ライフサイエンスコーパス: residue

1 mportance of the conserved DDE motif and a W residue.

2 by the phosphorylation of a single tyrosine residue.

3 site is 11/9 nucleotides downstream of the A residue.

4 ues (132 to 140), and Leu-122, a pore-gating residue.

5 trate that lysine-56 is the key nucleophilic residue.

6 en the ligand's alkyl chloride and a guanine residue.

7 h NS5A that was phosphorylated on a specific residue.

8 thophores, phosphorylates Plin6 on conserved residues.

9 efulness of PSSM-RT for encoding DNA-binding residues.

10 e two isozymes only differ at six amino acid residues.

11 perty and occurrence frequency of amino acid residues.

12 omplementary nucleotides via the substituted residues.

13 ant in human cells also require the diacidic residues.

14 cluster via three cysteine and one histidine residues.

15 bition by small peptides that mimic the loop residues.

16 mpeting structure that sequesters pseudoknot residues.

17 ue level reveals potentially key interacting residues.

18 tabilized by the hydrogen bonding of guanine residues.

19 ccounts for 90 of the toxin's 387 amino acid residues.

20 n of each of these two phosphorylated serine residues.

21 d topology and interactions between specific residues.

22 whereas the P3 position avoided hydrophilic residues.

23 ferase requiring only two conserved cysteine residues.

24 loop containing a conserved trio of aromatic residues.

25 ucose, xylose and 4-O-methylglucuronic acid -residues.

26 an Slo2.1 were each replaced by 15 different residues.

27 K is not palmitoylated, even on these shared residues.

28 ionships of evolutionary information between residues.

29 7-specific aromatic box composed of tyrosine residues.

30 o a peptide resolution of 5 to 20 amino acid residues.

31 In addition, we map a region of NEMO (residues 112-150) in its coiled-coil 1 domain that imped

32 secondary glioblastomas and primarily affect residue 132, which helps coordinate substrate binding.

33 the anesthetic-binding cavity, its flanking residues (132 to 140), and Leu-122, a pore-gating residu

34 e TPR-like repeats of the M-region extend to residue 137 and that residues 90-125 form a novel loop t

35 n particular in the peptide corresponding to residues 14-24, containing Arg-14 and Lys-17.

36 valuated by its phosphorylation at threonine residue 172 (AMPK-Thr(P)(172)).

37 dicate that the elimination of phenylalanine residue 211 or 213 abolishes the UQ-dependent activity,

38 ed for the selective determination of marker residue 22,23-dihydroavermectin B1a in fat, kidney, live

39 ld enhancement of the beta-hairpin formed by residues 27-31 and 33-38.

40 eceptor substrate-1 phosphorylated at serine residue 312 in neurons and oligodendrocytes in the putam

41 A short-linear interaction motif between residues 337-343 of AKAP79 is the sole PP2B-anchoring de

42 Mutant dl1015, with deletion of residues 338 to 347 in the C-terminal region, has been a

43 Five highly represented signature amino acid residues (37A, 95K, 224N, and 242N in the M1 protein and

44 An arginine-to-histidine replacement at residue 435 in the binding domain of IgG3 to FcRn increa

45 eactivity of mAbs whose epitope being within residues 44-65 against reconstituted HDL particles, indi

46 ly, a helix-disrupting mutation of W50R into residues 44-65 restored the immunoreactivity of mAbs who

47 obically prepared fragment of mouse viperin (residues 45-362) complexed with S-adenosylhomocysteine (

48 ystal structure of a complex containing Sac3 residues 60-550 that indicates that the TPR-like repeats

49 Here, we report that the conserved lysine residue 714 in the ErbB4 ICD undergoes SUMO modification

50 particular a tyrosine-to-alanine mutation at residue 76 (Y76A), were essential for infectious virus p

51 the M-region extend to residue 137 and that residues 90-125 form a novel loop that links Sac3 to Thp

52 hat, after addition of a single-terminal Cys residue, a CdtB homologue from cytolethal distending tox

53 ed Kdo (3-deoxy-d-manno-oct-2-ulosonic acid) residue added by a third GT module belonging to the rece

54 ontained charged amino acids not more than 3 residues after the anchor amino acid at POmega, which en

55 tructure demonstrated the majority of unique residues align along the outer helical ridge.

