ライフサイエンスコーパス: replisome

1 detection at sites of replication (i.e. the replisome).

2 rases function independently within a single replisome.

3 ntering a Tus-ter roadblock on an individual replisome.

4 advances in the structure of the eukaryotic replisome.

5 t connects multiple accessory factors to the replisome.

6 only capable of binding mismatches near the replisome.

7 uced by mutations that displace Pol from the replisome.

8 , triggered an interest in the dynamics of a replisome.

9 lation machinery, metabolic enzymes, and the replisome.

10 are concentrated and assembled into the HSV replisome.

11 how replication proteins enter and leave the replisome.

12 bonucleoside 5'-triphosphates (dNTPs) at the replisome.

13 echanisms to facilitate lesion bypass by the replisome.

14 n integral part of the minimal mitochondrial replisome.

15 DNA polymerase alpha (Pol alpha) within the replisome.

16 human polymerase and other components of the replisome.

17 s simply less processive in the context of a replisome.

18 oenzyme remains stably associated within the replisome.

19 the occupancy of polymerases within a moving replisome.

20 on of the leading-strand polymerase from the replisome.

21 hat keeps primase tethered to the eukaryotic replisome.

22 o coordinate enzymatic activities within the replisome.

23 he rate and fidelity of the Escherichia coli replisome.

24 contrast, RecA severely inhibits the Pol III replisome.

25 se to the Cdc45-MCM-GINS helicase within the replisome.

26 inds mismatches without associating with the replisome.

27 helicase, primase, and sliding clamp in the replisome.

28 ex forms an essential part of the eukaryotic replisome.

29 signed to either identical polymerase in the replisome.

30 in the same direction of replication by the replisome.

31 synthesis by the fully reconstituted Pol III replisome.

32 he conserved core of the archaeal/eukaryotic replisome.

33 ncounters a Tus-ter barrier before the other replisome.

34 ntifies a process unprecedented in bacterial replisomes.

35 nitiation and the overall functioning of DNA replisomes.

36 LS) is used to rescue progression of stalled replisomes.

37 , and processively as constituents of active replisomes.

38 plicative helicase is a crucial component of replisomes.

39 found in the Escherichia coli and eukaryotic replisomes.

40 sub-cellular localization of nascent DNA and replisomes.

41 e collisions of transcription complexes with replisomes.

42 their interaction with active leading-strand replisomes.

43 Duplication is concerted by replisomes.

44 Leading the replisome, a DNA helicase separates the parental strands

45 nome instability, because any loss of paused replisome activity creates a requirement for reloading o

46 Although the molecular basis for replisome activity has been extensively investigated, it

47 Unexpectedly, the replisome acts as an orientation-dependent regulator of

48 sential for Pol epsilon to interact with the replisome after initiation.

49 The replisome also coordinates nucleosome disassembly, assem

50 polymerases that are proximally bound to the replisome and continuously replenished from solution.

51 he organization of the catalytic core of the replisome and form an important step towards determining

52 t of Tus was most effective in arresting the replisome and mutation of C(6) removed the barrier.

53 4 histone chaperone that associates with the replisome and orchestrates chromatin assembly following

54 r data provide a quantitative picture of the replisome and replication stress response proteomes in 3

55 directional collisions occurring between the replisome and RNA polymerase.

56 ress the stalled fork in the presence of the replisome and SSB.

