ライフサイエンスコーパス: reporter assay

1 sion level of AQP4 in an in vitro luciferase reporter assay.

2 omoter activity was measured in a luciferase reporter assay.

3 vate STAT3-binding element in the luciferase reporter assay.

4 ive polymerase chain reaction and luciferase reporter assay.

5 d and unmethylated promoters in a luciferase reporter assay.

6 w regulatory activity in an Escherichia coli reporter assay.

7 s was confirmed by qPCR and 3'UTR luciferase reporter assay.

8 65 by bioinformatics analysis and luciferase reporter assay.

9 lated region of these genes was confirmed by reporter assay.

10 85 and miR-186 was confirmed by a luciferase reporter assay.

11 TGFB1 transcriptional activity by luciferase reporter assay.

12 separated AF-1 and AF-2 using a novel hybrid reporter assay.

13 sessed by RT-qPCR, ChIP assay and luciferase reporter assay.

14 lates, and simultaneously evaluated in a p53 reporter assay.

15 ion, as assessed by a bicistronic luciferase reporter assay.

16 SNP on alternative splicing using a minigene reporter assay.

17 arget validation was confirmed by luciferase reporter assay.

18 uced pluripotent stem cell (hiPSC)-based RPE reporter assay.

19 a target of miR-377 using a dual luciferase reporter assay.

20 tly targeted by miR-124-3p with a luciferase reporter assay.

21 upported by the results of a dual-luciferase reporter assay.

22 hemokine 3'-UTRs that destabilized mRNA in a reporter assay.

23 ghly conserved IEC expression in a zebrafish reporter assay.

24 -/-) cells than in wild-type (WT) cells in a reporter assay.

25 tion ability as demonstrated by a luciferase reporter assay.

26 diated RNAi suppression indicated by the GFP reporter assay.

27 rmed by Western blot analysis and luciferase reporter assay.

28 on reduced CYP11B1 promoter activity using a reporter assay.

29 cing, and their function was tested by using reporter assays.

30 ng confirms the fidelity effects seen in our reporter assays.

31 ked with mRNA expression data and transgenic reporter assays.

32 ociated target gene (SORL1) using luciferase reporter assays.

33 s finding was confirmed using the luciferase reporter assays.

34 sing exogenous transfection-based luciferase reporter assays.

35 eased transcriptional activity in luciferase reporter assays.

36 on of known and novel targets using targeted reporter assays.

37 ore than 60% as shown by PPARgamma-dependent reporter assays.

38 effect on IRF3-dependent gene expression in reporter assays.

39 of dense mutagenesis of several enhancers in reporter assays.

40 r activity in AP-1- and NF-kappaB-luciferase reporter assays.

41 as measured using allele-specific luciferase reporter assays.

42 eased translation efficiencies in luciferase reporter assays.

43 UTR, but not promoter, activity, as shown by reporter assays.

44 chromatin immunoprecipitation and luciferase reporter assays.

45 as direct miR-24 targets through luciferase reporter assays.

46 enuated repair by both NHEJ and HR in HEK293 reporter assays.

47 of the ERalpha in a PTB2-dependent manner in reporter assays.

48 RORgamma- or RORgammat-dependent cell-based reporter assays.

49 ptors in both yeast and mammalian cell-based reporter assays.

50 resulted in decreased luciferase activity in reporter assays.

51 ting the results of chromosomally integrated reporter assays.

52 bioinformatics analyses and dual-luciferase reporter assays.

53 , chromatin immunoprecipitation and promoter reporter assays.

54 o identify enhancers previously validated in reporter assays.

55 mpens SPIN1 coactivator activity in TOPflash reporter assays.

56 acterized at functional level using in vitro reporter assays.

57 p, 13 candidates were screened in luciferase reporter assays.

58 transcription and replication in luciferase reporter assays, a mutant that may act as a phosphomimet

59 Immunohistochemistry, immunoblotting, and reporter assays all show a significant activation of the

60 Chromatin immunoprecipitation (ChIP) and reporter assays also indicate that the ARC gene is regul

61 Luciferase-based reporter assays also revealed that the binding domain of

62 A luciferase reporter assay and a pulldown assay using biotinylated I

63 A HEK-IL-1 reporter assay and brain endothelial cell line were used

64 Reporter assay and chromatin immunoprecipitation studies

65 Using dual-luciferase reporter assay and Chromatin immunoprecipitation, we dem

66 it activates gene expression in a luciferase reporter assay and following retroviral transduction.

