ライフサイエンスコーパス: reverse genetics

1 these viruses in a laboratory setting using reverse genetics.

2 ific SSP functions via peptide treatment and reverse genetics.

3 ion against influenza virus by using in vivo reverse genetics.

4 s A/Ann Arbor/6/60 (AA ca) were generated by reverse genetics.

5 All constructs were recovered by reverse genetics.

6 and diatoms 27 (CGLD27), by biochemistry or reverse genetics.

7 mined its in vivo function in red clover via reverse genetics.

8 ent and NV knockout recombinant VHSVs, using reverse genetics.

9 he RNA helicase domain of the NS3 protein by reverse genetics.

10 t-Sk-1/07 (Tok07, H3N2) influenza viruses by reverse genetics.

11 tations into the OTU domain region by use of reverse genetics.

12 nn Arbor/6/60 (H2N2) virus were generated by reverse genetics.

13 Y A(H1N1)pdm09 NA variants were generated by reverse genetics.

14 -CON strain was generated through the use of reverse genetics.

15 Using reverse genetics, 3 synergistically acting mutations wer

16 Using reverse genetics, a modified BTV-1 that expresses the fl

17 Using reverse genetics, a VP1 mutation (K244E) was shown to be

18 Using reverse genetics, an A/Puerto Rico/8/34 (A/PR/8/34) viru

19 stomatal movements have been investigated by reverse genetics analyses in Arabidopsis (Arabidopsis th

20 Reverse genetics analyses suggest that the structural mo

21 rate the accuracy of the predictions through reverse genetics analysis.

22 ion of infectious virus particles using both reverse genetics and a protein trans-complementation ass

23 atus SUN genes was investigated by combining reverse genetics and biochemistry.

24 ts, specific mutation in the viral genome by reverse genetics and confocal microscopy, here we demons

25 e (RNAi) has become a powerful technique for reverse genetics and drug discovery, and in both of thes

26 sed pathogenesis of MA15 in rodents, we used reverse genetics and evaluated the virulence of panels o

27 Using reverse genetics and fixing the CIV-H3N2 hemagglutinin (

28 wer of combining phylogenetic profiling with reverse genetics and gene-regulatory studies to identify

29 We used reverse genetics and heterologous expression to identify

30 By reverse genetics and metabolic labeling experiments, we

31 s somatic embryo system and a combination of reverse genetics and microscopy to explore the roles of

32 gh-temporal-resolution micro-array analysis, reverse genetics and mRNA-seq.

33 , we used Arabidopsis (Arabidopsis thaliana) reverse genetics and multivariate long-term time-lapse i

34 al regulators of these 150 genes, so we used reverse genetics and pharmacologic methods to explore re

35 Reverse genetics and rescue of site-directed histidine m

36 tunity to introduce or reinforce concepts of reverse genetics and RNA interference, suppressor screen

37 onsin/14094/99 (H3N2) virus was generated by reverse genetics and subsequently mutated in the PB1 and

38 Gene targeting is indispensible for reverse genetics and the generation of animal models of

39 different influenza subtypes, was rescued by reverse genetics, and a comparative study of immune resp

40 lity, the MuNoV system-with its native host, reverse genetics, and cell culture systems-will continue

41 perimental approaches including mutagenesis, reverse genetics, and growth kinetics, we found that thi

42 y NA in H9 virus replication was observed by reverse genetics, and recombinant H9N2 viruses with amin

43 YELLOW STRIPE-LIKE5) were further studied by reverse genetics, and their functional roles in the germ

44 role of GP shedding was investigated using a reverse genetics approach by comparing recombinant virus

45 A reverse genetics approach confirmed CAT4 to be responsib

46 isolates were searched for novel CFs using a reverse genetics approach followed by phenotypic analyse

47 A reverse genetics approach investigated the function of t

48 A reverse genetics approach on genes responsive to the pri

49 Here we employed a targeted reverse genetics approach to generate a pmpD null mutant

50 s contribute to pathogenesis, we exploited a reverse genetics approach to generate CE(NiP)DeltaP2-5,

