ライフサイエンスコーパス: reticulocyte lysate

1 e potent inhibitors of translation in rabbit reticulocyte lysate.

2 heterocomplexes has been reconstituted from reticulocyte lysate.

3 length HRI synthesized de novo in the rabbit reticulocyte lysate.

4 he heme-regulated eIF2 alpha kinase (HRI) in reticulocyte lysate.

5 ient mRNA-protein fusion formation in rabbit reticulocyte lysate.

6 proteasome-dependent N-end rule pathway in a reticulocyte lysate.

7 atalytic protein subunit expressed in rabbit reticulocyte lysate.

8 refold luciferase in the presence of rabbit reticulocyte lysate.

9 ation of ornithine decarboxylase in a rabbit reticulocyte lysate.

10 ard ubiquitination and degradation in rabbit reticulocyte lysate.

11 mally denatured firefly luciferase in rabbit reticulocyte lysate.

12 ver, these proteins inhibited translation in reticulocyte lysate.

13 s in the GR.hsp90 heterocomplex assembled in reticulocyte lysate.

14 to bind eIF4E and to inhibit translation in reticulocyte lysate.

15 n-vitro transcription-coupled translation in reticulocyte lysate.

16 ut with the protein-folding system in rabbit reticulocyte lysate.

17 e to fold into globular proteins in a rabbit reticulocyte lysate.

18 embranes (95% rhodopsin) were incubated with reticulocyte lysate.

19 proteins by in vitro translation in a rabbit reticulocyte lysate.

20 to degradation in both lens fiber lysate and reticulocyte lysate.

21 xpression of the downstream reporter gene in reticulocyte lysate.

22 ssa in vitro translation system or in rabbit reticulocyte lysate.

23 he effect of novobiocin on Hsp90 function in reticulocyte lysate.

24 n translating target proteins with mammalian reticulocyte lysate.

25 aturation of luciferase and bind to Hsp70 in reticulocyte lysate.

26 e-regulated eIF2alpha kinase (HRI) in rabbit reticulocyte lysate.

27 tes that it binds to other factors in rabbit reticulocyte lysate.

28 rticoid receptors (GR) synthesized in rabbit reticulocyte lysates.

29 e translation assays carried out with rabbit reticulocyte lysates.

30 itiation step of protein synthesis in rabbit reticulocyte lysates.

31 ion of nsP4 produced in infected cells or in reticulocyte lysates.

32 ing that YY1 is readily polyubiquitinated in reticulocyte lysates.

33 proglobulins were synthesized from cDNAs in reticulocyte lysates.

34 degrees C than 30 degrees C, when tested in reticulocyte lysates.

35 utant (Q227L) activated Galphas expressed in reticulocyte lysates.

36 CAT inhibited translation in vitro in rabbit reticulocyte lysates.

37 the eta PKC isoenzyme synthesized in rabbit reticulocyte lysates.

38 east and with in vitro translation in rabbit reticulocyte lysates.

39 peptides produced by in vitro translation in reticulocyte lysates.

40 porter gene and assayed their translation in reticulocyte lysates.

41 hifting by 30-fold compared with 2.5-fold in reticulocyte lysates.

42 and eta PKC isoenzymes synthesized in rabbit reticulocyte lysates.

43 ctions are not known or cannot be assayed in reticulocyte lysates.

44 hepatoblastoma cells) or in vitro in rabbit reticulocyte lysates.

45 tallin to degradation in both lens fiber and reticulocyte lysates.

46 by an endogenous methyltransferase in rabbit reticulocyte lysates.

47 oteolytic degradation in both lens fiber and reticulocyte lysates.

48 lation of this mRNA both in cells and rabbit reticulocyte lysates.

49 promoted the degradation of rMGMT in rabbit reticulocyte lysates.

50 n by the purified kinase as well as by crude reticulocyte lysates.

51 r than the endogenous eIF4G concentration in reticulocyte lysates.

52 ranscription, translation and prenylation in reticulocyte lysates.

53 ed into an active complex in vitro in rabbit reticulocyte lysates.

54 sing chimeric reporter transcripts in rabbit reticulocyte lysates.

