ライフサイエンスコーパス: restrictive temperature

1 inability of myo2-E1 to form colonies at the restrictive temperature.

2 enesis and over multiple generations, at the restrictive temperature.

3 aughter cells immediately after the shift to restrictive temperature.

4 promotes rapid degradation of Cdc45p at the restrictive temperature.

5 d displays a pre-mRNA splicing defect at the restrictive temperature.

6 of genes are differentially expressed at the restrictive temperature.

7 ed mtDNA replication in the germarium at the restrictive temperature.

8 y1 allele replace those encoded by rsw1-1 at restrictive temperature.

9 l reduction in translation initiation at the restrictive temperature.

10 hydroxyurea or by the mutant arrests at the restrictive temperature.

11 ants that are blocked in this process at the restrictive temperature.

12 tants rebud and reduplicate their DNA at the restrictive temperature.

13 r photosynthetic ability at the 35 degrees C restrictive temperature.

14 ts are specifically lost from the complex at restrictive temperature.

15 p is diffusely localized in the cytoplasm at restrictive temperature.

16 following a shift from the permissive to the restrictive temperature.

17 n yeast TBP-associated factors (TAFs) at the restrictive temperature.

18 ain spindle integrity during anaphase at the restrictive temperature.

19 eins from the ER to the Golgi complex at the restrictive temperature.

20 bited in the srs2Delta sgs1-ts strain at the restrictive temperature.

21 on, is stabilized in the kar2 strains at the restrictive temperature.

22 TATA-less Pol II promoters is reduced at the restrictive temperature.

23 14(ts) cpt1 strains to grow at the sec14(ts) restrictive temperature.

24 a loss of growth polarity when incubated at restrictive temperature.

25 SA4 mRNAs that were newly synthesized at the restrictive temperature.

26 lation of HRP were markedly inhibited at the restrictive temperature.

27 ely 30% decrease in protein synthesis at the restrictive temperature.

28 and can complete the asexual growth cycle at restrictive temperature.

29 f invertase and the vacuolar protease CPY at restrictive temperature.

30 COP is degraded after cells are shifted to a restrictive temperature.

31 o permitted growth of plc1delta cells at the restrictive temperature.

32 MOB1 cause a late nuclear division arrest at restrictive temperature.

33 tely 50% in the CB286 ts mutant grown at the restrictive temperature.

34 ntromere chromatin structure is disrupted at restrictive temperature.

35 and SNAP-25, accumulates in comt mutants at restrictive temperature.

36 tly skipped by the splicing machinery at the restrictive temperature.

37 ion inhibits nuclear migration and growth at restrictive temperature.

38 produced after a shift of the mcb mutant to restrictive temperature.

39 ex (NPC) structure and nuclear import at the restrictive temperature.

40 gates, was not observed in ts85 cells at the restrictive temperature.

41 nt induction of a heat shock response at the restrictive temperature.

42 rsibly when vti1-1 cells were shifted to the restrictive temperature.

43 when mdm1 mutant cells are incubated at the restrictive temperature.

44 d receptors was mildly defective only at the restrictive temperature.

45 has no adverse effect on this process at the restrictive temperature.

46 yurea and display an S-phase arrest at their restrictive temperature.

47 lected by increased Poly(A)+ RNA export at a restrictive temperature.

48 verely inhibited in the pol3-3 mutant at the restrictive temperature.

49 nuclear export of Poly(A)+ RNA when grown at restrictive temperature.

50 retion phenotype when grown at the sec14(ts)-restrictive temperature.

51 oss of growth polarity when incubated at the restrictive temperature.

52 tion of noninfectious progeny virions at the restrictive temperature.

53 genesis following upshift from permissive to restrictive temperature.

54 ) Yersinia pseudotuberculosis strains at the restrictive temperature.

55 the SPB decrease in the mutant cells at the restrictive temperature.

56 and arrest in G2 after being shifted to the restrictive temperature.

57 chronized pol30 cells through S phase at the restrictive temperature.

58 mutants prepared from cells incubated at the restrictive temperature.

59 imD arrest with aberrant mitotic spindles at restrictive temperature.

60 apable of rescuing sec7 mutant growth at the restrictive temperature.

61 tant accumulates unspliced precursors at the restrictive temperature.

62 t1 cells show a pleiotropic phenotype at the restrictive temperature.

63 accumulate single-stranded DNA breaks at the restrictive temperature.

64 s59-Asn98del) completely failed to rescue at restrictive temperature.

