ライフサイエンスコーパス: replication initiation

1 e.g., sterol biosynthesis) or not (e.g., DNA replication initiation).

2 ., constant growth increments) that start at replication initiation.

3 bly of the GINS complex but is essential for replication initiation.

4 repair in addition to its essential role in replication initiation.

5 hereupon a dihexamer forms, resulting in DNA replication initiation.

6 recruitment during reactivation to preclude replication initiation.

7 ng partners on chromatin and facilitates DNA replication initiation.

8 ents lead to reverse transcription and viral replication initiation.

9 g cell physiology, separate from its role in replication initiation.

10 es adjacent to one side of the major site of replication initiation.

11 45 and GINS helicase-activating proteins and replication initiation.

12 ycle exit by functioning at a step after DNA replication initiation.

13 ity may be a common mechanism for regulating replication initiation.

14 o the molecular mechanisms of eukaryotic DNA replication initiation.

15 by cyclin-dependent kinases is essential for replication initiation.

16 ucidated concerning their functional role in replication initiation.

17 omplexes of these proteins are necessary for replication initiation.

18 lows origin licensing and prevents premature replication initiation.

19 e dnaA promoter to control the timing of DNA replication initiation.

20 ggesting that LPS phosphorylation stimulates replication initiation.

21 tin structure plays an important role in DNA replication initiation.

22 case is the committed step in eukaryotic DNA replication initiation.

23 Mcm10 are factors that are also required for replication initiation.

24 ng the viability of mutants defective in DNA replication initiation.

25 possibly be independent from its role in DNA replication initiation.

26 effects of a primary activity regulating DNA replication initiation.

27 is the focus of several pathways to control replication initiation.

28 allows Cdc45 loading and derepression of DNA replication initiation.

29 of ParA halts segregation without affecting replication initiation.

30 for origin licensing, a prerequisite for DNA replication initiation.

31 BNA1 at sites of active transcription or DNA replication initiation.

32 ion Complex (ORC) is a critical component of replication initiation.

33 idly form oligomeric complexes competent for replication initiation.

34 ith DNA are necessary for iteron binding and replication initiation.

35 lleles of dnaA that reduce the efficiency of replication initiation.

36 proteins may play similar roles in archaeal replication initiation.

37 chastic, leading to a random distribution of replication initiation.

38 CD2 reduces the number of potential sites of replication initiation.

39 e a common G-rich motif and exhibit elevated replication initiation.

40 ive an analytical expression for the rate of replication initiation.

41 ouble hexamer that facilitates bidirectional replication initiation.

42 ts of the pre-replication complex during DNA replication initiation.

43 howed that AZ-27 inhibited transcription and replication initiation.

44 synthesis and shortens S phase by increasing replication initiation.

45 genes cause disruption of the origin of DNA replication initiation.

46 -phase and define potential locations of DNA replication initiation.

47 cells imparts a rapid and complete block to replication initiation.

48 helicases that are central to bidirectional replication initiation.

49 changes, which are likely incompatible with replication initiation.

50 reaction that exhibits hallmarks of cellular replication initiation.

51 orylation, each previously shown to regulate replication initiation.

52 parapsilosis also employs recombination for replication initiation.

53 ediate chromosome segregation independent of replication initiation.

54 our understanding of mechanisms that control replication initiation.

55 oordinated to ensure an even distribution of replication initiation across the genome.

56 scuss here the molecular choreography of DNA replication initiation across the three domains of life,

57 c2 or Cdt1 suffice to recruit all downstream replication initiation activities to a prospective origi

58 xRTS accumulates on chromatin during replication initiation, after prereplication-complex (pr

59 se cyclin-dependent kinases (CDKs) stimulate replication initiation and accelerate progression throug

60 etabolic complex is enriched at sites of DNA replication initiation and associated with proliferating

61 DDK plays essential roles in DNA replication initiation and checkpoint activation.

62 We used RNAi against Cdc7 kinase to inhibit replication initiation and dissect this checkpoint in fi

63 e helicase 4 (RECQL4) plays crucial roles in replication initiation and DNA repair; however, the cont

64 This enzyme is implicated in replication initiation and DNA topology restoration and

65 Thus, human Mcm10 regulates both replication initiation and elongation and maintains geno

66 se, consistent with the observation that DNA replication initiation and elongation are impaired in so

67 To investigate the contribution of DNA replication initiation and elongation to the intra-S-pha

68 we establish that RbN directly inhibits DNA replication initiation and elongation using a bipartite

69 arily conserved factor that is essential for replication initiation and elongation.

70 dimer is required both for activation of DNA replication initiation and for interaction with Spo0J.

