ライフサイエンスコーパス: residual activity

1 ompared to wild-type TPH ( approximately 5 % residual activity).

2 produced an effect on kinase activity (<10% residual activity).

3 ctions (AMRs) occurred without detectable C5 residual activity.

4 ter 30minat 75 degrees C it showed about 10% residual activity.

5 glutathione S-transferase to a limit of 60% residual activity.

6 nase-deficient c-Abl mutant still retained a residual activity.

7 a missense mutation and is likely to retain residual activity.

8 oter reporter constructs tested exerted only residual activity.

9 meric capping protein (CP) accounts for this residual activity.

10 utant CodY proteins with different levels of residual activity.

11 ed with free enzyme which showed only 30% of residual activity.

12 three months with retention of more than 80% residual activity.

13 he cryb mutation, implying that CRYb retains residual activity.

14 he heme is conserved, which accounts for the residual activity.

15 ent inactivation of GST pi to a limit of 25% residual activity.

16 to inactivity rather than to differences in residual activity.

17 o alanine gave mutants with approximately 2% residual activity.

18 s of function and mutations that leave a low residual activity.

19 ith late-onset phenotypes showed up to 2.15% residual activity.

20 ty of the complete enzyme but possessed its "residual" activity.

21 ve dose was calculated from administered and residual activities.

22 ately 50% first step activity, displays only residual activity (0.2%) in the production of squalene f

23 Red blood cell glucose uptake residual activity, a surrogate of haploinsufficiency, co

24 nd reusability with retention of 72% and 60% residual activity after 10 uses for the enzyme immobilis

25 at room temperature for several months (92% residual activity after 260 days storage) and demonstrat

26 screened for improved stability by measuring residual activity after incubation under conditions mimi

27 tability changes only weakly correlated with residual activity against UDP-galactose.

28 ine to glutamine mutant displays the highest residual activity and a pH profile similar to that of th

29 plicing, giving rise to isoforms that retain residual activity and contribute to therapeutic resistan

30 The low residual activity and high chemical stability of Nmoc-Gl

31 that Nmoc-Glu, indeed, has exceptionally low residual activity and high chemical stability.

32 E536Q and L175F mice had low levels of residual activity and milder phenotypes.

33 vement in reverse sulfurylase activity (<10% residual activity) and complete restoration of forward s

34 owever, the mutated enzymes still maintained residual activity, and both mutant enzymes still bound t

35 me mutations result in heterodimers with the residual activity anticipated from individual constituen

36 ned by the inter-FVIIIa subunit affinity and residual activity approaches a value consistent with thi

37 O), and this inhibits catalysis; and (d) the residual activity arises from either the (CO)nECO state

38 Cr3+(n1)-PEPCK retains 15% residual activity as compared to unmodified PEPCK and de

39 purification steps, we demonstrate that the residual activity associated with the purified covalent

40 CAP1 activation was also altered with marked residual activity at high Ca(2+), the effect increasing:

41 ese data also provide evidence for localized residual activity at the cortical level.

42 llow an a priori prediction of heterodimeric residual activity based solely on the in vitro activity

43 D, and 7 were patients with profound BD with residual activity below 10%.

44 ion in GM-CSF-stimulated glucose uptake with residual activity blocked by wortmannin.

45 he R133P-beta mutant E1 exhibits significant residual activity but is markedly less stable than the w

46 a stable enzyme-adenylate complex, although residual activity can be detected at high ATP concentrat

47 Residual activity can be explained because each of these

48 n the tga2 tga5 tga6 triple mutant, and this residual activity cannot be suppressed by SA.

49 eta gene with +33 mutation showed 25% to 35% residual activity compared with normal.

50 ng aspartate 283 to alanine (D283A) left 10% residual activity, contrary to a previous report, but co

51 In two cases, the H6Q and D24N proteins, the residual activities could be markedly stimulated by addi

52 acid-sphingomyelinase deficiency (i.e., <2% residual activity) demonstrate abnormal permeability bar

53 This residual activity depends on the C-box domain of Cdh1, b

54 This residual activity depends significantly on the linker re

55 , which yield a protein that may retain some residual activity, each NFRCD mutation is likely to give

56 he immobilized enzymes retained up to 75% of residual activity even after eight consecutive cycles of

57 ions affords a modified enzyme with only 14% residual activity even though NFM and NEM target the sam

58 Residual activities for complexes I, II, and IV in muscl

59 unction mutants; furthermore, the measurable residual activities for T289C, Y293C, and A301C (<or=20%

60 Kinetic and NMR assays detect significant residual activity for all of the mutants.

61 strate to that of wild type, but it only has residual activity for G:U and no detectable activity for

62 ently immobilized protein compared to 20% of residual activity for the adsorbed protein after 25 d st

63 h were adjusted by subtracting the estimated residual activity from the first (18)F-FDG injection.

