ライフサイエンスコーパス: restriction endonuclease

1 cognate recognition site for any particular restriction endonuclease.

2 he cleavage of cross-linked chromatin with a restriction endonuclease.

3 omic DNA being resistant to cleavage by SmaI restriction endonuclease.

4 e also more sensitive to DSBs generated by a restriction endonuclease.

5 ost, unmodified host DNA can be destroyed by restriction endonuclease.

6 re used to investigate DNA looping by NgoMIV restriction endonuclease.

7 ochondrial DNA binding protein and a type II restriction endonuclease.

8 actors and signal transducing proteins, of a restriction endonuclease.

9 I intron reverse transcriptase and a type II restriction endonuclease.

10 the recognition sequence of the NarI Type II restriction endonuclease.

11 and show it is a DNA modification-dependent restriction endonuclease.

12 ontain target sites for all natural type IIS restriction endonucleases.

13 nome from invading DNA through the action of restriction endonucleases.

14 rification of fragments after digestion with restriction endonucleases.

15 replication or resulting from the action of restriction endonucleases.

16 hen linearized by digestion with nicking and restriction endonucleases.

17 s in genomic DNA that had been digested with restriction endonucleases.

18 Escherichia coli topoisomerase I or against restriction endonucleases.

19 0 has amino acid sequence similarity to some restriction endonucleases.

20 pplied to the DNA binding of EcoRI and BamHI restriction endonucleases.

21 ge mechanism of AgeI is novel among Type IIP restriction endonucleases.

22 ty to DNase I and enhanced digestion by some restriction endonucleases.

23 od for genomic representation using Type IIB restriction endonucleases.

24 rmation about the methylation sensitivity of restriction endonucleases.

25 dy extends the range of mechanisms known for restriction endonucleases.

26 rmation about the methylation sensitivity of restriction endonucleases.

27 has not been observed hitherto among type II restriction endonucleases.

28 c domain related to the PD-(D/E)XK family of restriction endonucleases.

29 cificity and flanking sequence preference of restriction endonucleases.

30 g the uniform fragments produced by type IIB restriction endonucleases.

31 DNA indicate that SauUSI belongs to type IV restriction endonucleases, a group that includes EcoK Mc

32 s of kappa3' enhancer chromatin structure by restriction endonuclease accessibility and protein assoc

33 In vitro DNase I footprinting and restriction endonuclease accessibility assays reveal tha

34 tes in the bacterial genome before the toxic restriction endonuclease activity appears.

35 Traditionally, restriction endonuclease activity is assayed on simple s

36 I RM systems in vivo, but fail to block the restriction endonuclease activity of the archetypal Type

37 t mutation, but that its repair restores its restriction endonuclease activity.

38 dS for DNA sequence specificity and HsdR for restriction endonuclease activity.

39 Type IIP restriction endonuclease AgeI recognizes a palindromic s

40 marker (IS1245), as well as hsp65 and IS1311 restriction endonuclease analyses.

41 train of Clostridium difficile designated by restriction endonuclease analysis (REA) as group BI has

42 enome sequencing, phylogenetic analysis, and restriction endonuclease analysis (REA) comparisons with

43 gth polymorphism (RFLP) patterns obtained by restriction endonuclease analysis (REA) of an amplified

44 pulsed-field gel electrophoresis (PFGE), and restriction endonuclease analysis (REA) of whole-cell DN

45 ed that both PvuII ribotyping and HinfI/DdeI restriction endonuclease analysis (REA) show promise for

46 olates that had been previously genotyped by restriction endonuclease analysis (REA) to determine the

47 y screening an isolate collection of various restriction endonuclease analysis (REA) types.

48 - 2) days of cure of CDI were compared using restriction endonuclease analysis (REA) typing.

49 ith pulsed-field gel electrophoresis (PFGE), restriction endonuclease analysis (REA), and epidemiolog

50 ne sequence typing (slpAST), PCR-ribotyping, restriction endonuclease analysis (REA), multilocus sequ

51 Restriction endonuclease analysis (REA), PCR ribotyping,

52 rican pulsed-field electrophoresis 1 [NAP1]; restriction endonuclease analysis [REA] group BI).