71 ell cycle-controlled, peaking at the time of replication initiation and gcrA induction.

72 ct relationship between sites of reduced DNA replication initiation and genetic damage.

73 phed mechanisms, often through the action of replication initiation and helicase-loader proteins.

74 lication stress is caused by deregulation of replication initiation and increased interference betwee

75 s in plasmid origins and participate both in replication initiation and its control.

76 Here, we review our current understanding of replication initiation and its regulation, describe mech

77 mbly pathways is discussed in the context of replication initiation and lagging strand synthesis duri

78 erturbed in a spo0J null mutant, we analysed replication initiation and origin positioning in (soj-sp

79 lays a key role in determining the timing of replication initiation and origin usage.

80 aA, DnaD, and DnaB to help properly regulate replication initiation and perhaps are analogous to the

81 he importance of protein acetylation for DNA replication initiation and progression, and suggests tha

82 of the response regulator CtrA represses DNA replication initiation and regulates the transcription o

83 se relationship between the durations of DNA replication initiation and replication.

84 h chloramphenicol relieves inhibition of DNA replication initiation and RNA synthesis in HipA-arreste

85 A genome-scale correlation between DNA replication initiation and RNA transcription, which is e

86 iscovery also adds to the connection between replication initiation and sister chromatid cohesion.

87 investigated the role of YabA in regulating replication initiation and the activity of DnaA as a tra

88 We demonstrate that SLD-2 is required for replication initiation and the nuclear retention of a cr

89 activate CtrA-dependent genes following DNA replication initiation and the reaccumulation of CtrA.

90 se checkpoint in mammalian cells to suppress replication initiation and to protect replication forks.

91 mitotic chromosomes but did not require its replication initiation and transactivation functions.

92 e DHFR locus, to comprehensively investigate replication initiation and transcription in this region.

93 dent pathway mediating the inhibition of DNA replication initiation, and a SpoT-independent pathway(s

94 r fundamental understanding of bacterial DNA replication initiation, and because of the conserved str

95 that confers stable episome maintenance, DNA replication initiation, and chromatin organization funct

96 f pre-replication complex (pre-RC) assembly, replication initiation, and micrococcal nuclease (MNase)

97 K is an S-phase-specific kinase required for replication initiation, and the DNA primase-polymerase i

98 We also found that DnaN activates replication initiation apparently through effects on Yab

99 ese results show that proteins implicated in replication initiation are selectively and differentiall

100 Perturbation of DNA replication initiation arrests human cells in G1, pointi

101 By picturing replication initiation as a two-state system and conside

102 tion of SirA enforces diploidy by inhibiting replication initiation as B. subtilis cells develop into

103 acetylation correlates with the frequency of replication initiation as measured by the abundance of s

104 mponent of the GINS complex required for DNA replication initiation, as a high-copy-number suppressor

105 r mechanism(s) of action at a distance, i.e. replication initiation at a considerable distance from t

106 h a model, suggesting that RepID facilitates replication initiation at a distinct group of human repl

107 , RecQ4, Treslin, and Drf1--are limiting for replication initiation at increasing N/C ratios in vitro

108 onsistent with models in which YabA inhibits replication initiation at oriC, and perhaps DnaA functio

109 ound that YabA function is mainly limited to replication initiation at oriC.

110 nations of chromosomal orc genes showed that replication initiation at oriC1 requires orc7 but not or

111 bility by providing a checkpoint that delays replication initiation at OriP.

112 icase loading, and is a pivotal event during replication initiation at replication origins.

113 iates diagnostic of canonical bi-directional replication initiation at the centrally located bi-direc

114 r by viral protein-driven multiple rounds of replication initiation at viral origins.

115 checkpoint is exerted by Chk1 not only upon replication initiation but also upon DNA elongation.

116 itutions of the 3 residues are functional in replication initiation but are immune to inhibition by S

117 ria regulate the frequency and timing of DNA replication initiation by controlling the activity of th

118 Soj is a dynamic protein that regulates DNA replication initiation by either inhibiting or activatin

119 Bacteria control the timing of replication initiation by regulating the activity of Dna

120 This suggests that defects in DNA replication initiation can lead to activation of the DNA

121 ost 35 kb downstream from these signals, and replication initiation can only be detected beyond this

122 By clearly defining the DNA replication initiation checkpoint in hypoxic cells, we n

123 n is dependent on polar remodeling and a DNA replication initiation checkpoint that is tightly integr

124 gin domain plays a role in the regulation of replication initiation, chromosome organization, and DNA

125 een E1 and E2, preventing pre-initiation and replication initiation complex formation.

