ライフサイエンスコーパス: restriction site

1 odification (e.g. the addition or removal of restriction sites).

2 and catalytic activity and abrogates a DraI restriction site.

3 model for a DNA decamer containing the EcoRI restriction site.

4 he opposite strand just outside of the PvuII restriction site.

5 ible for the presence or absence of the PstI restriction site.

6 ve; all were due to point mutations within a restriction site.

7 d by O6-methylguanine, positioned within the restriction site.

8 DR method requires the introduction of a new restriction site.

9 vivo compared with individuals lacking this restriction site.

10 ase I-SceI that recognizes and cuts an 18-bp restriction site.

11 cific bases in a sequence and generate a new restriction site.

12 indexing sequence located juxtaposed to the restriction site.

13 -2, which causes a gain or loss of an NlaIII restriction site.

14 ragments that do not have a common or unique restriction site.

15 l tags that can be derived from a predefined restriction site.

16 nding to the presence or absence of a single restriction site.

17 108, 310 and 350pb), due to a conserved BsaI restriction site.

18 281 bp) due to a 16 S rDNA conserved Hinf I restriction site.

19 npredicted HinfI RFLP, resulting in an EcoRI restriction site.

20 wed absence of the conserved 16 S rDNA HinfI restriction site.

21 ctinomycin (Spc) and causes loss of one HpaI restriction site.

22 nto unit-length genomes when cut at a unique restriction site.

23 istribution of ApaI conformations around the restriction site.

24 y cut lambda-phage DNA molecules at specific restriction sites.

25 ed a duplicated sgp region flanked by marker restriction sites.

26 h A and D genomes but differently flanked by restriction sites.

27 tifies genomes encoding systems with similar restriction sites.

28 to digestion since it does not contain these restriction sites.

29 ion-PCR followed by digestion at polymorphic restriction sites.

30 ng recombinant molecules without reliance on restriction sites.

31 lity of the different nucleotides within the restriction sites.

32 into the PCR product to protect most of the restriction sites.

33 gene construction in the absence of suitable restriction sites.

34 ithout the limitation of naturally occurring restriction sites.

35 such a family uses a specific combination of restriction sites.

36 on the creation of novel (SfiI) and (BstUI) restriction sites.

37 5-hmC at single base pair resolution at MspI restriction sites.

38 d SVs with or without creating or destroying restriction sites.

39 enic region that contains many commonly used restriction sites.

40 d by the limited availability of appropriate restriction sites.

41 a coli without dependence on suitably placed restriction sites.

42 le changes typically involve the revision of restriction sites.

43 re facilitated by a repeated set of flanking restriction sites.

44 vectors multiple cloning site with 10 unique restriction sites, (4) an oriT for conjugation-mediated

45 ions do not always occur within pre-existing restriction sites, a generalized PCR/RE/LDR method requi

46 solubility' mutations) flanked by convenient restriction sites, a translational enhancer, and a conse

47 ds and an antagonist showed that increase in restriction site accessibility correlates with transcrip

48 RA markedly increased restriction site accessibility within the promoter, incl

49 romatin structure, as revealed by changes in restriction site accessibility.

50 fficient algorithm to minimize the number of restriction sites against sets of cutter sequences, and

51 parative macrorestriction mapping using rare-restriction-site alleles (made with the Tn10dRCP2 family

52 The unique restriction sites allowed the insertion of test sequence

53 ated in order to evenly distribute 25 unique restriction sites along the length of the gene while ret

54 WGM utilizes restriction site analysis but arranges 200 to 500 bands

55 A chloroplast DNA (cpDNA) restriction site analysis of Argyranthemum (Asteraceae:

56 oduplex analysis, nucleotide sequencing, and restriction site analysis.

57 ng the sequence complementary to the cleaved restriction site and a 4-nucleotide 'indexing sequence.'