56 bridges activated binding unless the mutated residue also formed a salt bridge with GpIbalpha, in whi

57 by direct dynamics simulations for ethyl as residue and attributed to the dynamics of elimination re

58 , V, and As mobility in the actively treated residue and the beneficial effects of treatment extend p

59 tional analysis, we identified two glutamine residues and a beta-hairpin within this putative DNA-bin

60 (LSD1) demethylates at both of these lysine residues and has been shown to disrupt neuronal maturati

61 nded to investigate roles of additional H3.3 residues and has implications for use in other developme

62 g association between plasma organophosphate residues and HbA1c but no association with acetylcholine

63 Here, we show that the regulatory N-terminal residues and the EH domain keep the EHD2 dimer in an aut

64 es of the phosphoserine and phosphothreonine residues and the preference for a dianionic charge state

65 n N-capped d-tetrapeptide, a phosphotyrosine residue, and a diester or a diamide group, we find that,

66 ontains several conserved positively charged residues, and a portion of the active site shows structu

67 completely symmetrical, rich in Arg and Trp residues, and able to adopt a native RTD-1-like structur

68 uts, stocks and outputs of wastes, products, residues, and emissions is established and quantified.

69 Both mutations affected conserved residues, and R291Q is orthologous to R294, which is mut

70 We found that substituting all five basic residues, and seven UM-associated substitutions, in yeas

71 nesis of the PTB domain and the CH1 tyrosine residues, and successive substitution of these tyrosines

72 of Sac3 was originally thought to encompass residues approximately 250-550, we report here the 2.3A

73 These residues are centered on the CRIB region, with the compl

74 Only when all the contacts between these key residues are eventually formed can the protein reach the

75 Since the ten Cys residues are highly conserved in Meteorin and Cometin, i

76 that interactions between charged amino acid residues are important both to directly stabilize the A1

77 These residues are located in the membrane-proximal region of

78 ost exclusively where functionally important residues are located.

79 modified RNA constructs in which specific G residues are replaced with (th) G, an emissive isomorphi

80 Surprisingly, these residues are separated in primary sequence, but cluster

81 and protein-protein interactions because key residues are truncated.

82 isulfides between sequence-adjacent cysteine residues are very rare and rather startling structural f

83 ions show that the electric dipole moment of residues around heme a changes with the redox state, hen

84 k of an appropriately positioned active site residue as a catalytic base leads us to propose an atypi

85 oxylation of propionate 4, but with a lysine residue as an essential proton shuttle.

86 g of consecutive glucuronic acid 2-O-sulfate residues as selectively targeting HCII.

87 rgely results from the presence of an acidic residue at its N-terminal region.

88 The conserved Glu residue at position 5 (E5) of mature (pseudo)pilins is e

89 Mutation of a charged residue at the interface (Arg-103) weakens the interface

90 change even in the absence of a protonatable residue at the Lys-300 position.

91 ING and that it depends on the nature of the residue at this position.

92 so evoked a hyperphosphorylation of CTD Ser2 residues at 5' ends of genes that is conserved in yeast.

93 aPred_poc is able to rank putative catalytic residues at lower (better) ranked positions, which can f

94 nother by substituting the variable cysteine residues at position III in the protein.

95 , a 27-amino-acid tail that is rich in basic residues at the C-terminal end is responsible for the in

96 al features, in detergent and liposomes, for residues at the pore domain that agree with their locati

97 e incorporated deuterium content on a single residue basis increases the spatial resolution of this t

98 Mapping of conserved and nonconserved residues between K1-K10 and K5-K14 onto the structure de

99 Moreover, mutation of this residue blocks PKD2's interaction with Focal Adhesion Ki

100 of FisR through the three conserved cysteine residues (C53, C64 and C71), FisR activates the expressi

101 Mutations of these residues cause an 18,000-fold reduction in catalytic rat

102 TPs) selected from the library contained a 5-residue consensus motif, LRLLR in positions 5-9.

103 Some missense mutations changed essential residues conserved among Schizosaccharomyces species.

104 bind two b-type hemes through the histidine residues conserved between the MsrQ and NOX protein fami

105 From the analysis of residue contacts, we conclude that electrostatic interac

106 About 50% of these newly formed bound residues contained one nitrogen atom and had a molecular

107 A ladder of H-bond donating residues creates a 'polar track' demarking the ion-condu

108 Alanine scanning mutation of Epep revealed residues critical for Tbx3, Klf4 and Esrrb transcript re

109 To identify key residues crucial for MjAgo function, we analysed the eff

110 ation, a covalent bond is formed between VVD residue Cys108 and its cofactor flavin adenine dinucleot

111 removing single terminally-linked arabinose residues, decreased the extent of B cell activation.