126 loss of OBP function or OBP itself from the replication initiation complex.

127 promotes DNA unwinding and the assembly of a replication initiation complex.

128 prereplication complexes but were present in replication initiation complexes on ori-Lyt.

129 were observed for 11 and 15 on corresponding replication initiation complexes.

130 of transcription and the enhancement of DNA replication initiation, contains two GAGGC sequences arr

131 l oligomer in vivo and that the frequency of replication initiation correlates with the extent of Dna

132 the activity of different B-type cyclins in replication initiation correlates with the normal expres

133 ependent phenotype as the rid phenotype (for replication initiation defective).

134 Here we identify a replication-initiation determinant (RepID) protein that

135 Displaced Mcm2-7 double hexamers support DNA replication initiation distal to the loading site in vit

136 al states in response to both nucleotide and replication initiation/elongation factors.

137 FANCD2 to chromatin is strictly dependent on replication initiation, even in the presence of MMC indi

138 clease activity, DNA synthesis is slowed and replication initiation events are more frequent.

139 its associated E3s are rescued by increasing replication initiation events through mutating two maste

140 hosphorylation modulates the distribution of replication initiation events to insure genomic stabilit

141 of replication initiation sites showed that replication initiation events were absent from transcrip

142 s with high rates of transcription, very few replication initiation events were detected.

143 Moreover, very few replication initiation events were observed when two ect

144 me-wide massively parallel sequencing to map replication initiation events, thereby creating a databa

145 sequences strongly affected the location of replication initiation events, whereas histone modificat

146 ally required throughout the S-phase for all replication initiation events.

147 ather acts as a tunable rheostat to regulate replication initiation events.

148 progression while reducing the frequency of replication initiation events.

149 ine the spatial and temporal organization of replication initiation events.

150 ormal replication fork progression and fewer replication-initiation events.

151 expressed proteins identified, Cdc6 is a DNA replication initiation factor and exhibits oncogenic act

152 uggest that WDHD1 may also function as a DNA replication initiation factor as well as a G1 checkpoint

153 osis in fission yeast has identified the DNA replication initiation factor cdc18 (known as CDC6 in ot

154 phogenetic protein (BMP) pathway and the DNA replication initiation factor Cdc6.

155 es the DNA damage-induced degradation of the replication initiation factor Cdt1 via the CRL4(Cdt2) E3

156 Interestingly, the DNA replication initiation factor Cdt1, whose uncontrolled e

157 Geminin is known to inhibit the replication initiation factor Cdt1.

158 ecognition complex (ORC) is an essential DNA replication initiation factor conserved in all eukaryote

159 eplication by targeting the highly conserved replication initiation factor DnaA.

160 ta-TRCP)-dependent degradation of a limiting replication initiation factor Drf1.

161 The eukaryotic DNA replication initiation factor Mcm10 is essential for bot

162 We identified a mutation of the replication initiation factor Sld3, Sld3-m16, that is sp

163 And-1/Ctf4 is therefore a new replication initiation factor that brings together the M

164 ificial recruitment of a eukaryotic cellular replication initiation factor to a DNA sequence can crea

165 ation of cell division cycle 6 (Cdc6), a DNA replication initiation factor, by polo-like kinase 1 (Pl

166 ted by a region homologous to the yeast Sld2 replication initiation factor, followed by a cysteine-ri

167 The level of DnaA, a key bacterial DNA replication initiation factor, increases during the Caul

168 es, replication protein A encoding gene, DNA replication initiation factor, securin gene, nucleosome

169 inus of RECQ4 shares homology with yeast DNA replication initiation factor, Sld2, and is vital for em

170 ingly, pX increases expression of endogenous replication initiation factors Cdc6 and Cdtl while suppr

171 n fires is related to its ability to recruit replication initiation factors that are limiting within

172 on is a member of an expanding subset of DNA replication initiation factors that are not strictly req

173 By inhibiting replication initiation factors, we show that Cyclin E-in

174 of genetic material depends on essential DNA replication initiation factors.

175 The ability of Cdks to differentially effect replication initiation, factory activation, and progress

176 proteolysis is temporally restricted to late replication initiation following the requirements for DN

177 Bacillus subtilis DnaD is needed during replication initiation for assembly of the replicative h

178 LE may play a role in recombination-mediated replication initiation found in the mitochondria of mamm

179 Despite an increase in replication initiation frequency, cells lacking Mus81 us

180 e levels were generally associated with high replication initiation frequency.

181 and its kinase activity is required for DNA replication initiation from each origin.