58 Several polymorphisms, including a BsmI restriction site and a poly(A) microsatellite, can be us

59 causes skipping of exon 5, deleting an RsaI restriction site and decreasing the amounts of mRNA foun

60 Both mutations disrupted a Taq I restriction site and restriction fragment length polymor

61 the oligonucleotide and the template at the restriction site and sufficient complementarity overall

62 I was exchanged for that of mBE I at a BspHI restriction site and was purified to homogeneity and cha

63 that now contains the NotI, AscI, and EcoRV restriction sites and allows the rapid cloning of NotI-E

64 This family of vectors has compatible restriction sites and modular design, so that the protei

65 In addition, for each possible target site, restriction sites and primer candidates are visualized,

66 sing appropriate combinations of polymorphic restriction sites and probes.

67 promoter type, restriction enzyme, number of restriction sites and with a noted exception, cell line.

68 e chain reaction primers containing internal restriction sites, and cloned into the phagemid pCOMB3H

69 d by the distribution of naturally occurring restriction sites, and requires minimal DNA sequencing.

70 3A26 hybrids were generated using convenient restriction sites, and site-directed mutagenesis was use

71 sequences lying adjacent to a given Type IIS restriction site are ligated between two DNA adapters.

72 Due to the reduced size, more unique restriction sites are available in the MCS, thus allowin

73 chromosome size, gene order, and some of the restriction sites are conserved between the two phage gr

74 loped a novel physical assay in which HaeIII restriction sites are positioned within the telomeric tr

75 Restriction sites are significantly underrepresented in

76 The spacer sequences between the nicking and restriction sites are tailored to create ss extensions o

77 ipts containing fewer than two of the chosen restriction sites are undetectable by cDNA-RDA.

78 It was concluded that point mutations at restriction sites are unlikely to account for the restri

79 tes containing the same lesion and strategic restriction sites around the lesion.

80 ion sites, whereas R recognizes unmethylated restriction sites as non-self and introduces a double-st

81 We therefore used restriction site associated DNA (RAD) sequencing to accu

82 Restriction site associated DNA (RAD) tags are a genome-

83 ion sequencing data were generated using the restriction-site associated DNA (RAD-seq) method.

84 We used restriction-site associated DNA sequencing (RAD-seq) to

85 Using double-digest restriction-site associated DNA sequencing, we surveyed

86 nation of flow cytometry and high-throughput restriction-site associated DNA tag sequencing, we show

87 , and eastern) via analysis of double digest restriction-site associated DNA using Next Generation Se

88 We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method

89 l wing-pattern variants, we used genome-wide restriction site-associated DNA (RAD) genotyping, tradit

90 First, we report our use of restriction site-associated DNA (RAD) polymorphism marke

91 By using restriction site-associated DNA (RAD) sequencing we were

92 e strategies include whole-genome, exome and restriction site-associated DNA (RAD) sequencing.

93 gle-nucleotide variants (SNVs) by sequencing restriction site-associated DNA (RAD) tags in 138 Micant

94 gle-nucleotide variants (SNVs) by sequencing restriction site-associated DNA (RAD) tags in 138 Micant

95 We use quantitative trait loci analysis of restriction site-associated DNA -tag single nucleotide p

96 Restriction site-associated DNA sequencing (RAD-seq) all

97 sity genetic maps of Zoysia japonica using a restriction site-associated DNA sequencing (RAD-Seq) app

98 ed out a genome-wide association study using restriction site-associated DNA sequencing (RAD-seq) of

99 types from more than 1,000 individuals using restriction site-associated DNA sequencing (RAD-seq).