65 are symmetric and markedly diminished at the restrictive temperature.

66 APC) arrest at metaphase of meiosis I at the restrictive temperature.

67 strains and increases their survival at the restrictive temperature.

68 plasmid can also rescue sid2-250 at the low restrictive temperature.

69 reflected by reduced viral replication at a restrictive temperature.

70 ng plasmid was then removed by growth at the restrictive temperature.

71 re sensitive mutant strain in RNase P at the restrictive temperature.

72 ype strain but not in a mutant strain at the restrictive temperature.

73 sed in the dU2AF50 mutant flies grown at the restrictive temperature.

74 display diminished levels of 60S subunits at restrictive temperature.

75 t in the Sec23p component of COPII) grown at restrictive temperature.

76 mbly-defective (fla) mutant fla11(ts) at the restrictive temperature.

77 rt all RNAs, including poly(A) mRNAs, at the restrictive temperature.

78 romosomes when expressed individually at its restrictive temperature.

79 r a temperature shift from the permissive to restrictive temperature.

80 h other components of the Brm complex at the restrictive temperature.

81 -phase of the cell cycle when shifted to the restrictive temperature.

82 temperature and subsequently analyzed at the restrictive temperature.

83 to complement the ftsQ1(Ts) mutation at the restrictive temperature.

84 ulated less efficiently after shifted to the restrictive temperature.

85 xhibit a block in ER-to-Golgi traffic at the restrictive temperature.

86 recruit the MCM complex to chromatin at the restrictive temperature.

87 rental efficiency at both the permissive and restrictive temperatures.

88 fail to accumulate in a subset of nuclei at restrictive temperatures.

89 henotype than did these cells cultured under restrictive temperatures.

90 ic ribosomal subunits at both permissive and restrictive temperatures.

91 s) allele enter the cell cycle and arrest at restrictive temperatures.

92 t permissive temperature, were functional at restrictive temperatures.

93 d from cells cultured at both permissive and restrictive temperatures.

94 thus synaptic transmission, at elevated, or restrictive temperatures.

95 the growth defect of the DerN321D mutant at restrictive temperatures.

96 ned operon allowed them to grow at otherwise restrictive temperatures.

97 ntion site in the mother cell after shift to restrictive temperatures.

98 mperatures below 20 degrees C, but at higher restrictive temperatures (26 to 29 degrees C) chromosoma

99 tional rsw1-1 root swelling phenotype at the restrictive temperature (29 degrees C).

100 erature, 19 degrees C, and pronounced at the restrictive temperature, 30 degrees C.

101 sin-II were found to not form zygotes at the restrictive temperature (32 degrees C).

102 ermissive temperatures (23 degrees C) to the restrictive temperature (37 degrees C), but hydroxyurea-

103 tron microscopy, 30 min after a shift to the restrictive temperature (37 degrees C), reveals a striki

104 Upon shift to restrictive temperature (37 degrees C), the PtdIns(4)P l

105 atures but becomes p60v-src-resistant at its restrictive temperature, 38 degrees C.

106 FT210 cells, which arrest at G2 phase at the restrictive temperature (39 degrees C), due to a mutatio

107 rmissive temperature (32.5 degrees C) to the restrictive temperature (39.5 degrees C), both small nuc

108 After transfer to a restrictive temperature (a heat shock), the level of the

109 l genome and subsequently transferred to the restrictive temperature, a DNA-packaging defect was evid

110 lta, and simultaneously became denser at the restrictive temperature, a hallmark of secretion-defecti

111 In rsw1-1 homozygotes at restrictive temperature, a striking dissociation of cell

112 ive growth and a G(2)/M cell cycle arrest at restrictive temperatures, a phenotype similar to that of

113 At restrictive temperatures (above 21 degrees) the mutation

114 In the mcb mutant growing at the restrictive temperature, actin patches are uniformly dis

115 with conditionally defective tropomyosin to restrictive temperatures, actin cables disappear within

116 erestingly, hsk1(ts) mutants released to the restrictive temperature after early S-phase arrest in hy

117 rupts its acetyl-transferase activity at the restrictive temperature, alters the transcription of spe

118 impaired in rRNA synthesis upon shift to the restrictive temperature, although the mechanism of inhib

119 ceptors was defective at both permissive and restrictive temperatures, although transactivation by gl

120 ormally but cannot complete cell division at restrictive temperature and arrest with decreased CTD ph

121 ele inhibited cortical actin motility at the restrictive temperature and eventually disrupted actin p

122 pld1 strains are not viable at the sec14(ts) restrictive temperature and exhibit a pattern of inverta

123 nsitivity (ts) limits viral replication at a restrictive temperature and may be involved with viral a

124 an sgs1 temperature-sensitive allele at the restrictive temperature and Slx1 and Slx4 proteins are s

125 ivide for three cell cycles after a shift to restrictive temperature and then arrest as a mixture of

126 mpletion of anaphase when cells are grown at restrictive temperature and this has been shown to be du

127 orp2-2 fail to complete DNA replication at a restrictive temperature and undergo cell cycle arrest.