51 Here, we utilized a reverse genetics approach to generate recombinant JUNV (

52 We employed a reverse genetics approach to identify putative SO(4)(2-)

53 We took a reverse genetics approach to investigate whether GPX3 wo

54 bidopsis Xa21 functional homologs, we used a reverse genetics approach to screen T-DNA insertion muta

55 In this study, we used a reverse genetics approach to study the role of Pht1;5 in

56 A reverse genetics approach with genes responsive to the p

57 ations were introduced into SARS-CoV using a reverse genetics approach, and the G100E and G104E mutat

58 We took a reverse genetics approach, consisting of knock-out and c

59 Using a reverse genetics approach, our study clearly indicates t

60 Using a reverse genetics approach, we explored the roles of Arab

61 Using a reverse genetics approach, we show that disruptions in t

62 Using a reverse genetics approach, we showed that, depending on

63 e (RNAi) led to a much greater reliance on a reverse genetics approach: reducing the function of know

64 A reverse-genetics approach has been used to probe the mec

65 -interacting host proteins in planta using a reverse-genetics approach revealed a complex, molecular

66 st-growing needs in functional genomics, two reverse genetics approaches have been established: web-b

67 e novel and represent attractive targets for reverse genetics approaches to determine their roles in

68 Using reverse genetics approaches, the NSGT1-mediated glycosyl

69 Using independent forward and reverse genetics approaches, we demonstrate that PfAP2-G

70 Using pharmacological and reverse genetics approaches, we show that NAD-induced RO

71 mutation to the Clone 13 phenotype, we used reverse-genetics approaches to generate recombinant LCM

72 e an important resource in both forward- and reverse-genetics approaches to identify hundreds of muta

73 We used reverse-genetics approaches to rescue a battery of recom

74 The first successful attempts to apply reverse genetics based on the available metagenomic info

75 Here we exploited a reverse genetics-based replication-deficient BTV serotyp

76 Here we report the development of a novel reverse genetics-based system that allows the study of E

77 ining transcriptome and expression data with reverse genetics, biochemistry, and metabolite profiling

78 hnologies have vastly increased the power of reverse genetics but selection of candidate genes, from

79 is a model organism particularly suited for reverse genetics, but this inherent characteristic limit

80 used libraries of biased chemical probes and reverse genetics by RNA interference, we have identified

81 d.msu.edu) seeks synergy between forward and reverse genetics by screening thousands of sequence-inde

82 A reverse genetics cell-based evaluation of genes linked t

83 A reverse genetics, cell-based, Good Manufacturing Practic

84 Using reverse genetics, complementation analyses, and cell bio

85 In this work, we have used reverse genetics coupled to biochemical and physiologica

86 Remarkably, reverse-genetics-derived cell culture-adapted PEDVAVCT12

87 We sequentially passaged reverse-genetics-derived PR8 viruses with the K165E anti

88 63%-sequence-identical 210-nt BCoV 5' UTR by reverse genetics does not yield viable virus, suggesting

89 Each mutant was successfully recovered by reverse genetics except for the one involving Ng6, which

90 In line with this notion, reverse genetics experiments supported an essential role

91 The gene expression map should guide future reverse genetics experiments, high-resolution analyses o

92 Reverse-genetics experiments demonstrated that the N145K

93 Although reverse genetics failed to identify a function for TBAs/

94 om the swine isolates in their pathogenesis, reverse genetics-generated reassortants between the swin

95 accines using inactivated wild-type virus or reverse genetics-generated vaccines containing the hemag

96 Here, we demonstrate a robust system for the reverse genetics generation of a RVF virus replicon part

97 ional analysis of this family by forward and reverse genetics has been hampered by genetic redundancy

98 Although reverse genetics has been used to elucidate the function

99 us from bats or to generate these viruses by reverse genetics have failed to date.

