ライフサイエンスコーパス: retinol dehydrogenase

1 of dehydrogenases that can oxidize retinol (retinol dehydrogenases).

2 specificities of the photoreceptor all-trans-retinol dehydrogenase.

3 cycle and as a substrate carrier for 11-cis-retinol dehydrogenase.

4 11-cis-retinyl-ester synthase, and an 11-cis-retinol dehydrogenase.

5 nzymes, lecithin retinol acyltransferase and retinol dehydrogenases.

6 th human enzymes as physiologically relevant retinol dehydrogenases.

7 family and shares 65% sequence identity with retinol dehydrogenase 1 (RoDH1), which catalyzes the oxi

8 hort-chain dehydrogenase/reductase isozymes (retinol dehydrogenases 1-3 and CRAD1), closely related i

9 In embryos overexpressing aldh1a2 or retinol dehydrogenase 10 (rdh10) in the presence of thei

10 ed that oxidation of vitamin A to retinal by retinol dehydrogenase 10 (RDH10) is critical for embryon

11 nstrates that DHRS3 requires the presence of retinol dehydrogenase 10 (RDH10) to display its full cat

12 Human retinol dehydrogenase 10 (RDH10) was implicated in the o

13 Here, we sought to determine whether retinol dehydrogenase 10 (RDH10), upregulated in rod/con

14 of at least two subunits of NAD(+)-dependent retinol dehydrogenase 10 (RDH10), which catalyzes the ox

15 y, we discovered that mice with mutations in retinol dehydrogenase 10 (Rdh10), which perturbs Vitamin

16 B1 (Cyp26b1), which results in excess RA, or retinol dehydrogenase 10 (Rdh10), which results in RA de

17 study demonstrate that ectopic expression of retinol dehydrogenase 10 (RDH10, SDR16C4) in skin rafts

18 , c.C199T:p.R67* and c.C322T:p.R108*, in the retinol dehydrogenase 11 (RDH11) gene, resulting in a no

19 genase/reductase 1, retinal reductase 1, and retinol dehydrogenase 11.

20 retinoid visual cycle is catalyzed by 11-cis-retinol dehydrogenases (11-cis-RDHs) that oxidize 11-cis

21 This reaction is catalyzed by 11-cis-retinol dehydrogenases (11-cis-RDHs), prior to the chrom

22 Retinol dehydrogenase 12 (RDH12) is a novel member of th

23 Retinol dehydrogenase 12 (RDH12) is an NADP(+)-dependent

24 Retinal levels of retinol dehydrogenase 12 (RDH12) were measured by immuno

25 heca cells were aldehyde dehydrogenase 6 and retinol dehydrogenase 2, which play a role in all-trans-

26 NA library encodes a 317-amino acid protein, retinol dehydrogenase 4 (RoDH-4), which exhibits the str

27 retinol/sterol substrate specificity, namely retinol dehydrogenase 4 (RoDH4, SDR9C8), RoDH-like 3alph

28 pression of two retinoid biosynthesis genes: retinol dehydrogenase 5 (RDH5) and retinol dehydrogenase

29 Retinol dehydrogenase 5 (RDH5) is responsible for a majo

30 PE65 operates in a multiprotein complex with retinol dehydrogenase 5 and retinal G protein-coupled re

31 Levels of retinol dehydrogenase 5, and dehydrogenase/reductase sho

32 ream, attributable to mass action by CRALBP, retinol dehydrogenase 5, and high affinity of opsin apop

33 P-binding cassette transporter 4 (Abca4) and retinol dehydrogenase 8 (Rdh8) activities.

34 e roles of two chromophore-binding proteins, retinol dehydrogenase 8 (RDH8) and photoreceptor-specifi

35 eport that two chromophore binding proteins, retinol dehydrogenase 8 (RDH8) and photoreceptor-specifi

36 P-binding cassette transporter 4 (ABCA4) and retinol dehydrogenase 8 (RDH8), proteins critical for al

37 ng cassette transporter 4 (Abca4) and enzyme retinol dehydrogenase 8 (Rdh8), proteins critical for al

38 TP-binding cassette transporter 4) and RDH8 (retinol dehydrogenase 8), manifested retinal abnormaliti

39 retinoic acid synthesis, whereas microsomal retinol dehydrogenase (a short-chain dehydrogenase/reduc

40 brane-associated retinaldehyde reductase and retinol dehydrogenase activities are decreased by approx

41 All-trans retinol dehydrogenase activity assays were performed usi

42 The NADPH-dependent all-trans-retinol dehydrogenase activity in isolated RPE microsoma

43 horylated and reduced forms of NAD-dependent retinol dehydrogenase activity may function in the nucle

44 In turn, the retinol dehydrogenase activity of RDH10 is reciprocally

45 In rMC-1 cells, all-trans retinol dehydrogenase activity was detected in the micro

46 In addition to retinol dehydrogenase activity, RDH1 has strong 3alpha-h

47 g the control of RA production by APC is via retinol dehydrogenase activity.