128 feration of RPMI 8226 and HS Sultan cells at restrictive temperatures and growth arrest and increased

129 rstabilized in cdc34-3 and cdc4-3 mutants at restrictive temperatures and when S/T-P phosphorylation

130 tes first that the MSC is established at the restrictive temperature, and that melanoblasts die or lo

131 irst synchronized in M phase, shifted to the restrictive temperature, and then released from the bloc

132 not in the wild type after the shift to the restrictive temperature; and 3) the overexpression of Co

133 utation leads to delayed nuclear division at restrictive temperatures, apparently as a result of an i

134 epta formed in the mcb mutant growing at the restrictive temperature are mislocalized.

135 mitotic mutants, dim1-35 cells incubated at restrictive temperature arrest with low histone H1 kinas

136 splayed these phenotypes when shifted to the restrictive temperature at the appropriate developmental

137 n wild-type and tsaca2 cells, shifted to the restrictive temperature at various stages of development

138 ndergo cell cycle arrest when shifted to the restrictive temperature, becoming highly elongated.

139 ly in the second cycle after transfer to the restrictive temperature, blocking as large-budded cells

140 After shift to restrictive temperature, both mutants display impaired n

141 When shifted to the restrictive temperature, both mutants lose viability gra

142 At the restrictive temperature, both mutations resulted in aber

143 lements a conditional mutant of Cdc12 at the restrictive temperature, but arrests cells at the permis

144 t the mutation is due to loss of function at restrictive temperature, but molecular disruption of the

145 , many SIN mutants can be rescued at the low restrictive temperature by the osmotic stabilizer sorbit

146 chromosomal decatenase topoisomerase IV) at restrictive temperatures by high-copy suppressors is str

147 At the restrictive temperature, C309/UAS-shi(ts1) males formed

148 in a temperature-sensitive sec6 mutant at a restrictive temperature can be separated into at least t

149 coli, this ring assembly is impaired at the restrictive temperature causing lethal cell filamentatio

150 e-sensitive mcs6 allele was incubated at the restrictive temperature, Cdc2 was not activated and the

151 Following a shift to the restrictive temperature, cdc20 temperature-sensitive mut

152 At the restrictive temperature, cells defective in mitochondria

153 ive mtg3 mutants grown at the permissive and restrictive temperatures, combined with immunobloting wi

154 ic stability upon serial passage in vitro at restrictive temperatures compared to that of the parent

155 posure to UV radiation, cdc44 mutants at the restrictive temperature contain higher levels of single-

156 with anti-COPI antibodies, ldlF cells at the restrictive temperature could not be infected by vesicul

157 When shifted to restrictive temperature, dim1-35 mutant cells arrest bef

158 tains stable haploidy at both permissive and restrictive temperature, diploidizes at permissive tempe

159 At the restrictive temperature, double mutants carrying sen1-1

160 ve temperature, yet acts as a null allele at restrictive temperature due to loss of sir3-8 protein.

161 Shift of ceg1 mutants to restrictive temperature elicited a rapid decline in the

162 also blocked in the cell cycle such that at restrictive temperatures, esa1 mutants succeed in replic

163 t permissive temperature and then shifted to restrictive temperature exhibit severe reductions in fec

164 erience a delay in traversing S phase at the restrictive temperature following alpha factor arrest; a

165 tracts prepared after culturing cells at the restrictive temperature for 1 h indicates that the K151L

166 e ability to recover after being held at the restrictive temperature for approximately one day.

167 , TOP1 was not modified in ts85 cells at the restrictive temperature for its thermolabile ubiquitin-a

168 expressing the ts v-abl PTK and grown at the restrictive temperature for PTK activity the cells were

169 m-starved Rat-1 cells at both permissive and restrictive temperatures for p53.

170 ayed entry into S phase when released to the restrictive temperature from a G1 phase block.

