ライフサイエンスコーパス: replication origin

1 crete sites in the genome, termed origins of replication (origins).

2 III (Pol III) holoenzyme association at the replication origin.

3 The SNP maps directly within the replication origin.

4 m that of the herpes simplex virus (HSV) DNA replication origin.

5 loaded at centromeric sites adjacent to the replication origin.

6 head-to-head hexameric helicases around the replication origin.

7 e host cell DNA replication machinery to the replication origin.

8 forks thereby suppressing the firing of new replication origins.

9 the local chromatin environment surrounding replication origins.

10 ncomitant increase in the utilization of new replication origins.

11 cytoplasm of VACV-infected cells to pinpoint replication origins.

12 tion, and decreased association of GINS with replication origins.

13 to enhanced loading of Cdc45 onto potential replication origins.

14 lication without activating a novel group of replication origins.

15 ate and severe defect in the partitioning of replication origins.

16 de insight into the nature of eukaryotic DNA replication origins.

17 nt response to inhibit further firing of DNA replication origins.

18 s from p53-/- mice and inhibit firing of DNA replication origins.

19 ng RNAs, promoters, regulatory sequences and replication origins.

20 otic DNA replication initiates from multiple replication origins.

21 d imply that the repetitive monomers contain replication origins.

22 tic genomes are replicated from multiple DNA replication origins.

23 ly occurs within a nucleosome-free region at replication origins.

24 in the association of DDK with MCM rings at replication origins.

25 cation forks while suppressing firing of new replication origins.

26 defects, along with diminished RPA signal at replication origins.

27 he DDK- and CDK-dependent activation of late replication origins.

28 vation of the Cdc45-MCM-GINS helicase at DNA replication origins.

29 d the Mcm2-7 replicative helicase complex at replication origins.

30 icensing, which entails loading MCM-2-7 onto replication origins.

31 votal event during replication initiation at replication origins.

32 lex interplay of many regulatory proteins at replication origins.

33 and may be a feature found in all bacterial replication origins.

34 pon relief of stress, HBO1 reassociates with replication origins.

35 proteins recognize specific sequences within replication origins.

36 recognition complex proteins and function as replication origins.

37 hese proteins localize to the DUEs of active replication origins.

38 ient number of appropriately distributed DNA replication origins.

39 ading of the MCM helicase activator Cdc45 at replication origins.

40 egetative cells, despite their lack of known replication origins.

41 e in the Cdk2-mediated loading of Cdc45 onto replication origins.

42 h FACT and the replicative helicase MCM with replication origins.

43 eplication starts at initiation sites termed replication origins.

44 ) proteins recruit host helicases to plasmid replication origins.

45 ss at site-specific stalled forks present at replication origins.

46 c positions on chromosomes that serve as DNA replication origins.

47 have a single-circular chromosome with three replication origins.

48 DNA replication proteins over the number of replication origins.

49 eplicative helicase, into double hexamers at replication origins.

50 the genome-wide identification of human DNA replication origins.

51 oes so in close proximity to the pre-defined replication origins.

52 dentification of only a handful of mammalian replication origins.

53 d a 26-bp sequence related to alphasatellite replication origins.

54 tion initiation at a distinct group of human replication origins.

55 tivation of MCM helicase complexes loaded at replication origins.

56 tes on loaded Mcm double hexamers at unfired replication origins [9, 10].

57 e to the dynamic selection and activation of replication origins across diverse cell types and develo

58 double hexamers and during S phase licensed replication origins activate to initiate bidirectional r

59 icate that NCOA4 acts as an inhibitor of DNA replication origin activation by regulating CMG (CDC45/M

60 lished the succession of events that lead to replication origin activation by the MCM and recent stud

61 Cdc7 kinase is required for replication origin activation, is a target of the intra-

62 sion, while preserving the relative order of replication origin activation.

63 DNA replication origin activity changes during development.

64 ression during normal S phase by controlling replication origin activity.

65 of the prereplicative complex at chromosomal replication origins after exposure to UV light (UVC).

66 ssentially conferred by TrfA2 and the intact replication origin alone but that TrfA1 is nonetheless i

67 DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuri

68 lthough G4-forming sequences are abundant in replication origins, an asymmetry in nucleotide distribu

69 tenance (MCM) helicase complexes at many DNA replication origins, an essential process termed origin

70 ntegration of cassettes containing the c-myc replication origin and (CTG)(n) . (CAG)(n) TNRs in HeLa

71 ents for anchoring polymerase at the plasmid replication origin and bring insights of how the directi

72 both Orc1 and Cdc6 functions by binding to a replication origin and directly recruiting MCM helicase.

