ライフサイエンスコーパス: reverse genetic

1 Y A(H1N1)pdm09 NA variants were generated by reverse genetics.

2 -CON strain was generated through the use of reverse genetics.

3 these viruses in a laboratory setting using reverse genetics.

4 ion against influenza virus by using in vivo reverse genetics.

5 s A/Ann Arbor/6/60 (AA ca) were generated by reverse genetics.

6 ific SSP functions via peptide treatment and reverse genetics.

7 All constructs were recovered by reverse genetics.

8 and diatoms 27 (CGLD27), by biochemistry or reverse genetics.

9 Using reverse genetics, 3 synergistically acting mutations wer

10 Using reverse genetics, a VP1 mutation (K244E) was shown to be

11 ng Rhizobium legume symbiosis (RLS)(8) or by reverse genetic analyses of differentially expressed can

12 Reverse genetic analyses of negative-strand RNA (NSR) vi

13 Reverse genetic analyses suggest that PVE1 functions dur

14 By using reverse genetic analyses, we identified altered use of h

15 stomatal movements have been investigated by reverse genetics analyses in Arabidopsis (Arabidopsis th

16 Reverse genetics analyses suggest that the structural mo

17 Live-cell imaging and reverse-genetic analyses of cp mutants in Arabidopsis (A

18 ta presented here will act as a resource for reverse genetic analysis and will be useful in decipheri

19 Reverse genetic analysis has failed to reveal abnormal p

20 To our knowledge, this represents the first reverse genetic analysis of a "novel" or heretofore poor

21 Reverse genetic analysis of several candidates identifie

22 Through reverse genetic analysis of the functions of four ripeni

23 important for wing-vein patterning and using reverse genetic analysis we identified five genes that s

24 The GWAS data were used to guide reverse genetic analysis, which found effectors of ABA a

25 d regulation and have also been subjected to reverse genetic analysis.

26 rate the accuracy of the predictions through reverse genetics analysis.

27 Reverse genetic and chromatin immunoprecipitation-PCR ap

28 atus SUN genes was investigated by combining reverse genetics and biochemistry.

29 ts, specific mutation in the viral genome by reverse genetics and confocal microscopy, here we demons

30 Using reverse genetics and fixing the CIV-H3N2 hemagglutinin (

31 wer of combining phylogenetic profiling with reverse genetics and gene-regulatory studies to identify

32 We used reverse genetics and heterologous expression to identify

33 s somatic embryo system and a combination of reverse genetics and microscopy to explore the roles of

34 gh-temporal-resolution micro-array analysis, reverse genetics and mRNA-seq.

35 , we used Arabidopsis (Arabidopsis thaliana) reverse genetics and multivariate long-term time-lapse i

36 al regulators of these 150 genes, so we used reverse genetics and pharmacologic methods to explore re

37 Reverse genetics and rescue of site-directed histidine m

38 tunity to introduce or reinforce concepts of reverse genetics and RNA interference, suppressor screen

39 Using reverse-genetic and biochemical approaches, we identifie

40 Our approach permits rapid, detailed reverse-genetic and chemical screens for effects on surv

41 Using reverse-genetic and chemical-genetic approaches, we dete

42 lity, the MuNoV system-with its native host, reverse genetics, and cell culture systems-will continue

43 perimental approaches including mutagenesis, reverse genetics, and growth kinetics, we found that thi

44 y NA in H9 virus replication was observed by reverse genetics, and recombinant H9N2 viruses with amin

45 YELLOW STRIPE-LIKE5) were further studied by reverse genetics, and their functional roles in the germ

46 ivatives and presents a general strategy for reverse genetic applications with other plant NSR viruse

47 -genome sequencing, providing a platform for reverse genetic applications.

48 tant strains, now available for forward- and reverse-genetic applications, and the introduction of a

49 To develop a reverse genetic approach for analysis of plant NSR virus

50 Utilizing a reverse genetic approach in Arabidopsis thaliana, we dem

51 Using a reverse genetic approach in Arabidopsis thaliana, we dem

52 Thus, this advance allows a reverse genetic approach in the axolotl and will undoubt

53 We used a reverse genetic approach to disrupt laminin expression i

54 By using a reverse genetic approach, we characterized the role of t

55 In a reverse genetic approach, we examined two independent he

56 Using a reverse genetic approach, we found that the GSL profile

57 animals using the SB transposon system in a reverse genetic approach.

