ライフサイエンスコーパス: restriction fragment

1 athway were found to lie on a single 10.5-kb restriction fragment.

2 ith sample-specific linkers appended to each restriction fragment.

3 relies on the selective PCR amplification of restriction fragments.

4 ia at species and strain levels by resolving restriction fragments.

5 nt formation at meiosis by using single-dose restriction fragments.

6 termining the amplitude curve that describes restriction fragment amplitude as a function of mobility

7 used haplotype analysis, DNA sequencing, and restriction fragment analysis of mutations to evaluate t

8 in situ hybridization (FISH), flow-FISH, and restriction fragment analysis showed no change in telome

9 l copies was characterized from a chromosome restriction fragment and found to contain a sequence tha

10 Each EST detected a mean of 4.8 restriction fragments and 2.8 loci.

11 te DNA substrates, including several natural restriction fragments and different PCR-generated fragme

12 Detection of DNA restriction fragments and PCR product sizing is demonstr

13 mere length by Southern blot of the terminal restriction fragments and quantitative PCR (qPCR) of tel

14 to determine the relative positions of these restriction fragments and use them to serve as markers.

15 with one or two restriction enzymes and the restriction fragments are resolved by agarose gel electr

16 abeled with a different fluorescent dye, and restriction fragments are sized on a capillary DNA analy

17 These restriction fragments are stretched one by one in a micr

18 ical model for the location and shape of the restriction fragments as a function of fragment size, wi

19 We used the SC2-specific restriction fragments as templates to clone an allele of

20 membered ring pairs Im/Py and Hp/Py on a DNA restriction fragment at four 6-base pair recognition sit

21 Our method is applied to single-dose restriction fragment autotetraploid alfalfa data, and th

22 Phage 9 g restriction fragments can be degraded by DNA exonuclease

23 y of Chromosome III, affecting the size of a restriction fragment containing rDNA repeats and produci

24 od based on the partially circular nature of restriction fragments containing replication bubbles and

25 determined by computer-predicted lengths of restriction fragments containing the SNPs, and was furth

26 FM) has been used to image a 471-bp bent DNA restriction fragment derived from the M13 origin of repl

27 Of the 5154 restriction fragments detected by 882 ESTs, 2043 (loci)

28 We have used restriction fragment differential display for isolating

29 AR-beta2-mediated antitumor activity, we did restriction fragment differential display-PCR and cloned

30 a genetically related subgroup of bacteria, restriction fragment digest pattern (RDP) type III-3, su

31 an, except for STR102, which hybridized to a restriction fragment from G. latifolia.

32 The 199 bp restriction fragment has an apparent bend angle of 46 +/

33 (BAC) clones, sensitivity and specificity of restriction fragment identification exceeded 96% on rest

34 andLeader has been used to perform automated restriction fragment identification for more than 850,00

35 Telomeric restriction fragments in Magnaporthe isolates that infec

36 intermediates in 31 adjacent and overlapping restriction fragments in the spacer, ranging in size fro

37 Automated identification of restriction fragments involves several steps, including:

38 The sizing of restriction fragments is the chief analytical technique

39 From the terminal restriction fragment length (TRFL) distribution, the aut

40 cation protocol assays and telomere terminal restriction fragment length assays were performed on poo

41 lso find that in-nucleus ligation eliminates restriction fragment length bias found with in-solution

42 of the t-loops corresponded to the telomere restriction fragment length from the ALT cell lines as d

43 e to that of Towne-BAC, displayed an altered restriction fragment length pattern, and replicated with

44 ere, we describe inverse PCR-based amplified restriction fragment length polymorphism (iFLP), a new t

45 proach of spoligotyping with IS6110-targeted restriction fragment length polymorphism (IS6110-RFLP) a

46 ne M. tuberculosis strain using IS6110-based restriction fragment length polymorphism (IS6110-RFLP) a

47 lsed-field gel electrophoresis (PFGE), IS900 restriction fragment length polymorphism (IS900-RFLP), a

48 A novel method called "multiplex-terminal restriction fragment length polymorphism (M-TRFLP)" has

49 se chain reaction (qPCR), mutliplex-terminal restriction fragment length polymorphism (M-TRFLP), and

50 ree controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) meth

51 These data were used to develop a PCR-restriction fragment length polymorphism (PCR-RFLP) meth

52 gment by polymerase chain reaction and HinfI restriction fragment length polymorphism (PCR/RFLP).