100 High-throughput techniques based on restriction site-associated DNA sequencing (RADseq) are

101 Restriction site-associated DNA sequencing (RADseq) can

102 Large genomic data sets generated with restriction site-associated DNA sequencing (RADseq), in

103 This application of restriction site-associated DNA tags (RAD tags) reveals

104 h as pooled barcoded amplicon sequencing and restriction site-associated DNA tags, should be used to

105 Neurospora, we have adapted microarray-based restriction-site-associated DNA (RAD) mapping for use wi

106 t of RILs from a dense set of semicodominant restriction-site-associated DNA (RAD) markers and use si

107 Then, twenty of the offspring were used for Restriction-site-Associated DNA Sequencing (RAD-Seq) to

108 Here, we use genomic-scale restriction-site-associated DNA sequencing (RADseq) data

109 We employed restriction-site-associated DNA sequencing and integrate

110 Restriction-site-associated genomic markers are a powerf

111 of 31 mayfly (Ephemeroptera) communities and restriction-site-associated-DNA sequencing in the four m

112 imers to artificially introduce or abolish a restriction site at the target SNP site.

113 lion base pairs in length and used to insert restriction sites at any given nucleotide in a microbial

114 This module contains unique restriction sites at approximately 80 base pair interval

115 Methylation of CpG residues at restriction sites at the 5' end of both genes on the mur

116 yeast; however, this approach is limited by restriction-site availability.

117 i and find that, in agreement with levels of restriction site avoidance, EcoRI, but not EcoRV, cleave

118 An assessment of the SmaI restriction site-based multiplex PCR (SmaI-multiplex PCR

119 enoviral expression vector containing unique restriction sites between the human CMV-IE promoter and

120 Alleles which differ in size or restriction sites between these two strains were found f

121 amplifying DNA sequences flanking infrequent restriction sites by PCR and used the method to produce

122 to this vector by introduction of compatible restriction sites by PCR.

123 Artificial restriction sites can be introduced in known DNA sequenc

124 35-fold-repetitive B1 locus identified three restriction sites capable of discriminating type I (mous

125 morphism (SNP) variations in and around SmaI-restriction sites (CCCGGG).

126 Using a combination of haplogroup-specific restriction site changes and control region nucleotide s

127 , including very high G+C content and enzyme restriction sites characteristic of an HTF-island, no TA

128 ed, six of which were identical for numerous restriction site characters to plastome types found amon

129 Chloroplast DNA restriction-site comparisons were made among 24 species

130 s approach facilitates cloning regardless of restriction site compatibility and overcomes an importan

131 r such that annealing becomes independent of restriction site compatibility.

132 ged constructs with no undesirable vector or restriction-site-derived amino acids added to the expres

133 lution has failed to eliminate many possible restriction sites despite selective pressure, thus motiv

134 laboratory CMV strains that contained unique restriction sites developed for this purpose.

135 A amplification products resolved diagnostic restriction site differences for I. fulva and I. hexagon

136 126 strains were obtained at 16 polymorphic restriction sites distributed on nine PCR fragments.

137 d random incorporation of linkers containing restriction sites during ligation.

138 uction was verified by detection of a unique restriction site engineered into the SHRV genome between

139 The template has appropriate restriction sites engineered into it that can be digeste

140 I should facilitate the automation of cosmid restriction site fingerprinting needed for large-scale m

141 smid reverts mostly by +1 frameshifts in the restriction sites flanking the insert.

142 Unique eight base cutter restriction sites flanking the promoters and poly-adenyl

143 By mapping millions of restriction site-flanking reads back to the Escherichia

144 by the salt-bridges, does not seem to be the restriction site for a continuous water passage.

145 ng (a) a finely spaced DNA ladder carrying a restriction site for BamHI, (b) RNA translocation by DEx

146 orted, was 2988 A or C that introduces a new restriction site for Eco0109 I.

147 gle alpha-factor repeat was constructed with restriction sites for cassette mutagenesis flanking the

148 A borders without compromising the choice of restriction sites for cloning, since the pGreen cloning

149 , and, in some cases, identifying endogenous restriction sites for downstream molecular genetic appli

150 ion was performed using primers that amplify restriction sites for MspAI (A2 polymorphism-CYP17) and

151 onsensus sequence upstream of the undigested restriction sites for possible methyltransferase recogni

152 by PCR using primers containing appropriate restriction sites for subcloning into pTETnirB, which co

153 However, because the plasmid lacks restriction sites for the enzymes BlnI and I-CeuI, it wa

154 s quantitated as a function of the number of restriction sites for the restriction enzymes carried by

155 oxynucleotide complex protects an individual restriction site from methylation, thus limiting subsequ

156 y EcoRI and DraI enzymes at their respective restriction sites, G downward arrow AATTC and TTT downwa

157 s DNA hydrolysis by BstZ17 I at the enzyme's restriction site, GTA downward arrowTAC.