58 role of GP shedding was investigated using a reverse genetics approach by comparing recombinant virus

59 A reverse genetics approach confirmed CAT4 to be responsib

60 isolates were searched for novel CFs using a reverse genetics approach followed by phenotypic analyse

61 A reverse genetics approach investigated the function of t

62 A reverse genetics approach on genes responsive to the pri

63 Here we employed a targeted reverse genetics approach to generate a pmpD null mutant

64 s contribute to pathogenesis, we exploited a reverse genetics approach to generate CE(NiP)DeltaP2-5,

65 Here, we utilized a reverse genetics approach to generate recombinant JUNV (

66 We took a reverse genetics approach to investigate whether GPX3 wo

67 A reverse genetics approach with genes responsive to the p

68 We took a reverse genetics approach, consisting of knock-out and c

69 Using a reverse genetics approach, our study clearly indicates t

70 Using a reverse genetics approach, we show that disruptions in t

71 Using a reverse genetics approach, we showed that, depending on

72 A reverse-genetics approach has been used to probe the mec

73 -interacting host proteins in planta using a reverse-genetics approach revealed a complex, molecular

74 Within the past 20 years, reverse genetic approaches have allowed direct determina

75 roughput sequencing has resulted in powerful reverse genetic approaches in polyploid crops.

76 Using a combination of forward and reverse genetic approaches on this line, we show here th

77 reeding or metabolic engineering) and enable reverse genetic approaches toward understanding the phys

78 Reverse genetic approaches utilize genetically engineere

79 2011 and 2012 demonstrated that forward and reverse genetic approaches were feasible with Chlamydia

80 Integrative bioinformatics and reverse genetic approaches were used to identify and val

81 We combined forward and reverse genetic approaches with chromatin immunoprecipit

82 Linkage analysis, reverse genetic approaches, and molecular complementatio

83 chemical, physiological, bioinformatics, and reverse genetic approaches, we analyzed how the flux of

84 econd, they are amenable to both forward and reverse genetic approaches, with a research toolset regu

85 was explored by pharmacological and forward/reverse genetic approaches.

86 st-growing needs in functional genomics, two reverse genetics approaches have been established: web-b

87 e novel and represent attractive targets for reverse genetics approaches to determine their roles in

88 Using reverse genetics approaches, the NSGT1-mediated glycosyl

89 Using independent forward and reverse genetics approaches, we demonstrate that PfAP2-G

90 Using pharmacological and reverse genetics approaches, we show that NAD-induced RO

91 ethylation were isolated through forward- or reverse-genetic approaches.

92 We used reverse-genetics approaches to rescue a battery of recom

93 The first successful attempts to apply reverse genetics based on the available metagenomic info

94 Here we exploited a reverse genetics-based replication-deficient BTV serotyp

95 Here we report the development of a novel reverse genetics-based system that allows the study of E

96 is thaliana) were further investigated using reverse genetic, biochemical, and immunological approach

97 ining transcriptome and expression data with reverse genetics, biochemistry, and metabolite profiling

98 Reverse genetic blocking of those pathways and a specifi

99 hnologies have vastly increased the power of reverse genetics but selection of candidate genes, from

100 is a model organism particularly suited for reverse genetics, but this inherent characteristic limit

101 A reverse genetics cell-based evaluation of genes linked t

102 A reverse genetics, cell-based, Good Manufacturing Practic

103 The efficacy of these reverse-genetic chemical probes has been demonstrated in

104 In this work, we have used reverse genetics coupled to biochemical and physiologica

105 atform for construction of more complex SYNV reverse genetic derivatives and presents a general strat

106 Remarkably, reverse-genetics-derived cell culture-adapted PEDVAVCT12

107 We sequentially passaged reverse-genetics-derived PR8 viruses with the K165E anti

108 Each mutant was successfully recovered by reverse genetics except for the one involving Ng6, which

109 ine, and have the potential to transform how reverse genetic experiments are performed in this import

110 Reverse genetic experiments found additional VLCFA and l

111 Reverse genetic experiments revealed that SAMHD1 phospho

112 In line with this notion, reverse genetics experiments supported an essential role

113 Reverse-genetics experiments demonstrated that the N145K

114 Although reverse genetics failed to identify a function for TBAs/

115 om the swine isolates in their pathogenesis, reverse genetics-generated reassortants between the swin

116 accines using inactivated wild-type virus or reverse genetics-generated vaccines containing the hemag

117 Although reverse genetics has been used to elucidate the function

118 us from bats or to generate these viruses by reverse genetics have failed to date.

119 2 genes of the novel H7N9 viruses created by reverse genetics in an important mammalian host model.

120 We used reverse genetics in combination with biochemical methods

121 man disease) demonstrates the power of mouse reverse genetics in mutational analysis of human genetic

122 ed signaling assays, confocal microscopy and reverse genetics/in vivo infection.