67 n the mouse Sox9 promoter using a luciferase reporter assay and gel shift and ChIP studies.

68 PARgamma1 transcriptional activity in a Cos7 reporter assay and induced lipid accumulation and perili

69 Additionally, an in vivo promoter reporter assay and motility analysis revealed a key role

70 p < 0.003) between data collected from this reporter assay and our previous ligand binding assay tes

71 g computational network analysis, an in vivo reporter assay and physiological validation experiments.

72 G) and selected compounds using a luciferase reporter assay and predictions through molecular docking

73 w transcriptional activation in a cell-based reporter assay and was associated with diminished IL23R

74 wed robust promoter activity by a luciferase reporter assay and was inhibited by in vitro artificial

75 n TGF-beta signaling using a SMAD-luciferase reporter assay and Western blotting for phospho-SMAD2/3

76 Using reporter assays and cell-based studies, we demonstrate t

77 urately predicts translation efficiencies in reporter assays and improves alpha-1-antitrypsin express

78 ico predicted target genes were confirmed in reporter assays and in house-dust-mite (HDM) induced AAI

79 The results of dual-luciferase reporter assays and in vitro studies both indicated that

80 at inhibits Wnt/beta-catenin activity in Wnt reporter assays and in Wnt-dependent mesoderm differenti

81 Here we use a combination of yeast reporter assays and mammalian systems to provide a more

82 lts from unbiased RISC-trap screens, in vivo reporter assays and overexpression studies indicated tha

83 In splicing reporter assays and pre-clinical patient-derived AML mod

84 ed with increase in transcript expression in reporter assays and primary samples.

85 Moreover, using reporter assays and protein synthesis assays, we show th

86 n consequences were assessed with luciferase reporter assays and real-time quantitative polymerase ch

87 UTR-seq, a combination of massively parallel reporter assays and regression models, to survey the dyn

88 1-2-5p was confirmed using 3'-UTR luciferase reporter assays and Western blot assays.

89 skolin-induced PKA activation (measured by a reporter assay) and an impaired ability of cAMP to disso

90 n activating p53 on apoptotic promoters in a reporter assay, and c-Abl was required for endogenous HI

91 Using a combination of bioinformatics, reporter assay, and chromatin immunoprecipitation analys

92 RNA (mRNA) decay using genetic approaches, a reporter assay, and high-throughput degradome profiles.

93 rvation, tissue-specific chromatin, in vitro reporter assay, and in vivo transgenic data to identify

94 rator-activated receptor gamma in luciferase reporter assay, and PPAR-gamma selective antagonist comp

95 ulate KRAS promoter activity in a luciferase reporter assay, and reduce both KRAS mRNA and p21(KRAS)

96 d as a target gene of miR-200a by luciferase reporter assay, and upregulation of miR-200a significant

97 Bioinformatic, luciferase reporter assay, and Western blot analyses identified Rho

98 ariants, antibody-mediated cell cytotoxicity reporter assays, and Fcgamma receptor-deficient (Fcer1g(

99 qPCR,luciferase reporter assays, and western blot also verified the ATP

100 ity of mouse BAFF on a variety of cell-based reporter assays; and antagonized the prosurvival action

101 The results of chromosomally based reporter assays are also more reproducible and more stro

102 A longstanding concern is that reporter assays are mainly implemented on episomes, whic

103 3'-Untranslated region reporter assays, argonaute-2 microribonucleoprotein immu

104 ivate the E-cadherin gene CDH1 in a promoter reporter assay as a measure of EMT reversal.