53 The strain, which is restriction endonuclease analysis group BI, pulse-field

54 Initial studies employing methylation restriction endonuclease analysis provided evidence for

55 The isolates were characterized by restriction-endonuclease analysis (REA), pulsed-field ge

56 modification (R-M) systems that consist of a restriction endonuclease and a DNA methyltransferase.

57 primary pathway in which ClpXP degrades the restriction endonuclease and a mechanism dependent on th

58 onsisting of the DNA cleavage domain of BmrI restriction endonuclease and C.BclI, a controller protei

59 ally overlapping genes, eco29kIR, encoding a restriction endonuclease and eco29kIM, encoding methyltr

60 quency and type using the recently developed restriction endonuclease and postlabeling (REAP) assay.

61 in Escherichia coli was determined using the restriction endonuclease and postlabeling (REAP) method.

62 biological techniques, i.e., elution with a restriction endonuclease and signal and target amplifica

63 rovides a practical guideline for the use of restriction endonucleases and defines a fundamental prop

64 Additionally, uncharacterized restriction endonucleases and engineered variants presen

65 se that this Lys, which is conserved in many restriction endonucleases and is replaced by Glu or Gln

66 oning techniques, which eliminate the use of restriction endonucleases and ligase, have been widely u

67 e PD(D/E)XK superfamily (typified by type II restriction endonucleases and many recombination and rep

68 Dimeric restriction endonucleases and monomeric modification met

69 overlook the resolving power of well-chosen restriction endonucleases and often fail to report how t

70 Restriction endonucleases and other nucleic acid cleavin

71 Southern blot analysis using multiple restriction endonucleases and probed with multiple mtDNA

72 ophoresis genotyping utilizing two different restriction endonucleases and provided rapid results wit

73 abilities of the proteins to protect against restriction endonucleases and reductions in cytosine dea

74 mplex is reminiscent of the type IIE and IIF restriction endonucleases and the two systems may share

75 ment of homogeneous, fluorogenic polymerase, restriction endonuclease, and ligase assays based on the

76 tion site into the recognition sequence of a restriction endonuclease, and the use of a fluorogenic r

77 as (i) bisulfite treatment, (ii) cleavage by restriction endonucleases, and (iii) immuno/affinity rea

78 A combination of fluorescent primers, restriction endonucleases, and image analysis was used t

79 and sizes generated by the use of additional restriction endonucleases, and the maintenance of many d

80 pes by expressing a mitochondrially targeted restriction endonuclease, ApaLI, in cells of heteroplasm

81 Many restriction endonucleases are dimers that act symmetrica

82 Restriction endonucleases are highly specific in recogni

83 The type II restriction endonucleases are indispensible tools for mo

84 Type I restriction endonucleases are intriguing, multifunctiona

85 e demonstrate for the first time that type I restriction endonucleases are not stoichiometric but are

86 Fidelity indices for a large number of restriction endonucleases are reported here.

87 Restriction endonucleases are the basic tools of molecul

88 Restriction endonucleases are used prevalently in recomb

89 We employed the EcoRI restriction endonuclease as a model for the interaction

90 ectrophoresis (PFGE) of genomic DNA with the restriction endonucleases AseI, DraI, and XbaI, and we c

91 The modification-dependent restriction endonuclease AspBHI recognizes 5-methylcytos

92 n fragment length polymorphism patterns with restriction endonuclease assays (REA) using an amplified

93 to 4a, and also inhibits the activity of the restriction endonuclease BamH1 more efficiently than eit

94 d the scissile phosphate, as observed in the restriction endonucleases BamHI and BglI.

95 The application of a DpnI restriction endonuclease-based assay allowed the direct

96 Most restriction endonucleases bridge two target sites before

97 BtsCI belongs to a group of Type IIS restriction endonucleases, BsmI, Mva1269I and BsrI, that

98 The thermophilic restriction endonuclease BstYI recognizes and cleaves al

99 Type IIS restriction endonuclease BtsCI (GGATG 2/0) is a neoschiz

100 and defines a fundamental property by which restriction endonucleases can be characterized.

