ライフサイエンスコーパス: replicase

1 ltransferase and helicase domains of the TMV replicase.

2 helper virus (HV) Tobacco mosaic virus (TMV) replicase.

3 imulating plus-strand synthesis by the viral replicase.

4 roteins as well as the assembly of the viral replicase.

5 not be used as templates by the tombusvirus replicase.

6 p is a novel component of the purified viral replicase.

7 e nonstructural proteins that form the viral replicase.

8 well as four major host proteins in the CNV replicase.

9 an important role in the assembly of the CNV replicase.

10 that is needed for the assembly of the viral replicase.

11 enzymes are shown to be interacting with the replicase.

12 teracts with nsp3a, a component of the viral replicase.

13 n inhibits the normal functions of the viral replicase.

14 tructures which can augment the abundance of replicases.

15 Here we show that protocells can select for replicases.

16 organization and inner workings of cellular replicases.

17 iderably more error-prone than high-fidelity replicases.

18 ciency and limited interference between both replicases.

19 eshold that is compatible with selecting for replicases.

20 ite of replication and assembly of the viral replicase, activities that are mediated by cis-acting RN

21 We explore various assumptions regarding replicase activity and protocell division.

22 FSBA inactivates HCV replicase activity in a concentration-dependent manner w

23 system can acquire useful functions such as replicase activity or the production of membrane compone

24 e equally benefits sequences with or without replicase activity.

25 p92(pol), with consequent inhibition of TBSV replicase activity.

26 eraction, NS5A hyperphosphorylation, and HCV replicase activity.

27 in this huge molecule that are essential to replicase activity.

28 survival, because they can block the cell's replicase and its ability to complete genome duplication

29 These caps harbored the PVX replicase and nonencapsidated vRNA and represented PD-an

30 wed that STMV CP promotes binding between HV-replicase and STMV RNA.

31 These viruses encode conserved replicase and structural proteins as well as more divers

32 Steady-state levels of PVX replicase and TGBp2 (which reside in the ER) proteins we

33 hat Polepsilon is the primary leading strand replicase and that Poldelta is restricted to replicating

34 Owing to their mutation-prone replicase and unique genome organization, IAV population

35 form for increasing processivity of cellular replicases and for coordinating various cellular pathway

36 s widely used in the expression of RNA virus replicases and represents a potential target for antivir

37 icase, (iii) the activity of the tombusvirus replicase, and (iv) the ratio of plus- versus minus-stra

38 s to transport infectious viral RNA, certain replicases, and certain structural proteins to neighbori

39 fectious viral machinery (viral RNA, certain replicases, and certain structural proteins) present in/

40 s of VRCs or VRCs in toto, we isolated viral replicase- and VRC-enriched fractions from TMV-infected

41 Bacterial replicases are complex, tripartite replicative machines.

42 Bacterial replicases are highly processive, yet cycle rapidly duri

43 Chromosomal replicases are multicomponent machines that copy DNA wit

44 ive than wt NS5B in cell-free polymerase and replicase assays.

45 We used an in vitro replicase assembly assay based on yeast cell extract and

46 The cis-elements for replicase assembly can partially overlap with RNA recrui

47 is-acting viral RNA sequences that stimulate replicase assembly, we performed a systematic deletion a

48 NS2 is not required for NS2-3 processing and replicase assembly.

49 ed the B box, which is also critical for the replicase assembly.

50 in template recruitment into replication and replicase assembly; however, the importance of each of t

51 f the structural protein M and nonstructural replicase-associated proteins nsp1 and nsp13, which are

52 s at the active site of the C-family Pol III replicase at a step that does not require correct base-p

53 en together, these findings suggest that the replicase-ATAF2 interaction suppresses basal host defens

54 In this study, a similar replicase-Aux/IAA interaction affecting disease developm

55 Combined, these findings suggest that TMV replicase-Aux/IAA interactions selectively enhance virus

56 sponding genome counterpart to provide viral replicase (B1+B2+B3/FCP and F1+F2/BCP) resulted in the e

57 on presumably blocks progression of cellular replicases because the N3-methyl group hinders interacti

58 dium, natural selection would not favor such replicases because their presence equally benefits seque

59 ORF2p is the L1 replicase, but the role of ORF1p is unknown.

60 mulation and the activity of the tombusvirus replicase by up to fivefold.

