ライフサイエンスコーパス: replicon

1 ded for RNA genome replication (intragenomic replicons).

2 mic acids for inhibition of a HCV subgenomic replicon.

3 P) reporter-expressing West Nile virus (WNV) replicon.

4 n sRNA and the name of a sequenced bacterial replicon.

5 d contains a stably replicating HCV reporter replicon.

6 lation-deficient, replication-defective JFH1 replicon.

7 ts too large to be accounted for by a single replicon.

8 cells, which stably express an HCV subgenome replicon.

9 was examined in the context of a subgenomic replicon.

10 from cells that harbor an HCV subgenomic RNA replicon.

11 tumors initiated by parent cells lacking the replicon.

12 y decreases replication of an HEV subgenomic replicon.

13 ory effect of cGAMP than was that of 1b/Con1 replicon.

14 ectious virus and a corresponding subgenomic replicon.

15 uelan equine encephalitis virus (VEEV)-based replicons.

16 says using western equine encephalitis virus replicons.

17 icromolar potency against both G-1a and G-1b replicons.

18 ies have been achieved using DNA virus-based replicons.

19 pic screening campaigns using HCV subgenomic replicons.

20 rational means to inhibit these tumorigenic replicons.

21 linical polymorphic and resistant HCV mutant replicons.

22 tain stable, genotype 4a luciferase-encoding replicons.

23 ular level utilizing hepatitis C virus (HCV) replicons.

24 Cas9 when they are delivered on geminivirus replicons.

25 gmented and predominantly composed of linear replicons.

26 023 inhibited replication of subgenomic HCV replicons.

27 ssible mechanisms for local melting in other replicons.

28 anisms similar to those found in other ssDNA replicons.

29 by using genotype 4 NS5A hybrid genotype 2a replicons.

30 in antigens are encoded by encapsulated mRNA replicons.

31 ith a focus on the role of each of its three replicons.

32 lex entities that process multiple clustered replicons.

33 ded among two or more large chromosome-sized replicons.

34 chromatin context at the level of individual replicons.

35 -associated translation activity in the RNA2 replicons.

36 tures or after electroporation of selectable replicons.

37 Using chimeric replicons, 2a NS4B was identified to confer resistance t

38 rld include those expected for primitive RNA replicons: (a) small size imposed by error-prone replica

39 In wheat cells, the replicons achieve a 110-fold increase in expression of a

40 Although the replicon activity of the new agents containing pyrimidin

41 with 99% or more of the cells retaining the replicon after each cell division.

42 o be >99% based on results from in vitro HCV replicon amplification, with the same extrapolated amoun

43 ll line that contained an HCV subgenomic RNA replicon and also expressed a GFP-LC3 fusion protein.

44 rom cells harboring a replicating subgenomic replicon and analyzed the purified protein by mass spect

45 Both the WEEV replicon and combination V/W/E vaccination, however, eli

46 ay analysis of flies harboring an alphavirus replicon and control green fluorescent protein flies ide

47 double-stranded RNA (dsRNA) or an FHV-based replicon and facilitates the natural infection of C. ele

48 ectedly, replication of the genotype 2a JFH1 replicon and infectious JFH1 virus was less sensitive to

49 licon but was less effective for the 2a/JFH1 replicon and infectious JFH1 virus.

50 ibitors (compound 17) was active against the replicon and inhibited the helicase more than 50% at 2.6

51 l of CHIKV infection and showed that the DNA replicon and protein antigen were potent vaccine candida

52 ibe the development and testing of novel DNA replicon and protein CHIKV vaccine candidates and evalua

53 Using a PV replicon and purified virion RNA, we also show that eIF4

54 , finds fragments of those tDNAs in the same replicon and removes unlikely candidate islands through

55 zed and transcribed a genotype 4a subgenomic replicon and transfected Huh7-Lunet cells with it, which

56 Similarly, the EEEV replicon and V/W/E combination vaccine elicited neutrali

57 Resistance selection studies with the JFH1 replicon and virus systems identified drug-induced mutat

58 ication of infectious SARS-CoV as well as of replicons and human coronavirus NL63.

59 TNF had little effect on HCV replicons and increased entry of HCV pseudoparticles.

60 duction, and cell entry were monitored using replicons and infectious HCV.