101 provides strong evidence that some type IIS restriction endonucleases carry two separate active site

102 Although all Type II restriction endonucleases catalyze phosphodiester bond h

103 The restriction endonuclease CglI from Corynebacterium gluta

104 Bacterial DNA is protected from restriction endonuclease cleavage by modifying the DNA u

105 ented DNA from sequenced genomes to quantify restriction endonuclease cleavage on a complex genomic D

106 hat modifies same sites protecting them from restriction endonuclease cleavage.

107 Type IIS restriction endonucleases cleave DNA outside their recog

108 BslI restriction endonuclease cleaves the symmetric sequence

109 he PvuRts1I-family of modification-dependent restriction endonucleases, cleaves deoxyribonucleic acid

110 SgrAI restriction endonuclease cooperatively interacts and cle

111 As a result, for the past 35 years type I restriction endonucleases could only be loosely classifi

112 gmrD (glucose-modified hydroxymethylcytosine restriction endonuclease) CT of pathogenic Escherichia c

113 ase chain reaction (PCR) primers so that the restriction endonuclease cuts immediately upstream of th

114 remodeling complex, monitored by exposure of restriction endonuclease cutting sites.

115 ant to DpnI digestion and sensitive to DpnII restriction endonuclease cutting.

116 nt amino acid sequence similarity to Type II restriction endonuclease CviJI that recognizes an overla

117 natural transformation, a markerless type II restriction endonuclease-deficient (REd) mutant was cons

118 A new Type III restriction endonuclease designated PstII has been purif

119 uated by polymerase chain reaction (PCR) and restriction endonuclease digest.

120 Restriction endonuclease-digested DNA exhibited a bubble

121 NA from infected cells, and analysing it via restriction endonuclease digestion and Southern blots.

122 sing an assay based on methylation-sensitive restriction endonuclease digestion of genomic DNA and 'h

123 lation ratios based on methylation-sensitive restriction endonuclease digestion of genomic DNA and 'h

124 Because restriction endonuclease digestion of genomic DNA is an

125 methotrexate was collected and genotyped by restriction endonuclease digestion or length polymorphis

126 Restriction endonuclease digestion was then used to iden

127 a published primer pair sequence followed by restriction endonuclease digestion with AvaI and HincII

128 A nomenclature is described for restriction endonucleases, DNA methyltransferases, homin

129 nclude non-specific DNA-degradation enzymes, restriction endonucleases, DNA-repair enzymes, resolvase

130 ur data show that the high efficiency of the restriction-endonuclease-DNA-polymerase (RE-pol) DNA syn

131 sequence identity to M.FokI, BtsCI and FokI restriction endonucleases do not share significant amino

132 e-3-phosphate dehydrogenase (GAPDH), and the restriction endonuclease DrdI from thermal inactivation

133 terminal subdomains of the homodimeric EcoRV restriction endonuclease each bear a net charge of +4 an

134 recognition sequences for type IA, IB and IC restriction endonucleases (EcoKI, EcoAI and EcoR124I, re

135 se, DNase I, and by the site-specific type I restriction endonuclease, EcoKI.

136 DNA cleavage by the type III restriction endonuclease EcoP1I was analysed on circular

137 The binding of the restriction endonuclease EcoRI to DNA is exceptionally s

138 ed with atomic resolution how a prototypical restriction endonuclease, EcoRI, binds to the DNA target

139 e sensitive to the induction of an exogenous restriction endonuclease, EcoRI, but not to UV irradiati

140 site I in the related blunt cutting type II restriction endonuclease EcoRV, as well as that found in

141 tures to two structurally similar 'PD-D/ExK' restriction endonucleases (EcoRV and HincII) that also g

142 Analysis of the gmrS/gmrD restriction endonuclease enzyme family and its IPI* fami

143 nd orphan MTases - those lacking the cognate restriction endonuclease - facilitate evolution of new p

144 The data obtained from three restriction endonuclease fingerprints, generated from ea

145 The restriction endonuclease fold [a three-layer alpha-beta

146 ypes of DNases belonging to HNH/EndoVII- and restriction endonuclease-fold, and RNases of the EndoU-l

147 ts with SmaI and HpaII methylation-sensitive restriction endonucleases followed by AP-PCR amplificati

148 rget populations of immobilized T cells with restriction endonucleases for downstream analysis.