55 th an efficiency similar to that measured in reticulocyte lysates (40%), there were important qualita

56 e bound to alpha-crystallin was treated with reticulocyte lysate, a rich source of chaperones, up to

57 lation among TPMT half-life values in rabbit reticulocyte lysate, aggresome formation in COS-1 cells,

58 s degraded moderately in both lens fiber and reticulocyte lysates, alpha A(1-168)-crystallin was resi

59 tion of the in vitro oxidized mRNA in rabbit reticulocyte lysate also led to formation of SPs.

60 inding activity after incubation in a rabbit reticulocyte lysate also was substantially lower than th

61 Immunoadsorption of FKBP52 from reticulocyte lysate also yields co-immunoadsorption of c

62 of HspBP1 on renaturation of luciferase in a reticulocyte lysate and a defined system were examined.

63 which was post-translationally modified by a reticulocyte lysate and ATP-generating system.

64 h lacZ-CTA and ner-ACC were tested in rabbit reticulocyte lysate and E. coli to select UTRs that were

65 to greater than 80% homogeneity from rabbit reticulocyte lysate and has been given the name eIF4H.

66 and oxidized luciferase mRNA in both rabbit reticulocyte lysate and human HEK293 cells.

67 process, both in the complex environment of reticulocyte lysate and in a purified system.

68 is phosphorylated on Ser13 in situ in rabbit reticulocyte lysate and in cultured K562 cells and that

69 Mdm-2 in rabbit reticulocyte lysate and in normal, non-transformed 3T3 c

70 newly translated cyclin E, both in vitro in reticulocyte lysate and in vivo in human cells in cultur

71 onent of this assembly machinery in vitro in reticulocyte lysate and in vivo in Sf9 cells is p23.

72 of two reporter proteins, in vitro in rabbit reticulocyte lysate and in vivo in transfected BT7-H cel

73 e dimer is dependent upon factors present in reticulocyte lysate and other cytosols.

74 [3H]FK506, we found that both rabbit PP5 in reticulocyte lysate and purified rat PP5 were specifical

75 e RNA specificity of onconase in vitro using reticulocyte lysate and purified RNA substrates indicate

76 ation/translocation experiments using rabbit reticulocyte lysate and rough microsomes revealed that t

77 GCF2 expressed in vitro using reticulocyte lysates and Escherichia coli migrates as a

78 n vitro assembly systems derived from rabbit reticulocyte lysates and human cell extracts.

79 h the RNA subunit hTR in two systems (rabbit reticulocyte lysates and human cell lines) with respect

80 d a procedure for purifying eIF6 from rabbit reticulocyte lysates and immunochemically characterized

81 d/or assembly of proglobulin trimers both in reticulocyte lysates and in seeds.

82 dthrough in vitro in wheat germ extracts and reticulocyte lysates and in vivo in oat protoplasts.

83 bited Mos-mediated MAPK activation in rabbit reticulocyte lysates and repressed MKK activation by v-M

84 sufficient to inhibit translation in rabbit reticulocyte lysates and sufficient to inhibit reporter

85 e resulting mutants were expressed in rabbit reticulocyte lysates and their interaction with HBcAg wa

86 es, occurred with equal efficiency in rabbit reticulocyte lysates and was much enhanced over that exh

87 0-2 AGG interruptions, both in vitro (rabbit reticulocyte lysates) and in cell culture (HEK-293 cells

88 exes at an intermediate stage of assembly in reticulocyte lysate, and Hip is also thought to be an in

89 -ACC) enhanced protein synthesis in a rabbit reticulocyte lysate, and it was compared to a lacZ-CTA,

90 nsisting of in vitro-transcribed RNA, rabbit reticulocyte lysate, and microsomal membranes on the bas

91 igo(A) tail are translated equally well in a reticulocyte lysate, and their translation is stimulated

92 DAPK suppresses translation in rabbit reticulocyte lysate, and treatment of neuroblastoma cell

93 by incubating immunopurified p53 with rabbit reticulocyte lysate, and we show by peptide competition

94 bition of eRF1 enhanced PRF in yeast, rabbit reticulocyte lysates, and mammalian cells.