61 ctivities using genotype 2a JFH-1 subgenomic replicons and infectious virus systems.

62 vitro potency against wild-type hepatitis C replicons and known clinical polymorphic and resistant H

63 tic insertion of this tag resulted in viable replicons and specific labelling while preserving the ef

64 y leading to the establishment of subgenomic replicons and the infectious virus model (HCVcc).

65 456 inhibits an artificial Hepatitis C viral replicon, and has broad antifungal activity, suggesting

66 radation in cells infected with the virus or replicons, and analyzed autophagosome contents by confoc

67 n be formed with multiple antigen-expressing replicons, and is capable of eliciting both CD8(+) T-cel

68 However, it remains unknown how replicons are organized for processing at each replicati

69 havirus genome-based, self-replicating RNAs (replicons) are widely used vectors for expression of het

70 in 10-fold improvement in potency in a WEEV replicon assay and up to 40-fold increases in half-lives

71 -1 benzyl groups which achieved breakthrough replicon assay potency (EC(50) = 1 nM).

72 bited the most promising activity in the RSV replicon assay with an EC50 of 0.15 muM.

73 ve in the hepatitis C virus (HCV) cell-based replicon assay, which was corroborated on examination of

74 -5172 activity was determined using a mutant replicon assay.

75 ovis and also against hepatitis C virus in a replicon assay.

76 iviral activity in a dengue virus subgenomic replicon assay.

77 in the N-terminal alpha helix, and packaged replicon assays showed that rescue could be mediated by

78 d by 12, were found to be more potent in HCV replicon assays than leading second generation inhibitor

79 Reporter virus and replicon assays using phosphorylation-ablated alanine mu

80 type that established a potency benchmark in replicon assays, particularly toward HCV GT-1a, a strain

81 Using Northern blot and reporter replicon assays, we demonstrated that both small molecul

82 pounds showing submicromolar activity in HCV replicon assays.

83 compared with a reference strain in in vitro replicon assays.

84 revir and daclatasvir was assessed using HCV replicon assays.

85 echanism by which RepA mediates the specific replicon assembly of staphylococcal multiresistant plasm

86 at 2 +/- 1 muM, inhibited the subgenomic HCV replicon at 10 muM, and was not toxic at 100 muM.

87 In tobacco (Nicotiana tabacum), replicons based on the bean yellow dwarf virus enhanced

88 ncy against the NS3 protease in a subgenomic replicon-based cellular assay (Huh-7).

89 stress generated during HCV replication in a replicon-based model also induced STAT3 activation.

90 In the present work, we developed a replicon-based system for genome engineering of cereal c

91 e mathematical model showing how neighboring replicons became associated stochastically to form repli

92 We recently reported on intragenomic replicons, bicistronic viral transcripts expressing an a

93 ibit the replication of HCV genotype 1b Con1 replicon but was less effective for the 2a/JFH1 replicon

94 priming sites of the DNA primase of the pRN1 replicon, but nearly all these mutations created nonsens

95 uced into either ORF within the intragenomic replicon, but unlike many other mutations required the o

96 sion of double-stranded DNA from an episomal replicon by CRISPR/Cas9, coupled to lambda-red-mediated

97 rmined the relative frequency of preexisting replicons capable of establishing foci during treatment

98 Furthermore, replicons carrying CRISPR/Cas9 nucleases and repair temp

99 antitatively measured using HCV infected and replicon cell culture.

100 viral activities against CHIKV using a CHIKV replicon cell line and clinical isolate of CHIKV of Cent

101 ng from 0.2 to >98 muM, measured in the Huh7-replicon cell line, with no apparent cytotoxicity (CC50

102 BMS-790052 inhibits genotype 2a JFH1 replicon cells and cell culture infectious virus with 50

103 expression in HCV-infected hepatocytes using replicon cells containing full-length HCV genotype 1 and

104 ed that the autophagosomes purified from HCV replicon cells could mediate HCV RNA replication in a li

105 ith genotype 1a full-length (FL) HCV genomic replicon cells or genotype 2a Japanese fulminant hepatit

106 The addition of cGAMP into 1b/Con1 replicon cells significantly increased the expression of

107 amma when PBMC were cocultured with Huh7/HCV replicon cells than with Huh7 cells; NK cells and PBMCs

108 Replicon cells were labeled with virus-specific peptides

109 se-tagged subgenomic HCV replicons (Huh7/HCV replicon cells) or their HCV-negative counterparts (Huh7

110 mpounds showing (i) nanomolar potency in HCV replicon cells, (ii) limited toxicity and off-target act

111 icacy in a triple combination regimen in HCV replicon cells, and exhibited consistently high oral bio

112 educed expression of fibrotic markers in HCV replicon cells.

