ライフサイエンスコーパス: restriction enzyme

1 the nonspecific cleavage domain of the FokI restriction enzyme.

2 is gap are substrates for Alu I, a blunt end restriction enzyme.

3 igestion with DpnII but not against the DpnI restriction enzyme.

4 tracycline-controlled expression of the SacI restriction enzyme.

5 uced either by HO endonuclease or the I-SceI restriction enzyme.

6 d with methylation-sensitive and insensitive restriction enzymes.

7 still no structural information on Type III restriction enzymes.

8 complexes and also with discussion of type I restriction enzymes.

9 endonucleases to function analogously to DNA restriction enzymes.

10 uperior accuracy and without the need to use restriction enzymes.

11 re of DNA sequences within the nucleosome to restriction enzymes.

12 kb DNA ladders when digested with two common restriction enzymes.

13 single combined cluster analysis of multiple restriction enzymes.

14 not require any restriction sites or use of restriction enzymes.

15 sk that rarely can be achieved using type-II restriction enzymes.

16 milarities between this and other classes of restriction enzymes.

17 e breakage of the P-O3' bond, in common with restriction enzymes.

18 r conditions compare favourably with type II restriction enzymes.

19 ragments are generated using frequent cutter restriction enzymes.

20 e limited in resolution by their reliance on restriction enzymes.

21 repeat as demonstrated by an increase in the restriction enzyme accessibility and in the size of DNA

22 e further substantiated by the findings of a restriction enzyme accessibility assay and TSA-stimulate

23 as demonstrated by micrococcal nuclease and restriction enzyme accessibility assays.

24 easured by chromatin immunoprecipitation and restriction enzyme accessibility assays.

25 apping dynamics is associated with increased restriction enzyme accessibility of histone-bound DNA af

26 We have used FRET and restriction enzyme accessibility to study nucleosome dyn

27 Moreover, using restriction enzyme accessibility, we detect no changes i

28 n might explain how other complex multimeric restriction enzymes act.

29 tiplex assay was applied to the detection of restriction enzymes activities as well as base excision

30 Therefore, PstII has characteristic Type III restriction enzyme activity as exemplified by EcoPI or E

31 -molecule amplification; introduction of the restriction enzymes affected both the rate and the "fate

32 Type IIs restriction enzymes allow seamless assembly of synthons

33 Physical mapping of DNA with restriction enzymes allows for the characterization and

34 Restriction enzymes also enhanced integration events at

35 ion of the sequence-specific activity of the restriction enzyme Alu1.

36 he isolated DNA is suitable for digestion by restriction enzymes, amplification by PCR and Southern b

37 sulfite sequencing and methylation-sensitive restriction enzyme analyses.

38 which was confirmed by methylation-dependent restriction enzyme analyses.

39 Restriction enzyme analysis and genomic sequencing ident

40 , which interrogates DNA methylation via the restriction enzyme analysis of PCR-amplified bisulfite t

41 nd circulation periods were characterized by restriction enzyme analysis of viral DNA and select gene

42 n of methylation using methylation-sensitive restriction enzyme analysis or focused on single-copy ge

43 In contrast, FRET and restriction enzyme analysis reveal that only PTMs throug

44 e between 1997 and 2003 were genome typed by restriction enzyme analysis.

45 Briefly, genomic DNA is digested with a restriction enzyme and adapters are ligated.

46 Restriction enzyme and complete sequencing data demonstr

47 ique that involves cutting the genome with a restriction enzyme and isolating the targeted sequences.

48 representations from a methylation-sensitive restriction enzyme and its methylation-insensitive isosc

49 he chromatin is solubilized, digested with a restriction enzyme and ligated at low DNA concentration

50 y was used to test this model for the EcoRII restriction enzyme and provide direct visualization and

51 The HMW DNA is digested using an appropriate restriction enzyme and size-fractionated using pulsed-fi

52 The elongated genome is digested with a restriction enzyme and stained with the intercalating dy

53 which is greatly influenced by the choice of restriction enzyme and the frequency at which it can cut

54 n is structurally similar to classic Type II restriction enzymes and contains the endonuclease cataly

55 ecific factors, including selectivity of DNA restriction enzymes and fragmentation method, as well as

56 present a model for restriction by type III restriction enzymes and highlight the similarities betwe

57 going molecular cloning steps and the use of restriction enzymes and ligases necessary in other avail

58 bout recognition and cleavage sites for both restriction enzymes and methyltransferases as well as co

59 samples digested with methylation sensitive restriction enzymes and mock digested are then transform

60 ethod that depended on methylation-sensitive restriction enzymes and real-time RT-PCR.

