ライフサイエンスコーパス: restriction map

1 asis of their known locations on the genomic restriction map.

2 complete NotI and SalI, and a partial EcoRI restriction map.

3 genomic structure and generated a long-range restriction map.

4 e colinearity of the contig with the genomic restriction map.

5 ns by incorporating information from optical restriction maps.

6 34 bases, thereby yielding variable deduced restriction maps.

7 e for subcloning analysis and for generating restriction maps.

8 uorescein-AP antibody and thus determine the restriction maps.

9 quence of the gene was further subcloned and restriction mapped.

10 0 kb of D1S214 by YAC content and long-range restriction mapping.

11 e genes have been ordered by STS content and restriction mapping.

12 determined by polymerase chain reaction and restriction mapping.

13 data, and validation by whole-genome optical restriction mapping.

14 ion fragments without necessarily performing restriction mapping.

15 Chimerism of YACs was determined by FISH and restriction mapping.

16 raightening DNA molecules for use in optical restriction mapping.

17 Sequencing and restriction map analysis of the additional genomic clone

18 which also differ in chloroplast DNA (cpDNA) restriction map and nuclear ribosomal gene repeat phenot

19 Based on YAC restriction mapping and content analysis, DDX1 maps 340

20 Restriction mapping and DNA sequencing analyses provide

21 Restriction mapping and FISH confirmed the colinearity o

22 Restriction mapping and hybridization analysis of YAC an

23 Using detailed restriction mapping and partial DNA sequencing analysis,

24 Restriction mapping and partial sequence analysis of the

25 Restriction mapping and PCR analysis yielded a precise p

26 tions in ine were localized on cloned DNA by restriction mapping and restriction fragment length poly

27 Restriction mapping and RNA sequencing of these labeled

28 Restriction mapping and sequence analysis of recipient p

29 Restriction mapping and Southern blot analysis revealed

30 Restriction mapping and Southern blot analysis show that

31 Restriction mapping and Southern hybridization indicated

32 were constructed correctly as determined by restriction mapping and the absence of relevant protein

33 d that allowed the efficient construction of restriction maps and BAC contigs.

34 stic for normalizing alignment scores across restriction maps and demonstrate its utility for selecti

35 ility, biochemical tests, ribotyping, genome restriction mapping, and multilocus sequence typing (MLS

36 Partial sequencing, restriction mapping, and PCR analysis revealed that the

37 ficial intelligence techniques for automated restriction mapping, and use them to create a powerful m

38 The clone and restriction maps are in excellent agreement with maps ge

39 in which a coordinated set of single-enzyme restriction maps are simultaneously constructed.

40 Ordered restriction maps (BamHI and NheI) derived from single mo

41 A simple and rapid strategy for restriction mapping based on sequence-specific triple-he

42 Restriction maps based on five enzymes and sequence anal

43 Individual cosmid clones have been restriction mapped by directly imaging, with the atomic

44 es at defined intervals, and high-resolution restriction maps can be assembled from ensembles of sing

45 Restriction maps constructed for 23 cloned knob DNA frag

46 we reasoned that a high-resolution, ordered restriction map covering the entire genome could serve a

47 This procedure allows the construction of restriction maps covering several hundred kilobases and

48 ig depth of 15 molecules and high-resolution restriction maps covering the entire chromosome.

49 By using restriction mapping, DNA hybridization, sequencing, and

50 ongoing sequencing project, two whole-genome restriction maps (EcoRI and HindIII) of R. sphaeroides s

51 eady bacterial clone contig and a long-range restriction map for a 1-Mb interval spanning the deletio

52 A restriction map for a given restriction enzyme was gener

53 optical mapping, a system for making ordered restriction maps from ensembles of individual DNA molecu

54 New mapping approaches construct ordered restriction maps from fluorescence microscope images of

55 ata, automatically producing high-resolution restriction maps from images of individual DNA molecules

56 bly problem is actually more complex because restriction maps from single molecules are constructed,

57 Sequencing and restriction mapping have revealed that this plasmid, cp1

58 Restriction mapping indicated that the six clones repres

59 Such high-resolution restriction maps may become an indispensable resource fo

60 In this paper we describe the assembly and restriction map of a 1.05-Mb cosmid contig spanning the

61 t creates a high-resolution, in situ ordered restriction map of a bacterial genome.