95 eta-Tubulin mutants were expressed in rabbit reticulocyte lysates, and the effect of C-terminal, N-te

96 ncated s4 mRNAs were translated using rabbit reticulocyte lysates, and translation product sigma3 was

97 ly, in a degradation system utilizing rabbit reticulocyte lysate, antizyme 1 (AZ1) accelerates protea

98 nsistent with the effects observed in rabbit reticulocyte lysate, application of geldanamycin to fibr

99 st that these two conjugated proteins of the reticulocyte lysate are specific substrates for isopepti

100 haracteristics to eIF4H purified from rabbit reticulocyte lysate as described previously.

101 determined using both lens fiber lysate and reticulocyte lysate as sources of ubiquitinating and pro

102 determined using both lens fiber lysate and reticulocyte lysate as sources of ubiquitinating and pro

103 order Q70E/Q162E>Q162E> Q70E=WT betaB2 using reticulocyte lysate as the source of degradation machine

104 of translational repression, we used rabbit reticulocyte lysates as an in vitro translation system t

105 translation of the target mRNA in the rabbit reticulocyte lysate assay, but not in the cell-based ass

106 of protein synthesis in the cell-free rabbit reticulocyte lysate assay, degrading tRNA at concentrati

107 BAG-1 was present in reticulocyte lysate at a BAG-1:hsp70/hsc70 molar ratio o

108 70, RPS3, and NF45) were expressed in rabbit reticulocyte lysate, bacteria, and MCF-7 cells.

109 70, we purified a 38-kDa protein from rabbit reticulocyte lysate based upon its ability to stimulate

110 The DGD protein expressed in rabbit reticulocyte lysates bound truncated DHBV pre-S protein

111 Pr55(gag) was synthesized in reticulocyte lysates, bound to membranes, and incubated

112 t SBP2 is the only limiting factor in rabbit reticulocyte lysate but not in transfected rat hepatoma

113 04 cooperates with the chaperones present in reticulocyte lysates but not with DnaK of E. coli.

114 ptor-hsp90 heterocomplex is brought about in reticulocyte lysate by a preformed protein-folding compl

115 firefly luciferase readily occurs in rabbit reticulocyte lysate by an ATP-dependent process.

116 The addition of recombinant PEK to reticulocyte lysates caused a dose-dependent inhibition

117 n in each variant was quantified in a rabbit reticulocyte lysate cell-free translation system supplem

118 RNA and to inhibit translation in the rabbit reticulocyte lysate compared to its counterpart containi

119 cription/translation system utilizing rabbit reticulocyte lysates confirmed the interaction of NQO1 a

120 itro experiments using proteins generated in reticulocyte lysates confirmed this interaction and indi

121 d that GR.hsp90 heterocomplexes assembled in reticulocyte lysate contain cytoplasmic dynein in a mann

122 tory complex subunits, S2, was translated in reticulocyte lysate containing [(35)S]methionine and use

123 of these putative ATPases was synthesized in reticulocyte lysate containing [35S]methionine, and the

124 length p56(lck) molecules produced in rabbit reticulocyte lysate containing active chaperone machiner

125 e expressed these chimeras by translation in reticulocyte lysate containing canine pancreatic microso

126 biquitination relative to its translation in reticulocyte lysates containing 125I-ubiquitin.

127 ncreased when translation was carried out in reticulocyte lysates containing high K+ concentrations,

128 vitro degradation of a target mRNA in rabbit reticulocyte lysates containing in vitro-translated Vhs.

129 Reticulocyte lysate contains a chaperone system that ass

130 Rabbit reticulocyte lysate contains a multiprotein chaperone sy

131 Rabbit reticulocyte lysate contains a multiprotein chaperone sy

132 Rabbit reticulocyte lysate contains a multiprotein system that

133 0, is imported by isolated mitochondria in a reticulocyte lysate-dependent manner.

134 B59 protein synthesized in vitro in a rabbit reticulocyte lysate dephosphorylated rat ERK1 and ERK2 p

135 ized seven members of the family in a rabbit reticulocyte lysate, determined their Stokes radius, sed

136 ranslation of these fusion vectors in rabbit reticulocyte lysate +/- dog pancreatic microsomes follow

137 tive of whether it was synthesized in rabbit reticulocyte lysate, Escherichia coli or Trichoplusia ni

138 parately synthesized in vitro using a rabbit reticulocyte lysate expression system.