61 A number of different combinations of restriction enzymes and selective primers were examined,

62 n other translocases such as type I/type III restriction enzymes and SF1/SF2 helicases.

63 This review traces the discovery of restriction enzymes and their continuing impact on molec

64 among the most important characteristics of restriction enzymes and their corresponding methylases,

65 odels put forward for the action of type III restriction enzymes and their inadequacies.

66 was first digested by methylation-sensitive restriction enzymes and then precipitated by methyl-bind

67 ins how ArdA can bind and inhibit the Type I restriction enzymes and we demonstrate that 6 different

68 r enzymes that change DNA supercoiling (e.g. restriction enzymes) and are suitable for use in a high-

69 and at high resolution the DSBs induced by a restriction enzyme, and we characterize their impact on

70 some structural insights into the working of restriction enzymes, and offers some preliminary data ne

71 tors of terminases, helicases, translocating restriction enzymes, and protein translocases possess a

72 se superfamily of proteins that include many restriction enzymes, and the structure of the active sit

73 plied to study both protein and nucleic acid restriction enzymes, and was demonstrated to accurately

74 s when we use data generated using different restriction enzymes, and when we reconstruct structures

75 s (PFGE) after digestion of genomic DNA with restriction enzyme ApaI.

76 the reduction in genomic complexity with the restriction enzymes approach, genotyping-by-sequencing.

77 The conclusion that type I restriction enzymes are catalytic relative to DNA has im

78 While many Type II restriction enzymes are dimers with a single DNA-binding

79 nded an observed association, using a custom restriction-enzyme assay to analyze the DNA in 158 sampl

80 y, using a combination of Sanger sequencing, restriction enzyme assays and targeted deep sequencing.

81 Here we describe the first restriction-enzyme-assisted LC-MS/MS sequencing study of

82 prototypical DNA-binding protein, the PvuII restriction enzyme, at microfluidic-encapsulated, DNA-mo

83 uplex is substituted by RNA we find that six restriction enzymes (AvaII, AvrII, BanI, HaeIII, HinfI a

84 ng prevents endonuclease activity by type II restriction enzymes BamHI, EcoRI and SalI, and inhibitio

85 The SfiI restriction enzyme binds to DNA as a tetramer holding tw

86 representation of every site of a particular restriction enzyme by short DNA tags.

87 The slow evolution and engineering of new restriction enzymes calls for alternative strategies to

88 The BcgI restriction enzyme can thus excise two DNA segments toge

89 native strategies to design novel and unique restriction enzymes capable of binding and digesting spe

90 e-wide analyses of DNA modifications rely on restriction enzymes capable of digesting genomic DNA at

91 ses are consistent with the observation that restriction enzymes caused extensive DNA cleavage in the

92 Mutant analysis by PCR and restriction enzyme cleavage (MAPREC) is used to measure

93 ble-strand breaks (DSBs) created in vitro by restriction enzyme cleavage in or near CGG*CCG or CTG*CA

94 ion sites can be highly biased by the use of restriction enzyme cleavage of genomic DNA, which is a l

95 hod, differences due to polymorphisms at the restriction enzyme cleavage sites are also observed betw

96 sensitive or fully resistant to isoschizomer restriction enzyme cleavage.

97 s available for the systematic evaluation of restriction enzyme combinations that can enrich for cert

98 Bisulfite Sequencing (RRBS) protocol to all restriction enzyme combinations.

99 eover, we show in silico that cuRRBS-defined restriction enzymes consistently out-perform MspI digest

100 or exposure of chromatin-sensitive sites to restriction enzymes could be used to detect this reorgan

101 Thus, germline expression of mitochondrial restriction enzymes creates a powerful selection and has

102 Zinc finger nucleases are artificial restriction enzymes, custom-designed to cleave a specifi

103 sing confocal Raman spectra, S1 nuclease and restriction enzymes demonstrated that the structural dif

104 Zinc finger nucleases (ZFNs) are engineered restriction enzymes designed to target specific DNA sequ

105 ysis with hemoglobin is demonstrated through restriction enzyme detection, and an enhancement in sens

106 were screened for the c.828+3A>T mutation by restriction-enzyme digest, single-strand conformational

107 ntional cloning requires the purification of restriction-enzyme-digested vectors prior to the ligatio

108 antitative PCR (qPCR) and glucosyl-sensitive restriction enzyme digestion (gRES-qPCR).