113 ed autophagy of lipids in virus-infected and replicon cells.

114 studied in NOD/SCID mice engrafted with HCV replicon cells.

115 tween IRF5- and empty vector-transfected HCV replicon cells.

116 adaptive mutations, and selected for stable replicon colonies.

117 e cellular diversity of subgenomic JFH-1 HCV replicons constitutively expressed in Huh7 cells.

118 5A alone from an additional cistron within a replicon construct gave greater rescue of virion assembl

119 fective concentration value for a GT-1a H77c replicon containing a Q30R substitution is ~7 nM, a rigo

120 Furthermore, replication of a HEV replicon containing green fluorescent protein (GFP) (E2-

121 patterns of luciferase expression from virus replicons containing the Gaussia luciferase gene in plac

122 nd PSI-353661 retained full activity against replicons containing the S282T substitution, which confe

123 ation fitness and sensitivity of various HCV replicons, containing or lacking NS2, to cyclosporine an

124 In the Huh-7 HCV genotype (GT) 1b replicon-containing cell line 9 is devoid of any anti-HC

125 ated antiviral activity upon transfer to HCV-replicon-containing cells.

126 ce gene to enable detection and selection of replicon-containing cells.

127 Cells containing the replicon could be cloned and passaged many times in the

128 While no resistant genotype 1a or 1b replicons could be selected, cells containing genotype 2

129 selected, cells containing genotype 2a JFH-1 replicons cultured in the presence of PSI-352938 or PSI-

130 construction of an improved broad-host-range replicon derived from RSF1010, which replicates in sever

131 This ability was also shared by a subgenomic replicon derived from the related GB virus B (GBV-B).

132 regulated at the level of large-scale multi-replicon domains.

133 We analyze how individual replicons dynamically organized a replication factory us

134 B enzyme (IC(50) = 0.008 muM) and cell-based replicon (EC(50) = 0.02 muM) assays and a good oral PK p

135 Compound 39 (GT1a replicon EC50 = 31 pM) has an extended plasma half-life

136 antiviral potency in genotype (gt) 1a and 1b replicons (EC50 = 120 and 110 pM, respectively) and with

137 he VSV glycoprotein gene is expressed from a replicon encoding the nonstructural proteins of Semliki

138 A modified replicon encoding West Nile virus (WNV) premembrane and

139 sed on engineered self-amplifying mRNA (SAM) replicons encoding an Ag, and formulated with a syntheti

140 kaging cell line can be transfected with HCV replicons encoding cognate Jc1-derived glycoprotein gene

141 sing a trans-complementation assay with JFH1 replicons encoding inhibitor-sensitive and inhibitor-res

142 ene deletion mutants suggests that secondary replicons evolved to fulfil a specialized function, part

143 (VLVs) are generated when an alphavirus RNA replicon expresses the vesicular stomatitis virus glycop

144 thermore, both genotype 1b and 2a subgenomic replicons expressing nonstructural (NS3-5B) proteins and

145 Testing replicons expressing representative envelope protein gen

146 We further demonstrate that HCV replicon-expressing cells initiate distinct tumor phenot

147 range of geminiviruses, advocate the use of replicons for plant genome engineering.

148 Here, we use geminivirus-based replicons for transient expression of sequence-specific

149 Conversely, curing of the replicon from these cells results in diminished expressi

150 e impact of SIL on replication of subgenomic replicons from different HCV genotypes in vitro and foun

151 trans-complementing genomes (referred to as 'replicons') from the arbovirus Sindbis [2].