123 tions with mutant viruses generated by using reverse genetics indicated that the paired mutation of r

124 Through forward and reverse genetics it was shown that AtADS2 is involved in

125 /60 ca virus were generated by plasmid-based reverse genetics (Jap/57 ca, mal/78 ca, and sw/06 ca, re

126 In reverse genetic knockout (KO) studies that aim to assign

127 between the two proteins by biochemical and reverse genetics methods paves the way for rational drug

128 defense 1 (nad1) mutant was identified using reverse genetics methods.

129 We therefore adapted a reverse-genetics minigenome (MG) rescue system based on

130 This study describes the generation, using reverse genetics, of three different recombinant influen

131 With the use of reverse genetics on an avian H5N1 virus, we found that f

132 Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesi

133 le ligation-free cloning to rapidly generate reverse-genetics plasmids, which can be used for the res

134 In addition, the reverse genetics platform of the PC22A strain was furthe

135 In summary, we have developed two efficient reverse genetics platforms to facilitate functional char

136 To demonstrate the utility of the two reverse genetics platforms, two mutant alleles were isol

137 Further GWAS-guided reverse genetics promises to find additional effectors o

138 dy, we immunized mice with whole inactivated reverse genetics reassortant (RG) viruses expressing HA

139 With reverse genetics reassortants and point mutants of the t

140 cation, our findings also show that reovirus reverse genetics rescue is enhanced 100-fold by the NP86

141 mise as a cancer therapy, efficient reovirus reverse genetics rescue will accelerate production of re

142 opics that are discussed include Arabidopsis reverse genetic resources, stock centers, databases and

143 Comprehensive reverse genetic resources, which have been key to unders

144 mediary between functional genomics data and reverse-genetics resources for the genetic dissection of

145 2A or D62A E181A mutations into VHSV-IVb via reverse genetics resulted in viruses that replicated eff

146 Forward and reverse genetics revealed new information regarding unde

147 Reverse genetics revealed that val1 mutant seeds accumul

148 loropsis oceanica (NoDGAT2s or NoDGTTs), via reverse genetics, revealed that NoDGAT2A prefers saturat

149 libraries of mutant influenza viruses using reverse genetics (RG) and selected resistant variants in

150 oal, we first established a highly efficient reverse genetics (RG) system for AHSV serotype 1 (AHSV1)

151 e virus (BTV) has been studied by an in vivo reverse genetics (RG) system identifying the importance

152 e amino acid substitution in NA generated by reverse genetics (rg) that is associated with NAI resist

153 ecombinant wild-type (WT) virus generated by reverse genetics (rg-WT): rg-H274Y > rg-WT > rg-I222T >

154 rain A/chicken/Guangdong/1/1996 generated by reverse genetics (rg; rgGD/96), A/chicken/Legok/2003 (Le

155 t virus with the E119A mutation generated by reverse genetics [rg-E119A], rg-D198E, rg-I222T, rg-H274

156 Furthermore, a reverse genetic screen of a kinase-directed siRNA librar

157 Here, we report a reverse genetic screen spanning the ovarian transcriptom

158 We used a reverse genetic screen to identify Recognition of XopQ 1

159 this phenomenon, we carried out a systematic reverse genetic screen using a null allele of HMT1 and t

160 In a reverse genetic screen, we identified the chloroplast PP

161 , the first mutant analyzed in detail from a reverse genetic screen.

162 This reverse-genetic screen yielded eight mutants with root p

163 ing of barcoded mutants unlocks the power of reverse genetic screening for a malaria parasite and wil

164 Reverse genetic screening has been highly useful for det

165 n malaria parasites is hampered by a lack of reverse genetic screening methods.

166 nclude that CRISPR can be used as a powerful reverse genetic screening strategy in vivo in a vertebra

167 Here, we combine forward and reverse genetic screens to delineate a network of 184 ge

168 neered, they can be used for high-throughput reverse genetic screens to help functionally annotate th

169 We use the Haplobank in reverse genetic screens to investigate the temporal reso

170 ng transcriptional profiling, proteomics and reverse genetics screens provide detailed molecular desc

171 Reverse genetics showed functional redundancy of NHX1 an

172 Both forward and reverse genetic strategies have been used intensively to

173 Using a reverse genetic strategy in A. thaliana, we identified P

174 accumulation as a basis for a combined GWAS-reverse genetic strategy revealed the broad natural vari

175 Phenome-wide association is a novel reverse genetic strategy to analyze genome-to-phenome re

176 ification of candidate genes for forward and reverse genetic studies into the molecular mechanisms of

177 AV-mediated SpCas9 genome editing can enable reverse genetic studies of gene function in the brain.

178 Previous reverse genetic studies suggested that the roles of VRN1

179 , the utility of RNA interference (RNAi) for reverse genetic studies was demonstrated by targeting ge

180 h linker, a finding consistent with those of reverse genetics studies.