109 h no physical ends detected by sequencing or restriction enzyme digestion analysis, and lacked a cos

110 bisulfite sequencing, methylation sensitive restriction enzyme digestion and array-based detection t

111 Standard BioBrick assembly normally involves restriction enzyme digestion and ligation of two BioBric

112 (scCGI-seq), combining methylation-sensitive restriction enzyme digestion and multiple displacement a

113 on and adduct bypass and a slightly modified restriction enzyme digestion and post-labeling assays re

114 ntrol modules capable of mutation sensing by restriction enzyme digestion and real-time on-chip micro

115 d from an avian erythroleukemia cell line by restriction enzyme digestion and released from the nucle

116 A real-time RT-PCR pyrosequencing assay, a restriction enzyme digestion assay, and direct sequencin

117 using polymerase chain reaction followed by restriction enzyme digestion by investigators blinded to

118 for this hypothesis, we assessed, by using a restriction enzyme digestion coupled with LC-MS/MS metho

119 to pro-B cell lines and HS1 is accessible to restriction enzyme digestion exclusively in normal pro-B

120 Assessment of AMD variants was done by restriction enzyme digestion of PCR products and TaqMan

121 de polymorphism (SNP) assay based on PCR and restriction enzyme digestion or sequencing of the amplif

122 locations, provided that a suitable genomic restriction enzyme digestion profile is available.

123 onverted to an expression vector by a simple restriction enzyme digestion with MfeI (to "drop-out" th

124 ucts can be used directly for sequencing and restriction enzyme digestion without further purificatio

125 f the region encompassing the polymorphisms, restriction enzyme digestion, and detection of fragments

126 K, T, V, X, and U were characterized by PCR, restriction enzyme digestion, and/or sequencing.

127 The purified YAC DNA is suitable for restriction enzyme digestion, DNA sequencing and functio

128 enic epitope, as reported by diagnostic NciI restriction enzyme digestion, DNA sequencing, and wester

129 formation of the tetrahedron was verified by restriction enzyme digestion, Ferguson analysis, and ato

130 renal carcinoma cell lines was assessed with restriction enzyme digestion-coupled PCR and bisulfite g

131 according to their sequence similarities and restriction enzyme digestion.

132 as isothermal nucleic acid amplification and restriction enzyme digestion.

133 (or representation) of the genome following restriction enzyme digestion.

134 tients was performed by Sanger sequencing or restriction enzyme digestion.

135 parasites as determined by sequencing and/or restriction enzyme digestion.

136 RRBS involves restriction-enzyme digestion, bisulfite conversion and s

137 oint mutations uses nested PCR combined with restriction enzyme digestions, which is laborious and ti

138 comprehensive database of information about restriction enzymes, DNA methyltransferases and related

139 comprehensive database of information about restriction enzymes, DNA methyltransferases and related

140 The Type II restriction enzymes (e.g. EcoRI) gave rise to recombinan

141 e DNA-linked assembly of nanoparticles using restriction enzymes (e.g., MspI).

142 Our model system, restriction enzyme Ecl18kI, interacts with a FRET pair-l

143 Assaying the complex type I restriction enzyme, EcoKI, gives an activity footprint o

144 tic measurements: the cleavage of DNA by the restriction enzyme EcoRV.

145 All restriction enzymes examined are phosphodiesterases gene

146 After a short pulse of restriction enzyme expression followed by a long period

147 There are hundreds of restriction enzyme families but few are used to date, be

148 EcoP15I is the prototype of the Type III restriction enzyme family, composed of two modification

149 Digestion of chromatin with the restriction enzyme Fnu4HI reveals hypersensitive sites o

150 ific DNA nucleases produced by fusion of the restriction enzyme FokI endonuclease domain (FN) with th

151 TS-4 was analyzed with methylation-sensitive restriction enzymes, followed by polymerase chain reacti

152 Lastly, we show that a proper choice of restriction enzyme for the optical map may substantially

153 GB2.0 relies on the use of type IIS restriction enzymes for DNA assembly and proposes a modu

154 , prediction of nonsense-mediated decay, and restriction enzymes for RFLP analysis.