73 ld-type SUV3 associated with an active mtDNA replication origin and facilitated mtDNA replication, wh

74 e chromatin, does not depend on the episomal replication origin and initiates at multiple single-stra

75 e chromosome arms lie side-by-side, with the replication origin and terminus at opposite cell poles.

76 rc2-S188 phosphorylation associates with DNA replication origin and that cells expressing Orc2-S188A

77 Interestingly, premutation hESCs have a replication origin and the T variant similar to nonaffec

78 DNA replicative helicase, has fewer dormant replication origins and an increased number of stalled r

79 e, through a comparative genomic analysis of replication origins and chromosomal replication patterns

80 ing of the MCM complex onto chromatin at the replication origins and decreased cyclin D1 levels, wher

81 ctions in the regulation of the licensing of replication origins and expanding the scope of its overa

82 T530-pSIRT1 associates with replication origins and inhibits replication from a grou

83 atin, methylates histone H3 lysine 4 at late replication origins and inhibits the loading of CDC45 to

84 ryotic DNA synthesis initiates from multiple replication origins and progresses through bidirectional

85 tem cells to regulate the temporal firing of replication origins and quality control of replicated DN

86 We used natural budding yeast DNA replication origins and synthetic DNA sequences to show

87 and repair, including the identification of replication origins and termination regions.

88 rrelate with sequence motifs associated with replication origins and with locations that are preferen

89 H4K20me2 is enriched at replication origins, and abrogating ORC1 recognition of

90 ne expression patterns, specification of DNA replication origins, and definition of chromatin domains

91 , cohesin is transiently recruited to active replication origins, and it spreads along DNA as forks p

92 ents induce localized fork stalling at yeast replication origins, and that localized stalling is depe

93 ion events at a G-quadruplex region near the replication origin are thought to drive replication of m

94 During late mitosis and G1 replication origins are "licensed" by loading MCM2-7 dou

95 Replication origins are cis-acting elements that potenti

96 Transcription and DNA replication origins are correlated, but the association

97 Eukaryotic replication origins are defined by the ORC-dependent loa

98 complete genome replication is maximized if replication origins are evenly spaced, the largest inter

99 Collectively, our data indicate that replication origins are fundamental organizers and regul

100 During the gap between G1 and S phases when replication origins are licensed and fired, it is possib

101 Replication origins are licensed by loading MCM2-7 hexam

102 high resolution in C. elegans, we show that replication origins are marked with specific histone mod

103 DNA replication origins are necessary for the duplication of

104 ntrary to the bacterial paradigm, eukaryotic replication origins are not irrevocably defined by selec

105 sis I is distinct from mitotic exit, in that replication origins are not licensed by Mcm2-7 chromatin

106 control the location and timing of firing of replication origins are poorly understood.

107 In metazoans, how replication origins are specified and subsequently activ

108 Contrary to expectation, we find that replication origins are specified prior to the broad ons

109 Replication origins are under tight regulation to ensure

110 d by about 3-fold, suggesting that fewer DNA replication origins are used in E1A-expressing cells.

111 DNA circles (GAP1(circle)) contain GAP1, the replication origin ARS1116, and a single hybrid LTR deri

112 The complex loads at replication origins as an inactive double-hexamer encirc

113 cy, cells lacking Mus81 use the same pool of replication origins as Mus81-expressing cells.

114 ssDNA revealed intergenic regions, including replication origins, as hot spots for replication stress

115 BAH domain contributes to ORC's selection of replication origins, as well as new tools for examining

116 gin recognition complex (ORC) to establish a replication origin at one element of oriP, DS (dyad symm

117 scopic and biochemical studies implicating a replication origin at the end of the VACV genome.

118 K293) cells, we identified the HBoV1 minimal replication origin at the right-end hairpin (OriR).

119 By mapping replication origins at different developmental stages, w

120 Re-replication occurs from specific replication origins at six genomic loci, termed Drosophi

121 at lysine 4, known to prevent firing of late replication origins at the early S phase.

122 al approaches to identify start sites of DNA replication (origins) based on the presence of defining

123 he nucleotide-binding domain of NLRs and DNA replication origin-binding Cdc6/Orc1 proteins.