64 fore the second injection was performed, and residual activity from this additional delayed scan was

65 for the BH4 responsiveness; the mutants show residual activity (>30% of WT) and display various kinet

66 s not known whether cytoplasmic APOBEC3G has residual activity in activated T cells, even though viri

67 oid lambdacyhalothrin demonstrated prolonged residual activity in bioassay tests but failed to contro

68 ial (CRIM SA) in the baculovirus system, the residual activity in both patients was primarily from th

69 Residual activity in competitive attractor networks with

70 in adult HSCs is catalytically inactive, its residual activity in Dnmt3a-null HSCs can drive some dif

71 DH dependent and inhibited by triclosan; the residual activity in the fabI mutant was also NADH depen

72 The residual activity in the mutant L-CPTIs was sensitive to

73 aphy for detection of distant metastases and residual activity in the neck following thyroidectomy.

74 tic depression may actually be driven by the residual activity in the visually deprived retina.

75 of RDH12, T49M and I51N, exhibit significant residual activity in vitro, but are unable to control re

76 s and in prothrombinase assays measuring FVa residual activity, in agreement with studies of purified

77 and pre-steady-state kinetic analyses of the residual activities indicated that the rate-limiting ste

78 ivates heparinase I while a H203D mutant has residual activity, indicating a role of this residue in

79 The residual activity is attributed either to mistranslation

80 n partial function, and we deduce that their residual activity is dependent on the level of avrRpm1 s

81 nalysis of candidate genes revealed that the residual activity is due to alkyl hydroperoxide reductas

82 The residual activity is not coupled to the proton pump, and

83 y of LC/A by approximately 600-fold, and the residual activity is not inhibited by ArgHX.

84 The basis for the residual activity is probably as a result of expression

85 Surprisingly, we found that this residual activity is sufficient for wild-type levels of

86 Residual activity likely results from the synthesis of a

87 Residual activity may involve CO from the solvent bindin

88 Some of this residual activity might result from a neighboring argini

89 importance of these phosphates explains the residual activity observed for mutants that lack key res

90 (ii) mTLL-1 is an in vivo pCP that provides residual activity observed in Bmp1(-/-) embryos.

91 st whether this domain might account for the residual activity observed in ExoT GAP mutants.

92 Instead, the diminished sensitivity of the residual activities of E305Q and D504N mutants, but not

93 e biosensor configuration, the enzyme gave a residual activity of 0.18% of the amount originally appl

94 choroid plexus was essentially ablated (i.e. residual activity of 10.9 and 3.9% at 5 and 30 min, resp

95 ong-term stability experiments show a better residual activity of 40% for the covalently immobilized

96 s include strategies aimed at increasing the residual activity of a missing enzyme (enzyme replacemen

97 alternative splicing of exon 2 modulates the residual activity of AMPD1 in individuals with this muta

98 Mn(II) supports residual activity of both the native and fluorinated enz

99 ization capacity, raising the possibility of residual activity of channels formed by TRPV6(D541A) pro

100 Residual activity of D195A is very low, V87A is not expr

101 susceptibility produced by mutations and the residual activity of drugs against resistant viruses.

102 Residual activity of K165M is insensitive to pyridoxal p

103 great interest, as Cosnier et al. reported a residual activity of only 0.33% of the amount initially

104 he total available protein to counteract any residual activity of prolyl hydroxylases.

105 Further, the residual activity of T229E-p70s6k was not affected by ra

106 a highly conserved active site residue, the residual activity of tBPA-labeled 2B4 observed in previo

107 We found that the residual activity of the alpha H44A luciferase was marke

108 the plasma is usually measured based on the residual activity of the C1s protease not involved in th

109 the predicted effect of the mutations on the residual activity of the DTDST protein.

110 The residual activity of the enzyme after GSNO treatment was

111 The initial rate vi represents the residual activity of the enzyme before inhibitor dissoci

112 A tailor-made ink was studied, and the residual activity of the enzyme was 85% after additives

113 entified receptor responsive to estrogen, or residual activity of the ERalpha splice variant reported

114 Residual activity of the gossypol-modified PLA2 from sev

115 vations and highlights the importance of the residual activity of the insecticide for this technique.