149 se Picker) assists in the rational choice of restriction endonucleases for T-RFLP by finding sets of

150 The type II restriction endonucleases form one of the largest famili

151 Moreover, we discovered that removal of restriction endonucleases from donor bacteria resulted i

152 gments that resulted from digestion with the restriction endonucleases FseI, ApaI, SmaI, and AscI and

153 e conclude that iceA1 in CH4 is a functional restriction endonuclease gene, while iceA1 in 60190 is n

154 protein to the catalytic domain of the FokI restriction endonuclease, generating a BPV1 E2-FokI chim

155 is method provides a unique tool for cloning restriction endonuclease genes and has many other potent

156 Comparable selection involving imported restriction endonuclease genes is proposed for the regio

157 Unlike most restriction endonucleases harboring the same core fold,

158 Restriction endonucleases have proven to be especially r

159 The 2.8 A crystal structure of the type II restriction endonuclease HincII bound to Ca(2+) and cogn

160 crystal structure of the unliganded type II restriction endonuclease, HincII, is described.

161 Type I restriction endonuclease holoenzymes contain methylase (

162 ethylation of the sequence recognized by the restriction endonuclease HpaII (pCpCpGpG).

163 methylated and consequently impedes more the restriction endonuclease HpaII digestion process.

164 the electrode is subsequently treated with a restriction endonuclease HpaII which recognizes the 5'-C

165 digestion of DNA by a temperature-dependent restriction endonuclease in 320 nL.

166 he nonspecific cleavage domain from the FokI restriction endonuclease in a cloning vector of choice.

167 A limitation of type IIS restriction endonucleases in assembly of long DNA sequen

168 d the genes encoding all four active type II restriction endonucleases in H. pylori strain 26695 usin

169 d restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters

170 method for following the digestion of DNA by restriction endonucleases in real time without the use o

171 Most restriction endonucleases, including FokI, interact with

172 n activity and are relatively insensitive to restriction endonuclease-induced death.

173 Restriction endonucleases interact with DNA at specific

174 ening method was devised to convert type IIS restriction endonucleases into strand-specific nicking e

175 The FokI restriction endonuclease is a monomeric protein that rec

176 The primary target of SgrAI restriction endonuclease is a multiple sequence of the f

177 The SfiI restriction endonuclease is a tetramer in which two subu

178 Fragmentation by a methyl-sensitive restriction endonuclease is followed by size fractionati

179 ed a fluorescent marker using a mutant EcoRI restriction endonuclease (K249C) that enables prolonged,

180 he ultra-sensitive polymerase chain reaction/restriction endonuclease/ligase chain reaction mutation

181 Therefore, type I restriction endonucleases, like their type II counterpar

182 Finally, our method identifies a novel restriction endonuclease-like domain in the C-terminus o

183 in secondary structure, we identify nine new restriction endonuclease-like fold families among previo

184 The C-terminal catalytic domain has a restriction endonuclease-like fold.

185 these unknown families to a number of known restriction endonuclease-like structures and thus assign

186 ese pathways using as a model end joining of restriction endonuclease linearized plasmid DNA in whole

187 he active sites is inactivated, the type IIS restriction endonuclease may nick only one strand.

188 lowing digestion of the proximal region with restriction endonuclease MboI or RsaI, or both.

189 to the presence of the methylation-dependent restriction endonuclease McrBC in the bacterial host.

190 Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a c

191 ing genomic DNA with a methylation-sensitive restriction endonuclease (MSRE) and amplification of mul

192 of nlaIIIR, which encodes the CATG-specific restriction endonuclease NlaIII in Neisseria lactamica.

193 ttern of cleavage sites for methyl-sensitive restriction endonuclease not found in a nonexpressing si

194 to the action of the ATP-dependent type III restriction endonucleases on DNA.

195 tional activity and reduced accessibility to restriction endonucleases on the transient MMTV promoter

196 double-strand breaks (DSBs) with the I-SceI restriction endonuclease or after stalling or collapsing

197 mcomitans was linearized by digestion with a restriction endonuclease or when genomic DNA was used di

198 nsmission using either mitochondria-targeted restriction endonucleases or TALENs.

199 REPK (Restriction Endonuclease Picker) assists in the rational

200 wo gH gene variants, readily recognizable by restriction endonuclease polymorphism, are well conserve

201 basis for identifying gL and gO variants by restriction endonuclease polymorphism.