100 RNAi has revolutionized reverse genetics; however, RNAi is not necessarily ubiqu

101 a glycan-binding site in the MNV-1 capsid by reverse genetics identified a region topologically simil

102 2 genes of the novel H7N9 viruses created by reverse genetics in an important mammalian host model.

103 In this study, we used forward and reverse genetics in Arabidopsis to isolate four pex14 al

104 We used reverse genetics in combination with biochemical methods

105 We discuss the utility of Ds for forward and reverse genetics in maize and provide evidence that gene

106 man disease) demonstrates the power of mouse reverse genetics in mutational analysis of human genetic

107 has been highly successful in parallelizing reverse genetics in Saccharomyces cerevisiae and has led

108 ed signaling assays, confocal microscopy and reverse genetics/in vivo infection.

109 tions with mutant viruses generated by using reverse genetics indicated that the paired mutation of r

110 Reverse genetics is also an excellent way to associate g

111 al validation of drug and vaccine targets by reverse genetics is constrained by the inefficiency of h

112 Through forward and reverse genetics it was shown that AtADS2 is involved in

113 /60 ca virus were generated by plasmid-based reverse genetics (Jap/57 ca, mal/78 ca, and sw/06 ca, re

114 We used this reverse genetics method to generate a panel of viruses w

115 hould permit the extension of this "two-hit" reverse genetics methodology to other RV genes.

116 al selection strategy to previously reported reverse genetics methods for RV may enhance the efficien

117 between the two proteins by biochemical and reverse genetics methods paves the way for rational drug

118 defense 1 (nad1) mutant was identified using reverse genetics methods.

119 We therefore adapted a reverse-genetics minigenome (MG) rescue system based on

120 This study describes the generation, using reverse genetics, of three different recombinant influen

121 With the use of reverse genetics on an avian H5N1 virus, we found that f

122 Using reverse genetics, partial in-frame deletions of p7 were

123 oject, an Arabidopsis (Arabidopsis thaliana) reverse genetics phenotypic screen of more than 5,000 mu

124 Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesi

125 le ligation-free cloning to rapidly generate reverse-genetics plasmids, which can be used for the res

126 In addition, the reverse genetics platform of the PC22A strain was furthe

127 In summary, we have developed two efficient reverse genetics platforms to facilitate functional char

128 To demonstrate the utility of the two reverse genetics platforms, two mutant alleles were isol

129 nities not encountered in typical forward or reverse genetics projects.

130 Further GWAS-guided reverse genetics promises to find additional effectors o

131 dy, we immunized mice with whole inactivated reverse genetics reassortant (RG) viruses expressing HA

132 With reverse genetics reassortants and point mutants of the t

133 cation, our findings also show that reovirus reverse genetics rescue is enhanced 100-fold by the NP86

134 mise as a cancer therapy, efficient reovirus reverse genetics rescue will accelerate production of re

135 mediary between functional genomics data and reverse-genetics resources for the genetic dissection of

136 2A or D62A E181A mutations into VHSV-IVb via reverse genetics resulted in viruses that replicated eff

137 Forward and reverse genetics revealed new information regarding unde

138 Reverse genetics revealed that the PL2 trans-cleavage ac

139 Reverse genetics revealed that val1 mutant seeds accumul

140 loropsis oceanica (NoDGAT2s or NoDGTTs), via reverse genetics, revealed that NoDGAT2A prefers saturat

141 libraries of mutant influenza viruses using reverse genetics (RG) and selected resistant variants in

142 oal, we first established a highly efficient reverse genetics (RG) system for AHSV serotype 1 (AHSV1)

143 e virus (BTV) has been studied by an in vivo reverse genetics (RG) system identifying the importance

144 polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine k

145 e amino acid substitution in NA generated by reverse genetics (rg) that is associated with NAI resist