155 ots can be released by simple digestion with restriction enzymes for subsequent characterization by s

156 target, queries over 1.3 M independent NspI restriction enzyme fragments in the 200 bp to 1100 bp si

157 Here, we report a restriction enzyme-free approach to library generation u

158 this approach include facile amplification, restriction enzyme-free library generation, and a signif

159 d-type levels of homologous recombination at restriction enzyme-generated breaks but is deficient in

160 Active restriction enzyme genes have been identified, and their

161 t this is a reliable method to detect active restriction enzyme genes in newly sequenced genomes, the

162 ide containing the optimal substrate for the restriction enzyme GlaI.

163 ngle-insert cloning of DNA fragments without restriction enzymes has traditionally been achieved usin

164 Ionizing radiation and restriction enzymes have been shown to increase the freq

165 Restriction enzymes have proved to be invaluable for the

166 eened for this particular mutation using the restriction enzyme HhaI.

167 e developed HiCap, which combines a 4-cutter restriction enzyme Hi-C with sequence capture of promote

168 We verify our method by using restriction enzyme Hind III to cleave the fluorescent ds

169 e performed a previously reported assay, the restriction enzyme hotspot mutation assay (IgkappaREHMA)

170 e predigested with the methylation-sensitive restriction enzyme HpaII.

171 in structure of the MHRE exhibited increased restriction enzyme hypersensitivity and enhanced histone

172 Using methylation-sensitive restriction enzymes hypomethylated maternal sequences ar

173 and breaks (DSBs) induced by a site-specific restriction enzyme, I-SceI or by ionizing radiation (IR)

174 nerated by inducible expression of the AsiSI restriction enzyme in normal human fibroblasts.

175 To explore the possibility of using restriction enzymes in a synthetic biology based on arti

176 he expression of > 600 genes was affected by restriction enzymes in cells lacking PT, including induc

177 al mechanism of PT-dependent DNA cleavage by restriction enzymes in the face of partial PT modificati

178 , we report that both ionizing radiation and restriction enzymes increase the frequency of microhomol

179 We use two restriction enzymes independently to create a series of

180 Efficient cleavage of these 100-mers by a restriction enzyme indicates that the DNA adopts a nativ

181 A and render nucleosomal DNA accessible to a restriction enzyme, indicative of a chromatin-remodeling

182 y promote large-scale genomic deletions from restriction enzyme-induced DSBs; third, they are require

183 Indeed, we show that restriction-enzyme-induced double-strand breaks are suff

184 tion of the recognition sequence of a Type-I restriction enzyme is a complicated procedure.

185 (MSCC) with the HpaII methylation-sensitive restriction enzyme is a cost-effective method to pinpoin

186 four DNA fragments in the presence of eight restriction enzymes is demonstrated.

187 Restriction enzyme kinetic analysis suggests that these

188 Restriction enzyme KpnI is a HNH superfamily endonucleas

189 several algorithms and modules, updated the restriction enzyme library, added batch processing capab

190 eaction (PCR) and analyzed by sequencing and restriction enzyme mapping.

191 ed an artificial population consisting of 12 restriction enzyme marker mutants of Cucumber mosaic vir

192 lease) was identified as an inhibitor of the restriction enzyme McrBC.

193 In one mutant, the restriction enzyme-mediated integration cassette disrupt

194 A screen of restriction enzyme-mediated integration mutagenized cell

195 The procedure consists of three steps: a restriction enzyme-mediated ligation of an adapter to th

196 bisulfite conversion, methylation-sensitive restriction enzymes, methyl-binding proteins and anti-me

197 to introduce a recognition sequence for the restriction enzyme MlyI.

198 tation (MeDIP-seq) and methylation-sensitive restriction enzyme (MRE-seq) sequencing data to predict

199 ombined MeDIP-seq with methylation-sensitive restriction enzyme (MRE-seq) sequencing for comprehensiv

200 A is digested by the methylation-insensitive restriction enzyme MspI to generate short fragments that

201 age with a cocktail of methylation-sensitive restriction enzymes (MSREs).