62 versions, compared to an in silico (virtual) restriction map of a known genome sequence.

63 A whole-genome restriction map of Deinococcus radiodurans, a radiation-

64 The restriction map of DI6 will facilitate future mapping of

65 We have generated a high-resolution restriction map of nearly 500 kb spanning the MYCN locus

66 A long-range restriction map of the 2-Mb region that allowed estimati

67 s not depend on the prior determination of a restriction map of the bacterial chromosome but is based

68 s in relation to one another, resulting in a restriction map of the chromosome.

69 cific probes to interrogate a detailed EcoRI restriction map of the region, ZNF genes were found to b

70 n the clones, resulting in a high-resolution restriction map of the region.

71 The restriction map of the YAC contains three NotI sites.

72 mapping), which is a high-resolution ordered restriction mapping of a bacterial genome, is a relative

73 The restriction mapping of a massive number of individual DN

74 reside approximately 200 to 300 bp apart by restriction mapping of cloned genomic regions associated

75 We have performed restriction mapping of DNA molecules using restriction e

76 oughput genome-sequence data, and long-range restriction mapping of genomic and cloned DNA by pulsed-

77 f the exons of the methyltransferase gene by restriction mapping of long PCR fragments.

78 DNA sequence analysis and restriction mapping of one particular stretch of tandeml

79 Restriction mapping of the BAC contig placed the maximum

80 Restriction mapping of the different UGPase-cDNAs with B

81 Restriction mapping of the products amplified from HMC a

82 lotting, nucleotide sequencing, and detailed restriction mapping of the vbs-containing genomic clones

83 e used Optical Mapping to create genome-wide restriction maps of a complete hydatidiform mole and thr

84 Detailed restriction maps of microbial genomes are a valuable res

85 ecule restriction maps (Rmaps) and in silico restriction maps of sequence contigs to a reference.

86 to determine the exon/intron boundaries and restriction maps of the nearly contiguous structure of t

87 the construction of high-resolution ordered restriction maps of whole genomes from single DNA molecu

88 interactions is demonstrated by noncatalytic restriction mapping on a 4-kb DNA with three restriction

89 This method is potentially useful for restriction mapping other large DNA clones such as artif

90 Restriction maps provide landmark sequences at defined i

91 ysical map is consistent with the long-range restriction map reported previously.

92 Sequence and optical restriction mapping revealed 24 diploid nuclear chromoso

93 ', for the alignment of both single molecule restriction maps (Rmaps) and in silico restriction maps

94 ughput applications such as finger-printing, restriction mapping, +/- screening, and genotyping.

95 Since whole-genome optical maps are ordered restriction maps, sequenced strains of Shigella flexneri

96 Restriction mapping, sequencing, and comparison to the r

97 r before collecting DNA for analysis by PCR, restriction mapping, sequencing, or Southern.

98 ned into target DNAs of up to 50 kb in size, restriction mapping showed insertion to be relatively ra

99 Restriction mapping showed that these mutants span a gen

100 Additional restriction mapping shows alphaIIb is linked to the eryt

101 from human genomic DNA and characterized by restriction mapping, Southern blot analysis, and nucleot

102 Restriction mapping, Southern blot and PCR analysis of a

103 By restriction mapping, Southern blot, and sequence analyse

104 g M6a and M6b and have characterized them by restriction mapping, Southern hybridization with cDNA pr

105 ibe the assembly of a 1-Mb cosmid contig and restriction map spanning the candidate region for Finnis

106 Sequencing and restriction mapping suggest that the BACs may partially

107 Restriction mapping suggested that telomere-proximal rRN

108 determined by sequence analysis and detailed restriction mapping that G21, previously isolated as a '

109 For restriction mapping, the recombinant cosmid DNA is diges

110 se them to create a powerful multiple-enzyme restriction mapping tool.

111 sequence organization of this region, a 5-Mb restriction map was constructed based on YAC clones and

112 s homozygous deletion physically, long-range restriction mapping was performed using yeast artificial

113 By restriction mapping, we assembled a 110 kb genomic conti

114 Guided by endonuclease restriction maps, we chose PCR primer pairs that approxi

115 cleavage is an "Achilles' heel" approach to restriction mapping whereby a RecA-protein-oligodeoxynuc

116 it is often difficult to construct detailed restriction maps, with large number of restriction fragm