146 ecombinant wild-type (WT) virus generated by reverse genetics (rg-WT): rg-H274Y > rg-WT > rg-I222T >

147 rain A/chicken/Guangdong/1/1996 generated by reverse genetics (rg; rgGD/96), A/chicken/Legok/2003 (Le

148 t virus with the E119A mutation generated by reverse genetics [rg-E119A], rg-D198E, rg-I222T, rg-H274

149 ng transcriptional profiling, proteomics and reverse genetics screens provide detailed molecular desc

150 Reverse genetics showed functional redundancy of NHX1 an

151 Of importance, reverse genetics shows that alpha-catenin and delta-cate

152 A kinome-wide reverse genetics strategy identified 36 parasite kinases

153 h linker, a finding consistent with those of reverse genetics studies.

154 Here, in a reverse-genetics study with mouse hepatitis coronavirus,

155 Technical advances in reverse-genetics, such as RNA interference (RNAi), have

156 By taking advantage of the available reverse genetics system and ectopic expression system fo

157 combinations at these three residues with a reverse genetics system and then investigated the molecu

158 There is no reverse genetics system available for the current epidem

159 lopment and characterization of a novel ZIKV reverse genetics system based on a 2015 isolate from Pue

160 Therefore, we developed a reverse genetics system based on the neurovirulent Snyde

161 e Ebola virus (ZEBOV) glycoprotein (GP) by a reverse genetics system based on the SAD B19 RABV wildli

162 e enhancing reovirus rescue from the current reverse genetics system by 100-fold.

163 ed human RV isolate, demonstrating that this reverse genetics system can be used to study the molecul

164 The robust reverse genetics system described will be a valuable too

165 By taking advantage of the available reverse genetics system for a model arenavirus, Pichinde

166 We constructed a reverse genetics system for avian paramyxovirus serotype

167 e and developed the first, to our knowledge, reverse genetics system for HRV-C.

168 A newly described single-plasmid reverse genetics system for noroviruses has the potentia

169 To this end, we developed a plasmid-based reverse genetics system for Orsay virus by creating tran

170 an paramyxoviruses (APMVs), we constructed a reverse genetics system for recovery of infectious recom

171 In this study, we constructed a reverse genetics system for recovery of infectious recom

172 A reverse genetics system for the highly virulent NDV stra

173 We generated a reverse genetics system for the live-attenuated MV vacci

174 eliminating potential acceptor sites using a reverse genetics system for the moderately pathogenic st

175 This is the first plasmid-based virus reverse genetics system in the metazoan C. elegans.

176 Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent pro

177 Using a reverse genetics system of a prototypic arenavirus, Pich

178 ces, we first established a highly efficient reverse genetics system that increased rescue titers by

179 We developed a reverse genetics system to create a mutant of RRV (RRV(V

180 this study, we employed a helper virus-based reverse genetics system to identify NSP2 gene regions th

181 n was confirmed using a vaccinia virus-based reverse genetics system to produce a recombinant virus,

182 We have developed a reverse genetics system to produce live recombinant PICV

183 ingle cycle (DISC) vaccine strain, we used a reverse genetics system to rescue defective virus strain

184 A reverse genetics system to rescue infectious Lujo virus

185 We report the development of an efficient reverse genetics system to rescue recombinant LASV and t

186 mmal switch in vitro, we first established a reverse genetics system to rescue UUKV with a genome clo

187 The reverse genetics system was used to analyze the role of

188 Moreover, the reverse genetics system we constructed will be helpful f

189 hese results indicate that the BAC-based MHV reverse genetics system will be useful for studies of JH

190 This optimized henipavirus reverse genetics system will facilitate future investiga

191 roup GII.3 strain U201 RNA, generated from a reverse genetics system, also does not induce an IFN res

192 sing Norwalk virus stool RNA transfection, a reverse genetics system, IFN neutralization reagents, an