57 ress the stalled fork in the presence of the replisome and SSB; however, RuvAB generates a completely

58 elated with its separation distance from the replisome and that MutS motion slows when it enters the

59 45 clamp is continuously associated with the replisome and that no additional clamps accumulate on th

60 ecruitment and retention of Tof1-Csm3 by the replisome and that this complex antagonizes the Rrm3 hel

61 sites of collision between components of the replisome and the transcription apparatus and that it is

62 Imbalance of the dNTP pool also slows the replisome and thus is not specific to rNTPs.

63 rrest is manifested by a failure to assemble replisomes and by decreased rates of cell growth and rRN

64 system to comprehensively study thermophilic replisomes and evolutionary links between archaeal, euka

65 by checkpoint proteins that protect arrested replisomes and inhibit new initiation when replication i

66 ollisions between DNA replication complexes (replisomes) and barriers such as damaged DNA or tightly

67 between cellular DNA replication machinery (replisomes) and damaged DNA or immovable protein complex

68 between cellular DNA replication complexes (replisomes) and obstacles such as damaged DNA or frozen

69 ication process, wherein contacts within the replisome are continually broken and reformed.

70 d events that impart negative effects on the replisome are counterbalanced by the positive effects of

71 are well understood, its dynamics within the replisome are not.

72 Two replisomes are assembled, one on each strand, and move i

73 Replisomes are multiprotein complexes that coordinate th

74 and the proteins that work at the fork (the replisome) are known targets for the signaling pathways

75 During origin firing in S phase, replisomes assemble around the activated Mcm2-7 DNA heli

76 nitiation factor Mcm10 is essential for both replisome assembly and function.

77 Replisome assembly at eukaryotic replication forks conne

78 ication initiates where forks meet through a replisome assembly mechanism normally associated with fo

79 Replisome assembly requires the loading of replicative h

80 element (DUE) contribute to strand-specific replisome assembly.

81 and to melt the DNA helix in preparation for replisome assembly.

82 We now show that, in yeast, the replisome-associated components Tof1 and Csm3 physically

83 We show that replisome-associated factors Mrc1 and Csm3/Tof1 are cruc

84 protein complexes and place newly identified replisome-associated proteins into functional pathways.

85 HR has the ability to rebuild a replisome at inactivated forks, but perhaps surprisingly

86 eading-strand synthesis by the S. cerevisiae replisome at the single-molecule level.

87 of a DNA replication complex (break-induced replisome) at telomeres or elsewhere in the mammalian ge

88 polymerase does not result in loss from the replisome because of its contact with the leading-strand

89 tion fork progression is slowed down and the replisome becomes unstable in the presence of hydroxyure

90 that MutS dynamically moves to and from the replisome before mismatch binding to scan for errors.

91 r immovable protein complexes can dissociate replisomes before the completion of replication.

92 ing replication fork stalling stabilizes the replisome, but how these modifications safeguard the for

93 strand template only transiently stalls the replisome, but it too is degraded, allowing Okazaki frag

94 These MutS foci are directed to the replisome by DnaN clamp zones that aid mismatch detectio

95 ze this coordination in the bacteriophage T7 replisome by simultaneously monitoring the kinetics of l

96 C on the leading strand template arrests the replisome by stalling the CMG helicase.

97 lymerase III holoenzyme in a stalled E. coli replisome can directly bypass a single cyclobutane pyrim

98 ding the molecular basis for how the E. coli replisome can maintain high processivity and yet possess

99 Although the yeast replisome can overcome RF pausing at Tus-Ter modules, th

100 es, and assembly in vitro and in vivo into a replisome capable of coordinated leading/lagging strand

101 The results reveal a dynamic replisome, capable of partial disassembly to allow acces

102 t as a loader protein for the recruitment of replisome cascade proteins.

103 The instability of the replisome complex is conflict-induced: transcription inh

104 Here, we characterize the replisome-complex stoichiometry and dynamics with single

105 t with the idea that polyubiquitylation of a replisome component (Mcm7) leads to its disassembly at t

106 We show that the mammalian replisome component C20orf43/RTF2 (homologous to S. pomb

107 at replication protein A (RPA), an essential replisome component that binds single-stranded DNA, has

108 We demonstrate that DONSON is a replisome component that stabilizes forks during genome

109 Here, we demonstrate that And-1, a replisome component, acts together with ATR to activate

110 We conclude that the stochastic behavior of replisome components ensures complete DNA duplication wi

111 ed for the recruitment of firing factors and replisome components in the extract.