124 m2-7 complexes assemble and are recruited to replication origins, but are defective in helicase loadi

125 hly regulated process that is initiated from replication origins, but the elements of chromatin struc

126 yotes duplicate their genomes using multiple replication origins, but the organization of origin firi

127 isplacement assays, indicate that DnaA opens replication origins by a direct ATP-dependent stretching

128 of active CMG (Cdc45-MCM-GINS) helicases at replication origins by a set of conserved and essential

129 Here, we show that LMO2 is recruited to DNA replication origins by interaction with three essential

130 The current model suggests that replication origins compete for a limited pool of initia

131 undaries separate regions of similarly timed replication origins connecting the long-known effect of

132 erevisiae (38%), and contains abundant yeast replication origin consensus sites (ACS) evenly distribu

133 el wherein GINS trades places with Sld3 at a replication origin, contributing to the activation of th

134 ion terminates when replisomes from adjacent replication origins converge.

135 ement (DUE)-binding protein (DUE-B) binds to replication origins coordinately with the minichromosome

136 results suggest that a T/C SNP located at a replication origin could contribute to the inactivation

137 Whether Mcm10 recruitment to replication origins depends on CMG helicase assembly has

138 ication proteins, and its recruitment to DNA replication origins depends on the two pre-replicative c

139 egration of small replication bubbles causes replication origin destruction.

140 Cellular DNA replication origins direct the recruitment of replicativ

141 pear to regulate replication time by binding replication origins directly, nor is its effect on telom

142 Eukaryotic cells license each DNA replication origin during G1 phase by assembling a prere

143 lin-dependent kinases, known to activate DNA replication origins during firing, inhibits MDM2-mediate

144 ome maintenance (MCM2-7) helicase complex at replication origins during G1 phase as an inactive doubl

145 maintenance (MCM) complex is first loaded at replication origins during G1 phase, and then converted

146 in recognition complexes (ORCs) that bind to replication origins during most of the cell cycle and di

147 Inserting at unfired replication origins during S phase may allow P elements

148 d in part by sequestration of DDK at unfired replication origins during S phase.

149 Sld3 and Cdc45 bind to early replication origins during the G(1) phase of the cell cy

150 re we identify a new indispensable bacterial replication origin element composed of a repeating trinu

151 ) into pre-replicative complexes at multiple replication origins ensures precise once per cell cycle

152 rtQTLs involved the differential use of replication origins, exhibited allele-specific effects o

153 fission yeast and shows that in human cells replication origins fire stochastically forming clusters

154 isplay increased sister chromatid exchanges, replication origin firing and chromosomal aberrations.

155 se complexes, required for initiation of DNA replication origin firing and ongoing DNA synthesis duri

156 s program is based on the regulation of both replication origin firing and replication fork progressi

157 pha regulatory subunit, becomes limiting for replication origin firing at high N/C ratio, and this in

158 tant DNA synthesis was due to an increase in replication origin firing that compensated for reduced f

159 of the spatial and temporal organization of replication origin firing, analyzed using single DNA mol

160 cellular processes, including suppression of replication origin firing, promotion of deoxynucleotide

161 It controls and coordinates DNA-replication origin firing, replication-fork stability, c

162 We have shown that GOF p53 increases DNA replication origin firing, stabilizes replication forks,

163 cation timing is determined by the timing of replication origin firing, which involves activation of

164 of DNA replication fork slowing and reduced replication origin firing.

165 se replication elongation checkpoint and the replication origin-firing checkpoint induced by camptoth

166 Examples include replication origin firings, formation of chromatin loops

167 MCM complexes are recruited to replication origins for genome duplication.

168 the nucleus through epigenetic regulation of replication origin function.

169 sequencing (NS-seq) is used to discover DNA replication origins genome-wide, allowing identification

170 ifferential replication timing of eukaryotic replication origins has long been linked with epigenetic

171 D-loop replication origins have been mapped in several species.

172 sms that control the spatial organization of replication origins have potential impacts for genome re

173 id replication does not require a functional replication origin; however, in the presence of competit

174 ssion is associated with increased firing of replication origins, impaired replication fork progressi

175 could contribute to the inactivation of this replication origin in FXS hESCs, leading to altered repl

176 the unwound DNA within the Escherichia coli replication origin in the helicase loading process, but

177 on in Escherichia coli cells with an ectopic replication origin in which highly transcribed rrn opero

178 tiates at multiple discrete regions known as replication origins in a dynamic yet regulated manner to

179 mosomes initiate DNA synthesis from multiple replication origins in a temporally specific manner duri

180 bodies, epigenetic regulatory elements, and replication origins in Drosophila cells.

181 Different replication origins in eukaryotes are activated at diffe

182 understanding the mechanisms that define DNA replication origins in eukaryotes.

183 s required for the clustering of a subset of replication origins in G1 phase and for the early initia

184 n is pivotal for the proper selection of DNA replication origins in higher eukaryotes.