61 The coronavirus replicase carries out regulatory tasks involved in the m

62 id composition, nucleotide similarities, and replicase catalytic domain location contributed to phylo

63 ing strand based in its interaction with the replicase chi-subunit.

64 s based on the translational operator of the replicase cistron, a 19 nt fragment (TR).

65 on, indicating that Xrn1 decay and the viral replicase compete to set RNA abundance within infected c

66 the minus strand is synthesized by the viral replicase complex (VRC), which then serves as a template

67 mbrane-bound structures containing the viral replicase complex (VRC).

68 s are replicated by the membrane-bound viral replicase complex (VRC).

69 f several co-opted host factors in the viral replicase complex (VRC).

70 ation proteins and is recruited to the viral replicase complex (VRC).

71 eins and inhibited the assembly of the viral replicase complex and viral RNA synthesis in vitro.

72 on in the localization of genomic RNA to the replicase complex at an early stage of infection.

73 y provide a platform for the assembly of the replicase complex consisting of viral and host proteins.

74 The tombusvirus replicase complex contains heat shock protein 70 (Hsp70)

75 e addition and can be used directly to study replicase complex formation and evolution during infecti

76 II proteins and the viral RNA in tombusvirus replicase complex formation using in vitro, yeast-based,

77 tate the recruitment of GAPDH into the viral replicase complex in the yeast model host.

78 However, its exact role within the replicase complex is still not clear.

79 Here, we studied the in vivo primary replicase complex more in-depth to determine the minimum

80 nt metabolic enzyme that is recruited to the replicase complex of Tomato bushy stunt virus (TBSV) and

81 ly from nonplant sources, a fully functional replicase complex of Tomato bushy stunt virus (TBSV).

82 and inner layer made of VP3 that encloses a replicase complex of VP1, VP4, and VP6 and a genome of 1

83 The assembly of the replicase complex required the heat shock protein 70 (Hs

84 pyruvate kinase (PK) directly into the viral replicase complex to boost progeny RNA synthesis.

85 l RNA recruitment, the assembly of the viral replicase complex, and viral RNA synthesis.

86 During the assembly of the membrane-bound replicase complex, the viral RdRp becomes activated thro

87 ary replication but appears to stabilize the replicase complex.

88 translocator during assembly of the primary replicase complex.

89 ication and assembly of the functional viral replicase complex.

90 so necessary for assembly of an active viral replicase complex.

91 to be critical for the assembly of the viral replicase complex.

92 uld not be trans complemented by a wild-type replicase complex.

93 r ORF3 in orchestrating the formation of the replicase complex.

94 emonstrate that the in vitro assembled viral replicase complexes (VRCs) in artificial PE vesicles can

95 dRp) and regulation of the assembly of viral replicase complexes (VRCs).

96 n, some are recruited to improvise the viral replicase complexes for genome multiplication, and other

97 icase, we affinity purified functional viral replicase complexes from yeast.

98 A viruses, replicate in membrane-bound viral replicase complexes in the cytoplasm of infected cells.

99 replication occurs via the assembly of viral replicase complexes involving multiple viral and host pr

100 They also inhibit formation of functional replicase complexes, but have no activity against prefor

101 es past dUs, even in the presence of stalled replicase complexes, thus providing a mechanism for main

102 GuHCl, as well as the components of the MRV replicase complexes.

103 normal viral factories containing functional replicase complexes.

104 hat is proteolytically self cleaved into two replicase components involved in viral RNA replication.

105 plication by preventing expression of mature replicase components.

106 and the NS5B polymerase, membrane-bound HCV replicase components.

107 s with the papain-like protease and putative replicase components: RdRp, methylase and helicase.

108 Bacterial DNA replicases contain multiple subunits that change interac

109 Cellular replicases contain multiprotein ATPases that load slidin

110 The activity of the purified CNV replicase correlated with viral RNA replication in the a

111 s, suggesting that assembly of TBSV and CIRV replicases could take place in the purified ER and mitoc

112 ion 3 in the N-terminal 13aa motif abolished replicase-CP binding.