112 Recent structures of eukaryotic replisome components include the Mcm2-7 complex, the CMG

113 provide evidence that Rtt107 associates with replisome components, and both itself and its associated

114 axis, but is independent of other canonical replisome components, ATM and ATR, or the homologous rec

115 otential regulators of termination, and many replisome components.

116 stituted a eukaryotic leading/lagging strand replisome comprising 31 distinct polypeptides.

117 searched for functional interactions in the replisome context by subjecting HSV-1 UL8 protein to ext

118 Finally, when two replisomes converge they are disassembled.

119 tivation, but how specific components of the replisome coordinate with ATR to activate Chk1 in human

120 , Polzeta-dependent mutagenesis triggered by replisome defects or UV irradiation in vivo was not decr

121 vation occurs in strains, in which intrinsic replisome defects promote the participation of error-pro

122 Hybrid replisomes derived from herpes simplex virus type 1 (HSV

123 r data provide insight into the mechanism of replisome disassembly during eukaryotic DNA replication

124 identify CRL2(Lrr1) as a master regulator of replisome disassembly during vertebrate DNA replication

125 ve short lifetimes (<8 min), suggesting that replisome disassembly is quite prevalent, possibly occur

126 be rescued in a manner that does not involve replisome disassembly or reassembly, albeit with loss of

127 on of DNA synthesis while avoiding premature replisome disassembly.

128 esis, decatenation of daughter molecules and replisome disassembly.

129 Individual replisomes display both looping and pausing during primi

130 nd helicase stay together at the lesion, the replisome does not dissociate and the helicase does not

131 idelity and translesion synthesis within the replisome during DNA replication.

132 t two or more polymerases are present in the replisome during DNA replication.

133 * subassembly frequently disengages from the replisome during DNA synthesis and exchanges with free c

134 AC mass spectrometry, we characterized human replisome dynamics in response to fork stalling.

135 proteins form a stable complex, known as the replisome, enabling them to act together in a highly coo

136 plexes are one of the principal barriers the replisome encounters during replication.

137 We show that when a replisome encounters the lesion, a substantial fraction

138 that is positioned such that one of the two replisomes encounters a Tus-ter barrier before the other

139 that aid resumption of replication by paused replisomes, especially those halted by protein-DNA barri

140 mismatch detection, MutS disengages from the replisome, facilitating repair.

141 Saccharomyces cerevisiae pre-RC assembly and replisome firing in real time.

142 viding insights into the recruitment of host replisome for viral DNA replication.

143 Our investigations revealed that in E. coli, replisome formation at the plasmid origin involves inter

144 (ORC), a six-subunit assembly that promotes replisome formation on chromosomal origins.

145 coli sliding clamp is a protein mediator of replisome formation, which uses a common surface pocket

146 ate nascent replication bubbles, and promote replisome formation.

147 Eukaryotic DNA replication terminates when replisomes from adjacent replication origins converge.

148 ghtly bound protein complexes can dissociate replisomes from chromosomes prematurely.

149 ibition is a multistep process that disrupts replisome function and permits cleavage of the replicati

150 on the importance of B-subunit integrity in replisome function in vivo.

151 pancy of multiple DNA polymerases within the replisome has been observed primarily in bacteria and is

152 The T4 replisome has provided a unique opportunity to investiga

153 the roles of the various proteins within the replisome have been determined.

154 In vitro studies of reconstituted E. coli replisomes have attributed this remarkable processivity

155 avior and interactions in the context of the replisome, however, remain unclear.

156 colocalizes with the DNA polymerase complex (replisome) immediately following DNA damage or damage-in

157 sistent with the presence of only one active replisome in a significant fraction of cells (>40%).

158 ht into the organization and dynamics of the replisome in bacterial cells.