202 ning the use of designed ZFNs and commercial restriction enzymes, multiple plant expression cassettes

203 No restriction enzymes, mutagenesis of internal restriction

204 A, cDNA is synthesized and digested with the restriction enzyme NlaIII.

205 While many restriction enzymes operate symmetrically at palindromic

206 Numerous techniques using either restriction enzyme or affinity-based approaches have bee

207 ted bacterial DNA, samples are fragmented by restriction enzymes or sonication, then thermocycled in

208 randed molecules of genomic DNA using either restriction enzymes or sonication.

209 es in buffer composition and the presence of restriction enzymes or specific nucleic acids.

210 he use of methylation-sensitive isoschizomer restriction enzyme pairs and/or sodium bisulfite treatme

211 e strongest cohemolytic reaction had similar restriction enzyme patterns.

212 hain reaction, bisulfite pyrosequencing, and restriction enzyme-polymerase chain reaction.

213 We have used a restriction enzyme protection, selection and amplificati

214 use a genetic reversion assay to show that a restriction enzyme, PspGI, protects cytosines within its

215 use model expressing a mitochondria-targeted restriction enzyme, PstI or mito-PstI, to damage mitocho

216 use model expressing a mitochondria-targeted restriction enzyme, PstI or mito-PstI.

217 t mapping method that combines 5hmC-specific restriction enzyme PvuRts1I with a 5hmC chemical labelin

218 ifies the ATP hydrolysis scheme for Type III restriction enzymes, questions remain as to why multiple

219 modification genes and an adjacent putative restriction enzyme (RE) operon likely form a restriction

220 lecular manipulation methods, we investigate restriction enzyme reactions with double-stranded (ds)DN

221 Type I restriction enzymes (REases) are large pentameric protei

222 e allele read frequency, strain specificity, restriction enzyme recognition site changes and flanking

223 ds have been extended to include fifteen new restriction enzyme recognition sites.

224 death by cleaving the E. coli genome at the restriction enzyme recognition sites.

225 on a user-defined template, (3) selection of restriction-enzyme recognition sites in the spacer betwe

226 DNA cleavage by the Type III restriction enzymes requires long-range protein communic

227 erases generating 3-OH and 5-P ends, but one restriction enzyme (restriction glycosylase) excises unm

228 complementary method, methylation-sensitive restriction enzyme sequencing (MRE-seq).

229 The restriction enzyme SfiI binds to DNA but leaves it intac

230 Restriction enzymes share little or no sequence homology

231 ation of chromosomal DNA with McrBC and DpnI restriction enzymes, single-stranded cDNA (ss-cDNA) liga

232 ed representation sequencing approach called Restriction Enzyme Site Comparative Analysis (RESCAN) to

233 nctions without constraints being imposed by restriction enzyme site location.

234 Each of four restriction enzyme site-tagged viruses was shown to be a

235 The ELAN method uses judicious choice of restriction enzyme sites coupled with simultaneous diges

236 plified by flanking primers that can include restriction enzyme sites for inserting the product into

237 rimarily occurs within two consecutive HpaII restriction enzyme sites in a tissue-specific manner, mo

238 ever, Clustal DNA alignments identified AFLP restriction enzyme sites that were undigested in the tis

239 To facilitate mutagenesis reactions, restriction enzyme sites were introduced in the tandem g

240 with single UV lesion surrounded by multiple restriction enzyme sites, we demonstrate in vitro that D

241 230 bp DNA segment containing five pairs of restriction enzyme sites, which can be used to produce a

242 f the cloned gmr genes in vivo and suggest a restriction enzyme specific for sugar modified HMC DNAs.

243 ification (RT-LAMP), and linear degradation (restriction enzymes) starting with hepatitis C viral RNA

244 0's DNA is resistant to cutting with several restriction enzymes, suggesting DNA modification, but de

245 sites including options for the selection of restriction enzyme suppliers, and (4) output files desig

246 ral and regulatory chromatin interactions by restriction enzyme targeting and two-step proximity liga

247 MmeI is an unusual Type II restriction enzyme that is useful for generating long se

248 TaqI is a 263-amino acid (aa) Type IIP restriction enzyme that recognizes and cleaves within th

249 Tth111II is a thermostable Type IIGS restriction enzyme that recognizes DNA sites CAARCA (R =

250 EcoP15I is a type III restriction enzyme that requires two recognition sites i

251 inc finger nucleases (ZFNs)-hybrid synthetic restriction enzymes that can be specifically designed to