139 In assays using Hop-depleted rabbit reticulocyte lysate for the cell-free assembly of recept

140 , by the endogenous ubiquitinating system in reticulocyte lysate fraction II, and by intact HEK293 ce

141 ked to an unidentified protein in the rabbit reticulocyte lysate, generating a product slightly large

142 In the presence of rabbit reticulocyte lysate, grp170 can refold and partially res

143 We show that rabbit reticulocyte lysate harbors enzymatic components that ca

144 tein synthesizing system that employs rabbit reticulocyte lysates has been employed for protein produ

145 in that wheat hsp70 functions in the rabbit reticulocyte lysate heterocomplex assembly system and hu

146 ed in an expression system coupled to rabbit reticulocyte lysate in the presence or absence of dog pa

147 ous v-Mos mutants were expressed in a rabbit reticulocyte lysate in vitro translation system and in C

148 rboxyl-terminal domain of ICP0 to the rabbit reticulocyte lysate in vitro translation system resulted

149 ties of RSV mutants translated in the rabbit reticulocyte lysate in vitro translation system.

150 accumulation of heavy polymeric ribosomes in reticulocyte lysates in vitro.

151 tin in a cell-free expression system (rabbit reticulocyte lysate) increased by 40%.

152 d-type Pak2 in cells and addition of Pak2 to reticulocyte lysate inhibit translation, while kinase-in

153 his work, we separate the proteins of rabbit reticulocyte lysate into three fractions by DEAE chromat

154 epressed ferritin synthesis 4-fold in rabbit reticulocyte lysates (IRP1 + IRP2), confirming differenc

155 ge activity of NS2/3 protease synthesized in reticulocyte lysate is ATP-dependent, as evidenced by AT

156 thase by the native ubiquitinating system of reticulocyte lysate is dependent upon both Hsp70 and the

157 impression by showing that all of the Hop in reticulocyte lysate is present in an hsp90.Hop.hsp70 com

158 When a rabbit reticulocyte lysate is programmed with uncapped lucifera

159 The apo-nNOS activating activity of reticulocyte lysate is retained in a pool of fractions c

160 Furthermore, translation activity in rabbit reticulocyte lysate is strongly inhibited by RNAs exceed

161 in, G beta, synthesized in vitro in a rabbit reticulocyte lysate, is unable to fold into a native str

162 After its in vitro synthesis in rabbit reticulocyte lysate, it can also be efficiently imported

163 translation of intact sIL-1Ra cDNA in rabbit reticulocyte lysates led to both pro-sIL-1Ra and icIL-1R

164 Use of reticulocyte lysates manipulated to deplete them of eIF4

165 M-AS inhibited translation in vitro (rabbit reticulocyte lysate) of target mRNA at concentrations as

166 e have isolated a protein kinase from rabbit reticulocyte lysates on the basis of its ability to phos

167 We isolated a protein kinase from rabbit reticulocyte lysates on the basis of its ability to phos

168 ependent translation when used to supplement reticulocyte lysate; one of these activities was identif

169 ere we show that immunodepletion of Hip from reticulocyte lysate or addition of high levels of Hip to

170 ) complex of chaperone proteins is formed in reticulocyte lysate or from purified proteins.

171 n then be refolded by the addition of rabbit reticulocyte lysate or hsc70 and Hdj-1, whereas Hdj-1 do

172 nthesized substrate proteins bound to CCT in reticulocyte lysate or intact Xenopus oocytes.

173 novo in cell-free systems containing rabbit reticulocyte lysate or wheat germ extracts assembles int

174 nterestingly, we find that pre-incubation of reticulocyte lysates or ribosomal salt wash fractions wi

175 we found that an activity present in rabbit reticulocyte lysates phosphorylates and activates Chk2.

176 fied VHL-CCT complexes, when added to rabbit reticulocyte lysate, proceeded to form VCB and VCB-Cul2.

177 Rabbit reticulocyte lysates programmed with monocistronic RNAs

178 in the translation mixture by the endogenous reticulocyte lysate proteasome.

179 h a source of ubiquitination enzymes (rabbit reticulocyte lysate), purified ubiquitin, and ATP reveal

180 e detected by electron microscopy within the reticulocyte lysate reaction mixtures and appeared essen

181 An in vitro assay with reticulocyte lysate recapitulated the release of intact

182 Expressed in rabbit reticulocyte lysate, recombinant p133 and telomerase RNA

183 Using an eIF4G-depleted reticulocyte lysate, reconstitution with mock-phosphoryl

184 in vitro translation of the mRNAs in rabbit reticulocyte lysate reflected differences in the positio

185 erminal variants of RGS4 that were stable in reticulocyte lysate remained unstable in L cells.