159 H3/H4 tetramer suggest a direct role of the replisome in handling nucleosomes, which are important t

160 ilizes these complexes, restoring the second replisome in many of the cells.

161 terized the dynamic movement of MutS and the replisome in real time using superresolution microscopy

162 ndicates that the protein accumulates at the replisome in sporulating cells, likely through a direct

163 of primase being tethered to the eukaryotic replisome in this way.

164 ding is critical for MutS to localize to the replisome in vivo.

165 e Ctf4 partner DNA polymerase alpha from the replisome in yeast extracts.

166 By examining replisomes in live E. coli with fluorescence microscopy,

167 Our data reveal a peripheral localization of replisomes in the cell.

168 ese findings reveal the hidden potential for replisome inactivation, and hence genome instability, in

169 rate that most pausing events do not lead to replisome inactivation, that transcription complexes are

170 is process was mediated by the mitochondrial replisome independent of canonical DSB repair.

171 ading strand lesion skipping activity of the replisome, indicating that they are competing reactions.

172 erminal DHH domain, which appears poised for replisome interactions.

173 e inherent risk of genome instability, since replisomes invariably encounter DNA lesions or other str

174 The eukaryotic replisome is a critical determinant of genome integrity

175 The eukaryotic replisome is a molecular machine that coordinates the Cd

176 The replisome is a multiprotein machine that carries out DNA

177 ds, we demonstrate that the bacteriophage T7 replisome is able to directly replicate through a leadin

178 role of the hCMG complex as the core of the replisome is also discussed.

179 The bacteriophage T7 replisome is an economical machine that requires only fo

180 uced rate after replisome stalling, that one replisome is capable of skipping multiple lesions, and t

181 Assembly of the T7 replisome is driven by intimate interactions between the

182 The B. subtilis replisome is eukaryotic-like in that it relies on a two

183 These observations suggest that the T7 replisome is fundamentally permissive of DNA lesions via

184 Nevertheless, the replisome is highly resistant to dilution in the absence

185 replication of undamaged DNA when the normal replisome is impaired.

186 The replisome is important for DNA replication checkpoint ac

187 rks, but perhaps surprisingly, the resulting replisome is liable to intrastrand and interstrand switc

188 he SSB-dependent recruitment of RecOR to the replisome is necessary for loading and organizing RecA i

189 Furthermore, the replisome is only transiently blocked, and continues rep

190 constituted DNA replication system where the replisome is stalled by collision with leading-strand te

191 constituted DNA replication system where the replisome is stalled by collision with leading-strand te

192 n of alpha, beta, or tau subunits within the replisome is sufficient to signal and induce the RecF-me

193 ial component of the human mitochondrial DNA replisome is the ring-shaped helicase TWINKLE-a phage T7

194 The function of the resulting replisomes is monitored by checkpoint proteins that prot

195 In Escherichia coli, a single pair of replisomes is responsible for duplicating the entire 4.6

196 ex acts in a dynamic fashion with the moving replisome, leading to alternating phases of slow and fas

197 By analyzing chromosome and replisome localization, we demonstrated that chromosome

198 ng proteins associated with helicases in the replisome may have coevolved with helicases to increase

199 d single-molecule methods, and show that the replisome may solve the topological problem independent

200 Recruiting regulatory kinases to replisomes may be a general mechanism to ensure spatial

201 Escherichia coli replisome movement along transcribed DNA is promoted by

202 nsures that stalled forks remain stable when replisome movement is impeded.

203 ading- and lagging-strand polymerases in the replisome must be coordinated to avoid the formation of

204 Replisomes must be reloaded under these circumstances to

205 chinery responsible for DNA replication, the replisome, must efficiently coordinate DNA unwinding wit

206 Ctf4 trimer hub and the first look at a core replisome of 20 different proteins containing the helica

207 ture of the approximately 650-kDa functional replisome of bacteriophage T7 assembled on DNA resemblin

208 resembles the emerging model for the simpler replisome of Escherichia coli.