158 triction enzyme digestion whether any of the restriction sites have been altered.

159 Several restriction sites have been placed to flank the cassette

160 hetic vector arms from which the undesirable restriction sites have been removed by point mutations.

161 on of a silent mutation that created an XhoI restriction site in exon 7.

162 d mutation, TER1-Bcl, which generates a BclI restriction site in newly synthesized telomeric repeats,

163 g a single-base change that creates a unique restriction site in one of a pair of alleles.

164 he single-nucleotide change, create a unique restriction site in one of the alleles.

165 A single Eco47III restriction site in the 5' flanking region differentiate

166 One aphid clone bore a variant HindIII restriction site in the Buchnera leucine plasmid (pAPEle

167 e near the end of an element and an adjacent restriction site in the flanking genomic DNA.

168 This sequence alteration introduces a PleI restriction site in the nonsyphilis strains and thus all

169 rsonii reference strain 21038 but had a DraI restriction site in the spacer.

170 e substitution abolished the conserved HinfI restriction site in the two PCR fragments with unpredict

171 All but one mutation altered a restriction site in the URO-D gene, permitting identific

172 The specific protection of only one of many restriction sites in a genome from inactivation by a cog

173 Phylogenetic analysis of restriction sites in Culaea mtDNAs and haplotype-definin

174 e antibody-DNA combinations by incorporating restriction sites in DNA.

175 kelihood analysis of independent polymorphic restriction sites in humans reveals a decrease in effect

176 We took advantage of native restriction sites in SERT to construct a chimeric transp

177 y is based on the deprotection of methylated restriction sites in synthetic oligonucleotides and can

178 ocations of the EcoRI, SacII, EagI, and NotI restriction sites in the clones, resulting in a high-res

179 Ninety percent of the potential restriction sites in the constant regions and 47% of the

180 ore unaffected by the availability of useful restriction sites in the flanking region.

181 o the appearance of PstI and loss of HindIII restriction sites in the pol region as a result of sever

182 he constant regions and 47% of the potential restriction sites in the variable regions were present i

183 n the size and the lack of convenient unique restriction sites in these large-insert bacterial clones

184 codon optimization for any expression host, restriction site inclusion and exclusion, separation of

185 representing both minor gene editing events (restriction site insertion) to mimic gene correction, or

186 that facilitates the design of endonuclease restriction sites into the 5'-end of PCR primers.

187 orter system consisted of a series of unique restriction sites introduced into SV40 at nt 2666.

188 equence-targeted site in which the protected restriction site is embedded.

189 In this design, a restriction site is engineered in one of the two polymer

190 ifier: through additional silent mutations a restriction site is included or a previous one removed w

191 A PCR reaction is performed in which a restriction site is introduced in wild-type DNA by alter

192 The interconnecting restriction site junctions that are located at the ends

193 ic H1 gene (H1d) by introduction of suitable restriction sites just 5' of the TATA box and 3' of the

194 Methylation of NotI restriction sites located in HIC-1 was demonstrated in 2

195 Finally, analysis of phage L restriction site locations and a number of patches of nu

196 the gene order determined from whole genome restriction site mapping indicates that a high degree of

197 eumatoid arthritis patients and confirmed by restriction site mapping.

198 An approach to restriction-site mapping and contig building that uses f

199 uble-strand break and the donor chromosome's restriction site marker.

200 Restriction site markers in the chloroplast DNA reveal s

201 of locations at which RT switched templates, restriction site markers were introduced to divide the d

202 WH204 and WH221, containing eight different restriction site markers were used.

203 repeat was flanked by two different sets of restriction site markers.