53 and pfdhps genes were analyzed by LDR-FM and restriction fragment length polymorphism (RFLP) analyses

54 d voriconazole, and molecular relatedness by restriction fragment length polymorphism (RFLP) analysis

55 Restriction fragment length polymorphism (RFLP) analysis

56 tilocus sequence typing (MLST) and performed restriction fragment length polymorphism (RFLP) analysis

57 ng high-performance liquid chromatography or restriction fragment length polymorphism (RFLP) analysis

58 air, plasmids were sequenced or subjected to restriction fragment length polymorphism (RFLP) analysis

59 Restriction fragment length polymorphism (RFLP) analysis

60 In order to assess the -455G/A polymorphism, restriction fragment length polymorphism (RFLP) analysis

61 We used restriction fragment length polymorphism (RFLP) analysis

62 DNA dot blotting, IS1004 fingerprinting, and restriction fragment length polymorphism (RFLP) analysis

63 our nosocomial outbreaks were typed by Afut1 restriction fragment length polymorphism (RFLP) analysis

64 The isolates were genotyped by IS6110-based restriction fragment length polymorphism (RFLP) analysis

65 with culture-proven tuberculosis, combining restriction fragment length polymorphism (RFLP) analysis

66 pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism (RFLP) analysis

67 tuberculosis isolates were identified by PCR-restriction fragment length polymorphism (RFLP) analysis

68 Restriction fragment length polymorphism (RFLP) analysis

69 peats in the acidic repeat protein gene, (2) restriction fragment length polymorphism (RFLP) analysis

70 for Pneumocystis jirovecii that is based on restriction fragment length polymorphism (RFLP) analysis

71 This variation, revealed by restriction fragment length polymorphism (RFLP) and nucl

72 Conventional block PCR-restriction fragment length polymorphism (RFLP) and real

73 2007, 503 isolates were genotyped by IS6110 restriction fragment length polymorphism (RFLP) and spol

74 ts of the more commonly used methods, IS6110 restriction fragment length polymorphism (RFLP) and spol

75 eight eukaryotic species, and we describe a restriction fragment length polymorphism (RFLP) assay th

76 of the TNFB gene encoding TNF-beta and at a restriction fragment length polymorphism (RFLP) at posit

77 pressed sequence tag polymorphism (ESTP) and restriction fragment length polymorphism (RFLP) markers.

78 al evaluation of 479 samples was done with a restriction fragment length polymorphism (RFLP) method a

79 lts were also compared to an established PCR-restriction fragment length polymorphism (RFLP) method p

80 of 216 patients and all were genotyped using Restriction Fragment Length Polymorphism (RFLP) or seque

81 lates of a single serotype had a single wbiI restriction fragment length polymorphism (RFLP) pattern,

82 inks frequently demonstrate identical IS6110 restriction fragment length polymorphism (RFLP) patterns

83 The restriction fragment length polymorphism (RFLP) patterns

84 We identified unique HaeIII and HpaII gag restriction fragment length polymorphism (RFLP) profiles

85 An evaluation of the utility of IS6110-based restriction fragment length polymorphism (RFLP) typing c

86 The manual IS6110-based restriction fragment length polymorphism (RFLP) typing m

87 berculosis Hospital by spoligotyping, IS6110 restriction fragment length polymorphism (RFLP), and 24-

88 proportions to determine how pyrosequencing, restriction fragment length polymorphism (RFLP), and dir

89 al of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligo

90 y of herpes simplex virus (HSV) isolates use restriction fragment length polymorphism (RFLP).

91 ing 16S rRNA polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP).

92 in Francisella tularensis to type strains by restriction fragment length polymorphism (RFLP).

93 leotide sequencing of the plasmid DNA and/or restriction fragment length polymorphism (RFLP).

94 cer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) anal

95 Terminal restriction fragment length polymorphism (T-RFLP) analys

96 l transcribed spacer (ITS) database terminal restriction fragment length polymorphism (T-RFLP) approa

97 We used terminal restriction fragment length polymorphism (T-RFLP) of 18S

98 isotope probing (SIP) combined with terminal restriction fragment length polymorphism (T-RFLP), high-

99 munity shifts were characterized by terminal restriction fragment length polymorphism (T-RFLP).