112 ss, it is unlikely that the fossilized fecal residues depict year-round feeding habits.

113 inding poses and engage different subsets of residues, despite sharing a common morphinan scaffold.

114 yclic nucleotide-binding domain through a 37-residue domain and the HCN C terminus through the TPR do

115 of electrostatic forces on individual atoms, residues, domains and molecules, and generates an output

116 Second, a site approximately 20 residues downstream of this motif determines the size of

117 ces, we were able to highlight key conserved residues driving essential elements of TCR recognition.

118 he gating region of the pore, namely between residues E90, E123, D253, N258, and the protonated Schif

119 utations were localized near or at conserved residues essential for diiron ion coordination.

120 -1 interaction profile, while mutants with S residues exchanged at 255 and 279/282 did not.

121 However, mutants with S residues exchanged at positions 365, 368, and 373 exhibi

122 rils with the hydrophobic edge and histidine residues extending in an ordered array as the catalytic

123 Binding affinity is greatly enhanced by residues flanking the crystallographically-defined recog

124 onarily conserved human CDC73 N-terminal 111 residues form a globularly folded domain (hCDC73-NTD).

125 domain of FUS (FUS-LC), a segment of only 57 residues forms the fibril core, while other segments rem

126 More recently, new demands for ultralow-residue fruit have increased the adoption of mating disr

127 The high frequency of these residues generates short peptides, some of which are too

128 1) The central gate residue Glu(130) (Glu(90) in Chlamydomonas reinhardtii (

129 ibril structures indicate that key glutamate residues (Glu-31 and Glu-61) in these domains may be sit

130 Single-channel translocations of a 10-residue, guest-host peptide revealed that there were fou

131 Residues H141, N146, F147, and Y150 cluster at the predi

132 Insertion of a 9-residue hemagglutinin epitope in IS1S2, but not in IS5S6

133 Residues His154 and His216 were identified as essential

134 around the active site in domain A, and two residues (I476 and V482) were within coiled or beta-shee

135 ontained an extra repetitive-domain cysteine residue in 1Dx5 that was important for understanding the

136 The probe design exploits a lysyl residue in EGFP that is essential for chromophore matura

137 lation and acetylation, but the role of this residue in intrinsic KRAS function has not been well cha

138 ties are stimulated by AKT, or by mutating a residue in MRE11 that we show is phosphorylated by p70S6

139 argeting the only other known phosphorylated residue in the first FF domain.

140 between the ligand and a conserved aromatic residue in the ligand-binding pocket.

141 now identified a highly conserved glutamate residue in the transmembrane region of E. coli TatC, whi

142 ains to probe the function of the D1-Asn(87) residue in the water-oxidation mechanism.

143 By mutating residues in ABD1, we find that this activity is mediated

144 with phosphorylation of at least 11 distinct residues in all three caspase-9 domains by nine kinases.

145 t are presumed to require phosphorylation of residues in alpha1C and C-terminal proteolytic cleavage

146 flank the PBR, we evaluated the role of PBR residues in autopolysialylation and found that the requi

147 bout the influence of cooking on antibiotics residues in eggs are limited.

148 Most information about drug residues in eggs concern their concentrations in raw mat

149 of biosensors for the control of antibiotic residues in food are highlighted.

150 missense mutations affecting crucial lysine residues in histone H3 genes significantly contribute to

151 concerned about the presence of these drugs residues in honey as they often lead to health concerns

152 To evaluate the potential role of these residues in hydrophobic gating, Leu267 and Leu270 in hum

153 Importantly, we also find that none of these residues in isolation plays a key role in IAPP self-asse

154 etween water accessible and lipid accessible residues in membrane proteins.

155 e global and quantitative analysis of lysine residues in native biological systems.