204 odified HIV-1 (NL-Mme) containing a type IIS restriction site, MmeI, at the right end of viral DNA.

205 oligomer-based library with minimal need for restriction site modification of sequences in the target

206 methods that rely on uneven distributions of restriction site motifs in the DNA.

207 Of 117 restriction-site mutations shared among a subset of two

208 , the YAC clones revealed several additional restriction sites not previously detected in genomic DNA

209 single copy of Tn551 in the same chromosomal restriction site of both ISP479R and tPMPr transductants

210 d on theoretical grounds, Taq1 is the forced restriction site of choice.

211 ide duplex containing the iterated CG repeat restriction site of the NarI endonuclease has been deter

212 , we have developed protocols using the Notl restriction sites of any PAC or BAC clone to introduce a

213 Our analysis of DNA methylation-sensitive restriction sites of the 3' RR has revealed a similar mo

214 shifted in accordance with the locations of restriction sites on the construct.

215 iciency, nearly independent of the number of restriction sites on the plasmid.

216 drug resistance genes and of several unique restriction sites on the plasmids enabled rigorous genet

217 ental constructs or engineering of different restriction sites onto the ends of DNA fragments, are co

218 o either ineffective cleavage at the cognate restriction site or relaxed specificity allowing cleavag

219 n reaction primers, and does not require any restriction sites or use of restriction enzymes.

220 restriction enzymes, mutagenesis of internal restriction sites, or reamplification to add end homolog

221 limited by the presence of mutation-specific restriction sites, patients with neuropathy, ataxia and

222 Infrequent restriction site PCR (IRS-PCR) is a recently described D

223 d using genomic data generated by infrequent restriction site PCR and gene sequence analysis of the f

224 agment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid cont

225 plogroups was surveyed through the published restriction site polymorphism and control region sequenc

226 A PstI restriction site polymorphism in intron 2 between C57BL/

227 In addition, a restriction site polymorphism in the nuclear rRNA ITS-1

228 Length and restriction site polymorphism within a 2.8-kb threonine-

229 An AluI restriction site polymorphism within intron 4 was used t

230 were consistently distinguished by a Tsp45I restriction site polymorphism.

231 The frequencies of the restriction site polymorphisms at the human phenylalanin

232 sample of 31 Australian isolates by assaying restriction site polymorphisms from 11 protein encoding

233 detect an unprecedented number of genomewide restriction site polymorphisms from two N. crassa strain

234 A total of 10 restriction site polymorphisms have been identified at t

235 The use of silent restriction site polymorphisms indicates that the freque

236 e type of experiment, two phages marked with restriction site polymorphisms were crossed.

237 Seven restriction site polymorphisms were found in five of the

238 DNA (400 bp of hypervariable region 1 and 14 restriction site polymorphisms) and Y-chromosome (20 bia

239 f 301 polymorphic markers, consisting of 228 restriction site polymorphisms, 63 microsatellites, two

240 Using restriction site polymorphisms, and sequence-based nucle

241 A comparison with 30 restriction-site polymorphisms (RSPs) for which the ance

242 Variation is assessed by use of 30 autosomal restriction-site polymorphisms (RSPs), 60 autosomal shor

243 In the course of this study, restriction-site polymorphisms between 129S1 and CzechII

244 from classical polymorphisms as well as from restriction-site polymorphisms.

245 mages and data are presented that locate six restriction sites, predicted from gel electrophoresis, o

246 performs the following tasks in gene design: restriction site prediction, codon optimization for any

247 An in vitro Rsa I restriction site protection assay was developed that all

248 Gains of restriction sites relative to a known human mtDNA sequen

249 lly silent single-base mutations that create restriction sites (restriction fragment length polymorph

250 two homologous templates carrying different restriction sites separated by 282 bp.

251 the two alleles based on a polymorphic EarI restriction site showed that the Lys and Arg alleles had

252 r characterization of six ESRV loci revealed restriction sites specific for JSRV.