105 assessed using different T-cell factor (TCF) reporter assays as a readout for Wnt/beta-catenin-depend

106 RNA-seq and reporter assays, as described here, may help to further

107 n response to TNF was measured by luciferase reporter assays; binding of the NF-kappaB subunit p65 in

108 NA datasets with TFBS prediction and in vivo reporter assays can reveal tissue-specific regulatory in

109 staining, transient transfection, luciferase reporter assay, chromatin immunoprecipitation assay, gel

110 , western blotting, PCR, promoter-luciferase reporter assays, chromatin immunoprecipitation and pull-

111 Luciferase reporter assays confirm that p53 drives POX transcriptio

112 77; RNA immunoprecipitation and a luciferase reporter assay confirmed that ilp7 and ilp8 are direct t

113 Luciferase reporter assay confirmed that miR-133a targets TAT.

114 A luciferase reporter assay confirmed that the 3'-untranslated region

115 g site, and further studies using luciferase reporter assays confirmed Gli1-dependent promoter activi

116 Reporter assays confirmed that cap-distal stem-loop inse

117 Luciferase reporter assays confirmed that miR-297c-5p targets cycli

118 immunoprecipitation sequencing and promoter reporter assays confirmed that SOX11 directly binds to i

119 Subsequent 3' untranslated region luciferase reporter assays confirmed that the translation of both a

120 Immunoblotting and luciferase reporter assays confirmed that TIP30 was a direct miR-10

121 Posttranscriptional reporter assays confirmed the functionality of AREs.

122 Luciferase reporter assays confirmed the interaction between miR-12

123 Luciferase reporter assays confirmed the predicted interactions bet

124 pressed by miR-206 but not miR-9 in a 3'-UTR reporter assay, confirming BDNF as a functional target o

125 Dual-luciferase reporter assay confirms that ADAM9 3'UTR contains miR-12

126 etween +4517 and 4662 bp, but the luciferase reporter assay demonstrated that this enhancer is not Sm

127 Dual-luciferase reporter assays demonstrated that FGFRL1 3' untranslated

128 Bacterial two-hybrid and gene-reporter assays demonstrated that FseA was also bound an

129 in immunoprecipitation (ChIP) and luciferase reporter assays demonstrated that hGRbeta binds to the i

130 Luciferase reporter assays demonstrated that high miR-375 expressio

131 Luciferase reporter assays demonstrated that miR-21 produced a conc

132 Reporter assays demonstrated that rs9920291 shows alleli

133 Reporter assays demonstrated that this extended region r

134 Transgenic reporter assays demonstrated that this polyC motif is re

135 Luciferase reporter assay demonstrates that SMAD3 transactivates S1

136 Using a reporter assay designed to study G4-induced recombinatio

137 and analyzed on immunoblots or in luciferase reporter assays designed to measure TNF activity.

138 Our data indicate that the stable CB reporter assays detect CB receptor activation by extract

139 Utilizing cell-based luciferase reporter assays, electrophoretic mobility shift assays,

140 yse elt-2 gene regulation through transgenic reporter assays, ELT-2 ChIP and characterisation of in v

141 lectrophoretic mobility shift and luciferase reporter assays examined the binding and functionality o

142 Nevertheless, designing high-throughput reporter assay experiments such as massively parallel re

143 pha-SYN expression; while exogenous promoter-reporter assays failed to reproduce the similar outcomes

144 h factor 9 (FGF9) expression in a luciferase reporter assay, FGF9 levels were actually increased in G

145 omoter activity was observed in a luciferase reporter assay for rs2395471_G relative to rs2395471_A o

146 otated alternative promoters, and luciferase reporter assays for three of four of these promoters con

147 The luciferase reporter assay further verified that the miR-103 and miR

148 BPAF, Coum, 1-BP) of 16 compounds tested by reporter assay had estrogenic activity through mERbeta2.

149 Many studies using reporter assays have demonstrated that 3' untranslated r

150 n HEK293T cell-based transfection/luciferase reporter assays, heightened interleukin-4 (IL-4) -induce

151 Luciferase reporter assays highlighted a 173-bp region of CXCL8/IL-

152 ed for Glo1 inducer activity in a functional reporter assay, hits were confirmed in cell culture, and

153 ChIP and Luciferase Reporter assays identified that EP1 agonist treatment in

154 Bioinformatic TargetScan and reporter assays identified the binding of miR-16 and miR

155 re both predictive of regulatory function in reporter assays, identified retroviral elements with act