156 uncovered the distribution of Gag correlated residues in specific protein surfaces of the inner face

157 by nitrosylation) permits positively charged residues in the C-terminal helix to engage in DNA bindin

158 By manipulating the highly conserved -GH- residues in the CGHC active site of PDI, we created PDI

159 Mutational studies reveal that all the five residues in the conserved sequence are required for bind

160 a scanning mutagenesis approach to identify residues in the extracellular loops and transmembrane se

161 and Arg(447) cooperate with other amino acid residues in the GERAMT-binding site for proper chaperone

162 als the presence of this motif with critical residues in the homolog of other Helicobacter gastric pa

163 Alanine substitutions of key residues in the interface support the functional signifi

164 otably by phosphorylation of several Ser/Thr residues in the N-terminal tail.

165 Key residues in the polymerase are located in similar positi

166 mized for interactions with subtype-specific residues in the PPARdelta ligand-binding domain (LBD).

167 constructed two RBDs mutated in multiple key residues in the receptor-binding motif (RBM) of RBD and

168 of an oligosaccharide to selected asparagine residues in the sequence N-X-S/T (X not equal P), a moti

169 ubstitutions from ClO2-labile to ClO2-stable residues in the viral proteins, which likely increased t

170 PheK reacted with adjacent Lys, Cys, and Tyr residues in thioredoxin in high yields.

171 residue to either C6-OH group of the glucose residues in Tre.

172 c mutated genes, frequency, mutation hotspot residues, in silico predictions, and functional assays w

173 extended the sampling times for large bulky residues, incorporated the modeling of glycans on the su

174 ed when the sequence begins with a beta-hGly residue instead of a (S)-beta-Caa(l) constituent.

175 termined that K74 and K76 were critical Ubc9 residues interacting with the negatively charged residue

176 e mass spectrometry, we precisely mapped key residues involved in substrate recognition and catalysis

177 ctures, the bulky and hydrophobic BP pyrenyl residue is entirely solvent-exposed in the major groove

178 Compelling evidence is found that the Tyr140 residue is involved in expelling the product from the bi

179 Because this residue is just before the gamma-secretase cleavage site

180 d agronomic factors underlying neonicotinoid residues is needed to inform evidence-based policy.

181 ds on the protonation state of the K-channel residue K362.

182 rus nucleoprotein (NP), including the lysine residues K77, K113 and K229.

183 tonated Schiff base (SBH), as well as nearby residues K93, T127, and C128, indicates that the equilib

184 From the 214-residue LC domain of FUS (FUS-LC), a segment of only 57

185 In addition, FPOP analysis at the residue level reveals potentially key interacting residu

186 n events ( approximately 8 degrees ) using a residue-level coarse-grained model of the ribosome.

187 of the glycan, with the reducing-end mannose residue ligated to Ca(2+) in a primary binding site and

188 53-19.01microg.kg(-1)) but below the maximum residue limit (100microg.kg(-1)).

189 ts were moreover much lower than the maximum residue limits (MRLs) required by the Commission Regulat

190 We found that the residues making up the folding nucleus tend to interact

191 introduced with the aim to improve hospital residues management.

192 ostshredder treatment of automobile shredder residue may increase the recovery of CMs from ELVs.

193 multiple PrP(C) segments containing Asn/Gln residues may act in concert along a replicative interfac

194 g of E128K IL-1beta, suggesting that the Lys residues mediate integrin binding.

195 of the MOB1/NDR2 complex and define key MOB1 residues mediating MOB1's differential binding to Hippo

196 d in vitro experiments highlighted potential residues mediating the AP-1/Arf1 interaction and the unl

197 In viruses with the N-terminal Cys residues mutated, TF is much less efficiently localized

198 Using a homology model, four residues (N251, A263, I299, and F387) were located in th

199 lation at an evolutionarily conserved serine residue near the carboxyl terminus (Ser-883 in Xenopus).

200 Hfq CTD transiently binds the basic Sm core residues necessary for RNA annealing.

201 detection of the formation of nonextractable residues (NER).

202 ng site and the nonreducing terminal mannose residue occupying an adjacent secondary site.

203 Ax1 gene corresponding to the extra cysteine residue of 1Dx5 was substituted by a cysteine codon thro

204 ogen-bonding interaction from the side chain residue of Asn315.