182 Under such conditions, uncoupling of replication initiation from mitotic progression led to a

183 ed analysis of the sequence requirements for replication initiation from these replicators.

184 is essential for deoxyribonucleic acid (DNA) replication initiation from yeast to humans.

185 but rapidly adapted through mutations in the replication initiation gene trfA1.

186 s an important role in processes such as DNA replication initiation, gene expression regulation, and

187 A role in DNA replication initiation has been demonstrated and mapped

188 ly been shown to regulate DnaA-dependent DNA replication initiation; however, the mechanism underlyin

189 yabA encodes a negative regulator of replication initiation in Bacillus subtilis and homologu

190 YabA, a negative regulator of replication initiation in Bacillus subtilis, interacts w

191 Our results shed light on why replication initiation in bacteria is limited to a singl

192 regulatory mechanism for the control of DNA replication initiation in bacteria.

193 SeqA protein negatively regulates replication initiation in Escherichia coli and is also p

194 ew insight into the mechanisms that regulate replication initiation in Escherichia coli.

195 rved uncertainty on the position and time of replication initiation in eukaryote cell populations.

196 igin recognition complex (ORC) nucleates DNA replication initiation in eukaryotic cells.

197 se and show that MCM2 deficiency reduces DNA replication initiation in gene-rich regions of the genom

198 that the optimal cell cycle progression and replication initiation in M. tuberculosis requires that

199 gical origins and is valuable for studies on replication initiation in mammalian cells.

200 The genetic aspects of oriC replication initiation in Mycobacterium tuberculosis are

201 MRN at origin-proximal sites to control DNA replication initiation in response to DNA damage, thereb

202 and regulation are diverged features of DNA replication initiation in T. brucei, providing new insig

203 TDs to bind multiple partners to orchestrate replication initiation in the bacterial cell.

204 hromatin-binding studies show that E4 blocks replication initiation in vitro by preventing loading of

205 portant for downregulating CtrA prior to DNA replication initiation in vivo but that other phosphatas

206 During replication initiation in vivo, it is not known if these

207 Replication initiation is a key event in the cell cycle

208 to-stalked cell transition as usual, but DNA replication initiation is blocked.

209 an understanding of the complexities of how replication initiation is controlled and coordinated wit

210 and an origin-proximal promoter involved in replication initiation is delayed.

211 Control of DNA replication initiation is essential for bacterial cells

212 Control of DNA replication initiation is essential for cell growth.

213 Control of DNA replication initiation is essential for normal cell grow

214 ols the MBT and shows that the regulation of replication initiation is fundamental for normal embryog

215 g metazoan development and proposes that DNA replication initiation is governed differently in mitoti

216 Eukaryotic replication initiation is highly regulated and dynamic.

217 In summary, these findings indicate that replication initiation is influenced by transcription in

218 eplication fork damage, SeqA's repression of replication initiation is more important than its effect

219 th variability, invalidating the notion that replication initiation is the principal trigger for cell

220 me being circular, reinforcing the idea that replication initiation is triggered repeatedly by fork c

221 How Mcm10 is localized to sites of replication initiation is unclear, as current models ind

222 kinase and CDK targets at origins regulates replication initiation kinetics during S-phase and estab

223 Our data suggest that increased replication initiation leads to slow replication fork pr

224 We purified components of the replication initiation machinery and studied their inter

225 modelling have yielded detailed genome-wide replication initiation maps.

226 gin positioning, chromosome partitioning and replication initiation may be a secondary consequence of

227 Replication initiation must be a carefully regulated pro

228 ate that elevated levels of helicase perturb replication initiation not only at origins of replicatio

229 of Hda is tightly controlled to ensure that replication initiation occurs only once per cell cycle.

230 lays a key role during the transcription and replication initiation of vRNA genome.

231 but these were not directly associated with replication initiation or ongoing chromosome replication

232 Conditions that perturb replication initiation or replication elongation induce

233 ular processes of transcription, DNA repair, replication initiation, or polymerase elongation and the

234 m DNA molecules, as well as in bidirectional replication initiation outside of the main replication o

235 Replication initiation peaks were AT rich, and >50% of t

236 riments, and we discovered that cell size at replication initiation per origin, namely the initiation

237 For replication initiation, plasmid P1 requires three origin

238 initiation sites at oriC1 were determined by replication initiation point analysis and found to map d

239 eling and histone deacetylation with the DNA replication initiation process at OriP.

240 ion of the SaPI replication origin (ori) and replication initiation protein (Rep), which has helicase

241 gin involves interactions of the RK2 plasmid replication initiation protein (TrfA) with both the poly

242 lated directly by DnaA, the highly conserved replication initiation protein and transcription factor.

