ライフサイエンスコーパス: renature

1 istribution of states by which they denature/renature.

2 fragments were recovered from an SDS gel and renatured.

3 s suggests that some receptor PTPases can be renatured.

4 was purified under denaturing conditions and renatured.

5 d reconstitute nicked TyrR when added to the renatured 8-kDa fragment, as shown by physical propertie

6 of DnaJ proteins that are essential for the renaturing activity.

7 These polypeptides were renatured after elution from sodium dodecyl sulfate-gels

8 Product analysis by renaturing agarose gel electrophoresis after cross-linki

9 The protein was renatured and labeled while immobilized on the IMAC resi

10 Mouse KSR, like CAP kinase, renatures and autophosphorylates as a 100-kDa membrane-b

11 ly suspended in largely nonaqueous media but renatures and thus yields much higher activity if first

12 A procedure for renaturing and detecting the activity of protein tyrosin

13 content for the native, chemically denatured/renatured, and heat-shocked forms of GRP94.

14 The latter was recovered and renatured, and low-molecular-weight material was removed

15 as solubilized with guanidine hydrochloride, renatured, and purified by DEAE-Sephacel and Sephadex G-

16 hia coli, solubilized from inclusion bodies, renatured, and purified to homogeneity.

17 of its carbohydrate moieties, was extracted, renatured, and shown to have mismatch cutting specificit

18 Renatured apo-PITP demonstrated a significant relaxation

19 ified His(10)-hAR LBD in 6 M GdnHCl could be renatured as determined by ligand-binding activity, with

20 neutral pH; in method (B), the linkers were renatured at neutral pH and then added to D.

21 ated in dilute trifluoroacetic acid and then renatured at pH 7.8 and separated by chromatofocusing in

22 y Xie and Wetlaufer, who used this method to renature bovine carbonic anhydrase II.

23 e of PrP in a subset of samples that did not renature by the simple dilution of the GdnHCl.

24 mobilized metal affinity chromatography, and renatured by a two-step dialysis procedure and by a new

25 mmobilized on Co2+ charged Talon resin, then renatured by dialysis of the resin against renaturation

26 oteins to release substrates, which are then renatured by other larger heat shock proteins.

27 8 M urea by Ni-affinity chromatography, and renatured by rapid dilution at pH 5.

28 soluble, inactive inclusion bodies that were renatured by rapid dilution from 6 M urea.

29 sion bodies under denaturing conditions, and renatured by rapid dilution.

30 re solubilized, and the epitope fusions were renatured by slow dialysis.

31 -value is a constant, whereas under strongly renaturing conditions (<3 M), it depends on [C].

32 and DnaJ proteins function cooperatively to renature damaged proteins in the eukaryotic cytoplasm an

33 at (1) Cyt c can be reversibly denatured and renatured, depending on the urea concentration, and (2)

34 talyzed, and beta thereby protected all of a renatured duplex 83-mer oligonucleotide from nuclease di

35 The renatured enzyme is fully active and contains negligible

36 be fundamentally unstable as denaturing then renaturing experiments result in heterotrimeric/homotrim

37 l rods preferentially formed homodimers when renatured from an equimolar mixture of the two isoforms

38 th a spectrally native decorin that had been renatured from either 4 M guanidine hydrochloride by ext

39 recombinant leptin (re-leptin) purified and renatured from inclusion bodies in Escherichia coli.

40 n the inserted polypeptide is an intein, the renatured fusion protein can undergo protein splicing an

41 formation by renatured GFP-intein fusion and renatured GFP showed that under optimal conditions (pH 6

42 Comparison of chromophore formation by renatured GFP-intein fusion and renatured GFP showed tha

43 The mixture of renatured HisRecD protein and the RecB and RecC proteins

44 e tertiary structure, compared to native and renatured holo-PITP.

45 1 amino acid region of Rrp1, which binds and renatures homologous single-stranded DNA, does not share

46 ust be solubilized and the denatured protein renatured if an active molecule is to be recovered.

47 lectrophoresed and the separated PTPases are renatured in the gel by removing SDS with extensive wash

48 d rTromp1 was denatured in 8 M urea and then renatured in the presence of 0.5% Zwittergent 3,14 durin

49 purified on a metal ion affinity column and renatured in the presence of 2 mM ZnCl2.

50 the epitope if partially denatured and then renatured in the presence of PE specifically.

51 s-substituted CRP preparations denatured and renatured in the presence of various cAMP concentrations

52 Purified rat proCPA1 is renatured in vitro after denaturation in guanidine or in

53 mpared with mixtures of the two heavy chains renatured in vitro.

54 naturant allows the protein to spontaneously renature into fully functional 11-mers.

55 he P1 dimer with guanidine hydrochloride and renaturing it by dialysis, suggesting that the intramole

56 Dmc1 renatures linearized plasmid DNA with first order reacti

57 he fluorescent ATP analogue TNP-ATP bound to renatured NBD1-R with of 0.81 +/- 0.1 microM (wild-type)

58 ppaB dimers reconstituted from denatured and renatured p50 and p65 subunits from Ad12- and Ad5-transf

59 ination of the physicochemical properties of renatured p50 showed that it is a monomeric protein with

60 Outer membrane vesicles (OMV), recombinant renatured PorB (rrPorB), and PorB-expressing Venezuelan

61 eparin-binding affinities between native and renatured protein and invalidate the currently accepted

62 erone Ydj1p, with the Hsp70 class Ssa1p, can renature proteins.

63 d to detect 3'-phosphodiesterase activity of renatured proteins immobilized in polyacrylamide gels.

64 Assaying the activity of renatured PTPases after they have been separated by mole

65 loss in alpha-helical secondary structure of renatured rTromp1 compared to the nonrenatured form.

66 nic anhydrase protein standard (29 kDa), all renatured rTromp1 eluted at the position of the phosphor

67 Circular dichroism of nonrenatured and renatured rTromp1 showed a marked loss in alpha-helical

68 Renatured rTromp1 was passed through a Sephacryl S-300 g

69 n X-114 phase separations showed that all of renatured rTromp1, but none of nonrenatured rTromp1, pha

70 Finally, renatured rTromp1, but not the nonrenatured form, showed

71 nd then act cooperatively with other HSPs to renature the partially denatured protein in an ATP-depen

72 other molecular chaperones such as HSP70 to renature the protein and renew its biological activity.

73 It was not possible to renature the urea-treated 28-kDa fragment.

74 Therefore, we renatured the enzyme after SDS-polyacrylamide gel electr

75 s of enzymes in 1 M guanidinium chloride and renaturing them by removing the denaturant.

76 ns or isolated capsid proteins with urea and renaturing them by stepwise dialysis.

77 dicate that cellular PrP and PrP(27-30) both renature to a common structure that reconstitutes the gl

78 Y is very insoluble and can be denatured and renatured to regain activity only if iron and sulfur are

79 with guanidine hydrochloride at pH 3.5, and renaturing to form heteropolymeric 24-mers.

80 In contrast, purified CPA1 is not renatured under any of the conditions tested.

81 Trx1 has chaperone activity that renatures urea- or heat-denatured arginase back to the c

82 ding of heparin to both native and denatured/renatured vitronectin, methods for monitoring binding in

83 uate heparin binding to native and denatured/renatured vitronectin.