193 By using a cDNA-based reverse genetics system, insertions or substitutions of

194 Using a reverse genetics system, recombinant hMPVs (rhMPVs) lack

195 Compared to the classical reverse genetics system, the "eight-in-one" bacmids (bcm

196 Using the Pichinde virus (PICV) reverse genetics system, we analyzed the effects of alan

197 Using an IBV reverse genetics system, we demonstrated that the templa

198 Using the SARS-CoV reverse genetics system, we generated and characterized

199 Using a reverse genetics system, we generated recombinant RSV (r

200 Using the eight-plasmid reverse genetics system, we rescued wild-type SIV A/swin

201 terial artificial chromosome (BAC)-based MHV reverse genetics system.

202 nt Orsay virus, were generated by use of the reverse genetics system.

203 e amino acid mutation at this site through a reverse genetics system.

204 ies of the virus S and L segments by using a reverse genetics system.

205 In this study, we constructed a reverse-genetics system for recovery of infectious APMV-

206 elp such investigations, we have developed a reverse-genetics system for UUKV that permits manipulati

207 Utilizing a reverse-genetics system recently developed, we report th

208 was evident after the development of the BTV reverse-genetics system that allows the introduction of

209 We established a reverse-genetics system to recover UUKV entirely from cD

210 By using the reverse-genetics system, we identified that 4 amino acid

211 ts, which facilitate recovery of MNV using a reverse-genetics system.

212 The development of reverse genetics systems for wild-type strains of CDV an

213 ic biology approaches and the development of reverse genetics systems has allowed the rapid and relia

214 Using reverse genetics systems that we have recently developed

215 Therefore, the use of reverse genetics systems to engineer viruses lacking NSs

216 Here we report the development of reverse genetics systems to study STFSV replication and

217 urine norovirus (MNV) and the development of reverse-genetics systems for this virus provide an oppor

218 Reverse genetics techniques have advanced to encompass t

219 To date, several reverse genetics techniques have been reported that harn

220 We used reverse genetics techniques to replace EBOV GP with its

221 Using reverse genetics techniques, we have developed a live-at

222 Using plasmid-based reverse genetics techniques, we have developed a single-

223 The reverse genetics technology for bluetongue virus (BTV) h

224 lycoproteins B (gB) and D (gD) of ILTV using reverse genetics technology.

225 ectious laryngotracheitis virus (ILTV) using reverse genetics technology.

226 MPV-C) as a bivalent vaccine candidate using reverse genetics technology.

227 strain and thermolabile LaSota strain using reverse genetics technology.

228 TILLING) provides a nontransgenic method for reverse genetics that is widely applicable, even in spec

229 tial enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to th

230 Here, we show, using reverse genetics, that a death-specific protein (DSP; pr

231 Using reverse genetics, the impact on growth and polymerase ac

232 Using biochemical assays and reverse genetics, the importance of conserved nsp7 and n

233 Using reverse genetics, three recombinant Newcastle disease vi

234 In this study, we implemented reverse genetics to address these obstacles with a multi

235 phospho-proteomic analysis with kinome-wide reverse genetics to assess the importance of protein pho

236 We have used reverse genetics to assess the significance of this regi

237 In this study, we utilized forward and reverse genetics to attempt to define which aspects of t

238 on, we have used RNA interference (RNAi) and reverse genetics to define functional domains in two inc

239 The combination of GWAS and reverse genetics to efficiently identify new effector ge

240 hat combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emerge

241 This study is the first of its kind using reverse genetics to evaluate the contribution of a C. tr

242 Here we use reverse genetics to examine the role of mutations in vir

243 tail in infectious virus production, we used reverse genetics to generate a recombinant influenza B v

244 Here, we used reverse genetics to generate a replication-incompetent i

245 genetic basis for these differences, we used reverse genetics to generate a series of reassortants of

246 Using reverse genetics to generate isogenic viruses with mutan

247 in the context of the intact virus, we used reverse genetics to generate Junin viruses (Candid #1 is