156 RNA-immunoprecipitation and luciferase reporter assays illustrated that both PTB and miR-221 bi

157 e established a miniaturized luciferase gene reporter assay in A549 cells that measures IFN-beta indu

158 oyed a Piwi-interacting RNA (piRNA)-targeted reporter assay in Drosophila ovary somatic sheet (OSS) c

159 Here, we generated a genetic reporter assay in pluripotent stem cells using newly-dev

160 However, using dual-luciferase reporter assay in SH-SY5Y cells we showed that Wnt signa

161 urther demonstrated using an IL-9 luciferase reporter assay in which BCL6 repressed STAT5-mediated Il

162 Reporter assays in Drosophila S2 cells further revealed

163 Cell-based fluorescence reporter assays in Escherichia coli revealed that mutati

164 ChIP and reporter assays in HeLa cells with monoallelic CD177 exp

165 We used luciferase reporter assays in HepG2 cells to test all 25 variants f

166 Reporter assays in human embryonic kidney 293 cells show

167 We used time-resolved reporter assays in living yeast cells to gain insights i

168 ression and knockdown studies and luciferase reporter assays in mouse and human hepatic cells.

169 Quantitative reporter assays in oral epithelial and thyroid cell line

170 an islets, lower transcriptional activity in reporter assays in rodent beta-cells (rat 832/13 and mou

171 Overexpression assays in zebrafish and reporter assays in vitro indicated that 4 variants were

172 tly enhance Il2 transcription in recombinant reporter assays in vitro, and the native region undergoe

173 pecificity of TRUB1 using massively parallel reporter assays in which we monitored Psi levels at thou

174 Transgenic reporter assays in zebrafish confirm enhancer activities

175 tion in an orientation-independent manner in reporter assays, in the native chromosome context, the o

176 Bioinformatics analysis and dual-luciferase reporter assay indentified LHX6 as a direct target gene

177 purification (TRAP) and in vitro luciferase reporter assay indicate that mir-92a suppresses expressi

178 Gene reporter assays indicate that relocating Rho target elem

179 Dual luciferase reporter assay indicated that MdCCA1 but not MdCSP3 acti

180 The reporter assay indicated that target-site mutation resul

181 The luciferase reporter assay indicated that the 3' untranslated region

182 RNA sequencing data and a uidA reporter assay indicated that the MybA protein functions

183 Reporter assays indicated that direct interaction of miR

184 Both RNA-seq and in vitro reporter assays indicated that enhancer hypermethylation

185 Of note, reporter assays indicated that IRF5 re-expression inhibi

186 Chromosome configuration capture and reporter assays indicated that IRF6 directly regulates a

187 tion with low micromolar IC50s in cell-based reporter assays, inhibit Gas6-inducible motility in Axl-

188 a novel lentivirus-based massively parallel reporter assay (lentiMPRA) to directly compare the funct

189 A cell-based reporter assay linking LasR function with beta-galactosi

190 challenge, we adapted the massively parallel reporter assay (MPRA) to identify variants that directly

191 We employ a massively parallel reporter assay (MPRA) to simultaneously screen 2,756 var

192 of the HLA-G locus using massively parallel reporter assay (MPRA) uncovered a previously unidentifie

193 n interaction sequencing, massively parallel reporter assays (MPRA), and transgenic mice, we identifi

194 assay experiments such as massively parallel reporter assays (MPRAs) and similar methods remains chal

195 quences using a series of massively parallel reporter assays (MPRAs) coupled with the assay for trans

196 Massively parallel reporter assays (MPRAs) enable nucleotide-resolution dis

197 To address this issue, massively parallel reporter assays (MPRAs) have emerged, in which barcoded

198 Here, we used massively parallel reporter assays (MPRAs) involving 32,115 natural and syn

199 We developed a robust cellular cAMP reporter assay of RXFP1 signaling in HEK293 cells transi

200 yridine 9 using a high-throughput cell-based reporter assay of WNT pathway activity.