116 These results suggest that shutting down the residual activity of the limbic-arousal system might be

117 l, there was a close correlation between the residual activity of the mutant enzymes and the clinical

118 ntation experiments in yeast showed that the residual activity of the mutant proteins correlates with

119 ato carboxypeptidase inhibitor inhibited the residual activity of the mutants.

120 attenuated but not absent in mice with only residual activity of the phosphatase Src homology 2 doma

121 Thus, both patients depend upon residual activity of the S209L mutant to support low lev

122 otransduction cascade during the light step, residual activity of the transduction cascade after the

123 0-fold in kcat with no change in Km, and the residual activity of the unresolvable P1V/M1P2V mixture

124 still degrade longer peptide substrates via residual activity of their Z subunits.

125 We found that the residual activity of these GMMA is largely due to non-li

126 ies in the patient with IH may be due to the residual activity of this mutant in activating the Dlx2

127 ntents of different isoflavone forms and the residual activity of trypsin inhibitors.

128 to myosin and the irradiation eliminates the residual activity of unbound inhibitor molecules, azidob

129 We determined that, in general, the residual activity of ZMPSTE24 patient alleles correlates

130 roinjection of dead PKAc reflects either low residual activity or binding to regulatory subunits with

131 patial lowpass and highpass filters, and the residual activity patterns then analyzed by linear class

132 for most of the FFA efflux in E. coli, with residual activity provided by other resistance-nodulatio

133 ne of the two heterozygous mutations has any residual activity remaining.

134 taryl-CoA dehydrogenase exhibit 7% and 0.04% residual activity, respectively, with human electron-tra

135 Gln mutants exhibited less than 0.5 and 0.1% residual activity, respectively.

136 ressing wild-type or mutant COX10 (retaining residual activity) restored complex I to normal levels.

137 ly samples characterised by a pectinesterase residual activity showed a softening also after one mont

138 negatively charged substitution mutants with residual activity suggests that the introduction of addi

139 uster have a significantly greater impact on residual activity than would be predicted.

140 Single and double mutants show 20-60% residual activities that are not stimulated by the PDC c

141 dimers between two mutant CobT proteins with residual activity that is so low that the resulting CobT

142 h of isolating its minimal fragment that has residual activity (the "minichaperone" core) and then id

143 nism 1B form in which mutant proteins retain residual activity, the severity of albinism depends on t

144 cyclin/CDK complexes could leave sufficient residual activity to promote S phase, or S phase-promoti

145 to the formation of modified enzyme with 92% residual activity toward 1-chloro-2,4-dinitrobenzene and

146 CO2 revealed similar inhibitory behavior and residual activity under saturating [CO].

147 One patient with residual activity underwent immediate biopsy that reveal

148 issense mutations were expressed to quantify residual activity using a new assay.

149 However, a low level of residual activity was detected, suggesting that a second

150 inding of the two PNPO dimers, and with high residual activity was found in Groups (ii) and (iii).

151 When FVa residual activity was measured after long exposure to AP

152 Presence of 1 mutation with residual activity was necessary but not sufficient for a

153 However, 0.2-0.4% residual activity was observed for the H145A mutant of h

154 The highest residual activity was observed with the His(34) position

155 with Ala reduced activity by 99.95%, and the residual activity was relatively unaffected by diethyl p

156 However, some residual activity was seen, presumably as a result of ra

157 As a likely explanation of this residual activity, we showed that CTbeta2, a form of CT

158 nitrocatechol sulfate (pNCS), detectable ASA residual activity were observed in primary and SV40t fib

159 Significant differences in prolonged (residual) activities were also observed for these modifi

160 ss cobalamin, whereas mut- mutations exhibit residual activity when exposed to excess cobalamin.

161 and R332H polypeptides revealed significant residual activity, whereas reverse transcription-PCR and

162 e Glu-243 to Asp mutant had approximately 2% residual activity, whereas the Glu-243 to Ala or Gln mut

163 both PPi hydrolysis and H+ translocation and residual activities with decreased DCCD sensitivity.

164 . crassa ND1 intron, but retains substantial residual activity with the mt LSU intron, and conversely

165 g the mt LSU intron, but retains substantial residual activity with the ND1 intron.

166 Correlation of residual activity with the number of histidines modified

167 t = 19.9 +/- 1.1 min-1, while R166A has only residual activity, with a kcat = 0.012 +/- 0.001 min-1 a