181 Here, in a reverse-genetics study with mouse hepatitis coronavirus,

182 Technical advances in reverse-genetics, such as RNA interference (RNAi), have

183 the tropism of aCFV, we used the geminiviral reverse genetic system and introduced a multimeric clone

184 Results of minigenome assays and an EBOV reverse genetic system rescue support a role for both th

185 This ZIKV reverse genetic system, together with mouse and mosquito

186 combinations at these three residues with a reverse genetics system and then investigated the molecu

187 There is no reverse genetics system available for the current epidem

188 lopment and characterization of a novel ZIKV reverse genetics system based on a 2015 isolate from Pue

189 e enhancing reovirus rescue from the current reverse genetics system by 100-fold.

190 The robust reverse genetics system described will be a valuable too

191 We constructed a reverse genetics system for avian paramyxovirus serotype

192 A newly described single-plasmid reverse genetics system for noroviruses has the potentia

193 To this end, we developed a plasmid-based reverse genetics system for Orsay virus by creating tran

194 an paramyxoviruses (APMVs), we constructed a reverse genetics system for recovery of infectious recom

195 A reverse genetics system for the highly virulent NDV stra

196 We generated a reverse genetics system for the live-attenuated MV vacci

197 This is the first plasmid-based virus reverse genetics system in the metazoan C. elegans.

198 Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent pro

199 Using a reverse genetics system of a prototypic arenavirus, Pich

200 ces, we first established a highly efficient reverse genetics system that increased rescue titers by

201 We developed a reverse genetics system to create a mutant of RRV (RRV(V

202 this study, we employed a helper virus-based reverse genetics system to identify NSP2 gene regions th

203 We have developed a reverse genetics system to produce live recombinant PICV

204 A reverse genetics system to rescue infectious Lujo virus

205 mmal switch in vitro, we first established a reverse genetics system to rescue UUKV with a genome clo

206 Moreover, the reverse genetics system we constructed will be helpful f

207 hese results indicate that the BAC-based MHV reverse genetics system will be useful for studies of JH

208 This optimized henipavirus reverse genetics system will facilitate future investiga

209 roup GII.3 strain U201 RNA, generated from a reverse genetics system, also does not induce an IFN res

210 sing Norwalk virus stool RNA transfection, a reverse genetics system, IFN neutralization reagents, an

211 Using a reverse genetics system, recombinant hMPVs (rhMPVs) lack

212 Compared to the classical reverse genetics system, the "eight-in-one" bacmids (bcm

213 Using the Pichinde virus (PICV) reverse genetics system, we analyzed the effects of alan

214 Using an IBV reverse genetics system, we demonstrated that the templa

215 Using the SARS-CoV reverse genetics system, we generated and characterized

216 Using a reverse genetics system, we generated recombinant RSV (r

217 Using the eight-plasmid reverse genetics system, we rescued wild-type SIV A/swin

218 terial artificial chromosome (BAC)-based MHV reverse genetics system.

219 nt Orsay virus, were generated by use of the reverse genetics system.

220 e amino acid mutation at this site through a reverse genetics system.

221 elp such investigations, we have developed a reverse-genetics system for UUKV that permits manipulati

222 Utilizing a reverse-genetics system recently developed, we report th

223 was evident after the development of the BTV reverse-genetics system that allows the introduction of

224 We established a reverse-genetics system to recover UUKV entirely from cD

225 By using the reverse-genetics system, we identified that 4 amino acid

226 ts, which facilitate recovery of MNV using a reverse-genetics system.

227 nological advances in glycan binding assays, reverse genetic systems for influenza and in X-ray cryst

228 We constructed reverse genetic systems for JHM.SD and JHM.WU and, utili

229 Animal models combined with reverse genetic systems of anellovirus have not been dev

230 The development of reverse genetics systems for wild-type strains of CDV an

231 ic biology approaches and the development of reverse genetics systems has allowed the rapid and relia

232 Using reverse genetics systems that we have recently developed

233 Therefore, the use of reverse genetics systems to engineer viruses lacking NSs

234 Here we report the development of reverse genetics systems to study STFSV replication and

235 Reverse genetics techniques have advanced to encompass t

236 To date, several reverse genetics techniques have been reported that harn

237 We used reverse genetics techniques to replace EBOV GP with its

238 Using reverse genetics techniques, we have developed a live-at

239 Using plasmid-based reverse genetics techniques, we have developed a single-

240 accines are available and the development of reverse genetic technologies has raised the prospect of

241 lycoproteins B (gB) and D (gD) of ILTV using reverse genetics technology.