248 uenced sorghum mutant resource, we performed reverse genetics to identify eight genes potentially aff

249 We used RNA interference-based reverse genetics to inhibit the production of a structur

250 mmodate additional genetic sequence, we used reverse genetics to insert duplications (analogous to sy

251 ects of dec-RRLL-CMK, we employed arenavirus reverse genetics to introduce a furin recognition site i

252 entify determinants of transmission, we used reverse genetics to introduce gene segments of an early

253 We coupled bioinformatics analysis with reverse genetics to introduce mutations into RSV's negat

254 g polymorphism on viral fitness, we utilized reverse genetics to produce recombinant viruses encoding

255 r-wild-type (wt) MHV phenotypes when used by reverse genetics to replace its counterpart in the MHV g

256 To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned

257 We then used reverse genetics to rescue a recombinant LCMV (rLCMV) co

258 We used reverse genetics to rescue a recombinant LCMV strain car

259 To this end, we used reverse genetics to rescue the pathogenic Romero and the

260 Here, we use reverse genetics to study the role of SSP myristoylation

261 We used reverse genetics to test the role of the small calcium-b

262 S), they also have important applications as reverse genetics tools for gene function studies.

263 e recent availability of genome sequence and reverse genetics tools for model monocots and basal land

264 revealed several novel HR-related genes, and reverse genetics tools will allow us to understand the r

265 By using biochemical and reverse genetics tools, we have obtained strong evidence

266 otein synthesis within the first 2 days of a reverse genetics transfection.

267 mutagenesis approach combining whole-genome reverse genetics using a set of tagged transposons and i

268 7N7 candidate vaccine virus was generated by reverse genetics using the modified hemagglutinin (HA) a

269 ted (ca) H3N8 vaccine virus was generated by reverse genetics using the wild-type (wt) hemagglutinin

270 Here, we apply reverse genetics, using knock-outs of the relevant diter

271 Reverse genetics utilizing a full-length infectious clon

272 Reverse genetics was employed to examine the role of the

273 The Orsay virus reverse genetics we established will serve as a fundamen

274 Using reverse genetics, we attempted the recovery of a panel o

275 Using reverse genetics, we attempted to generate 74 viruses po

276 Here, using reverse genetics, we comprehensively defined the impact

277 Using reverse genetics, we demonstrate here that modifications

278 In an effort to expand the utility of RV reverse genetics, we developed a method to recover recom

279 By using reverse genetics, we engineered recombinant PIV5-EGFP vi

280 Using reverse genetics, we engineered Ubl mutant viruses and f

281 Using reverse genetics, we examined the roles of six selected

282 By using reverse genetics, we found that a single amino acid at p

283 Using reverse genetics, we generated a panel of recombinant hM

284 Using reverse genetics, we generated a recombinant virus, desi

285 Using reverse genetics, we generated a ToV-PLP knockout recomb

286 Using reverse genetics, we generated recombinant viruses that

287 Using reverse genetics, we have generated a lead candidate onc

288 Using reverse genetics, we identified 2C residues that are nec

289 Using methods of reverse genetics, we recently identified VentX, a human

290 Using reverse genetics, we show that AtD27 is required for the

291 Here, using reverse genetics, we show that the K165E HA mutation dra

292 Using reverse genetics, we show that the ZIFL1.1 transporter r

293 Using CRISPR/Cas9 and RNAi reverse genetics, we show that TRH-like neuropeptides, t

294 Using reverse genetics, we show that whereas single knockouts

295 Using reverse genetics, we show that, unlike the single knocko

296 Using reverse genetics, we showed ATF-2 to be functionally req

297 Viruses constructed by reverse genetics were engineered to contain converse PB2

298 Structure guided design and reverse genetics were used to sequentially transplant la

299 Employing reverse genetics, where PXR has been genetically deleted

300 oduce a live attenuated candidate vaccine by reverse genetics with the hemagglutinin and neuraminidas