113 he production of new plus strands, the viral replicase displaces the old plus strand in the dsRNA tem

114 The activity of the mitochondrial replicase, DNA polymerase gamma (Pol gamma) is stimulate

115 components or subassemblies of the bacterial replicase, DNA polymerase III holoenzyme (Pol III HE).

116 , which functions as a sliding clamp for the replicase, DNA polymerase III holoenzyme.

117 ding of the beta-clamp subunit (DnaN) of the replicase, DnaA is inactivated as its intrinsic ATPase a

118 Lastly, we demonstrate that the HCV replicase does not colocalize with autophagosomes, sugge

119 due to end-to-end template switching by BMV replicase during (-)-strand synthesis.

120 mavirus (HPV) genomes requires the viral DNA replicase E1.

121 The BMV replicase enzyme supported a lower recombination frequen

122 ncoded capsid proteins to downstream-encoded replicase enzymes.

123 readthrough to regulate expression of their replicase enzymes.

124 We found that replicase error rates vary by fork direction, coding sta

125 nses gap filling to modulate affinity of the replicase for the template, we performed photo-cross-lin

126 vivo requirement for two DNA polymerase III replicases for B. subtilis chromosomal replication, both

127 ese co-opted cellular factors in tombusvirus replicase formation.

128 nd as a checkpoint protein that prevents the replicase from advancing in a strand displacement reacti

129 ologous end-joining machinery and a putative replicase from an archaeal plasmid have shown striking f

130 n vitro activity of the purified tombusvirus replicase from gef1Delta yeast was low and that the in v

131 ic viral transcripts expressing an authentic replicase from open reading frame 2 (ORF2) and a second

132 mpounds that inhibit only a single bacterial replicase from those that exhibit broad spectrum potenti

133 By performing assays against replicases from model Gram-negative and Gram-positive ba

134 itness is not due to defective polymerase or replicase function and is more likely to result from the

135 This intramolecular compensation for the HCV replicase function by amino acid changes in different do

136 We demonstrate that PriA blocks replicase function on forks by blocking its binding.

137 the C-terminal domains are important for HCV replicase function.

138 that nsp10 is a major regulator of SARS-CoV replicase function.

139 ive clamp loaders, but tau confers important replicase functions including chaperoning the polymerase

140 2 or 3 is tolerated and that these reporter-replicase fusions can be used to quantitate replication

141 ting the amino-terminal end of the HCoV-NL63 replicase gene and established protease cis-cleavage ass

142 RNA structure that maps to the region of the replicase gene encoding the nonstructural protein 15 sub

143 The results show that the replicase gene has flexibility to accommodate a foreign

144 se results identify a new cistron in the MHV replicase gene locus and show that nsp3 has an essential

145 s a new complementation group within the MHV replicase gene locus.

146 or specific interactions between coronavirus replicase gene products and a cis-acting genomic RNA ele

147 Here, we identify HCoV-NL63 replicase gene products and characterize two viral papai

148 been demonstrated; however, the role of the replicase gene remains unexplored.

149 We demonstrate that the replicase gene tolerates the introduction of green fluor

150 between two in-frame NotI sites in the P150 replicase gene, a deletion encompassing nucleotides 1685

151 ing the structural proteins, rather than the replicase gene, that determines the pathogenic propertie

152 ernative reading frame overlapping the viral replicase gene.

153 9/JHM recombinant viruses indicates that the replicase genes are interchangeable and that it is the 3

154 We assessed the influence of the replicase genes of the highly neurovirulent MHV-JHM stra

155 We found that the CMV replicase had different fidelity in different environmen

156 hat the clamp loader of the Escherichia coli replicase has the composition DnaX3deltadelta'chipsi.

157 Some sequences - so-called replicases - have enzymatic activity in the sense of enh

158 sed inhibitors and by characterizing them in replicase HCV protease-resistant mutants assay.