100 The plaque was analyzed by terminal restriction fragment length polymorphism (t-RFLP).

101 We used terminal restriction fragment length polymorphism (TRFLP) analysi

102 ndent techniques, quantitative PCR, terminal restriction fragment length polymorphism (TRFLP) and nex

103 ield experiment were profiled using terminal restriction fragment length polymorphism (TRFLP) and seq

104 e nucleotide polymorphism (SNP) (screened by restriction fragment length polymorphism [RFLP] analysis

105 hout a region of the genome, demonstrating a restriction fragment length polymorphism among an encaps

106 n alternative to conventional nested PCR and restriction fragment length polymorphism analyses for th

107 Terminal restriction fragment length polymorphism analyses indica

108 ymes were used for polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-R

109 om 15 dogs and 27 cats were typed using URA5 restriction fragment length polymorphism analysis (RFLP)

110 subtyped with a small subunit rRNA-based PCR-restriction fragment length polymorphism analysis and a

111 e point, HCV genotype was determined by both restriction fragment length polymorphism analysis and ph

112 mmunity composition was assessed by terminal restriction fragment length polymorphism analysis and py

113 Restriction fragment length polymorphism analysis and RN

114 k of diarrhea in France were analyzed by PCR-restriction fragment length polymorphism analysis and se

115 Terminal restriction fragment length polymorphism analysis and se

116 mbrane protein A (ompA) gene sequencing, and restriction fragment length polymorphism analysis are cu

117 petitive units (MIRU-VNTR), and IS6110-based restriction fragment length polymorphism analysis cumula

118 morphism analysis, and sequencing as well as restriction fragment length polymorphism analysis for id

119 -based assays or a polymerase chain reaction-restriction fragment length polymorphism analysis in 160

120 were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis in 58

121 be homoplasmic by polymerase chain reaction/restriction fragment length polymorphism analysis in all

122 validated by polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis of 60

123 PCR-restriction fragment length polymorphism analysis of rib

124 s and performed DNA sequencing and PCR-based restriction fragment length polymorphism analysis of sev

125 s/genotype of isolates was determined by PCR restriction fragment length polymorphism analysis of the

126 p1a locus in the TIGR4 genetic background by restriction fragment length polymorphism analysis of the

127 It was confirmed to be genotype 1 by PCR-restriction fragment length polymorphism analysis of the

128 The assay agreed with PCR-restriction fragment length polymorphism analysis of the

129 PCR and restriction fragment length polymorphism analysis reconf

130 of Listeria monocytogenes by serotyping and restriction fragment length polymorphism analysis using

131 IS6110 restriction fragment length polymorphism analysis was pe

132 Restriction fragment length polymorphism analysis with a

133 , chromosome painting, Southern blotting and restriction fragment length polymorphism analysis, subcl

134 s using group-specific LGV real-time PCR and restriction fragment length polymorphism analysis.

135 ic PCR, followed by species-specific PCR and restriction fragment length polymorphism analysis.

136 s determined by direct PCR amplification and restriction fragment length polymorphism analysis.

137 olymerase chain reaction (PCR), or PCR-based restriction fragment length polymorphism analysis.

138 s the source of T. gondii infection based on restriction fragment length polymorphism analysis.

139 med by conventional methods and confirmed by restriction fragment length polymorphism analysis.

140 Isolates underwent IS6110-based restriction fragment length polymorphism analysis.

141 le nucleotide polymorphisms were detected by restriction fragment length polymorphism analysis.

142 were identified by polymerase chain reaction-restriction fragment length polymorphism analysis.

143 (38 of 59) of the IS6110 copies predicted by restriction fragment length polymorphism analysis.

144 ned by polymerase chain reaction followed by restriction fragment length polymorphism analysis.

145 d sequencing, and for RSTs by nested PCR and restriction fragment length polymorphism analysis.

146 Bacterial DNA was analyzed using terminal restriction fragment length polymorphism and 16S pyrotag

147 ere analyzed using polymerase chain reaction-restriction fragment length polymorphism and 5'-end [gam

148 multidrug-resistant tuberculosis cases using restriction fragment length polymorphism and by cross-ma

149 eumocystis control samples were genotyped by restriction fragment length polymorphism and multilocus

150 bovis strains had the same 16S ribosomal DNA restriction fragment length polymorphism and often had t

151 bs were analyzed and compared using terminal restriction fragment length polymorphism and sequence an

152 3, that had insertion sequence 6110 (IS6110) restriction fragment length polymorphism and spoligotype

