e lysate based upon its ability to stimulate renaturation of luciferase by hsp 70.

106 f Hsp70; therefore, the effects of HspBP1 on renaturation of luciferase in a reticulocyte lysate and

107 and peptide-K inhibited chaperone-dependent renaturation of luciferase in RRL.

108 Chaperone-mediated renaturation of luciferase obeyed Michaelis-Menten kinet

109 it dramatically accelerates the spontaneous renaturation of PmMDH at all temperatures tested.

110 Renaturation of polypeptides excised from sodium dodecyl

111 eterotrimers were formed by denaturation and renaturation of protein mixtures; when such mixtures con

112 We surveyed conditions for renaturation of purified rMMP-1 in 6 M guandine hydrochl

113 conformation and can be replicated by proper renaturation of recombinant Tromp1.

114 ependent denaturation followed by misaligned renaturation of repeated sequences and intermolecular pa

115 Renaturation of rhodanese and GTP hydrolysis by EF-Tu ar

116 related biological chaperones facilitate the renaturation of substrate proteins by minimizing the agg

117 with a short thermal denaturation, prevents renaturation of the duplex nucleic acids (dsDNA/RNA).

118 Further, denaturation/renaturation of the EICARMP-inactivated enzyme did not r

119 e recovery of activity upon denaturation and renaturation of the enzyme.I complex.

120 omplexes can be obtained by denaturation and renaturation of the fragment ("induced" association) wit

121 tent and fidelity of AMP-dependent, in vitro renaturation of the mutant AMP-free apoETF is reduced by

122 Isolation and renaturation of the protein from SDS/polyacrylamide gel

123 Renaturation of the small proteinase inhibitor cystatin

124 dC-tailed template assay, it was found that renaturation of the template was required for factor 2 f

125 Because the renaturation of the Venus fluorescent protein results in

126 Renaturation of thermally denatured firefly luciferase i

127 The renaturation of thermally denatured firefly luciferase r

128 However, the rates of denaturation and renaturation of V60N were faster.

129 Denaturation and renaturation of vitronectin isolated from human plasma w

130 Denaturation and renaturation of vitronectin under near physiological sol

131 MP-free apoETF is reduced by 57% compared to renaturation of wild type apoETF, likely due to the abse

132 ation of CAD by SDS gel electrophoresis, and renaturation on a nitrocellulose membrane, strongly sugg

133 espectively, following chemical denaturation/renaturation or transient heat shock.

134 at and/or boiling in SDS, precipitation, and renaturation or when fragments were recovered from an SD

135 ciation of cross-linked complexes by SDS and renaturation prior to immunoprecipitation did not preven

136 echnique and a SDS-polyacrylamide separation/renaturation procedure led to the hypothesis that this p

137 ctahedron structure by a simple denaturation-renaturation procedure.

138 determining step that limits the rate of the renaturation process and constitutes a kinetic trap.

139 A study of the denaturation-renaturation process of D(3) suggested that without this

140 DnaJ proteins, hsp90 also contributes to the renaturation process, both in the complex environment of

141 rated in vitro using a stepwise denaturation-renaturation process.

142 As renaturation progresses, the noncompact dimer undergoes

143 inactivate its capacity to chaperone protein renaturation, protected HRI from irreversible denaturati

144 f recombinant inclusion body protein using a renaturation protocol that was originally developed for

145 Prp21 was purified using a solubilization/renaturation protocol.

146 MfpA refolded using a novel "time-dependent renaturation" protocol yields protein with native second

147 eliminated by denaturation and recovered by renaturation, provided that the concentration of denatur

148 e strongly to a presumed intermediate in the renaturation reaction that beta itself catalyzed, and be

149 the polypeptide backbone; the inhibition of renaturation/reassociation described here is probably du

150 partitioning that slows the overall rate of renaturation relative to the chaperonin chamber, where s

151 sure of ProBACE460 to guanidine denaturation/renaturation results in a 7-fold higher recovery of BACE

152 y only if iron and sulfur are present in the renaturation steps.

153 For renaturation studies of recombinant Tromp1 (rTromp1), a

154 t hsp90 is not an essential component of the renaturation system.

155 By a fractionation/renaturation technique the Adx factor in mouse Y1 adreno

156 Kinase renaturation tests designed to detect reactivated protei

157 DNA but remains bound to the DNA product of renaturation that beta itself catalyzes.

158 Following renaturation, the 40-kD recombinant XCP1 was not proteol

159 xploit the fact that, following denaturation/renaturation, the PCR amplified JH flanking region DNA f

160 of alpha-crystallin was observed during the renaturation, the role of ATP is not clear.

161 Upon renaturation, this fragment bound to operator with an af

162 also undergoes autoproteolysis rapidly upon renaturation to produce two products.

163 The generality of "time-dependent renaturation" to other proteins and denaturation methods

164 The GA inhibited luciferase renaturation uncompetitively with respect to ATP concent

165 ntrol of five cycles of DNA denaturation and renaturation, under otherwise constant conditions, could

166 less effective than cyclodextrin at inducing renaturation upon detergent removal.

167 When renaturation was carried out in the absence of thiols, t

168 lded on the resin, and the efficiency of the renaturation was determined by the efficiency of the pro

169 ow rescue of protein from aggregation during renaturation, we developed a novel application of mass s

170 Conditions for its renaturation were determined by studying the refolding b

171 ycling, 30 cycles of pH-induced denaturation/renaturation were used to amplify protein DNA fragments

172 We have developed a technique based on DNA renaturation which normalizes repetitive DNA, and thereb

173 90-mediated p56lck biogenesis and luciferase renaturation while enforcing salt-stable interactions wi

174 HspBP1 inhibited renaturation with half-maximal inhibition at 2 microM.

175 Kinetic analysis of PmMDH renaturation with increasing glycerol concentrations sug

176 Improving renaturation yields by minimizing aggregation and reduci

177 of inclusion bodies whose solubilization and renaturation yields poly(A) polymerase activity that cor