159 ratio between p33 and p92(pol) in the viral replicase, (iii) the activity of the tombusvirus replica

160 uman DNA pol gamma, the heterotrimeric mtDNA replicase implicated in certain mitochondrial diseases a

161 and that the in vitro assembly of the viral replicase in a cell extract was inhibited by the cytosol

162 drial DNA polymerase (Pol gamma) is the sole replicase in mitochondria.

163 fonyl)benzoyl]adenosine (FSBA) to modify HCV replicase in order to identify the ATP binding site in t

164 Altogether, tombusvirus replicase in the cell-free system showed features remark

165 the presence of non-phosphorylated VP30 or a replicase in the presence of phosphorylated VP30.

166 Moreover, when copied in the BMV replicase in vitro reaction, the minus-strand RNA3 templ

167 Cellular replicases include three subassemblies: a DNA polymerase

168 s remarkably similar to those of the in vivo replicase, including carrying out a complete cycle of re

169 hypothesize that a physical interaction with replicase increases the CP specificity for packaging vir

170 The HCV replicase inhibitor 2'C-methyl adenosine was used to dis

171 convergent synthesis of the prodrugs of HCV replicase inhibitors 1-5 is described.

172 We identified that the STMV CP-HV replicase interaction requires a positively charged resi

173 nsp6-specific peptide antiserum detected the replicase intermediate p150 (nsp4 to nsp11) and two nsp6

174 The hepatitis C virus (HCV) NS5B replicase is a prime target for the development of direc

175 eaves, we show that brome mosaic virus (BMV) replicase is competent to initiate positive-strand [(+)-

176 a template for (+)RNA synthesis by the viral replicase is facilitated by recruited host DEAD box heli

177 Furthermore, the trimeric replicase is fully functional at a replication fork with

178 Thus, the typical, wild-type HCV replicase is uniquely regulated by lipid peroxidation, p

179 so inhibited RNA synthesis by the native HCV replicase isolated from HCV replicon cells and the recom

180 DnaE does not serve as the lagging strand replicase, like DNA polymerase delta in eukaryotes, but

181 cation through proteolytic processing of RNA replicase machinery.

182 e found extensive co-localization of the HCV replicase markers NS5A and double-stranded RNA with Rab5

183 he role of RNA structure and proteins in the replicase-mediated copy-choice mechanism.

184 vious results had suggested that the E. coli replicase might play a role in lesion bypass, but this p

185 s of an Okazaki fragment, the lagging strand replicase must recycle to the next primer at the replica

186 The N terminus of the replicase nonstructural protein 2 (nsp2) of porcine repr

187 has identified putative ts mutations in the replicase nonstructural proteins (nsp's) of these corona

188 tisera directed against two of the predicted replicase nonstructural proteins (nsp3 and nsp4) and det

189 nally processed into intermediate and mature replicase nonstructural proteins (nsps).

190 ither p150, the precursor polypeptide of the replicase nonstructural proteins nsp4 to nsp10, or the r

191 amino acid substitutions, including 4 in the replicase (nsp1, nsp2, nsp7, and nsp9) and 12 in the str

192 The alpha subunit of the replicase of all bacteria contains a php domain, initial

193 The replicase of all cells is thought to utilize two DNA pol

194 e of insertion and deletion mutations of the replicase of Cucumber mosaic virus (CMV) was determined

195 y exchange with the polymerase III (Pol III) replicase on the beta-clamp and function with DnaB helic

196 ) ions on the in vitro assembly of the viral replicase, on the activity of the viral RNA-dependent RN

197 erase domain generated a polymerase favoring replicase over transcriptase activity, providing strong

198 is fully nested within the ORF of the viral replicase P.

199 The assembled TBSV replicase performed a complete replication cycle, synthe

200 accurately bypass this adduct, while Pol III replicase, Pol IV, and Pol V were strongly inhibited.

201 that MGME1 interacts with the mitochondrial replicase PolgA, suggesting that it is a constituent of

202 Pol rate generalizes to B-family eukaryotic replicases, Pols delta and epsilon.

203 -CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a

204 porter molecules could be expressed from the replicase polyprotein of murine hepatitis virus as fusio

205 s expressed from native locations within the replicase polyprotein of murine hepatitis virus as fusio

206 The processing of replicase polyprotein pp1a in the region of nsp1 to nsp3

207 nonstructural proteins nsp4 to nsp10, or the replicase polyprotein pp1ab to produce nsp12.