201 combined with proteome arrays and luciferase reporter assays of miR-193a-3p mimic treated cord blood

202 through marker exchange mutagenesis and lacZ reporter assays of the promoter for genes encoding this

203 A luciferase reporter assay on a PXR-transfected HepG2 cell line iden

204 Luciferase reporter assays on rs4888378 showed a significant 35% to

205 ated both by RNA sequencing (P < 0.0001) and reporter assays (P = 0.002).

206 on and with an in vitro MHC class II peptide reporter assay performed in parallel, which used synthet

207 A luciferase reporter assay proves that Gsdma3 directly suppresses Wn

208 ated region (3'-UTR) targets with luciferase reporter assay provided a more favorable result for miR-

209 Dual-luciferase reporter assay revealed that bta-miR-23a directly target

210 Luciferase reporter assay revealed that FXR activation inhibited th

211 Chromatin immunoprecipitation and luciferase reporter assays revealed a direct binding of NANOG to a

212 iCLIP and subsequent mRNA reporter assays revealed a function for Hnrnpa1 in the r

213 Antimir and luciferase reporter assays revealed a specific and direct effect of

214 Luciferase reporter assays revealed an allele-specific regulation o

215 Synthetic enhancer reporter assays revealed that Isl1 operates as an integr

216 Genome-wide transcriptome analysis and reporter assays revealed that NeoB specifically inhibits

217 Chromatin immunoprecipitation and reporter assays revealed that Notch1 intracellular domai

218 Reporter assays revealed that promoters from different a

219 Transgenic mouse reporter assays revealed that the in vivo activity patte

220 Reporter assays revealed that the inhibitory effect of C

221 ChIP analysis, followed by enhancer reporter assays, revealed that this effect was mediated

222 Furthermore, Luciferase reporter assay reveals that Honokiol modestly increased

223 ng site-directed mutagenesis and an NFkappaB reporter assay screen, we have identified several charge

224 is extension and, using in vitro and in vivo reporter assays, show they fall into two functional clas

225 expression of LANA in a luciferase promoter reporter assay showed reduced HLA-DRA promoter activity

226 tigation of the interacting proteins using a reporter assay showed that many of them modulate Gro-med

227 In addition, luciferase reporter assay showed that NFATc4 directly regulated the

228 A luciferase reporter assay showed that P. gingivalis increased the a

229 The luciferase reporter assay showed that the rs2494752 G allele signif

230 In support, MMP7 promoter-reporter assays showed greater transcriptional activity

231 quantitative polymerase chain reaction, and reporter assays showed suppressed activity of SV40 trans

232 me Atlas (TCGA), miRNA target prediction and reporter assays showed that miR-491-5p directly targets

233 Reporter assays showed that presence of wild-type, but n

234 Luciferase reporter assays showed that the HNF-1beta binding sites

235 Luciferase reporter assays showed that the most important anti-fibr

236 Assays of several Ctcf binding sites using reporter assays showed that their regulatory activity re

237 and N-(3-oxododecanoyl)-l-homoserine lactone reporter assays, showing that Fap fibrils pretreated wit

238 Luciferase reporter assay shows a decrease in the promoter activity

239 Using luciferase reporter assays, site-directed mutagenesis, ChIP assays,

240 activity of 69 TE subfamilies by luciferase reporter assays, spanning all major TE classes, and show

241 patients' primary fibroblasts and luciferase reporter assays strongly favor an upregulation of COL4A1

242 rs of both LINC00339 and CDC4 and luciferase reporter assays suggest the risk SNP rs12038474 is locat

243 Dual luciferase reporter assay suggested that miR-98 directly targets Ca

244 ent with the results of an IFN-beta promoter reporter assay suggesting that all tPs function to antag

245 hibitors show very different activity in our reporter assay, suggesting that such compounds may be us

246 Pase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated t

247 m additional studies using DNA damage repair reporter assays support a role of SALL4 in inhibiting th

248 of A. mellifera (AmTRP-R) in a heterologous reporter assay system to determine the activities of var

249 We present here a novel GFP-based reporter assay that can monitor SL1 trans-splicing in li

250 We developed a novel reporter assay that enabled identification of natural co

251 y quantitative fluorescence imaging and gene reporter assays that drug binding to FABP1 and FABP2 pro

252 roperties of BEN-solo proteins, we performed reporter assays that indicate that both Bsg25A and Elba2

253 characterization, typically achieved through reporter assays that test whether a sequence can increas

254 Here we developed a reporter assay to quantify editing, and used it to impro

255 Using high-throughput luciferase reporter assay to screen for KLF2 activators, we have id

256 apping, epigenomic profiling, and individual reporter assays to delineate potential causal variants.