252 SfiI is a member of a growing family of restriction enzymes that can bind and cleave two DNA sit

253 MspJI belongs to a family of restriction enzymes that cleave DNA containing 5-methylc

254 ctivity following BAC digestion with several restriction enzymes that cleaved different sets of genes

255 The product is digested by restriction enzymes that cut at unique sites between the

256 The restriction enzymes that cut DNA after interacting with

257 The standard mode of action for the restriction enzymes that excise their recognition sites

258 SfiI belongs to a family of restriction enzymes that function as tetramers, binding

259 al that BsrDI and BtsI belong to a family of restriction enzymes that possess two catalytic sites: a

260 The structure of AgeI is similar to the restriction enzymes that share in their target sites a c

261 loci encoding type IV methylation-dependent restriction enzymes that target DNA containing C5-methyl

262 ures offer a rare opportunity to compare two restriction enzymes that work on exactly the same DNA su

263 In contrast to restriction enzymes the Fenton reaction is known to clea

264 many recombinases, transcription factors and restriction enzymes, the importance of non-specific DNA

265 he BcgI endonuclease exemplifies a subset of restriction enzymes, the Type IIB class, which make two

266 age threshold for permeation of DNA bound to restriction enzymes through a nanopore that is associate

267 BRISK utilizes a Type IIB DNA restriction enzyme to create a defined representation of

268 ation of random fragmentation and a type III restriction enzyme to derive a densely covering sgRNA li

269 situ DNase Hi-C obviates the dependence on a restriction enzyme to digest chromatin, instead relying

270 PvuRts1I was the first identified restriction enzyme to exhibit specificity toward hmC ove

271 The program also finds the best restriction enzyme to further diversify HRM or FLP signa

272 me that expresses a mitochondrially targeted restriction enzyme to induce tissue-specific homoplasmy

273 Targeting a restriction enzyme to mitochondria in the germline compr

274 be combined with the nuclease domain of FokI restriction enzyme to produce TAL effector nucleases (TA

275 ave developed modification-dependent type IV restriction enzymes to defend the cell from T4-like infe

276 samples, reduces genome complexity by using restriction enzymes to divide the genome into fragments

277 ures of DGS include (1) use of high-frequent restriction enzymes to fractionate the genome into small

278 hich micrococcal nuclease is used instead of restriction enzymes to fragment chromatin, enabling nucl

279 We leverage codon degeneracy and type IIs restriction enzymes to generate orthogonal ligation link

280 method of genome reduction that employs two restriction enzymes to generate overhangs in opposite or

281 PEER uses adapters and class IIS restriction enzymes to generate tagged oligonucleotides

282 tion analysis, in which OTU DUBs are used as restriction enzymes to reveal linkage type and the relat

283 ubsequent restriction analysis with a single restriction enzyme, Tsp5091, yielded distinct RFLP patte

284 protein can be purified and forms an active restriction enzyme upon addition of restriction subunits

285 genomic DNAs treated with four endonuclease restriction enzymes using both chamber and droplet dPCR

286 The new class of modification-dependent restriction enzymes was named Type IV, as distinct from

287 In the presence of restriction enzymes, we directly visualize the cleavage

288 G2-C-G3-C-C recognition sequence of the NarI restriction enzyme were compared, using the oligodeoxynu

289 ease C gene of H. pylori and Sau-3 and Hha I restriction enzymes were used for polymerase chain react

290 When multiple restriction enzymes were used to map the transgene posit

291 Among this set are genes encoding restriction enzymes which, when active in E. coli, lead

292 esearch on zinc finger proteins and the FokI restriction enzyme (which revealed a bipartite structure

293 In this study, we use the EcoRII restriction enzyme, which employs a three-site binding m

294 ining numerous pores, allowing the access of restriction enzymes while preventing the DNA from physic

295 Engineering restriction enzymes with new sequence specificity has be

296 The creation of restriction enzymes with programmable DNA-binding and -c

297 analysis by analyzing the cleavage sites of restriction enzymes within exon 1 of the AR gene and fou

298 PFGE profiles were generated by use of six restriction enzymes (XbaI, BlnI, SpeI, SfiI, PacI, and N

299 roved T-vector system takes advantage of the restriction enzyme XcmI to generate a T-overhang after d

300 q to determine the frequency and spectrum of restriction-enzyme-, zinc-finger-nuclease-, and RAG-indu