208 ine protease domain within the nonstructural replicase polyprotein precursor, is responsible for the

209 irst strategy, a mutant was created in which replicase polyprotein translation initiated with nsp3, t

210 ke protease (PLP), which processes the viral replicase polyprotein, has deubiquitinating (DUB) activi

211 LPs), PLP1 and PLP2, which process the viral replicase polyprotein.

212 delay in proteolytic processing of the viral replicase polyprotein.

213 The first open reading frame encodes replicase polyproteins 1a and 1ab, which are cleaved to

214 lication by cleaving a site within the viral replicase polyproteins and also removes ubiquitin from c

215 Coronavirus replicase polyproteins are translated from the genomic p

216 ke protease (PL(pro)) that cleaves the viral replicase polyproteins at three sites releasing non-stru

217 positive-stranded RNA viruses) express their replicase polyproteins pp1a and pp1ab from two long ORFs

218 ndrome coronavirus (SARS-CoV) is mediated by replicase polyproteins that are processed by two viral p

219 Coronaviruses encode large replicase polyproteins which are proteolytically process

220 xtensive proteolytic processing of the large replicase polyproteins, pp1a and pp1ab, by two types of

221 ole in the proteolytic cleavage of the PRRSV replicase polyproteins.

222 lity of specific phenotypes; error-prone DNA replicases produce bursts of variability in times of str

223 The identification of the replicase products and characterization of HCoV-NL63 PLP

224 We found that HCoV-NL63 replicase products can be detected at 24 h postinfection

225 Mouse hepatitis virus (MHV) replicase products nsp3, nsp4, and nsp6 are predicted to

226 ent with its proposed role in a multiprotein replicase-proofreading complex.

227 we recently showed that multifunctional FHV replicase protein A induces viral RNA template recruitme

228 evaluating the molecular interaction between replicase protein and CP using a FHV-Nicotiana benthamia

229 con-transfected C-Vero cells, C and the P150 replicase protein associated by coimmunoprecipitation, s

230 The data suggest that replicase protein interactions directly or indirectly re

231 The nsp2 replicase protein of porcine reproductive and respirator

232 The replicase protein of Tobacco mosaic virus (TMV) disrupts

233 the Tobacco mosaic virus (TMV) 126-/183-kDa replicase protein(s) and the Arabidopsis thaliana NAC do

234 bacco mosaic virus (TMV) 126- and/or 183-kDa replicase protein(s) was found to interact with the Arab

235 a lethal deletion (termed deltaNotI) in P150 replicase protein.

236 noprecipitation assays demonstrated that FHV replicase (protein A) and CP physically interact at the

237 sis revealed the presence of CNV p33 and p92 replicase proteins as well as four major host proteins i

238 could define the expression and targeting of replicase proteins during infection in live cells.

239 s could define the targeting and activity of replicase proteins during infection.

240 Thus, the timing and targeting of native replicase proteins expressed in real time from native lo

241 The replicase proteins form membranous compartments in cells

242 ctural proteins (nsp3 and nsp4) and detected replicase proteins from HCoV-NL63-infected LLC-MK2 cells

243 This result was noteworthy, since viral replicase proteins have seldom been described in direct

244 ionally, live-imaging studies of coronavirus replicase proteins have used fluorescent reporter molecu

245 g exonuclease (nsp14-ExoN), as well as other replicase proteins involved in regulation of fidelity.

246 The possible involvement of replicase proteins is an example.

247 HM.WU structural protein M and nonstructural replicase proteins nsp1 and nsp13 are essential for live

248 NA) of Cucumber Mosaic Virus is dependent on replicase proteins of helper virus (HV).

249 The capsid and replicase proteins of PCV3 are only 37% and 55% identica

250 Multiple small circular DNA genomes encoding replicase proteins plus two highly divergent members of

251 are thought to serve as cofactors with other replicase proteins to assemble a larger replication comp

252 used fluorescent reporter molecules fused to replicase proteins, but expressed from nonnative locatio

253 membrane fractions, which contain the viral replicase proteins, in cells with replicating HCV.