202 A restriction endonuclease protection assay was designed a

203 These were verified by restriction endonuclease protection assays and DNase I f

204 We used the combinatorial selection method restriction endonuclease protection, selection, and ampl

205 We used the combinatorial selection method restriction endonuclease protection, selection, and ampl

206 -DNA hybrid, albeit with a limited number of restriction endonucleases, provides a method whereby ind

207 se model in which a mitochondrially targeted restriction endonuclease (PstI) was expressed in skeleta

208 xpressing an inducible mitochondria-targeted restriction endonuclease (PstI).

209 We demonstrate that, like other Type III restriction endonuclease, PstII does not turnover such t

210 interest as the C protein regulates both the restriction endonuclease (R) gene and the methyltransfer

211 ce between methyltransferase (M) and cognate restriction endonuclease (R).

212 encoding the controller (C) protein and the restriction endonuclease (R).

213 ansferase (M.CglI and M.NgoAVII), a putative restriction endonuclease (R.CglI and R.NgoAVII, or R-pro

214 iction-modification (R-M) systems comprise a restriction endonuclease (REase) and a protective methyl

215 -modification (R-M) systems produce separate restriction endonuclease (REase) and methyltransferase (

216 In type II RM systems, where the restriction endonuclease (REase) and protective DNA meth

217 The methylation-dependent restriction endonuclease (REase) BisI (G(m5)C downward a

218 The first reported Type IV restriction endonuclease (REase) GmrSD consists of GmrS

219 Found in the ATCC 10987 strain, BceSI is a restriction endonuclease (REase) with the recognition an

220 nine Recognition and Restriction), a Type IV restriction endonuclease (REase), as instigator for this

221 or more diverse C-terminal domains, such as restriction endonuclease (REase), protein kinase, HNH en

222 specific cleavage of probe-target hybrids by restriction endonucleases (REase).

223 Type II restriction endonucleases (REases) are deoxyribonuclease

224 Restriction endonucleases (REases) are highly specific D

225 inues the series of Surveys and Summaries on restriction endonucleases (REases) begun this year in Nu

226 Restriction endonucleases (REases) of the recipient bact

227 Restriction endonucleases (REases) recognize and cleave

228 etic information systems (AEGIS), 24 type-II restriction endonucleases (REases) were challenged to di

229 Restriction endonucleases (REases) with 8-base specifici

230 2) and the cleavage domains of BmrI and FokI restriction endonucleases (REases).

231 rt phage 9 g DNA sensitivity to >200 Type II restriction endonucleases (REases).

232 associated with companion DNA site-specific (restriction) endonucleases (REases).

233 The EcoRV restriction endonuclease recognises palindromic GATATC s

234 mismatched PCR primers to create or remove a restriction endonuclease recognition site relative to th

235 ondrial DRMD reporter contains a unique KpnI restriction endonuclease recognition site that is not pr

236 esence of ambiguous bases within an expected restriction endonuclease recognition site which were not

237 s, adapter/linker sequences, insert-flanking restriction endonuclease recognition sites and polyA or

238 most commonly used when polymorphisms alter restriction endonuclease recognition sites.

239 We used mostly the Tsp509I restriction endonuclease (recognition sequence: decreasi

240 nition sequence: decreasing AATT), the TspRI restriction endonuclease (recognition sequence: NNCA(G/C

241 BsrDI and BtsI restriction endonucleases recognize and cleave double-st

242 The MspJI modification-dependent restriction endonuclease recognizes 5-methylcytosine or

243 The FokI restriction endonuclease recognizes an asymmetric DNA se

244 R.SwaI, a Type IIP restriction endonuclease, recognizes a palindromic eight

245 HinP1I, a type II restriction endonuclease, recognizes and cleaves a palin

246 ng mechanism as found for some other type II restriction endonuclease recognizing similarly degenerat

247 ntal aspects of the biochemistry of Type III restriction endonucleases remain unresolved despite bein

248 A type IIG restriction endonuclease, RM.BpuSI from Bacillus pumilus

249 The Type IIS restriction endonuclease SapI recognizes the DNA sequenc

250 vage of single DNA molecules by the two-site restriction endonuclease Sau3AI were measured with optic

251 stal structure of the 'rare cutting' type II restriction endonuclease SgrAI bound to cognate DNA is p

252 Many restriction endonucleases show relaxed sequence recognit

253 nted nicking endonuclease sites separated by restriction endonuclease site(s).