186 ression of the ORF 3 coding region in rabbit reticulocyte lysates resulted in the production of a sin

187 In vitro translation of rabbit reticulocyte lysate ribosomes was inhibited by the C-ter

188 incubated with both exogenous ATP and rabbit reticulocyte lysate (RRL) as a source of ubiquitin-prote

189 We used a rabbit reticulocyte lysate (RRL) cell-free system to examine tr

190 iation factor 2 (HRI) is activated in rabbit reticulocyte lysate (RRL) in response to a number of env

191 ha kinase (HRI) in hemin-supplemented rabbit reticulocyte lysate (RRL) in response to heat and oxidat

192 la, was translated in vitro in either rabbit reticulocyte lysate (RRL) or wheat germ extract (WGE).

193 mally denatured firefly luciferase in rabbit reticulocyte lysate (RRL) requires hsp90, hsc70, and oth

194 d prey proteins (probes) in cell-free rabbit reticulocyte lysate (RRL) systems.

195 In a rabbit reticulocyte lysate (RRL) translation system, a mutant A

196 inhibit Hsc70-dependent processes in rabbit reticulocyte lysate (RRL) was examined.

197 expressed in a mammalian cell lysate, rabbit reticulocyte lysate (RRL), was able to assemble into cap

198 ver, it did not affect translation in rabbit reticulocyte lysate (RRL).

199 (HRI) interacts with hsp90 in situ in rabbit reticulocyte lysate (RRL).

200 ent in mock and Ric-8A-immunodepleted rabbit reticulocyte lysate (RRL).

201 activated not only in heme-deficient rabbit reticulocyte lysates (RRL), but also in hemin-supplement

202 f the 750-kD keratin complex, we used rabbit reticulocyte lysates (RRL).

203 cription/translation system employing rabbit reticulocyte lysates (RRLs).

204 Translation of RNAs in reticulocyte lysates showed that cleavage at the nsP3/ns

205 The binding of AIP to AhR in reticulocyte lysate shows several of the characteristics

206 1 and STAT2 or STAT1 and STAT3 translated in reticulocyte lysate spontaneously form heterocomplexes w

207 lation of in vitro translated Rab5 in rabbit reticulocyte lysate, suggesting that it competes for lip

208 fused to glutathione S-transferase in rabbit reticulocyte lysates, suggesting a role for the pU(L)34/

209 ram a cell-free translation system of rabbit reticulocyte lysates supplemented with canine pancreas m

210 ation by using purified Pr55(Gag) and rabbit reticulocyte lysate-synthesized Tsg101, and (iii) in viv

211 nslation products of these fusion vectors in reticulocyte lysate system +/- microsomal membranes were

212 n was performed using [(35)S]methionine in a reticulocyte lysate system +/- microsomes, and the trans

213 hBVR translated by the rabbit reticulocyte lysate system appears on a nondenaturing ge

214 ts from control and diabetic adipocytes to a reticulocyte lysate system demonstrated the inhibition o

215 antigen can rescue protein synthesis in the reticulocyte lysate system from inhibition by low concen

216 ag proteins synthesized in vitro in a rabbit reticulocyte lysate system in the absence of exogenous l

217 pitation of proteins in vitro expressed in a reticulocyte lysate system showed an interaction between

218 In the reticulocyte lysate system the W RNAs shut off protein s

219 Using a cell-free reticulocyte lysate system we have examined the relation

220 ng translation in vitro in a standard rabbit reticulocyte lysate system, although all of the other vi

221 c proteins with a substrate synthesized in a reticulocyte lysate system, before its posttranslational

222 Using a rabbit reticulocyte lysate system, E2EPF was shown to support t

223 Thus, in the rabbit reticulocyte lysate system, the c-jun 5' UTR likely impe

224 imeric and truncated proteins expressed in a reticulocyte lysate system, we have identified two topog

225 Through the use of an eIF4G-depleted reticulocyte lysate system, we show that this presumptio

226 opomyosin 3'UTR RNA was observed in a rabbit reticulocyte lysate system, which is known to contain en

227 with masses of 26 and 17 kDa) formed in the reticulocyte lysate system.