185 ATR and Cdc7 kinase-dependent mechanisms at replication origins in human cells.

186 ate the assembly of MCM2-7 onto chromatin at replication origins in late mitosis and G(1) phase.

187 Conversely, the weakening of replication origins in repetitive regions suppresses the

188 ation speed along with activation of dormant replication origins in response to SAHA.

189 , our findings support the existence of more replication origins in T. brucei than previously appreci

190 ion forks emerging from any one of the three replication origins in the Sulfolobus chromosome remain

191 rted to be essential for loading MCM2-7 onto replication origins in the Xenopus oocyte extract system

192 Fkh1-dsm binds replication origins in vivo but fails to cluster them, s

193 We previously showed how the distribution of replication origins in yeasts promotes complete genome r

194 itiation of DNA replication from RepID-bound replication origins, including the origin at the human b

195 n that emerges from the stochastic nature of replication origins initiation.

196 rectional loading of two ring helicases at a replication origin is achieved by strictly regulated and

197 whereas accumulating evidence indicates the replication origin is also affected.

198 The pUC replication origin is controlled by a genetic switch for

199 o assembly of the replication complex at the replication origin is, or how the directionality of repl

200 osome maintenance proteins 2-7 (MCM2-7) onto replication origins is a prerequisite for replication fo

201 g of the Origin Recognition Complex (ORC) to replication origins is essential for initiation of DNA r

202 The specification of mammalian chromosomal replication origins is incompletely understood.

203 of the temporally coordinated activation of replication origins is the establishment of broad domain

204 A key feature of replication origins is their ability to control the onse

205 ure rates that are consistent with data from replication origin knockout experiments.

206 The separation of DNA replication origin licensing and activation in the cell

207 7 genes, which is expected to compromise DNA replication origin licensing and result in elevated rate

208 This is believed to be achieved by limiting replication origin licensing and thereby restricting the

209 s as head-to-head double hexamers during DNA replication origin licensing is crucial for ensuring onc

210 product levels and proteins involved in DNA replication origin licensing may explain the deleterious

211 CDC6 is essential for eukaryotic replication origin licensing, however, it is poorly unde

212 Knockdown of the replication origin-licensing factor CDT1 eliminates both

213 explain how specific proteins recognize DNA replication origins, load the replicative helicase on DN

214 ed to repeat expansion in haplogroup D and a replication origin located approximately 53 kb upstream

215 Importantly, we found a replication origin located near an ORC1/CDC6 binding sit

216 zoan replication timing program, clusters of replication origins located in different subchromosomal

217 e origin recognition complex (ORC) specifies replication origin location.

218 t subgroups of replication initiation sites (replication origins) modulate the ubiquitous replication

219 yeasts limits the availability of efficient replication origin modules to only a handful of species

220 However, different approaches to mapping replication origins, namely (i) sequencing isolated smal

221 Here we show that the replication origin of an Agrobacterium tumefaciens Ti pl

222 tion of a lacZalpha cassette proximal to the replication origin of the phage used to construct the li

223 The replication origin of Vibrio cholerae chromosome II (chr

224 bility of specific recognition motifs within replication origins of higher organisms.

225 ls, involving the assembly and activation at replication origins of the CMG (Cdc45-MCM-GINS) DNA heli

226 Helicase loading at a DNA replication origin often requires the dynamic interactio

227 accumulating especially at the heavy strand replication origin OH, in the ribosomal genes (12S and 1

228 l replication initiation outside of the main replication origin, OH.

229 genes (12S and 16S) and at the light strand replication origin OL.

230 ains, that in vitro stretches DNA to promote replication origin opening.

231 se regions are characterized by a paucity of replication origins or unusual DNA structures.

232 ced by loss of licensing control at cellular replication origins, or by viral protein-driven multiple

233 H4K20me2 in cells impairs ORC1 occupancy at replication origins, ORC chromatin loading and cell-cycl

234 ation-dependent replication at mitochondrial replication origin ori5 in hypersuppressive rho- cells.

235 in DnaA and its interactions with the unique replication origin oriC.

236 DnaA monomers recognize the replication origin (oriC) by binding double-stranded DNA

237 replichores) on opposite cell halves and the replication origin (oriC) close to midcell.

238 fforts focused on recombination proteins and replication origin (oriC) degradation.