153 We developed a terminal restriction fragment length polymorphism assay (TRFLP) f

154 sm in NQO1 using a polymerase chain reaction-restriction fragment length polymorphism assay and for t

155 r PCR, and/or a conventional PCR method (PCR-restriction fragment length polymorphism assay), were pe

156 ient isolate, there were two distinct IS1245 restriction fragment length polymorphism banding pattern

157 d 2008 from three humic lakes using terminal restriction fragment length polymorphism fingerprinting

158 throughput 18S rRNA tag sequencing, terminal restriction fragment length polymorphism fingerprinting,

159 were evaluated by polymerase chain reaction restriction fragment length polymorphism for polymorphis

160 We then studied the accuracy of restriction fragment length polymorphism for the -308 si

161 ed groups based on distinct ribosomal spacer restriction fragment length polymorphism genotypes (RSTs

162 s genotype classification was done by IS6110 restriction fragment length polymorphism genotyping and

163 as to identify the three markers by PCR and restriction fragment length polymorphism in parallel, an

164 o population studies, were examined by using restriction fragment length polymorphism IS6110 fingerpr

165 ative in discriminating the four-band IS6110 restriction fragment length polymorphism isolates from e

166 this approach with direct sequencing and the restriction fragment length polymorphism method indicate

167 We also identified a HindIII restriction fragment length polymorphism of F8A fragment

168 both parental alleles were demonstrated with restriction fragment length polymorphism of polymerase c

169 PCR-restriction fragment length polymorphism of small subuni

170 DNA sequencing and restriction fragment length polymorphism of the PCR prod

171 e and suitable for PCR checking, SNP typing (restriction fragment length polymorphism or amplificatio

172 techniques have been developed, based on PCR-restriction fragment length polymorphism or sequencing a

173 Its IS6110 restriction fragment length polymorphism pattern was ide

174 method for the 37% of isolates displaying a restriction fragment length polymorphism pattern with <6

175 Patients whose isolates had identical restriction fragment length polymorphism patterns and sp

176 of isolates of each subgroup share the same restriction fragment length polymorphism patterns of the

177 two Nocardia patient isolates showed unusual restriction fragment length polymorphism patterns with r

178 ipheral blood leukocytes and analyzed with a restriction fragment length polymorphism PCR method.

179 We devised a PCR-restriction fragment length polymorphism screen for the

180 ion, employing the polymerase chain reaction-restriction fragment length polymorphism technique (PCR-

181 We used polymerase chain reaction-restriction fragment length polymorphism to evaluate gen

182 virulence factors, ribosomal RNA gene spacer restriction fragment length polymorphism types (RSTs), o

183 jor histocompatibility complex class II DRB3 restriction fragment length polymorphism types 8/23, 3/1

184 For the SNPs, we designed a low-cost PCR-restriction fragment length polymorphism typing method.

185 IS6110 restriction fragment length polymorphism typing of cultu

186 found that 4 of 46 heterozygotes analyzed by restriction fragment length polymorphism were actually G

187 of nirS-denitrifers (assessed using terminal restriction fragment length polymorphism) was interactiv

188 n enzyme analysis of this gene (16S rRNA PCR-restriction fragment length polymorphism).

189 and identified, on the basis of biochemical, restriction fragment length polymorphism, and 16S rRNA g

190 -403, evaluated by polymerase chain reaction-restriction fragment length polymorphism, and CCR5Delta3

191 genes for toxins A, B and binary toxin using restriction fragment length polymorphism, and identifica

192 genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism, and IL-1RN var

193 enotypically matched (by spoligotype, IS6110 restriction fragment length polymorphism, and mycobacter

194 nalytical methods, including pyrosequencing, restriction fragment length polymorphism, and sequencing

195 g of the -174 nucleotide variant was done by restriction fragment length polymorphism, heteroduplex a

196 ermined on thyroid FNAB specimens by PCR and restriction fragment length polymorphism, plus direct se

197 By PCR-restriction fragment length polymorphism, sequence, and

198 Three molecular typing methods, IS6110 restriction fragment length polymorphism, spoligotyping,

199 rRNA bacterial community analyses (terminal restriction fragment length polymorphism, T-RFLP) were p

200 ntional methods based on cDNA sequencing and restriction fragment length polymorphism, the microarray

201 y the presence of the A1 allele of the TaqIA restriction fragment length polymorphism, which is assoc

202 nt type was assigned by a combination of PCR-restriction fragment length polymorphism-based assays.