205 loop, through interactions between the last residue of post-M4 and Phe(170) of the conserved FPF seq

206 ssion or loss of phosphorylation at critical residues of different classes of specific transport prot

207 nsive alanine mutational analysis across 553 residues of E1E2 also resulted in identifying the epitop

208 eans with deleterious mutations at conserved residues of GmSACPD-C.

209 rmation of hydrogen bonds between C-terminal residues of lentil peptides and residues of the ACE cata

210 hat match the pharmacophore of the key actin residues of Pfn1-actin interaction and therefore have th

211 n though it lacks the TPR-binding C-terminal residues of Ssa1.

212 n C-terminal residues of lentil peptides and residues of the ACE catalytic site.

213 we were able to describe the motions of the residues of the beta-barrel as a collective rocking of l

214 dues interacting with the negatively charged residues of the NDSM.

215 ydrocarbon-stapled peptides that display key residues of the p53 transactivation domain have emerged

216 l external region aromatics with hydrophobic residues of the transmembrane domain, and contains the a

217 The aim of this study was to identify the residues of WRN involved in the binding and ATPase-drive

218 A serine residue on the DIV S2 helix was found to be sufficient t

219 vation of distinctive impact from individual residues on Dark and Light states.

220 ses (HDACs) remove acetyl groups from lysine residues on histone tails, promoting transcriptional rep

221 Charged residues on the LBD surface form pathways that facilitat

222 Accordingly, mutations in contact residues, or so-called "second shell" residues, that inc

223 ign cyclic homo-oligomers that employs a new residue-pair-transform method to assess the designabilit

224 e, this prefiltering increased the number of residue pairs (5% FDR) by 33% (n = 108 to n = 144).

225 CdiA-CTYkris activity, suggesting that these residues participate in substrate binding and/or catalys

226 cript, we have focused on OleTJE active site residues Phe(79), His(85), and Arg(245) to interrogate t

227 og responsible for the transfer of the amino-residue phosphoethanolamine (pEtN) to the lipid A of V.

228 ded complementarity-determining region 1beta residues present exclusively in the Vbeta8.1 segment med

229 Human apoE is a polymorphic 299-residue protein in which the less common E4 isoform diff

230 g human exposure to pesticides and pesticide residues (PRs) remains crucial for informing public heal

231 Here, we identified two conserved residues (R151, I155) in the syntaxin-1 linker region as

232 We show that substitutions of these residues reduce bulk translation initiation and diminish

233 EO water can be very effective in pesticide residue removal from fresh produce without affecting the

234 o RPA-binding motifs and ascertained the key residues required for these interactions.

235 meta-predictors, most of today's methods for residue-residue contact prediction are based entirely on

236 success in a number of areas such as protein residue-residue contact prediction, secondary structure

237 Extending this scaffold with suitable residues resulted in an interference with the kinase's R

238 ndent hydroxylation of a neighboring proline residue resulting in 40S ribosomal subunits that were bl

239 BA binding pocket in FePYR1 shows discrepant residues resulting in different binding affinity to ABA.

240 The two domains are connected by a 12-residue segment termed the stalk, which adopts a beta-st

241 site for monomer-monomer contacts, this six-residue sequence element also turns into a well-defined

242 fy a distinct C-terminal autoinhibitory four-residue sequence in CNAbeta1, (462)LAVP(465), which comp

243 rylatable (Ser to Ala, SA) or phosphomimetic residues (Ser to Glu, SE) reduced Brg1 phosphorylation b

244 ate, whereas double mutation of nonconserved residues (Ser/Thr(296/297)) may perturb the local fold.

245 the linear patterning of oppositely charged residues shows minimal variation.

246 Many of the 12-residue spontaneous membrane translocating peptides (SMT

247 We then made substitution mutants in DnaK residues suggested by the model to interact with Hsp90Ec

248 gy 2 (SH2) or SH3 domains or of the cysteine residue that undergoes LPS-induced palmitoylation.

249 ng unit was replaced with nonchelating polar residues that bridged over the Zn(2+) binding site and r

250 for catalysis of APOBEC3A, but pinpoints the residues that confer specificity towards CC/TC motifs.