171 similar mitotic defects when released to the restrictive temperature from an early S-phase block.

172 At the restrictive temperature, hsk1(ts) cells suffer abnormal

173 ve FliP protein continued to function at the restrictive temperature if incorporated at the permissiv

174 ate with Cdc28 when cells are blocked at the restrictive temperature in a cdc34 mutant, a point in th

175 complex is present at permissive but not at restrictive temperature in fla10 flagella.

176 e kinesin KHP1(FLA10), a protein inactive at restrictive temperature in fla10, a temperature-dependen

177 tant plasma membrane ATPase defective at the restrictive temperature in stability at the cell surface

178 At the restrictive temperature in the hsp82 mutant, the high af

179 At the restrictive temperature in the mutant, there is a decrea

180 basal RNA polymerase II transcription at the restrictive temperature in vitro.

181 a marked accumulation of docked vesicles at restrictive temperatures in comt.

182 occurred normally at both the permissive and restrictive temperatures in these mutants, the results s

183 iator cease transcription of all mRNA at the restrictive temperature, in a manner virtually indisting

184 unoblotting experiments indicate that at the restrictive temperature, inactivation of TAF25p function

185 of these MM cells under permissive, but not restrictive, temperatures increased the expression of p5

186 mutant still formed septa when grown at the restrictive temperature, indicating that polarized depos

187 and snR13T both increase in abundance at the restrictive temperature, indicating that Sen1p stabilize

188 ults in only partial rescue of growth at the restrictive temperature, indicating that splicing functi

189 ich leads to synthesis of increased dsRNA at restrictive temperature, induced apoptosis at restrictiv

190 Loss of separase function in rsw4 at the restrictive temperature is indicated by the widespread f

191 le of rescuing ts-38 (but not ts-114) at the restrictive temperature; it was demonstrated that homoge

192 At the restrictive temperature, Kog1 but not the Tor2 mutant pr

193 A shift to the restrictive temperature led to a cytoplasmic contraction

194 At a restrictive temperature, mipAD123 causes a slight, trans

195 of synchronized material revealed that at a restrictive temperature mipAD159 does not inhibit mitoti

196 le to that of a fimH insertion mutant at the restrictive temperature, mouse peritoneal macrophages we

197 At the restrictive temperature, mutant cells arrest in the cell

198 At restrictive temperature, mutant cells fail to express th

199 At the restrictive temperature, mutant cells progressively lose

200 At the restrictive temperature, mutant females are sterile.

201 cannot inhibit cellular protein synthesis at restrictive temperature no longer blocks Mnk1 binding to

202 tationary phase to exponential growth at the restrictive temperature of 30 degrees C and that this is

203 omyces cerevisiae that restore growth at the restrictive temperature of 30 degrees.

204 ut showed a complete loss of function at the restrictive temperature of 32 degrees C.

205 At the restrictive temperature of 36 degrees C, lid1-6 mutant c

206 e to divide after several generations at the restrictive temperature of 36 degrees C.

207 sensitive mRNA poly(A) polymerase grown at a restrictive temperature of 37 degrees C also contained a

208 eased from the blockade and incubated at the restrictive temperature of 37 degrees C, 95% of the cell

209 Upon shift of sec13-1 cells to the restrictive temperature of 37 degrees C, phospholipid sy

210 At the restrictive temperature of 37 degrees, ndc10-2 cells are

211 FH12/pFMH33 was able to grow at the restrictive temperature of 44 degrees C and FH12 lacking

212 s behavior below 15 degrees C-just above the restrictive temperature of mammalian fast axonal transpo

213 econd site mutations capable of reducing the restrictive temperature of the fission yeast mutant cdc2

214 is phenotype, disruption of HOC1 lowered the restrictive temperature of the pkc1-371 allele.

215 d, pre4-2 and ump1-2 strains fail to grow at restrictive temperatures on nonfermentable carbon source

216 of a temperature-sensitive ACA mutant at the restrictive temperature prevented c-di-GMP-induced cAMP

217 At the restrictive temperature, reg6 regenerating blood vessels

218 Returning the cells to the restrictive temperature restores the p53 protein levels,

219 of PtdIns(4)P observed in stt4(ts) cells at restrictive temperature result in a dramatic change in v

220 ts undergo chromosome mis-segregation at the restrictive temperature, resulting in a dramatic decreas