239 , interactions of DnaA-ATP monomers with the replication origin, oriC, must be carefully regulated du

240 We found that in new offspring, chromosome replication origins (oriCs) are arranged in a three-dime

241 At replication origins, our data revealed asymmetric locali

242 3681 lysis vector derivatives with different replication origins (pBR, p15A, pSC101), resulting in pY

243 terodimer with a free TP that recognizes the replication origins, placed at both 5' ends of the linea

244 umulating evidence suggests that dormant DNA replication origins play an important role in the recove

245 to recruit a single Cdt1-Mcm2-7 heptamer to replication origins prior to Cdt1 release and ORC-Cdc6-M

246 s promoted by HBO1 acetylating histone H4 at replication origins, providing a molecular view of how c

247 meres and chromosomal arm regions containing replication origins proximal to binding sites for Taz1,

248 esponsible polymerase, replication time, and replication origin proximity.

249 To investigate the properties of metazoan replication origins, recent studies in cell culture have

250 are normally associated with the chromosome replication origin region (ori).

251 plication and the nature and location of the replication origins remain unknown.

252 ID is involved in an interaction between the replication origin (Rep-P) and the locus control region.

253 We suggest that replication origins, replication timing, and replication

254 idirectional replication from eukaryotic DNA replication origins requires the loading of two ring-sha

255 ted at particular sites on the genome called replication origins (ROs).

256 ble-stranded oligonucleotides containing the replication origin sequence without the parental TP.

257 Minimal replication origin sequences used as probes identified t

258 This raises the question of what purpose replication origins serve and why they have evolved.

259 tic mechanisms are important for determining replication origin sites in budding yeast, highlighting

260 ing studies, and to include the collation of replication origin sites in the fission yeast Schizosacc

261 e mapping studies of confirmed and predicted replication origin sites.

262 cation initiator proteins to pericentromeric replication origins so that they initiate replication ea

263 tion initiates from defined locations called replication origins; some origins are highly active, whe

264 east, highlighting mechanistic principles of replication origin specification that are common among e

265 We show that chromatin enforces DNA replication origin specificity by preventing non-specifi

266 assay has been a valuable tool in dissecting replication origin structure and function.

267 ute to the function and relative strength of replication origins, suggesting that the chromatin envir

268 d Lachancea waltii, we assess to what extent replication origins survived genomic change produced fro

269 e majority of the ORCA-bound sites represent replication origins that also associate with the repress

270 ulin heavy chain (Igh) locus, which contains replication origins that are silent in embryonic stem ce

271 tin islands at regions corresponding to late replication origins that are sites of double-strand brea

272 contained consensus sequences for autonomous replication origins that could explain their maintenance

273 dicate that NCOA4 acts as a regulator of DNA replication origins that helps prevent inappropriate DNA

274 genetic material spreading outward from the replication origin: the extent of TLD correlates with th

275 , leading to rapid dissociation of HBO1 from replication origins, thereby blocking initiation of DNA

276 o the identification of tens of thousands of replication origins throughout mammalian genomes, provid

277 Bioinformatic analysis detects DnaA-trios in replication origins throughout the bacterial kingdom, in

278 actions during homologous recombination, and replication origin timing and long-range origin clusteri

279 s transcription factors yet are defective in replication origin timing control.

280 s, Fkh1 and Fkh2, are global determinants of replication origin timing.

281 g and thereby restricting the firing of each replication origin to once per cell cycle.

282 origin firing that determine the ability of replication origins to accrue limiting factors and have

283 interactions between initiator proteins and replication origins to assemble a pre-replicative comple

284 ose a hypothesis based on aberrant firing of replication origins to explain intragenic nonrecurrent r

285 romosome structure regulates the capacity of replication origins to initiate, very little is known ab

286 th various cellular activities) domains bind replication origins to license new rounds of DNA synthes

287 plasmid-based expression systems require DNA replication origins to maintain plasmids efficiently.

288 orm higher-order oligomers that process host replication origins to promote replisome formation.

289 al agent of sleeping sickness, localized its replication origins to the boundaries of multigenic tran

290 Bacteria use the replication origin-to-terminus polarity of their circula

291 ur upstream of FMR1, suggesting that altered replication origin usage combined with fork stalling pro

292 ly minor perturbations to primary or dormant replication origin usage contribute to accelerated genet

293 hted by the finding that the distribution of replication origins varies between differentiated cell t

294 of the HBO1 coactivator to target genes and replication origins via JNK-mediated phosphorylation of

295 ypersensitivity to licensing inhibition when replication origins were removed.

296 ruits the MCM2-7 replicative helicase to the replication origin, where MCM2-7 is activated to initiat

297 We localized the minimal replication origin, which contains both NS1 nicking and

298 rect observation of stochastic firing of DNA replication origins, which differs from cell to cell.

299 of mammalian genomes starts at sites termed replication origins, which historically have been diffic

300 lication from a group of 'dormant' potential replication origins, which initiate replication only whe