251 We identified 13 TAS2R16 residues that contribute to ligand specificity and 38 re

252 By identifying the residues that establish plaque stability, these studies

253 rregularities in the disposition of adjacent residues that facilitate the specific binding of protein

254 s and humans, also contain multiple cysteine residues that form intra- and interprotamine sulfur-sulf

255 n particular, we identified several arginine residues that interact with the polyanionic substrate, a

256 proteolysis, as does leucine substitution of residues that overlap or are immediately upstream of the

257 roteins, like tau, are enriched with proline residues that regulate both secondary structure and aggr

258 quence preference for incision between two G residues that reside in a T-rich region of DNA.

259 ned and tested 22,660 mini-proteins of 37-43 residues that target influenza haemagglutinin and botuli

260 ontact residues, or so-called "second shell" residues, that increase affinity are typically identifie

261 harged DNA and positively charged amino acid residues, the translocation speed of DNA can be manipula

262 te the potential role of MT1-MMP cytoplasmic residue Thr(567) phosphorylation in regulation of metast

263 (LS) transfers regioselectively a fructosyl residue to either C6-OH group of the glucose residues in

264 addition to direct contributions of contact residues to binding affinity.

265 K) that can react with proximal nucleophilic residues to form intra- or intermolecular protein crossl

266 We mutated distinguishing active-site residues to generate enzymes that had a common Zn(2+) bi

267 Substitution of the Lys residues to Glu markedly reduced integrin binding of E12

268 reo-specifically oxidize actin's M44 and M47 residues to induce cellular F-actin disassembly.

269 ia to measure the propensities of amino acid residues to participate in helical secondary structure a

270 could reflect the contribution of microbial residues to soil N transformation.

271 the sulfur atom of their exclusive cysteine residues to the substrate.

272 nus as well as a strictly conserved arginine residue toward the C terminus of ORF52 play critical rol

273 k-in of the most commonly mutated amino acid residue, tyrosine 537, in the estrogen-responsive MCF7 b

274 We mutagenized the residue Val-136, which lines the anesthetic-binding cavi

275 beta2 integrin as a model, we found that the residue volume at the Phe position in the alpha1-helix i

276 se II (CaMKII) phosphorylation of RyR2-S2814 residue vs. normoglycaemia.

277 aracterization of mutant enzymes showed that residue W1065 is critical for the activity of GTF180-Del

278 es just 7-8 out of a total of 148 amino acid residues was clearly detected.

279 ritically, the positive charge of the lysine residues was necessary for fusion regulation, as alanine

280 , and site-directed mutagenesis of histidine residues, we demonstrated that MsrQ is able to bind two

281 C. sativa threshing residues were biorefined by consecutive supercritical ca

282 The reactivities of these His residues were correlated with solvent accessibility-rela

283 ne and intact histidine, lysine, or cysteine residues were discovered and characterized from high-mol

284 t effects on peptide retention for different residues were driven by the spatial orientation of trypt

285 the HA PCS (P2 position) avoided hydrophobic residues, whereas the P3 position avoided hydrophilic re

286 manner to create stretches of phosphorylated residues, which may be a means to amplify signals or sta

287 ex hydrogen bond network of four active site residues, which was installed in the late stages of labo

288 ormed by intermolecular stacking of aromatic residues, which would spatially constrain the keratin 1/

289 40S subunit and contacts mRNA via conserved residues whose functional importance was unknown.

290 that contribute to ligand specificity and 38 residues whose mutation eliminated signal transduction b

291 us substitution of four aromatic/hydrophobic residues with Ala dramatically impairs both IAPP self-as

292 roteomes and have identified several hundred residues with heightened reactivity that are enriched at

293 ssential redox-sensitive cysteine and lysine residues within N-terminus of channel protein.

294 This screen uncovered essential residues within Synaptotagmin that suggest a structural

295 bstrates, we found that mutation of specific residues within the exosite differentially affects MKK a

296 ns through covalent modification of cysteine residues within the RGS domain that are located distal t

297 anine was successfully incorporated into two residues within the transmembrane domain of KCNE1: F56 a

298 pecific mutational analyses of the conserved residues within WRDPLVDID indicated that Trp-637 plays a

299 ng of mAbs is the discrimination of isobaric residues (Xle): leucine (Leu) and isoleucine (Ile).

300 We also crystallized this five-residue zebrafish P450 17A1 mutant, and the active site