209 Replication reactions by the T4 replisome on this substrate yielded a patterned series o

210 le processivity to the high stability of the replisome once assembled on DNA.

211 n restart mechanisms that function to reload replisomes onto abandoned DNA replication forks.

212 is and revealing an underlying plasticity in replisome operation.

213 mere damage recognition by the break-induced replisome orchestrates homology-directed telomere mainte

214 eats but was most frequently accomplished by replisomes originating in the subtelomere.

215 minimizing the frequency and/or duration of replisome pauses.

216 Using RecD2 to probe replisome pausing in vivo, we demonstrate that most paus

217 plicative helicases are the motor engines of replisomes powered by the conversion of chemical energy

218 These collisions can lead to the ejection of replisomes prior to completion of replication, which, if

219 We find that primase activity lowers replisome processivity but only when lagging strand exte

220 rNTPs also lower replisome processivity.

221 merases can alleviate potential obstacles to replisome progression by facilitating DNA lesion bypass,

222 Instead, the tethering of SCF(Dia2) to the replisome progression complex increases the efficiency o

223 racts with the Ctf4 and Mrc1 subunits of the replisome progression complex, which assembles around th

224 e activity is sufficient to maintain regular replisome progression in unperturbed cells.

225 in the context of chromatin, but subsequent replisome progression requires the histone chaperone FAC

226 e data suggest that PolY1 promotes efficient replisome progression through lagging-strand genes, ther

227 ondary structures at telomeres to facilitate replisome progression.

228 template engagement and release, modulating replisome progression.

229 d Csm3/Tof1 are crucial for in vivo rates of replisome progression.

230 We show that Dna2 associates with the replisome protein And-1 in a cell cycle-dependent manner

231 inhibits Twinkle unwinding, suggesting other replisome proteins may be required for efficient removal

232 rating cell nuclear antigen (PCNA) and other replisome proteins on the chromatin during and even afte

233 eplication factories with an accumulation of replisome proteins.

234 of Pol alpha to one CMG helicase within the replisome, providing a new model for lagging-strand synt

235 ted to and released from a centrally located replisome, providing, to our knowledge, new insight into

236 agging strand events on the Escherichia coli replisome rate and processivity.

237 an leading strand synthesis, indicating that replisome rate is limited by the helicase.

238 hways that do not require fork adjustment or replisome reassembly.

239 ation moves to and transiently dwells at the replisome region, even in the absence of appreciable mis

240 nd that MutS motion slows when it enters the replisome region.

241 tion and reveal a novel mechanism of how the replisome regulates the replication checkpoint and genom

242 PriA helicase catalyzes the initial steps of replisome reloading onto repaired DNA replication forks

243 owever, the fate and behavior of the stalled replisome remains a central uncharacterized question.

244 When stress arrests replication, the replisome remains associated with the fork DNA (stalled

245 We demonstrate that the replisome remains stably bound after encountering a Tus-

246 hese pathways include lesion skipping by the replisome, replication fork regression followed by eithe

247 The minimal reconstituted leading-strand replisome requires 24 proteins, forming the CMG helicase

248 use thermosensitive mutants to show that the replisome's polymerases uncouple and transiently dissoci

249 capable of dynamic movement to and from the replisome, showing that proper nucleotide binding is cri

250 The eukaryotic core replisome shows an unanticipated architecture, with one

251 for the TPR domain of Dia2, either mediating replisome-specific degradation of Mrc1 and Ctf4 or else

252 ere, we show that SCF(Dia2) does not mediate replisome-specific degradation of Mrc1 and Ctf4, either

253 ulated to contribute to fork progression and replisome stability.

254 stream of the lesion at a reduced rate after replisome stalling, that one replisome is capable of ski

255 The E. coli replisome stalls transiently when it encounters a lesion

256 , but Mcm10 is not a stable component of the replisome subsequently.