221 Incubation of the mcb mutant at restrictive temperature results in a three- to fivefold

222 Shifting sec7 strains to the restrictive temperature results in the disappearance of

223 ensitive allele gpa-16(it143), which, at the restrictive temperature, results in spindle orientation

224 In vivo observations of chromosomes at a restrictive temperature revealed that mipAD159 caused a

225 In contrast, repetitive stimulation at restrictive temperatures revealed a progressive, activit

226 r morphology in a trf4 (ts) trf5 mutant at a restrictive temperature reveals the presence of many cel

227 At the restrictive temperature, RNA replication was inhibited w

228 oping topless embryos between permissive and restrictive temperatures show that apical fates (cotyled

229 Plants containing this allele grown at the restrictive temperature showed weak radial swelling, wer

230 jections after exposure to pheromone; at the restrictive temperature, small budded cells accumulate.

231 elease experiments with cdc2.33 cells at the restrictive temperature, SPBs remained single, whereas i

232 re-sensitive alleles of SUG1 and SUG2 to the restrictive temperature strongly inhibited the expressio

233 be retained in hh(ts2) mutants raised at the restrictive temperature, suggesting it is not establishe

234 At restrictive temperature, temperature-sensitive nimA alle

235 nsitive nudG mutation grew no more slowly at restrictive temperature than a strain with only the CDHC

236 hydroxyurea sensitive and displayed a lower restrictive temperature than dpb11-1.

237 ects in mitotic and cell cycle regulation at restrictive temperatures that are apparently independent

238 At the restrictive temperature the add1 and add2 mutations disr

239 When synthesized at restrictive temperature the mutant chains formed an earl

240 At a restrictive temperature, the chromosomes of bimD6 mutant

241 At the restrictive temperature, the Cs- pol30 mutants undergo a

242 In a divK-cs mutant at the restrictive temperature, the initiation of DNA replicati

243 eases in sec14(ts) cki1 cells shifted to the restrictive temperature, the INO1 gene (encoding inosito

244 ts also accumulate unspliced pre-mRNA at the restrictive temperature, the mitotic arrest does not app

245 At a restrictive temperature, the mutant displayed reduced gr

246 Within 5 min of shifting to the restrictive temperature, the polarized distribution of s

247 At the restrictive temperature, the sec35-1 strain exhibits a t

248 At restrictive temperatures, the vacuoles of the mutant cel

249 hich neuroglian protein is mislocated at the restrictive temperature to an intracellular location in

250 we find that septin mutants incubated at the restrictive temperature trigger a Swe1-dependent mitotic

251 At the restrictive temperature, tsICP27 from LG4 fails to inhib

252 or zinc and recovered RNA replication at the restrictive temperature was isolated.

253 2 export was blocked in a gle1 mutant at the restrictive temperature, we propose a model wherein Gfd1

254 ing and septum in the sid2-250 mutant at low restrictive temperatures, we show that the lysis phenoty

255 nts were characterized in detail, and at the restrictive temperature were found to have an arrest phe

256 onic viability and larval development at the restrictive temperature were isolated.

257 eraction with the tsNIa-N393D protein at the restrictive temperature were recovered by a two-hybrid s

258 d extracts made from both the permissive and restrictive temperature were splicing competent.

259 at prevent ER-to-Golgi transport in vitro at restrictive temperatures were employed.

260 that prevent ER/Golgi transport in vitro at restrictive temperatures were used to selectively inacti

261 ons, as well as oncogene inactivation at the restrictive temperature when desired for functional stud

262 cold-sensitive mutant, Era(Cs) (E200K), at a restrictive temperature when expressed in a multicopy pl

263 he growth defects of some SIN mutants at low restrictive temperatures, where the SIN single mutants l

264 erature sensitive cesA1-1 allele (rsw1) at a restrictive temperature whereas mutations to A at these

265 mosomal copy of ctf13-30 fail to grow at the restrictive temperature, whereas a haploid strain carryi

266 oth nuclear division and polarity defects at restrictive temperature, which could be complemented by

267 a pronounced defect in rRNA biosynthesis at restrictive temperatures, while tRNA transcription and p

268 e cak1 mutant strains, which arrested at the restrictive temperature with nonuniform budding morpholo

269 We identified three short-sleep lines at the restrictive temperature with shared expression in the mu

270 leles of mps1 prevent SPB duplication at the restrictive temperature without affecting premeiotic DNA

271 t-off of protein synthesis upon shift to the restrictive temperature, without wholesale reduction in

272 At restrictive temperature, ypk1-1(ts) ykr2Delta cells lyse