243 a a transcriptional response mediated by the replication initiation protein DnaA.

244 lly occurring plasmid that does not encode a replication initiation protein or a partitioning system

245 Both the replication initiation protein ORC and telomeres or a li

246 This result suggests that loss of a replication initiation protein prevents progression of t

247 Here we show that the replication initiation protein Sld3 is phosphorylated by

248 the ability of Cdk2 to phosphorylate the key replication initiation protein treslin in vitro Furtherm

249 hough they do not resemble any characterized replication initiation protein.

250 Nicking is performed by a replication-initiation protein (Rep) that directly binds

251 lication caused by overexpression of various replication initiation proteins such as Dpb11 and Sld2.

252 ies of hypomorphic alleles of genes encoding replication initiation proteins suggest the question of

253 Replication initiation proteins, known as prereplicative

254 t-range IncP-1 plasmids generally encode two replication initiation proteins, TrfA1 and TrfA2.

255 DnaD and DnaB are essential DNA-replication-initiation proteins in low-G+C content Gram-

256 Lowering the DNA replication initiation rate by introducing the dnaA(Sx)

257 six essential ATPases whose contributions to replication initiation remain unclear.

258 wever, how the CDK cycle is coordinated with replication initiation remains elusive.

259 Plasmid encoded replication initiation (Rep) proteins recruit host helic

260 into three measurable variables representing replication initiation, replication-division cycle, and

261 ication elongation, mutants defective in DNA replication initiation require the Chk1 kinase.

262 Template unwinding during DNA replication initiation requires the loading of the MCM h

263 mplexes in a manner that exposes a potential replication initiation site.

264 ries of restriction fragments that contained replication initiation sites (bubbles) in vivo.

265 nct DNA-protein interactions at subgroups of replication initiation sites (replication origins) modul

266 ntiate TNR instability; therefore, we mapped replication initiation sites and prereplication complex

267 findings indicate that Mcm10 is localized to replication initiation sites by directly binding MCM thr

268 by HU suggests that there are many potential replication initiation sites in permissive parts of the

269 Mapping of replication initiation sites in the HAC revealed that re

270 High-resolution mapping of replication initiation sites showed that replication ini

271 Data presented here identify replication initiation sites throughout the genome, prov

272 n events, thereby creating a database of all replication initiation sites within nonrepetitive DNA in

273 Orc2 and Mcm4 bound near each of the replication initiation sites, but a dramatic change in (

274 define a new mechanism for targeting ORC to replication initiation sites.

275 s, resulting in a corresponding shift in DNA replication initiation sites.

276 inant (RepID) protein that binds a subset of replication-initiation sites.

277 These data suggest a conserved mechanism for replication initiation: Sld3/Treslin coordinates Cdc45 r

278 rapid cell lethality, disrupts the timing of replication initiation, slows replication elongation, an

279 ry - the factors that demarcate sites of the replication initiation, termed origins - are diverged fr

280 Finally, we show that the changes in DNA replication initiation that take place during B cell dev

281 This is due to the absence of replication initiation that would typically occur upstre

282 During replication initiation, the core component of the helica

283 Shortly after replication initiation, the duplicated origins move as a

284 After replication initiation, the two bidirectionally oriented

285 cation resumed from oriC after inhibition of replication initiation, these high levels of binding dec

286 sents a repressive compartment that inhibits replication initiation, thus maintaining the boundaries

287 We see no long-lived replication "factory"; replication initiation timing does not covary with cell

288 nctionally distinct Rpd3 complexes to affect replication initiation timing supports the idea that his

289 stone hyperacetylation and delocalization of replication initiation, trichostatin A treatment of cell

290 hat cell division licenses the next round of replication initiation via these changes.

291 n gene expression, separate from its role in replication initiation, we analyzed changes in mRNA leve

292 vitro assay for eukaryotic origin-dependent replication initiation, we investigated the control of t

293 rereplicative complex (pre-RC) formation and replication initiation when fused to the GAL4 DNA-bindin

294 t explain how monomers outcompete dimers for replication initiation when plasmid copy number and pi s

295 propose a model for Sld3-mediated control of replication initiation, wherein Sld3 manages the proper

296 me duplication is controlled at the level of replication initiation, which occurs at cis-acting repli

297 ribe the reconstitution of budding yeast DNA replication initiation with 16 purified replication fact

298 We show that DnaA coordinates DNA replication initiation with cell cycle progression by ac

299 We propose that DnaA couples DNA replication initiation with the expression of the two os

300 ee extracts we established a system to study replication initiation within linear telomeric DNA subst