257 ir individual functions, including a role in replisome sumoylation.

258 to the DNA replication machinery (i.e., the replisome) than others.

259 ed, and a large subcomplex of the vertebrate replisome that includes DNA Pol epsilon is retained on D

260 This produces a replisome that lacks DNA polymerase epsilon, and althoug

261 WDHD1 is a component of the replisome that regulates DNA replication.

262 RecD2 helicase inactivates Escherichia coli replisomes that are paused but still functional in vitro

263 ly, we find that RecA specifically activates replisomes that contain TLS Pols.

264 otic cells, the assembly of the multi-enzyme replisomes that perform replication is divided into stag

265 Importantly, rather than stabilize the replisome, the checkpoint prevents two distinct types of

266 y another key component of the mitochondrial replisome, the mitochondrial single-stranded DNA-binding

267 The replisome, the multi-protein machinery responsible for c

268 The replisome, the multiprotein system responsible for genom

269 richia coli, the proofreading subunit of the replisome, the varepsilon exonuclease, is essential for

270 MTC) enhances the rate of the leading-strand replisome threefold.

271 ablished that RTEL1 also associates with the replisome through binding to proliferating cell nuclear

272 hat are crucial for unimpeded passage of the replisome through various barriers and difficult to repl

273 ks and resumption of replication by the same replisome thus circumventing the need for replication re

274 ost significantly, Mcm10 enables CMG and the replisome to bypass blocks on the non-tracking DNA stran

275 ngage productively with the Escherichia coli replisome to bypass leading strand template damage, desp

276 in principle, allow DnaB and the associated replisome to continue DNA synthesis without impediment,

277 a2) (SCF [Skp1/cullin/F-box protein]) to the replisome to increase its local concentration at replica

278 enome integrity relies on the ability of the replisome to navigate ubiquitous DNA damage during DNA r

279 The ability of the replisome to seamlessly coordinate both high fidelity an

280 study responses of the heterologous phage T7 replisome to the Tus-Ter complex.

281 hat it is a constituent of the mitochondrial replisome, to which it provides an additional exonucleas

282 junction with immunolocalization analyses of replisomes, to investigate the sub-cellular localization

283 The Escherichia coli replisome transiently stalls at leading-strand template

284 The replisome unwinds and synthesizes DNA for genome duplica

285 examine the dynamics of the Escherichia coli replisome, using ensemble and single-molecule methods, a

286 that Ctf4 recruits the Chl1 helicase to the replisome via a conserved interaction motif that Chl1 sh

287 The T7 replisome was arrested at the non-permissive end of Tus-

288 lC proteins within each cell and within each replisome, we elucidate the diffusion characteristics of

289 opposite effects of RecA on Pol III and TLS replisomes, we propose that RecA acts as a switch to reg

290 crofuidics, we investigate the effect on the replisome when encountering these barriers in live Esche

291 The details concerning the dynamics of the replisome when encountering these Tus-ter barriers in th

292 Mec1 and Rad53 might prevent collapse of the replisome when nucleotide concentrations are limiting, t

293 A) is situated at the core of the eukaryotic replisome, where it acts as an interaction scaffold for

294 dent Cdc7 kinase (DDK) and recruit it to the replisome, where it phosphorylates the DSB-promoting fac

295 d demonstrate that it utilizes a specialized replisome, which underlies ALT telomere maintenance.

296 bacteriophage T7 DNA polymerases within the replisome while we simultaneously observe the kinetics o

297 tudies imply a highly dynamic picture of the replisome with lagging-strand DNA polymerases residing a

298 rcome these challenges, we paused converging replisomes with a site-specific barrier in Xenopus egg e

299 conclude with a brief comparison with other replisomes with emphasis on how coordinated DNA replicat

300 ation fork can be replicated directly by the replisome without the need to activate error-prone pathw