152 eta interferons (IFN-alpha/beta) limit HuNoV replicon function, restrict MNV replication in cultured

153 -stereochemistry (EC50 = 3.4 +/- 0.2 pM, HCV replicon genotype 1b), was dramatically more active than

154 that sCD55 is induced in HCV-infected or HCV replicon-harboring cells, as well as in liver biopsy sam

155 DNA replication 50 years ago, the predicted replicons have not been identified and quantified at the

156 hat express luciferase-tagged subgenomic HCV replicons (Huh7/HCV replicon cells) or their HCV-negativ

157 Using an FMDV replicon in complementation experiments, our data demons

158 ate that the expression of an HCV subgenomic replicon in cultured cells results in the acquisition of

159 analyzed replication of the subgenomic JFH1 replicon in embryonic fibroblasts and primary hepatocyte

160 rus (HCV) genotype 1b strain Con1 subgenomic replicon in human hepatoma cells.

161 ary trajectories as its non-conjugative mini-replicon in the same and other species.

162 cells, as well as transfection of luciferase replicons in two types of cardiomyocytes, we demonstrate

163 90052, was investigated by evaluating hybrid replicons in which the entire NS5A coding region of geno

164 Using luciferase constructs/replicons, in vivo and in vitro assays showed that the 5

165 This replicon includes a green fluorescent protein gene and a

166 Analyses were conducted with replicons, infectious virus, and human hepatoma cells th

167 CV genotype 1a (G-1a) and genotype 1b (G-1b) replicon inhibition and selectivity against BVDV and cyt

168 gm for regulating the sites where individual replicons initiate replication.

169 roduction of virions that could transfer the replicon into most cell lines tested.

170 The fundamental unit of DNA replication, the replicon, is governed by a cis-acting replicator sequenc

171 y, transfected the cells with HCV subgenomic replicons lacking adaptive mutations, and selected for s

172 Even with subgenomic replicons, lacking structural viral proteins, exosome-me

173 ered in E1A-expressing cells, with increased replicon length, fork velocity, and interorigin distance

174 Replicon levels and drug-resistant variants were quantif

175 Use of a replicon library with codon degeneracy did allow identif

176 ns in the RSV replicon were not required for replicon maintenance.

177 Thus, genes on secondary replicons might potentially be manipulated to promote or

178 Since the pioneering proposal of the replicon model of DNA replication 50 years ago, the pred

179 Concurrent with the replicon model was Taylor's demonstration that plant and

180 on mechanism that departs from the classical replicon model, helping eukaryotic cells to negotiate tr

181 l principles and concepts established in the replicon model.

182 itional layers of regulation to the original replicon model.

183 wheat genome, and we show that with the WDV replicons, multiplexed GT within the same wheat cell can

184 However, the mutant form of a subgenomic replicon of genotype 3 HEV replicated more efficiently i

185 dence between a subgenomic and a full-length replicon of JFH-1 was due, at least in part, to an addit

186 setting of cellular division, when two viral replicons of equivalent fitness are present within a cel

187 NS2-5B replicons of genotype 2a (JFH1), but not genotype 1b, ha

188 -derived cell lines with subgenomic reporter replicons of HAV as well as of different HCV genotypes,

189 ication, which was observed using subgenomic replicons of two different genotypes.

190 They also show that the intragenomic replicon offers a unique way to study replication comple

191 lication were observed for specific LCV RNA2 replicons only in the presence of LCV RNA1, but both pro

192 n cells expressing a full-length genotype 1b replicon or infected with the JFH-1 strain of HCV.

193 harboring subgenomic hepatitis C virus (HCV) replicons or infected with cell culture-derived HCV were

194 levels of IFN-alpha after coculture with FL replicons or JFH-1-infected cells.

195 ce was found of persistent extra-chromosomal replicons or off-target integration of T-DNA or replicon

196 ction were assessed in HCV-permissive cells, replicons, or human embryonic kidney (HEK) 293 cells tra

197 While some behaved similarly to the S232I replicon, others displayed a unique trans-complementatio

198 e reverse genetics generation of a RVF virus replicon particle (VRP(RVF)) vaccine candidate.

199 by self-amplifying mRNA packaged in a viral replicon particle (VRP) or by a recombinant HIV envelope

200 (SUDV)- and Ebola virus (EBOV)-specific VEEV replicon particle (VRP) vaccines in nonhuman primates.