203 263 controls underwent CD14 genotyping using restriction fragment length polymorphism-polymerase chai

204 Tissue typing was based on restriction fragment length polymorphism.

205 t mtDNA haplotyping by analysis with PCR and restriction fragment length polymorphism.

206 2+ ATPase gene (pfATP6) were assessed by PCR-restriction fragment length polymorphism.

207 were genotyped by polymerase chain reaction-restriction fragment length polymorphism.

208 were determined by polymerase chain reaction-restriction fragment length polymorphism.

209 nalyzed with 454 pyrosequencing and terminal restriction fragment length polymorphism.

210 C2 were analyzed by pyrosequencing or by PCR restriction fragment length polymorphism.

211 st change in community composition (Terminal Restriction Fragment Length Polymorphism; T-RFLP).

212 ological studies, mainly through identifying restriction fragment length polymorphisms (RFLP).

213 thamoeba isolates, mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) and cy

214 f N. veterana gave identical and distinctive restriction fragment length polymorphisms (RFLPs) for an

215 e often needed in order to create artificial restriction fragment length polymorphisms (RFLPs).

216 -phosphate dehydrogenase or to determine the restriction fragment length polymorphisms of X chromosom

217 genotyped for BsmI, ApaI, TaqI, and FokI VDR restriction fragment length polymorphisms were used for

218 Immunofixation, genomic restriction fragment length polymorphisms, and pulsed fi

219 DNA damage-associated markers, mean telomere restriction fragment length, and genomic stability diffe

220 Restriction fragment length-polymorphism analysis of vir

221 tion inferred from mitochondrial DNA (mtDNA) restriction-fragment length polymorphism (RFLP) data and

222 and diarrhea cases were compared by means of restriction-fragment length polymorphism (RFLP), rapid a

223 The Brazilian isolates were typed by restriction-fragment length polymorphism analysis and we

224 Restriction-fragment length polymorphism analysis of hum

225 tuberculosis strains associated with IS6110 restriction fragment-length polymorphism (RFLP) pattern

226 TB) during 1996-2001 were fingerprinted with restriction fragment-length polymorphism (RFLP).

227 Results of IS6110 restriction fragment-length polymorphism analyses were a

228 morphisms by using polymerase chain reaction-restriction fragment-length polymorphism analysis in 134

229 cribed sequence (ITS) and mitochondrial cox1 Restriction fragment-length polymorphism analysis of ITS

230 On the basis of a polymerase chain reaction-restriction fragment-length polymorphism analysis of the

231 By use of a polymerase chain reaction-based restriction fragment-length polymorphism analysis techni

232 We used spoligotyping, IS6110-based restriction fragment-length polymorphism analysis, and s

233 interaction between these variants using PCR/restriction fragment-length polymorphism assays in 454 s

234 Ninety maize restriction fragment-length polymorphism core markers we

235 representing 2 distinct ribosomal DNA spacer restriction fragment-length polymorphism genotypes (RSTs

236 Genotyping was performed by restriction fragment-length polymorphism polymerase chai

237 omegalovirus isolates were analyzed, both by restriction fragment-length polymorphism typing and by s

238 Restriction fragment-length polymorphism was used to det

239 aceae), was analysed by means of chloroplast restriction fragment-length polymorphism.

240 CFH, CFB, and 10q loci were screened by PCR-restriction fragment-length polymorphism.

241 enomic groups of C. burnetii are revealed by restriction fragment-length polymorphisms (RFLP).

242 ined by changes in polymerase chain reaction restriction fragment-length polymorphisms.

243 Isolates were genotyped using restriction-fragment-length polymorphism (RFLP) patterns

244 olates underwent insertion sequence (IS)6110 restriction-fragment-length polymorphism analysis, targe

245 sed sequence tags (ESTs) accounting for 2266 restriction fragments (loci) on the homoeologous group 3

246 We used pulsed-field gel electrophoresis and restriction fragment mapping to analyze the structure of

247 We used pulsed-field gel electrophoresis, restriction fragment mapping, and fluorescence microscop

248 Variations in melting behavior, observed as restriction fragment melting polymorphisms (RFMPs), were

249 measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent

250 ata lane analysis, a procedure for detecting restriction fragment multiplets while simultaneously det

251 e level of transcription, repair at the MspI restriction fragment of MET16 exhibits periodicity in li

252 is or a hybridization assay, in which a 3 Mb restriction fragment of the X chromosome is used as a ra

253 on (PCR) amplification and display of 3' end restriction fragments of double-stranded cDNAs was used.