48 de the first genetic evidence of a candidate retinol dehydrogenase affecting either vitamin A-related

49 involves two sequential steps, catalyzed by retinol dehydrogenases and retinal dehydrogenases, respe

50 Therefore, multiple retinol dehydrogenases are involved in regeneration of 1

51 s generated in the RPE, because no all-trans retinol dehydrogenase (atRDH) has been identified in the

52 tion of a putative RDH, referred to as RDHB (retinol dehydrogenase B), which functions in the visual

53 DH) has been shown to function in vitro as a retinol dehydrogenase catalyzing the synthesis of retino

54 We identified Rdh5 as a retina-specific retinol dehydrogenase controlled by APC.

55 Recently, we identified a cis-retinol dehydrogenase (cRDH) that oxidizes 9-cis-retinol

56 ied by mass spectrometric analysis as 11-cis-retinol dehydrogenase (cRDH), and enzymatic assays have

57 of cellular retinol-binding protein (Crbp), retinol dehydrogenase (Dhrs9/eRoldh), retinal dehydrogen

58 l-trans-retinol dehydrogenase (photoreceptor retinol dehydrogenase) displays identical stereospecific

59 molecularly cloned; here we focus on 11-cis retinol dehydrogenase (encoded by the gene RDH5; chromos

60 that requires the action of a zinc-dependent retinol dehydrogenase enzyme.

61 nto its active form, retinoic acid, requires retinol dehydrogenase enzymes.

62 g evidence that Xenopus rdhe2 functions as a retinol dehydrogenase essential for frog embryonic devel

63 A novel all-trans-retinol dehydrogenase exists in the RPE and performs a c

64 ent-binding proteins and showed that it is a retinol dehydrogenase expressed in non-ocular tissues su

65 RDH11 and RDH12 are closely related retinol dehydrogenases expressed in the retina.

66 rate retinal generated in situ by microsomal retinol dehydrogenases, from the physiologically most ab

67 Recombinant mutant 11-cis retinol dehydrogenases had reduced activity compared wit

68 strate in the absence of apo-CRBP microsomal retinol dehydrogenases have the higher specific activity

69 of apo-CRBP, a potent inhibitor of cytosolic retinol dehydrogenases (IC50 = approximately 1 microM),

70 Here we describe a third retinol dehydrogenase in the RPE, RDH10, which can produ

71 o function as a substrate carrier for 11-cis-retinol dehydrogenase in the synthesis of 11-cis-retinal

72 generation of rhodopsin by inhibiting 11-cis-retinol dehydrogenase in the visual cycle.

73 plished by a family of enzymes termed 11-cis-retinol dehydrogenases, including RDH5 and RDH11.

74 his result is unusual, because photoreceptor retinol dehydrogenase is a member of a short chain alcoh

75 ely catalyzed by abundantly expressed 11-cis-retinol dehydrogenase, is pro-S-specific to both 11-cis-

76 hares close amino acid similarity with mouse retinol dehydrogenase isozyme types 1 and 2 and CRAD1 (8

77 res closest amino acid similarity with mouse retinol dehydrogenase isozymes types 1 and 2 (86 and 91%

78 Unlike recombinant retinol dehydrogenase isozymes, recombinant CRAD was inh

79 ntensely in kidney and liver, in contrast to retinol dehydrogenase isozymes, which show strong mRNA e

80 uced neuritogenesis was partly attenuated in retinol dehydrogenase knockout (Rdh12(-/-)) mice and by

81 is genes: retinol dehydrogenase 5 (RDH5) and retinol dehydrogenase L (RDHL) in colon adenomas and car

82 result of the transcriptional repression of retinol dehydrogenase l1 via a complex that includes Lef

83 ll-trans-retinol via reversible oxidation by retinol dehydrogenases, members of the short-chain dehyd

84 which is the product of action by the enzyme retinol dehydrogenase on all-trans retinal, was not toxi

85 for chromophore regeneration and requires a retinol dehydrogenase, PDH, in retinal pigment cells.