201 ressing Venezuelan equine encephalitis viral replicon particle vaccine protected these mice from SUDV

202 Kp47/47-Venezuelan equine encephalitis virus replicon particle) for safety, immunogenicity, and effic

203 ased on Venezuelan equine encephalitis virus replicon particles (VRP) expressing two configurations o

204 outs, but was largely inferior to virus-like replicon particles (VRP) or direct electroporation.

205 zuelan equine encephalitis virus (VEE) empty replicon particles (VRPs) can induce rapid protection of

206 tial vaccine [Venezuelan equine encephalitis replicon particles expressing MERS-CoV spike protein].

207 Inoculation of mice with WNV replicon particles resulted in high levels of replicatio

208 e nine alanine mutants affected the entry of replicon particles, which correlated with the impairment

209 production of both virus-like particles and replicon particles.

210 virus particles upon transfection with a GFP replicon plasmid.

211 mbination therapy was additive for the total replicon population and the LDV-resistant population, bu

212 Cell-based replicon potency was significantly improved through elec

213 Transpositions were also detected, revealing replicon preference (ISKpn18 prefers a conjugative IncA/

214 cutoff of 0.5% in genotype 1b-infected Con-1 replicons prior to in vitro treatment.

215 These replicons produce SG RNAs and encoded proteins of intere

216 significantly recovered the VP1 knockout MNV replicon replication, and the presence or absence of VP1

217 pression diminished the inhibitory effect on replicon replication, while overexpression of STING enha

218 lation between hyperphosphorylation and JFH1 replicon replication.

219 located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1).

220 orporating the newly determined L-IGR into a replicon reporter system enhanced the expression of a lu

221 es of 50 and 9 pM toward genotype-1a and -1b replicons, respectively, and oral bioavailabilities of 3

222 /L320F into genotype 1a (H77) and 1b (Con-1) replicons, respectively, increased the EC50 for mericita

223 phosphorylation of 4E-BP1 was stimulated by replicon RNA but not by UV-inactivated virus.

224 ed and indicated an average of 113 copies of replicon RNA per cell, correlating with calculated RNA c

225 sential for accumulation of GBV-B subgenomic replicon RNA.

226 mainder decreased the accumulation of a TBSV replicon RNA.

227 f JNK1 and JNK2, enhanced replication of HCV replicon RNAs as well as infectious genome-length RNA tr

228 licons or off-target integration of T-DNA or replicon sequences.

229 quantification of replication fork speed and replicon size in human and mouse cells.

230 Once associated, however, replicons stay together relatively stably to maintain re

231 tomato genome was achieved using geminivirus replicons, suggesting that these vectors can overcome th

232 treatment and those observed in the in vitro replicon system (major substitutions at residues 28, 30,

233 CC50 > 224 muM; SI > 28) in a cell based HCV replicon system assay.

234 ecific CD8 T cells have been shown in an HCV replicon system but not in an authentic infectious HCV c

235 evalence of hepatitis C virus genotype 4, no replicon system is available for study of the genotype.

236 We used a subgenomic replicon system to assess the effects of the same mutati

237 The genotype 4a replicon system we created will aid in the development o

238 ded by HCV NS3 protease assays, the cellular replicon system, structure-based design, and a panel of

239 25.6 muM, and CC50 > 180 muM in the Huh 9-13 replicon system, thus providing a good starting point fo

240 Making use of the Con1 HCV replicon system, we tested the effect of EMR proteins on

241 d not confer resistance to sofosbuvir in the replicon system.

242 ity of NK-cell subsets was studied using the replicon system.

243 ed in resistance development in the in vitro replicon system.

244 re further examined in the JFH-1 genotype 2a replicon system; importantly, all mutations that destabi

245 Reliance on hepatitis C virus (HCV) replicon systems and protein-based screening assays has

246 atitis E virus genotype 3 both in subgenomic replicon systems as well as a full-length infectious clo

247 -1 (HCVcc) and genotype 1b Con1b sub-genomic replicon systems were compared.

248 filoviruses but also for the design of other replicon systems widely used as surrogate systems to stu

249 NS5B polymerase that inhibit replication in replicon systems.

250 rally elicited a higher PCN of an IncP1-beta replicon than strains expressing TrfA2 alone.