254 o low-light tolerance by transformation with restriction fragments of genomic DNA of the pseudorevert

255 Analyses of restriction fragments of interest are performed by liqui

256 es between B DNA in BL21(DE3) and integrated restriction fragments of K-12 DNA inherited by REL606.

257 as measured by signal intensity of telomere restriction fragments on gels and fluorescence in situ h

258 ne of the markers is a polymorphic amplified restriction fragment (PARF) - a sequence found in both A

259 MLST housekeeping gene loci are detected by restriction fragment pattern analysis rather than sequen

260 RFLP analyses revealed different restriction fragment patterns between 50 healthy control

261 Restriction fragment patterns consisted of 14 to 18 frag

262 s, and services to plan and analyze terminal restriction fragment polymorphism (T-RFLP) experiments.

263 ployed to characterize bacteria and terminal restriction fragment polymorphism was used to profile bo

264 t frequent VVC based on profiles of terminal restriction fragment polymorphisms of 16S rRNA genes and

265 derived from Southern blots of the terminal restriction fragments (r > 0.96, P < 0.0001).

266 tion fragment identification exceeded 96% on restriction fragments ranging in size from 600 base pair

267 on the PCR amplification is the size of the restriction fragment, requiring a quantile normalization

268 ally significant, long-range interactions at restriction fragment resolution, assigning long-range in

269 are tools for the automated detection of DNA restriction fragments resolved on agarose fingerprinting

270 Analysis of the telomeric restriction fragments showed that DNA hybridizing to a (

271 with fundamental drawbacks, we used terminal restriction fragment (T-RF) length polymorphism profilin

272 The phylogenetic affiliation of terminal restriction fragments (T-RFs), together with correlation

273 mpared for a highly curved 199-basepair (bp) restriction fragment taken from the VP1 gene in Simian V

274 The electrophoretic mobility of a curved DNA restriction fragment taken from the VP1 gene in the SV40

275 A restriction fragment terminated by the DSB was isolated

276 DNA restriction fragments that are stably curved are usually

277 irtually pure and comprehensive libraries of restriction fragments that contained replication initiat

278 and found that SC2SC2 plants contained extra restriction fragments that hybridized to PaSLF17 in addi

279 Hence, DNA restriction fragments that migrate anomalously slowly in

280 These were contained in two restriction fragments that were cloned into the pUC19 po

281 lain the observed parameters of the terminal restriction fragment (TRF) dynamics while two-subpopulat

282 e limitations, we took advantage of terminal restriction fragment (TRF) length polymorphisms (T-RFLP)

283 lomere length, as expressed by mean terminal restriction fragment (TRF) length, was measured in 419 r

284 M genomewide map for mean leukocyte terminal-restriction fragment (TRF) lengths measured by Southern

285 The correlation of T/S ratios with Terminal Restriction Fragment (TRF) lengths measured by Southern

286 qPCR, and correlated with those of telomere restriction fragments (TRF) and quantitative FISH measur

287 individually, yielded the predicted terminal restriction fragments (TRFs) following digestion.

288 parameters using Southern blots of terminal restriction fragments (TRFs).

289 MLRFT resolved the 103 isolates into 15 restriction fragment types, giving a discrimination inde

290 ing; we refer to this approach as multilocus restriction fragment typing (MLRFT).

291 pulsed-field gel electrophoresis, multilocus restriction fragment typing, and multilocus sequence typ

292 rated by the selection of a subpopulation of restriction fragments using ligation-mediated PCR.

293 urification often involves the separation of restriction fragments via electrophoresis, the cutting o

294 In the present study, three amplified restriction fragments were identified in an esophageal a

295 photo-cross-linked in situ, and the telomere restriction fragments were purified by gel filtration ch

296 ei was photo-cross-linked, and the telomeric restriction fragments were purified by gel filtration.

297 BglII restriction fragments were size-selected to 500-600 bp,

298 eport the sequencing of the remainder of the restriction fragment, which revealed three further open

299 not only the size but also the order of the restriction fragments, which adds another dimension to t

300 ry of gapped DNA molecules by treating a DNA restriction fragment with the hydroxyl radical, generate