254 resulted in aberrant agglomeration of viral replicase proteins, including NS5A, NS5B, and NS3.

255 These create a platform to concentrate replicase proteins, virus genomes and host proteins requ

256 rotein or firefly luciferase as fusions with replicase proteins.

257 the RC and alters the colocalization of HCV replicase proteins.

258 tiple point mutations in their nonstructural replicase proteins.

259 RNA3 templates was copied with either a BMV replicase (RdRp) preparation or recombinant BMV protein

260 that are important for the first step of the replicase reaction: the ATP-dependent formation of an in

261 of a peptide substrate that mimics the PLpro replicase recognition sequence.

262 The formation of the TBSV replicase required two purified recombinant TBSV replica

263 ) present in the php domain of all bacterial replicases resulted in defects in epsilon binding.

264 An RNA replicase ribozyme has long been sought by chemists inte

265 ates, are necessary; accordingly the minimal replicase ribozyme may have possessed restriction functi

266 However, how such replicase ribozymes emerged from the pools of short RNA

267 ion of the yeast extract, in which the viral replicase-RNA complex became RNase- and proteinase-resis

268 n a membraneous fraction, in which the viral replicase-RNA complex was RNase and protease resistant b

269 DNA replicases routinely stall at lesions encountered on the

270 Although it is well documented that archaeal replicases specifically arrest at deoxyuracils (dUs) due

271 that the SARS-CoV proteome contains several replicase, structural, and accessory proteins that antag

272 ed by a protein factor, specifically a PRRSV replicase subunit (nsp1beta).

273 for a trans-acting protein factor, the viral replicase subunit nsp1beta.

274 tions were mapped to the amino terminus of a replicase subunit, nonstructural protein 3 (nsp3).

275 We compared individual S. aureus replicase subunits with subunits from the Gram-negative

276 hogens, the helicase domain (p50) of the TMV replicase, the avirulence gene of N, was linked to synth

277 exes, but have no activity against preformed replicase, thereby resulting in slow shut-off of viral R

278 The ability of the TMV replicase to interact with Aux/IAA proteins from diverse

279 The in vitro assembly of the replicase took place in the membraneous fraction of the

280 to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of

281 N protein associates with a component of the replicase-transcriptase complex, nonstructural protein 3

282 tween N and the largest subunit of the viral replicase-transcriptase complex, nonstructural protein 3

283 The largest component of the coronavirus replicase-transcriptase complex, nsp3, contains multiple

284 onstructural protein 15 subunit of the viral replicase-transcriptase complex.

285 se hepatitis virus (MHV) is carried out by a replicase-transcriptase composed of 16 nonstructural pro

286 s of RNA oligonucleotides that encompass the replicase translational operator stem-loop of the RNA ba

287 Pol delta, like bacterial replicases, undergoes collision release upon completing

288 ns from the NS3-NS5B polyprotein to create a replicase unit for replication of its genome.

289 t in the cucumber necrosis tombusvirus (CNV) replicase, we affinity purified functional viral replica

290 n insights into the functions of a viral RNA replicase, we have assembled in vitro and entirely from

291 re of the (-)RNA in the membrane-bound viral replicase, we performed complete RNA replication of Toma

292 tions in the structural proteins but not the replicase were responsible for the establishment of pers

293 exhibit much lower fidelity than the cell's replicase when copying normal DNA, which results in a dr

294 in the in vitro activity of the tombusvirus replicase when isolated from APB-treated yeast.

295 unexpected assembly of the mitochondrial DNA replicase where the catalytic subunit Pol gammaA interac

296 of several cellular proteins into the viral replicase, which otherwise play proviral roles in replic

297 Pol III holoenzyme is the cell's main replicase, while pol I is responsible for the maturation

298 ruses [(+)RNA viruses] is performed by viral replicases, whose function is affected by many cellular

299 nants generated by template switching of BMV replicase with a nascent UTR from WT RNA1 or RNA2 during

300 Complementation with homologous replicase (with respect to CP) failed to enhance packagi