257 ciated loci, we performed massively parallel reporter assays to screen candidate functional variants

258 e used mathematical modeling and single-cell reporter assays to show that miRNAs, in conjunction with

259 we used NF-kappaB promoter-driven luciferase reporter assays to test HARE-mediated intracellular sign

260 We then used in vivo reporter assays to test the tissue-specificity of these

261 In reporter assays, two new sites display decreased enhance

262 Intriguingly, luciferase reporter assays using deletion constructs of a proximal

263 ession of MITF and HINT1 as well as promoter reporter assays using GPNMB promoter constructs, we coul

264 s of GLI mutations were tested in luciferase reporter assays using HeLa or neuroblastoma cell lines.

265 Luciferase reporter assays using wild-type and mutant ET-1 3' untra

266 Cell-based luciferase reporter assays using wild-type and mutant transgenes re

267 Luciferase reporter assays validated rhesus macaque SIRT1 as a dire

268 L-36R complex was generated and a cell-based reporter assay was established to assess the signal tran

269 Finally, a 45-pathway reporter assay was performed in knockdown cells.

270 In a luciferase reporter assay we found a 75% decrease in activity of Ag

271 By chromatin immunoprecipitation and a reporter assay, we demonstrate that Notch1 binds at posi

272 EJ5- and DR-green fluorescent protein (GFP) reporter assay, we found that COH29 could inhibit nonhom

273 Using a yeast PKA reporter assay, we identified genes that influence PKA a

274 ombining computational modeling and the BiLC reporter assay, we identified several novel small-molecu

275 protein immunoprecipitation, and luciferase reporter assay, we investigated how rates of mRNA transl

276 sing cAMP measurements and a transcriptional reporter assay, we observed that several constrained ago

277 devoid of RNAP2 occupancy using a functional reporter assay, we performed cis-regulatory element sequ

278 Using a cell-based reporter assay, we screened C4a against a panel of both

279 etry, bioinformatics analysis and luciferase reporter assay, we showed that miR-K6-5p directly target

280 Using an in vivo Wnt GFP reporter assay, we verified the upregulation of Wnt sign

281 By employing luciferase reporter assays, we also identified two promoters potent

282 Using transgenic reporter assays, we confirm that these enhancer alleles

283 Using chromatin immunoprecipitation and reporter assays, we demonstrate that FOXO3a regulates it

284 Using an in silico screen combined with reporter assays, we discovered that a diverse range of m

285 tent in different guide strand segments with reporter assays, we establish that weak base pairing in

286 Using luciferase reporter assays, we found that miR-495 directly targeted

287 Through quantitative proteomics and reporter assays, we found that the UBE3A(T485A) protein

288 Using gene reporter assays, we observed binding of these miRs to sp

289 Here, using luciferase-based reporter assays, we provide evidence that NRP1 regulates

290 Using GUS and GFP reporter assays, we reveal their distinct or overlapping

291 Using primer extension analysis and reporter assays, we show the importance of the DPE in tr

292 Due to intrinsic limitations of heterologous reporter assays, we sought to develop a gene editing app

293 Luciferase reporter assays were used to confirm Notch1 target gene

294 oretic mobility shift assays, and luciferase reporter assays were used to demonstrate that ChREBP bin

295 n, lentiviral overexpression, and Luciferase reporter assays were used to gain insight into the mecha

296 nalyzed for transcriptional regulation using reporter assays where all tested regions exerted regulat

297 ncreased mRNA stability, as predicted by the reporter assay, while also markedly decreasing transcrip

298 2, and p53 pathways was compared by in vitro reporter assays with and without the pre-extraction of P

299 cription factor-binding motifs, and in vitro reporter assays with putative cis-regulatory elements.

300 In a dual-luciferase reporter assay, WNV NS1 significantly inhibited the acti