228 ells and Rep78 produced in vitro in a rabbit reticulocyte lysate system.

229 a and in a coupled transcription/translation reticulocyte lysate system.

230 anslation was assessed in an in vitro rabbit reticulocyte lysate system.

231 nhibition of protein synthesis in the rabbit reticulocyte lysate system.

232 The proteins were expressed in vitro in a reticulocyte lysate system.

233 pe and mutant proteins incubated in a rabbit reticulocyte lysate system.

234 on in both the wheat germ extract and rabbit reticulocyte lysate systems.

235 minimal chaperone system reconstituted from reticulocyte lysate that forms glucocorticoid receptor (

236 When added to rabbit reticulocyte lysate that has been depleted of endogenous

237 inant telomerase requires a factor in rabbit reticulocyte lysate that promotes ribonucleoprotein asse

238 iated with a kinase endogenous to the rabbit reticulocyte lysate that was identified as PAK-2, (ii) N

239 are Gbeta isoform-specific factors in rabbit reticulocyte lysates that determine the efficacy of Gbet

240 pitulated in an in vitro system using rabbit reticulocyte lysates that had been largely depleted of e

241 Protein complexes in reticulocyte lysates that specifically interact with the

242 We find, using rabbit reticulocyte lysates, that the presence of the c-jun 5'

243 rative support of p56lck structure in rabbit reticulocyte lysate, the specific occurrence of complexe

244 , we utilized pulse-chase analyses in rabbit reticulocyte lysate to demonstrate that hsp90-bound inte

245 nly in the templating region and used rabbit reticulocyte lysates to reconstitute telomerase activity

246 In a rabbit reticulocyte lysate transcription/translation system, th

247 nts co-migrated with fragments formed in the reticulocyte lysate translation mixture used for GAP-43

248 In this study, a rabbit reticulocyte lysate translation reaction was used to rec

249 ing frame is highly efficient in both rabbit reticulocyte lysate translation reactions and in culture

250 lenocysteines can be reconstituted in rabbit reticulocyte lysate translation reactions.

251 Rs can be synthesized in vitro with a rabbit reticulocyte lysate translation system supplemented with

252 oring translation to a PABP-dependent rabbit reticulocyte lysate translation system.

253 and inhibit globin translation in the rabbit reticulocyte lysate translation system.

254 nto an immature capsid when synthesized in a reticulocyte lysate translation system.

255 Western blot analysis of rabbit reticulocyte lysate using polyclonal antibody against th

256 protein synthesis, was purified from rabbit reticulocyte lysates using an assay that specifically me

257 mobility of GR recovered on incubation with reticulocyte lysate was inhibited by geldanamycin, a dru

258 The inhibition of translation observed in reticulocyte lysates was prevented by the addition of ad

259 ere was efficient reinitiation in a standard reticulocyte lysate, when initiation would be largely dr

260 2A10, recognises a protein present in rabbit reticulocyte lysate which binds murine p53 translated in

261 ent homogeneity a 66-kDa protein from rabbit reticulocyte lysate which is associated with hsp 70.

262 ated by the DE52-retained fraction of rabbit reticulocyte lysate, which also assembles nNOS.hsp90 het

263 ate investigation of these questions, rabbit reticulocyte lysate, which contains the cytoplasmic chap

264 tion substrates in fraction II of the rabbit reticulocyte lysate with an efficiency parallel to their

265 When PABP was depleted from the reticulocyte lysate with anti-human PABP antibody, the c

266 90.p60.hsp70 foldosome complex isolated from reticulocyte lysate with antibody against p60.

267 Mutant NQO1 incubated in rabbit reticulocyte lysate with MG132 resulted in the accumulat

268 Supplementation of rabbit reticulocyte lysate with nucleolin decreased APP mRNA st

269 radation of ubiquitinated proteins in rabbit reticulocyte lysates with an IC50 of 52 microM.

270 al inhibition of brain poly(A) RNA in rabbit reticulocyte lysate without accelerated mRNA degradation

271 ted their translation efficiencies in rabbit reticulocyte lysates, Xenopus oocytes, and primary cultu