251 We engineered an infectious TVCV replicon that expressed a functional fluorescence-tagged

252 ingle, synthetic self-replicating VEE-RF RNA replicon that expresses four reprogramming factors (OCT4

253 by deletion of the VSIV G gene to generate a replicon that is dependent on trans expression of G prot

254 tigate this possibility, we generated an RSV replicon that lacks all three of its glycoprotein genes

255 on of the 10 kGy IR-shattered D. radiodurans replicons that correlates with the timing of DraRnl repl

256 tform consisting of Semliki Forest virus RNA replicons that express the vesicular stomatitis virus gl

257 The "Replicon Theory" of Jacob, Brenner, and Cuzin has reliab

258 lls can be optically resolved down to single replicons throughout S-phase.

259 ) and sofosbuvir (SOF) against a genotype 1b replicon to determine optimal exposures for each agent t

260 d or completely abolished the ability of the replicon to replicate, further supporting the concept th

261 The contribution of each replicon to the microbial life cycle (for example, envir

262 Here, we use geminivirus replicons to create heritable modifications to the tomat

263 and RepB proteins direct the partitioning of replicons to daughter cells, while RepC proteins are rep

264 nstrate the feasibility of using geminivirus replicons to generate plants with a desired DNA sequence

265 These replicons transiently amplify to high copy numbers in pl

266 elegans based on the suppression of a viral replicon-triggered RDVI by ectopic expression of candida

267 a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1).

268 genome); substantial plasmid diversity (>/=9 replicon types); and substantial bla KPC-associated, mob

269 gly, pVAPN is a 120-kb invertron-like linear replicon unrelated to the circular virulence plasmids as

270 Previously, a replicon vaccine based on Venezuelan equine encephalitis

271 l replicon vaccines or the combination V/W/E replicon vaccine elicited strong neutralizing antibodies

272 l replicon vaccines or the combination V/W/E replicon vaccine elicited strong neutralizing antibodies

273 ntramuscular, Venezuelan equine encephalitis replicon vaccine expressing EBOV GP.

274 nd humoral immune response than the systemic replicon vaccine.

275 Individual replicon vaccines or the combination V/W/E replicon vacc

276 Individual replicon vaccines or the combination V/W/E replicon vacc

277 These data demonstrate that replicon vaccines successfully bridge the gap between sa

278 , we have extended the analysis with plasmid replicons, virulence factors, and highly discriminatory

279 nce typing, phylotyping, ESBL genes, plasmid replicons, virulence genes, amplified fragment length po

280 of an EBOV vaccine candidate based on Kunjin replicon virus-like particles (KUN VLPs) encoding EBOV g

281 plasmid DNA followed by boosting with Kunjin replicon virus-like particles both encoding a modified E

282 ics and Venezuelan equine encephalitis virus replicons (VRPs) expressing spike and nucleocapsid prote

283 Here, using the plasmid RK2 replicon, we analyze the protein interactions required f

284 However, in contrast with Con1 replicons, we observed a positive correlation between hy

285 Adaptive mutations in the RSV replicon were not required for replicon maintenance.

286 Alphavirus replicons were evaluated as potential vaccine candidates

287 HAV and HCV replicons were similarly sensitive to interferon (IFN),

288 The 53BP1 is preferentially bound to larger replicons, where the probability of DFSs is higher.

289 sted that the defective NS5A encoded by this replicon, while lacking one NS5A function, was capable o

290 , high frequencies of GT using WDV-based DNA replicons will make it possible to edit complex cereal g

291 An MNV replicon with a frameshift mutation in open reading fram

292 tro and replication of a sub-genomic HCV RNA replicon with a luciferase reporter in human hepatocarci

293 lpha did not repress transcription of an HBV replicon with a mutant HNF-4alpha binding site within en

294 Luciferase-expressing E7 sub-genomic replicons with CpGs and UpAs removed from the reporter g

295 Linear dsDNA replicons with hairpin ends are found in the three domai

296 ough effects on translation efficiency since replicons with high CpG/UpA sequences inserted into a no

297 In turn, research using RNA viruses as replicons with short generation times and high mutation

298 4t has excellent potency against the HCV 1b replicon, with an EC50 = 2 nM and a selectivity index of

299 uted by initiation at clusters of individual replicons within each segment.

300 Surprisingly, we show that the grouping of replicons within factories is highly variable from cell