254 oding the marker viruses also contain unique restriction endonuclease sites flanking the capsid-codin

255 all of the others by a unique combination of restriction endonuclease sites in Cmu2.

256 ble to create hundreds of unique new type II restriction endonuclease specificities.

257 strain resistant to digestion by each of 14 restriction endonucleases studied.

258 Roles for Type I restriction endonuclease subunit dynamics in restriction

259 bstrate, in conjunction with strain-specific restriction endonucleases suggests a model of short-frag

260 Here, we directly demonstrate a delay in restriction endonuclease synthesis after transformation

261 nition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperativ

262 recorded by the input-responsive action of a restriction endonuclease that cleaves an ancillary repor

263 MspJI is a novel modification-dependent restriction endonuclease that cleaves at a fixed distanc

264 triction-modification (R-M) systems encode a restriction endonuclease that cleaves DNA at specific si

265 BsoBI is a thermophilic restriction endonuclease that cleaves the degenerate DNA

266 MmeI is an unusual type II restriction endonuclease that combines endonuclease and

267 es of DNA, we have engineered a new type IIS restriction endonuclease that combines the specificity o

268 SgrAI is a type II restriction endonuclease that cuts an unusually long rec

269 te 3' recessed ends and one 4-bp-recognizing restriction endonuclease that generates a blunt end.

270 EcoRII is a type IIE restriction endonuclease that interacts with two copies

271 PvuRts1I is a modification-dependent restriction endonuclease that recognizes 5-hydroxymethyl

272 FokI is a type IIS restriction endonuclease that recognizes the 5'-GGATG-3'

273 sented are readily applicable to all type II restriction endonucleases that cleave both strands of do

274 ormed by ICP8 are resistant to the action of restriction endonucleases that cleave outside of the are

275 ounterintuitive observations that the mutant restriction endonucleases that exhibit relaxed specifici

276 aneously digested with four 6-bp-recognizing restriction endonucleases that generate 3' recessed ends

277 Restriction endonucleases that must bind at two recognit

278 Here, we study restriction endonucleases that require interaction at tw

279 nucleases for T-RFLP by finding sets of four restriction endonucleases that together uniquely differe

280 Analysis of mutant genomic DNA using restriction endonucleases that were unable to cut methyl

281 ble function, including protein kinases, and restriction endonucleases--that were interrupted by ISs.

282 e that before the appearance of the Esp1396I restriction endonuclease the intracellular concentration

283 Despite similarities to both Type I and II restriction endonucleases, the CglI and NgoAVII enzymes

284 ound that, in the presence of a thermophilic restriction endonuclease, thermophilic DNA polymerase ef

285 t mixture was treated with genotype-specific restriction endonuclease to digest the dominant genotype

286 sed a mitochondria-targeted form of the ScaI restriction endonuclease to introduce DSBs in heteroplas

287 Oxidized oligonucleotides were cut by a restriction endonuclease to provide small strands and en

288 EzyAmp exploits the ability of some restriction endonucleases to cleave substrates containin

289 lly available DNA processing enzymes such as restriction endonucleases to detect single nucleotide po

290 tome is converted to cDNA and processed with restriction endonucleases to generate low-complexity poo

291 The ability of 223 Type II restriction endonucleases to hydrolyze RNA-DNA heterodup

292 assay that uses the nuclease activity of the restriction endonucleases to measure sensitively their s

293 The type II restriction endonuclease TseI recognizes the DNA target

294 icle an in vitro system for the selection of restriction endonucleases using artificial cells.

295 them permeable to small molecules, like the restriction endonuclease we use to sequence-specifically

296 approach with 16 hexanucleotide-recognizing restriction endonucleases, we found marked substructurin

297 change to that observed in the other type II restriction endonucleases where DNA bound and unliganded

298 We identify amino acid substitutions in a restriction endonuclease, which impair restriction allev

299 tructure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence speci

300 More than 80 type IIA/IIS restriction endonucleases with different recognition spe

301 tutions were introduced into BsmBI and BsmAI restriction endonucleases with similar recognition seque