253 relatively inexpensive method called nested restriction site-specific PCR (RSS-PCR) that generates a

254 CR-mediated generation of an artificial AluI restriction site specifically with the CRM197 DNA templa

255 with newly incorporated expressed polylinker restriction sites still functions as a repressor.

256 We present the results of a restriction site survey of variation at five loci in Dro

257 This mutation produces a novel BstNI restriction site that can be used to identify individual

258 R3 exon 15, that would disrupt a unique BbsI restriction site that includes the Lys650 codon.

259 it, as the T8399G mutation creates a unique restriction site that is not present in wild-type human

260 A probe and target DNA anneal to create a restriction site that is recognized by a strand-specific

261 A region contains five groups of rare-cutter restriction sites that define CpG islands.

262 tial fraction of subtelomeric DNA containing restriction sites that is not digested with enzymes such

263 nce of a variable methylation pattern at the restriction sites that prevented proper enzyme cleavage

264 ively demonstrating the marker mutations and restriction sites that were engineered into the componen

265 r sequence motifs besides the canonical GATC restriction sites, thereby expanding the utility of this

266 (less than 3 kb); (ii) possess eleven unique restriction site to facilitate directional cloning; (iii

267 The length of duplex DNA from a unique restriction site to the single-stranded DNA region was t

268 of redundancy that allows the orders of most restriction sites to be determined, provides multiple da

269 utation sites sometimes present some natural restriction sites to differentiate the wild-type and mut

270 applying it to data from a recent survey of restriction site variation in the chloroplast genome of

271 No restriction site variation in the mitochondrial genome w

272 l mys subfamilies identified on the basis of restriction site variation occur in more than one specie

273 so enhanced integration events at random non-restriction sites via microhomology-mediated recombinati

274 nd methylation of a previously reported NotI restriction site was associated with dense CpG methylati

275 The average resolution of restriction sites was 48 kbp.

276 human motilin receptor containing 33 unique restriction sites was designed and stably coexpressed in

277 spectively, were analysed, and a total of 67 restriction sites was scored.

278 e-cutting restriction enzymes and engineered restriction sites was used to map the chromosome locatio

279 wo-amino acid linker (generated by fusion of restriction sites) was used to join VH and CH3.

280 polymorphism, which generates a unique BsrI restriction site, was detected at position 2073.

281 Using unique restrictions sites, we created additional chimeric molec

282 m the rates of recombination between the two restriction sites were 1 and 7% using Taq and Vent polym

283 P analysis also disclosed that the same AFLP restriction sites were digested in all of the fecal isol

284 the plasmids and because a number of unique restriction sites were present in the vector, studies we

285 , 8 affecting chromosome size and 3 altering restriction sites, were observed in these populations, w

286 s was constructed and five SNPs, residing in restriction sites, were selected for the development of

287 hylating short specific DNA sequences called restriction sites, whereas R recognizes unmethylated res

288 Such binding efficiently shields methylation/restriction sites which overlap with the bis-PNA binding

289 The loss of a Mnl1 restriction site, which results from the deletion, was u

290 lted from the occurrence in the VL cDNA of a restriction site, which was cleaved during construction

291 s of DNA sequence polymorphisms that disrupt restriction sites, which allows RAD tags to serve as gen

292 he triplet code to design genomes that avoid restriction sites while producing the same proteins as w

293 d have been able to measure the positions of restriction sites with a precision of approximately 1.5

294 gene were characterized at eight polymorphic restriction sites within the beta-globin gene cluster.

295 The lack of variation for the other restriction sites within the gene examined here indicate

296 This analysis showed that restriction sites within the gene were highly conserved,

297 Restriction sites within the region of homology allowed

298 The increased accessibility of restriction sites within these repetitive sequences is i

299 A survey of 24 restriction sites yielded 16 polymorphic sites and 21 di

300 pairs O6-methylguanine; PvuII cleaves at its restriction site, yielding a blunt-ended double strand,