86 A recently identified all-trans-retinol dehydrogenase (photoreceptor retinol dehydrogena

87 mal retinol dehydrogenases, versus cytosolic retinol dehydrogenases, provide the quantitatively major

88 Photoreceptor-specific retinol dehydrogenase (prRDH) catalyzes reduction of all

89 Photoreceptor retinol dehydrogenase (prRDH) is a membrane-associated c

90 ietary supplementation of retinaldehyde, and retinol dehydrogenase (RDH) activity assays, we demonstr

91 -induced internalization of rhodopsin, and a retinol dehydrogenase (RDH) that catalyzes the first ste

92 Retinol dehydrogenase (RDH), the enzyme that catalyzes t

93 ated with Stargardt macular degeneration and retinol dehydrogenases (RDH) in the clearance of all-tra

94 RDH12 has been suggested to be one of the retinol dehydrogenases (RDH) involved in the vitamin A r

95 t neurons, biosynthesize atRA using multiple retinol dehydrogenases (Rdh) of the short chain dehydrog

96 esis at the rate-limiting step, catalyzed by retinol dehydrogenases (RDH).

97 erns in adult tissues and embryos of a mouse retinol dehydrogenase, RDH1.

98 ism, which is catalyzed in large part by the retinol dehydrogenase RDH10, is critical for the spatiot

99 The microsomal retinol dehydrogenase (RDH11) and cytosolic soluble alde

100 three enzymes from a novel subfamily of four retinol dehydrogenases (RDH11-14) that display dual-subs

101 Herein, we report that the novel retinol dehydrogenase, rdh1l, is required for proper gut

102 the gut by regulating the expression of the retinol dehydrogenase, rdh1l.

103 henotype associated with mutations in 11-cis-retinol dehydrogenase (RDH5) causing fundus albipunctatu

104 etinol and as a substrate carrier for 11-cis-retinol dehydrogenase (RDH5).

105 release, all-trans-retinal is reduced by the retinol dehydrogenase RDH8 to all-trans-retinol in an NA

106 amily, frog sdr16c5, acts as a highly active retinol dehydrogenase (rdhe2) that promotes retinoic aci

107 hat human colon adenomas and carcinomas lack retinol dehydrogenases (RDHs) and that APC regulates the

108 biosynthesis of retinoic acid from retinal, retinol dehydrogenases (RDHs), access retinol bound to c

109 ns isolated from Rbp1(-/-) mice have altered retinol dehydrogenase/reductase (Rdh) enzyme activity th

110 en shown that mutations in RDH12, encoding a retinol dehydrogenase, result in severe and early-onset

111 the strongest similarity with rat all-trans-retinol dehydrogenases RoDH-1, RoDH-2, and RoDH-3, and m

112 Previously, two genes encoding retinol dehydrogenases (RoDH), which recognize holo-cell

113 properties; yet ethanol inhibited cytosolic retinol dehydrogenase(s) (IC50 = 20 microM) while stimul

114 Cytosolic retinol dehydrogenase(s) and the isozymes of alcohol deh

115 e short-chain dehydrogenase/reductase (SDR), retinol dehydrogenase-similar (RDH-S), with intense mRNA

116 ytes, we have identified two novel zebrafish retinol dehydrogenases, termed zRDHA and zRDHB, that sho

117 lls contain a membrane-bound NADPH-dependent retinol dehydrogenase that reacts efficiently with all-t

118 iously unrecognized physiologically relevant retinol dehydrogenases that contribute to retinoic acid

119 e therefore determined the topology of mouse retinol dehydrogenase type 1 (Rdh1) and cis-retinoid and

120 Mouse rdh1 encodes retinol dehydrogenase type 1 (RDH1), a short-chain dehyd

121 rt-chain dehydrogenase/reductase) rat RoDH1 (retinol dehydrogenase type 1) in the endoplasmic reticul

122 This study shows that microsomal retinol dehydrogenases, versus cytosolic retinol dehydro

123 Compensatory expression of other retinol dehydrogenases was observed in both Rdh5(-/-) an

124 At least two discrete forms of cytosolic retinol dehydrogenase were observed: NAD- and NADP-depen

125 viously unknown stereospecific enzyme, 9-cis-retinol dehydrogenase, which probably plays a role in 9-

126 tBP1 suppresses the expression of intestinal retinol dehydrogenases, which are required for retinoic