242 ectious laryngotracheitis virus (ILTV) using reverse genetics technology.

243 MPV-C) as a bivalent vaccine candidate using reverse genetics technology.

244 strain and thermolabile LaSota strain using reverse genetics technology.

245 TILLING) provides a nontransgenic method for reverse genetics that is widely applicable, even in spec

246 tial enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to th

247 Here, we show, using reverse genetics, that a death-specific protein (DSP; pr

248 Using reverse genetics, the impact on growth and polymerase ac

249 Using biochemical assays and reverse genetics, the importance of conserved nsp7 and n

250 In this study, we implemented reverse genetics to address these obstacles with a multi

251 We have used reverse genetics to assess the significance of this regi

252 In this study, we utilized forward and reverse genetics to attempt to define which aspects of t

253 The combination of GWAS and reverse genetics to efficiently identify new effector ge

254 hat combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emerge

255 This study is the first of its kind using reverse genetics to evaluate the contribution of a C. tr

256 Here we use reverse genetics to examine the role of mutations in vir

257 tail in infectious virus production, we used reverse genetics to generate a recombinant influenza B v

258 Here, we used reverse genetics to generate a replication-incompetent i

259 genetic basis for these differences, we used reverse genetics to generate a series of reassortants of

260 Using reverse genetics to generate isogenic viruses with mutan

261 in the context of the intact virus, we used reverse genetics to generate Junin viruses (Candid #1 is

262 uenced sorghum mutant resource, we performed reverse genetics to identify eight genes potentially aff

263 We used RNA interference-based reverse genetics to inhibit the production of a structur

264 mmodate additional genetic sequence, we used reverse genetics to insert duplications (analogous to sy

265 entify determinants of transmission, we used reverse genetics to introduce gene segments of an early

266 We coupled bioinformatics analysis with reverse genetics to introduce mutations into RSV's negat

267 g polymorphism on viral fitness, we utilized reverse genetics to produce recombinant viruses encoding

268 r-wild-type (wt) MHV phenotypes when used by reverse genetics to replace its counterpart in the MHV g

269 To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned

270 We then used reverse genetics to rescue a recombinant LCMV (rLCMV) co

271 Here, we use reverse genetics to study the role of SSP myristoylation

272 We used reverse genetics to test the role of the small calcium-b

273 Custom-designed nucleases afford a powerful reverse genetic tool for direct gene disruption and geno

274 revealed several novel HR-related genes, and reverse genetics tools will allow us to understand the r

275 By using biochemical and reverse genetics tools, we have obtained strong evidence

276 al and chemical mutagenesis and move towards reverse-genetic tools as targeted genome editing.

277 The development of reverse-genetic tools in "nonmodel" insect species with

278 otein synthesis within the first 2 days of a reverse genetics transfection.

279 mutagenesis approach combining whole-genome reverse genetics using a set of tagged transposons and i

280 ted (ca) H3N8 vaccine virus was generated by reverse genetics using the wild-type (wt) hemagglutinin

281 Reverse genetics utilizing a full-length infectious clon

282 Reverse genetic viruses were generated from the classica

283 Reverse genetics was employed to examine the role of the

284 The Orsay virus reverse genetics we established will serve as a fundamen

285 Here, using reverse genetics, we comprehensively defined the impact

286 By using reverse genetics, we engineered recombinant PIV5-EGFP vi

287 Using reverse genetics, we engineered Ubl mutant viruses and f

288 Using reverse genetics, we examined the roles of six selected

289 By using reverse genetics, we found that a single amino acid at p

290 Using reverse genetics, we generated a panel of recombinant hM

291 Using reverse genetics, we generated a ToV-PLP knockout recomb

292 Using reverse genetics, we have generated a lead candidate onc

293 Using reverse genetics, we identified 2C residues that are nec

294 Here, using reverse genetics, we show that the K165E HA mutation dra

295 Using reverse genetics, we show that the ZIFL1.1 transporter r

296 Using CRISPR/Cas9 and RNAi reverse genetics, we show that TRH-like neuropeptides, t

297 Using reverse genetics, we showed ATF-2 to be functionally req

298 Viruses constructed by reverse genetics were engineered to contain converse PB2

299 Structure guided design and reverse genetics were used to sequentially transplant la

300 oduce a live attenuated candidate vaccine by reverse genetics with the hemagglutinin and neuraminidas